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At least 37 records · Page 2

Novel End-to-End Molecular Biology Approach for Direct Nanopore 1D cDNA Sequencing of Reverse Transcribed mRNAs Purified from Cell Cultures by the NASA ISS WetLab2 SPM

Continued space bioscience research onboard the International Space Station (ISS) and future long-duration flight missions to the Moon or Mars will require the ability to conduct on-orbit molecular analysis of biological samples independently from Earth. In the last year two new molecular analytic technologies have been installed and the technologies demonstrated onboard the ISS: The Sample Prep Module (SPM) WetLab-2 (WL2) qRT-PCR toolbox and the Oxford Nanopore MinIon Biomolecule Sequencer. Here we describe protocol development and integration into existing ISS technology for end-to-end on-orbit biological sample processing and molecular analysis with real time results generated utilizing only field offline analytic software. For this experiment we isolated primary cells from bone marrow flushes of wild type B6129SF2 mice (Jackson Labs) long bones. The cell isolate was then processed using the SPM to produce total 147nanograms of RNA. The total RNA was purified to only messenger RNA (mRNA) and transferred to Smartcycler Thermocycle ISS kit consumable tube using Eppendorf gel loading pipette tips for further processing. Complementary first strand cDNA was synthesized using OLIGO dT priming followed by addition of SuperScript II Reverse Transcriptase and thermal cycling as per manufacturers instruction. All thermal cycling was conducted using the ISS WetLab-2 Cephid Smarcycler real time thermal cycler. Our protocol takes advantage of mRNAs native poly(A) tail, synthesized in vivo to protect the mRNA from degradation by endonucleases, to eliminate end-prep for adapter ligation. The adapted library is purified using MyOne C1 Streptavidin beads before elution in buffer. The pre-sequencing library is diluted in the loading buffer and injected into the MinIon sample port, drawn into the nanopore window by capillary action, and sequenced using the MinKnown software with local basecalling. The sequencing read produced 34.5 million events and local basecalling produced 117,301 successful reads. NCBI Blast of the data for the mouse genome resulted in 2,462 successful nucleotide collection matches (gene sequences) exceeding 70 homology. These results demonstrate the viability of this novel flight ready end-to-end sample analytic methodology and provide a real time homolog for flight experimentation utilizing supply kits and technologies that have already been demonstrated on ISS.

MinIon

End–to–End Metasurface Design for Temperature Imaging via Broadband Planck‐Radiation Regression

A theoretical framework is presented for temperature imaging from long-wavelength infrared (LWIR) thermal radiation (e.g., 8–12 µm) through the end-to-end design of a metasurface-optics frontend and a computational-reconstruction backend. A new nonlinear reconstruction algorithm, “Planck regression”, is introduced to reconstruct the temperature map from a gray scale sensor image, even in the presence of severe chromatic aberration, by exploiting black body and optical physics particular to thermal imaging. This algorithm is combined with an end-to-end approach that optimizes manufacturable, single-layer metasurfaces to yield the most accurate reconstruction. The designs demonstrate high-quality, noise-robust reconstructions of arbitrary temperature maps (including completely random images) in simulations of an ultra-compact thermal-imaging device. Here, it is also shown that Planck regression is much more generalizable to arbitrary images than a straightforward neural-network reconstruction, which requires a large training set of domain-specific images.

36 MATERIALS SCIENCE

End-to-end microgrid protection using distributed data-driven methods

This paper introduces an end-to-end microgrid protection framework that offers real-time system monitoring, fault-related decision making, and circuit breaker control. This is achieved through the design of distributed data-driven techniques based on the support vector machine method, where each relay is responsible for distributed data collection, fault detection, fault localization, and fault isolation. Local communication is established among neighboring relays, fostering cooperative fault localization and isolation. This decentralized design not only reduces the computational and communication requirements but also enables the adaptability of each relay under varying operational dynamics. The proposed end-to-end protection framework was validated using MATLAB/Simulink simulations on a 100% renewable microgrid, achieving an accuracy of 93.1% with response time of 0.0523 s, in protecting against a range of fault scenarios that are characterized by various types, locations, impedances, load conditions, photovoltaic power levels, and microgrid operating modes.

24 POWER TRANSMISSION AND DISTRIBUTION

End-to-end RMS error testing on a constant bandwidth FM/FM system

End-to-end root-mean-square (rms) tests performed on a constant bandwidth FM/FM system with various settings of system parameters are reported. The testing technique employed is that of sampling, digitizing, delaying, and comparing the analog input against the sampled and digitized corresponding output. Total system error is determined by fully loading all channels with band-limited noise and conducting end-to-end rms error tests on one channel. Tests are also conducted with and without a transmission link and plots of rms errors versus receiver signal-to-noise (S/N) values are obtained. The combined effects of intermodulation, adjacent channel crosstalk, and residual system noise are determined as well as the single channel distortion of the system.

