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Sandia National Laboratories Annual Site Environmental Report, 2019: Tonopah Test Range, Nevada and Kaua'i Test Facility, Hawai'i

Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc., for the United States Department of Energy (DOE) National Nuclear Security Administration. The National Nuclear Security Administration’s Sandia Field Office administers the contract and oversees contractor operations at Sandia National Laboratories, Tonopah Test Range (SNL/TTR) in Nevada and Sandia National Laboratories, Kaua‘i Test Facility (SNL/KTF) in Hawai‘i. Activities at SNL/TTR are conducted in support of DOE weapons programs and have operated at the site since 1957. SNL/KTF has operated as a rocket preparation launching and tracking facility since 1962. DOE and its management and operating contractor are committed to safeguarding the environment, assessing sustainability practices, and ensuring the validity and accuracy of the monitoring data presented in this Annual Site Environmental Report. This report summarizes the environmental protection, restoration, and monitoring programs in place at SNL/TTR and SNL/KTF during calendar year 2019. Environmental topics include air quality, ecology, environmental restoration, oil storage, site sustainability, terrestrial surveillance, waste management, water quality, and implementation of the National Environmental Policy Act. This report is prepared in accordance with and as required by DOE O 231.1B, Admin Change 1, Environment, Safety, and Health Reporting, and has been approved for public distribution.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

2020 Annual Site Environmental Report for Sandia National Laboratories, Tonopah Test Range, Nevada, and Kaua'i Test Facility, Hawai'i

Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc., for the U.S. Department of Energy’s National Nuclear Security Administration. The National Nuclear Security Administration’s Sandia Field Office administers the contract and oversees contractor operations at Sandia National Laboratories, Tonopah Test Range (SNL/TTR) in Nevada and Sandia National Laboratories, Kaua‘i Test Facility (SNL/KTF) in Hawai‘i. Activities at SNL/TTR are conducted in support of U.S. Department of Energy weapons programs and have operated at the site since 1957. SNL/KTF has operated as a rocket preparation launching and tracking facility since 1962. The U.S. Department of Energy and its management and operating contractor are committed to safeguarding the environment, assessing sustainability practices, and ensuring the validity and accuracy of the monitoring data presented in this Annual Site Environmental Report. This report summarizes the environmental protection, restoration, and monitoring programs in place at SNL/TTR and SNL/KTF during calendar year 2020. Environmental topics include air quality, ecology, environmental restoration, oil storage, site sustainability, terrestrial surveillance, waste management, water quality, and implementation of the National Environmental Policy Act. This report is prepared in accordance with and as required by DOE O 231.1B, Admin Change 1, Environment, Safety and Health Reporting , and has been approved for public distribution.

54 ENVIRONMENTAL SCIENCES↗

Adoption of a token-based authentication model for the CMS Submission Infrastructure

The CMS Submission Infrastructure (SI) is the main computing resource provisioning system for CMS workloads. A number of HTCondor pools are employed to manage this infrastructure, which aggregates geographically distributed resources from the WLCG and other providers. Historically, the model of authentication among the diverse components of this infrastructure has relied on the Grid Security Infrastructure (GSI), based on identities and X509 certificates. In contrast, commonly used modern authentication standards are based on capabilities and tokens. The WLCG has identified this trend and aims at a transparent replacement of GSI for all its workload management, data transfer and storage access operations, to be completed during the current LHC Run 3. As part of this effort, and within the context of CMS computing, the Submission Infrastructure group is in the process of phasing out the GSI part of its authentication layers, in favor of IDTokens and Scitokens. The use of tokens is already well integrated into the HTCondor Software Suite, which has allowed us to fully migrate the authentication between internal components of SI. Additionally, recent versions of the HTCondor-CE support tokens as well, enabling CMS resource requests to Grid sites employing this CE technology to be granted by means of token exchange. After a rollout campaign to sites, successfully completed by the third quarter of 2022, the totality of HTCondor CEs in use by CMS are already receiving Scitoken-based pilot jobs. On the ARC CE side, a parallel campaign was launched to foster the adoption of the REST interface at CMS sites (required to enable token-based job submission via HTCondor-G), which is nearing completion as well. In this contribution, the newly adopted authentication model will be described. We will then report on the migration status and final steps towards complete GSI phase out in the CMS SI.

