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Estimating Individual Tree Mortality in the Sierra Nevada Using Lidar and Multispectral Reflectance Data

Widespread tree mortality events occur during periods of severe drought in temperate conifer forests and are expected to become more frequent in many areas due to climate change. Improved mapping of individual tree mortality is needed to identify risk factors and design effective conservation strategies. In this study, we used National Ecological Observatory Network (NEON) lidar and multispectral reflectance airborne observations to map individual tree mortality over a 160 km 2 area during and after the 2012–2016 drought for two sites in California's Sierra National Forest. We used NEON lidar to derive tree locations and crown perimeters and multispectral data to map tree mortality for more than 1 million trees. We found that 25.4% of the trees in our study area died between 2013 and 2017, with considerably higher mortality at the lower-elevation Soaproot Saddle site. Between 2017 and 2019, an additional 2.0%–2.8% of the trees died each year. Two wildfires in 2020 and 2021 increased tree mortality within burned area perimeters by 49%–89% between 2019 and 2021. Consistent with previous work, we found that tree mortality risk increased as a function of tree height. Tree mortality was positively associated with distance from rivers, trees per hectare, and decreasing slope at the lower elevation site. In contrast, increasing slope was positively associated with tree mortality at the higher elevation site. Our approach and dataset provide a means to study the combined effects of drought and wildfire on tree mortality and may improve projections of forest resilience under a changing climate.

54 ENVIRONMENTAL SCIENCES↗

Southeast Ecological Observatory Network (SEEON) Workshop on Ecological Sensors and Information Technology. Report on Second SEEON Workshop

A fundamental goal of the new National Science Foundation (NSF) initiative National Ecological Observatory Network (NEON) is to provide timely and broad access to the ecological data collected at NEON sites. Information management and data collection will be critical components to achieving this goal and a successful NEON implementation. The Southeast Ecological Observatory Network (SEEON) working group recognized the importance of information management and sensor technology in its first planning workshop and recommended that interested parties in the region come together to discuss these subjects in the context of the needs and capabilities of a southeast regional ecological observatory network. In February 2004, 28 participants from 14 organizations including academic institutions, state and federal agencies, private and non-profit entities convened at the Space Life Sciences Laboratory (SLSL) at the Kennedy Space Center, Florida for two days of presentations and discussions on ecological sensors and information management. Some of the participants were previously involved in the first SEEON workshop or other meetings concerned with NEON, but many were somewhat new to the NEON community. Each day focused on a different technical component, i.e. ecological sensors the first day and cyber-infrastructure the second day, and were structured in a similar manner. The mornings were devoted to presentations by experts to help stimulate discussions on aspects of the focal topic held in the afternoon. The formal and informal discussions held during the workshop succeeded in validating some concerns and needs identified in the first SEEON workshop, but also served to bring to light other questions or issues that will need to be addressed as the NEON planning and design stages move forward. While the expansion of the SEEON community meant that some of the presentation and discussion time was needed to help bring the newcomers up to speed on the goals, objectives and current status of the various NEON efforts, the additional perspectives and technical expertise included in this workshop helped fuel some valuable interdisciplinary discussions that will need to continue to bring SEEON and NEON to fruition. Participants agreed that continued discussions of SEEON are needed , to keep up the momentum and that the southeast region must continue to be represented at the national level. It is vital that the all'the regions continue to push things forward for NEON to succeed.

Jones, Lori N.↗

2024 Workshop - Remote Sensing and Fluxes Upscaling for Real-world Impact - Tutorial v1

The software-tutorial was developed within the 2024 Remote Sensing and Fluxes Upscaling for Real-world Impact workshop as part of the hands-on session. The workshop was supported by AmeriFlux, National Ecological Observatory Network (NEON) and CarbonDew. The software provides basic tools to perform the following tasks: - gather remote sensing images using Google Earth Engine API; - gather flux data; - perform basic functions, such as plotting time-series, perform QA of the data, compute vegetation indices; - perform correlation analysis between flux data and remote sensing data; - perform flux predictions based on remote sensing data integrated in different modalities.

