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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 37 records · Page 2
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Symbiotic bacteriophages exhibit multiple adaptive strategies in activated sludge flocs and contribute to floc stability

Despite the importance of phages for the dynamics and functions of microbial communities, it remains largely unexplored how symbiotic phages adapt in activated sludge systems and influence microbial aggregate stability, which is critical for clarification and reliable performance. Here, based on 12,127 phage contigs recovered from 12 activated sludge microbiomes, the symbiotic phages exhibited broad host ranges and infected dominant prokaryotes in activated sludge. Moreover, these phage communities exhibited high lysogenicity (49% to 66% lysogenic phages) and harbored diverse auxiliary metabolic genes (AMGs) that could enhance microbial aggregate stability, such as genes coding for lipopolysaccharide, peptidoglycan, and extracellular polysaccharide biosynthesis. Additionally, prokaryotic antiviral systems were widely distributed in dominant prokaryotes, particularly the Restriction-Modification (RM) and CRISPR-Cas systems, which could mitigate potential harmful phage infections. Overall, this study reveals the contemporary beneficial relationship between phages and prokaryotic hosts in activated sludge microbiomes and the potential benefits of symbiotic phages on floc stability.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2021

Influence of PRE-emergence herbicides on soybean development, root nodulation and symbiotic nitrogen fixation

We report widespread occurrence of herbicide-resistant weeds has further limited effective POST-emergence herbicide options in soybean (Glycine max (L.) Merr.) leading to an increased adoption of PRE-emergence herbicides. The objective of this study was to investigate the influence of 11 commonly used PRE herbicides on soybean development, root nodulation, and symbiotic N fixation. Soybean plants were grown under greenhouse conditions in pots (10 L; 4 plants per pot) filled with silt loam soil and treated one day after planting with a labeled field rate of imazethapyr, chlorimuron-ethyl, cloransulam-methyl, metribuzin, sulfentrazone, flumioxazin, saflufenacil, acetochlor, S-metolachlor, dimethenamid-P, pyroxasulfone and no herbicide (nontreated control). Sulfentrazone reduced soybean canopy at the VC growth stage but no canopy reduction was observed at the V2 growth stage from any of the herbicide treatments. At the R2 growth stage, herbicides had no effect on soybean development (root and shoot biomass), root nodulation (# nodule per plant, nodule diameter, and nodule biomass) and symbiotic N fixation (acetylene reduction assay and 15 N natural abundance). According to our findings, although PRE herbicides may slightly affect early-season soybean development, the impacts on plant growth, root nodulation, and symbiotic N fixation were negligible. Thus, when sprayed according to the label, the benefits of PRE herbicides for weed control likely outweigh any potential concern regarding soybean development, root nodulation, and N fixation.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Transportome remodeling of a symbiotic microalga inside a planktonic host

Abstract Metabolic exchange is one of the foundations of symbiotic associations between organisms and is a driving force in evolution. In the ocean, photosymbiosis between heterotrophic hosts and microalgae is powered by photosynthesis and relies on the transfer of organic carbon to the host (e.g. sugars). Yet, the identity of transferred carbohydrates as well as the molecular mechanisms that drive this exchange remain largely unknown, especially in unicellular photosymbioses that are widespread in the open ocean. Combining genomics, single-holobiont transcriptomics, and environmental metatranscriptomics, we revealed the transportome of the marine microalga Phaeocystis in symbiosis within acantharia, with a focus on sugar transporters. At the genomic level, the sugar transportome of Phaeocystis is comparable to non-symbiotic haptophytes. By contrast, we found significant remodeling of the expression of the transportome in symbiotic microalgae compared to the free-living stage. More particularly, 36% of sugar transporter genes were differentially expressed. Several of them, such as GLUTs, TPTs, and aquaporins, with glucose, triose-phosphate sugars, and glycerol as potential substrates, were upregulated at the holobiont and community level. We also showed that algal sugar transporter genes exhibit distinct temporal expression patterns during the day. This reprogramed transportome indicates that symbiosis has a major impact on sugar fluxes within and outside the algal cell, and highlights the complexity and the dynamics of metabolic exchanges between partners. This study improves our understanding of the molecular players of the metabolic connectivity underlying the ecological success of planktonic photosymbiosis and paves the way for more studies on transporters across photosymbiotic models.

