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At least 37 records · Page 2

Geology and elevation shape bacterial assembly in Antarctic endolithic communities

Ice free areas of continental Antarctica are among the coldest and driest environments on Earth, and yet, they support surprisingly diverse and highly adapted microbial communities. Endolithic growth is one of the key adaptations to such extreme environments and often represents the dominant life-form. Despite growing scientific interest, little is known of the mechanisms that influence the assembly of endolithic microbiomes across these harsh environments. Here, we used metagenomics to examine the diversity and assembly of endolithic bacterial communities across Antarctica within different rock types and over a large elevation range. While granite supported richer and more heterogeneous communities than sandstone, elevation had no apparent effect on taxonomic richness, regardless of rock type. Conversely, elevation was clearly associated with turnover in community composition, with the deterministic process of variable selection driving microbial assembly along the elevation gradient. The turnover associated with elevation was modulated by geology, whereby for a given elevation difference, turnover was consistently larger between communities inhabiting different rock types. Overall, selection imposed by elevation and geology appeared stronger than turnover related to other spatially-structured environmental drivers. Our findings indicate that at the cold-arid limit of life on Earth, geology and elevation are key determinants of endolithic bacterial heterogeneity. This also suggests that warming temperatures may threaten the persistence of such extreme-adapted organisms.

54 ENVIRONMENTAL SCIENCES↗

Groundwater elevation data for monitoring wells within the East and Taylor River basins, Colorado (USA)

This dataset is comprised of temporal variations in groundwater elevation data for the 24 monitoring wells located throughout the East River watershed. Seasonal to annual variations in groundwater elevations are a critical property of mountainous watersheds needed to understand both hydrological and below ground biogeochemical processes. Such data serve as a critical constraint for numerical models describing coupled groundwater-surface water behavior within the watershed. Additionally, the offset between the maximum and minimum groundwater elevations defines the extent of the bedrock weathering zone, with annual excursions in the groundwater hydrographic (i.e., the rising and falling hydrographic limbs) imposing primary controls on bedrock saturation state and redox conditions that govern biogeochemical reactions impacting nitrogen, carbon, and metals cycling. Manufacturer-specific software is used to download pressure data from each transducer, with broadly available spreadsheet software (e.g. Microsoft Excel) used to convert temporal variations in water pressure to elevations in units of meters above mean sea level. As additional monitoring wells are installed within the East River watershed and new groundwater monitoring wells are installed in the Taylor River watershed, temporal groundwater elevation data will be included as a part of this master dataset. Details regarding the metadata associated with each monitoring well location, including well depths, screened intervals, well location coordinates, and bedrock type, are included, as is a standard operating procedure for generating groundwater elevation data from water pressure values recorded by the pressure transducers. This dataset includes: (1) a zip file (East_River_Watershed_Compiled_Groundwater_Elevation_Data_Plots.zip), containing (a) PNG of groundwater hydrographs, (b) a CSV file with groundwater elevation data, and (c) CSV file containing metadata organized by location; (2) an Excel file (East_River_Watershed_Compiled_Groundwater_Elevation_Data_Plots.xlsx) with the groundwater elevation data, groundwater hydrographs, and metadata organized by location; (3) a Word file (Groundwater_elevation_data_protocols.docx) and a PDF file version (Groundwater_elevation_data_protocols.pdf) containing field protocols and methods; (4) a location metadata (locations.csv) file; (5) a file level metadata (flmd.csv); and (6) data dictionary (dd.csv) file.This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

54 ENVIRONMENTAL SCIENCES↗

Carbon accumulation rate peaks at 1,000-m elevation in tropical planted and regrowth forests

Tropical planted and regrowth forests (TPRFs) are one of the most low-cost components for recovering biomass-stored carbon in the tropics. Nevertheless, challenges persist in pinpointing which elevational ranges exhibit the largest carbon accumulation rate ($γ$ rapid ) due to the highly inconsistent previous assessments. This prevents the selection of optimal locations for implementing large-scale reforestation in the tropics. Here, in this study, we proposed a refined approach that used a carbon accumulation threshold (<80% of the maximum value) to quantify $γ$ rapid in TPRFs at various elevations. We find that $γ$ rapid increases with elevations from 300 to 1,000 m and declines at elevations >1,000 m. TPRFs at elevations ~1,000 m exhibit three times more $γ$ rapid than lowland TPRFs. This optimal elevation, highly dependent on background temperatures, varies slightly but significantly across different mountains. These findings provide guidelines for policymakers to determine the optimal elevations from regional to continental scales when implementing reforestation initiatives in the tropics.