Falco, Nicola [Lawrence Berkeley National Laborato↗

Recommendations for developing, documenting, and distributing data products derived from NEON data

The National Ecological Observatory Network (NEON) provides over 180 distinct data products from 81 sites (47 terrestrial and 34 freshwater aquatic sites) within the United States and Puerto Rico. These data products include both field and remote sensing data collected using standardized protocols and sampling schema, with centralized quality assurance and quality control (QA/QC) provided by NEON staff. Such breadth of data creates opportunities for the research community to extend basic and applied research while also extending the impact and reach of NEON data through the creation of derived data products—higher level data products derived by the user community from NEON data. Derived data products are curated, documented, reproducibly-generated datasets created by applying various processing steps to one or more lower level data products—including interpolation, extrapolation, integration, statistical analysis, modeling, or transformations. Derived data products directly benefit the research community and increase the impact of NEON data by broadening the size and diversity of the user base, decreasing the time and effort needed for working with NEON data, providing primary research foci through the development via the derivation process, and helping users address multidisciplinary questions. Creating derived data products also promotes personal career advancement to those involved through publications, citations, and future grant proposals. However, the creation of derived data products is a nontrivial task. Here we provide an overview of the process of creating derived data products while outlining the advantages, challenges, and major considerations.

54 ENVIRONMENTAL SCIENCES↗

NEON AOP Survey of Upper East River CO Watersheds: Waveform LiDAR Binary Data

The waveform Light Detection and Ranging (LiDAR) data in this package were generated through a National Ecological Observatory Network Airborne Observation Platform (NEON AOP) acquisition over watersheds of interest surrounding Crested Butte, Colorado. The remote sensing imagery acquired by the NEON AOP supports an interdisciplinary project on hydrology, biogeochemistry, and ecosystem functioning in a snow-dominated headwater environment. These waveform LiDAR data enable spatially continuous estimation of vegetation structure parameters to facilitate analyses of the major environmental drivers of structural and compositional variability. The package contains 97 compressed file archives in 7-zip (.7z) format, each corresponding to one acquisition flightpath. Within each .7z archive is a set of constituent files describing properties of the LiDAR waveforms, such as return intensity, geolocation, outgoing pulse and other behavior of the sensor and signals. Once downloaded, the files must first be unzipped using the widely distributed command-line software utility 7z, using the command '7z x \[filename\].7z \[target_directory\]'. All files within the .7z archives can be opened in IDL, MatLab, or the open-source R statistical computing environment. Further details about the data package are in the attached user guide (neon_aop_crbu_waveformlidar_userguide.pdf). This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS Campaigns↗

Key predictors of soil organic matter vulnerability to mineralization differ with depth at a continental scale

Abstract Soil organic matter (SOM) is the largest terrestrial pool of organic carbon, and potential carbon-climate feedbacks involving SOM decomposition could exacerbate anthropogenic climate change. However, our understanding of the controls on SOM mineralization is still incomplete, and as such, our ability to predict carbon-climate feedbacks is limited. To improve our understanding of controls on SOM decomposition, A and upper B horizon soil samples from 26 National Ecological Observatory Network (NEON) sites spanning the conterminous U.S. were incubated for 52 weeks under conditions representing site-specific mean summer temperature and sample-specific field capacity (−33 kPa) water potential. Cumulative carbon dioxide respired was periodically measured and normalized by soil organic C content to calculate cumulative specific respiration (CSR), a metric of SOM vulnerability to mineralization. The Boruta algorithm, a feature selection algorithm, was used to select important predictors of CSR from 159 variables. A diverse suite of predictors was selected (12 for A horizons, 7 for B horizons) with predictors falling into three categories corresponding to SOM chemistry, reactive Fe and Al phases, and site moisture availability. The relationship between SOM chemistry predictors and CSR was complex, while sites that had greater concentrations of reactive Fe and Al phases or were wetter had lower CSR. Only three predictors were selected for both horizon types, suggesting dominant controls on SOM decomposition differ by horizon. Our findings contribute to the emerging consensus that a broad array of controls regulates SOM decomposition at large scales and highlight the need to consider changing controls with depth.

