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CELSS for advanced manned mission

An overview of the major concepts of Controlled Ecological Life Support System (CELSS) includes an identification of environmental factors, such as gravity levels, light levels, and growth volume, that influence the type of CELSS system that can be developed. Various plant growth systems are described together with their possible space applications. Life support functions performed by plants include food production, atmosphere regeneration, and water purification. Selected relationships between biological and physical-chemical life support techniques are considered as a part of these functions. Consumers in a CELSS may be humans, animals, or microorganisms, but nutritional, water, and atmosphere requirements of humans are emphasized in this report, as they are the primary requirement drivers for a CELSS design. The human role in waste generation is discussed as it affects plant nutrient availability. The role of waste management systems in recovering nutrients for plant growth and requirements for CELSS are defined for air, water, and food. Both physical and a biological nutrient recovery/waste disposal systems are examined. The separate subsystems of a CELSS are identified and discussed. Nutrient recovery, plant irradiation, automation, and facilities equipment and applications are reviewed with special attention to direct solar irradiation using fiber optics. These subsystems, along with other environmental control systems, such as thermal, humidity, and ventilation, are essential to plant growth in the space environment.

NASA Discipline Life Support Systems

Separation and Purification of Mineral Salts from Spacecraft Wastewater Processing via Electrostatic Beneficiation

Electrostatic separation is a class of material processing technologies commonly used for the sorting of coarse mixtures by means of electrical forces acting on charged or polarized particles. Most if not all of the existing tribo-electrostatic separators had been initially developed for mineral ores beneficiation. It is a well-known process that has been successfully used to separate coal from minerals. Potash (potassium) enrichment where underground salt mines containing large amounts of sodium is another use of this techno logy. Through modification this technology can be used for spacecraft wastewater brine beneficiation. This will add in closing the gap be~een traveling around Earth's Gravity well and long-term space explorations. Food has been brought on all man missions, which is why plant growth for food crops continues to be of interest to NASA. For long-term mission considerations food productions is one of the top priorities. Nutrient recovery is essential for surviving in or past low earth orbit. In our advance bio-regenerative process instead of nitrogen gas produced; soluble nitrate salts that can be recovered for plant fertilizer would be produced instead. The only part missing is the beneficiation of brine to separate the potassium from the sodium. The use of electrostatic beneficiation in this experiment utilizes the electrical charge differences between aluminum and dried brine by surface contact. The helixes within the aluminum tribocharger allows for more surface contact when being agitated. When two materials are in contact, the material with the highest affinity for electrons becomes negatively charged, while the other becomes positively charged. This contact exchange of charge may cause the particles to agglomerate depending on their residence time within the tribocharger, compromising the efficiency of separation. The aim of this experiment is to further the development in electrostatic beneficiation by optimizing the separation of ersatz and possibly real wastewater brine residues. In doing so, ideally it will yield a high potassium enrichment for use in spacecraft plant systems.

Miles, John D., II

Plasma for Crewed Transit and Planetary Habitation

As human exploration moves beyond low Earth orbit, innovative life support and resource production systems become forerunners for human colonization. Plasma systems allow for complex chemical processes while mitigating consumable needs. Plasma technology and applications can redefine the space environment. With this, scientists at NASA are looking to close known space technology gaps and create a paradigm shift in space-based applications. NASA’s Kennedy Space Center is exploring the use of low-temperature plasma for applications in agriculture, sanitation ,regolith reduction, waste gasification, water activation, and nutrient recovery techniques. Using low temperature plasmas, we report on the success criteria and experimental results for plasma applications for crewed transit and planetary habitation.

Plasma

Plasma for Crewed Transit and Planetary Habitation

As human exploration moves beyond low Earth orbit, innovative life support and resource production systems become forerunners for human colonization. Plasma systems allow for complex chemical processes while mitigating consumable needs. Plasma technology and applications can redefine the space environment. With this, scientists at NASA are looking to close known space technology gaps and create a paradigm shift in space-based applications. NASA’s Kennedy Space Center is exploring the use of low-temperature plasma for applications in agriculture, sanitation, regolith reduction, waste gasification, water activation, and nutrient recovery techniques. Using low temperature plasmas, we report on the success criteria and experimental results for plasma applications for crewed transit and planetary habitation.