Environmental Sciences & Ecology↗
DOE OSTI2021

Unbalancing Symbiotic Nitrogen Fixation: Can We Make Effectiveness More Effective?

One of the most critical uses of carbon fuels is in generating nitrogen fertilizer. Atmospheric dinitrogen is too stable to be used directly by plants, so plants need other chemical forms of nitrogen. Nitrogen fertilizer production uses ~4% of the world’s natural gas. Making N fertilizers leverages methane’s energy content by enabling additional energy to be captured into food and fiber through increased photosynthesis. Manufacturing fertilizer is essential—without it there would only be enough combined nitrogen to feed ~3 billion of the world’s ~7 billion people. How to alter this addiction is not obvious, but the best chance to secure substantial and sustainable amounts of future N might be through symbiotic nitrogen fixation (SNF). SNF describes mutualistic interactions where nitrogen-fixing bacteria provide fixed nitrogen to their plant hosts, freeing them from the need for nitrogen fertilizers. SNF already occurs on a large scale; "biological nitrogen” contributes about 5 Tg N/yr to American agriculture, a quarter of the nitrogen needed. Improving SNF and replacing inorganic fertilizers are both important goals in establishing sustainable and more energy-efficient farming. SNF has been used in agriculture for millennia, largely by using legumes like beans or alfalfa in mixed cropping systems. Legume SNF occurs in root nodules, organs that develop after a growing root is infected by bacteria called rhizobia. In the plant cells of a legume nodule, specialized forms of rhizobia fix nitrogen and can produce enough ammonia to supply the growing plant. The exchange of photosynthetically derived plant carbon compounds for nitrogen reduced by the bacteria is the engine that drives the symbiosis. Important details about the organization of development and metabolism in SNF remain to be determined, such as how plant and bacterial metabolism are coordinated as nitrogen is being fixed and what governs the overall level of nitrogen fixation. Most bacterial mutations that affect SNF completely block fixation (e.g. are Fix – ), providing a limited window into the feedback interactions that must be part of the process. We have investigated an unusual mutant of Sinorhizobium meliloti, a symbiotic partner of alfalfa, that fixes dinitrogen at a normal rate (i.e., it is Fix + ) but is not effective in supporting plant growth (i.e., it is ineffective or Eff – ). We have shown that the mutant has a specific mutation in the glnD gene, which codes for a protein that regulates the bacterial response to nitrogen stress, among other key pathways. A Fix + Eff – phenotype contains an unavoidable puzzle—how is it possible for the bacteria to fix nitrogen at a normal rate and without benefiting the nitrogen starved plant host? After eliminating other possibilities, we proposed that the bacteria synthesize a nitrogen-containing compound that the plant can’t use so that acquiring this compound does not relieve plant nitrogen stress. Extracts of nodules made by glnD mutant strains have high concentrations of pyruvate canaline oxime (PCO), a derivative of the plant defensive compound canaline, a toxic analog of ornithine, an amino acid. Many legumes produce canaline and the related arginine mimic canavanine. For reasons we do not yet understand, the mutation in glnD appears to trigger significant overproduction of canaline in a defense response that leads to substantial synthesis of PCO. We expected the bacteria to be able to use PCO, but we have not been able to grow S. meliloti on PCO under several conditions. PCO thus appears to be a metabolic dead end for both the plant and bacteria. We also examined bacterial catabolic pathways that degrade arginine and canavanine and showed that mutation of these did not alter the symbiosis in an obvious way. In related experiments to examine the metabolic development of root nodules, we carried out an ambitious experiment to simultaneously measure metabolites and protein changes during nodule maturation. For these experiments we used a related symbiotic interaction between S. medicae and Medicago truncatula. M. truncatula has a simpler genome than alfalfa so we could identify plant proteins based on predictions from the DNA sequence. Nodules formed on Medicago grow linearly, with the least mature tissues at the tip. Because development of nitrogen fixation is accompanied by the production of the pigmented plant oxygen carrier protein leghemoglobin, we were able to locate and manually separate the tip, transitional, and mature nitrogen-fixing regions from each other. Advances in proteomic and metabolomic techniques allowed us to analyze these tissues from a pool of as few as 10 nodules. We observed correlated transitions of various enzymes in the plant and bacterial proteomes from those characteristic of free-living metabolism to the microaerobic metabolism of the nitrogen-fixing tissues. The metabolome could not be separated into plant and bacterial domains but was consistent with this overall transition. A subsequent paper examined proteolysis in nodule bacteria. Protein turnover in mature regions of the nodule is significant. We were able to show complexes between various proteins and important proteases, using precipitation capture techniques to isolate the proteases and sensitive proteomic analysis to reveal the associated proteins.