59 BASIC BIOLOGICAL SCIENCES↗

Wind River Experimental Forest Subcanopy Tower Information Sheet

Wind River was one of three sites that collected 3d sonic anemometer data for an ICOS subcanopy observation study. The three sites were defined by the following features and terrain: a deciduous broadleaf forest in flat terrain (Lanžhot, Czech Republic), a coniferous forest in mountainous terrain (Renon, Italy), and a tall conifer forest in mountain-valley terrain (Wind River, USA). The Wind River subcanopy towers were deployed in a high LAI, old-growth evergreen conifer forest and collected approximately 11 months of data. The site is an ecologically rich temperate rainforest in the western Cascade Mountains, and the biological carbon sink and source strength has been measured since 1998 using eddy covariance on the top of a 74 m tall flux tower (currently called the Wind River NEON tower). Additionally, forest inventory records date back to the 1920s. In 2024, four subcanopy towers were installed near the Wind River NEON tower to measure wind flow in the understory canopy layer for better understanding canopy flow coupling and decoupling in the subcanopy and how this affects the interpretation of overstory fluxes. The subcanopy tower installation was done by Lawrence Livermore National Laboratory and Washington State University (WSU) with collaborations from the University of Utah and the National Ecological Observatory Network (NEON).

54 ENVIRONMENTAL SCIENCES↗

Community stability is related to animal diversity change

Abstract Understanding the drivers of community stability in times of increasing anthropogenic pressure is an urgent issue. Biodiversity is known to promote community stability, but studies of the biodiversity–stability relationship rarely consider the full complexity of biodiversity change. Furthermore, finding generalities that hold across taxonomic groups and spatial and temporal scales remains challenging because most investigations have narrow taxonomic, spatial, and temporal scopes. We used organismal data collected through the National Ecological Observatory Network (NEON) at sites across the contiguous United States to evaluate linkages between community stability and biodiversity change for four taxonomic groups: small mammals, ground beetles, fish, and freshwater macroinvertebrates. We defined community stability as constancy of aggregate species' abundance. We quantified change in biodiversity as (1) dissimilarity in community taxonomic and functional composition and species replacement and richness change components of that dissimilarity and (2) change in species' abundance distributions as captured by change in species rank, richness, and evenness. We found that community stability increased with species replacement and with contribution of species replacement to overall dissimilarity for all taxonomic groups, but declined with increasing change in species richness and evenness. This is consistent with the notion that temporal fluctuations in species abundance can help stabilize community properties. We also found that community stability was highest when change in community functional composition was either lower or higher than expected given reshuffling of each community's taxonomic composition. This suggests that long‐term community stability can result from fluctuations of functionally similar species in assemblages with high taxonomic reshuffling. On the contrary, the functional uniqueness of fluctuating species compensates for lower taxonomic reshuffling to drive stabilization of community properties. Our study provides an initial assessment of the relationship between community stability and biodiversity change and illustrates the utility of fine temporal resolution data collected across ecosystems and biomes to understand the general mechanisms underlying biodiversity–stability relationships.