Pérez-Calero Yzquierdo, Antonio↗

University of Miami G-band Vapor Radiometer Calibration (UMGVR_CAL) Field Campaign Report

Cold-air outbreak (CAO) clouds in the Arctic are commonly mixed-phase (MP); however, the partitioning of the amount of ice and water in CAO clouds and precipitation is not always well observed. Understanding how cloud phases partition as a function of cloud life cycle is important for predicting snowfall rates, convective life cycle, and intensity at weather timescales. The partitioning into liquid versus ice also has radiative impacts that are consequential for climate. These concerns motivated the incorporation of an airborne G-band vapor radiometer (GVR) into an National Science Foundation-supported aircraft campaign named the Cold Air outbreak Experiment in the Sub-Arctic Region (CAESAR). The GVR is an upward-pointing passive microwave radiometer using four frequencies centered around the 183.31 GHz water vapor absorption line, displaced by +- 1, 3, 7, and 14 GHz. For context, The U.S. Department of Energy (DOE)’s Atmospheric Radiation Measurement (ARM) user facility operates a surface-based GVR at its North Slope of Alaska (NSA) site. The same GVR has been used previously for a field campaign in the southeast Pacific, where an offset was noticed between brightness temperatures (Tbs) measured under clear skies compared to those calculated from a radiative transfer model. To account for any calibration offsets to the Tbs, a request was made to DOE to allow the GVR to operate at ARM’s Southern Great Plains (SGP) observatory and enable comparisons between its measurements and those available at SGP. This request was granted, titled ‘UMGVR_CAL’, short for ‘UMGVR_Calibration’. From October 30 to November 10, 2023, the GVR was deployed to the ARM SGP site to take advantage of their regular, nearby radiosonde launches under clear-sky conditions. The latter were determined using the SGP total sky imagery data. The GVR brightness temperatures in these clear-sky conditions were compared to those calculated by a radiative transfer code (PAMTRA) based on the SGP radiosondes. During the campaign, four suitable clear-sky episodes could be used for the GVR calibration. While few in number, these proved to be enough to satisfy our goal.

54 ENVIRONMENTAL SCIENCES↗

NNSA-IAEC Science Area V Environmental ISR: Waste Management and Subsurface Science (Final Report FY18-FY20)

In August 2017, the U.S. National Nuclear Security Administration (NNSA) and the Israel Atomic Energy Commission (IAEC) launched a joint waste management program to evaluate the feasibility of siting a radioactive waste repository at intermediate depths in Israel. The bilateral collaboration involves three National Laboratories (LLNL, LANL, and Sandia), and the Nuclear Research Center, Negev, Geological Survey of Israel, and Ben Gurion University in Israel. This research is designed to assist with the evaluation of locations for an intermediate nuclear waste repository in Israel. This final report is a high-level summary of the results from the past three years by the team that worked on the WM-2: Radionuclide colloid-facilitated transport in fractured carbonate rock project for Science Area V, Environmental ISR: Subsurface Science and Waste Management. We have completed all of our tasks and met all of our milestones. MW-2 had three integrated tasks. Task 1 was laboratory experiments investigating colloid transport of radionuclides in natural fractured carbonate rocks from the Avdat formation, Israel. Task 2 was field experiments injecting radionuclide analogues pre-sorbed to clay colloids into fractured carbonate rock, and task 3 was numerical modeling of laboratory and field experimental results to assess the importance of colloids in the migration of relevant radionuclides. This task was incorporated into tasks 1 and 2. A 4 th task was initiated in FY20, to investigate the role of organics in facilitating radionuclide transport under the same conditions explored in task 1 due to the high organic content of the rocks being investigated for the immediate borehole locations. Preliminary results will be summarized here and the work will continue in FY21-23. The overall objective of this research is to evaluate the role of colloids (naturally occurring < 1 micron particles) in facilitating the transport of long-lived radionuclides from a nuclear waste repository situated in fractured carbonate rocks. Currently little data exists on radionuclide transport in carbonate rocks, but this is the main rock type available for siting a repository in Israel. Laboratory experiments were carried out in both Israel and the U.S., field experiments have taken place in Israel, and reactive transport modeling involved LLNL, LANL and Israel partner institutions. LLNL hosted a graduate student, Emily Tran, from Ben Gurion University in FY18 and FY19 and much of the work presented here was part of her PhD research.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

