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The Potential for Collocated AGLP and ERBE Data for Fire, Smoke, and Radiation Budget Studies

One month of the Advanced Very High Resolution Radiometer (AVHRR) Global Area Coverage (GAC) Land Pathfinder (AGLP) data from September 1985 are used to examine the spatial and temporal distribution of fires over four major ecosystems in South America. The Earth Radiation Budget Experiment (ERBE) scanner data are used to examine the top of atmosphere (TOA) shortwave and longwave fluxes over smoke generated from biomass burning. The relationship between the AGLP-derived Normalized Difference Vegetation Index (NDVI) and the ERBE-estimated clear sky albedos are also examined as a function of the four ecosystems. This study shows that the grassland areas in South America have the highest number of tires for September 1985, and their corresponding NDVI values are smaller than the tropical rainforest region where the number of fires were comparatively small. Clear sky statistics accumulated during the days when smoke was not present show that clear sky albedos derived from ERBE are higher for grassland areas when compared to the tropical rainforest. The results show that the AGLP can be used to determine the spatial and temporal distribution of fires along with vegetation characteristics, while ERBE data can provide necessary information on broadband albedos and regional top of atmosphere radiative impacts of biomass burning aerosols. Since the AGLP data are available from 1981 to the present day, several climate-related issues can be addressed.

Christopher, S. A.↗

Widespread Increase of Boreal Summer Dry Season Length Over the Congo Rainforest

Dry season length strongly influences tropical rainforest vegetation and is largely determined by precipitation patterns. Over the Amazon, the dry season length has increased since 1979 and severe short-term droughts have occurred. However, similar changes have not been investigated for the world’s second largest rainforest, the Congo Basin, where long-term drying and large-scale declines in forest greenness and canopy water content were reported. Here we present observational evidence for widespread increases in the boreal summer (June–August) dry season length over the Congo Basin since the 1980s, from both hydrological and ecological perspectives. We analyzed both dry season onset and dry season end via multiple independent precipitation and satellite derived vegetation datasets for the period 1979–2015. The dry season length increased by 6.4–10.4 days per decade in the period 1988–2013, primarily attributed to an earlier dry season onset and a delayed dry season end. The earlier dry season onset was caused by long-term droughts due to decreased rainfall in the pre-dry season (April–June). The delayed dry season end resulted from insufficiently replenished soil moisture, which postpones the start of the next wet season and hinders vegetation regrowth. If such changes continue, the enhanced water stress in a warming climate may affect the carbon cycle and alter the composition and structure of evergreen rainforest.

Congo rainforest growing season NDVI SIF↗

Understanding Climate-Vegetation Interactions in Global Rainforests Through a GP-Tree Analysis

The tropical rainforests are the largest reserves of terrestrial carbon and, therefore, the future of these rainforests is a question that is of immense importance in the geoscience research community. With the recent severe Amazonian droughts in 2005 and 2010 and on-going drought in the Congo region for more than two decades, there is growing concern that these forests could succumb to precipitation reduction, causing extensive carbon release and feedback to the carbon cycle. However, there is no single ecosystem model that quantifies the relationship between vegetation health in these rainforests and climatic factors. Small scale studies have used statistical correlation measure and simple linear regression to model climate-vegetation interactions, but suffer from the lack of comprehensive data representation as well as simplistic assumptions about dependency of the target on the covariates. In this paper we use genetic programming (GP) based symbolic regression for discovering equations that govern the vegetation climate dynamics in the rainforests. Expecting micro-regions within the rainforests to have unique characteristics compared to the overall general characteristics, we use a modified regression-tree based hierarchical partitioning of the space to build individual models for each partition. The discovery of these equations reveal very interesting characteristics about the Amazon and the Congo rainforests. Our method GP-tree shows that the rainforests exhibit tremendous resiliency in the face of extreme climatic events by adapting to changing conditions.

genetic programming↗

Metagenomic Characterization of Soil Microbial Communities in the Luquillo Experimental Forest (Puerto Rico) and Implications for Nitrogen Cycling