54 ENVIRONMENTAL SCIENCES↗

Daily evapotranspiration changes during heatwaves at 32 NEON sites, 2019-2021

This dataset provides partitioned evapotranspiration (ET, the combined loss of water from soil and plant surfaces) anomalies during heatwave events—soil evaporation (E) and transpiration (T)—for 268 heatwave events across 32 National Ecological Observatory Network (NEON) flux sites in the contiguous United States from 2019–2021. Using an ensemble of four high-frequency turbulence methods (Flux-variance Similarity, Conditional Eddy Covariance [CEC], CEC with Water-Use Efficiency, and Conditional Eddy Accumulation; see Zahn and Bou-Zeid 2024), half-hourly transpiration-to-evapotranspiration (T/ET) ratios were derived from 20 hertz (Hz, cycles per second) eddy covariance measurements of carbon dioxide (CO₂) and water vapor (H₂O) concentrations. The dataset spans six vegetation types including evergreen and deciduous forests, grasslands, cultivated crops, shrublands, and emergent herbaceous wetlands. Data Package Contents: The dataset includes a single CSV (comma-separated values) file containing daily anomalies (deviations from baseline conditions) for transpiration (Delta_T), evaporation (Delta_E), total evapotranspiration (Delta_ET), and T/ET ratio (Delta_T_ET) during each day of identified heatwave events. The file also includes site codes, dates, heatwave event identifiers, and day-of-heatwave indicators. The CSV file can be opened with spreadsheet software (Microsoft Excel, Google Sheets) or programming environments (Python, R, MATLAB). This resource enables researchers to investigate ecosystem-specific responses to thermal extremes, validate land surface model partitioning of ET fluxes, and examine feedbacks between water cycling and surface energy balance during heatwaves. The dataset is particularly valuable for studies linking vegetation hydraulic strategies to climate resilience, as it captures the divergent responses of shallow-rooted versus deep-rooted ecosystems. Potential applications include improving drought early warning systems, informing irrigation management strategies, and advancing our mechanistic understanding of land-atmosphere interactions under extreme heat conditions.

Day of Heatwave↗

Post survey report for AOP Assignable Asset collection of Crested Butte, CO

This report contains details of the National Ecological Observatory Network Airborne Observation Platform (NEON AOP) assignable asset (AA) flight over Crested Butte, Colorado (CO). The report is being prepared for Lawrence Berkeley National Lab (LBNL), the primary clients for the flights who contracted the NEON AOP for flights during June 2018. The report includes a summary of the acquired flight lines over the planned area, results of calibration surveys, and quality assurance (QA) results of the acquired data. Detailed reports on the acquired data and processing results were also delivered with the data. LBNL contracted the AA NEON AOP flights to observe watersheds of interest at Crested Butte with remotely sensed data including high resolution LiDAR, imaging spectroscopy, and high-resolution camera imagery. The remote sensing imagery acquired by the AOP intendeds to support LBNL on an interdisciplinary project on hydrology, biogeochemistry, and ecosystem functioning in the area surrounding the Rocky Mountain Biological Laboratory (RMBL), one of the most prominent ecological study sites in the United States.

