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Skeletal muscle fiber, nerve, and blood vessel breakdown in space-flown rats

Histochemical and ultrastructural analyses were performed postflight on hind limb skeletal muscles of rats orbited for 12.5 days aboard the unmanned Cosmos 1887 biosatellite and returned to Earth 2 days before sacrifice. The antigravity adductor longus (AL), soleus, and plantaris muscles atrophied more than the non-weight-bearing extensor digitorum longus, and slow muscle fibers were more atrophic than fast fibers. Muscle fiber segmental necrosis occurred selectively in the AL and soleus muscles; primarily, macrophages and neutrophils infiltrated and phagocytosed cellular debris. Granule-rich mast cells were diminished in flight AL muscles compared with controls, indicating the mast cell secretion contributed to interstitial tissue edema. Increased ubiquitination of disrupted myofibrils implicated ubiquitin in myofilament degradation. Mitochondrial content and succinic dehydrogenase activity were normal, except for subsarcolemmal decreases. Myofibrillar ATPase activity of flight AL muscle fibers shifted toward the fast type. Absence of capillaries and extravasation of red blood cells indicated failed microcirculation. Muscle fiber regeneration from activated satellite cells was detected. About 17% of the flight AL end plates exhibited total or partial denervation. Thus, skeletal muscle weakness associated with spaceflight can result from muscle fiber atrophy and segmental necrosis, partial motor denervation, and disruption of the microcirculation.

NASA Experiment Number COS 1887-6↗

Premixed Atmosphere and Convection Influences on Flame Inhibition and Combustion (Pacific)

Flame spread over flat solid fuel beds is a useful paradigm for studying the behavior of more complex two-phase nonpremixed flames. For practical applications, two of the most important elements of flame spreading are the effects of (1) the ambient atmosphere (e.g. pressure and composition) and (2) the flow environment on the spread rate and extinction conditions. Concerning (1), studies of flame spread in vitiated air and non-standard atmospheres such as those found in undersea vessels and spacecraft are particularly important for the assessment of fire hazards in these environments as well as determination of the effectiveness of fire suppressants. Concerning (2), the flow environment may vary widely even when no forced flow is present because of buoyancy effects. Consequently, the goal of this work is to employ microgravity (micro g) experiments to extend previous studies of the effects of ambient atmosphere and the flow environment on flame spread through the use of microgravity (micro g) experiments. Because of the considerable differences between upward (concurrent-flow) and downward (opposed-flow) flame spread at 1g (Williams, 1976, Fernandez-Pello, 1984), in this work both upward and downward 1g spread are tested. Two types of changes to the oxidizing atmosphere are considered in this work. One is the addition of sub-flammability-limit concentrations of a gaseous fuel ('partially premixed' atmospheres). This is of interest because in fires in enclosures, combustion may occur under poorly ventilated conditions, so that oxygen is partially depleted from the air and is replaced by combustible gases such as fuel vapors, H2 or CO. Subsequent fire spread over the solid fuel could occur under conditions of varying oxygen and gaseous fuel content. The potential significance of flame spread under vitiated or partially premixed conditions has been noted previously (Beyler, 1984). The second change is the diluent type, which affects the radiative properties of the mixture as well as the Lewis number (Le) of the reactants in the atmosphere, which for oxygen is defined as the thermal diffusivity of the bulk mixture divided by the mass diffusivity of oxygen into the bulk mixture. Understanding the effect of diluent type is desirable because in some undersea and spaceborne habitations, it is desirable to use diluent gases other than nitrogen. Prior experiments have shown that both radiation (Bhattacharjee and Altenkirch, 1993) and Lewis number (Zhang et al, 1992) effects are important in flame spreading problems.

Honda, Linton K.↗

Assessment of Nutrient Stability in Space Foods

Maintaining an intact nutrient supply in the food system flown on spacecraft is a critical issue for mission success and crew health and safety. Early polar expeditions and exploration expeditions by sailing vessels have taught us that a deficiency, or excess, of even a single vitamin in the food supply can be catastrophic. Evidence from ground-based research indicates that some vitamins are destroyed and fatty acids are oxidized (and therefore rendered dangerous or useless) by different types of radiation and by conditions of long-term storage. We hypothesize that radiation and long-term storage in the space-flight environment will affect the stability of vitamins, amino acids, and fatty acids in the space food system. The research objectives of our ongoing stability studies are to determine the stability of water- and fat-soluble vitamins, fatty acids, and amino acids in the space food supply before and after space flight on the International Space Station (ISS). Foods were analyzed after 2 weeks (a flight control), 11, 19, and 28 months of flight. Along with the space-flown foods, ground-based controls matched for time, light, and temperature are analyzed. The flight studies complement planned ground-based studies of the effects of radiation on vitamins, amino acids, and fatty acids. Flight studies are needed because a model based on ground-based data cannot predict all of the effects of the space-flight environment. Flight studies provide a more accurate test system to determine the effects on these nutrients of the temperature, and radiation conditions in the space-flight environment. Ground studies are required to evaluate longer missions and higher radiation levels expected outside low-Earth orbit. In addition to providing information about nutrient stability in space, the results of these studies will help NASA determine if a need exists to develop special packaging that can ensure stability of foods and nutrients in space, or if further studies of nutrient metabolism or nutrient requirements are needed.

