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NASA NTRS2017

Vascular Patterning Analysis by VESGEN 2D/3D with Bioinformatics: Updates for Rodent Tissues

Fractally branching vascular systems are a complex physiological requirement shared by humans with all higher terrestrial life forms, including other vertebrates, insects, and higher land plants. Vascular trees, networks, and tree-network composites are therefore mapped and quantified by the VESsel GENeration Analysis (VESGEN) software according to weighted physiological vascular rules that include vessel connectivity, tapering and bifurcational branching. According to fluid dynamics, successful vascular transport depends upon a complex distributed system of highly regulated laminar flow. VESGEN has elucidated changes in vascular patterning resulting from inflammatory, developmental and other signaling pathways within numerous tissues of major model organisms important for Space Biology, especially for rodents. Important early stage regenerative opportunities have been identified by VESGEN vascular analysis for visual impairments in the human retina, and is currently being used for research into astronaut visual and ocular disorders associated with long duration missions. The VESGEN 2D software is a mature, automated, widely published capability for which beta testing and public release by NASA is planned for the upcoming year. Early-stage capabilities for VESGEN 3D analysis are under development for the rodent retina and intestine as prototype tissues. A prototype VESGEN 2D Bioinformatics software capability has also been developed to associate phenotypic changes in molecular expression with vascular structure and function. By new VESGEN bioinformatic innovations, expression patterns of the genetic, transcriptional, protein and other markers for regulatory molecules such as vascular endothelial growth factor (VEGF) and their receptors, often indicators of tissue oxygenation status, are co-localized with alterations in vascular pattern. Biomarkers are therefore mapped and quantified as information dimensions directly correlated with the spatial dimensions of a vascular pattern. Further important technology innovations by NASA include substantial image segmentation advances for more automated binary extraction of the grayscale vascular patterns, together with informative associated image quality assessments. Vascular mapping and quantification capabilities for the rodent retina and intestine are illustrated for VESGEN 2D, along with technology status reports on VESGEN 3D and Bioinformatic capabilities. Research partially supported by Ames Center Innovation Awards.

Parsons-Wingerter, P.↗
NASA NTRS1992

Monitoring environmental state of Alaskan forests with AIRSAR

During March 1988 and May 1991, the JPL airborne synthetic aperture radar, AIRSAR, collected sets of multi-temporal imagery of the Bonanza Creek Experimental Forest near Fairbanks, Alaska. These data sets consist of series of multi-polarized images collected at P-, L-, and C-bands each over a period of a few days. The AIRSAR campaigns were complemented with extensive ground measurements that included observations of both static canopy characteristics such as forest architecture as well as properties that vary on short term time scales such as canopy dielectric conditions. Observations exist for several stands of deciduous and coniferous species including white spruce (Picea glauca), black spruce (Picea mariana), and balsam poplar (Populus balsamifera). Although the duration of each campaign was fairly short, significant changes in environmental conditions caused notable variations in the physiological state of the canopies. During the 1988 campaign, environmental conditions ranged from unseasonably warm to more normal subfreezing temperatures. This permitted AIRSAR observations of frozen and thawed canopy states. During May 1991, ice jams that occurred along the river caused many stands to flood while the subsequent clearing of the river then allowed the waters to recede, leaving a snow covered ground surface. This allowed observations of several stands during both flooded and nonflooded conditions. Furthermore, the local weather varied from clear sunny days to heavy overcast days with some occurrence of rain. Measurements of leaf water potential indicated that this caused significant variations in canopy water status, allowing SAR observations of water stressed and unstressed trees. Mean backscatter from several stands is examined for the various canopy physiological states. The changes in canopy backscatter that occur as a function of environmental and physiological state are analyzed. Preliminary results of a backscatter signature modeling analysis are presented. The implications of using SAR to monitor canopy phenological state are addressed.