2018 NEON and 2025 CHESS Campaigns↗

CHESS 2025: Location data for field observations and sampling

This dataset represents geolocation data associated with field observations and sampling from the Colorado Headwaters Ecological Spectroscopy Study (CHESS) during June and July of 2025. Location data were collected using Trimble DA2 Global Navigation Satellite System (GNSS) receivers with Trimble Catalyst 2 centimeter (cm) positioning service and the Environmental Systems Research Institute (Esri) Field Maps mobile app. Files in this data package include meadow site polygons, shrub site polygons, tree site polygons and stem point locations, and Leaf Area Index (LAI) plot polygons (.geojson). The geojson files can be opened with open-source GIS software (e.g, QGIS). A csv file is also provided with point coordinates for all locations. CHESS Project Description: The Colorado Headwaters Ecological Spectroscopy Study (CHESS) comprised a multi-week airborne remote sensing and field observation campaign in the Upper Gunnison Basin, Colorado, conducted in June and July of 2025. Airborne remote sensing was conducted by the National Ecological Observatory Network Airborne Observation Platform (NEON AOP), concurrent with a field campaign run by the Rocky Mountain Biological Laboratory (RMBL), the Lawrence Berkeley National Laboratory (LBNL) and SLAC National Accelerator Laboratory Watershed Function Science Focus Area (SFA), and NASA-JPL (Jet Propulsion Laboratory) Earth Surface Mineral Dust Source Investigation (EMIT) program. Between June 10 and July 18, 2025, the NEON AOP flight team collected high-resolution aerial imaging spectroscopy and Light Detection and Ranging (LiDAR) data over three domains: the Upper East River (CRBU), Almont Triangle (ALMO), and the Upper Taylor Basin (UPTA). In coordination with the flights, a field campaign acquired ground-truth observations, including observations of vegetation composition, foliar traits, forest demography, and subsurface properties in 18 core sampling areas within the domains. Additional surface water observations were taken at over 380 point locations. All CHESS campaign datasets can be found within the CHESS ESS-DIVE data portal: https://data.ess-dive.lbl.gov/portals/chess. Funding Acknowledgment: Field and remote-sensing data acquisition was performed under a grant from the National Aeronautics and Space Administration (80NSSC24K1005). This work was also supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS Campaigns↗

Water Observations of Flow/No-Flow for the East-Taylor Watershed, Colorado (June-July 2025 and 2026)

This dataset provides multi-year, ground-truth visual observations of surface water flow/no-flow conditions within the East-Taylor Watershed, Colorado, collected during June and July of 2025 and 2026. In June and July 2025, on-the-ground visual observations of flow/no-flow were collected as part of the Watershed Function Scientific Focus Area (SFA) and Rocky Mountain Biological Laboratory (RMBL) Colorado Headwaters Ecological Spectroscopy Study (CHESS) campaign (further details are provided within the CHESS Project Description). We obtained 377 water observations of flow/no-flow within the East-Taylor Watershed, Colorado. These ground-truth observations were collected to validate classification maps from remote sensing data and model results within the East-Taylor Watershed. In 2025, flow/no-flow measurements were collected using a field-based app for the CHESS Campaign (Zerion iForm). Within the field app, a water observation form was created to collect coordinates and metadata about the observation. Information collected for the water observation points included information about visually-assessed streamflow presence/absence (standard question obtained from Colorado State University’s StreamTracker project), flow estimate, stream or ponded area width, canopy cover, manganese films, iron seeps, and beaver activity. For 2025 water observations, this dataset contains: (1) a data file with the water observations and coordinates (2025_Water_Observations.csv); (2) a Keyhole Markup Language Zipped (KMZ) with the water observation locations and metadata (2025_Water_Observations_Locations.kmz); (3) photos (.jpg and .jpeg) of the water observation points, organized by location, contained within 2025_Water_Observations_FieldPhotographs.zip file; and (4) water observation protocols and figures (2025_Water_Observation_Protocols.pdf). In June and July 2026, on-the-ground visual observations of flow/no-flow were collected as part of the Watershed Function SFA project. We obtained 365 water observations of flow/no-flow within the East-Taylor Watershed, Colorado. The 2026 observations focused on collecting repeat measurements at the 2025 flow/no-flow observation locations conducted as part of the CHESS campaign. These ground-truth observations were collected to understand differences in flow/no-flow in 2026, given the unprecedented 2026 drought in Colorado. In 2026, flow/no-flow measurements were collected using ArcGIS (Geographic Information System) Survey123. Within the field app, a water observation form was created to collect coordinates and metadata about the observation. Information collected for the water observation points included repeat information from the 2025 water observation effort, including visually-assessed streamflow presence/absence (standard question obtained from Colorado State University’s StreamTracker project), flow estimate, stream or ponded area width, canopy cover, manganese films, iron seeps, beaver activity, and a new metadata component of estimated stream depth (for select locations). For 2026 water observations, this dataset contains: (1) a data file with the water observations and coordinates (2026_Water_Observations.csv); (2) a Keyhole Markup Language Zipped (KMZ) with the water observation locations and metadata (2026_Water_Observations_Locations.kmz); (3) photos (.jpg) of the water observation points, organized by location, contained within 2026_Water_Observations_FieldPhotographs.zip file; and (4) water observation protocols and figures (2026_Water_Observation_Protocols.pdf). For 2025 and 2026 water observations, this dataset contains: (1) a location metadata file (locations.csv); (6) a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata; and (7) a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type. CHESS Project Description: The Colorado Headwaters Ecological Spectroscopy Study (CHESS) comprised a multi-week airborne remote sensing and field observation campaign in the Upper Gunnison Basin, Colorado, conducted in June and July of 2025. Airborne remote sensing was conducted by the National Ecological Observatory Network Airborne Observation Platform (NEON AOP), concurrent with a field campaign run by the Rocky Mountain Biological Laboratory (RMBL), the Lawrence Berkeley National Laboratory (LBNL) and SLAC National Accelerator Laboratory Watershed Function Science Focus Area (SFA), and NASA-JPL (Jet Propulsion Laboratory) Earth Surface Mineral Dust Source Investigation (EMIT) program. Between June 10 and July 18, 2025, the NEON AOP flight team collected high-resolution aerial imaging spectroscopy and Light Detection and Ranging (LiDAR) data over three domains: the Upper East River (CRBU), Almont Triangle (ALMO), and the Upper Taylor Basin (UPTA). In coordination with the flights, a field campaign acquired ground-truth observations, including observations of vegetation composition, foliar traits, forest demography, and subsurface properties in 18 core sampling areas within the domains. Additional surface water observations were taken at over 380 point locations. All CHESS campaign datasets can be found within the CHESS ESS-DIVE data portal: https://data.ess-dive.lbl.gov/portals/chess. This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231. 2026-09-02: This dataset was updated to include 2026 water observation measurements. The 2025 observation files were also updated to ensure a consistent file naming convention across water observation years.