09 BIOMASS FUELS↗
DOE OSTI2023

A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities

Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the culture method and whole-genome sequencing were performed. Natural product analyses were carried out to determine the biosynthetic potential. We report the isolation and identification of a symbiotic bacterium Serratia myotis L7-1, that is highly adaptive and resides within Antarctic fish, Trematomus bernacchii . As revealed by genomic analyses, Antarctic strain S. myotis L7-1 possesses carbohydrate-active enzymes (CAZymes), biosynthetic gene clusters (BGCs), stress response genes, antibiotic resistant genes (ARGs), and a complete type IV secretion system which could facilitate competition and colonization in the extreme Antarctic environment. The identification of microbiome gene clusters indicates the biosynthetic potential of bioactive compounds. Based on bioactivity-guided fractionation, serranticin was purified and identified as the bioactive compound, showing significant antibacterial and antitumor activity. The serranticin gene cluster was identified and located on the chrome. Furthermore, the multidrug resistance and strong bacterial antagonism contribute competitive advantages in ecological niches. Our results highlight the existence of a symbiotic bacterium in Antarctic fish largely represented by bioactive natural products and the adaptability to survive in the fish living in Antarctic oceans.

Xiao, Yu↗
DOE OSTI2020

A Mystery in Chamaeleon: Serendipitous Discovery of a Galactic Symbiotic Nova

We present the serendipitous discovery of a low optical-luminosity nova occurring in a D-type symbiotic binary star system in the Milky Way. We lay out the extensive archival data alongside new follow-up observations related to the stellar object CN Cha in the constellation of Chamaeleon. The object had long period (250 days), high amplitude (3 mag) optical variability in its recent past, preceding an increase in optical brightness by 8 magnitudes and a persistence at this brightness for about 3 yr, followed by a period of 1.4 mag yr{sup −1} dimming. The object’s current optical luminosity seems to be dominated by Hα emission, which also exhibits blueshifted absorption (a P-Cygni-like profile). After consideration of a number of theories to explain these myriad observations, we determine that CN Cha is most likely a symbiotic (an evolved-star–white-dwarf binary) system that has undergone a long-duration, low optical brightness, nova, placing it squarely in the class of so-called “slow novae,” of which there are only a few known examples. The duration of the optical plateau in CN Cha would make it the shortest timescale plateau of any known slow symbiotic novae.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1981

Ingress observations of the 1980 eclipse of the symbiotic star CI Cyngni

One of the major results from the IUE may prove to be the knowledge gained by studies of the ultraviolet spectra of symbiotic stars. Symbiotics combine spectral features of a cool M giant like photosphere with strong high excitation emission lines of nebular origin, superposed. The UV spectra are dominated by intense permitted and semiforbidden emission lines and weak continua indicative of hot compact objects and accretion disks. Two symbiotics, AR Pav and CI Cyg are thought to be eclipsing binaries and IUE observations during the 1980 eclipse of CI Cygni are discussed.