Waste management

Plasma for the Space Environment: An Overview of the Research at NASA's Kennedy Space Center

Human life amongst other planets requires vast technological advancements to sustain nutritional, environmental, and logistical needs. Plasma systems allow for advanced chemical processing while mitigating consumable needs or reliance on industrial infrastructure. Scientists at NASA’s Kennedy Space Center are attempting to close technology gaps with research into plasmas and their applications. Some such work includes the use of plasma for waste gasification, water activation, nutrient recovery techniques, lunar regolith reduction for oxygen extraction, and space crop production support. Here we report on the needs, success criteria, and results of plasma efforts for crewed transit and planetary habitation.

Plasma

The effect of drying and size reduction pretreatments on recovery of inorganic crop nutrients from inedible wheat residues

Inorganic nutrients can be easily recovered from ALS crop residue solid wastes by aqueous leaching. However, oven drying and milling pretreatment of these residues has been frequently required to accommodate crop scientists and facility storage limitations. As part of a research study that will compare three different bioreactor technologies for processing these wastes, we realized that different drying and size-reduction pretreatments had been utilized for each technology. This paper compares the effects of residue pretreatment on recovery of nutrients by leaching. Pretreatments included three drying methods [fresh, oven-dried (70 degrees C overnight), and freeze-dried] and two size reduction methods [chopped (2 cm length) and milled (2 mm diameter)]. Determination of mass balances (dry weight and ash content of solids) before and after leaching indicated solubilization was least for fresh residues (23% dry weight loss and 50% for ash loss), and most for freeze-dried residues (41-47% dry weight loss and nearly 100% for ash loss). Mineral recovery of major elements (NO3, PO4, K, Ca, and Mg) in leachates was poorest for fresh residues. P and K recovery in leachates were best for oven-dried residues and Ca, Mg, and N recovery best for freeze-dried residues. The differences in recovery for N, P, and K in leachates were minimal between chopping and milling and slightly better for Ca and Mg from milled residues.

NASA Center KSC

Hydroponic Crop Production using Recycled Nutrients from Inedible Crop Residues

The coupling of plant growth and waste recycling systems is an important step toward the development of bioregenerative life support systems. This research examined the effectiveness of two alternative methods for recycling nutrients from the inedible fraction (residue) of candidate crops in a bioregenerative system as follows: (1) extraction in water, or leaching, and (2) combustion at 550 C, with subsequent reconstitution of the ash in acid. The effectiveness of the different methods was evaluated by (1) comparing the percent recovery of nutrients, and (2) measuring short- and long-term plant growth in hydroponic solutions, based on recycled nutrients.

Garland, Jay L.

Development and Validation of a Process Model and Open-Source Process Simulator for Microalgae-Based Tertiary Phosphorus Recovery

Microalgae-based tertiary wastewater treatment has the potential to meet stringent effluent phosphorus limits, with the added benefit of producing a marketable feedstock. However, the lack of validated mechanistic models and their implementation in process simulators have limited the adoption of this technology. In this study, an updated lumped pathway metabolic model (Phototrophic-Mixotrophic Process Model, PM 2 ), including both photoautotrophic and heterotrophic metabolisms of microalgae, was developed to predict effluent phosphorus concentration and biomass yield in response to dynamic influent and varying environmental conditions. The model was implemented in QSDsan – an open-source, Python-based design and simulation platform – for robust simulation under uncertainty. A global sensitivity analysis was performed to prioritize model parameters for calibration. The model was then calibrated and validated using batch experimental data and 45 days of continuous online monitoring data from a full-scale (568 m 3 ·d -1 ) microalgae-based tertiary wastewater treatment plant (EcoRecover process). In particular, along with dynamic influent composition, temperature and light intensity data with diel variation were provided as model inputs to reflect the microalgal behavior under day-night cycling. Overall, the QSDsan-based microalgae process simulator was able to predict effluent phosphorus within 0.02–0.04 mg-P·L -1 , while also capturing the general trends of state variables according to nutrient availability.