2018 NEON and 2025 CHESS Campaigns↗

A hybrid Penman-Monteith and machine learning model for simulating evapotranspiration and its components

Integrating physical processes with machine learning has advanced evapotranspiration (ET) simulation, yet most hybrid models fail to partition total ET into its components: soil evaporation (E) and vegetation transpiration (T). This study introduces Residual Neural Network–Penman–Monteith (RNN-PM), a novel hybrid dual-source ET model designed to overcome this limitation. The model synergizes the physically-based Penman–Monteith framework with three specialized residual neural networks trained to estimate key conductance parameters (canopy conductance, soil surface conductance, and aerodynamic conductance). Furthermore this explicit parameterization allows for the direct partitioning of total ET. Validation at National Ecological Observatory Network (NEON) flux sites using high-frequency partitioned E and T shows that RNN-PM reliably reproduces ET and the transpiration fraction (T/ET). For ET, the model achieves an average Kling–Gupta efficiency (KGE) of 0.89 and a root-mean-square error (RMSE) of 0.55 mm/day; for T/ET, the KGE is 0.87 with an RMSE of 0.06. Furthermore, RNN-PM demonstrates robust generalization, accurately simulating ET and its components well beyond the initial training dataset, even under extreme climatic conditions. This study extended the analysis by comparing the RNN-PM model with seven established dual-source ET models. The results indicate that RNN-PM outperforms both conventional machine learning models and purely physical process-based models in simulating ET components in most cases. Among the purely physical process-based dual-source models, those based on surface temperature decomposition showed improved performance as the leaf area index (LAI) decreased when evaluated against high-frequency ET component datasets. In contrast, the performance of conductance-based dual-source models declined with decreasing LAI. Although purely machine learning-based models can produce relatively accurate simulations of ET components, they often exhibit limited generalization capability, an issue that the RNN-PM model effectively overcomes. Ultimately, the RNN-PM model represents a significant advance in simulating ET components, offering a novel and scalable approach for improving the representation of land–atmosphere interactions in Earth system models.

