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At least 37 records · Page 2

Sunlight supply and gas exchange systems in microalgal bioreactor

The bioreactor with sunlight supply system and gas exchange systems presented has proved feasible in ground tests and shows much promise for space use as a closed ecological life support system device. The chief conclusions concerning the specification of total system needed for a life support system for a man in a space station are the following: (1) Sunlight supply system - compactness and low electrical consumption; (2) Bioreactor system - high density and growth rate of chlorella; and (3) Gas exchange system - enough for O2 production and CO2 assimilation.

Mori, K.↗

BOREAS TE-9 In Situ Diurnal Gas Exchange of NAS Boreal Forest Stands

The BOREAS TE-9 team collected several data sets related to chemical and photosynthetic properties of leaves in boreal forest tree species. The purpose of the BOREAS TE-09 study was threefold: 1) to provide in situ gas exchange data that will be used to validate models of photosynthetic responses to light, temperature, and carbon dioxide (CO2); 2) to compare the photosynthetic responses of different tree crown levels (upper and lower); and 3) to characterize the diurnal water potential curves for these sites to get an indication of the extent to which soil moisture supply to leaves might be limiting photosynthesis. The gas exchange data of the BOREAS NSA were collected to characterize diurnal gas exchange and water potential of two canopy levels of five boreal canopy cover types: young jack pine, old jack pine, old aspen, lowland old black spruce, and upland black spruce. These data were collected between 27-May-1994 and 17-Sep-1994. The data are provided in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

Hall, Forrest G.↗

Adaptations for gas exchange enabled the elongation of lepidopteran proboscises

The extensive biodiversification of butterflies and moths (Lepidoptera) is partly attributed to their unique mouthparts (proboscis [Pr]) that can span in length from less than 1 mm to over 280mm in Darwin's sphinx moths. Lepi-doptera, similar to other insects, are believed to inhale and exhale respiratory gases only through valve-like spi-racles on their thorax and abdomen, making gas exchange through the narrow tracheae (Tr) challenging for the elongated Pr. How Lepidoptera overcome distance effects for gas transport to the Pr is an open question that is important to understanding how the Pr elongated over evolutionary time. Here, we show with scanning electron microscopy and X-ray imaging that distance effects on gas exchange are overcome by previously unreported micropores on the Pr surface and by superhydrophobic Tr that prevent water loss and entry. We find that the density of micropores decreases monotonically along the Pr length with the maxima proportional to the Pr length and that micropore diameters produce a Knudsen number at the boundary between the slip and transition flow regimes. By numerical estimation, we further show that the respiratory gas exchange for the Pr predominantly occurs via diffusion through the micropores. In conclusion, these adaptations are key innovations vital to Pr elongation, which likely facilitated lepidopteran biodiversification and the radiation of angiosperms by coevolutionary processes.

59 BASIC BIOLOGICAL SCIENCES↗

Relationship between wind speed and gas exchange over the ocean

A quadratic dependence of gas exchange on wind speed is employed to analyze the relationship between gas transfer and wind speed with particular emphasizing variable and/or low wind speeds. The quadratic dependence is fit through gas-transfer velocities over the ocean determined by methods based on the natural C-14 disequilibrium and the bomb C-14 inventory. The variation in the CO2 levels is related to these mechanisms, but the results show that other causes play significant roles. A weaker dependence of gas transfer on wind is suggested for steady winds, and long-term averaged winds demonstrate a stronger dependence in the present model. The chemical enhancement of CO2 exchange is also shown to play a role by increasing CO2 fluxes at low wind speeds.

