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At least 37 records · Page 2

Holo-Omics disentangle drought response and biotic interactions among plant, endophyte and pathogen

Holo-omics provide a novel opportunity to study the interactions among fungi from different functional guilds in host plants in field conditions. We address the entangled responses of plant pathogenic and endophytic fungi associated with sorghum when droughted through the assembly of the most abundant fungal, endophyte genome from rhizospheric metagenomic sequences followed by a comparison of its metatranscriptome with the host plant metabolome and transcriptome. The rise in relative abundance of endophytic Acremonium persicinum (operational taxonomic unit 5 (OTU5)) in drought co-occurs with a rise in fungal membrane dynamics and plant metabolites, led by ethanolamine, a key phospholipid membrane component. The negative association between endophytic A. persicinum (OTU5) and plant pathogenic fungi co-occurs with a rise in expression of the endophyte's biosynthetic gene clusters coding for secondary compounds. Endophytic A. persicinum (OTU5) and plant pathogenic fungi are negatively associated under preflowering drought but not under postflowering drought, likely a consequence of variation in fungal fitness responses to changes in the availability of water and niche space caused by plant maturation over the growing season. Our findings suggest that the dynamic biotic interactions among host, beneficial and harmful microbiota in a changing environment can be disentangled by a blending of field observation, laboratory validation, holo-omics and ecological modelling.

Chen, Peilin↗

Unveiling the transferability of PLSR models for leaf trait estimation: lessons from a comprehensive analysis with a novel global dataset

Leaf traits are essential for understanding many physiological and ecological processes. Partial least squares regression (PLSR) models with leaf spectroscopy are widely applied for trait estimation, but their transferability across space, time, and plant functional types (PFTs) remains unclear. We compiled a novel dataset of paired leaf traits and spectra, with 47 393 records for >700 species and eight PFTs at 101 globally distributed locations across multiple seasons. Using this dataset, we conducted an unprecedented comprehensive analysis to assess the transferability of PLSR models in estimating leaf traits. While PLSR models demonstrate commendable performance in predicting chlorophyll content, carotenoid, leaf water, and leaf mass per area prediction within their training data space, their efficacy diminishes when extrapolating to new contexts. Specifically, extrapolating to locations, seasons, and PFTs beyond the training data leads to reduced R 2 (0.12–0.49, 0.15–0.42, and 0.25–0.56) and increased NRMSE (3.58–18.24%, 6.27–11.55%, and 7.0–33.12%) compared with nonspatial random cross-validation. The results underscore the importance of incorporating greater spectral diversity in model training to boost its transferability. These findings highlight potential errors in estimating leaf traits across large spatial domains, diverse PFTs, and time due to biased validation schemes, and provide guidance for future field sampling strategies and remote sensing applications.

59 BASIC BIOLOGICAL SCIENCES↗

Barriers and variable spacing enhance convective cooling and increase power output in solar PV plants

When the temperature of solar photovoltaic (PV) modules rises, efficiency drops and module degradation accelerates. Thus, it is beneficial to reduce module operating temperatures. Previous studies of solar power plants have illustrated that incoming flow characteristics, turbulent mixing, and array geometry can strongly impact convective cooling, as measured by the convective heat transfer coefficient h. In the fields of heat transfer and plant canopy flow, previous work has shown that system-scale arrangement modifications—e.g., variable spacing, barriers, or windbreaks—can passively alter the flow, enhance turbulent mixing, and influence convection. However, researchers have not yet explored how variable spacing or barriers might enhance convective cooling in solar power plants. Here, high-resolution large-eddy simulations model the air flow and heat transfer through solar power plant arrangements modified with missing modules and barrier walls. We then perform a control volume analysis to evaluate the net heat flux and compute h, which quantifies the influence of these spatial modifications on convective cooling and, thus, module temperature and power output. Installing barrier walls yields the greatest improvements, increasing h by 3.4%, reducing module temperature by an estimated 2.5 °C, and boosting power output by an estimated 1.4% on average. These findings indicate that incorporating variable spacing or barrier-type elements into PV plant designs can reduce module temperature and, thus, improve PV performance and service life.