University of Miami G-band Vapor Radiometer Calibration at SGP during November 2023

From October 13, 2023 until November 16, 2023, the GVR was deployed to the DOE ARM SGP site, to take advantage of their regular near-by radiosonde launches under clear-sky conditions. The latter were determined using the SGP total sky imagery data. The GVR brightness temperatures in these clear-sky conditions were compared to those calculated by a radiative transfer code (PAMTRA) based on the SGP radiosondes. During the campaign, 4 suitable clear-sky episodes occurred that could be used for the GVR calibration. While few in number, these proved to be enough to satisfy our goal.

airborne G-band Vapor Radiometer↗

Kent Island Boundary Layer Field Campaign Report

Understanding the mechanisms governing the urban atmospheric environment is critical for informing urban populations about the impacts of climate change and associated mitigation and adaptation measures. The U.S. Department of Energy Atmospheric Radiation Measurement (ARM) User Facility’s Coast-Urban-Rural Atmospheric Gradient Experiment (CoURAGE) was conducted to study the interactions among the earth’s surface, the atmospheric boundary layer (ABL), aerosols, atmospheric composition, clouds, radiation, and precipitation at each experimental site, and to examine how the spatial gradients across the region interact to create the climate conditions in Baltimore. In this activity we performed weekly soundings, alternating between day and night from Kent Island, to get low-frequency, long-term observations of ABL/lower tropospheric thermodynamic structure. However, during the CoURAGE intensive operational period (IOP), we performed high-frequency sampling (2x/day), at times coinciding with CoURAGE IOP soundings at the main site. The short-term, high-frequency sounding information that was launched at 12Z and 18Z was to complement the longer-term data set.

54 ENVIRONMENTAL SCIENCES↗

The impact of r -process heating on the dynamics of neutron star merger accretion disc winds and their electromagnetic radiation

Neutron star merger accretion discs can launch neutron-rich winds of >10 -2 M⊙. This ejecta is a prime site for r-process nucleosynthesis, which will produce a range of radioactive heavy nuclei. The decay of these nuclei releases enough energy to accelerate portions of the wind by ~0.1c. Here, we investigate the effect of r-process heating on the dynamical evolution of disc winds. We extract the wind from a 3D general relativistic magnetohydrodynamic simulation of a disc from a post-merger system. This is used to create inner boundary conditions for 2D hydrodynamic simulations that continue the original 3D simulation. We perform two such simulations: one that includes the r-process heating, and another one that does not. We follow the hydrodynamic simulations until the winds reach homology (60 s). Using time-dependent multifrequency multidimensional Monte Carlo radiation transport simulations, we then calculate the kilonova light curves from the winds with and without dynamical r-process heating. We find that the r-process heating can substantially alter the velocity distribution of the wind, shifting the mass-weighted median velocity from 0.06c to 0.12c. The inclusion of the dynamical r-process heating makes the light curve brighter and bluer at $\sim 1\, \mathrm{d}$ post-merger. However, the high-velocity tail of the ejecta distribution and the early ($\lesssim 1\, \mathrm{d}$) light curves are largely unaffected.

79 ASTRONOMY AND ASTROPHYSICS↗

ARM Radiosondes for SNPP/JPSS Validation Field Campaign Report

This field campaign extension has been a coordinated effort involving the U.S. Department of Energy Atmospheric Radiation Measurement (ARM) User Facility, the University of Wisconsin at Madison, and the Joint Polar Satellite System (JPSS) project to validate NOAA Unique Combined Atmospheric Processing System (NUCAPS) temperature and moisture sounding products from the Cross-track Infrared Sounder (CrIS) and the Advanced Technology Microwave Sounder (ATMS) instruments on board the NOAA-20 and NOAA-21 satellite platforms. In this arrangement, funding for radiosondes and balloons was provided by the JPSS project to ARM. These radiosondes were launched coincident with NOAA-20 and NOAA-21 satellite overpasses at the ARM field sites at Eastern North Atlantic (ENA), North Slope Alaska (NSA), and Southern Great Plains (SGP). Combined with other ARM data, an assessment of the radiosonde data quality was performed and post-processing corrections applied. The dedicated radiosondes were integrated into the NOAA Products Validation System (NPROVS+), which collocated the radiosondes with satellite products (NOAA, National Aeronautics and Space Administration [NASA], European Organisation for the Exploitation of Meteorological Satellites [EUMETSAT], Geostationary Operational Environmental Satellite [GOES], Constellation Observing System for Meteorology, Ionosphere, and Climate [COSMIC]) and numerical weather prediction (NWP) forecasts for use in product assessment and algorithm development. This work is a part of the NOAA-20 and NOAA-21 satellite retrieval validation efforts and provides critical accuracy assessments of the temperature and water vapor soundings.