54 ENVIRONMENTAL SCIENCES↗

Parameterization of the Elevated Convection With a Unified Convection Scheme (UNICON) and Its Impacts on the Diurnal Cycle of Precipitation

To improve the simulation of nocturnal precipitation, we develop a parameterization for the elevated convection that is launched from the level of maximum grid-mean moist static energy, when grid-mean vertical flow is upward at the launching interface. The parameterized elevated convection is forced by both cold pool-driven and partially resolved external mesoscale organized flows. Properties of the external mesoscale flow are estimated from grid-mean vertical velocity and three dimensional advection tendencies of temperature and moisture. The new parameterization is implemented into a unified convection scheme (UNICON) and tested for both the single-column case at the Southern Great Plain (SGP) site in US and in global simulations. At the SGP site, the parameterized elevated convection strengthens nocturnal convection, increases nocturnal precipitation, and better simulates the observed diurnal cycle of precipitation. It appears that the elevated and surface-based convections interact with each other by stabilizing the atmospheric column, which affects subsequent convection. Global simulation shows that the elevated convection mostly occurs over the continents during the night, and also over the oceanic mid-latitude warm air advection and storm track regions during summer. Without degrading global mean climate, the elevated convection improves the simulation of nocturnal precipitation in the mid-US but simulates somewhat strong nocturnal precipitation in northeastern Asia. It seems that a key to simulate observed nocturnal precipitation is to appropriately parameterize the impacts of external organized flow on the elevated convection.

54 ENVIRONMENTAL SCIENCES↗

Trading water for carbon in the future: Effects of elevated CO 2 and warming on leaf hydraulic traits in a semiarid grassland

Abstract The effects of climate change on plants and ecosystems are mediated by plant hydraulic traits, including interspecific and intraspecific variability of trait phenotypes. Yet, integrative and realistic studies of hydraulic traits and climate change are rare. In a semiarid grassland, we assessed the response of several plant hydraulic traits to elevated CO 2 (+200 ppm) and warming (+1.5 to 3°C; day to night). For leaves of five dominant species (three graminoids and two forbs), and in replicated plots exposed to 7 years of elevated CO 2 , warming, or ambient climate, we measured: stomatal density and size, xylem vessel size, turgor loss point, and water potential (pre‐dawn). Interspecific differences in hydraulic traits were larger than intraspecific shifts induced by elevated CO 2 and/or warming. Effects of elevated CO 2 were greater than effects of warming, and interactions between treatments were weak or not detected. The forbs showed little phenotypic plasticity. The graminoids had leaf water potentials and turgor loss points that were 10% to 50% less negative under elevated CO 2 ; thus, climate change might cause these species to adjust their drought resistance strategy away from tolerance and toward avoidance. The C4 grass also reduced allocation of leaf area to stomata under elevated CO 2 , which helps explain observations of higher soil moisture. The shifts in hydraulic traits under elevated CO 2 were not, however, simply due to higher soil moisture. Integration of our results with others' indicates that common species in this grassland are more likely to adjust stomatal aperture in response to near‐term climate change, rather than anatomical traits; this contrasts with apparent effects of changing CO 2 on plant anatomy over evolutionary time. Future studies should assess how plant responses to drought may be constrained by the apparent shift from tolerance (via low turgor loss point) to avoidance (via stomatal regulation and/or access to deeper soil moisture).