The phylogenetic and functional diversities of microbial communities in tropical rainforests and how these differ from those of temperate communities remain poorly described but are directly related to the increased fluxes of greenhouse gases such as nitrous oxide (N 2 O) from the tropics. Toward closing these knowledge gaps, in this study we analyzed replicated shotgun metagenomes representing distinct life zones and an elevation gradient from four locations in the Luquillo Experimental Forest (LEF), Puerto Rico. These soils had a distinct microbial community composition and lower species diversity compared to those of temperate grasslands or agricultural soils. In contrast to the overall distinct community composition, the relative abundances and nucleotide sequences of N 2 O reductases (nosZ) were highly similar between tropical forest and temperate soils. However, respiratory NO reductase (norB) was 2-fold more abundant in the tropical soils, which might be relatable to their greater N 2 O emissions. Nitrogen fixation (nifH) also showed higher relative abundance in rainforest than in temperate soils, i.e., 20% versus 0.1 to 0.3% of bacterial genomes in each soil type harbored the gene, respectively. Finally, unlike temperate soils, LEF soils showed little stratification with depth in the first 0 to 30 cm, with ~45% of community composition differences explained solely by location. Collectively, these results advance our understanding of spatial diversity and metabolic repertoire of tropical rainforest soil communities and should facilitate future ecological studies of these ecosystems.

54 ENVIRONMENTAL SCIENCES↗

Fungal mat growth and leaf colonization at the TRACE warming experiment, Mar - Aug 2024, Luquillo, Puerto Rico

This data package contains processed measurements on the growth of litter mat-forming fungi and the time to leaf colonization at the Tropical Responses to Altered Climate Experiment (TRACE). Located near the Sabana Field Research Station in Luquillo, Puerto Rico, the TRACE site is located in a mature, closed-canopy tropical rainforest within the Luquillo Experimental Forest (LEF). These data quantify fungal mat growth and the time to leaf colonization of fungi species Gymnopus johnstonii and Marasmius crinis-equi. The experiment was conducted in ambient (control) and experimentally warmed plots (4°C above ambient) during spring and summer periods to assess how litter mat-forming fungi respond to a range of environmental conditions of tropical wet forests. The data files include tables of relative fungal mat growth rates, time to leaf colonization, averages of soil temperature (°C), and number of dry days before leaf attachment. The data are stored in comma-separated values (CSV) format and viewable with any text editor, spreadsheet, or statistical software (e.g., R, Python, Excel). Associated metadata describe plot identifiers, measurement descriptions, and processing steps.

Agaric fungi↗

Elucidating Drought-Tolerance Mechanisms in Plant Roots through 1H NMR Metabolomics in Parallel with MALDI-MS, and NanoSIMS Imaging Techniques

As direct mediators between plants and soil, roots play an important role in metabolic responses to environmental stresses such as drought, yet these responses are vastly uncharacterized on a plant-specific level, especially for co-occurring species. Here, we aim to examine the effects of drought on root metabolic profiles and carbon allocation pathways of three tropical rainforest species by combining cutting-edge metabolomic and imaging technologies in an in situ position-specific 13C-pyruvate root-labeling experiment. Further, washed (rhizosphere-depleted) and unwashed roots were examined to test the impact of microbial presence on root metabolic pathways. Drought had a species-specific impact on the metabolic profiles and spatial distribution in Piper sp. and Hibiscus rosa sinensis roots, signifying different defense mechanisms; Piper sp. enhanced root structural defense via recalcitrant compounds including lignin, while H. rosa sinensis enhanced biochemical defense via secretion of antioxidants and fatty acids. In contrast, Clitoria fairchildiana, a legume tree, was not influenced as much by drought but rather by rhizosphere presence where carbohydrate storage was enhanced, indicating a close association with symbiotic microbes. This study demonstrates how multiple techniques can be combined to identify how plants cope with drought through different drought-tolerance strategies and the consequences of such changes on below-ground organic matter composition.