Mcdonald, Kyle C.↗
NASA NTRS2017

VESsel GENeration Analysis (VESGEN): Innovative Vascular Mappings for Astronaut Exploration Health Risks and Human Terrestrial Medicine

Currently, astronauts face significant health risks in future long-duration exploration missions such as colonizing the Moon and traveling to Mars. Numerous risks include greatly increased radiation exposures beyond the low earth orbit (LEO) of the ISS, and visual and ocular impairments in response to microgravity environments. The cardiovascular system is a key mediator in human physiological responses to radiation and microgravity. Moreover, blood vessels are necessarily involved in the progression and treatment of vascular-dependent terrestrial diseases such as cancer, coronary vessel disease, wound-healing, reproductive disorders, and diabetes. NASA developed an innovative, globally requested beta-level software, VESsel GENeration Analysis (VESGEN) to map and quantify vascular remodeling for application to astronaut and terrestrial health challenges. VESGEN mappings of branching vascular trees and networks are based on a weighted multi-parametric analysis derived from vascular physiological branching rules. Complex vascular branching patterns are determined by biological signaling mechanisms together with the fluid mechanics of multi-phase laminar blood flow.

astronaut↗
NASA NTRS2009

Cambium

The Cambium investigation is one in a pair of investigations which utilizes the Advanced Biological Research System (ABRS). Cambium seeks definitive evidence that gravity has a direct effect on cambial cells (cells located under the inner bark where secondary growth occurs) in willow, Salix babylonica. The Cambium investigation uses willow plants flown on the International Space Station to better understand the fundamental processes by which plants produce cellulose and lignin, the two main structural materials found in plant matter. On Earth, the nature of wood within tree stems varies depending on position, and that within-tree variation includes differences in cell types and chemistry including lignin and cellulose, two major components of wood influencing wood strength, usefulness and carbon content. Reaction wood is an extreme example of such variation, and it is believed that reaction wood develops as a reaction to the influence of gravity. For the Cambium experiment, young willow plants will be launched to the ISS where their stems will be looped in an attempt to induce reaction wood formation. After on-orbit growth, the plants will be preserved and returned to Earth for analysis. Understanding the role of gravity in wood formation is expected to enable wiser management of forests for carbon sequestration as well as better utilization of trees for wood products. Detailed Research Description: The Cambium experiment will provide an understanding of physiological processes such as gene expression, metabolism and general plant development that are affected in plant systems exposed to space flight. Cambium seeks definitive evidence that gravity has a direct effect on the cambial cells (cells located under the inner bark where secondary growth occurs) that contribute to xylogenesis (reaction wood formation) in willow plants, Salix babylonica. Tension wood fibers differentiate on the upper sides of stems when the stem is altered from its normal (vertical) growth position by looping. This reaction wood response does not occur if the orientation of the plant stem with respect to gravity is not altered. If a localized zone of tension wood should be formed in looped stems under microgravity conditions, this would be the first conclusive evidence that gravity is not required. On the other hand, if a zone of tension wood is not produced in looped stems (subjected to tension on one side, compression on the other) in microgravity, this would be the first definitive evidence that gravity has a direct effect on the cambial cells which contribute to reaction wood formation. Following return to Earth the plants will be analyzed by microscopy and chemical methods.