Wanninkhof, Rik↗

High Temperature Acclimation of Leaf Gas Exchange, Photochemistry, and Metabolomic Profiles in Populus trichocarpa

High temperatures alter the thermal sensitivities of numerous physiological and biochemical processes that impact tree growth and productivity. Foliar and root applications of methanol have been implicated in plant acclimation to high temperature via the C 1 pathway. Here, we characterized temperature acclimation at 35°C of leaf gas exchange, chlorophyll fluorescence and extractable metabolites of potted Populus trichocarpa saplings and examined potential influences of mM concentrations of methanol added during soil watering over a two-month period. Relative to plants grown under the low growth temperature (LGT), high growth temperature (HGT) plants showed a suppression of leaf water use and carbon cycling including transpiration (E), net photosynthesis (P n ), an estimate of photorespiration (R p ), and dark respiration (R d ), attributed to reductions in stomatal conductance and direct negative effects on gas exchange and photosynthetic machinery. In contrast, HGT plants showed an upregulation of non-photochemical quenching (NPQ t ), the optimum temperature for ETR, and leaf isoprene emissions at 40°C. A large number of metabolites (867) were induced under HGT, many implicated in flavonoid biosynthesis highlighting a potentially protective role for these compounds. Methanol application did not significantly alter leaf gas exchange but slightly reduced the suppression of R d and R p by the high growth temperature while slightly impairing ETR, Fv’/Fm’, and q p . However, we were unable to determine if soil methanol was sufficiently taken up by the plant to have a direct effect on foliar processes. A small number of extracted leaf tissue metabolites (55 out of 10,015) showed significantly altered abundances under LGT and methanol treatments relative to water controls, and this increased in compound number (222) at the HGT. The results demonstrate the large physiological and biochemical impacts of high growth temperature on poplar seedlings and highlights the enhancement of the optimum temperature of ETR as a rapid thermal acclimation mechanism. Finally, although no large effect on leaf physiology was observed, the results are consistent with methanol both impairing photochemistry of the light reactions via formaldehyde toxicity, and stimulating photosynthesis and dark respiration through formate oxidation to CO 2 .

59 BASIC BIOLOGICAL SCIENCES↗

Leaf gas exchange and fitted parameters, two sites in Panama, 2022

Photosynthetic CO2 response curves (ACi curves), light response curves (AQ curves), dark adapted dark respiration, conductance curves, and survey measurements for leaves measured in the Parque Natural Metropolitano (PNM) and Gamboa, Panama, from January to April 2022 are presented. Measurements were made on leaves from 46 different tree, shrub and liana species, from sunlit canopy and understory locations. Leaf area index from 8 vertical profiles at PNM are also included. The aim of this measurement campaign was three-fold: (i) to improve our understanding of the vertical variation in leaf-level water use efficiency; (ii) to develop an understanding of the sensitivity of stomata to changes in environmental conditions, especially light, humidity, and temperature; (iii) to improve models which can predict leaf traits from leaf contact spectral measurements. All the gas exchange data and metadata are presented in .csv files and complete instrument output are included in .zip folders. Data and metadata meet the ESS-DIVE leaf-level gas exchange reporting format requirements. The protocol details are provided as pdf documents. In addition to gas exchange data reported here these samples were also used for measurement of leaf optical properties, carbon and nitrogen content, and leaf mass per unit leaf area (LMA). These data can be cross linked using the unique sample ID and are provided in separate data packages.

54 ENVIRONMENTAL SCIENCES↗

Leaf gas exchange and fitted parameters, San Lorenzo, Panama, 2020

This data package contains photosynthetic CO2 response curves (ACi curves), light response curves (AQ curves), dark adapted dark respiration, conductance curves, and survey measurements for leaves measured at the San Lorenzo forest canopy crane site, Panama (PA-SLZ) from January to March 2020. Leaves were sampled from both the top of the canopy and multiple heights within the canopy from 10 vertical profiles and included around 50 species. In addition, six core species were also measured, from the top of the canopy. Fitted maximum carboxylation rate (Vcmax), maximum electron transport rate (Jmax), and triose phosphate utilization rate (TPU) were derived from measured data as well as the convexity, light use efficiency, apparent quantum yield, and Rdark. The measurements can also be used to derive the conductance parameters g0 and g1 of stomatal model. All the gas exchange data and metadata are presented in .csv files and complete instrument output are included in .zip folders. Data and metadata meet the ESS-DIVE leaf-level gas exchange reporting format requirements. The protocol details are provided as pdf documents. In addition to gas exchange data described here these samples were also used for measurement of leaf optical properties, carbon and nitrogen content, and leaf mass per unit leaf area (LMA) these data can be cross linked using the unique sample ID and are provided in separate related data packages. Sample information including canopy elevation and leaf area index (LAI) can be found in the related “Leaf and canopy traits” data package.