54 ENVIRONMENTAL SCIENCES↗

Distributed Acoustic Sensing (DAS) on Opportunistic Networks_ A Feasibility Study Utilizing Fiber Optic Infrastructure at NEON Sites for DAS

This study demonstrates the feasibility of using Distributed Acoustic Sensing (DAS) on the opportunistic optical fiber infrastructure presented by NEON (National Ecological Observatory Network) sites. The test took place at the NEON Terrestrial Site designated D10 CPER. Unlit portions of the local fiber network were successfully spliced together and optimized for length and a Silixa iDAS sensor was installed. The resulting array (composed of 12 spliced fiber runs) totaled 875m in length. Over a period of 2 weeks, we gathered a high spatial and temporal resolution data set to characterize discernable signals and understand background noise. The data set was reviewed to extract wave signatures of identifiable origin, both anthropogenic and otherwise. Continent wide distribution of these sites makes them an interesting candidate for DAS and identifying measurable signal sources informs future DAS application on opportunistic fiber networks. These observations validate the utility of both the NEON and similarly small fiber networks (<1km) for geoscience, environmental, and security DAS applications and indicate possibilities for further inquiry in methods for DAS data collection and analysis.

42 ENGINEERING↗

Data and scripts associated with “Sequential Precipitation Input Tagging (SPIT) to Estimate Water Transit Times and Hydrologic Tracer Dynamics within Water-Tagging Enabled Hydrologic Models” (v3)

This data package is associated with the publication “Sequential Precipitation Input Tagging (SPIT) to Estimate Water Transit Times and Hydrologic Tracer Dynamics within Water-Tagging Enabled Hydrologic Models” submitted to Journal of Advances in Modeling Earth Systems (Butler et al. 2025). This study developed the Sequential Precipitation Input Tagging (SPIT) framework to tag input precipitation and estimate water transit times and hydrologic tracers. SPIT tags all precipitation events at regular intervals over an extended period (monthly tags over seven years) in a hydrologic model from 2016-2022. SPIT is applied at six National Ecological Observatory Network (NEON) sites across the continental United States to calculate transit time distributions (TTD) and derive from these mean transit times (MTT), fractions of young water (Fyw), and hydrologic tracer concentrations in stream water (δ18O) within a water-tagging enabled version of the Weather Research and Forecast (WT-WRF-Hydro) model with national water model (NWM) configurations. We go on to validate WT-WRF-Hydro estimates against Butler et al. (2023), who analyzed the same NEON sites using stable water isotope data to estimate water transit times. This new tracking method provides a detailed picture of water movement and helps improve predictions about water availability in the future. This data package was originally published in January 2025. It was updated May 2025 (v2; new and modified files) and October 2025 (v3; new and modified files). File and folder names were not revised to indicate changes. See the change history section in the readme for more details. This data package contains the data and scripts used to develop the SPIT framework WT-WRF-Hydro (Water Tagging Weather Research and Forecasting Hydrologic) model and is associated with the following GitHub repository: https://github.com/zbutler33/SPIT-Framework. This data package contains five parent folders: (1) “Manipulated_outputs”, (2) “Metadata”, (3) “Observed”, (4) “Outputs”, and (5) “Scripts”. Each of these parent folders contains additional subfolders and files. Please see the FLMD (“v*_Butler_2024_WT_WRF_Hydro_flmd.csv”) for a list of all the files contained in this data package and descriptions for each. See the data dictionary (“v*_Butler_2024_WT_WRF_Hydro_dd.csv”) for definitions and units of all of the tabular (files ending in “.csv” and ".tsv") column headers.