Lumped pathway metabolic model

Probing the Mechanism of Selective Phosphate Adsorption from Wastewater Using Aqueous and Synchrotron X-ray Characterization

Ion exchange shows promise for recovering phosphate from wastewater as value-added products, but requires high phosphate selectivity to compete with conventional treatment. Hybrid anion exchange (HAIX) resins, which contain non-selective basic functional groups and selective iron oxide nanoparticles (FeOnp), can effectively remove phosphate from wastewater. However, knowledge gaps remain regarding the mechanisms of phosphate selectivity and influence of competing ions, hindering needed efforts to model adsorption dynamics and design scalable adsorption processes for varying wastewaters. To address these gaps, we integrated aqueous-phase adsorption analysis with solid-phase, synchrotron-based X-ray characterization; this integration facilitated elucidation of the distribution and speciation of iron, phosphate, and competing anions on HAIX resins. We compared a quaternary ammonium-functionalized HAIX resin (SBA) to a tertiary amine version (WBA) to determine the role of functional groups. X-ray radiography revealed differences in FeOnp speciation (goethite vs. ferrihydrite) and distribution (peripheral vs. homogeneous) between the resins, resulting in varied phosphate affinity and intraparticle diffusion resistance. Using micro-X-ray fluorescence (μ-XRF) and micro-X-ray absorption near-edge structure (μ-XANES) spectroscopy, we identified differences in where and how phosphate binds across resin types and wastewaters. Across wastewater compositions, FeOnp sites in WBA contribute more to phosphate adsorption than in SBA, possibly due to variations in Fe distribution and speciation. Phosphate adsorption densities calculated from quantitative μ-XRF maps matched those from aqueous analysis, demonstrating the effectiveness of this integrated approach. Altogether, results demonstrate the use of synchrotron-based X-ray characterization for investigating adsorption mechanisms and advance HAIX as a phosphate recovery technology from complex wastewaters.

Nutrient recovery

A PRACTICAL ELECTRODIALYSIS MODEL FOR ACCELERATING SYSTEM DEVELOPMENT

Empirical optimization of electrodialysis (ED) is dependent on repetitive experiments with incremental adjustments, which is cost prohibitive at scale. While models can reduce the costs associated with optimization and scale-up, existing ED models are limited in application to specific use cases and tend to be developed for the exploration of specific transport phenomena. The field requires a practical system-level model, generalized for the broad range of ED systems. This work presents a modeling framework that enables rapid evaluation of membrane stack design, flow configuration, scale, and operational inputs. Across applications spanning 1 L to 5400 L; use of conventional and bipolar membranes; operation in continuous, batch and fed-batch modes; and feedstocks including seawater, brine, wastewater, and manure hydrolysate, the model achieves a mean R2 of 0.978 for concentration-time profiles and links design choices to techno-economic trade-offs, enabling cost-aware prioritization of system configurations.

Bipolar Membrane

Utilization of the wastes of vital activity

The recycling of wastes from the biological complex for use in life-support systems is discussed. Topics include laboratory equipment, heat treatment of waste materials, mineralization of waste products, methods for production of ammonium hydroxide and nitric acid, the extraction of sodium chloride from mineralized products, and the recovery of nutrient substances for plants from urine.

Gusarov, B. G.

A Novel Approach to Growing Gardens in Space

A Project [eXposed Root On-Orbit Test System (XROOTS™)] to investigate techniques that can be scaled for crop production in microgravity, a NASA-sponsored Sierra Space team is developing soilless nutrient delivery and recovery technologies that will be demonstrated via plant tests aboard the International Space Station.

space crop production

Characterization of the Water Soluble Component of Inedible Residue from Candidate CELSS Crops

Recycling of inorganic nutrients required for plant growth will be a necessary component of a fully closed, bioregenerative life support system. This research characterized the recovery of plant nutrients from the inedible fraction of three crop types (wheat, potato, and soybean) by soaking, or leaching, in water. A considerable portion of the dry weight of the inedible biomass was readily soluble (29 percent for soybean, 43 percent for wheat, and 52 percent for potato). Greater weight loss from potato was a result of higher tissue concentrations of potassium, nitrate, and phosphate. Approximately 25 percent of the organic content of the biomass was water soluble, while the majority of most inorganic nutrients, except for calcium and iron, were recovered in the leachate. Direct use of the leachates in hydroponic media could provide between 40-90 percent of plant nutrient demands for wheat, and 20-50 percent of demand for soybean and potato. Further evaluation of leaching as a component of resource recovery scheme in a bioregenerative system requires study of (1) utilization of plant leachates in hydroponic plant culture; and (2) conversion of organic material (both soluble and insoluble) into edible, or other useful, products.