14 SOLAR ENERGY↗

Design Tool for Estimating Adsorbent Hydrogen Storage System Characteristics for Light-Duty Fuel Cell Vehicles

This work combines materials development with hydrogen storage technology advancements to address onboard hydrogen storage challenges in light-duty vehicle applications. These systems are comprised of the vehicle requirements design space, balance of plant requirements, storage system components, and materials engineering culminating in the development of an Adsorbent System Design Tool that serves as a preprocessor to the storage system and vehicle-level models created within the Hydrogen Storage Engineering Center of Excellence. Computational and experimental efforts were integrated to evaluate, design, analyze, and scale potential hydrogen storage systems and their supporting components against the Department of Energy 2020 and Ultimate Technical Targets for Hydrogen Storage Systems for Light Duty Vehicles. Ultimately, the Adsorbent System Design Tool was created to assist material developers in assessing initial design parameters that would be required to estimate the performance of the hydrogen storage system once integrated with the full fuel cell system.

08 HYDROGEN↗

Performance and transport in the ARC tokamak

The ARC TM tokamak, a high-field (𝐵 𝑇 = 11.4 T) fusion power plant, under development by Commonwealth Fusion Systems, is studied using a suite of integrated modelling tools to predict its fusion power generation (𝑃𝑓⁡𝑢⁢𝑠), transport and confinement properties. Analysis is based off an ARC operational point scoped first with zero-dimensional (0-D) plasma operational contour (POPCON) modelling to produce 1.13 GW of fusion power. A suite of integrated modelling tools (TRANSP, ASTRA and TORAX) were applied to predict the performance and kinetic profiles of the ARC design point, yielding a range of predicted performance spanning from ∼900 to 1300 MW in rough quantitative agreement with POPCON predictions. The sensitivity of these results to uncertain modelling inputs was probed using scans of pedestal boundary conditions around EPED-predicted values (total pressure and temperature ratios), tungsten concentration and seperatrix density around their nominal assumptions. Pedestal pressure and pedestal top (𝑇 𝑖 /𝑇 𝑒 ) play a large role in 1.5-dimensional performance predictions, able to modify the predicted 𝑃 𝑓⁡𝑢⁢𝑠 by a factor of 2 within reasonable assumptions. High-fidelity core nonlinear gyrokinetic profile predictions, performed using CGYRO (Candy et al. 2016 J. Comput. Phys., vol. 324, pp. 73–93) coupled with the PORTALS (Rodriguez-Fernandez et al. 2024 Nucl. Fusion, vol. 64, 076034; Phys. Plasmas, vol. 31, 2024, 062501) framework, yield substantially lower performance (𝑃 𝑓⁡𝑢⁢𝑠 =677 MW) compared with 0-D and medium-fidelity modelling for nominal assumptions, showing that there is non-negligible uncertainty between models and that future work on SPARC may help resolve discrepancies. Lower overall performance results from significantly reduced volume-averaged densities and temperatures, along with reduced levels of density and temperature peaking. Turbulence and transport are largely dominated by ion temperature gradient across the profile, confirmed by both linear stability and the response of the nonlinear fluxes to changes in gradients, with some impact of kinetic ballooning modes in the deep core. This work represents one of the most complete scoping of potential fusion power plant conditions performed to date. The extensive integrated modelling provides confidence in ARC performance approaching 1 GW, while nonlinear gyrokinetic modelling results in open questions into the physics of density and temperature peaking in fusion-power-plant-relevant operational space. A discussion of results and the role that the SPARC tokamak (Creely et al. 2020 J. Plasma Phys., vol. 86, 865860502) will play in informing ARC design, performance and operation is presented.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Monte Carlo Simulation with CAD Interface for Calculation of 3D Maps of Residual Dose (CRADA)

Objective: To develop an easy-to-use software application to predict and mitigate radiation effects in research environment, space instruments, nuclear plants and medical facilities and help nonproliferation and national security efforts. Tech-X will develop standalone software libraries and command-line tools for ( 1) translating CAD into tessellated surfaces and tetrahedral meshes in GDML (for Geant4 and MARS 15), ROOT (for MARS 15) and HDF5 (for compact representation and for the visualization) formats, (2) healing CAD geometries to make them suitable for Monte Carlo simulations; (3) creating uniform and variable Cartesian and cylindrical meshes for detailed scoring; and ( 4) efficient Monte Carlo navigation in CAD geometries. JLAB will finish automation of simulations of residual dose in CAD geometries and integrate Tech-X software into Geant4 and MARS15. Finally, Tech-X will develop a Graphical User Interface to set up and heal CAD geometries, create input files, run and visualize simulations for residual dose. This application will run on local desktops, local and remote clusters and supercomputers and will be made available through public clouds, such as Amazon Web Services.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Global plant trait relationships extend to the climatic extremes of the tundra biome