54 ENVIRONMENTAL SCIENCES↗

Scientific Verification Test of Orbitec Deployable Vegetable Production System for Salad Crop Growth on ISS- Gas Exchange System design and function

The ability to produce and maintain salad crops during long term missions would be a great benefit to NASA; the renewable food supply would save cargo space, weight and money. The ambient conditions of previous ground controlled crop plant experiments do not reflect the microgravity and high CO2 concentrations present during orbit. It has been established that microgravity does not considerably alter plant growth. (Monje, Stutte, Chapman, 2005). To support plants in a space-craft environment efficient and effective lighting and containment units are necessary. Three lighting systems were previously evaluated for radish growth in ambient air; fluorescent lamps in an Orbitec Biomass Production System Educational (BPSE), a combination of red, blue, and green LED's in a Deployable Vegetable Production System (Veggie), and a combination of red and blue LED's in a Veggie. When mass measurements compared the entire possible growing area vs. power consumed by the respective units, the Veggies clearly exceeded the BPSE indicating that the LED units were a more resource efficient means of growing radishes under ambient conditions in comparison with fluorescent lighting. To evaluate the most productive light treatment system for a long term space mission a more closely simulated ISS environment is necessary. To induce a CO2 dense atmosphere inside the Veggie's and BPSE a gas exchange system has been developed to maintain a range of 1000-1200 ppm CO2 during a 21-day light treatment experiment. This report details the design and function of the gas exchange system. The rehabilitation, trouble shooting, maintenance and testing of the gas exchange system have been my major assignments. I have also contributed to the planting, daily measurements and harvesting of the radish crops 21-day light treatment verification test.

Eldemire, Ashleigh↗

Consistent responses of vegetation gas exchange to elevated atmospheric CO 2 emerge from heuristic and optimization models

Elevated atmospheric CO 2 concentration is expected to increase leaf CO 2 assimilation rates, thus promoting plant growth and increasing leaf area. It also decreases stomatal conductance, allowing water savings, which have been hypothesized to drive large-scale greening, in particular in arid and semiarid climates. However, the increase in leaf area could reduce the benefits of elevated CO 2 concentration through soil water depletion. The net effect of elevated CO 2 on leaf- and canopy-level gas exchange remains uncertain. To address this question, we compare the outcomes of a heuristic model based on the Partitioning of Equilibrium Transpiration and Assimilation (PETA) hypothesis and three model variants based on stomatal optimization theory. Predicted relative changes in leaf- and canopy-level gas exchange rates are used as a metric of plant responses to changes in atmospheric CO 2 concentration. Both model approaches predict reductions in leaf-level transpiration rate due to decreased stomatal conductance under elevated CO 2 , but negligible (PETA) or no (optimization) changes in canopy-level transpiration due to the compensatory effect of increased leaf area. Leaf- and canopy-level CO 2 assimilation is predicted to increase, with an amplification of the CO 2 fertilization effect at the canopy level due to the enhanced leaf area. The expected increase in vapour pressure deficit (VPD) under warmer conditions is generally predicted to decrease the sensitivity of gas exchange to atmospheric CO 2 concentration in both models. The consistent predictions by different models that canopy-level transpiration varies little under elevated CO 2 due to combined stomatal conductance reduction and leaf area increase highlight the coordination of physiological and morphological characteristics in vegetation to maximize resource use (here water) under altered climatic conditions.