54 ENVIRONMENTAL SCIENCES↗

Interactions between macro‐ and micro‐climate: Effects on phenolic compound production in Nardus stricta at high elevations

Abstract Phenolic compounds are key to plant defence, offering protection as antioxidants, UV shields, and antimicrobials. Their production is largely shaped by environmental conditions. It is believed that plants at lower elevations increase phenolic content to counter herbivory, while those at higher elevations rely on phenolics to manage abiotic stresses, such as climate variability. Microhabitat warming also affects phenolic levels, but responses differ, depending on broader climatic contexts: plants in warmer, lower‐elevation environments show limited adaptability, whereas high‐elevation plants demonstrate greater plasticity. Despite the importance of these environmental interactions, many small‐scale abiotic studies lack sufficient spatial replication across broader gradients like elevation or latitude, while large‐scale studies frequently overlook microscale factors. This study investigated the effects of macroclimate factors and microhabitat warming on phenolic production in Nardus stricta across five semi‐natural grassland sites (1546–1875 m a.s.l.) in Portugal's Serra da Estrela. Warming was simulated using open‐top chambers over two growing seasons, after which leaf samples were analysed for phenolic compounds, and soil nutrients were measured. The N. stricta plants at the highest elevation site contained significantly higher leaf flavonoid concentrations. Microhabitat warming led to a significant decrease in flavonoid concentrations, but only at the highest elevation site. These effects occurred independently of soil nutrient levels, suggesting direct thermal effects or stress responses might be involved. Our findings highlight the complex interactions between macro‐ and microenvironmental factors in shaping plant chemistry, underscoring critical considerations for plant resilience in the face of climate change. This understanding is essential for developing strategies to support plant and ecosystem adaptation to changing climates.

Moreira, X. [Misión Biológica de Galicia (MBG‐CSIC↗

An Integrative Model for Soil Biogeochemistry and Methane Processes. II: Warming and Elevated CO 2 Effects on Peatland CH 4 Emissions

Peatlands are one of the largest natural sources for atmospheric methane (CH 4 ), a potent greenhouse gas. Climate warming and elevated atmospheric carbon dioxide (CO 2 ) are two important environmental factors that have been confirmed to stimulate peatland CH 4 emissions; however, the mechanisms underlying enhanced emissions remain elusive. A data-model integration approach was applied to understand the CH 4 processes in a northern temperate peatland under a gradient of warming and doubled atmospheric CO 2 concentration. We found that warming and elevated CO 2 stimulated CH 4 emissions through different mechanisms. Warming initially stimulated but then suppressed vegetative productivity while stimulating soil organic matter (SOM) mineralization and dissolved organic carbon (DOC) fermentation, which led to higher acetate production and enhanced acetoclastic and hydrogenotrophic methanogenesis. Warming also enhanced surface CH 4 emissions, which combined with warming-caused decreases in CH 4 solubility led to slightly lower dissolved CH 4 concentrations through the soil profiles. Elevated CO 2 enhanced ecosystem productivity and SOM mineralization, resulting in higher DOC and acetate concentrations. Higher DOC and acetate concentrations increased acetoclastic and hydrogenotrophic methanogenesis and led to higher dissolved CH 4 concentrations and CH 4 emissions. Both warming and elevated CO 2 had minor impacts on CH 4 oxidation. A meta-analysis of warming and elevated CO 2 impacts on carbon cycling in wetlands agreed well with a majority of the modeled mechanisms. Finally, this mechanistic understanding of the stimulating impacts of warming and elevated CO 2 on peatland CH 4 emissions enhances our predictability on the climate-ecosystem feedback.

54 ENVIRONMENTAL SCIENCES↗

How do drought and heat affect the response of soybean seed yield to elevated O 3 ? An analysis of 15 seasons of free–air O 3 concentration enrichment studies

The coincidence of rising ozone concentrations ([O 3 ]), increasing global temperatures, and drought episodes is expected to become more intense and frequent in the future. A better understanding of the responses of crop yield to elevated [O 3 ] under different levels of drought and high temperature stress is, therefore, critical for projecting future food production potential. Using a 15-year open-air field experiment in central Illinois, we assessed the impacts of elevated [O 3 ] coupled with variation in growing season temperature and water availability on soybean seed yield. Thirteen soybean cultivars were exposed to a wide range of season-long elevated [O 3 ] in the field using free-air O 3 concentration enrichment. Elevated [O3] treatments reduced soybean seed yield from as little as 5.3% in 2005 to 35.2% in 2010. Although cultivars differed in yield response to elevated [O 3 ] (R), ranging from 17.5% to –76.4%, there was a significant negative correlation between R and O 3 dosage. Soybean cultivars showed greater seed yield losses to elevated [O 3 ] when grown at drier or hotter conditions compared to wetter or cooler years, because the hotter and drier conditions were associated with greater O 3 treatment. However, year-to-year variation in weather conditions did not influence the sensitivity of soybean seed yield to a given increase in [O 3 ]. Collectively, this study quantitatively demonstrates that, although drought conditions or warmer temperatures led to greater O 3 treatment concentrations and O 3 -induced seed yield reduction, drought and temperature stress did not alter soybean's sensitivity to O 3 . Our results have important implications for modeling the effects of rising O 3 pollution on crops and suggest that altering irrigation practices to mitigate O 3 stress may not be effective in reducing crop sensitivity to O 3 .