54 ENVIRONMENTAL SCIENCES↗

Geophysical survey associated with NEON AOP survey, East River, CO 2018

The package contains data layers developed and used in Falco et al. 2024: “EcoImaging: Advanced Sensing to Investigate Plant and Abiotic Hierarchical Spatial Patterns in Mountainous Watersheds". The package is part of the DOE Watershed Function Science Focus Area (SFA) project and includes geophysical measurements collected at the East River, Colorado, in conjunction with the National Ecological Observatory Network (NEON) Airborne Observation Platform (AOP) survey conducted in June 2018. This dataset provide soil geophysical information and were used to investigate soil-plant relationships. The dataset consists of: - NEON_2018_EMI_survey.zip: the electromagnetic induction (EMI) survey as shape-file; - NEON_plot_TDR.csv: plot‑level data from Time‑Domain Reflectometry (TDR) measurements, providing: * volumetric water content (VWC) in percent (%); * soil temperature in degrees Celsius (°C); - file level metadata (flmd.csv) - data dictionary (dd.csv) file This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS Campaigns↗

Soil physical and chemical measurements for topsoils collected during NEON campaign in East River, CO (06/14/2018-06/28/2018)

The package is part of the DOE Watershed Function Science Focus Area (SFA) project and includes soil physical and chemical measurements from topsoils collected at the East River, Colorado, in conjunction with the National Ecological Observatory Network (NEON) Airborne Observation Platform (AOP) survey conducted in June 2018. The soil measurements include soil bulk density, soil volumetric water content, soil microbial biomass C (Carbon), N (Nitrogen) and C:N (C to N ratio), soil DNA yield, soil total extractable organic C, soil total extractable N, soil extractable nitrate, soil extractable ammonium, soil dissolved inorganic N, soil dissolved organic N, soil pH, soil TOC400 (total organic carbon at 400°C), soil ROC (residual oxidizable carbon), soil TIC (total inorganic carbon), soil TOC (total organic carbon), soil TC (total carbon), soil N, soil OM (organic matter) loss on ignition. Additional associated site metadata can be found in the ESS-DIVE package 10.15485/1618130. The dataset includes (1) 2018_NEON_soil_physical_chemical_measurements.csv: soil physical and chemical measurements indexed by soil sample IGSNs; (2) samples.csv: sample metadata file used to register International Generic Sample Numbers (IGSNs); (3) flmd.csv: file level metadata file; and (4) dd.csv: data dictionary file. This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS (Catchment Hydrology and ↗

NEON AOP Survey of Upper East River CO Watersheds: LAZ Files, LiDAR Surface Elevation, Terrain Elevation, and Canopy Height Rasters

Lawrence Berkeley National Laboratory (LBNL) contracted the National Ecological Observatory Network Airborne Observation Platform (NEON AOP) to observe watersheds of interest surrounding Crested Butte, CO with remotely sensed data, including LiDAR. The flight box design encompassed the watersheds, surveying a total area of 334 km2 across 72 lines. The instrument used was an Optech Gemini, with a pulse density of 2-9 pulses m-2 across the study area (see final report document for detailed information (Goulden and Musinsky 2020). These LiDAR data are the primary data that were provided by NEON, and include both LiDAR source data and derived surface products. The source data are contained in 'crbu_laz.zip' and 'classified_point_cloud.zip. 'classified_point_cloud.zip' represents an additional processing level on 'crbu_laz.zip'. The derived products are 'dsm_mosaic_min_phase_me.tif', 'dtm_mosaic_min_phase_me.tif', and 'tch_mosaic_min_phase_me.tif'. These files are raster mosaics of a digital surface elevation model (DSM), digital terrain elevation model (DTM), and a canopy height model (CHM) at 1 m resolution with a height threshold of > 2 m, respectively, in geotiff (.tif) format. This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231. [UPDATE] On 06/26/2026 the files 'crbu_laz.zip' and 'classified_point_cloud.zip' were replaced with original output files from NEON to address zip file corruption and naming errors. No changes were made to the underlying data. (See Methods Step 2 for details).

2018 NEON and 2025 CHESS Campaigns↗