Drought, Carbon cycle, MALDI-MSI, Metabolomics, Na↗

Evidence of an Intrinsic Intraseasonal Oscillation over Tropical South America During Austral Summer

The intraseasonal variation (ISV) in the 30-60 day band, also known as Madden-Julian oscillation (MJO), has been studied for decades. Madden and Julian showed that the oscillation originated from the western Indian Ocean, propagated eastward, got enhanced over the maritime continent and weakened after passing over the dateline. Composite studies showed evidences of a signal in upper and lower level zonal wind propagating around the globe during an oscillation. Theoretical studies pointed out that the interaction with the warm ocean surface and the coupling with the convective and radiative processes in the atmosphere could manifest the oscillation, which propagates eastward via mutual feedbacks between the wave motions and the cumulus heating. Over tropical South America, no independent 30-60 day oscillation has been reported so far, despite that Amazon is the most distinct tropical convection center over the western hemisphere and the fluxes from its surface of tropical rainforests are close to that from the warm tropical ocean. Liebmann et al. showed a distinct spectral peak of 40-50 day oscillation in outgoing longwave radiation (OLR) over tropical South America and considered that was manifested by the MJO propagation. Nogues-Paegle et al. (2000) focused on a dipole pattern of the OLR anomaly with centers of action over the South Atlantic Convergence Zone (SACZ) and the subtropical plain. They used the regional 10-90 day filtered data and demonstrated this pattern could be represented by the fifth mode of the rotated empirical orthogonal function. Its principal component was further analyzed using the singular spectrum analysis. Their result showed two oscillatory modes with periods of 36-40 days and 22-28 days, of which the former was related to the MJO influence and the latter linked to the remote forcing over southwest of Australia, which produced a wave train propagating southeastward, rounding the southern tip of South America and returning back toward the northeast. The 22-28 day mode has distinct impact on SACZ, responsible for the regional seesaw pattern of alternating dry and wet conditions. In this study we will focus on the 30-60-day spectral band and investigate whether the independent oscillation source over tropical South America is existed. First, we will show the seasonal dependence of the tropical South American ISV in Section 3. Then, the leading principal modes of 30-60 day bandpass filtered 850-hPa velocity potential (VP850) will be computed to distinguish the stationary ISV over tropical South America (SISA) from the propagating MJO in the austral summertime in Section 4. The importance of SISA in representing the regional ISV over South America will be discussed. In Section 5, we will demonstrate the mass oscillation regime of SISA, which is well separated from that of MJO by the Andes, and the convective coupling with rainfall. The dynamical response of SISA and the impact on the South American summer monsoon (SASM) will be presented. Finally, we will give the concluding remarks.

Zhou, Jiayu↗

Mapping Deforestation and Land Use in Amazon Rainforest Using SAR-C Imagery

Land use changes and deforestation in tropical rainforests are among the major factors affecting the overall function of the global environment. To routinely assess the spatial extend and temporal dynamics of these changes has become an important challenge in several scientific disciplines such as climate and environmental studies. In this paper, the feasibility of using polarimetric spaceborne SAR data in mapping land cover types in the Amazon is studied.

SAR-C Imagery Amazon Rainforest Land Use Deforesta↗

Terrestrial photosynthesis inferred from plant carbonyl sulfide uptake

Terrestrial photosynthesis, or gross primary production (GPP), is the largest carbon flux in the biosphere, but its global magnitude and spatiotemporal dynamics remain uncertain. The global annual mean GPP is historically thought to be around 120 PgC yr −1 , which is about 30–50 PgC yr −1 lower than GPP inferred from the oxygen-18 ( 18 O) isotope and soil respiration. This disparity is a source of uncertainty in predicting climate–carbon cycle feedbacks. Here, in this study, we infer GPP from carbonyl sulfide, an innovative tracer for CO 2 diffusion from ambient air to leaf chloroplasts through stomata and mesophyll layers. We demonstrate that explicitly representing mesophyll diffusion is important for accurately quantifying the spatiotemporal dynamics of carbonyl sulfide uptake by plants. From the estimate of carbonyl sulfide uptake by plants, we infer a global contemporary GPP of 157 (±8.5) PgC yr −1 , which is consistent with estimates from 18 O (150–175 PgC yr −1 ) and soil respiration ($149^{+29}_{-23}$) PgC yr −1 ), but with an improved confidence level. Our global GPP is higher than satellite optical observation-driven estimates (120–140 PgC yr –1 ) that are used for Earth system model benchmarking. This difference predominantly occurs in the pan-tropical rainforests and is corroborated by ground measurements, suggesting a more productive tropics than satellite-based GPP products indicated. As GPP is a primary determinant of terrestrial carbon sinks and may shape climate trajectories, our findings lay a physiological foundation on which the understanding and prediction of carbon–climate feedbacks can be advanced.