The majority of variation in six traits critical to the growth, survival and reproduction of plant species is thought to be organised along just two dimensions, corresponding to strategies of plant size and resource acquisition. However, it is unknown whether global plant trait relationships extend to climatic extremes, and if these interspecific relationships are confounded by trait variation within species. We test whether trait relationships extend to the cold extremes of life on Earth using the largest database of tundra plant traits yet compiled. We show that tundra plants demonstrate remarkably similar resource economic traits, but not size traits, compared to global distributions, and exhibit the same two dimensions of trait variation. Three quarters of trait variation occurs among species, mirroring global estimates of interspecific trait variation. Plant trait relationships are thus generalizable to the edge of global trait-space, informing prediction of plant community change in a warming world.

59 BASIC BIOLOGICAL SCIENCES↗

Finding Promising Candidates for Wet Growing Conditions: The Effect of Two Row Spacings on Biomass Production of Four Bioenergy Prairie Cordgrass Populations in a Wet Marginal Land

Demand for energy and the environmental consequences associated with fossil fuel emissions has created a need for alternative energy sources. To avoid conflict over the conversion of existing agricultural land from food production to fuel production, bioenergy crops could instead be cultivated on marginal land. Here, this study compared the performances, biomass yield, tiller density, and lignocellulosic bioenergy feedstock composition of four prairie cordgrass populations with two-row spacings cultivated on a waterlog-prone marginal land, as well as three other bioenergy candidate crops used as controls: big bluestem, M. × giganteus , and switchgrass. Across all populations and spacings, annual biomass yield of prairie cordgrass was 13 Mg ha −1 . Row spacing had significant impacts on prairie cordgrass productivity, with higher biomass yields observed in the 45 cm spacing than in the 90 cm spacing. Feedstock composition (cellulose, hemicellulose, ADL, and ash concentration) was not influenced by row spacing and did not deviate from expected values for growth on agricultural lands. However, biomass yields of the control species M . × giganteus and switchgrass planted in 45 cm spacing were higher than the prairie cordgrass. Our results provide evidence that prairie cordgrass could be a good energy crop with comparable biomass yield production to the energy crops M . × giganteus and switchgrass on waterlogged marginal land.

Bioenergy crops↗

Genetic correlations between switchgrass performance in sward conditions and surrogate measures

Switchgrass (Panicum virgatum L.) breeders use spaced-seedling plots for evaluations of biomass yield, but prior studies are ambiguous about the relationship between performance in spaced plots and commercial seeded sward performance. This study used a split-plot incomplete block design to evaluate 52 half-sib switchgrass families across two years to determine the correlations among four different plot types (including seeded swards) and four individual plant traits. Plots (1.62 m 2 ) that contained 3, 6, and 18 plants had mean yields of 5.92, 8.04, and 11.06 Mg ha –1 , respectively, while seeded-sward plots had a mean of 5.37 Mg ha –1 . In a mixed-model analysis including family-, year-, and plot-type interactions with a spatial-spline correction, the largest proportion of family variance was accounted for by the main family effect (48%), while the plot type-by-family effect was only 17% of family variance. These results indicate that spaced-plant plots are reasonable surrogates for biomass performance in sward conditions. Yield reliability was 0.50 across all plot types with a mean of 0.46 for individual plot types. Surrogates for sward yield varied due to genetic background of the families. Yield of families derived from the Liberty cultivar (n = 32) had genetic correlations with winter survivorship scores (0.66). Diverse lowland ecotype families (n = 20) had genetic correlations with plant height and heading date. This study highlighted a major difference in performance among the plot types, specifically that seeded swards have depressed yield due to high plant density. This should encourage further research for the mechanism behind this difference in performance.