60 APPLIED LIFE SCIENCES↗

Incorporating Elevation in Traffic-Vehicle CO-Simulation: Issues, Impacts, and Solutions

Traffic-vehicle co-simulation couples microscopic traffic simulation with full-body vehicle dynamics to assess system-level impacts on mobility, energy, and safety with greater realism. Incorporating elevation is critical for accurately modeling vehicle behavior and energy use, especially for gradient-sensitive vehicles such as electric and heavy-duty trucks. However, raw elevation data often contain noise, discontinuities, and inconsistencies. While such issues may be negligible in traditional traffic simulations, they significantly affect traffic-vehicle co-simulations where vehicle dynamics are sensitive to road grade variations. This paper investigates the impact of unprocessed elevation data on vehicle behavior and energy consumption using a 42-mile simulation along Interstate 81. We propose an elevation processing workflow that can mitigate the effects stem from elevation data issues, improving the realism and stability of traffic-vehicle co-simulation. Results show that the method effectively removes noise and abrupt elevation transitions while preserving roadway geometry.

Xu, Guanhao [ORNL] (ORCID:0000000214326357)↗

Responses of tundra plant community carbon flux to experimental warming, dominant species removal and elevation

1. Rising temperatures can influence ecosystem processes both directly and indirectly, through effects on plant species and communities. An improved understanding of direct versus indirect effects of warming on ecosystem processes is needed for robust predictions of the impacts of climate change on terrestrial ecosystem carbon (C) dynamics. 2. To explore potential direct and indirect effects of warming on C dynamics in arctic tundra heath, we established a warming (open top chambers) and dominant plant species (Empetrum hermaphroditum Hagerup) removal experiment at a high and low elevation site. We measured the individual and interactive effects of warming, dominant species removal and elevation on plant species cover, the normalized difference vegetation index (NDVI), leaf area index (LAI), temperature, soil moisture and instantaneous net ecosystem CO 2 exchange. 3. We hypothesized that ecosystems would be stronger CO 2 sinks at the low elevation site, and that warming and species removal would weaken the CO 2 sink because warming should increase ecosystem respiration (ER) and species removal should reduce gross primary productivity (GPP). Furthermore, we hypothesized that warming and species removal would have the greatest impact on processes at the high elevation where site temperature should be most limiting and dominant species may buffer the overall community to environmental stress more compared to the low elevation site where plants are more likely to compete with the dominant species. 4. The instantaneous CO 2 flux, which reflected a weak CO 2 sink, was similar at both elevations. Neither experimental warming nor dominant species removal significantly changed GPP or instantaneous net ecosystem CO 2 exchange even though species removal significantly reduced ER, NDVI and LAI. 5. Our results show that even the loss of dominant plant species may not result in significant landscape-scale responses of net ecosystem CO 2 exchange to warming. They also show that NDVI and LAI may be limited in their ability to predict changes in GPP in these tundra heaths systems. Our study highlights the need for more detailed vegetation analyses and ground-truthed measurements in order to accurately predict direct and indirect impacts of climatic change on ecosystem C dynamics.