Zwart, S. R.↗

A Fundamental Study of Nucleate Pool Boiling Under Microgravity

An experimental study of incipient boiling in short-term microgravity and with a/g = +/- 1 for pool boiling was performed. Calibrated thin gold films sputtered on a smoothly polished quartz surface were used simultaneously for thermal-resistance measurements and heating of the boiling surface. The gold films were used for both transient and quasi-steady heating surface temperature measurements. Two test vessels were constructed for precise measurement and control of fluid temperature and pressure: a laboratory pool boiling vessel for the a/g = +/- 1 experiments and a pool boiling vessel designed for the 131 m free-fall in the NASA Lewis Research Center Microgravity Research Facility for the microgravity tests. Measurements included the heater surface temperature, the pressure near the heating surface, the bulk liquid temperatures. High speed photography (up to 1,000 frames per second) was used in the experiments. With high quality microgravity and the measured initial temperature of the quiescent test fluid, R113, the temperature distribution in the liquid at the moment of boiling inception resulting from an imposed step in heat flux is known with a certainty not possible previously. The types of boiling propagation across the large flat heating surface, some observed here for the first time, are categorized; the conditions necessary for their occurrence are described. Explosive boiling propagation with a striking pattern of small scale protuberances over the entire vapor mass periphery not observed previously at low heat flux levels (on the order of 5 W/cm(exp 2)) is described. For the heater surface with a/g = -1, a step in the heater surface temperature of short duration was imposed. The resulting liquid temperature distribution at the moment of boiling inception was different from that obtained with a step in heat flux.

Ervin, Jamie S.↗

Solutal Convection Around Growing Protein Crystal and Diffusional Purification in Space

This work theoretically addressed two subjects: 1) onset of convection, 2) distribution of impurities. Onset of convection was considered analytically and numerically. Crystal growth was characterized by slow surface incorporation kinetics, i.e. growth kinetic coefficient beta (cm/s) small as compared to the typical bulk diffusion rate, D(sub 1)/h, where D(sub 1) is diffusivity of major crystallizing protein and h is the crystal size. Scaling type analysis predicted two laws on how the convection rate, v, essentially the Peclet number, Pe exactly equal to vh/D(sub 1), depends on dimensionless kinetic coefficient a exactly equal to beta h/D(sub 1). Namely: Pe = C(sub 2/5)(aRa(sup 2/5)) and Pe = C(sub 1) aRa. Here, Reynolds number Ra = rho(sub 1)(sup 0)gh(sup 3)(rho(sub p) - rho(sub w))/rho(sup p)rho(sub 1)vD(sub 1), v being solution viscosity. The constants C(sub 2/5), exactly equal to 0.28 and C(sub 1) exactly equal to 10(exp -2) found from the full scale computer simulation for a cylindrical crystal inside big cylindrical vessel. The linear boundary conditions connecting protein and impurity concentration at the interface with the flux to/from the interface was applied. No-slip condition for Navier-Shocker equations was employed. With these conditions, flow and concentration distributions were calculated. Validity of the Pe(Ra) dependencies follows for wide range of parameters for which numerical calculations have been accomplished and presented by various points.

Chernov, A. A.↗

Three-Dimensional Engineered High Fidelity Normal Human Lung Tissue-Like Assemblies (TLA) as Targets for Human Respiratory Virus Infections

Unlike traditional two-dimensional (2D) cell cultures, three-dimensional (3D) tissue-like assemblies (TLA) (Goodwin et aI, 1992, 1993, 2000 and Nickerson et aI. , 2001,2002) offer high organ fidelity with the potential to emulate the infective dynamics of viruses and bacteria in vivo. Thus, utilizing NASA micro gravity Rotating Wall Vessel (RWV) technology, in vitro human broncho-epithelial (HBE) TLAs were engineered to mimic in vivo tissue for study of human respiratory viruses. These 3D HBE TLAs were propagated from a human broncho-tracheal cell line with a mesenchymal component (HBTC) as the foundation matrix and either an adult human broncho-epithelial cell (BEAS-2B) or human neonatal epithelial cell (16HBE140-) as the overlying element. Resulting TLAs share several characteristic features with in vivo human respiratory epithelium including tight junctions, desmosomes and cilia (SEM, TEM). The presence of epithelium and specific lung epithelium markers furthers the contention that these HBE cells differentiate into TLAs paralleling in vivo tissues. A time course of infection of these 3D HBE TLAs with human respiratory syncytial virus (hRSV) wild type A2 strain, indicates that virus replication and virus budding are supported and manifested by increasing virus titer and detection of membrane-bound F and G glycoproteins. Infected 3D HBE TLAs remain intact for up to 12 days compared to infected 2D cultures that are destroyed in 2-3 days. Infected cells show an increased vacuolation and cellular destruction (by transmission electron microscopy) by day 9; whereas, uninfected cells remain robust and morphologically intact. Therefore, the 3D HBE TLAs mimic aspects of human respiratory epithelium providing a unique opportunity to analyze, for the first time, simulated in vivo viral infection independent of host immune response.