Wallace, G. R.

Radiation from the open end of a cylindrical or conical pipe and scattering from the end of a rod or slab

The radiation of sound through the open end of a cylindrical or conical pipe of any cross section, or through a hole in a plane wall, is analyzed theoretically. The scattering of a sound wave by the end of a rod or slab is also treated. Only the case in which the wavelength is large compared with a typical radial dimension of the opening or of the end is considered. The method of matched asymptotic expansions is employed. Results on end corrections and reflection coefficients previously obtained by Helmholtz (1860), Rayleigh (1945), and Bazer and Karp (1954), using intuitive arguments, are recovered and verified. Agreement is found with the exact results of Levine and Schwinger (1948) and Vainstein (1948), as well as with the small radial-dimension/wavelength results of Lesser and Lewis (1972), in the cases they treated. In addition various new results are obtained.

Ting, L.

Radio science requirements and the end-to-end ranging system

Radio science ranging requirements negotiated between past and present flight projects and the DSN have generally focused on just the DSS and spacecraft hardware. All elements in the end-to-end system are analyzed and considered in terms of the error hierarchy. The end-to-end system is defined and examined as it applies to the generation of radio science ranging requirements. The variability of the performance levels of the system elements is emphasized with respect to the radio science experiment being performed and the DSN-spacecraft frequency band configuration.

Berman, A. L.

The NASA End-to-End Data System program

NASA has embarked on the NASA End-to-End Data System (NEEDS) program, the objective of which is to improve effectiveness and efficiency of NASA's data/information management system. Technology gaps and bottlenecks in the end-to-end system, which encompasses acquiring data, processing it, and converting it to useful information, will be identified. Technology development activities addressing these problem areas will be carried out and new technology will be implemented. Phase I, started in 1977, addresses some very near-term data handling and processing problems with the development of some subsystem components. Phase II, with a late 1978 start, will concentrate on complete subsystems developments to effect near real-time data management. Potential future phases are projected to revolve around the themes of low cost data distribution and a full system demonstration.

Price, R. D.

An end-to-end command and control concept for NASA data systems

Spacecraft command and control are currently taking on the characteristics of general computer-to-computer interprocess communication. The evolution of these systems during the 1980s will give NASA a true general-purpose end-to-end data request capability for the first time. This concept is presented in outline, with consideration of many of the detailed analyses and subsystem tradeoffs being performed as part of the NEEDS (NASA End-to-End Data System) program.

Desjardins, R.

User-oriented end-to-end transport protocols for the real-time distribution of telemetry data from NASA spacecraft

A set of standard telemetry protocols for downlink data flow facilitating the end-to-end transport of instrument data from the spacecraft to the user in real time is proposed. The direct switching of data by autonomous message 'packets' that are assembled by the source instrument on the spacecraft is discussed. The data system consists thus of a format on a message rather than word basis, and such packet telemetry would include standardized protocol headers. Standards are being developed within the NASA End-to-End Data System (NEEDS) program for the source packet and transport frame protocols. The source packet protocol contains identification of both the sequence number of the packet as it is generated by the source and the total length of the packet, while the transport frame protocol includes a sequence count defining the serial number of the frame as it is generated by the spacecraft data system, and a field specifying any 'options' selected in the format of the frame itself.

Hooke, A. J.

Image gathering, coding, and processing: End-to-end optimization for efficient and robust acquisition of visual information

Researchers are concerned with the end-to-end performance of image gathering, coding, and processing. The applications range from high-resolution television to vision-based robotics, wherever the resolution, efficiency and robustness of visual information acquisition and processing are critical. For the presentation at this workshop, it is convenient to divide research activities into the following two overlapping areas: The first is the development of focal-plane processing techniques and technology to effectively combine image gathering with coding, with an emphasis on low-level vision processing akin to the retinal processing in human vision. The approach includes the familiar Laplacian pyramid, the new intensity-dependent spatial summation, and parallel sensing/processing networks. Three-dimensional image gathering is attained by combining laser ranging with sensor-array imaging. The second is the rigorous extension of information theory and optimal filtering to visual information acquisition and processing. The goal is to provide a comprehensive methodology for quantitatively assessing the end-to-end performance of image gathering, coding, and processing.

Huck, Friedrich O.