Savidge, Rodney↗
NASA NTRS1998

Diurnal Reflectance Changes in Vegetation Observed with AVIRIS

Among the most important short-term dynamic biological processes are diurnal changes in canopy water relations. Plant regulation of water transport through stomatal openings affects other gaseous transport processes, often dramatically decreasing photosynthetic fixation of carbon dioxide during periods of water stress. Water stress reduces stomatal conductance of water vapor through the leaf surface and alters the diurnal timing of stomatal opening. Under non-water stressed conditions, stomates typically open soon after dawn and transpire water vapor throughout the daylight period. During stress periods, stomates may close for part of the day, generally near mid-day. Under prolonged stress conditions, stomatal closure shifts to earlier times during the day; stomates may close by mid-morning and remain closed until the following morning - or remain closed entirely. Under these conditions the relationship between canopy greenness (e.g., measured with a vegetation index or by spectral mixture analysis) and photosynthetic fixation of carbon is lost and the remotely sensed vegetation metric is a poor predictor of gas exchange. Prediction of stomatal regulation and exchange of water and trace gases is critical for ecosystem and climate models to correctly estimate budgets of these gases and understand or predict other processes like gross and net ecosystem primary production. Plant gas exchange has been extensively studied by physiologists at the leaf and whole plant level and by biometeorologists at somewhat larger scales. While these energy driven processes follow a predictable if somewhat asymmetric diurnal cycle dependent on soil water availability and the constraints imposed by the solar energy budget, they are nonetheless difficult to measure at the tree and stand levels using conventional methods. Ecologists have long been interested in the potential of remote sensing for monitoring physiological changes using multi-temporal images. Much of this research has focused on day-to-day changes in water use, especially for agricultural applications. Ustin et al. showed seasonal changes in canopy water content in chaparral shrub could be estimated using optical methods. Vanderbilt et al. followed asymmetric diurnal changes in the reflectance of a walnut orchard, but could not attribute specific reflectance changes to specific changes in canopy architecture or physiology. Forests and shrub lands in California experience prolonged periods of drought, sometimes extending six months without precipitation. The conifer and evergreen chaparral communities common to the foothill region around the central valley of California retain their foliage throughout the summer and have low transpiration rates despite high net radiation and temperature conditions. In contrast, grasslands and drought resistant deciduous species in the same habitat are seasonally dormant in summer. Because of differences in the mechanisms of drought tolerance, rooting depth and physiology between different plant communities in the region, it is likely that they display differences in diurnal water relations. The presence of diverse plant communities provides an opportunity to investigate possible diurnal landscape patterns in water relations that could be observed by an airborne hyperspectral scanner. This investigation of AVIRIS data collected over forest and shrub land represents the continuation of a prior investigation involving spectral mixture analysis of diurnal effects in the same AVIRIS data set.

Vanderbilt, V. C.↗
NASA NTRS1999

Leaf Optical Properties in Higher Plants: Linking Spectral Characteristics with Plant Stress

A number of studies have addressed responses of leaf spectral reflectance, transmittance, or absorptance to physiological stress. Stressors included dehydration, ozone, herbicides, disease, insufficient mycorrhizae and N fertilization, flooding and insects. Species included conifers, grasses, and broadleaved trees. Increased reflectance with maximum responses near 700 nm wavelength occurred in all cases. Varying the chlorophyll content in leaves or pigment extracts can simulate this effect. Thus, common optical responses to stress result from decreases in leaf chlorophyll contents or the capacity of chloroplasts to absorb light. Leaf optic can be quite sensitive to any stressor that alters soil-plant-atmosphere processes.

Carter, Gregory A.↗
NASA NTRS2016

Fractal Branching in Vascular Trees and Networks by VESsel GENeration Analysis (VESGEN)

Vascular patterning offers an informative multi-scale, fractal readout of regulatory signaling by complex molecular pathways. Understanding such molecular crosstalk is important for physiological, pathological and therapeutic research in Space Biology and Astronaut countermeasures. When mapped out and quantified by NASA's innovative VESsel GENeration Analysis (VESGEN) software, remodeling vascular patterns become useful biomarkers that advance out understanding of the response of biology and human health to challenges such as microgravity and radiation in space environments.

fractal↗
NASA NTRS1970

The development of spectro-signature indicators of root disease impacts on forest stands

A field research program was begun in 1969 and intensified in 1970 on the physiology and biophysical responses of second-growth Douglas fir infected with root rot fungus. A double tramway system was suspended between three 100-foot instrument towers to carry sensors for measuring the energy response from above both healthy and infected trees. Processing and analysis was completed of airborne multispectral scanner imagery collected over the Wind River research area in 1969. Likelihood ratio processing of three-channel infrared data and Euclidean distance analysis of ten-channel spectrometer data did not identify incipient root rot infection outside the training sets. In all cases infected fir was misclassified as healthy fir. It was concluded from careful examination of physiological data that Poria root rot infection has little effect on water metabolism and energy exchange. What was identified was a low-grade stress that affects respiration and metabolism over long periods of time. This led to minor changes in the external physical symptoms of Poria-infected trees which was revealed only in the shortwave reflectance data.