Goodwin, T. J.↗

The Effect of Temperature on the Preservation of Volatile-Rich Lunar Samples

Introduction. The Moon’s south pole is a high-priority target for human exploration and scientific study. This interest is, in part, due to the presence of Permanently Shadowed Regions (PSRs), which could contain high concentrations of unique volatiles at cryogenic temperatures [1]. Returned samples from PSRs may include a unique combination of rocks, regolith, and volatile species, providing unprecedented insights into the history of the Solar System and the potential for resource utilization on the Moon. However, because PSR samples are cryogenic up-on collection, lunar polar sample return will eventually require cold stowage for the journey from the Moon to Earth. Without cold stowage, PSR sample return will likely result in phase changes and chemical reactions within the volatile component of the sample, which would negatively impact the resulting scientific studies of those samples. This abstract summarizes the initial results from an ongoing characterization of analog PSR samples at a range of temperatures, with the goal of defining the temperatures needed for a flight cold stowage freezer. Background. Based on remote sensing observations of the Moon [2], south polar PSRs range in temperature from ~120K for small and/or shallow PSRs to ~20K at the most extreme locations in large, deep PSRs. A range of volatiles have been hypothesized to exist at the surface or subsurface of the lunar poles [3-5 and others]. This hypothesis was verified when the LCROSS mission impacted the <50-K PSR in the crater Cabeus, detecting a range of volatiles from water to low condensation temperature species such as H2S and methane [6]. Species such as H2S and ammonia (also detected by LCROSS) are also highly reactive, and increase the likelihood of chemical reactions at elevated (non-cryogenic) temperatures. At the Johnson Space Center’s Planetary Exploration and Astromaterials Research Laboratory (JSC-PEARL), we have developed a volatile-bearing lunar simulant that incorporates several of the species detected by LCROSS [Table 1] mixed cryogenically with the USGS Lunar Highlands Type (LHT) regolith simulant. The new volatile-regolith simulant will be used to assess the degree of sample alteration at room temperature, -20°C, -80°C, and -196°C (liquid nitro-gen), over a two-week period. Room temperature samples represent those likely to be returned during initial missions without cold stowage, -20°C provides an analog to Apollo cold curated samples, -80°C is the temperature of multiple flight payload freezers (e.g., MELFI), and -196°C is analogous to lunar PSRs. Two weeks is an approximation of the time between sample collection and Earth return for initial Artemis missions. Over this period of time, sample head-space gases will be analyzed using a Universal Gas Analyzer (UGA, a type of mass spectrometer) coupled with a Baratron pressure sensor. After testing, the regolith component of the simulant will be purged of volatiles and preserved for future electron beam and/or FTIR analysis. Experimental Procedure. Volatile-regolith simulants will be produced as an initial homogenous batch; this batch will then be distributed into aliquots (gas chromatography/GC vials or cryo vials), ensuring that each sample has the same starting composition and conditions [Fig. 1]. In addition to the “full” simulant shown in Table 1, less complex simulant compositions will be used as baseline and control samples [Table 2]. Aliquots will be produced in triplicate for each simulant composition, storage temperature, and date of sampling. Headspace gases in all Day 0 samples will be analyzed by the UGA immediately. Cold storage samples for future analytical days will be placed in freezers appropriate to their target temperatures (-20°C, -80°C, -196°C). For ambient-temperature samples, regolith and regolith-water samples will be stored in a fume hood, while the full simulant will be stored in a sealed Parr vessel for safety; no other simulants (RWCM/ RWCM+) will be stored at ambient temperature for this test. Samples will be analyzed by UGA in this manner on each Analysis Day outlined in Table 2. Analytical Data. The UGA measures the partial pressures in a single sample aliquot over a set mass range of 0-105 atomic mass units (AMU) [Figure 2]; this set range was selected to slightly exceed the mass of the highest-mass expected reaction product (H2SO4). The Baratron complements the UGA by measuring the total pressure in the headspace of a sample vial. Coupled together, the quantitative abundances of gases will be monitored throughout the test. UGA analyses of the triplicate samples for each storage temperature, day, and simulant composition will be averaged, and standard deviations for each will be calculated. The compositions of starting species (shown in Table 1) will be characterized as a function of time, and the presence of any new compounds (reaction products) will be monitored as well. Total pressures will be recorded for each sample analysis, and any samples that show signs of leakage (e.g., a significant reduction in pressure or simulant volatiles) will be discarded. Anticipated Results. Testing is planned to begin in January 2022. The resulting data will allow compositional and phase changes in the volatile component of the simulants to be determined. Both the reduction in initial compounds and the addition of reaction products are expected to be observed. In addition, the relative efficacy of the different temperatures at pre-serving the initial composition of the simulants will be quantified. Finally, the regolith component of each sample will be argon-purged and stored in a controlled environment for future laboratory analysis. Compositional and morphological changes in the regolith are expected for samples above 0°C. This test will be repeated three times over the course of 2022. Understanding the effect of temperature on both the volatile and regolith components of analog lunar materials will allow requirements for a cold stowage freezer to be developed. The implementation of cold stowage for lunar polar missions will maximize the preservation of returned samples, enabling ground-breaking lunar and Solar System volatiles science for decades to come.

