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At least 37 records · Page 2

Gravitropism of hypocotyls of wild-type and starch-deficient Arabidopsis seedlings in spaceflight studies

The major purpose of this spaceflight project was to investigate the starch-statolith hypothesis for gravity perception, and a secondary goal was to study plant growth and development under spaceflight conditions. This research was based on our ground studies of gravity perception in the wild type and three starch-deficient (one starchless and two reduced starch) mutants of Arabidopsis thaliana (L.) Heynh. Dark-grown seedlings that developed in microgravity were given one of several (30 min, 60 min, or 90 min) 1-g stimuli by an on-board centrifuge, and additional controls for seedling development also were performed. These latter control experiments included a morphological study of plants that developed in space in microgravity (F microg), in space on a centrifuge (F 1g), on the ground (G 1g), and on a rotating clinostat on the ground. Since elevated levels of ethylene were reported in the spacecraft atmosphere, additional controls for morphology and gravitropism with added ethylene also were performed. While exogenous ethylene reduced the absolute magnitude of the response in all four strains of Arabidopsis, this gas did not appear to change the relative graviresponsiveness among the strains. The relative response of hypocotyls of microgravity-grown seedlings to the stimuli provided by the in-flight centrifuge was: wild type > starch-deficient mutants. Although the protoplast pressure model for gravity perception cannot be excluded, these results are consistent with a statolith-based model for perception in plants.

Non-NASA Center↗

Plastid sedimentation kinetics in roots of wild-type and starch-deficient mutants of Arabidopsis

Sedimentation and movement of plastids in columella cells of the root cap were measured in seedlings of wild-type, a reduced starch mutant, and a starchless mutant of Arabidopsis. To assay for sedimentation, we used both linear measurements and the change of angle from the cell center as indices in vertical and reoriented plants with the aid of computer-assisted image analysis. Seedlings were fixed at short periods after reorientation, and plastid sedimentation correlated with starch content in the three strains of Arabidopsis. Amyloplasts of wild-type seedlings showed the greatest sedimentation, whereas plastids of the starchless mutant showed no significant sedimentation in the vertically grown and reoriented seedlings. Because previous research has shown that a full complement of starch is needed for full gravitropic sensitivity, this study correlates increased sensitivity with plastid sedimentation. However, although plastid sedimentation contributed to gravisensitivity, it was not required, because the gravitropic starchless mutant had plastids that did not sediment. This is the first study, to our knowledge, to measure plastid sedimentation in Arabidopsis roots after reorientation of seedlings. Taken together, the results of this study are consistent with the classic plastid-based and protoplast-based models of graviperception and suggest that multiple systems of perception exist in plant cells.

NASA Discipline Plant Biology↗

The Mineralogy of Comet Wild-2 Nucleus Samples - What We Think We Know And What We Do Not Know

The sample return capsule of the Stardust spacecraft was successfully recovered in northern Utah on January 15, 2006, and its cargo of coma grains from Comet Wild-2 has now been the subject of intense investigation by approximately 200 scientists scattered across five continents. We can now perform mineralogical and petrographic analyses of particles derived directly from the Jupiter-family Comet Wild-2

Zolensky, Michael E.↗

SEM-EDS Analyses of Small Craters in Stardust Aluminum Foils: Implications for the Wild-2 Dust Distribution

Aluminium foils were used on Stardust to stabilize the aerogel specimens in the modular collector tray. Part of these foils were fully exposed to the flux of cometary grains emanating from Wild 2. Because the exposed part of these foils had to be harvested before extraction of the aerogel, numerous foil strips some 1.7 mm wide and 13 or 33 mm long were generated during Stardusts's Preliminary Examination (PE). These strips are readily accommodated in their entirety in the sample chambers of modern SEMs, thus providing the opportunity to characterize in situ the size distribution and residue composition - employing EDS methods - of statistically more significant numbers of cometary dust particles compared to aerogel, the latter mandating extensive sample preparation. We describe here the analysis of nearly 300 impact craters and their implications for Wild 2 dust.