Weber, F. P.↗
NASA NTRS1986

A simple biosphere model (SiB) for use within general circulation models

A simple realistic biosphere model for calculating the transfer of energy, mass and momentum between the atmosphere and the vegetated surface of the earth has been developed for use in atmospheric general circulation models. The vegetation in each terrestrial model grid is represented by an upper level, representing the perennial canopy of trees and shrubs, and a lower level, representing the annual cover of grasses and other heraceous species. The vegetation morphology and the physical and physiological properties of the vegetation layers determine such properties as: the reflection, transmission, absorption and emission of direct and diffuse radiation; the infiltration, drainage, and storage of the residual rainfall in the soil; and the control over the stomatal functioning. The model, with prescribed vegetation parameters and soil interactive soil moisture, can be used for prediction of the atmospheric circulation and precipitaion fields for short periods of up to a few weeks.

Sellers, P. J.↗
NASA NTRS1993

Entropy reduction via simplified image contourization

The process of contourization is presented which converts a raster image into a set of plateaux or contours. These contours can be grouped into a hierarchical structure, defining total spatial inclusion, called a contour tree. A contour coder has been developed which fully describes these contours in a compact and efficient manner and is the basis for an image compression method. Simplification of the contour tree has been undertaken by merging contour tree nodes thus lowering the contour tree's entropy. This can be exploited by the contour coder to increase the image compression ratio. By applying general and simple rules derived from physiological experiments on the human vision system, lossy image compression can be achieved which minimizes noticeable artifacts in the simplified image.

Turner, Martin J.↗
NASA NTRS2018

Differences in Pre and Post Vascular Patterning Within Retinas from ISS Crew Members and Head-Down Tilt (HDT) Subjects by VESGEN Analysis

Accelerated research by NASA has investigated the significant risks incurred during long-duration missions in microgravity for Space Flight-Associated Neuro-ocular Syndrome (SANS, formerly known as Visual Impairments associated with Increased Intracranial Pressure, VIIP) [1]. For our study, NASA's VESsel GENeration Analysis (VESGEN) was used to investigate the role of retinal blood vessels in the etiology of SANS/VIIP. The response of retinal vessels to microgravity was evaluated in astronaut crew members pre and post flight to the International Space Station (ISS), and compared to the response of retinal vessels in healthy volunteers to 6deg head-down tilt during 70 days of bed rest (HDTBR). For the study, we are testing the hypothesis that long-term cephalad fluid shifts resulting in ocular and visual impairments are necessarily mediated in part by retinal blood vessels, and therefore are accompanied by structural adaptations of the vessels. METHODS: Vascular patterns in the retinas of crew members and HDTBR subjects extracted from 30deg infrared (IR) Heidelberg Spectralis images collected pre/postflight and pre/post HDTBR, respectively, were analyzed by VESGEN (patent pending). VESGEN is a mature, automated software developed as a research discovery tool for progressive vascular diseases in the retina and other tissues. The multi-parametric VESGEN analysis generates maps of branching arterial and venous trees quantified by parameters such as the fractal dimension (Df, a modern measure of vascular space-filling capacity), vessel diameters, and densities of vessel length and number classified into specific branching generations according to vascular physiological branching rules. The retrospective study approved by NASA's Institutional Review Board included the analysis of bilateral retinas in eight ISS crew members monitored by routine occupational surveillance and six HDTBR subjects (NASA FARU Campaign 11, for example). The VESGEN analysis was conducted in a blinded fashion, with IR retinal images masked to the subject's identity, ophthalmic and clinical characteristics, and to the temporal sequence of image collection. To complete our study, VESGEN results will be analyzed statistically and correlated with other ophthalmic and medical findings. RESULTS: Preliminary results for changes in the pre to post status of vascular patterning in the retinas of crew members and HDTBR subjects are interestingly opposite. By Df and other vascular branching measures, the space-filling capacity of arterial and venous trees decreased in the majority of crew members (11/16 retinas). In contrast, vascular densities increased in HDTBR subjects by the same parameters (6/10 retinas). To conclude the study, biostatistics and medical analyses will be conducted to quantify and draw conclusions about how the changes associated with flight compare to those associated with HDTBR. CONCLUSIONS: Vascular densities appeared to decrease in the retinas of ISS crew members and increase in HDTBR subjects. Differences in arterial and venous response to cephalad fluid shifts induced by ISS and HDTBR may have resulted from a long-duration conditioning phenomenon (for example, 6-month ISS missions compared to 70 days HDTBR), or the presence of gravity in HDTBR compared to microgravity on the ISS. In addition, increased and decreased vessel diameters for Crew Members and HDTBR, respectively, are subject to limits of im