J L Mitchell↗

Loss of Signal Transduction and Inhibition of Lymphocyte Locomotion in a Ground-Based Model of Microgravity

Inflammatory adherence to, and locomotion through the interstitium is an important component of the immune response. Conditions such as true gravity (TG) and modeled microgravity (MMG) severely inhibit lymphocyte locomotion in vitro through gelled Type I collagen (Pellis et al., 1994, 1997). We used the rotating-wall vessel bioreactor (RWV) as a prototype for modeled microgravity. After observing that lymphocyte locomotion was severely affected in modeled microgravity, we found that polyclonal activation of lymphocytes before exposure to modeled microgravity reversed the locomotion inhibition. Phorbol myristate acetate (PMA) treatment of normal peripheral blood lymphocytes, after exposure to modeled microgravity, restored lymphocyte locomotion by 84%. Calcium ionophore had no effect on modeled microgravity-exposed lymphocytes. Therefore, the signal pathways involving calcium may not be affected by modeled microgravity. However, direct activation of Protein Kinase C (PKC) with PMA was effective in restoring locomotion in modeled microgravity almost comparable to normal levels in lymphocytes cultured in static T flasks. Thus, events either at the level of PKC or upstream are affected by modeled microgravity. Treatment of lymphocytes with mitomycin C prior to exposure to modeled microgravity, followed by PMA, restored locomotion to the same extent as nonmitomycin C-treated lymphocytes exposed to modeled microgravity (80-85%). Therefore 1) new DNA synthesis is not necessary to restore locomotion and 2) traditional activation and locomotion share common pathways up to PKC. Thereafter the signals diverge. Furthermore PMA added immediately before or after initiation of modeled microgravity prevents the loss of lymphocyte locomotion.

Sundaresan, Alamelu↗

Acoustic Emission Health Monitoring of Fill Purge COPV's Used in Aerospace and Automotive Applications and Designed for Long Cycle Life

Cumulative composite damage in composite pressure vessels (CPVs) currently is not monitored on-orbit. Consequently, hazards due to catastrophic burst before leak (BBL) or compromised CPV reliability cannot be ascertained or mitigated, posing a risk to crew and mission assurance. The energy associated with CPV rupture can be significant, especially with high pressure gases are under containment, and the energy releases can be severe enough to cause injury, death, loss of assets or mission. Dual-Use Rationale: CPVs similar to those used by NASA on ISS, for example, are finding increasing use in automotive and transportation industry applications. These CPVs generally have a nonload sharing liner and are repeatedly filled over their service lifetime, typically with hydrogen or compressed natural gas (CNG). The same structural health monitoring equipment and software developed by NASA WSTF for evaluating, in real-time, the health of NASA CPVs on ISS will be used to evaluate the health of automotive CPVs, the only differences being the type and design of the CPV, and the in-service lifetime pressure histories. HSF Need(s)/Performance Characteristic(s) Supported: 1) Enable on-board vehicle systems management for mission critical functions at destinations with > 3 second time delay 2) Enable autonomous nominal operations and FDIR for crewed and un-crewed systems 3) Reduce on-board crew time to sustain and manage vehicle by factor of 2x at destinations with > 6 second time delay (see Crew Autonomy sheet) 4) Reduce earth-based mission ops "back room engineering" requirements for distant mission support delay (see Mission Autonomy sheet)

Waller, Jess↗

Influence of High Aspect Ratio Vessel Cell Culture on TNF-Alpha, Insulin Secretion and Glucose Homeostasis in Pancreatic Islets of Langerhans from Wistar Furth Rats