Stencel, R. E.↗
NASA NTRS1982

Ultraviolet properties of the symbiotic stars

A general discussion of the UV spectra of symbiotic stars, including both the emission lines and the continua, is presented, with AG Pegasi considered as an illustrative example. It is noted that the IUE observations of the symbiotics have revealed UV properties which rival the diversity of the optical features. Nevertheless, the UV data have for the first time permitted the hot component to be studied relatively uncontaminated by the giant companion, which dominates the optical regime. The UV observations provide convincing evidence that many of the symbiotics have hot stellar companions embedded in the enshrouding nebula or accretion shell formed from the wind from one or possibly both of the components.

Slovak, M. H.↗
NASA NTRS1984

ZZ Canis Minoris as a symbiotic star

The H-aplha and Na I D-line regions of the M6 giant star ZZ Canis Minoris (ZZ CMi) were observed with the Kitt Peak coude feed telescope and a CCD detector. It is shown that ZZ CMi has similar spectroscopic and photoproperties to the symbiotic star EG And. The data are used to argue for the classification of ZZ CMi as a symbiotic star despite its current listing in the General Catalog of Variable Stars (GCVS) as a semi-regular variable. The infrared magnitudes of ZZ CMi and the known symbiotic stars are compared in a table.

Bopp, B. W.↗
NASA NTRS1987

The (C III lambda 1909/Si III lambda 1892) ratio as a diagnostic for planetary nebulae and symbiotic stars

Suitable IUE archival material on planetary nebulae has been examined to determine the log R /F(lambda 1909 C III)/F(lambda 1892 Si III)/ as a discriminant for distinguishing planetary nebulae from symbiotic stars and related objects. The mean value of log R for 73 galactic planetaries is 1.4, while that of extragalactic planetaries appears to be slightly lower, and that for symbiotics is 0.3. The lower value of log R for symbiotics is easily understood as a consequence of their higher densities. A plot of log R versus N-epsilon indicates that 80 percent of the planetaries fall into the range of log R between 1.2 and 1.8, but some of the 'peculiar' and bipolar nebulae fall below log R = 1.2. The corresponding N(C++)/N(Si++) ionic ratio varies over a large range.

Feibelman, Walter A.↗
NASA NTRS1988

Far-infrared data for symbiotic stars. II - The IRAS survey observations

IRAS survey data for all known symbiotic binaries are reported. S type systems have 25 micron excesses much larger than those of single red giant stars, suggesting that these objects lose mass more rapidly than do normal giants. D type objects have far-IR colors similar to those of Mira variables, implying mass-loss rate of about 10 to the -6th solar masses/yr. The near-IR extinctions of the D types indicate that their Mira components are enshrouded in optically thick dust shells, while their hot companions lie outside the shells. If this interpretation of the data is correct, then the very red near-IR colors of D type symbiotic stars are caused by extreme amounts of dust absorption rather than dust emission. The small group of D prime objects possesses far-IR colors resembling those of compact planetary nebulae or extreme OH/IR stars. It is speculated that these binaries are not symbiotic stars at all, but contain a hot compact star and an exasymptotic branch giant which is in the process of ejecting a planetary nebula shell.

Kenyon, S. J.↗
NASA NTRS1988

The C IV doublet ratio intensity effect in symbiotic stars

High-resolution UV spectra in the 1200-2000 wavelength range of the symbiotic variable R Aqr and its nebular jet were obtained in July 1987 with the IUE. The line profile structure of the C IV 1548, 1550 doublet in the jet indicates multicomponent velocity structure from an optically thin emitting gas. The C IV doublet profiles in the compact H II region engulfing the Mira and hot companion binary also suggest multicomponent structure with radial velocities up to about -100 km/s. The value of the doublet intensity ratio in the R Aqr H II region has been observed in other similar symbiotic stars, such as RX Pup. It is suggested that the anomalous behavior of the C IV doublet intensities may be useful for studying the spatial structure and temporal nature of winds in symbiotic stars.

