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At least 37 records · Page 2

AmeriFlux FLUXNET-1F US-xWR NEON Wind River Experimental Forest (WREF)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xWR NEON Wind River Experimental Forest (WREF). This is the FLUXNET version of the carbon flux data for the site US-xWR NEON Wind River Experimental Forest (WREF) produced by applying the standard ONEFlux (1F) software. Site Description - Located in an old growth Pacific Northwest forest west of the Cascade Range, Wind River Experimental Forest has a rich history of research and timber management since the early 1900s. The area is best known for its old-growth forests of Douglas-fir and western hemlock. Data collected at this site provides an interesting comparison to NEON’s ABBY site which is located Yacolt Burn State Forest, a relatively young growth industrial timber production forest.

Network), NEON (National Ecological Observatory [N↗

AmeriFlux FLUXNET-1F US-xRN NEON Oak Ridge National Lab (ORNL)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xRN NEON Oak Ridge National Lab (ORNL). This is the FLUXNET version of the carbon flux data for the site US-xRN NEON Oak Ridge National Lab (ORNL) produced by applying the standard ONEFlux (1F) software. Site Description - Oak Ridge National Laboratory (ORNL) is located at the U.S. Department of Energy's Oak Ridge Reservation in Roane County, Tennessee. The ORNL reservation is situated within the borders of five parallel ridges and valleys to the north of the Clinch River that are part of the Ridge-and-Valley Appalachians physiographic province (Environmental Sciences Division n.d.). The NEON tower site and Walker Branch aquatic site at ORNL are located within the Walker Branch Watershed, a 100 ha area that has served as the site for long-term environmental studies by the Environmental Sciences Division at ORNL, NOAA, and many visiting university researchers.

Network), NEON (National Ecological Observatory [N↗

AmeriFlux FLUXNET-1F PR-xLA NEON Lajas Experimental Station (LAJA)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site PR-xLA NEON Lajas Experimental Station (LAJA). This is the FLUXNET version of the carbon flux data for the site PR-xLA NEON Lajas Experimental Station (LAJA) produced by applying the standard ONEFlux (1F) software. Site Description - The NEON Lajas Experimental Station (LAJA) site is located on the southwest corner of the main island of Puerto Rico in a experimental range. This is a grassland site that is periodically grazed by cattle.

Network), NEON (National Ecological Observatory [N↗

AmeriFlux FLUXNET-1F US-xPU NEON Pu'u Maka'ala Natural Area Reserve (PUUM)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xPU NEON Pu'u Maka'ala Natural Area Reserve (PUUM). This is the FLUXNET version of the carbon flux data for the site US-xPU NEON Pu'u Maka'ala Natural Area Reserve (PUUM) produced by applying the standard ONEFlux (1F) software. Site Description - NEON's PUUM field site is located in the Pu'u Maka'ala Natural Area Reserve (NAR) on the eastern side of Hawaii’s “Big Island,” managed by the Hawaii Division of Forestry and Wildlife (DOFAW). More than 18,000 acres in size, the NAR is home to a rainforest with many native species, some of them endangered. It was established to protect some of the Big Island’s best wet native forest and unique geologic features.

Network), NEON (National Ecological Observatory [N↗

AmeriFlux FLUXNET-1F US-xLE NEON Lenoir Landing (LENO)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xLE NEON Lenoir Landing (LENO). This is the FLUXNET version of the carbon flux data for the site US-xLE NEON Lenoir Landing (LENO) produced by applying the standard ONEFlux (1F) software. Site Description - This terrestrial field site is located in a hardwood bottomland with seasonal flooding each spring located in southwest Alabama. The meteorological/flux tower and tower sampling plots are located at Lenoir Landing and the distributed plots are located approximately 5 km south of the tower at Choctaw National Wildlife Refuge. The ecosystem at LENO is dominated by closed-canopy pine-oak mixed forest with a developed understory, and also includes a small fraction of meadows, wetlands, and smaller water bodies. Dominant plant species include American sweetgum (Liquidambar styraciflua), American hornbeam (Carpinus caroliniana), and loblolly pine (Pinus taeda). Choctaw National Wildlife Refuge also provides a protected wintering area for waterfowl and wood duck brood habitat. This site is colocated with the Lower Tombigbee river aquatic site.