The present studies were carried out to determine the influence of a ground based microgravity paradigm, utilizing the High Aspect Ratio Vessel (HARV) cell culture upon lipopolysaccharide (LPS) stimulated tumor necrosis factor alpha (TNF-alpha) production of pancreatic islets of Langerhans. An additional aim was to elucidate alterations in insulin secretion and glucose utilization using the HARV low shear, gravity averaged vector, cell culture technique. Islets were isolated (1726 +/- 117, 150 micron islet equivalent units) from Wistar Furth rats and assigned to four treatment groups: 1) HARV, 2) HARV plus LPS, 3) static culture, 4) static culture plus LPS. Following 48 hours of culture, insulin concentration was increased in both HARV and static cultures (p<0.05). Islet medium from HARV and static cultures were assayed for TNF-alpha (L929 cytotoxicity assay) and was measured at selected time points for 48 hours. TNF-alpha was significantly increased in LPS-induced HARV and static cultures, yet the increase was more pronounced in the static culture group (p<0.05). This is a novel observation and indicates that TNF producing cells are present in islets and that LPS stimulates TNF secretion in isolated islets. A decrease in insulin concentration was demonstrated in the islet medium of the LPS stimulated HARV culture (p<0.05). That TNF-alpha is associated with a decreased insulin secretion is intriguing, both as it relates to in-flight investigations, and as it may provide insight into the pathophysiology of Type I and Type 11 diabetes. Glucose concentration in islet medium was lesser throughout the experiment in static cultures, suggesting a decreased reliance upon glucose as a metabolic substrate in the islets cultured in HARVS. In conclusion, the present studies demonstrate alterations in LPS induced TNF-alpha production of pancreatic islets of Langerhans, favoring a lesser TNF production in the microgravity HARV paradigm. Additionally, alterations in fuel homeostasis may be promulgated by HARV culture. The clinical and physiological significance of these observations remains to be determined.

Tobin, Brian W.a↗

SHADOZ (Southern Hemisphere ADditional OZonesondes): An Ozonesonde Network for Satellite Validation, Climatology and Modeling

In the past 5 years, new tropical ozone data products have been developed from TOMS and other satellites, During this period, global chemical-transport models have been used for ozone assessment studies. However, there has been a lack of independent ozone profiles in the tropics for evaluation of the data sets and models. In 1998, NASA's Goddard Space Flight Center, Wallops Flight Facility and NOAA's CMDL (Climate Monitoring and Diagnostics Lab), began a 2-year project to collect a consistent data set by augmenting ozonesonde launches at southern hemisphere tropical sites The measurements are available to the scientific community at a single electronic location - the SHADOZ website at NASA/Goddard: http://code9l6.gsfc.nasa.gov/Data services/Shadoz/shadoz hmpg2.html. Stations in SHADOZ include four islands in the Pacific: Fiji, Tahiti, San Cristobal (Galapagos) and American Samoa. Two sites are at and in the Atlantic: Natal (Brazil) and Ascension Island. Three other sites span Africa (Nairobi and Irene, South Africa) and the Indian Ocean (Reunion Island and Watukosek in Java, Indonesia). All SHADOZ sites are using ECC-type sondes, with the conversion from JMD sondes at Java in 1999, but there are variations in sonde preparation technique and data processing. During the 1998-1999 period, more than 550 sondes were incorporated into the SHADOZ data base. Examples from these measurements illustrate the tropical wave-one pattern in total ozone which is easily detectable by satellite. They also show that the wave-one pattern appears to be in the troposphere, as assumed in creating the modified-residual tropospheric ozone data product from TOMS. SHADOZ will add data from intensive field campaigns from time to time. Recent contributions to the SHADOZ archive are from the INDOEX (Indian Ocean Experiment January-March 1999)sondes at the Maldives (5N, 73E) and 27 sondes on the US NOAA oceanographic vessel, the FIN Ronald H Brown between Virginia (US) and Mauritius via CapeTown, during a cruise in January and February 1999. In 2000, as part of the SAFARI-2000 experiment and a validation project called Southern African Validation for EOS (SAVE), enhancement of ozonesonde launches at Irene (South Africa) will extend the data set from this site beyond the 1998-1999 period.

Thompson, Anne M.↗

Comparison of Observed Longwave, Shortwave Irradiance and Surface Temperature from “MOSAiC” to CERES Radiative Transfer Calculations and Inputs