Michalitsianos, A. G.↗
NASA NTRS1987

Dynamic task allocation for a man-machine symbiotic system

This report presents a methodological approach to the dynamic allocation of tasks in a man-machine symbiotic system in the context of dexterous manipulation and teleoperation. This report addresses a symbiotic system containing two symbiotic partners which work toward controlling a single manipulator arm for the execution of a series of sequential manipulation tasks. It is proposed that an automated task allocator use knowledge about the constraints/criteria of the problem, the available resources, the tasks to be performed, and the environment to dynamically allocate task recommendations for the man and the machine. The presentation of the methodology includes discussions concerning the interaction of the knowledge areas, the flow of control, the necessary communication links, and the replanning of the task allocation. Examples of task allocation are presented to illustrate the results of this methodolgy.

Parker, L. E.↗
NASA NTRS1994

A far-ultraviolet atlas of symbiotic stars observed with IUE. 1. The SWP range

This atlas contains sample spectra from the far-ultraviolet observations of 32 symbiotic stars obtained with the International Ultraviolet Explorer (IUE) satellite. In all, 394 low-resolution spectra from the short-wavelength primary (SWP) camera covering the range 1200-2000 A have been extracted from the IUE archive, calibrated, and measured. Absolute line fluxes and wavelengths for the prominent emission lines have been tabulated. Tables of both the general properties of these symbiotics and of features specific to the spectrum of each are included. The spectra shown are representative of the different classes of symbiotic stars that are currently in the IUE archive. These include known eclipsing systems and those that have been observed in outburst (as well as quiescence).

Meier, S. R.↗
NASA NTRS1993

The onset of Wolf-Rayet wind outflow and the nature of the hot component in the symbiotic nova PU Vulpecula

We have analyzed temporal variations in the far ultraviolet He II (1640), Si IV (1393, 1402), and C IV (1548, 1550) line profiles in eight high dispersion, International Ultraviolet Explorer Short Wavelength Prime spectra of the symbiotic nova PU Vul by comparatively examining these profiles on a common velocity scale. We see clear evidence of the onset of a Wolf-Rayet-like wind outflow from the bloated, contracting white dwarf hot component with terminal velocity of approximately equals -550 to -600 km/s. We have quantitatively analyzed the complicated He II (1640) emission region for the first time and show that the discrete absorption features seen in the He II region occur at precisely the same velocites in each spectrum, thus demonstrating that the absorbing source is steady and not affected by any orbital motion. We demonstrate that there is an underlying He II wind emission feature whose true shape is hidden by superposed absorption due to the foreground red giant wind flowing in front of the white dwarf and abscuring the white dwarf's wind outflow. We present synthetic spectra of He II emission behind an absorbing slab with u = 20 km/s, T = 5000 K, and column densities in the range N = 1 x 10(exp 22) and 1 x 10(exp 23)/sq cm which explain these absorptions. Our analysis of the Si IV and C IV resonance doublets, in velocity space, reveal temporal variations in the profile between 1987 and 1991 with the emergence of clear P Cygni profiles in Si IV by 1990. A nebular emission feature in C III 1909 also appears in the most recent spectra (e.g., SW42538H) while it was absent or extremely weak in the earliest spectra (e.g., SW36332H), thus strengthening evidence that the nebular emission, as seen in permitted and semiforbidden lines, intensities in step with the onset of the hot, fast, wind outflow. We also report the first detection of narrow interstellar (circumbinary shell?) absorption lines near -1 km/s, most strongly in Al III (1854, 1862) and Si IV (1392, 1402). We have carried out a rough quantitative analysis of the He II wind emission by using the theoretical He II Wolf-Rayet profiles of Hamann & Schmutz (1987). We obtain a lower limit to the He II net emission equivalent width of approximately 1 A, a hot component temperature in the range 25,000 to 35,000 K, a hot component radius in the range 5 solar radius to 30 solar radius, a maximum wind velocity of approximately equals -600 km/s and a rough upper limit to the mass-loss rate of less than 1 x 10(exp -5) solar mass/yr. To our knowledge, this is the first quantitative wind analysis, albeit crude, to be carried out for the hot component of a symbiotic nova or symbiotic variable.