54 ENVIRONMENTAL SCIENCES↗

Gas exchange on Mono Lake and Crowley Lake, California

Gas exchange coefficients (k) have been determined for freshwater Crowley Lake and saline Mono Lake through the use of a man-made purposefully injected gas, SF6. The concentration decreased from an initial value of 40 to 4 pmol/L for Mono Lake and from 20 to 1 pmol/L for Crowley lake over a period of 6 wks. Wind-speed (u) records from anemometers on the shore of each lake made it possible to determine the relationship between k and u. The average u and k values for the experiment were identical for the two lakes, despite the large chemical differences. It is estimated that, for the u values observed over Mono Lake from July to December 1984, the exchange of CO2 occurred 2.5 times faster than without chemical enhancement. This is a factor of 4 lower than needed to explain the high invasion rate of C-14 produced by nuclear bomb tests.

Wanninkhof, Rik↗

Diurnal leaf gas exchange survey, Feb2016-May2016, PA-SLZ, PA-PNM: Panama

This data package contains the results of a diurnal leaf gas exchange survey measured on sunlit canopy trees within the NGEE Tropics sites Parque Natural Metropolitano (PA-PNM) and Bosque Protector San Lorenzo (PA-SLZ) in Panama. Measurements were taken on a monthly basis from February to May of 2016. This data was collected as part of the 2016 El Niño-Southern Oscillation (ENSO) campaign. Included in this data package are two Excel files with data (2016ENSO_Panama_DiurnalGasEx, 2016ENSO_Panama_AreaCorrections) and additional Excel files with associated metadata. Also included is a Word document (Metadata_description_2016_ENSO_Panama) with details such as data collection methods, equipment used, and site information. See related datasets for further sample details, leaf water potential, LMA, leaf spectra, other gas exchange and leaf chemistry. VERSION 2 update. The identification of a species from the PNM site has been corrected as follows: the identification of the tree initially identified as Pseudosamanea guachapele (ALBIED) has been revised to Albizia adinocephala (ALBIAD). The updated data package includes revised data, metadata and protocol documents updated to reflect this change.

54 ENVIRONMENTAL SCIENCES↗

CO2 response (ACi) gas exchange, calculated Vcmax & Jmax parameters, Feb2016-May2016, PA-SLZ, PA-PNM: Panama

This data package contains CO2 response (ACi) gas exchange and fitted Vcmax and Jmax parameters measured on sunlit canopy trees within the NGEE Tropics sites Parque Natural Metropolitano (PA-PNM) and Bosque Protector San Lorenzo (PA-SLZ) in Panama. Measurements were taken on a monthly basis from February to May of 2016. This data was collected as part of the 2016 El Niño-Southern Oscillation (ENSO) campaign. Included in this data package are two Excel files with data (2016ENSO_Panama_ACi, 2016ENSO_Panama_Fitted_Vcmax_Jmax) and three Excel files with associated metadata. Also included is a Word document (Metadata_description_2016_ENSO_Panama) with details such as data collection methods, equipment used, and site information and a pdf (NGEE_Tropics_ENSO_Aci_Protocol_V2). See related datasets for further sample details, leaf water potential, LMA, leaf spectra, diurnal gas exchange and leaf chemistry. VERSION 2 update. The identification of a species from the PNM site has been corrected as follows: the identification of the tree initially identified as Pseudosamanea guachapele (ALBIED) has been revised to Albizia adinocephala (ALBIAD). The updated data package includes revised data, metadata and protocol documents updated to reflect this change.