As atmospheric temperatures rise due to increased anthropogenic forcing, the effect is expected to be larger over the arctic than midlatitude and tropics, known as polar amplification. A multi-national program, the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC) was ran between Sept 2019 and Oct 2020. The project deployed scientific instruments on board the German research vessel Polarstern with the ship remaining across a year to observe all aspects of polar climate. The US DOE deployed the ARM AMF2 aboard the ship along with several off-ship sites for extended spatial observations. Of particular importance was the measurement of the energetics of the ice/ocean/atmosphere interactions through observations of surface irradiance and surface temperatures. NASA’s Clouds and the Earth’s Radiant Energy System (CERES) project produces the SYN1deg Edition 4 data product. This product provides high quality, hourly, globally gridded and temporally complete maps of top of atmosphere (TOA), in atmosphere, and surface irradiances. TOA fluxes are derived from CERES instruments and geostationary satellites. In addition, TOA, in atmosphere, and surface irradiances are computed using the Langley Fu and Liou radiative transfer model. The radiative transfer model is run hourly at 1 degree ~equal area spatial resolution. Meteorological profiles are provided by Global Modeling and Assimilation Office’s GEOS-541 reanalysis product. Cloud properties are derived solely from Terra and Aqua MODIS imagers northward of 60°. Here we compare SYN1deg hourly calculations of surface irradiance to observations from the AMF2 to validate the products estimates of surface irradiance in this challenging area. Along with the irradiance comparisons we take a close look at the surface temperature record in the GEOS-541 product and compare it over both time and space to several surface observations provided by MOSAiC. Along with the comparison to the re-analysis record we will compare these observed surface temperatures to those derived from the AIRS product, which is known to have some difficulty in extreme high latitude areas. The goals of this study are 1) understand computed surface downward irradiance and temperature error separated by surface type (e.g. sea ice or open water) and by season, and 2) error covariance in spatial and temporal space. We seek to use this information to extrapolate the error from the MOSAiC domain to a larger arctic region.

David A Rutan↗

Low and High-Power Inductive Pulsed Plasma Thruster Development Testing at NASA-MSFC

The inductive pulsed plasma thruster (IPPT) is an electromagnetic plasma accelerator that has been identified in NASA roadmaps as an enabling propulsion technology for some niche low-power missions and for high-power in-space propulsion needs. The IPPT is an electrodeless space propulsion device where a capacitor is charged to an initial voltage and then discharged producing a high current pulse through a coil. The field produced by this pulse ionizes propellant, inductively driving current in a plasma located near the face of the coil. Once the plasma is formed it can be accelerated and expelled at a high exhaust velocity by the electromagnetic Lorentz body force arising from the interaction of the induced plasma current and the magnetic field produced by the current in the coil. Thrusters of this type possess many demonstrated and potential benefits that make them worthy of continued investigation. The electrodeless nature of these thrusters eliminates the lifetime and contamination issues associated with electrode erosion in conventional electric thrusters. Also, a wider variety of propellants are accessible when compatibility with metallic electrodes in no longer an issue. IPPTs have been successfully operated using propellants like ammonia, hydrazine, and CO2, and there is no fundamental reason why they would not operate on other in situ propellants like H2O. It is well-known that pulsed accelerators can maintain constant specific impulse (I(sub sp)) and thrust efficiency (eta(sub t)) over a wide range of input power levels by adjusting the pulse rate to hold the discharge energy per pulse constant. It has also been demonstrated that an inductive pulsed plasma thruster can operate in a regime where eta(sub t) is relatively constant over a wide range of I(sub sp) values (3000-8000 s). Finally, thrusters in this class have operated in single-pulse mode at high energy per pulse, and by increasing the pulse rate they offer the potential to process very high levels of power using a single thruster. There has been significant previous research on IPPTs designed around a planar-coil (flat-plate) geometry. The most notable of these was the Pulsed Inductive Thruster (PIT), with the PIT MkV presently representing the state-of- the-art in pulsed high-power IPPT technological development. In this paper, we focus on two planar-geometry devices that operate at significantly different power levels. Most work performed at NASA-Marshall Space Flight Center (MSFC) has, to date, focused on lower power thruster operation (approx. = 10s to 100s of J/pulse, up to 2-2.5 kW average power throughput) and previously described. The most recent work aimed to assemble a device that could be tested in cyclic mode on a thrust-stand, and which could augment the existing data set for IPPTs. In addition, the thruster was designed to serve as a test-bed for solid state switching circuitry and pulsed gas valves, with the modular design of the device allowing for variation in or upgrades to test configuration. Recently, MSFC obtained on loan from the Georgia Institute of Technology (Atlanta, GA) the PIT MkVI, successor to the PIT MkV. The MkV and MkVI are similar in design with much of the hardware from the former, specifically the capacitors and spark-gap switches, being reused in the latter. The coil is similar in geometry but has bent copper rods used in the latest iteration in place of the Litz wire windings found in the MkV. The MkVI master switch for the spark gaps is located in the vacuum chamber contained within a sealed, pressurized vessel fastened to the back of the thruster. This is different from the MkV where many capacitor charging lines and spark gap-triggering delay lines ran to the thruster from a master trigger located outside the vacuum chamber. The MkVI was damaged during testing soon after its fabrication was completed. The thruster arrived at MSFC still-damaged and mostly disassembled into many individual pieces. The device has been repaired, with a few additional design changes implemented after discussions with the late Prof. Lovberg regarding the initial testing results and issues encountered. In the present work, we present results from testing of both the small IPPT and the larger MkVI thruster. The smaller device (Fig. 1) is tested on a thrust stand on multiple gases to demonstrate its capability to operate in a repetition-rate mode and serve as a IPPT technology-development testbed. The larger MkVI (Fig. 2) is operated for the first time in its newly reconstituted state, demonstrating full-power pulsed operation and, for the first time, repetition-rate operation of a high-power IPPT. The additional upgrades required for synchronous operation of all the pulsed systems in single-pulse and repetition-rate mode are described in detail.