Vascular↗
NASA NTRS2018

Differences in Pre and Post Vascular Patterning of Retinas from ISS Crew Members and HDT Subjects by VESGEN Analysis

Accelerated research by NASA [1] has investigated the significant risks for visual and ocular impairments Spaceflight Associated Neuro-Ocular Syndrome /Visual Impairment/Intracranial Pressure (SANS/VIIP) incurred by microgravity spaceflight, especially long-duration missions. Our study investigates the role of blood vessels in the incidence and etiology of SANS/VIIP within the retinas of Astronaut crewmembers pre-and post-flight to the International Space Station (ISS) by NASA's VESsel GENeration Analysis (VESGEN). The response of retinal vessels in crewmembers to microgravity was compared to that of retinal vessels to Head-Down Tilt (HDT) in subjects undergoing 70-Day Bed Rest. The study tests the proposed hypothesis that cephalad fluid shifts missions, resulting in ocular and visual impairments, are necessarily mediated in part by retinal blood vessels, and are therefore accompanied by significant remodeling of retinal vasculature.Vascular patterns in the retinas of crew members and HDTBR subjects extracted from 30° infrared (IR) Heidelberg Spectralis® images collected pre/postflight and pre/post HDTBR, respectively, were analyzed by VESGEN (patent pending). a mature, automated software developed as a research discovery tool for progressive vascular diseases in the retina and other tissues [2]. The weighted, multi-parametric VESGEN analysis generates maps of branching arterial and venous trees and quantification by parameters such as the fractal dimension (Df, a modern measure of vascular space-filling capacity), vessel diameters, and densities of vessel length and number classified into specific branching generations by vascular physiological branching rules [2,3]. The retrospective study approved by NASA’s Institutional Review Board included six HDT subjects (NASA Flight Analogs Research Unit [FARU] Campaign 11; for example, [4]) and eight ISS crewmembers monitored by routine occupational surveillance who provided their study consents to NASA’s Lifetime Surveillance of Astronaut Health (LSAH). For the initial blinded VESGEN phase, ophthalmic retinal images were masked as to subject identity and pre- and post-status. In the second unblinded phase, VESGEN results were analyzed according to the pre- and post-status of left and right retinas matched to each subject. To complete our study, vascular results will be subjected to NASA biostatistical analysis and correlated with other ophthalmic and medical findings. Preliminary results for changes in the pre- to post-status of vascular patterning in the retinas of crewmembers and HDT subjects are strikingly opposite. By Df and other vascular branching measures, the space-filling capacity of arterial and venous trees decreased in a substantial subset of crewmembers (11/16 retinas). In contrast, vascular densities increased in a substantial subset of HDT subjects by the same parameters (6/10 retinas, currently excluding one anomalous subject). To conclude the study, biostatistical and medical analyses will be of critical importance for investigating the validity of these vascular findings. Vascular densities appeared to decrease in the retinas of crewmembers following ISS Missions, and increase in subjects after HDT. The vascular increases and decreases most likely derive primarily from limits of resolution to the ophthalmic imaging that does not capture the smallest vessels, rather than from vessel growth or atrophy. Differences in arterial and venous response to cephalad fluid shifts induced by ISS and HDT may have resulted from a long-duration conditioning phenomenon (for example, 6-month ISS missions compared to 70-day HDT), or the presence of gravity in HDT compared to microgravity onboard the ISS. To conclude our study, the biostatistical and medical analyses will be of critical importance for investigating the validity and significance of the VESGEN findings.