54 ENVIRONMENTAL SCIENCES↗

Photosynthetic capacity in middle-aged larch and spruce acclimates independently to experimental warming and elevated CO 2

Photosynthetic acclimation to both warming and elevated CO 2 of boreal trees remains a key uncertainty in modelling the response of photosynthesis to future climates. We investigated the impact of increased growth temperature and elevated CO 2 on photosynthetic capacity (V cmax and J max ) in mature trees of two North American boreal conifers, tamarack and black spruce. We show that V cmax and J max at a standard temperature of 25°C did not change with warming, while V cmax and J max at their thermal optima (T opt ) and growth temperature (T g ) increased. Moreover, V cmax and J max at either 25°C, T opt or T g decreased with elevated CO 2 . The J max /V cmax ratio decreased with warming when assessed at both T opt and T g but did not significantly vary at 25°C. The J max /V cmax increased with elevated CO 2 at either reference temperature. We found no significant interaction between warming and elevated CO 2 on all traits. If this lack of interaction between warming and elevated CO 2 on the V cmax , J max and J max /V cmax ratio is a general trend, it would have significant implications for improving photosynthesis representation in vegetation models. However, future research is required to investigate the widespread nature of this response in a larger number of species and biomes.

60 APPLIED LIFE SCIENCES↗

Data from: “Significant stream chemistry response to temperature variations in a high-elevation mountain watershed”

High-elevation mountain regions, central to global freshwater supply, are experiencing more rapid warming than low-elevation locations. High-elevation streams are therefore potentially critical indicators for earth system and water chemistry response to warming. Here we present concerted hydroclimatic and biogeochemical data from Coal Creek, Colorado in the central Rocky Mountains at elevations of 2700 to 3700 m, where air temperatures have increased by about 2 °C since 1980. We analyzed water chemistry every other day from 2016 to 2019. Water chemistry data indicate distinct responses of different solutes to inter-annual hydroclimatic variations. Specifically, the concentrations of solutes from rock weathering are stable inter-annually. Solutes that are active in soils, including dissolved organic carbon, vary dramatically, with double to triple peak concentrations occurring during snowmelt and in warm years. We advocate for consistent and persistent monitoring of high elevation streams to record early glimpse of earth surface response to warming.1) Coal Creek stream were sampled and measured to answer the question if there is a significant stream chemistry response to climate change in high-elevation watershed2) The file contains data regarding discharge, water temperature and stream chemistry data for 2016-2019 year. Stream chemistry includes dissolved organic and inorganic carbon, chloride, nutrients, cations. The data collection efforts were carried out by the DOE-funded "Advancing a Watershed Hydro-Biogeochemical Theory: Linking Water Travel Time and Reaction Rates Under Changing Climate" project and Watershed Function SFA.

54 ENVIRONMENTAL SCIENCES↗

Groundwater table elevation and temperature from 2015 to 2024 at the Lower Montane site in the East River Watershed, Colorado.

This groundwater level elevation and temperature data package is aimed at improving the predictive understanding of hydro-biogeochemical processes at the lower montane site in the East River Watershed, Colorado. The dataset is obtained using pressure transducers placed in shallow wells in the floodplain. This dataset contains data from wells with Location ID's ER-DOW (alias DO1West), ER-DOE (alias DO2East), ER-MBA1 (alias M1Bend1), ER-MBA2 (alias M1Bend2), ER-UPW (alias UP1West), ER-UPM (alias UP2), ER-UPE (alias UP3East). Another dataset contains the data from wells with Location ID's ER-CPA1 to ER-CPA6. Each file contains the water level elevation and the water temperature. Water level elevation has been obtained using the barometric pressure from the pressure transducer (Hobos sensor) in the well, barometric pressure from a sensor in air located at the same site (lower montane), depth from top-of-casing (TOC) to sensor measurement point, and TOC elevation. Data have been checked with a few measurements of water table depths. A real-time kinematic (RTK) global positioning system (GPS) has been used to survey the TOC (data in file Well_Location.csv). The water level elevation is given in UTM13N Geoid2012AB. While depth to water level is not present in the data files, it can be easily calculated with the TOC and distance to ground provided in the GPS coordinate file. The dataset quality is discussed in Collection/Analysis section of the methods. Time-series of measurements were initially added to the archive for the period 2015 to 2019, and later updated with time-series until 2024 (end of data collection). The dataset contains 8 *.csv data files, and 3 *.csv metadata files. Feel free to contact the author with any questions or collaboration interests. The publication year was updated from "2020" to "2025" to reflect the revised version of this dataset.