54 ENVIRONMENTAL SCIENCES↗

A Search for Millimeter-bright Blazars as Astrophysical Neutrino Sources

The powerful jets of blazars have been historically considered as likely sites of high-energy cosmic-ray acceleration. However, the particulars of the launched jet and the locations of leptonic and hadronic jet loading remain unclear. In the case when leptonic and hadronic particle injection occur jointly, a temporal correlation between synchrotron radiation and neutrino production is expected. We use a first catalog of millimeter wavelength (95–225 GHz) blazar light curves from the Atacama Cosmology Telescope for a time-dependent correlation with 12 yr of muon neutrino events from the IceCube South Pole Neutrino Observatory. Such millimeter emission traces activity of the bright jet base, which is often self-absorbed at lower frequencies and potentially gamma-ray opaque. We perform an analysis of the population, as well as analyses of individual, selected sources. We do not observe a significant signal from the stacked population. TXS 0506+056 is found as the most significant, individual source, though this detection is not globally significant in our analysis of selected active galactic nuclei. Our results suggest that the majority of millimeter-bright blazars are neutrino dim. In general, it is possible that many blazars have lighter, leptonic jets, or that only selected blazars provide exceptional conditions for neutrino production.

Abbasi, R. [Loyola University, Chicago, IL (United↗

Optimizing Energy Use in Pulp & Paper with DOE’s Energy Intensive Industries Resources

The U.S. pulp and paper industry is the third-largest energy consumer in manufacturing, accounting for roughly 10% of sector energy use. Improving energy efficiency reduces operating costs and strengthens competitiveness. To support this effort, the U.S. Department of Energy (DOE), through Oak Ridge National Laboratory (ORNL), launched the Energy Intensive Industries (EII) Initiative. A two-year pilot across 45 industrial sites identified more than 4 trillion Btu/year in potential energy savings. This presentation outlines plans for a follow-up technical assistance program tailored to pulp and paper mills. Available resources include a cost-savings scoping tool, implementation planning guidance, and technical support for applying advanced methods such as Pinch Analysis for integrated process-utilities optimization. The session introduces key Pinch Analysis principles and highlights case studies demonstrating measurable improvements. ORNL also seeks industry feedback on barriers to efficiency improvements, including technology gaps and resource needs. DOE’s broader objective is to accelerate productivity and economic competitiveness across U.S. energy-intensive industries.

Kamath, Dipti [ORNL] (ORCID:0000000278739994)↗

Oak Ridge National Laboratory FY 2023 Site Sustainability Plan With FY 2022 Performance Data