Murray, M. C.↗
NASA NTRS2000

Leaf Optical Properties in Higher Plants: Linking Spectral Characteristics to Stress and Chlorophyll Concentration

A number of studies have linked responses in leaf spectral reflectance, transmittance or absorptance to physiological stress. A variety of stressors including dehydration, flooding,freezing, ozone, herbicides, competition, disease, insects and deficiencies in ectomycorrhizal development and N fertilization have been imposed on species ranging from grasses to conifers and deciduous trees. In this cases, the maximum difference in reflectance within the 400 - 850 nm wavelength range between control and stressed states occurred as a reflectance increase at wavelength near 700 nm. In studies that included transmittance and absorptance as well as reflectance, maximum differences occurred as increases and decreases, respectively, near 700 nm. This common optical response to stress could be simulated closely by varying the chlorophyll concentrations in senescent leaves of five species. The optical response to stress near 700 nm, as well as corresponding changes in reflectance that occur in the green-yellow spectrum, can be explained by the general tendency of stress to reduce leaf chlorophyll concentration.

Carter, Gregory A.↗
NASA NTRS1990

Modeling of interactions between biosphere and atmosphere on the global scale

Simulations of weather and climate with general circulation models show that the circulation and rainfall are sensitive to the transfers of radiation, sensible heat, water vapor, and momentum across the atmosphere land surface interface. Because, in nature, these transfers depend in large measure on the morphological and physiological characteristics of the surface vegetation, an interactive biosphere is needed for GCM's. The simple model of the biosphere (SiB) that the authors have constructed is based on the recognized difference between ground cover vegetation (grasses and other herbaceous plants) and canopy vegetation (trees and shrubs), where the latter act like elevated blocks of porous material which efficiently extract momentum from the air that flows through them; by a large number of multiple reflections enhance the absorption of the incident solar radiation; when the canopy is wet, act like well ventilated psychrometers which extract sensible heat from the air and produce a negative Bowen ratio; and, in all of these ways, behave differently from ground cover vegetation. The complete set of governing equations of SiB, whereby the various allowable combinations of trees, ground covers, and bare ground, in interaction with the atmosphere, determine all of the vegetation dependent transfers at the land surface, have been presented and described.

Baer, Ferdinand↗
NASA NTRS1971

Photographic Remote Sensing of Sick Citrus Trees

Remote sensing with infrared color aerial photography (Kodak Ektachrome Infrared Aero 8443 film) for detecting citrus tree anomalies is described. Illustrations and discussions are given for detecting nutrient toxicity symptoms, for detecting foot rot and sooty mold fungal diseases, and for distinguishing among citrus species. Also, the influence of internal leaf structure on light reflectance, transmittance, and absorptance are considered; and physiological and environmental factors that affect citrus leaf light reflectance are reviewed briefly and illustrated.

Gausman, H. W.↗
NASA NTRS2022

Modeling Demographic-Driven Vegetation Dynamics and Ecosystem Biogeochemical Cycling in NASA GISS’s Earth System Model (ModelE-BiomeE v.1.0)

We developed a demographic vegetation model, BiomeE, to improve the modeling of vegetation dynamics and ecosystem biogeochemical cycles in the NASA Goddard Institute of Space Studies' ModelE Earth system model. This model includes the processes of plant growth, mortality, reproduction, vegetation structural dynamics, and soil carbon and nitrogen storage and transformations. The model combines the plant physiological processes of ModelE's original vegetation model, Ent, with the plant demographic and ecosystem nitrogen processes that have been represented in the Geophysical Fluid Dynamics Laboratory's LM3-PPA. We used nine plant functional types to represent global natural vegetation functional diversity, including trees, shrubs, and grasses, and a new phenology model to simulate vegetation seasonal changes with temperature and precipitation fluctuations. Competition for light and soil resources is individual based, which makes the modeling of transient compositional dynamics and vegetation succession possible. Overall, the BiomeE model simulates, with fidelity comparable to other models, the dynamics of vegetation and soil biogeochemistry, including leaf area index, vegetation structure (e.g., height, tree density, size distribution, and crown organization), and ecosystem carbon and nitrogen storage and fluxes. This model allows ModelE to simulate transient and long-term biogeophysical and biogeochemical feedbacks between the climate system and land ecosystems. Furthermore, BiomeE also allows for the eco-evolutionary modeling of community assemblage in response to past and future climate changes with its individual-based competition and demographic processes.