59 BASIC BIOLOGICAL SCIENCES↗

Development of an Experimental Approach to Achieve Spatially Resolved Plant Root-Associated Metaproteomics Using an Agar-Plate System

Plant–microbe interactions in the rhizosphere play a vital role in plant health and productivity. The composition and function of root-associated microbiomes is strongly influenced by their surrounding environment, which is often customized by their host. How microbiomes change with respect to space and time across plant roots remains poorly understood, and methodologies that facilitate spatiotemporal metaproteomic studies of root-associated microbiomes are yet to be realized. Here, we developed a method that provides spatially resolved metaproteome measurements along plant roots embedded in agar-plate culture systems, which have long been used to study plants. Spatially defined agar “plugs” of interest were excised and subsequently processed using a novel peptide extraction method prior to metaproteomics, which was used to infer both microbial community composition and function. As a proof-of-principle, a previously studied 10-member community constructed from a Populus root system was grown in an agar plate with a 3-week-old Populus trichocarpa plant. Metaproteomics was performed across two time points (24 and 48 h) for three distinct locations (root base, root tip, and a region distant from the root). The spatial resolution of these measurements provides evidence that microbiome composition and expression changes across the plant root interface. Interrogation of the individual microbial proteomes revealed functional profiles related to their behavioral associations with the plant root, in which chemotaxis and augmented metabolism likely supported predominance of the most abundant member. This study demonstrated a novel peptide extraction method for studying plant agar-plate culture systems, which was previously unsuitable for (meta)proteomic measurements.

59 BASIC BIOLOGICAL SCIENCES↗

Whole-Ecosystem Warming Increases Plant-Available Nitrogen and Phosphorus in an Ombrotrophic Bog

Warming is expected to increase the net release of carbon from peatland soils, contributing to future warming. This positive feedback may be moderated by the response of peatland vegetation to rising atmospheric [CO 2 ] or to increased soil nutrient availability. We asked whether a gradient of whole-ecosystem warming (from + 0 °C to + 9 °C) would increase plant-available nitrogen and phosphorus in an ombrotrophic bog in northern Minnesota, USA, and whether elevated [CO 2 ] would modify the nutrient response. We tracked changes in plant-available nutrients across space and through time and in comparison with other nutrient pools, and assessed whether nutrient warming responses were captured by a point version of the land-surface model, ELM-SPRUCE. We found that warming exponentially increased plant-available ammonium and phosphate, but that nutrient dynamics were unaffected by elevated [CO 2 ]. The warming response increased by an order of magnitude between the first and fourth year of the experimental manipulation, perhaps because of dramatic mortality of Sphagnum mosses in the surface peat of the warmest treatments. However, neither the magnitude nor the temporal dynamics of the responses were captured by ELM-SPRUCE. Here, relative increases in plant-available ammonium and phosphate with warming were similar, but the response varied across raised hummocks and depressed hollows and with peat depth. Plant-available nutrient dynamics were only loosely correlated with inorganic and organic porewater nutrients, likely representing different processes. Future predictions of peatland nutrient availability under climate change scenarios must account for dynamic changes in nutrient acquisition by plants and microbes, as well as microtopography and peat depth.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux FLUXNET-1F US-Dk3 Duke Forest - loblolly pine

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Dk3 Duke Forest - loblolly pine. This is the FLUXNET version of the carbon flux data for the site US-Dk3 Duke Forest - loblolly pine produced by applying the standard ONEFlux (1F) software. Site Description - The site was established in 1983 following a clear cut and a burn. Pinus taeda L. (loblolly pine) seedlings were planted at 2.4m by 2.4m spacing and ecosystem development has not been managed after planting. Canopy height increased from 16m in 2001 to 18m in 2004. The canopy is comprised primarily of P. taeda with some emergent Liquidambar styraciflua L. and a diverse and growing understory with 26 different woody species of diameter breast height 42.5 cm. The flux tower lies upwind of the CO2-enriched components of the free atmosphere carbon enrichment (FACE) facility located in the same pine forest. EC instrumentation is at 20.2m on a 22m tower.

Oishi, Chris [USDA Forest Service]↗

Filling the Gaps: A Bayesian Mixture Model for Imputing Missing Soil Water Content Data