Lai, Jiameng↗

Seasonal and long-term variations in leaf area of Congolese rainforest

It is important to understand temporal and spatial variations in the structure and photosynthetic capacity of tropical rainforests in a world of changing climate, increased disturbances and human appropriation. The equatorial rainforests of Central Africa are the second largest and least disturbed of the biodiversly-rich and highly productive rainforests on Earth. Currently, there is a dearth of knowledge about the phenological behavior and long-term changes that these forests are experiencing. Here, this study reports on leaf area seasonality and its time trend over the past two decades as assessed from multiple remotely sensed datasets. Seasonal variations of leaf area in Congolese forests derived from MODIS data co-vary with the bimodal precipitation pattern in this region, with higher values during the wet season. Independent observational evidence derived from MISR and EPIC sensors in the form of angular reflectance signatures further corroborate this seasonal behavior of leaf area. The bimodal patterns vary latitudinally within this large region. Two sub-seasonal cycles, each consisting of a dry and wet season, could be discerned clearly. These exhibit different sensitivities to changes in precipitation. Contrary to a previous published report, no widespread decline in leaf area was detected across the entire extent of the Congolese rainforests over the past two decades with the latest MODIS Collection 6 dataset. Long-term precipitation decline did occur in some localized areas, but these had minimal impacts on leaf area, as inferred from MODIS and MISR multi-angle observations.

54 ENVIRONMENTAL SCIENCES↗

Techno-Economic Analysis of Geologically Connected Seawater Air Conditioning (GeoSWAC) Concept for District Cooling at the University of Puerto Rico at Rio Piedras

At the University of Puerto Rico at Rio Piedras, a central chilled water plant supplies cooling to several campus buildings, contributing significantly to electricity demand during daytime peak hours, particularly in the summer months. These operational challenges are exacerbated by Puerto Rico's tropical rainforest climate and a power grid vulnerable to frequent disruptions caused by hurricanes and tropical storms. This study presents a techno-economic analysis of the existing chilled water plant serving four representative campus buildings and introduces a conceptual alternative: the Geologically connected Seawater Air Conditioning (GeoSWAC) system. GeoSWAC leverages stable low temperatures of deep ocean water (~1 km depth), hydraulically connected to an inland well, to deliver cooling without the use of vapor-compression refrigeration. Using modeled annual cooling loads and chiller performance data, capital costs, energy consumption, and levelized cost of cooling (LCOC) were evaluated for both systems. While GeoSWAC showed higher capital costs than the chiller-based scenario, operational costs were significantly lower at $26k-$53k annually, resulting in a lower LCOC between $2.3/MWh and $8.2/MWh compared to $30.2/MWh-$33.6/MWh for the chiller scenario. These results suggest that the GeoSWAC system offers a promising, low-energy, and climate-resilient alternative for large-scale cooling in tropical coastal environments, with significant potential to reduce peak electricity demand and improve long-term system reliability.

15 GEOTHERMAL ENERGY↗

Techno-Economic Analysis of Geologically Connected Seawater Air Conditioning (GeoSWAC) Concept for District Cooling at the University of Puerto Rico at Rio Piedras: Preprint

At the University of Puerto Rico at Rio Piedras, a central chilled water plant supplies cooling to several campus buildings, contributing significantly to electricity demand during daytime peak hours, particularly in the summer months. These operational challenges are exacerbated by Puerto Rico's tropical rainforest climate and a power grid vulnerable to frequent disruptions caused by hurricanes and tropical storms. This study presents a techno-economic analysis of the existing chilled water plant serving four representative campus buildings and introduces a conceptual alternative: the Geologically connected Seawater Air Conditioning (GeoSWAC) system. GeoSWAC leverages stable low temperatures of deep ocean water (~1 km depth), hydraulically connected to an inland well, to deliver cooling without the use of vapor-compression refrigeration. Using modeled annual cooling loads and chiller performance data, capital costs, energy consumption, and levelized cost of cooling (LCOC) were evaluated for both systems. While GeoSWAC showed higher capital costs than the chiller-based scenario, operational costs were significantly lower at $26k-$53k annually, resulting in a lower LCOC between $2.3/MWh and $8.2/MWh compared to $30.2/MWh-$33.6/MWh for the chiller scenario. These results suggest that the GeoSWAC system offers a promising, low-energy, and climate-resilient alternative for large-scale cooling in tropical coastal environments, with significant potential to reduce peak electricity demand and improve long-term system reliability.