Biogeochemical cycles↗
NASA NTRS2016

Analysis of Retinal Vascular Branching in Human Subjects Undergoing 70-Day Head-Down Tilt by NASAs VESGEN Software

Significant risks for visual impairment were discovered recently in astronauts following spaceflight, especially after long-duration missions. We hypothesize that microgravity-induced fluid shifts result in pathological changes within the retinal vasculature that precede visual and other ocular impairments. We therefore are analyzing retinal vessels in healthy subjects before and after head-down tilt (HDT), a ground-based microgravity analog with NASA's VESsel GENeration Analysis (VESGEN) software. Methods. Spectralis® infrared (IR) fundus images were collected from both eyes of 6 subjects before and after 70 days of bed rest at 6 degree HDT (NASA Campaign 11). For our retrospective study, branching patterns in arterial and venous trees are mapped by VESGEN into vessel branching generations (Gx) that are quantified by parameters such as densities of vessel length (Lv), area (Av), number (Nv) and fractal dimension (Df) as described previously for diabetic retinopathy (IOVS 51(1):498). Results are further assigned by VESGEN into groups of large (G1-3), medium (G4-6) and small (G≥7) vessels. Results. All subjects remained asymptomatic throughout duration of HDT. To date, we have analyzed one IR image from each of the 12 eyes. Interestingly, two groups of the masked study population identified by VESGEN are distinguished by the presence or absence of small veins (G≥7). For example, L≥7 and Av≥7 are 2.7+/-1.3 E-4 px/px2 and 7.2+/-3.6 E-4 px2/px2 in 6 retinas, but 0 in the other 6 retinas. Nonetheless, the space-filling properties of the entire venous trees were remarkably uniform by all parameters, such as Df = 1.56+/-0.02 for 6 retinas with G≥7 and 1.55+/-0.02 for retinas without G≥7. No small arteries (G≥7) were detected. Conclusions. For our preliminary masked analysis, two groups of venous trees with and without small veins (G≥7) were clearly revealed by VESGEN. Upon completing all images and unmasking the subject status of before and after HDT, we will determine whether differences in the presence or absence of small veins are important correlates, and perhaps reliable predictors, of other ocular and physiological adaptations to prolonged head-down tilt and microgravity. Clinical methods for examining adaptive microvascular remodeling in the retina to microgravity space flight are not currently established.

Fractal↗
NASA NTRS2016

Analysis by NASA's VESGEN Software of Vascular Branching in the Human Retina with a Ground-Based Microgravity Analog

Significant risks for visual impairment were discovered recently in astronauts following spaceflight, especially after long-duration missions.1 We hypothesize that microgravity-induced fluid shifts result in pathological changes within the retinal vasculature that precede visual and other ocular impairments. We therefore are analyzing retinal vessels in healthy subjects with NASA's VESsel GENeration Analysis (VESGEN) software2 before and after head-down tilt (HDT), a ground-based microgravity analog For our preliminary study of masked images, two groups of venous trees with and without small veins (G≥7) were clearly identified by VESGEN analysis. Upon completing all images and unmasking the subject status of pre- and post- HDT, we will determine whether differences in the presence or absence of small veins are important correlates, and perhaps reliable predictors, of other ocular and physiological adaptations to prolonged HDT and microgravity. Greater peripapillary retinal thickening was measured following 70-day HDT bed rest than 14-day HDT bed rest, suggesting that time of HDT may increase the amount of optic disc swelling.3 Spectralis OCT detected retinal nerve fiber layer thickening post HDT, without clinical signs of optic disc edema. Such changes may have resulted from HDT-induced cephalad fluid shifts. Clinical methods for examining adaptive microvascular remodeling in the retina to microgravity space flight are currently not established.

Retina↗