Sion, Edward M.↗
NASA NTRS2007

The Symbiotic System SS73 17 seen with Suzaku

We observed with Suzaku the symbiotic star SS73 17, motivated by the discovery by the INTEGRAL satellite and the Swift BAT survey that it emits hard X-rays. Our observations showed a highly-absorbed X-ray spectrum with NH > loz3 emp2, equivalent to Av > 26, although the source has B magnitude 11.3 and is also bright in UV. The source also shows strong, narrow iron lines including fluorescent Fe K as well as Fe xxv and Fe XXVI. The X-ray spectrum can be fit with a thermal model including an absorption component that partially covers the source. Most of the equivalent width of the iron fluorescent line in this model can be explained as a combination of reprocessing in a dense absorber plus reflection off a white dwarf surface, but it is likely that the continuum is partially seen in reflection as well. Unlike other symbiotic systems that show hard X-ray emission (CH Cyg, RT Cru, T CrB, GX1+4), SS73 17 is not known to have shown nova-like optical variability, X-ray flashes, or pulsations, and has always shown faint soft X-ray emission. As a result, although it is likely a white dwarf, the nature of the compact object in SS73 17 is still uncertain. SS73 17 is probably an extreme example of the recently discovered and relatively small class of hard X-ray emitting symbiotic systems.

Smith, Randall K.↗
NASA NTRS2014

Symbiotic Stars in X-rays. II. Faint Sources Detected with XMM-Newton and Chandra

We report the detection from four symbiotic stars that were not known to be X-ray sources. These four object show a β-type X-ray spectrum, that is, their spectra can be modeled with an absorbed optically thin thermal emission with temperatures of a few million degrees. Photometric series obtained with the Optical Monitor on board XMM-Newton from V2416 Sgr and NSV 25735 support the proposed scenario where the X-ray emission is produced in a shock-heated region inside the symbiotic nebulae.

binaries: symbiotic aEuro" X-rays: binaries↗
DOE OSTI2025

Mechanistic Insights into Visible-Light-Induced ATRA Reactions Powered by the Symbiotic Relationship between Cu(II)/Cu(I) Phenanthroline Complexes

As photoredox catalysis continues to yield promising chemical transformations, there is an increased need to understand how specific photocatalysts function to improve reaction efficiencies while expanding their scope. Copper-phenanthroline-based photocatalysts such as Cu II (dap)Cl 2 (dap = 2,9-di(p-anisyl)-1,10-phenanthroline) and [Cu I (dap) 2 ]Cl were both found to be equally capable of olefin activation through electrophilic atom transfer radical addition (ATRA) reactions. Although these molecular catalysts have proven successful, many intermediates suggested in the proposed catalytic cycles have never been detected. One undetermined aspect in this chemistry is related to how one equivalent of Cu II (dap)Cl 2 generates half of an equivalent of [Cu I (dap) 2 ] + during the photocatalytic sequence. To this end, we initially used more synthetically accessible model systems, namely, [Cu I (dpp) 2 ]Cl and Cu II (dpp)Cl 2 (dpp = 2,9-diphenyl-1,10-phenanthroline), to glean detailed mechanistic insights into this unusual symbiotic relationship. We directly detected several intermediates involved in the ATRA photocatalytic cycle using these model chromophores in conjunction with electronic spectroscopy, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry, electronic structure calculations, EPR spin-trap experiments, and 1 H NMR spectroscopy. We found that the unique ligand lability and coordinating properties of acetonitrile enable both the in situ oxidation of [Cu I (dpp) 2 ] + by tosyl chloride into Cu II (dpp)Cl 2 and the visible-light-induced homolysis of the Cu II –Cl bond, which initiates the conversion to the Cu I species [Cu I (dpp) 2 ][Cu I Cl 2 ]. Here, the combined findings from the present study of the catalytic cycle demonstrate that the symbiotic relationship between Cu II (dpp)Cl 2 and [Cu I (dpp) 2 ] + , as well as between Cu II (dap)Cl 2 and [Cu I (dap) 2 ] + , is the critical factor enabling the ATRA photoreaction by departing from either photocatalyst.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