15 GEOTHERMAL ENERGY↗

Quantifying Uncertainties in Land-Surface Microwave Emissivity Retrievals

Uncertainties in the retrievals of microwaveland-surface emissivities are quantified over two types of land surfaces: desert and tropical rainforest. Retrievals from satellite-based microwave imagers, including the Special Sensor Microwave Imager, the Tropical Rainfall Measuring Mission Microwave Imager, and the Advanced Microwave Scanning Radiometer for Earth Observing System, are studied. Our results show that there are considerable differences between the retrievals from different sensors and from different groups over these two land-surface types. In addition, the mean emissivity values show different spectral behavior across the frequencies. With the true emissivity assumed largely constant over both of the two sites throughout the study period, the differences are largely attributed to the systematic and random errors inthe retrievals. Generally, these retrievals tend to agree better at lower frequencies than at higher ones, with systematic differences ranging 1%-4% (3-12 K) over desert and 1%-7% (3-20 K) over rainforest. The random errors within each retrieval dataset are in the range of 0.5%-2% (2-6 K). In particular, at 85.5/89.0 GHz, there are very large differences between the different retrieval datasets, and within each retrieval dataset itself. Further investigation reveals that these differences are most likely caused by rain/cloud contamination, which can lead to random errors up to 10-17 K under the most severe conditions.

Land-Surface↗

Approaches to vegetation mapping and ecophysiological hypothesis testing using combined information from TIMS, AVIRIS, and AIRSAR

The Tropical Rainforest Ecology Experiment (TREE) had two primary objectives: (1) to design a method for mapping vegetation in tropical regions using remote sensing and determine whether the result improves on available vegetation maps; and (2) to test a specific hypothesis on plant/water relations. Both objectives were thought achievable with the combined information from the Thermal Infrared Multispectral Scanner (TIMS), Airborne Visible/Infrared Imaging Spectrometer (AVIRIS), and Airborne Synthetic Aperture Radar (AIRSAR). Implicitly, two additional objectives were: (1) to ascertain that the range within each variable potentially measurable with the three instruments is large enough in the site, relative to the sensitivity of the instruments, so that differences between ecological groups may be detectable; and (2) to determine the ability of the three systems to quantify different variables and sensitivities. We found that the ranges in values of foliar nitrogen concentration, water availability, stand structure and species composition, and plant/water relations were large, even within the upland broadleaf vegetation type. The range was larger when other vegetation types were considered. Unfortunately, cloud cover and navigation errors compromised the utility of the TIMS and AVIRIS data. Nevertheless, the AIRSAR data alone appear to have improved on the available vegetation map for the study area. An example from an area converted to a farm is given to demonstrate how the combined information from AIRSAR, TIMS, and AVIRIS can uniquely identify distinct classes of land use. The example alludes to the potential utility of the three instruments for identifying vegetation at an ecological scale finer than vegetation types.

Oren, R.↗

The Amazon and climate

The climatologies of cloudiness and precipitation for the Amazon, are reviewed and the physical causes of some of the observed features and those which are not well known are explained. The atmospheric circulation over the Amazon is discussed on the large scale tropical circulations forced by deep diabatic heating sources. Weather deforestation which leads to a reduction in evapotranspiration into the atmosphere, and a reduction in precipitation and its implicated for the gobal climate is discussed. It is indicated that a large scale clearing of tropical rainforests there would be a reduction in rainfall which would have global effects on climate and weather both in the tropical and extratropical regions.

Nobre, C. A.↗

Regional Atmospheric CO2 Inversion Reveals Seasonal and Geographic Differences in Amazon Net Biome Exchange