Network), NEON (National Ecological Observatory [N↗

AmeriFlux FLUXNET-1F US-xTE NEON Lower Teakettle (TEAK)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xTE NEON Lower Teakettle (TEAK). This is the FLUXNET version of the carbon flux data for the site US-xTE NEON Lower Teakettle (TEAK) produced by applying the standard ONEFlux (1F) software. Site Description - The site encompasses 5,138 hectares (12,696 acres) of mixed conifer and red fir forest, ranging in elevation from 1,990 to 2,807 m (6,529 – 9,209ft). The varied terrain is typical of the Sierra Nevada, with rugged mountains, meadows and prominent granite outcrops. TEAK has been designated as one of two relocatable terrestrial sites for the Pacific Southwest domain. The core aquatic site, Teakettle Creek, is just south of the terrestrial site.

Network), NEON (National Ecological Observatory [N↗

AmeriFlux FLUXNET-1F US-xSP NEON Soaproot Saddle (SOAP)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xSP NEON Soaproot Saddle (SOAP). This is the FLUXNET version of the carbon flux data for the site US-xSP NEON Soaproot Saddle (SOAP) produced by applying the standard ONEFlux (1F) software. Site Description - The Soaproot Saddle is a complex terrain of coarse hills, steep slopes and narrow drainages. With an elevation of 3274 - 4537’ this site encompasses 1438 acres of mixed conifer forests that are experiencing high levels of mortality due to native Pine beetles. At the core of this site stands a 171’ tall flux tower that collects physical and chemical properties of atmosphere and related process. Soaproot Saddle also hosts an array of sensor measurements along with field observations collected by highly trained NEON staff. The automated instrument measurements and some of the terrestrial observational safor this field site are colocted with NEON's aquatic site, Upper Big Creek, which is located just north of Soaproot Saddle's site boundaries.

Network), NEON (National Ecological Observatory [N↗

AmeriFlux FLUXNET-1F PR-xGU NEON Guanica Forest (GUAN)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site PR-xGU NEON Guanica Forest (GUAN). This is the FLUXNET version of the carbon flux data for the site PR-xGU NEON Guanica Forest (GUAN) produced by applying the standard ONEFlux (1F) software. Site Description - The Guanica Forest (GUAN) site is located on the southwest corner of the main island of Puerto Rico in a protected forest with primary phenological species of Gymnanthes lucida, Pisonia albida, and Bursera simaruba. The mean canopy height is approximately 10 m.

Network), NEON (National Ecological Observatory [N↗

Shrub and sedge rhizosphere communities display distinct affinities toward exudates and soil organic matter degradation: a quantitative stable isotope probing analysis

Warming temperatures are accelerating permafrost thaw and changing tundra vegetation, where woody shrubs are displacing sedges. Shrubs, such as Betula nana, and sedges, such as Eriophorum vaginatum, exhibit distinct life strategies including unique root-associated, or rhizosphere microbial communities. As permafrost thaws it unlocks previously unavailable carbon and nutrient sources resulting in deeper roots and a translocation of rhizosphere communities. Because permafrost microbial communities contain lower diversity and biomass than rhizosphere communities, the coalescence of rhizosphere and permafrost microbial communities could alter soil organic matter (SOM) degradation rates and increase greenhouse gas emissions. To identify metabolic strategies across distinct rhizosphere and permafrost microbial communities we conducted an isotope tracing incubation experiment. We inoculated thawed permafrost with shrub and sedge rhizosphere communities while adding exudates or water daily and compared this to an uninoculated control. After 46 days, we spiked samples with 18O enriched water or 13C enriched exudates and measured isotope incorporation into microbial DNA with quantitative stable isotope probing (qSIP). Our results indicate that exudate additions had little effect on uninoculated permafrost communities but the addition of exudates and rhizosphere inoculants had a compounding effect on respiration rates. We found that soils inoculated with shrub rhizosphere communities contained a mixture of exudate and SOM degraders while soils inoculated with sedge rhizosphere communities contained mainly SOM degraders. Finally, we found that individual microbial taxa exhibited maximum growth rates under specific combinations of microbial inoculant communities and exudate addition treatments. Our results reveal that microbial niches are strongly influenced by substrate preferences and community context, and suggest that a reduction in sedges and an expansion of shrubs may provide a mechanism by which permafrost carbon losses are mitigated through corresponding shifts in microbial communities and their substrate preferences.