At the close of each fiscal year, the US Department of Energy (DOE) Sustainability Performance Division (SPD) issues guidance documents and technical resource aids/tools necessary for DOE sites and national laboratories to complete sustainability reporting requirements. SPD is part of the DOE Office of Asset Management. As required by DOE Order 436.1, Departmental Sustainability, “each site will develop and commit to an annual Site Sustainability Plan (SSP) that identifies its respective contribution toward meeting the DOE’s sustainability goals.” SPD collects and compiles information reported by each site to develop an agency-wide Sustainability Report and Implementation Plan, which is used to report DOE sustainability progress to the federal government as required by all major federal agencies. DOE launched a formal Sustainability Office and annual SSP process in 2011. Each year, Oak Ridge National Laboratory (ORNL), in concert with the Office of Science (SC), provides the resources essential to fulfill its commitment to deliver a complete and accurate SSP report and quality performance data for entry into the DOE Sustainability Dashboard as managed by SPD. The performance data entered by each DOE site are then combined to disclose the progress of each DOE Program Office and are further combined to show comprehensive progress for the agency. The Office of Asset Management provides assistance to program offices in sustaining their missions, freeing up resources by reducing waste, avoiding excess expenditure on utilities, maximizing productivity, and improving the efficiency of facilities and processes. By focusing on mission needs, programs and associated DOE sites can help the agency meet its sustainability goals, as outlined in federal statutory and regulatory requirements. In FY 2022, the SSP guidance was updated to capture requirements from Executive Order (EO) 14008, Tackling the Climate Crisis at Home and Abroad, the Energy Act of 2020 (EAct 20), actions outlined in DOE’s Climate Adaptation & Resilience Plan and Sustainability Plan, and EO 14057, Catalyzing Clean Energy Industries and Jobs Through Federal Sustainability. Updates in SSP guidance help to minimize and streamline reporting while simultaneously addressing updated federal requirements. Per DOE, each SSP report should provide an overview of the site’s planned actions, as well as an overview of efforts and accomplishments during the reporting period. SPD collects and compiles information reported by each site to develop DOE’s Annual Sustainability Report, Climate Adaptation & Resilience Plan, and Annual Energy Management Report to Congress. The agency goal has been to lower the reporting burden for sites and increase and improve the consistency of information available to decision makers, allowing them to better identify projects and potential for increased efficiency, as well as to reduce waste, lower emissions, and enhance operational resilience. Sites may elect to produce a more polished publication for their leadership and stakeholders, but this step is no longer required. The ORNL SSP narrative report (this document) and the reporting of DOE SPD Sustainability Dashboard performance data is a collaborative effort of approximately 30 subject matter experts (SMEs) from ORNL facility management and research divisions. Annually, these associates come together to provide a report that can be used by DOE to demonstrate continued agency progress in energy efficiency and sustainable federal operations.

99 GENERAL AND MISCELLANEOUS↗

Collapsar R-process Yields Can Reproduce [Eu/Fe] Abundance Scatter in Metal-poor Stars

It is unclear if neutron star mergers can explain the observed r-process abundances of metal-poor stars. Collapsars, defined here as rotating massive stars whose collapse results in a rapidly accreting disk around a black hole that can launch jets, are a promising alternative. We find that we can produce a self-consistent model in which a population of collapsars with stochastic europium yields synthesizes all of the r-process material in metal-poor ([Fe/H] < - 2.5) stars. Our model reproduces the observed scatter and evolution of scatter of [Eu/Fe] abundances. We find that if collapsars are the dominant r-process site for metal-poor stars, r-process synthesis may be linked to supernovae that produce long γ-ray bursts. Our results also allow for the possibility that core-collapse supernovae beyond those that launch γ-ray bursts also produce r-process material (e.g., potentially a subset of Type Ic-BL supernovae). Furthermore, we identify collapsar jet properties (isotropic energy, engine luminosity, or engine time) that may trace r-process yield and verify that the amount of r-process yield produced per collapsar in our model ( ~ 0.07M⊙) is consistent with other independent estimates. In the future, achieving 0.05 dex precision on distribution scatter or a reliable selection function would further constrain our probe of r-process production. Our model would also hold for another prompt r-process site with a power-law yield, and work is needed to determine if, for example, fast-merging neutron stars can also explain abundance scatter.

Astronomy & Astrophysics↗

A New State of the Art: Zero Energy Modular Multifamily Construction

In 2020, the U.S. Department of Energy's Building Technologies Office (BTO) launched the Advanced Building Construction (ABC) Initiative. This initiative aimed to integrate energy efficiency into high-production construction practices. One focus area of the initiative is off-site construction, an approach that may achieve scalable, efficient, and high-performing construction buildings through process standardization. This report presents the findings and recommendations from our ABC Initiative project: A New State of the Art: Zero-Energy Modular Multifamily Construction System. The objectives of the BTO-funded project are to: 1) Achieve energy performance 50% better than the 2018 International Energy Conservation Code (IECC). 2) Achieve this level of energy performance at no additional cost VEIC worked with Louisiana State University (LSU), National Renewal Energy Lab (NREL), [the project team], and industry partners at the Mod Coach, KBS, and Solar Home Factory to identify and analyze multifamily business designs and modular construction practices. These designs and processes hold the promise of achieving the goals set out by the DOE.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Accelerating Nuclear-Integrated Data Centers in the USA: SWOT Analysis, Power-Thermal Management Strategies, and Industrial-Scale Demonstration and Potential Deployment