ABSTRACT Soil water content (SWC) data are central to evaluating how soil moisture varies over time and space and influences critical plant and ecosystem functions, especially in water‐limited drylands. However, sensors that record SWC at high frequencies often malfunction, leading to incomplete timeseries and limiting our understanding of dryland ecosystem dynamics. We developed an analytical approach to impute missing SWC data, which we tested at six eddy flux tower sites along an elevation gradient in the southwestern United States. We impute missing data as a mixture of linearly interpolated SWC between the observed endpoints of a missing data gap and SWC simulated by an ecosystem water balance model (SOILWAT2). Within a Bayesian framework, we allowed the relative utility (mixture weight) of each component (linearly interpolated vs. SOILWAT2) to vary by depth, site and gap characteristics. We explored “fixed” weights versus “dynamic” weights that vary as a function of cumulative precipitation, average temperature, and time since the start of the gap. Both models estimated missing SWC data well ( R 2 = 0.70–0.88 vs. 0.75–0.91 for fixed vs. dynamic weights, respectively), but the utility of linearly interpolated versus SOILWAT2 values depended on site and depth. SOILWAT2 was more useful for more arid sites, shallower depths, longer and warmer gaps and gaps that received greater precipitation. Overall, the mixture model reliably gap‐fills SWC, while lending insight into processes governing SWC dynamics. This approach to impute missing data could be adapted to accommodate more than two mixture components and other types of environmental timeseries.

Ogle, Kiona [School of Informatics, Computing, and↗

Reproductive Responses to Increased Shoot Density and Global Change Drivers in a Widespread Clonal Wetland Species, Schoenoplectus americanus

The expansion of many wetland species is a function of both clonal propagation and sexual reproduction. The production of ramets through clonal propagation enables plants to move and occupy space near parent ramets, while seeds produced by sexual reproduction enable species to disperse and colonize open or disturbed sites both near and far from parents. The balance between clonal propagation and sexual reproduction is known to vary with plant density but few studies have focused on reproductive allocation with density changes in response to global climate change. Schoenoplectus americanus is a widespread clonal wetland species in North America and a dominant species in Chesapeake Bay brackish tidal wetlands. Long-term experiments on responses of S. americanus to global change provided the opportunity to compare the two modes of propagation under different treatments. Seed production increased with increasing shoot density, supporting the hypothesis that factors causing increased clonal reproduction (e.g., higher shoot density) stimulate sexual reproduction and dispersal of genets. The increase in allocation to sexual reproduction was mainly the result of an increase in the number of ramets that flowered and not an increase in the number of seeds per reproductive shoot, or the ratio between the number of flowers produced per inflorescence and the number of flowers that developed into seeds. Seed production increased in response to increasing temperatures and decreased or did not change in response to increased CO2 or nitrogen. Results from this comparative study demonstrate that plant responses to global change treatments affect resource allocation and can alter the ability of species to produce seeds.

54 ENVIRONMENTAL SCIENCES↗

Root traits reveal safety and efficiency differences in grasses and shrubs exposed to different fire regimes

Plant roots are important components of terrestrial ecosystems, but little is known about how root traits differ between plant species or vary across space in natural ecosystems. In this study, we investigated how root traits vary across grasses and woody shrubs in a tallgrass prairie located in Manhattan, KS, USA. Specifically, were interested in understanding how anatomical traits vary across these different plant groups, across different depths in the soil, and also across multiple areas of tallgrass prairie that are burned at different frequencies. We compared traits that allow roots to use soil water efficiently and those that help roots persist through extended periods of drought. We found that woody roots exhibited traits that allow them to use water efficiently in shallow soils but persist through drought in deeper soil. Here, these traits may allow shrubs to transport water from shallow soils to deeper soil after large rain events and resist extended periods without rain when soils become dry, which may allow woody plants to successfully compete for water in less frequently burned, more diverse plant communities. Grasses had traits that allow them to use water efficiently but also tolerate drought in shallow soils, regardless of fire treatment. This unique trait combination likely gives grasses a competitive advantage over other tallgrass prairie plants across different fire regimes. Together, these results show that anatomical root traits can be used to enhance mechanistic understanding of plant functioning in dynamic ecosystems.

54 ENVIRONMENTAL SCIENCES↗

An Investigation of Heuristic Control Strategies for Multi-Electrolyzer Wind-Hydrogen Systems Considering Degradation

With the growing demand for renewable-energy-powered hydrogen generation and the corresponding increase in plant capacity, individually controlling many electrolyzer stacks will be critical for increasing the plant's lifetime and efficiency. This paper introduces a rule-based controller framework targeting electrolyzer degradation to explore the opportunity space in multi-stack hydrogen plant control. A novel control-oriented degradation model is also presented in this work, which quantifies the impact of steady power input, fluctuating power input, and the number of startup/shutdown cycles on electrolyzer degradation. Using a wind power input signal, 13 controller configurations are tested on 5 MW hydrogen plants consisting of 2, 5, 10, and 25 stacks. These configurations are evaluated on their capability to mitigate electrolyzer degradation and efficiently produce hydrogen from the time-varying input power. The results show that multi-stack control for degradation can extend the plant's lifetime by more than a factor of 3 with minimal impact on instantaneous hydrogen production.