Polzin, Kurt A.↗

Inductive Pulsed Plasma Thruster Development Testing at NASA-MSFC

The inductive pulsed plasma thruster (IPPT) is an electromagnetic plasma accelerator that has been identified in NASA roadmaps as an enabling propulsion technology for some niche low-power missions and for high-power in-space propulsion needs. The IPPT is an electrodeless space propulsion device where a capacitor is charged to an initial voltage and then discharged producing a high current pulse through a coil. The field produced by this pulse ionizes propellant, inductively driving current in a plasma located near the face of the coil. Once the plasma is formed it can be accelerated and expelled at a high exhaust velocity by the electromagnetic Lorentz body force arising from the interaction of the induced plasma current and the magnetic field produced by the current in the coil. Thrusters of this type possess many demonstrated and potential benefits that make them worthy of continued investigation. The electrodeless nature of these thrusters eliminates the lifetime and contamination issues associated with electrode erosion in conventional electric thrusters. Also, a wider variety of propellants are accessible when compatibility with metallic electrodes in no longer an issue. IPPTs have been successfully operated using propellants like ammonia, hydrazine, and CO2, and there is no fundamental reason why they would not operate on other in situ propellants like H2O. It is well-known that pulsed accelerators can maintain constant specific impulse (I(sub sp)) and thrust efficiency (η(sub t)) over a wide range of input power levels by adjusting the pulse rate to hold the discharge energy per pulse constant. It has also been demonstrated that an inductive pulsed plasma thruster can operate in a regime where η(sub t) is relatively constant over a wide range of I(sub sp) values (3000-8000 s). Finally, thrusters in this class have operated in single-pulse mode at high energy per pulse, and by increasing the pulse rate they offer the potential to process very high levels of power using a single thruster. There has been significant previous research on IPPTs designed around a planar-coil (flat-plate) geometry. The most notable of these was the Pulsed Inductive Thruster (PIT), with the PIT MkV presently representing the state of- the-art in pulsed high-power IPPT technological development. In this paper, we focus on two planar-geometry devices that operate at significantly different power levels. Most work performed at NASA-Marshall Space Flight Center (MSFC) has, to date, focused on lower power thruster operation (≈ 10s to 100s of J/pulse, up to 2-2.5 kW average power throughput) and previously described in Refs. [5,6]. The most recent work aimed to assemble a device that could be tested in cyclic mode on a thrust-stand, and which could augment the existing data set for IPPTs. In addition, the thruster was designed to serve as a test-bed for solid state switching circuitry and pulsed gas valves, with the modular design of the device allowing for variation in or upgrades to test configuration. Recently, MSFC obtained on loan from the Georgia Institute of Technology (Atlanta, GA) the PIT MkVI, successor to the PIT MkV. The MkV and MkVI are similar in design with much of the hardware from the former, specifically the capacitors and spark-gap switches, being reused in the latter. The coil is simliar in geometry but has bent copper rods used in the latest iteration in place of the Litz wire windings found in the MkV. The MkVI master switch for the spark gaps is located in the vacuum chamber contained within a sealed, pressurized vessel fastened to the back of the thruster. This is different from the MkV where many capacitor charging lines and spark gap-triggering delay lines ran to the thruster from a master trigger located outside the vacuum chamber. The MkVI was damaged during testing soon after its fabrication was completed. The thruster arrived at MSFC still-damaged and mostly disassembled into many individual pieces. The device has been repaired, with a few additional design changes implemented after discussions with the late Prof. Lovberg regarding the initial testing results and issues encountered.