Borg, J.↗

Synchrotron X-Ray Diffraction Studies of Olivine from Comet Wild 2

We have analyzed a collection of the Comet Wild 2 coma grains returned by the NASA Stardust Mission, using micro-area Laue diffraction equipment. The purpose of the diffraction experiment is to permit the structure refinement of olivine including site occupancies. In addition to the intrinsic importance of the olivine structures for revealing the thermal history of Wild 2 materials, we wish to test reports that olivine recovered after hypervelocity capture in silica aerogel has undergone a basic structural change due to capture heating [1]. The diffraction equipment placed at beam line BL- 4B1 of PF, KEK was developed with a micropinhole and an imaging plate (Fuji Co. Ltd.) using the Laue method combined with polychromatic X-ray of synchrotron radiation operated at energy of 2.5 GeV. The incident beam is limited to 1.6 m in diameter by a micropinhole set just upstream of the sample [2, 3]. It is essential to apply a microbeam to obtain diffracted intensities with high signal to noise ratios. This equipment has been successfully applied to various extraterrestrial materials, including meteorites and interplanetary dust particles [4]. The Laue pattern of the sample C2067,1,111,4 (Fig. 1) was successfully taken on an imaging plate after a 120 minute exposure (Fig. 2).

Source record↗

The Mineralogy of Comet Wild 2

The nature of cometary solids is of fundamental importance to our understanding of the early solar nebula and protoplanetary history. Samples of Comet Wild 2, provided by the Stardust Mission, have now been examined in terrestrial labs for two years, and are very surprising! Here we describe mainly the critical phases olivine, pyroxene and Fe-Ni sulfides in Wild 2 grains, as a guide to the general mineralogy of the returned comet samples.

Zolensky, Michael↗

Laboratory Simulation of Impacts upon Aluminum Foils of the Stardust Spacecraft: Calibration of Dust Particle Size from Comet Wild 2

Metallic aluminium alloy foils exposed on the forward, comet-facing surface of the aerogel tray on the Stardust spacecraft are likely to have been impacted by the same cometary particle population as the dedicated impact sensors and the aerogel collector. The ability of soft aluminium alloy to record hypervelocity impacts as bowl-shaped craters offers an opportunistic substrate for recognition of impacts by particles of a wide potential size range. In contrast to impact surveys conducted on samples from low Earth orbit, the simple encounter geometry for Stardust and Wild 2, with a known and constant spacecraft-particle relative velocity and effective surface-perpendicular impact trajectories, permits closely comparable simulation in laboratory experiments. For a detailed calibration programme we have selected a suite of spherical glass projectiles of uniform density and hardness characteristics, with well-documented particle size range from 10 microns to nearly 100 microns. Light gas gun buckshot firings of these particles at approximately 6km s)exp -1) onto samples of the same foil as employed on Stardust have yielded large numbers of craters. Scanning electron microscopy of both projectiles and impact features has allowed construction of a calibration plot, showing a linear relationship between impacting particle size and impact crater diameter. The close match between our experimental conditions and the Stardust mission encounter parameters should provide another opportunity to measure particle size distributions and fluxes close to the nucleus of Wild 2, independent of the active impact detector instruments aboard the Stardust spacecraft.

Kearsley, A. T.↗

Using the Fe/Mn Ratio of FeO-Rich Olivine In WILD 2, Chondrite Matrix, and Type IIA Chondrules to Disentangle Their Histories

The Stardust Mission returned a large abundance of impactors from Comet 81P/Wild2 in the 5-30 m range. The preliminary examination of just a limited number of these particles showed that the collection captured abundant crystalline grains with a diverse mineralogy [1,2]. Many of these grains resemble those found in chondrite matrix and even contain fragments of chondrules and CAIs [1-3]. In particular, the olivine found in Wild 2 exhibits a wide compositional range (Fa0-97) with minor element abundances similar to the matrix olivine found in many carbonaceous chondrites (CCs) and unequilibrated ordinary chondrites (UOCs). Despite the wide distribution of Fa content, the olivine found in the matrices of CCs, UOCs, and Wild 2 can be roughly lumped into two types based solely on fayalite content. In fact, in some cases, a distinct bi-modal distribution is observed.