54 ENVIRONMENTAL SCIENCES↗

Groundwater table elevation and temperature from 2015 to 2024 across Meander C at the Lower Montane site in the East River Watershed, Colorado.

This groundwater level elevation and temperature data package is aimed at improving the predictive understanding of hydro-biogeochemical processes at the lower montane site in the East River Watershed, Colorado. The dataset is obtained using pressure transducers placed in shallow wells in the floodplain. This dataset contains data from wells ER-CPA1 to ER-CPA6. Another dataset contains the data from wells at nearby Locations. Each file contains the water level elevation and the water temperature. Water level elevation has been obtained using the barometric pressure from the pressure transducer (Hobos sensor) in the well, barometric pressure from a sensor in air located at the same site (lower montane), depth from top-of-casing (TOC) to sensor measurement point, and TOC elevation. Data have been checked with a few measurements of water table depths. A real-time kinematic (RTK) global positioning system (GPS) has been used to survey the TOC (data in file Well_Location.csv). The water level elevation is given in UTM13N Geoid2012AB. While depth to water level is not present in the data files, it can be easily calculated with the TOC and distance to ground provided in the GPS coordinate file. The dataset quality is discussed in Collection/Analysis section of the methods. Time-series of measurements were initially added to the archive for the period 2015 to 2019, and later updated with time-series until 2024 (end of data collection). The dataset contains 7 *.csv data files, and 3 *.csv metadata files. Feel free to contact the author with any questions or collaboration interests. The publication year was updated from "2020" to "2025" to reflect the revised version of this dataset.

54 ENVIRONMENTAL SCIENCES↗

The Elevated-Temperature Cyclic Properties of Alloy 316H Fabricated by Powder Metallurgy Hot Isostatic Pressing (Rev.1)

Powder metallurgy hot isostatic pressing (PM HIP), an advanced manufacturing technique, is viewed by the Department of Energy as a “transformational technology” for heavy section manufacturing of nuclear components. Although PM HIP is a mature technology, it has not been qualified in Section III, Division 5 for elevated-temperature nuclear construction. This work seeks to demonstrate a PM HIP qualification method for elevated-temperature nuclear construction that is based on allowable material characteristics. Specifically, the elevated-temperature cyclic properties of PM HIP 316H are investigated. The creep-fatigue properties of PM HIP 316H are significantly degraded compared to those of wrought. Current PM HIP practices are not able to fabricate elevated-temperature nuclear components with creep-fatigue properties that are equivalent to or better than traditional wrought components. A research and development program is proposed for qualifying PM HIP 316H for elevated-temperature nuclear construction.

36 MATERIALS SCIENCE↗

Elevated-temperature cyclic properties of advanced manufactured materials

Microreactor developers at the 2019 GAIN Microreactors workshop expressed a need for advanced manufacturing to fabricate microreactor components. Powder metallurgy (PM) hot isostatic pressing (HIP) is more mature than other advanced manufacturing techniques for the following reasons: 1. a code case exists for Section III, Division 1, Subsection NB components of light water reactors to be manufactured using PM HIP Alloy 316L, and 2. tensile and creep properties have been shown to be equivalent to wrought material for Type 316 stainless steel and Grade 91. Thus, PM HIP is more readily deployable in the near term. Elevated-temperature cyclic properties of PM-HIP materials, however, have not been established. The scope of testing for Code qualification could be significantly reduced compared to a new material for the following reasons: 1. only a limited amount of time consuming creep testing would be required, and 2. testing of fatigue and creep-fatigue behavior could be reduced if it can be demonstrated that the properties are comparable to wrought material. The objective of this work package is to develop an understanding of the relationship of advanced materials processing on the material characteristics as they relate to elevated-temperature component design and construction. This will be achieved by the following: • Elevated-temperature fatigue and creep-fatigue testing of PM HIP and wrought Alloy 316L. • Fracture and microstructural characterization of the PM HIP and wrought Alloy 316L. • Analysis of the elevated-temperature fatigue and creep-fatigue properties of PM HIP Alloy 316L. • Draft a report evaluating the elevated-temperature fatigue and creep-fatigue properties of PM HIP Alloy 316L. • Pursue procurement of Alloy 316H and Grade 91 manufactured using PM HIP. • Initiate scoping studies of the procured Alloy 316H or Grade 91 manufactured using PM HIP contingent upon successful material procurement.

