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At least 37 records · Page 2

Report of Amphibian Development Group

Amphibian and fish embryos are extremely well suited for studies on pattern specification, whereas other systems (e.g., avian or mammalian) might be just as well suited for studies on differentiation or growth. Those distinctions are important for at least two reasons: (1) More precise focus regarding underlying mechanisms is called for when those distinctions are made. That facilitates the formulation of specific models or hypotheses; and (2) stress effects (i.e., the effects of weightlessness on structures (e.g., bones) which normally bear a load) are distinguished as being indirect, in contrast to direct effects of microgravity, which would be expected to act on pattern specification. That is, direct gravity effects are distinguished from indirect stress effects.

Malacinski, G.↗

Response of amphibian egg non-yolk cytoplasm to gravity orientation

In order to study amphibian egg cytoplasmic organization and egg symmetrization at the molecular level, a library of seventeen monoclonal antibodies (MoAbs) against Xenopus laevis non-yolk egg proteins was produced. Several of these MoAbs react with non-yolk cytoplasmic antigens which are unevenly distributed in the fertile Xenopus egg.

Smith, R. C.↗

Amphibian fertilization and development in microgravity

An experiment investigating the effects of gravity on embryonic development in amphibians is proposed. The planned procedures for the preparation of the frog eggs for launching in the Space Shuttle, for the injection of the eggs with gonadotropin, for the insertion of the eggs into egg chambers, for the storage of one of the chambers in a microgravity area and the second into a centrifuge, and for the fertilization of the eggs are described. The later organogenesis, swimming behavior, cytoplasmic components, cellular formation, neural plate and archenteron expansion, and allometry and expansion of the organ systems will be examined. Normal morphology for embryos and tadpoles developing at microgravity and the formation of the neural plate opposite the sperm entry point meridian are predicted.

Souza, K. A.↗

The amphibian egg as a model system for analyzing gravity effects

Amphibian eggs provide several advantageous features as a model system for analyzing the effects of gravity on single cells. Those features include large size, readily tracked intracellular inclusions, and ease of experimental manipulation. Employing novel gravity orientation as a tool, a substantial data base is being developed. That information is being used to construct a three-dimensional model of the frog (Xenopus laevis) egg. Internal cytoplasmic organization (rather than surface features) are being emphasized. Several cytoplasmic compartments (domains) have been elucidated, and their behavior in inverted eggs monitored. They have been incorporated into the model, and serve as a point of departure for further inquiry and speculation.

Malacinski, G. M.↗

Understanding the organization of the amphibian egg cytoplasm - Gravitational force as a probe

A combination of hypergravity (centrifugation) and hypogravity (clinostat) studies have been carried out on amphibian (frog, Xenopus) eggs. The results reveal that the twinning caused by centrifugation exhibits substantial spawning to spawning variation. That variation can be attributed to the apparent viscosity of the egg's internal cytoplasm. Simulated hypogravity results in a relocation of the egg's third (horizontal) cleavage furrow, toward the equator. Substantial egg-to-egg variation is also observed in this 'cleavage effect'. For interpreting spaceflight data and for using G-forces as probes for understanding the egg's architecture the egg variation documented herein should be considered.

Neff, Anton W.↗

Amphibian Development in the Virtual Absence of Gravity

To test whether gravity is required for normal amphibian development, Xenopus laevis females were induced to ovulate aboard the orbiting Space Shuttle. Eggs were fertilized in vitro, and although early embryonic stages showed some abnormalities, the embryos were able to regulate and produce nearly normal larvae. These results demonstrate that a vertebrate can ovulate in the virtual absence of gravity and that the eggs can develop to a free-living stage.

Souza, Kenneth A.↗

Utricular otoconia of some amphibians have calcitic morphology

This report concerns the morphological features of otoconia removed from the inner ear of four amphibian species. Results from scanning electron microscopic examination are compared based on the site of origin. These results show that utricular otoconia have a mineral structure that mimics calcite, rather than the widely accepted idea that they are mineralized by calcium carbonate of the aragonite polymorph.