Driven by the growth in digital services, cloud computing, AI, and manufacturing, data centers face rising energy demands that challenge traditional power sources and cooling efficiency. This study explores using nuclear power to meet these demands, focusing on accelerated reactor technology deployment and highlighting needs such as N+1/N+2 power supplies and integrated power-thermal management. A SWOT analysis addresses grid connectivity, reactors, and site selection, particularly DOE sites. Reactor technology demonstration and deployment could be accelerated by leveraging test facilities such as MARVEL, MAGNET, TED, FAS, DOME, LOTUS, ATR, Energy System Proving Grounds, and upcoming Energy Launch Pads, along with modeling and simulation tools such as RELAP5, MOOSE, VERA, RAVEN, and FORCE. The potential power and thermal management options, including various cooling technologies, waste-heat utilization, and an industrial-scale demonstration plan, aim to accelerate the integration of nuclear power and data centers in the USA, while emphasizing community and stakeholder engagement and synergistic efforts.

21 - SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLAN↗

On the Road to Achieving Safety Excellence at the Idaho Cleanup Project: The Initial Challenges, Solutions, and Positive Results of Striving to be the Best and Safest in the DOE Complex - 20518

When Fluor Idaho, LLC (Fluor Idaho) assumed the Idaho Cleanup Project Core contract (ICP Core) in mid-2016, it inherited the successful multi-faceted, employee-owned safety programs of two prior contractors supporting cleanup operations at the Idaho National Laboratory Site. Fluor Idaho incorporated the best of both safety programs along with Fluor Corporate's 106-year legacy of safety initiatives and successes. Fluor Idaho integrated the separate programs and cultures under a One Fluor approach - one project, one mission, One Fluor. Job changes, management changes, and procedures, processes, and system changes presented multiple challenges. Those challenges combined with the severe Winter of 2016/2017 resulted in numerous slips, trips, and falls first aids and reportable injuries. The road to safety excellence started off bumpy and stayed that way for some time. Since then, Fluor Idaho has successfully implemented several key initiatives including employee engagement; management engagement and time in the field; communications improvements; safety culture sustainment; injury/illness prevention; organizational learning; and DOE Voluntary Protection Program (VPP) readiness and preparation. Management became more engaged by making more workplace visits and increasing their supervision of 'life critical work' or complex activities. Additional training became mandatory for first-line supervisors and front-line workers. Most recently, Fluor Idaho increased its injury and illness prevention initiatives extensively. In the last year, things have turned around in a dramatic way. This shift is directly attributable to the significant number of initiatives launched in late 2017 and 2018. These initiatives addressed safety performance in the five key areas of the DOE VPP and promoted sustainability of the company's safety culture. The five key areas are: management leadership, employee involvement, work-site analysis, hazard prevention and control, and safety and health training. Employees and managers alike recommitted to making safety the highest value. The result of this laser-like focus on safety and accident and injury prevention is that injuries and first aids are a fraction of what they were. During FY 2019, employees worked more than 4.2 million hours without a serious injury or lost work-day and 1 million hours without a recordable injury. Fluor Idaho is proud of its safety trend. The company invested significant resources in improving its safety record, and that investment is paying off. Other contractors in the DOE Complex can learn and benefit from this discussion of the challenges Fluor Idaho faced, as well as its innovative safety initiatives that are producing results that are being recognized by our U.S. Department of Energy (DOE) customer. (authors)

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Perspectives on ENCODE

The Encyclopedia of DNA Elements (ENCODE) Project launched in 2003 with the long-term goal of developing a comprehensive map of functional elements in the human genome. These included genes, biochemical regions associated with gene regulation (for example, transcription factor binding sites, open chromatin, and histone marks) and transcript isoforms. The marks serve as sites for candidate cis-regulatory elements (cCREs) that may serve functional roles in regulating gene expression. The project has been extended to model organisms, particularly the mouse. Finally, in the third phase of ENCODE, nearly a million and more than 300,000 cCRE annotations have been generated for human and mouse, respectively, and these have provided a valuable resource for the scientific community.

59 BASIC BIOLOGICAL SCIENCES↗