Garland, Jay

Using Electrostatic Principles to Separate Out Nutrients from ECLSS Wastewater Brines

This project will study the use electrostatics to perform electrostatic separation and recovery of Environmental Control and Life Support Systems (ECLSS) waste brines. Currently we have no way to perform nutrient recovery on ECLSS waste brines. This process can give us a competing way to separate sodium from potassium to allow tandem production of acids, bases, and plant fertilizers to run upstream operations and hydroponic systems respectively (when combined with electrodialysis). If we can recover valuable chemicals from brine, then this will decrease resupply from earth, which will allow tremendous (multiple kilograms a day up-mass reduction) cost savings. In addition, using electrostatics for regolith enrichment allow much reduced mining costs for metal production on celestial bodies and allow a smaller mining footprint.

Michael D. Hogue

Algae Biofilm Produced from Anaerobic Digester Wastewater Demonstrated as Biofertilizer for Dwarf Wheat

Aims: To correlate yield of dwarf wheat fertilized with algae biofilm produced using anaerobic digester effluent (digestate) from a municipal water resource recovery facility as nutrient source and compare with yield using non-algae biofilm based fertilizers and a negative control. Study Design: Greenhouse testing for measurement of biomass yields and statistical analysis of wheat yield data results. Place and Duration of Study: Department of Biological Engineering Algae Processing and Products Facility greenhouse between March and June, 2024. Methodology: Yield of a dwarf cultivar of red spring wheat was measured as a function of four treatments including three fertilizers and a negative control: (1) algae biofilm cultivated on anaerobic digester effluent, (2) positive control of Osmocote commercial slow release fertilizer, (3) anaerobic digester biosolids, and (4) negative control with no fertilizer addition. Results: Average yield values (gm) for the four treatments were: (1) 1.25, (2) 0.99, (3) 0.72, and (4) 0.52, respectively. Algae biofilm fertilizer performed significantly better than Osmocote with P = .002, anaerobic digester biosolids with P = .05, and no fertilizer addition with P = .05. Struvite, a slow release fertilizer, was observed to be associated with the algae biofilm as a precipitate of the high concentrations of nutrients within the anaerobic digester water. Struvite (MgNH4PO46H2O) releases plant available nitrogen and phosphorus over time and is biologically precipitated due to the increase in solution pH as a result of photosynthesis by the algae biofilm. Conclusion: Algae biofilm cultivated on anaerobic digester wastewater can serve as a biofertilizer for recycling nutrients within the biosphere for sustainable nutrient management.

09 BIOMASS FUELS

Detection of Vegetation Trends in Highly Variable Environments After Grazing Exclusion in Mongolia

Aims: Environmental variability (e.g. in precipitation) has a large effect on vegetation dynamics, and this often makes it difficult to assess the recovery of vegetation after a disturbance. In this study, we assessed vegetation recovery trends in response to grazing exclusion while considering for the annual environmental variability. Location: Two regions with highly variable precipitation: a steppe near Mandalgobi, in Mongolia's Central Gobi province, and a desert steppe near Bulgan, in Mongolia's South Gobi province. Methods: Changes in vegetation were observed along grazing gradients at the above two sites, and vegetation thresholds were identified. We established reference plots in pre-threshold areas along the grazing gradients. We removed the impact of livestock grazing at various locations along the grazing gradients by establishing exclosures, and investigated vegetation from 2005 to 2013. We developed a smoothed hierarchical model within a Bayesian framework, and examined the effect of grazing exclusion on vegetation, focusing especially on the extent of grass cover recovery. In addition, we compared soil nutrient conditions in the reference plots and inside and outside each exclosure along the grazing gradients. Results: Temporal trends in the cover of perennial grass in each plot inside and outside of the exclosures largely coincided, irrespective of grazing intensity, and exclosure had no effect (Bulgan) or a negative effect (Mandalgobi) on vegetation recovery. Soil nutrient content was not significantly affected by exclosure at a given distance from the grazing source, but decreased significantly with decreasing grazing intensity. Thus, recovery of the land from a post-threshold state may not be apparent even after 9 yr of grazing exclusion in environments with highly variable precipitation. Conclusion: The effect of exclosure duration on perennial grass cover was limited, even after controlling for environmental variability. Once a vegetation threshold has been crossed, merely removing livestock from the landscape may not be sufficient for that area to recover.