Schaefer, Sean R [Natural Resources and Earth Syst↗

Drought increases microbial allocation to stress tolerance but with few tradeoffs among community-level traits

We leverage the Kellogg Biological Station Long Term Ecological Research Main Cropping System Experiment (KBS MCSE) field experiment to test trade-offs among high growth yield (Y), resource acquisition (A), and stress tolerance (S) traits using metagenomic data from plots with different land use, drought manipulation, and C addition.

Jones, Jennifer M. [WK Kellogg Biological Station,↗

Cis-regulatory evolution shapes facial diversity in birds and mammals

Birds and mammals exhibit extraordinary facial diversity, reflecting adaptations to distinct ecological niches and feeding strategies. While core face-building developmental programs are conserved and orchestrated by interactions between ectodermal organizers and the underlying mesenchyme, mechanisms driving facial shape variation remain poorly understood. Here, we integrate single-cell transcriptomic and chromatin accessibility profiling of mouse and chicken developing face to construct a comparative regulatory map. Although both ectodermal and mesenchymal populations display distinct regulatory features in each species, the mesenchyme exhibits markedly greater divergence, pointing to its central role in shaping facial morphology. We further reveal unexpected molecular complexity in the main face-shaping organizer, including a mouse-specific Shh/Wnt5a expression domain. At key morphogen loci (Bmp4, Fgf8, and Wnt5a), conserved and lineage-specific enhancers exhibit spatially restricted activity patterns that mirror divergent signaling domains. These findings demonstrate how cis-regulatory evolution modulates conserved developmental programs to generate morphological novelty, providing a valuable resource for studying vertebrate facial evolution.

Kyomen, Stella [Max Planck Institute for Evolution↗

Integrating terrestrial scavenging ecology into contemporary wildlife conservation and management

Scavenging plays a vital role in maintaining ecosystem health and contributing to ecological functions; however, research in this sub-discipline of ecology is underutilized in developing and implementing wildlife conservation and management strategies. We provide an examination of the literature and recommend priorities for research where improved understanding of scavenging dynamics can facilitate the development and refinement of applied wildlife conservation and management strategies. Due to the application of scavenging research broadly within ecology, scavenging studies should be implemented for informing management decisions. In particular, a more direct link should be established between scavenging dynamics and applied management programs related to informing pharmaceutical delivery and population control through bait uptake for scavenging species, prevention of unintentional poisoning of nontarget scavenging species, the epidemiological role that scavenging species play in disease dynamics, estimating wildlife mortalities, nutrient transfer facilitated by scavenging activity, and conservation of imperiled facultative scavenging species. This commentary is intended to provide information on the paucity of data in scavenging research and present recommendations for further studies that can inform decisions in wildlife conservation and management. Additionally, we provide a framework for decision making when determining how to apply scavenging ecology research for management practices and policies. Due to the implications that scavenging species have on ecosystem health, and their overall global decline as a result of anthropic activities, it is imperative to advance studies in the field of scavenging ecology that can inform applied conservation and management programs.