Frank, David R.↗

Rift Valley Fever Risk Map Model and Seroprevalence in Selected Wild Ungulates and Camels from Kenya

Since the first isolation of Rift Valley fever virus (RVFV) in the 1930s, there have been multiple epizootics and epidemics in animals and humans in sub-Saharan Africa. Prospective climate-based models have recently been developed that flag areas at risk of RVFV transmission in endemic regions based on key environmental indicators that precede Rift Valley fever (RVF) epizootics and epidemics. Although the timing and locations of human case data from the 2006-2007 RVF outbreak in Kenya have been compared to risk zones flagged by the model, seroprevalence of RVF antibodies in wildlife has not yet been analyzed in light of temporal and spatial predictions of RVF activity. Primarily wild ungulate serum samples from periods before, during, and after the 2006-2007 RVF epizootic were analyzed for the presence of RVFV IgM and/or IgG antibody. Results show an increase in RVF seropositivity from samples collected in 2007 (31.8%), compared to antibody prevalence observed from 2000-2006 (3.3%). After the epizootic, average RVF seropositivity diminished to 5% in samples collected from 2008-2009. Overlaying maps of modeled RVF risk assessments with sampling locations indicated positive RVF serology in several species of wild ungulate in or near areas flagged as being at risk for RVF. Our results establish the need to continue and expand sero-surveillance of wildlife species Kenya and elsewhere in the Horn of Africa to further calibrate and improve the RVF risk model, and better understand the dynamics of RVFV transmission.

Seroprevalence↗

Sulfur and Oxygen Isotopic Analysis of a Cosmic Symplectite from a Comet Wild 2 Stardust Terminal Particle

Introduction: Analyses of comet 81P/Wild 2 samples re-turned from the Stardust mission have uncovered surprising simi-larities to meteoritic material, including the identification of inner solar system grains [1-3]. The TEM characterization of terminal particle (TP) 4 from Stardust track #147 revealed an assemblage consisting of symplectically intergrown pentlandite and nanocrys-talline maghemite coexisting with high-Ca pyroxene [4]. Mineral-ogically similar cosmic symplectites (COS) containing pentlandite and magnetite in the primitive Acfer 094 meteorite are highly de-pleted in 16O (17O, 18O ~ 180 per mille) [5-7]. This isotopic signature is proposed to record alteration with primordial solar nebula water. Conversely, the normal O isotopic composition of the Stardust COS indicates alteration by a different aqueous reservoir, perhaps on the comet [8]. In this study, we analyzed the Wild 2 COS for S isotopes to further constrain its origin. Experimental: Thin sections of TP4 (12 m) were produced and their mineralogy was thoroughly characterized by TEM. Two of the sections were analyzed for O isotopes by isotopic imaging in the JSC NanoSIMS 50L. The sample in one of the slices was completely consumed. The remaining material in the adjacent slice was analyzed simultaneously for 16O, 32S, 33S, 34S, and 56Fe16O in electron multipliers using a Cs+ primary ion beam. Quasi-simulta-neous arrival (QSA) can have a significant effect on S isotopic ra-tios when using electron multipliers, resulting in undercounting of 32S [9]. Canyon Diablo troilite (CDT) was measured numerous times to deduce a correction factor for QSA and ensure measure-ment reproducibility. Isotopic ratios are reported relative to CDT. Results and Discussion: The Wild 2 COS is enriched in the heavy S isotopes relative to CDT (33S = 6.5 +/- 1.6 per mille; 34S = 5.1 +/- 0.7 per mille; 1). The degree of 33S enrichment indicates mass-inde-pendent fractionation (MIF) with 33S = 3.9 +/- 1.7 per mille. MIF of S has been observed in some chondrules (33S up to 0.11per mille) [10], but this effect has not been identified in sulfides from carbonaceous chondrites [11] or IDPs [12]. S isotopic analysis of Stardust impact craters also did not reveal MIF or anomalies, save for one potential 32S-rich presolar sulfide [13]. Measurement errors on these impact craters were much larger than those in this study, however. MIF of S has been proposed to result from heterogeneities in the solar neb-ula from nucleosynthetic components [14] or photochemical irra-diation of solar nebula gas [10]. Presolar SiC grains are observed to have 32S enrichments [15, 16] contrary to the S isotopic compo-sition of the cometary COS. The S isotopic composition more likely reflects irradiation of nebular gas.

Nguyen, A. N.↗

Editing wild points in isolation - Fast agreement for reliable systems (Preliminary version)

Consideration is given to the intuitively appealing notion of discarding sensor values which are strongly suspected of being erroneous in a modified approximate agreement protocol. Approximate agreement with editing imposes a time bound upon the convergence of the protocol - no such bound was possible for the original approximate agreement protocol. This new approach is potentially useful in the construction of asynchronous fault tolerant systems. The main result is that a wild-point replacement technique called t-worst editing can be shown to guarantee convergence of the approximate agreement protocol to a valid agreement value. Results are presented for a four-processor synchronous system in which a single processor may be faulty.