NASA Program Space Physiology and Countermeasures↗

Acceleration Measurements During Landings of a 1/5.5-Size Dynamic Model of the Columbia XJL-1 Amphibian in Smooth Water and in Waves: Langley Tank Model 208M, TED No. NACA 2336

A 1/5.5-size powered dynamic model of the Columbia XJL-1 amphibian was landed in Langley tank no. 1 in smooth water and in oncoming waves of heights from 2.1 feet to 6.4 feet (full-size) and lengths from 50 feet to 264 feet (full-size). The motions and the vertical accelerations of the model were continuously recorded. The greatest vertical acceleration measured during the smooth-water landings was 3.1g. During landings in rough water the greatest vertical acceleration measured was 15.4g, for a landing in 6.4-foot by 165-foot waves. The impact accelerations increased with increase in wave height and, in general, decreased with increase in wave length. During the landings in waves the model bounced into the air at stalled attitudes at speeds below flying speed. The model trimmed up to the mechanical trim stop (20 deg) during landings in waves of heights greater than 2.0 feet. Solid water came over the bow and damaged the propeller during one landing in 6.4-foot waves. The vertical acceleration coefficients at first impact from the tank tests of a 1/5.5-size model were in fair agreement with data obtained at the Langley impact basin during tests of a 1/2-size model of the hull.

Clement, Eugene P.↗

Tank Tests of a 1/7-Size Dynamic Model of the Grumman XJR2F-1 Amphibian to Determine the Effect of Slotted- and Split-Type Flaps on Take-Off Stability - NACA Model 212, TED No. NACA 2378

Additional tests of a 1/7-size model of the Grumman XJR2F-1 amphibian were made in Langley tank no. 1 to compare the behavior during take-off of the model equipped with split- and slotted-type flaps. The slotted flag had a large effect on locating the forward center-of-gravity limits for stable take-offs. Stable take-offs within the normal operating range of positions of the center of gravity could be made with the split flaps deflected 45deg or with the slotted flaps deflected less than 20deg. At flap deflections required for similar take-off stability, the use of split-flaps resulted lower take-off speeds than the use of slotted flaps. An increase in forward acceleration from 1.1 to 4.8 feet per second per second moved the center-of-gravity limit forward approximately 3-percent mean aerodynamic chord.

Land, Norman S.↗

Tank Tests of a 1/7-Size Powered Dynamic Model of the Grumman XJR2F-1 Amphibian: Spray Characteristics, Take-Off and Landing Stability in Smooth Water - Langley Tank Model 212, TED No. NACA 2378

Tests of a model of the XJR2F-Y amphibian were made in Langley tank no. to determine the spray characteristics and the take-off and landing stability. At a gross load of 22,000 pounds full size, spray entered the propeller disk only at a very narrow range of speeds. The spray striking the flaps was not excessive and no appreciable wetting of the tail surfaces was noted. The trim limits of stability appeared to be satisfactory and the upper-limit porpoising was not violent. The stable range of center-of-gravity locations with flaps set 20deg was well aft of the desired operating range. However, with flaps up, the forward limit was about 18 percent mean aerodynamic chord and the aft limit about 28.5 percent mean aerodynamic chord at a load of 26,000 pounds and with elevators deflected -10deg. Under these conditions the location of the step is considered satisfactory. Tests showed that the effect of water in the nose-wheel well would be to move the forward limit aft about 2-percent mean aerodynamic chord. Without ventilation of the main step, the model skipped during landing at most trims, but this skipping was not violent. With the ventilation, the model skipped lightly only at trims where the afterbody keel was approximately parallel to the water (around 7.5 deg).