Understanding tropical rainforest carbon exchange and its response to heat and drought is critical for quantifying the effects of climate change on tropical ecosystems, including global climate carbon feedbacks. Of particular importance for the global carbon budget is net biome exchange of CO2 with the atmosphere (NBE), which represents nonfire carbon fluxes into and out of biomass and soils. Subannual and sub-Basin Amazon NBE estimates have relied heavily on process-based biosphere models, despite lack of model agreement with plot-scale observations. We present a new analysis of airborne measurements that reveals monthly, regional-scale (Approx.1-8 x 10(exp -6) km2) NBE variations. We develop a regional atmospheric CO2 inversion that provides the first analysis of geographic and temporal variability in Amazon biosphere-atmosphere carbon exchange and that is minimally influenced by biosphere model-based first guesses of seasonal and annual mean fluxes. We find little evidence for a clear seasonal cycle in Amazon NBE but do find NBE sensitivity to aberrations from long-term mean climate. In particular, we observe increased NBE (more carbon emitted to the atmosphere) associated with heat and drought in 2010, and correlations between wet season NBE and precipitation (negative correlation) and temperature (positive correlation). In the eastern Amazon, pulses of increased NBE persisted through 2011, suggesting legacy effects of 2010 heat and drought. We also identify regional differences in postdrought NBE that appear related to long-term water availability. We examine satellite proxies and find evidence for higher gross primary productivity (GPP) during a pulse of increased carbon uptake in 2011, and lower GPP during a period of increased NBE in the 2010 dry season drought, but links between GPP and NBE changes are not conclusive. These results provide novel evidence of NBE sensitivity to short-term temperature and moisture extremes in the Amazon, where monthly and sub-Basin estimates have not been previously available.

precipitation↗

Rainforest-to-pasture conversion stimulates soil methanogenesis across the Brazilian Amazon

The Amazon rainforest is a biodiversity hotspot and large terrestrial carbon sink threatened by agricultural conversion. Rainforest-to-pasture conversion stimulates the release of methane, a potent greenhouse gas. The biotic methane cycle is driven by microorganisms; therefore, this study focused on active methane-cycling microorganisms and their functions across land-use types. We collected intact soil cores from three land use types (primary rainforest, pasture, and secondary rainforest) of two geographically distinct areas of the Brazilian Amazon (Santarém, Pará and Ariquemes, Rondônia) and performed DNA stable-isotope probing coupled with metagenomics to identify the active methanotrophs and methanogens. At both locations, we observed a significant change in the composition of the isotope-labeled methane-cycling microbial community across land use types, specifically an increase in the abundance and diversity of active methanogens in pastures. We conclude that a significant increase in the abundance and activity of methanogens in pasture soils could drive increased soil methane emissions. Furthermore, we found that secondary rainforests had decreased methanogenic activity similar to primary rainforests, and thus a potential to recover as methane sinks, making it conceivable for forest restoration to offset greenhouse gas emissions in the tropics. These findings are critical for informing land management practices and global tropical rainforest conservation.

59 BASIC BIOLOGICAL SCIENCES↗

Microbial community data from throughfall exclusion experiment: Metadata, SI, community composition, LefSe, and FunGuilR data tables from PARCHED Panama tropical forest soils, 2024-2025

Soil contains more carbon (C) than terrestrial vegetation and the atmosphere combined, with some of the largest terrestrial C stocks in tropical rainforests. Soil microbes decompose organic matter, playing a vital role in the storage or loss of soil C. With climate change, drought conditions are predicted to increase in many tropical regions, including both chronic drying and extended drought, potentially influencing these processes. This project explored the effects of chronic and seasonal drying on soil microbial communities across four distinct tropical forests in a long-term drying experiment. We investigated the effects of a chronic drying manipulation on soil microbial community abundance and variation across different forests and seasons. We also compared findings with previously published data from these forests after short-term drying. This project used soils from a long-term drying experiment established in 2018 across four seasonal lowland forests in Panama. Soils were collected from 0 – 10 cm depths during three seasonal periods in control and drying plots in 2024 and 2025 from a total of 32 plots (n = 4 per forest per treatment). The forests varied in baseline rainfall and soil fertility. We calculated alpha and beta diversity indices and compared taxonomic community composition. We found significant biogeographic variation in microbial diversity and taxonomy, with significant differences across the forests and significant effects of the drying treatment. Metadata and sample IDs are within Metadata_16S.csv and Metadata_ITS.csv. Relative abundance tables of every sample at every season are shown in the Excel workbooks 16S Relative Abundance.xlsx and ITS Relative Abundance.xlsx. They are then also shown in CSV files by each taxonomic level. Linear discriminant analysis effect size (LefSe) tables are shown for the full 16S and ITS datasets (n = 96), subsets for every site at every season (n = 8), and then for the forests with each plot merged by season (n = 8). FunGuildR data table of ITS data is uploaded.

Bacteria↗