54 ENVIRONMENTAL SCIENCES↗

Operation of an ADR Using Helium Exchange Gas as a Substitute for a Failed Heat Switch

The Soft X-ray Spectrometer (SXS) is one of four instruments on the Japanese Astro-H mission, which is currently planned for launch in late 2015. The SXS will perform imaging spectroscopy in the soft X-ray band (0.3-12 keV) using a 6 6 pixel array of microcalorimeters cooled to 50 mK. The detectors are cooled by a 3-stage adiabatic demagnetization refrigerator (ADR) that rejects heat to either a superfluid helium tank (at 1.2 K) or to a 4.5 K Joule-Thomson (JT) cryocooler. Four gas-gap heat switches are used in the assembly to manage heat flow between the ADR stages and the heat sinks. The engineering model (EM) ADR was assembled and performance tested at NASA/GSFC in November 2011, and subsequently installed in the EM dewar at Sumitomo Heavy Industries, Japan. During the first cooldown in July 2012, a failure of the heat switch that linked the two colder stages of the ADR to the helium tank was observed. Operation of the ADR requires some mechanism for thermally linking the salt pills to the heat sink, and then thermally isolating them. With the failed heat switch unable to perform this function, an alternate plan was devised which used carefully controlled amounts of exchange gas in the dewar's guard vacuum to facilitate heat exchange. The process was successfully demonstrated in November 2012, allowing the ADR to cool the detectors to 50 mK for hold times in excess of 10 h. This paper describes the exchange-gas-assisted recycling process, and the strategies used to avoid helium contamination of the detectors at low temperature.

Adiabatic demagnetization↗

Vasomotor tone does not affect perfusion heterogeneity and gas exchange in normal primate lungs during normoxia

To determine whether vasoregulation is an important cause of pulmonary perfusion heterogeneity, we measured regional blood flow and gas exchange before and after giving prostacyclin (PGI(2)) to baboons. Four animals were anesthetized with ketamine and mechanically ventilated. Fluorescent microspheres were used to mark regional perfusion before and after PGI(2) infusion. The lungs were subsequently excised, dried inflated, and diced into approximately 2-cm(3) pieces (n = 1,208-1,629 per animal) with the spatial coordinates recorded for each piece. Blood flow to each piece was determined for each condition from the fluorescent signals. Blood flow heterogeneity did not change with PGI(2) infusion. Two other measures of spatial blood flow distribution, the fractal dimension and the spatial correlation, did not change with PGI(2) infusion. Alveolar-arterial O(2) differences did not change with PGI(2) infusion. We conclude that, in normal primate lungs during normoxia, vasomotor tone is not a significant cause of perfusion heterogeneity. Despite the heterogeneous distribution of blood flow, active regulation of regional perfusion is not required for efficient gas exchange.

Non-NASA Center↗

BOREAS TE-10 Leaf Gas Exchange Data

The Boreal Ecosystem-Atmospheric Study (BOREAS) TE-10 (Terrestrial Ecology) team collected several data sets in support of its efforts to characterize and interpret information on the reflectance, transmittance, gas exchange, chlorophyll content, carbon content, hydrogen content, and nitrogen content of boreal vegetation. This data set contains measurements of assimilation, stomatal conductance, transpiration, internal CO2 concentration, and water use efficiency conducted in the Southern Study Area (SSA) during the growing seasons of 1994 and 1996 using a portable gas exchange system. The data are stored in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

Hall, Forrest G.↗

Respiratory gas exchange of high altitude adapted chick embryos

Study of gas exchange by embryos from chickens acclimatized to an altitude of 3800 m. The oxygen partial pressure and carbon dioxide partial pressure differences across the egg shell were measured and found to be less than the values previously reported for sea-level eggs by about a factor of two. Further measurements of embryonic oxygen consumption and shell conductivity to oxygen indicated that, compared to eggs at sea level, oxygen consumption was reduced by a factor of 0.58 while conductivity to oxygen was increased only by a factor of 1.07 in the high-altitude eggs. These independent measurements predict the change in oxygen partial pressure across the egg shell of the high-altitude eggs to be only 0.54 times that of sea-level eggs; the directly measured factor was 0.53. The authors conclude that at high altitude, a major adaptation of the chick embryo is a reduced metabolism which decreases the change in oxygen partial pressure across the egg shell since its gas conductivity remains essentially unchanged.

Wangensteen, O. D.↗