Land, Norman S.↗

Tank Tests of 1/5.5-Scale Forward Dynamic Model of the Columbia XJL-1 Amphibian - Langley Tank Model 208, TED No. NACA 2336

Tests of a powered dynamic model of the Columbia XJL-1 amphibian were made in Langley tank no.1 to determine the hydrodynamic stability and spray characteristics of the basic hull and to investigate the effects of modifications on these characteristics. Modifications to the forebody chime flare, the step, and the afterbody, and an increase in the angle of incidence of the wing were included in the test program. The seaworthiness and spray characteristics were studied from simulated taxi runs in smooth and rough water. The trim limits of stability, the range of stable positions of the enter of gravity for take-off, and the landing stability were determined in smooth water. The aerodynamic lift, pitching moment, and thrust were determined at speeds up to take-off speed.

Havens, Robert F.↗

Acceleration Measurements During Landing in Rough Water of a 1/7-Scale Dynamic Model of Grumman XJR2F-1 Amphibian - Langley Tank Model 212, TED No. NACA 2378

Tests of a 1/7 size model of the Grumman XJR2F-1 amphibian were made in Langley tank no.1 to examine the landing behavior in rough water and to measure the normal and angular accelerations experienced by the model during these landings. All landings were made normal to the direction of wave advance, a condition assumed to produce the greatest accelerations. Wave heights of 4.4 and 8.0 inches (2.5 and 4.7 ft, full size) were used in the tests and the wave lengths were varied between 10 and 50 feet (70 and 350 ft, full size). Maximum normal accelerations of about 6.5g were obtained in 4.4 inch waves and 8.5g were obtained in 8.0 inch waves. A maximum angular acceleration corresponding to 16 radians per second per second, full size, was obtained in the higher waves. The data indicate that the airplane will experience its greatest accelerations when landing in waves of about 20 feet (140 ft, full size) in length.

Land, Norman S.↗

Full-Scale Hydrodynamic Evaluation of a Modified Navy J4F-2 Amphibian with a 0.425-Scale XP5M-1 Hull Bottom. TED No. NACA DE325

An investigation was made to evaluate the hydrodynamic qualities of a 0.425-scale model of the Navy XP5M-1 hull, which was installed on a modified Navy J4F-2 amphibian. Longitudinal and directional stability during take-off and landing, low-speed maneuverability, spray characteristics, and take-off performance were investigated. The behavior of the airplane in moderately rough water was also observed. The opinions of three pilots have been correlated with the data.

Land, Norman S.↗

Ecological connectivity and in-kind mitigation in a regulatory decision framework: A case study with an amphibian habitat specialist

Ecological connectivity is critical to the survival and long-term viability of populations but is often overlooked in regulatory frameworks. We integrated landscape-level processes into a mitigation strategy for impacts to aquatic resources on the U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR) in eastern Tennessee. Wetlands on the ORR, which contain significant breeding populations of the imperiled four-toed salamander (Hemidactylium scutatum) and tubercled rein orchid (Platanthera flava var. herbiola), will be impacted by construction of an environmental waste disposal facility under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Here, we used a modified Kepner-Tregoe decision analysis to select general mitigation options that balanced regulatory requirements and interest group perspectives. We emphasized habitat connectivity through models that prioritized an area's importance to natural area connectivity (centrality) and maintenance of population structure for an affected habitat specialist (four-toed salamanders). We also emphasized in-kind mitigation through the preservation and enhancement of ecologically similar resources and the translocation and establishment of a new subpopulation of four-toed salamanders elsewhere on the ORR. We ultimately released over 500 juvenile salamanders that originated from the impacted site into the chosen mitigation wetlands. By doing so under the constraints of a time-sensitive CERCLA remediation effort and exceeding its substantive requirements, this work underscores feasibility. Ecological connectivity and the conservation of species that are not afforded explicit regulatory processes can be effectively and efficiently integrated into environmental decision-making and land use planning.

54 ENVIRONMENTAL SCIENCES↗