control↗

Assessment of the burning-plasma operational space in ITER by using a control-oriented core-SOL-divertor model

In future tokamaks, the control of burning plasmas will require careful regulation of the plasma density and temperature. Along with the design of effective burn-control systems, understanding how the fusion power varies in the density-temperature space is vital for the operation of fusion power plants. Here in this work, the steady-state operational space of ITER is studied using a control-oriented core-plasma model coupled to a two-point model of the scrape-off-layer (SOL) and divertor regions. The two models are coupled through the exchange of input-output parameters. The deuterium and tritium recycling from the wall are output parameters of the SOL-divertor model that are used as input parameters in the core-plasma density balance. Furthermore, the separatrix temperature, which is an output parameter of the SOL-divertor model, is incorporated into the radial core-plasma temperature profiles. Therefore, the temperature-dependent power balance of the plasma core is intimately linked to the SOL-divertor model. Both the power entering the SOL from the core, as determined by the core-plasma power balance, and the separatrix density, as dictated by the core-plasma density balance, are input parameters to the SOL-divertor model. They are control knobs in the SOL-divertor model that can be regulated using the core-plasma actuators: auxiliary power and pellet injection. There are various operational limitations, such as the saturation of the aforementioned actuators, that will prevent ITER from accessing certain high-fusion plasma regimes. The achievable tritium concentration in the fueling lines and the maximum sustainable heat load on the divertor will impose further restrictions. By accounting for these limitations, the ITER operational space is computed based on the coupled core-SOL-divertor model and visualized using Plasma Operation Contour (POPCON) plots that map performance metrics, such as the fusion to auxiliary power ratio, over the density-temperature space. Comparisons are drawn between plasmas with different recycling, confinement, and SOL-divertor conditions.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

What lies beneath: Vertical temperature heterogeneity in a Mediterranean woodland savanna

As the availability of satellite and airborne thermal infrared remote sensing (TIR-RS) data increases and their spatial, temporal, and spectral resolutions improve, researchers are finding diverse applications for TIR-RS measurements. TIR-RS is now commonly applied in regional- and continental-scale analyses, such as those focused on fire and surface energy balance. However, its application lags in plant physiology and ecology, for which a finer-scale understanding of plant canopy temperatures would be useful to elucidate plant water dynamics, for example. In particular, while methods to disaggregate TIR-RS pixels in horizontal space have advanced, possible vertical stratification of plant canopy temperature and its implications for understanding the correspondence between TIR-RS and finer-scale, field-based thermal measurements (e.g. made with a thermal camera) remain unexplored. Here, we use data from a thermal camera deployed concurrently with the recent ECOSTRESS mission to quantify vertical temperature gradients within tree canopies and temperatures of over- vs. under-story plants in a Mediterranean woodland savanna. We then leverage diverse ancillary data to maximize the geometric comparability of ECOSTRESS and thermal camera measurements, in order to assess the extent to which the two forms of thermal measurements correspond. Specifically, we ask: (1) What are the patterns of intra-canopy and over- vs. under-story vertical temperature in a Mediterranean woodland savanna?, and (2) How can vertically-resolved, but spatially-limited field-based temperature measurements be reconciled with spatially-extensive, but surface-only, temperature measurements of a space-borne remote sensor? Further, we found consistent patterns of vertical thermal heterogeneity both within tree canopies and between ecosystem over- and under-stories. The daytime difference between the top and bottom thirds of blue oak canopies was, on average, 0.48 ° C – and sometimes several times larger. Notably, canopy tops are cooler, likely associated with the under-story grass reaching daytime temperatures often exceeding over-story temperatures by 10° C. Given the consistency of the intra-canopy temperature gradients, we expected the ECOSTRESS sensor would be in better agreement with camera measurements of canopy tops than bulk canopies or canopy bottoms. However, within-canopy gradients were overwhelmed by other sources of disagreement between the measurements, in part associated with upscaling camera measurements across space. Overall, thermal camera and ECOSTRESS measurements were largely in agreement at night (pixel RMSE = 1.1°C), but they were more divergent during times of low (but >0 W/m2) and high incoming solar radiation (daytime pixel RMSE = 3.5°C).

54 ENVIRONMENTAL SCIENCES↗