54 ENVIRONMENTAL SCIENCES↗

Mushroom body input connections form independently of sensory activity in Drosophila melanogaster

Associative brain centers, such as the insect mushroom body, need to represent sensory information in an efficient manner. In Drosophila melanogaster, the Kenyon cells of the mushroom body integrate inputs from a random set of olfactory projection neurons, but some projection neurons—namely those activated by a few ethologically meaningful odors—connect to Kenyon cells more frequently than others. This biased and random connectivity pattern is conceivably advantageous, as it enables the mushroom body to represent a large number of odors as unique activity patterns while prioritizing the representation of a few specific odors. How this connectivity pattern is established remains largely unknown. Here, we test whether the mechanisms patterning the connections between Kenyon cells and projection neurons depend on sensory activity or whether they are hardwired. We mapped a large number of mushroom body input connections in partially anosmic flies—flies lacking the obligate odorant co-receptor Orco—and in wild-type flies. Statistical analyses of these datasets reveal that the random and biased connectivity pattern observed between Kenyon cells and projection neurons forms normally in the absence of most olfactory sensory activity. Finally, this finding supports the idea that even comparatively subtle, population-level patterns of neuronal connectivity can be encoded by fixed genetic programs and are likely to be the result of evolved prioritization of ecologically and ethologically salient stimuli.

59 BASIC BIOLOGICAL SCIENCES↗

Effects of head-starting on multi-year space use and survival of an at-risk tortoise

A major challenge in the recovery of long-lived at-risk taxa like turtles is low juvenile recruitment. Head-starting—the raising of juveniles to larger sizes to improve survival—is one tool that can be used in circumstances where juvenile recruitment is limited. Due to declining populations and difficulty detecting juveniles, however, lack of knowledge of the ecology of juveniles can hinder efforts to develop and evaluate head-starting programs for many turtle species. We sought to inform recovery efforts of Mojave desert tortoises by quantifying multi-year space use and survival of head-started juveniles after release. We radio-tracked tortoises head-started under three different husbandry treatments that varied in rearing duration (from two to over six years) and whether head-starting included an indoor rearing component the first year. We compared postrelease space use and survival as a function of treatment, release size, and time since release. We found that space use, including home range size and site fidelity, varied by husbandry treatment, with smaller and younger tortoises having smaller home ranges and higher site fidelity. Additionally, home range size decreased and site fidelity increased with time since release across treatments. Tortoises with an indoor-rearing component experiencing increased risk of mortality as movement increased compared to tortoises reared solely outdoors. Nevertheless, survival did not differ among treatments or with tortoise age or size. Regardless of husbandry treatment, head-started tortoises exhibited similar space-use and survival overall. Our study provides insight into juvenile tortoise behavior and head-starting as a tool for tortoise conservation.

60 APPLIED LIFE SCIENCES↗

The transition from resistance to acceptance: Managing a marine invasive species in a changing world

Abstract Marine invasive species can transform coastal ecosystems, yet mitigating their effects can be difficult, and even impractical. Often, marine invasive species are managed at poorly matched spatial scales, and at the same time, rates of spread and establishment are increasing under climate change and can outpace resources available for population suppression. These circumstances challenge traditional conservation goals of maintaining a historic environmental state, especially for a species like the European green crab ( Carcinus maenas ), a formidable invader with few examples of successful long‐term removal programs. A management paradigm where decision alternatives include resisting or accepting a new ecological trajectory may be needed. We apply mathematical concepts from decision theory to develop a quantitative framework for navigating management decisions in this new resist‐accept paradigm. We develop a model of European green crab growth, removal and colonization, and we find optimal levels of removal effort that minimize both ecological change and removal cost. We establish a benchmark of colonization pressure at which green crab density becomes decoupled from a decision maker's actions, such that population control can no longer shape the invasion trajectory. For informing the decision boundary between resistance and acceptance, our results highlight that a decision maker's understanding of how removal cost scales with removal effort is more important than understanding the density‐impact relationship. We show that assuming stationary system dynamics can result in sub‐optimal levels of species removal effort, highlighting the importance of developing anticipatory management strategies by accounting for non‐stationary dynamics. Policy implications . For marine invasive species that can disperse across long distances and recolonize rapidly after removal, the focus of conservation policy should shift away from understanding how to resist change to understanding when to stop resisting change. Navigating this decision problem involves trade‐offs among competing objectives, highlighting the need for structured approaches to elicit objective weights that reflect the values of the decision maker. For natural resource managers facing possible ecosystem transformation, this decision framework can enable proactive and strategic decisions made under uncertainty in a changing world.