Integrating end-to-end threads of control into object-oriented analysis and design

Current object-oriented analysis and design methodologies fall short in their use of mechanisms for identifying threads of control for the system being developed. The scenarios which typically describe a system are more global than looking at the individual objects and representing their behavior. Unlike conventional methodologies that use data flow and process-dependency diagrams, object-oriented methodologies do not provide a model for representing these global threads end-to-end. Tracing through threads of control is key to ensuring that a system is complete and timing constraints are addressed. The existence of multiple threads of control in a system necessitates a partitioning of the system into processes. This paper describes the application and representation of end-to-end threads of control to the object-oriented analysis and design process using object-oriented constructs. The issue of representation is viewed as a grouping problem, that is, how to group classes/objects at a higher level of abstraction so that the system may be viewed as a whole with both classes/objects and their associated dynamic behavior. Existing object-oriented development methodology techniques are extended by adding design-level constructs termed logical composite classes and process composite classes. Logical composite classes are design-level classes which group classes/objects both logically and by thread of control information. Process composite classes further refine the logical composite class groupings by using process partitioning criteria to produce optimum concurrent execution results. The goal of these design-level constructs is to ultimately provide the basis for a mechanism that can support the creation of process composite classes in an automated way. Using an automated mechanism makes it easier to partition a system into concurrently executing elements that can be run in parallel on multiple processors.

Mccandlish, Janet E.

Evaluation of NASA's end-to-end data systems using DSDS+

The Data Systems Dynamic Simulator (DSDS+) is a software tool being developed by the authors to evaluate candidate architectures for NASA's end-to-end data systems. Via modeling and simulation, we are able to quickly predict the performance characteristics of each architecture, to evaluate 'what-if' scenarios, and to perform sensitivity analyses. As such, we are using modeling and simulation to help NASA select the optimal system configuration, and to quantify the performance characteristics of this system prior to its delivery. This paper is divided into the following six sections: (1) The role of modeling and simulation in the systems engineering process. In this section, we briefly describe the different types of results obtained by modeling each phase of the systems engineering life cycle, from concept definition through operations and maintenance; (2) Recent applications of DSDS+. In this section, we describe ongoing applications of DSDS+ in support of the Earth Observing System (EOS), and we present some of the simulation results generated of candidate system designs. So far, we have modeled individual EOS subsystems (e.g. the Solid State Recorders used onboard the spacecraft), and we have also developed an integrated model of the EOS end-to-end data processing and data communications systems (from the payloads onboard to the principle investigator facilities on the ground); (3) Overview of DSDS+. In this section we define what a discrete-event model is, and how it works. The discussion is presented relative to the DSDS+ simulation tool that we have developed, including it's run-time optimization algorithms that enables DSDS+ to execute substantially faster than comparable discrete-event simulation tools; (4) Summary. In this section, we summarize our findings and 'lessons learned' during the development and application of DSDS+ to model NASA's data systems; (5) Further Information; and (6) Acknowledgements.

Rouff, Christopher

End-to-End System Test and Optical Performance Evaluation for the Solar and Heliosphere Observatory (SOHO) Ultraviolet Coronagraph Spectrometer (UVCS)

The UVCS is one of the instruments carried by the Solar and Heliospheric Observatory (SOHO), a joint NASA/ESA Spacecraft launched in November 1995. It is designed to perform ultraviolet spectroscopy and visible light polarimetry of the extended solar corona. The primary scientific objectives of the UVCS investigation are to study the physical processes occurring in the extended solar corona, such as: the mechanism of acceleration of the solar wind, the mechanism of coronal plasma heating, the identification of solar wind sources, and the investigation of the plasma properties of the solar wind. The UVCS End-to-End test activities included a comprehensive set of system level functional and optical tests. Although performed under severe schedule constraints, the End-to-End System Test was very successful and served to fully validate the UVCS optical design. All test results showed that the primary scientific objectives of the UVCS Mission were achievable.

Carosso, Paolo A.

End-to-End Demonstrator of the Safe Affordable Fission Engine (SAFE) 30: Power Conversion and Ion Engine Operation

The Safe Affordable Fission Engine (SAFE) test series addresses Phase 1 Space Fission Systems issues in particular non-nuclear testing and system integration issues leading to the testing and non-nuclear demonstration of a 400-kW fully integrated flight unit. The first part of the SAFE 30 test series demonstrated operation of the simulated nuclear core and heat pipe system. Experimental data acquired in a number of different test scenarios will validate existing computational models, demonstrated system flexibility (fast start-ups, multiple start-ups/shut downs), simulate predictable failure modes and operating environments. The objective of the second part is to demonstrate an integrated propulsion system consisting of a core, conversion system and a thruster where the system converts thermal heat into jet power. This end-to-end system demonstration sets a precedent for ground testing of nuclear electric propulsion systems. The paper describes the SAFE 30 end-to-end system demonstration and its subsystems.