Polzin, Kurt A.↗

Development of an Inflatable Airlock for a Deep Space Gateway

Inflatable structures technology utilizes high-strength fabric materials and internal pressure to create a stiffened pressure vessel that can replace traditional metallic primary structure in a habitable spacecraft. The flexibility of fabric structures allows them to be compactly stowed for launch and expanded in space, providing significant launch volume savings. The unique construction and design flexibility of these structures can be customized for a variety of uses in space including landing bags, decelerators, long duration in-space and planetary surface habitats, and even airlocks. An airlock is often a required component of a crewed spacecraft to allow for maintenance and human exploration outside of the vehicle. Airlock designs in use today rely on complex hatches and seals connected by metallic walls. Recent developments towards the design of an inflatable airlock structure show feasibility and a significant launch volume savings over a traditional metallic design. This paper will provide a high-level summary of these projects and the current state-of-the-art in inflatable airlock development with additional references and detail about previous and on-going research, providing guidance for the design of a softgoods airlock system. The use of inflatables in space has been in development since the 1960’s for both habitats and airlocks. The first ever EVA was conducted by the USSR in 1965 using an inflatable airlock known as the Volga. This airlock was attached to the Voskhod 2 spacecraft and turned the vehicle into a dual chamber airlock. The airlock was successfully deployed, used and jettisoned after Alexey Leonov’s historic spacewalk. Additional work on human-rated inflatable structures was not continued until the late 1990’s when NASA-JSC led an effort to demonstrate these structures as feasible long-term pressurized elements with the TransHab project. The technology developed and pioneered during this project led to multiple patents and proven feasibility that inflatables could be used for large habitable structures. Following TransHab, Bigelow Aerospace continued the development of inflatable structures with technical support from NASA. This partnership eventually led to the successful flight certification, launch, attachment and deployment of the Bigelow Expandable Activities Module (BEAM) on the ISS in 2016. Inflatable and expandable airlock structures have undergone various detailed feasibility studies and testing for over 15 years, most notably with the Advanced Inflatable Airlock (AIA), Dual-Chamber Hybrid Inflatable Suitlock (DCIS), Minimalistic Advanced Soft Hatch (MASH), and Lightweight External Inflatable Airlock (LEIA). During this time, full-scale articles have been built and pressure-tested, and mock-ups and demonstrators have been constructed and evaluated. During the 2001-2003 timeframe, the AIA concept was matured through requirements development, conceptual design, subscale and full-scale engineering breadboards subjecting various test articles to deployment and pressure testing up to four times operating pressure. These tests proved the feasibility of successful deployment and structural integrity of an inflatable crewlock. Additional testing was performed in the ensuing years, as funding permitted, to further refine additional structural and deployment concepts and to understand the EVA crewmember interfaces, hatches and EVA support equipment interfaces that would be required for a fully functioning airlock. This work resulted in a refinement of the structural requirements and an accounting of the systems needed in an inflatable airlock. In 2014, the MASH project developed an ultra-lightweight airlock concept with a fabric hatch that utilized a unique pressure vessel shape to minimize structural loads around a linear seal. The concept uses an automated zipper-like seal that allows for crew egress/ingress. Most of the development work on the project thus far has focused on the design, analysis and testing of the primary structure and the zipper-like seal system is in the preliminary stages of development with a successful proof-of-concept test. As part of the 2017 LEIA effort, studies were conducted on EVA crewmember interfaces on the inside of an inflatable airlock. These efforts included the design of an internal secondary structure and placement of handholds and foot restraints to enable hatch opening, closing and translation through the airlock. Structural design, analysis and testing was completed on several secondary structure candidates. Crew interface testing was also completed using an inflatable crewlock mockup and the JSC Active Response Gravity Offload System (ARGOS) to simulate the movement of an EVA crewmember through an inflatable crewlock in microgravity. The results of these tests helped demonstrate the feasibility of utilizing an inflatable structure as an airlock and informed the required volume, hatch size, and configuration and location of translation aids for crewmembers in a microgravity crewlock. The ISS Quest airlock uses a dual-chamber design with isolated compartments known as the equipment-lock and the crewlock. The equipment-lock houses the Servicing, Performance and Checkout Equipment (SPCE) items (suit batteries, consumables, etc.) while the crewlock has limited internal hardware and is the nominally depressurized compartment during US EVAs. While inflatable dual chamber airlocks have been studied, the current state of the art emphasizes an inflatable crewlock-type structure attached to a rigid equipment-lock type or habitat structure. Since a large portion of the hardware in the equipment-lock are rigid components and connectors that are installed on the ground - and an inflatable structure does not achieve full structural capabilities until pressurized in space - a depressurized fabric structure cannot provide the capabilities of a full equipment-lock. The use of an inflatable as a crewlock, however, provides all the required capabilities for EVA operations in a small launch package that offers significant volume savings over a metallic crewlock. The functions of a traditional equipment lock, including the SPCE, could be provided by a spacecraft’s habitat module or node and not necessarily in a separate equipment lock. An inflatable crewlock would be attached to the vehicle and launched in a packed and compressed state, saving volume under the launch shroud and mass for the overall airlock element compared to a rigid crewlock. Work is currently underway to continue development of an inflatable airlock with a variety of focus areas including the consideration of crew-induced loads and interfaces, the design and development of an internal sub-structure to provide translation aids and restraints, the thermal considerations of a fabric shell depressurized during an EVA, the micrometeorite environment in deep space, and the packaging and deployment of an inflatable airlock.

Deep Space Exploration↗