Kearns, Phil↗

Stardust Imaging of Comet Wild 2: First Look

On 2 January 2004 during its historic flight to return cometary dust samples to earth, the STARDUST spacecraft flew within the coma of comet Wild 2 and also took 72 images where the surface was resolved during the flyby. A combination of long and short exposures was used to observe the jets and the surface. Comet Surface: The images revealed a planetary body, one not having a significant atmosphere, quite different from any other such body seen from other spacecraft. Surface depressions, potentially a combination of craters and vents, were not bowl-shaped but typically had steep walls and flattened floors. One depression considered to be a vent, the source of a jet, had a depth to diameter ratio of approx.0.4, with near vertical walls. Jets: At least 10 to possibly 20 jets were active during the flyby. Some were traced back to the surface where they seem to originate from the near vertical walls of depressions (vents) that were facing the sun, having the highest solar insolation.

Newburn, R.↗

Streaming Clumps Ejection Model and the Heterogeneous Inner Coma of Comet Wild 2

The conventional concept of cometary comae is that they are dominated by fine particulates released individually by sublimation of surface volatiles and subsequent entrainment in the near-surface gas. It has long been recognized that such particulates could be relatively large, with early estimates that objects perhaps up to one meter in size may be levitated from the surface of the typical cometary nucleus. However, the general uniformity and small average particulate size of observed comae and the relatively smooth, monotonic increases and decreases in particle density during the Giotto flythrough of comet Halley s coma in 1986 reinforced the view that the bulk of the particles are released at the surface, are fine-sized and inert. Jets have been interpreted as geometrically constrained release of these particulates. With major heterogeneities observed during the recent flythrough of the inner coma of comet Wild 2, these views deserve reconsideration.

Clark, B. C.↗

Rootcap structure in wild type and in a starchless mutant of Arabidopsis

Rootcaps of the wild type (WT) and of a starchless, gravitropic mutant (TC7) of Arabidopsis thaliana L. were examined by electron microscopy to identify cellular polarities with respect to gravity. In columella cells, nuclei are located proximally, and the nuclear envelope is continuous with endoplasmic reticulum (ER) that is in turn connected to nearby plasmodesmata. Impregnation of ER with osmium ferricyanide revealed numerous contacts between columella plastids and ER in both genotypes. ER is present mostly in the outer regions of the columella protoplast except in older columella cells that are developing into peripheral cells. In vertical roots, only columella cells that are intermediate in development (story 2 cells) have a higher surface density (S) of ER in the distal compared to proximal regions of the cell. The distal but not the proximal S of the ER is constant throughout columella development. Plastids are less sedimented in TC7 columella cells compared to those of the WT. It is hypothesized that plastid contact with the ER plays a role in gravity perception in both genotypes.

NASA Discipline Plant Biology↗

Gravitropism and development of wild-type and starch-deficient mutants of Arabidopsis during spaceflight

The "starch-statolith" hypothesis has been used by plant physiologists to explain the gravity perception mechanism in higher plants. In order to help resolve some of the controversy associated with ground-based research that has supported this theory, we performed a spaceflight experiment during the January 1997 mission of the Space Shuttle STS-81. Seedlings of wild-type (WT) Arabidopsis, two reduced-starch strains, and a starchless mutant were grown in microgravity and then given a gravity stimulus on a centrifuge. In terms of development in space, germination was greater than 90% for seeds in microgravity, and flight seedlings were smaller (60% in total length) compared to control plants grown on the ground and to control plants on a rotating clinostat. Seedlings grown in space had two structural features that distinguished them from the controls: a greater density of root hairs and an anomalous hypocotyl hook structure. However, the slower growth and morphological changes observed in the flight seedlings may be due to the effects of ethylene present in the spacecraft. Nevertheless, during the flight hypocotyls of WT seedlings responded to a unilateral 60 min stimulus provided by a 1-g centrifuge while those of the starch-deficient strains did not. Thus the strain with the greatest amount of starch responded to the stimulus given in flight and therefore, these data support the starch-statolith model for gravity sensing.

short duration↗