Hrbud, Ivana

On-Orbit Performance Verification and End-To-End Characterization of the TDRS-H Ka-band Communications Payload

In June 2000, NASA launched the first of three next generation Tracking and Data Relay Satellites (TDRS-H) equipped with a Ka-band forward and return service capability. This Ka-band service supports forward data rates of up to 25 Mb/sec using the 22.55-23.55 GHz space-to-space allocation. Return services are supported via channel bandwidths of 225 and 650 MHz for data rates up to at least 800 Mb/sec using the 25.25 - 27.5 GHz space-to-space allocation. As part of NASA's acceptance of the TDRS-H spacecraft, an extensive on-orbit calibration, verification and characterization effort was performed to ensure that on-orbit spacecraft performance is within specified limits. This process verified the compliance of the Ka-band communications payload with all performance specifications, and demonstrated an end-to-end Ka-band service capability. This paper summarizes the results of the TDRS-H Ka-band communications payload on-orbit performance verification and end-to-end service characterization. Performance parameters addressed include antenna gain pattern, antenna Gain-to-System Noise Temperature (G/T), Effective Isotropically Radiated Power (EIRP), antenna pointing accuracy, frequency tunability, channel magnitude response, and Ka-band service Bit-Error-Rate (BER) performance.

Toral, Marco

On-Orbit Performance Verification and End-to-End Characterization of the TDRS-H Ka-Band Communications Payload

In June 2000, NASA launched the first of three next generation Tracking and Data Relay Satellites (TDRS-H) equipped with a Ka-band forward and return service capability. This Ka-band service supports forward data rates up to 25 Mb/sec using the 22.55 - 23.55 GHz space-to-space allocation. Return services are supported via channel bandwidths of 225 and 650 MHz for data rates up to 800 Mb/sec (QPSK) using the 25.25 - 27.5 GHz space-to-space allocation. As part of NASA's acceptance of the TDRS-H spacecraft, an extensive on-orbit calibration, verification and characterization effort was performed to ensure that on-orbit spacecraft performance is within specified limits. This process verified the compliance of the Ka-band communications payload with all performance specifications and demonstrated an end-to-end Ka-band service capability. This paper summarizes the results of the TDRS-H Ka-band communications payload on-orbit performance verification and end-to-end service characterization. Performance parameters addressed include Effective Isotropically Radiated Power (EIRP), antenna Gain-to-System Noise Temperature (G/T), antenna gain pattern, frequency tunability and accuracy, channel magnitude response, and Ka-band service Bit-Error-Rate (BER) performance.

Toral, Marco

An End-To-End Test of A Simulated Nuclear Electric Propulsion System

The Safe Affordable Fission Engine (SAFE) test series addresses Phase I Space Fission Systems issues in it particular non-nuclear testing and system integration issues leading to the testing and non-nuclear demonstration of a 400-kW fully integrated flight unit. The first part of the SAFE 30 test series demonstrated operation of the simulated nuclear core and heat pipe system. Experimental data acquired in a number of different test scenarios will validate existing computational models, demonstrated system flexibility (fast start-ups, multiple start-ups/shut downs), simulate predictable failure modes and operating environments. The objective of the second part is to demonstrate an integrated propulsion system consisting of a core, conversion system and a thruster where the system converts thermal heat into jet power. This end-to-end system demonstration sets a precedent for ground testing of nuclear electric propulsion systems. The paper describes the SAFE 30 end-to-end system demonstration and its subsystems.

VanDyke, Melissa

Development of a Dynamic, End-to-End Free Piston Stirling Convertor Model

A dynamic model for a free-piston Stirling convertor is being developed at the NASA Glenn Research Center. The model is an end-to-end system model that includes the cycle thermodynamics, the dynamics, and electrical aspects of the system. The subsystems of interest are the heat source, the springs, the moving masses, the linear alternator, the controller, and the end-user load. The envisioned use of the model will be in evaluating how changes in a subsystem could affect the operation of the convertor. The model under development will speed the evaluation of improvements to a subsystem and aid in determining areas in which most significant improvements may be found. One of the first uses of the end-toend model will be in the development of controller architectures. Another related area is in evaluating changes to details in the linear alternator.

Regan, Timothy F.