36 MATERIALS SCIENCE↗

Significant stream chemistry response to temperature variations in a high-elevation mountain watershed

Abstract High-elevation mountain regions, central to global freshwater supply, are experiencing more rapid warming than low-elevation locations. High-elevation streams are therefore potentially critical indicators for earth system and water chemistry response to warming. Here we present concerted hydroclimatic and biogeochemical data from Coal Creek, Colorado in the central Rocky Mountains at elevations of 2700 to 3700 m, where air temperatures have increased by about 2 °C since 1980. We analyzed water chemistry every other day from 2016 to 2019. Water chemistry data indicate distinct responses of different solutes to inter-annual hydroclimatic variations. Specifically, the concentrations of solutes from rock weathering are stable inter-annually. Solutes that are active in soils, including dissolved organic carbon, vary dramatically, with double to triple peak concentrations occurring during snowmelt and in warm years. We advocate for consistent and persistent monitoring of high elevation streams to record early glimpse of earth surface response to warming.

54 ENVIRONMENTAL SCIENCES↗

Data from: "Ecophysiological variation in two provenances of Pinus flexilis seedlings across an elevation gradient from forest to alpine"

This archive contains data used to support conclusions drawn in “Ecophysiological variation in two provenances of Pinus flexilis seedlings across an elevation gradient from forest to alpine”, by Reinhardt et al., 2011. Data were collected over one summer season in plots within the Alpine Treeline Warming Experiment (ATWE), before climate manipulations began. The experiment was located on Niwot Ridge, in the Front Range of the Colorado Rocky Mountains. This data package includes five comma-separated-values (.csv) files, five Microsoft Excel (.xlsx) files, one .pdf file, and two types of geospatial files: keyhole markup language (.kml), and ESRI shapefiles (.shp). .csv files can be opened using any simple text-editing software (such as Notepad and TextEdit), R, and Microsoft Excel. .xlsx files can only be opened using Microsoft Excel. The .pdf file can be opened using Adobe Acrobat Reader or any other compatible file viewing software. The .kml file can be opened using Google Earth and Google Maps, and shapefiles can be opened using any software compatible with the file type, such as ESRI’s ArcGIS suite and QGIS.Data archived contain gas exchange and plant physiology measurements, non-structural carbohydrate data, among others. Geospatial files are also provided for additional locational context. The files and their contents in this data package are summarized under "Data Summary" in the included Data User's Guide. All files (excluding geospatial) are available in both Microsoft Excel and in .csv format, and are indicated in the Data Summary list as well.-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Climate change is predicted to cause upward shifts in forest tree distributions, which will require seedling recruitment beyond current forest boundaries. However, predicting the likelihood of successful plant establishment beyond current species’ ranges under changing climate is complicated by the interaction of genetic and environmental controls on seedling establishment. To determine how genetics and climate may interact to affect seedling establishment, we transplanted recently germinated seedlings from high- and low-elevation provenances (HI and LO, respectively) of Pinus flexilis in common gardens arrayed along an elevation and canopy gradient from subalpine forest into the alpine zone and examined differences in physiology and morphology between provenances and among sites. Plant dry mass, projected leaf area and shoot:root ratios were 12–40% greater in LO compared with HI seedlings at each elevation. There were no significant changes in these variables among sites except for decreased dry mass of LO seedlings in the alpine site. Photosynthesis, carbon balance (photosynthesis/respiration) and conductance increased >2× with elevation for both provenances, and were 35–77% greater in LO seedlings compared with HI seedlings. There were no differences in dark-adapted chlorophyll fluorescence (Fv/Fm) among sites or between provenances. Our results suggest that for P. flexilis seedlings, provenances selected for above-ground growth may outperform those selected for stress resistance in the absence of harsh climatic conditions, even well above the species’ range limits in the alpine zone. This indicates that forest genetics may be important to understanding and managing species’ range adjustments due to climate change.

54 ENVIRONMENTAL SCIENCES↗