Keller, Abigail G. [Department of Environment Scie↗

Airborne LiDAR to Improve Canopy Fuels Mapping for Wildfire Modeling

Increasing conflict between wildfire and the built environment has increased the need for more up-to-date and finer resolution canopy fuels data to improve wildfire modeling and associated risk forecasts. The US Forest Service and US Department of the Interior’s LANDFIRE product, which provides 30-m resolution canopy fuels data for the entire US, is one of the most widely used sources of fuels data. However, the last complete mapping effort for LANDFIRE is based on 2016 conditions, and subsequent updates reflect disturbances 1-2 years behind the release year. Airborne systems equipped with Light Detection and Ranging (LiDAR) sensors can be deployed to actively sense canopy structure and estimate canopy fuels data (cover, height, base height, bulk density) at finer resolutions. Canopy base height (CBH) and canopy bulk density (CBD) are difficult to measure both in the field and in LiDAR point clouds. Still, they are important for accurately modeling crown fires, which are often intense and difficult to contain. Additionally, point cloud datasets are large, and calculations require efficient utilization of computational resources. To address these challenges, we are working on an approach that uses openly available National Ecological Observatory Network (NEON) airborne LiDAR data, with calculations processed in the R programming language and parallelized through the lidR package. CBH and CBD are often derived from tree height, diameter at breast height, and species-specific allometries using the Fire and Fuels Extension of the Forest Vegetation Simulator (FFE-FVS). We aim to test if airborne LiDAR can estimate CBH and CBD without the use of empirical equations. Reliable estimates of canopy fuels data directly from airborne LiDAR could streamline quick, fine-resolution updates for use in wildfire behavior models.

54 ENVIRONMENTAL SCIENCES↗

East Tennessee Technology Park Biological Monitoring and Abatement Program 2024 Calendar Year Report

The East Tennessee Technology Park (ETTP) Biological Monitoring and Abatement Program (BMAP) consists of three tasks that reflect different but complementary approaches to evaluating the ecological integrity of waters near ETTP. These tasks include (1) bioaccumulation monitoring of fish and clams, (2) benthic macroinvertebrate species richness and density monitoring, and (3) fish community monitoring. The sampling and analysis requirements for the ETTP BMAP in calendar year 2024, covering in part both FY 2024 and FY 2025, are outlined in the respective FY sampling and analysis plans (UCOR 2023, 2024). Sampled water bodies and locations for the ETTP BMAP are shown in Figures 1 and 2. This ETTP BMAP report presents the CY 2024 results and provides context with results from previous years. The report also includes Oak Ridge National Laboratory (ORNL)–generated biological monitoring data collected for other US Department of Energy programs, including the UCOR Water Resources Restoration Program (WRRP) off-site fish bioaccumulation data (UCOR 2023) and select Y-12 National Security Complex (Y-12) BMAP fish bioaccumulation data. Historical data collected for the ETTP BMAP and other programs in the nearby Poplar Creek and Clinch River are provided where appropriate. This progress report provides an update on the biological monitoring activities supporting the ETTP UCOR Environmental Compliance organization, which sponsors the ETTP BMAP. In addition to this internal reporting, ETTP BMAP results are provided in the annual remediation effectiveness reports and the annual site environmental reports, both of which are publicly available. BMAP data are also available to the public via the Oak Ridge Environmental Information System (https://ucor.com/oak-ridge-environmental-information-system-oreis/).

54 ENVIRONMENTAL SCIENCES↗

East Tennessee Technology Park Biological Monitoring and Abatement Program 2025 Calendar Year Report

The East Tennessee Technology Park (ETTP) Biological Monitoring and Abatement Program (BMAP) consists of three tasks that reflect different but complementary approaches to evaluating the ecological integrity of waters near ETTP. These tasks are (1) bioaccumulation monitoring of fish and clams, (2) benthic macroinvertebrate species richness and density monitoring, and (3) fish community monitoring.

54 ENVIRONMENTAL SCIENCES↗