Bayesian statistics;Drought;Environmental variabil

Recovery of resources for advanced life support space applications: effect of retention time on biodegradation of two crop residues in a fed-batch, continuous stirred tank reactor

Bioreactor retention time is a key process variable that will influence costs that are relevant to long distance space travel or long duration space habitation. However. little is known about the effects of this parameter on the microbiological treatment options that are being proposed for Advanced Life Support (ALS) systems. Two bioreactor studies were designed to examine this variable. In the first one, six retention times ranging from 1.3 to 21.3 days--were run in duplicate, 81 working-volume continuous stirred tank reactors (CSTR) that were fed ALS wheat residues. Ash-free dry weight loss, carbon mineralization, soluble TOC reduction, changes in fiber content (cellulose, hemicellulose, and lignin), bacterial numbers, and mineral recoveries were monitored. At short retention times--1.33 days--biodegradation was poor (total: 16-20%, cellulose - 12%, hemicellulose - 28%) but soluble TOC was decreased by 75-80% and recovery of major crop inorganic nutrients was adequate, except for phosphorus. A high proportion of the total bacteria (ca. 83%) was actively respiring. At the longest retention time tested, 21.3 days, biodegradation was good (total: 55-60%, cellulose ca. 70%, hemicellulose - ca. 55%) and soluble TOC was decreased by 80%. Recovery of major nutrients, except phosphorus, remained adequate. A very low proportion of total bacteria was actively respiring (ca. 16%). The second bioreactor study used potato residue to determine if even shorter retention times could be used (range 0.25-2.0 days). Although overall biodegradation deteriorated, the degradation of soluble TOC continued to be ca. 75%. We conclude that if the goal of ALS bioprocessing is maximal degradation of crop residues, including cellulose, then retention times of 10 days or longer will be needed. If the goal is to provide inorganic nutrients with the smallest volume/weight bioreactor possible, then a retention time of 1 day (or less) is sufficient.

Non-NASA Center

Enhancing Resource Recovery through Electro-Assisted Regeneration of an Ammonia-Selective Cation Exchange Resin

Ammonia-selective adsorbents can manage reactive nitrogen in the environment and promote a circular nutrient economy. Weak acid cation exchangers loaded with zinc exhibit high ammonia selectivity but face two implementation barriers: the stability of the zinc–carboxylate bond in complex wastewaters and energy- and logistics-intensive adsorbent regeneration with acidic solutions. In this paper, we examined the stability of zinc–carboxylate bonds in varying solutions (pure ammonium solution, synthetic urine, and real urine) and during electro-assisted regeneration. For electrochemical regeneration, both electrolyte concentration and current density influenced the trade-off between ammonia regeneration and zinc elution. Using 10 mM K 2 SO 4 anolyte at a 0.08 mA/cm 2 current density, we achieved 4% zinc elution and 61% ammonia regeneration. In contrast, using 100 mM K 2 SO 4 at 4.96 mA/cm 2 improved the regeneration efficiency to 97% but eluted 60% of zinc. We found that electrolyte concentration was the key factor influencing the regeneration efficiency of the NH 3 -selective adsorbents. Due to prevalent zinc elution, we designed an in situ procedure for reforming the zinc–carboxylate bond and achieved similar adsorption densities between pre- and post-regenerated resins, thus enabling multiple cycle resin use. Ultimately, this study advances understanding of ammonia-selective resins that can facilitate high-purity, selective, and durable recovery of nutrients from waste streams.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH