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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 37 records · Page 2
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

An integrated multi-omics approach identifies the landscape of interferon-α-mediated responses of human pancreatic beta cells

Proinflammatory cytokines are important mediators of pancreatic beta cell dysfunction and demise in the early stages of type 1 diabetes (T1D). Interferon-a (IFNa), a type I interferon member, is expressed in the islets of T1D individuals and it is expression and signaling is regulated by both genetic (T1D risk variants) and environmental factors (viral infections) associated to T1D. We presently characterized human beta cells responses to IFNa by combining ATAC-seq, RNA-seq and proteomics assays. The initial beta cell response to IFNa was characterized by major chromatin remodeling, followed by marked changes in transcriptional and translational regulation. IFNa-induced changes in alternative splicing (AS) and first exon usage increased the diversity of transcripts expressed by beta cells. This, combined with changes observed on protein modification/degradation, ER stress and MHC class I, may significantly expand the peptide repertoire presented by beta cells to the immune system. On the other hand, beta cells up-regulated checkpoint proteins, such as PDL1 and HLA-E, that may protect them against the autoimmune assault. Data mining of the present multi-omics analysis led to the identification of two compound classes that revert IFNa effects on human beta cells and may be translated to clinical trials.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2024

Post-translational modifications: emerging directors of cell-fate decisions during endoplasmic reticulum stress in Arabidopsis thaliana

Homeostasis of the endoplasmic reticulum (ER) is critical for growth, development, and stress responses. Perturbations causing an imbalance in ER proteostasis lead to a potentially lethal condition known as ER stress. In ER stress situations, cell-fate decisions either activate pro-life pathways that reestablish homeostasis or initiate pro-death pathways to prevent further damage to the organism. Understanding the mechanisms underpinning cell-fate decisions in ER stress is critical for crop development and has the potential to enable translation of conserved components to ER stress-related diseases in metazoans. Post-translational modifications (PTMs) of proteins are emerging as key players in cell-fate decisions in situations of imbalanced ER proteostasis. In this review, we address PTMs orchestrating cell-fate decisions in ER stress in plants and provide evidence-based perspectives for where future studies may focus to identify additional PTMs involved in ER stress management.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

GDF15: a potential therapeutic target for type 1 diabetes

Type 1 Diabetes (T1D) is a pancreatic disease, which is marked by immune-mediated ? cell destruction causing hyperglycemic phenotype. Recently, our team has demonstrated the beneficial role of GDF15 in preventing T1D-associated insulitis. Therefore, in this review, we discuss the therapeutic potential of GDF15 in treating T1D and map the promising avenues demanding increased focus in this field. Areas covered: Several therapeutic options exist for management of T1D, however, the demand for new and efficient therapies persists, particularly for interventions that may prevent or delay disease onset. Growth differentiation factor 15 (GDF15) is a known diabetes biomarker which has shown impressive tissue-protective actions across a broad range of metabolic disorders. There is a tremendous amount of overlap between the two topics (GDF15 & T1D), however, the connections were uncharted. We discuss all these connections and the possibility of exploiting them to make GDF15 a T1D therapy. Expert Opinion: The newly discovered GDF15 primary receptor and associated signaling pathway has accelerated the excitement by making it a druggable target. However, the there exists a huge gap in knowledge regarding the mechanism by which GDF15 functions in the peripheral tissues. In this context, reports of GDF15 receptor in pancreas, gives a convincing argument of direct GDF15 effect. Further, GDF15’s role in regulating mitochondrial, ER and metabolic stress, makes it an ideal T1D candidate.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1997

Biweekly Maps of Wind Stress for the North Pacific from the ERS-1 Scatterometer

The European Remote-sensing Satellite (ERS-1) was launched in July 1991 and contained several instruments for observing the Earth's ocean including a wind scatterometer. The scatterometer measurements were processed by the European Space Agency (ESA) and the Jet Propulsion Laboratory (JPL). JPL reprocessed (Freilich and Dunbar, 1992) the ERS-1 backscatter measurements to produced a 'value added' data set that contained the ESA wind vector as well as a set of up to four ambiguities. These ambiguities were further processed using a maximum-likelihood estimation (MLE) and a median filter to produce a 'selected vector.' This report describes a technique developed to produce time-averaged wind field estimates with their expected errors using only scatterometer wind vectors. The processing described in this report involved extracting regions of interest from the data tapes, checking the quality and creating the wind field estimate. This analysis also includes the derivation of biweekly average wind vectors over the North Pacific Ocean at a resolution of 0.50 x 0.50. This was done with an optimal average algorithm temporally and an over-determined biharmonic spline spatially. There have been other attempts at creating gridded wind files from ERS-1 winds, e.g., kriging techniques (Bentamy et al., 1996) and successive corrections schemes (Tang and Liu, 1996). There are several inherent problems with the ERS-1 scatterometer. Since this is a multidisciplinary mission, the satellite is flown in different orbits optimized for each phase of the mission. The scatterometer also shares several sub-systems with the Synthetic Aperture Radar (SAR) and cannot be operated while the SAR is in operation. The scatterometer is also a single-sided instrument and only measures backscatter along the right side of the satellite. The processing described here generates biweekly wind maps during the wktwo years analysis period regardless of the satellite orbit or missing data.

SYNTHETIC APERTURE RADAR↗
DOE OSTI2023

MODFLOW6 models used to evaluate potential stresses and hydrologic conditions driving water-level fluctuations in well ER-5-3-2, Frenchman Flat, Southern Nevada

The hydrograph for well ER-5-3-2 in Frenchman Flat, southern Nevada, has previously unexplained water-level fluctuations. Four, three-dimensional, groundwater models (MODFLOW 6) were developed to evaluate potential stresses and hydrologic conditions affecting the well ER-5-3-2 hydrograph. Four model scenarios were developed that simulated: (1) wellbore leakage without recharge, (2) wellbore leakage with recharge, (3) shallow (low transmissivity) and deep (high transmissivity) carbonate rocks, and (4) lateral heterogeneity of carbonate rocks. Input and output files for the four model scenarios are in the model and output directories, respectively. Hydraulic conductivity, specific storage, and wellbore-leakage rates (when simulated) were estimated with parameter estimation (PEST) by minimizing a weighted composite, sum-of-squares objective function. The objective function was informed by measurement and Tikhonov regularization observations. Measurement observations included drawdowns from the constant-rate aquifer test and water-level altitudes measured in well ER-5-3-2 from 2001-2021. Tikhonov regularization informed hydraulic conductivity and specific storage parameters that were insensitive to measurement observations, where homogeneity was the preferred relation. Batch files, executables, and MODFLOW 6, PEST, and post-processing utilities are in the ancillary directory. Supplementary data also are included in the ancillary directory, including site information, high-frequency water-level and aquifer-test data, transmissivity estimates, water-chemistry data, and water-temperature analyses. This USGS data release contains data, analyses, and model files for the simulations and analysis results described in U.S. Geological Survey Scientific Investigations Report (https://doi.org/10.3133/sir20225132).

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1996

(abstract) Role of Long Equatorial Wave Reflection in the Low-Frequency Variability Observed During the TOPEX/POSEIDON Period: Data Analysis and Coupled Model Study

The analysis of TOPEX/POSEIDON sea level and ERS-1 zonal wind stress data gives evidence that first mode meridonal long Rossby waves fully reflected into Kelvin waves at the equatorial western Pacific boundary at different periods including the January-June 1994 period. The evolution of the conditions (zonal wind stress, sea surface temperature and sea level anomalies) in the central Pacific suggests that this reflection may have played a role in the reversal of cold to warm anomalies observed in the central Pacific in June-July 1994. To investigate the actual role of reflected Kelvin waves observed by TOPEX/POSEIDON, a simple ocean-atmosphere coupled model is designed. Starting from these initial conditions, several simulations are run for testing the role western bundary reflection evidenced in TOPEX/POSEIDON data. Coupled mechanisms are suggested to explain the low-frequency variability observed during this period.

Rossby waves Kelvin waves zonal wind stress TOPEX/↗
DOE OSTI2021

Revealing functional insights into ER proteostasis through proteomics and interactomics

The endoplasmic reticulum (ER), responsible for processing approximately one-third of the human proteome including most secreted and membrane proteins, plays a pivotal role in protein homeostasis (proteostasis). Dysregulation of ER proteostasis has been implicated in a number of disease states. As such, continued efforts are directed at elucidating mechanisms of ER protein quality control which are mediated by transient and dynamic protein-protein interactions with molecular chaperones, co-chaperones, protein folding and trafficking factors that take place in and around the ER. Technological advances in mass spectrometry have played a pivotal role in characterizing and understanding these protein-protein interactions that dictate protein quality control mechanisms. Here, we highlight the recent progress from mass spectrometry-based investigation of ER protein quality control in revealing the topological arrangement of the proteostasis network, stress response mechanisms that adjust the ER proteostasis capacity, and disease specific changes in proteostasis network engagement. We close by providing a brief outlook on underexplored areas of ER proteostasis where mass spectrometry is a tool uniquely primed to further expand our understanding of the regulation and coordination of protein quality control processes in diverse diseases.

60 APPLIED LIFE SCIENCES↗
NASA NTRS1993

An electrorheologically controlled semi-active landing gear

This study is to explore the application of electrorheology (ER) to the real-time control of damping forces that are transmitted through the nose landing gear for an F-106B aircraft. The main part of the landing gear is a strut that consists of a pneumatic spring and an ER controlled damper that is situatted on the strut centerline and applies a force directly opposing the vertical displacement of the nose wheel. The damping element rotates in response to strut displacement, employing a co-axial arrangement of stator and rotor plates connected to the opposing electrodes in the control circuit. The vertical displacement is conveted into rotation of the damper through a screw-nut mechanism. The ER fluid between the electrodes is thus engaged in shear along circumferential lines of action. This design results in a fast time response and a high ratio of strut forces achieved under Er-vs. zero-field control. Compact size and simplicity in fabrication are also attained. The analysis shows that when using an ER fluid of a yield stress of 7kPa, the energy absorption efficiency of the landing gear can reach almost 100% at various sink rates.

Lou, Zheng↗
NASA NTRS1999

Ice Thicknesses and Driving Stresses of Greenland Ice Sheet

Surface elevations from satellite radar altimetry (Geosat, Seasat, and ERS-1) and bedrock topography from airborne radar sounding (Simon Ekholm's Danish compilation) are combined to derive maps of the driving stresses in the Greenland ice sheet. The stress vector, tau = rho g h sin(alpha), is calculated using surface slope vectors, alpha, from surface elevations and ice thicknesses, h, from the difference between surface and basal elevations. Since the 5-km scale of the surface slope is only about 2 times the ice thickness, the stress maps show spatial variations indicative of longitudinal stress gradients associated with topographic undulations. Values of alpha generally vary from near zero at the ice divides to maxima values around 120 kpa, returning to near zero in a narrow band at the edges. The distribution of alpha's peaks at 60 kpa with an approximate sigma of +/- 20 kpa. Areas of very low alpha near the origin of the northeast ice stream may indicate small sub-glacial lakes. The profile of alpha, down the ice stream from near the ice divide, increases to a maximum of about 120 kpa near the margin, which is characteristic of East Antarctic outlet glaciers and in contrast to West Antarctic ice streams where alpha has maximum values 400 to 500 km inland from the grounding lines. Overall distributions of alpha values are compared with those for the Antarctic ice sheet and the Mars Northern ice cap.

Zwally, H. J.↗
NASA NTRS2014

Damage Characterization of EBC-SiCSiC Ceramic Matrix Composites Under Imposed Thermal Gradient Testing

Due to their high temperature capabilities, Ceramic Matrix Composite (CMC) components are being developed for use in hot-section aerospace engine applications. Harsh engine environments have led to the development of Environmental Barrier Coatings (EBCs) for silicon-based CMCs to further increase thermal and environmental capabilities. This study aims at understanding the damage mechanisms associated with these materials under simulated operating conditions. A high heat-flux laser testing rig capable of imposing large through-thickness thermal gradients by means of controlled laser beam heating and back-side air cooling is used. Tests are performed on uncoated composites, as well as CMC substrates that have been coated with state-of-the-art ceramic EBC systems. Results show that the use of the EBCs may help increase temperature capability and creep resistance by reducing the effects of stressed oxidation and environmental degradation. Also, the ability of electrical resistance (ER) and acoustic emission (AE) measurements to monitor material condition and damage state during high temperature testing is shown; suggesting their usefulness as a valuable health monitoring technique. Micromechanics models are used to describe the localized stress state of the composite system, which is utilized along with ER modeling concepts to develop an electromechanical model capable of characterizing material behavior.

protective coatings (nonmetallic)↗
NASA NTRS1995

Studies of radar backscatter as a function of wave properties and the winds in the turbulent marine atmosphere

The research on model functions for ADEOS and ERS-1 are summarized and an analysis of the differences between the three kinds of models is provided in this final report. The success of the AMI on ERS-1 obtained at GSFC and NMC is highlighted. The problem of wind stress description is reviewed within and the scatterometer model being developed for high winds monitoring for the AMI on ERS-1 and ERS-2 is described.

Pierson, Willard J., Jr.↗
NASA NTRS1996

(abstract) Ekman Pumping/Suction and Wind-Driven Ocean Circulation from ERS-1 Scatterometer Measurements Over the Arabian Sea During October 1994-October 1995

Spatial variations of the east-west and north-south components of surface wind stress are critical in studies of ocean circulation and biological-physical interactions because surface wind stress curl produces a vertical velocity in the upper ocean at the bottom of the Ekman Layer.The ERS-1 scatterometer provides reasonable coverage and direct measurements of vector of winds. Three schemes are evaluated relative to high-quality moored-bouy wind observations recorded in the central Arabian Sea, where high surface waves and high atmospheric water content during the southeast monsoon adversely affect the estimation of satellite-derived winds.

scatterometer↗
NASA NTRS1994

ERS-1 scatterometer measurements over the Southern Ocean

Backscatter cross section measurements from the ERS-1 Active Microwave Instrument (AMI) scatterometer were reprocessed to vector winds using the Freilich-Dunbar model function and a meteorologically aided ambiguity removal scheme. This consistent data set was used to examine the wind field over the Southern Ocean from 20 to 60 deg South. The large number of ERS-1 measurements allows relatively accurate calculation of annual mean wind, stress, and curl fields as well as overall statistics of the winds at mid to high southern latitudes. The long duration of the data time series allows preliminary examination of low frequency (semi annual) wind variability.

Freilich, M. H.↗
NASA NTRS2016

Temperature Dependence of Electrical Resistance of Woven Melt-Infiltrated SiCf/SiC Ceramic Matrix Composites

Recent studies have successfully shown the use of electrical resistance (ER)measurements to monitor room temperature damage accumulation in SiC fiber reinforced SiC matrix composites (SiCf/SiC) Ceramic Matrix Composites (CMCs). In order to determine the feasibility of resistance monitoring at elevated temperatures, the present work investigates the temperature dependent electrical response of various MI (Melt Infiltrated)-CVI (Chemical Vapor Infiltrated) SiC/SiC composites containing Hi-Nicalon Type S, Tyranno ZMI and SA reinforcing fibers. Test were conducted using a commercially available isothermal testing apparatus as well as a novel, laser-based heating approach developed to more accurately simulate thermomechanical testing of CMCs. Secondly, a post-test inspection technique is demonstrated to show the effect of high-temperature exposure on electrical properties. Analysis was performed to determine the respective contribution of the fiber and matrix to the overall composite conductivity at elevated temperatures. It was concluded that because the silicon-rich matrix material dominates the electrical response at high temperature, ER monitoring would continue to be a feasible method for monitoring stress dependent matrix cracking of melt-infiltrated SiC/SiC composites under high temperature mechanical testing conditions. Finally, the effect of thermal gradients generated during localized heating of tensile coupons on overall electrical response of the composite is determined.

Ceramic Matrix Composites↗
NASA NTRS2015

Correlation of Electrical Resistance to CMC Stress-Strain and Fracture Behavior Under High Heat-Flux Thermal and Stress Gradients

Because SiCSiC ceramic matrix composites (CMCs) are under consideration for use as turbine engine hot-section components in extreme environments, it becomes necessary to investigate their performance and damage morphologies under complex loading and environmental conditions. Monitoring of electrical resistance (ER) has been shown as an effective tool for detecting damage accumulation of woven melt-infiltrated SiCSiC CMCs. However, ER change under complicated thermo-mechanical loading is not well understood. In this study a systematic approach is taken to determine the capabilities of ER as a relevant non-destructive evaluation technique for high heat-flux testing, including thermal gradients and localized stress concentrations. Room temperature and high temperature, laser-based tensile tests were conducted in which stress-dependent damage locations were determined using modal acoustic emission (AE) monitoring and compared to full-field strain mapping using digital image correlation (DIC). This information is then compared with the results of in-situ ER monitoring, post-test ER inspection and fractography in order to correlate ER response to convoluted loading conditions and damage evolution.

electrical resistance↗
NASA NTRS1999

Assimilation of Satellite Altimeter Data With a Four Dimensional Model of the Japan Sea

A data assimilation is carried out to detect the variability of the Japan Sea circulation in the range from a few days to several years and from eddy to basin scale. The model applied in this study is the same 1/6 degree GFDL MOM1 as Kim and Yoon (1999) but is driven by ECMWF daily wind stress, heat and fresh water fluxes. The satellite altimeter data of TOPEX/POSEIDON, ERS-1 (phase C and G) and -2 are assimilated by an approximate Kalman filter (Fukumori and M.Rizzoli, 1995). The approximation is made by seeking an asymptotic steady error covariance matrix (Fukumori et al., 1993) and by introducing a coarser grid model for the innovation (data-model misfit). The coarse grid model is defined on 1/2 degree horizontal resolution and consists of the barotropic stream function, first baroclinic displacement and velocity amplitudes. The assimilated estimates explain about 6cm sea level variability of the data (approximately 12cm in the southern part), which is much larger than the previous reduced-gravity model and TOPEX/POSEIDON altimeter data assimilation (Hirose et al., 1999). The impacts of the T/P and ERS data on the filtered estimates are comparable. The result also shows high correlation to subsurface water temperatures measured by CTD. Many of the mesoscale eddies/disturbances travel east-northeastward with the advection speed of 1-3cm/s though most of them generated in the western region can not pass over the Oki Spur. The quasi-biennial variability found by Hirose and Ostrovskii (1999) did not show clear propagation pattern. The shallow Oki Spur may work as a "western boundary" to this signal. This is more plausible estimation than by the R-G model which has no bottom topography.

Hirose, Naoki↗