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27 records · Page 2
All sourcesNASA NTRSDOE OSTI
DOE OSTI2018

Evolution of Optical Diffraction Patterns on Disordered Woodpile Photonic Structures

Using three-dimensional direct laser writing, ordered and disordered photonic woodpile structures have been produced. An ideal woodpile is formed by layers of parallel “logs” turned through 90° with respect to logs of the previous layer. The disorder was specified by a random deviation in the angle with respect to their parallel arrangement in each layer of the woodpile. The quality of samples was tested by scanning electron microscopy. The optical diffraction patterns were studied experimentally on microsamples with different degrees of disorder and structure periods. With an increase in the degree of the disorder, the diffraction patterns changed qualitatively with the preservation of the zero diffraction order and formation of a speckle field pattern by higher diffraction orders.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
NASA NTRS1982

Microchrons - The Rb-87/Sr-87 dating of microscopic samples

The present investigation is concerned with the extension of the Rb-Sr dating technique to microscopic samples of individual mineral grains as small as approximately 30 micrometers in size. The measurement of the isotopic composition and the relative number of atoms of Rb and Sr has presented difficulties because of the need to separate Rb from Sr due to the isobaric interference at mass 87. This separation is critical since the changes in Sr-87/Sr-86 are directly related to the age of the sample. The approach used in the considered investigation is a modified version of the direct loading technique reported by Wasserburg et al. (1977). It is shown that measurements can be conducted of the Rb and Sr isotopic compositions and Rb/Sr atom ratios in microsamples containing 10 to the 11th to 10 to the 12th atoms of Sr. Rb-Sr model ages may prove to be of considerable importance in identifying and studying interplanetary dust particles.

Papanastassiou, D. A.↗
NASA NTRS1983

Chemical and isotopic study of extraterrestrial particles from the ocean floor

An Rb-Sr isotopic analysis of deep-sea spherules (DSS) from the Pacific, vesicular iridium-rich glassy objects (VIRGO) from an Antarctic-Ocean diatomaceous-ooze core, and equigranular objects (EGO) associated with or included in VIRGO, is reported. The techniques of sample collection, preparation, chemical analysis, and isotopic analysis (by mass spectrometry) are described in detail, and the results are presented in tables. The Sr-87/Sr-86 determination has a precision of 1-2 percent in microsamples of 2-8 x 10 to the 11th Sr atoms. The DSS are found to have Sr concentrations and Sr-87/Sr-86 (0.730-0.757) typical of chondritic meteorites. Extreme Rb depletion is attributed to volatization during meteoroid atmospheric heating. The VIRGO, while having major-element compositions like that of meteorites and high Ir levels suggesting extraterrestrial origin, have Sr-87/Sr-86 = 0.703-0.705; and the associated EGO has Sr-87/Sr-86 = 0.701 + or - 0.001, typical of MORB or basaltic achondrites. Hence their extraterrestrial origin is not proved, and some lithic component other than the EGO must be the source of the high Ir concentration.

Papanastassiou, D. A.↗
NASA NTRS1990

On the determination of iridium in diverse geological samples employing HPGe-coincidence/NaI(Tl)-anticoincidence spectrometry

This paper reports the Ir content of a variety of geological samples determined by the high-purity Ge-coincidence/NaI(Tl)-anticoincidence gamma-ray spectrometry (henceforth referred to as coincidence/anticoincidence technique) and by the conventional INAA. The advantages of this technique are: (1) the Ir content of the samples is obtained (ppm to a fraction of ppb ranges) not only by the 468.1 keV peak as in the conventional INAA but also by the 784.6 keV and 920.9 keV sum peaks, which gives more confidence in the values obtained; and (2) it is well suited for the samples with high Compton background for which it is difficult to measure the Ir content by the conventional INAA technique. The practical sensitivity of this technique depends on the sample matrix. Under present experimental conditions, it varied from 0.1 ng for Mn nodules and 0.004 ng for Libyan Desert Glass. Iridium values obtained on small (about 1 microg) olivine grains demonstrate the potential application of this new technique to microsamples. The principle and methodology of this new technique as well as its advantages and disadvantages over the conventional INAA are discussed.

Murali, A. V.↗
NASA NTRS2007

Antibodies Against Three Forms of Urokinase

Antibodies that bind to preselected regions of the urokinase molecule have been developed. These antibodies can be used to measure small quantities of each of three molecular forms of urokinase that could be contained in microsamples or conditioned media harvested from cultures of mammalian cells. Previously available antibodies and assay techniques do not yield both clear distinctions among, and measurements of, all three forms. Urokinase is a zymogen that is synthesized in a single-chain form, called ScuPA, which is composed of 411 amino acid residues (see figure). ScuPA has very little enzyme activity, but it can be activated in two ways: (1) by cleavage of the peptide bond lysine 158/isoleucine 159 and the loss of lysine 158 to obtain the high molecular-weight (HMW) form of the enzyme or (2) by cleavage of the bond lysine 135/lysine 136 to obtain the low-molecular-weight (LMW) form of the enzyme. The antibodies in question were produced in mice and rabbits by use of peptides as immunogens. The peptides were selected to obtain antibodies that bind to regions of ScuPA that include the lysine 158/isoleucine 159 and the lysine 135/lysine 136 bonds. The antibodies include monoclonal and polyclonal ones that yield indications as to whether either of these bonds is intact. The polyclonal antibodies include ones that preferentially bind to the HMW or LMW forms of the urokinase molecule. The monoclonal antibodies include ones that discriminate between the ScuPA and the HMW form. A combination of these molecular-specific antibodies will enable simultaneous assays of the ScuPA, HMW, and LMW forms in the same specimen of culture medium.

Morrison, Dennis R.↗
NASA NTRS2018

Advanced Curation Development of Tools and Methods for Microparticle Curation

The Astromaterials Acquisition and Curation Office at NASA Johnson Space Center is currently developing new tools and methods for the collection, storage, handling and characterization of particles less than 100 microns in diameter, or microparticles. Astromaterials Curation currently maintains four microparticle collections: Cosmic Dust that has been collected in Earth’s stratosphere by ER2 and WB-57 aircraft, Comet 81P/Wild 2 dust returned by NASA’s Stardust spacecraft, interstellar dust that was returned by Stardust, and asteroid Itokawa particles that were returned by the JAXA (Japan Aerospace Exploration Agency)’s Hayabusa spacecraft. NASA Curation is currently preparing for the anticipated return of two new astromaterials collections – asteroid Ryugu regolith to be collected by Hayabusa II spacecraft in 2021 (samples will be provided by JAXA as part of an international agreement), and asteroid Bennu regolith to be collected by the OSIRIS-REx (Origins Spectral Interpretation Resource Identification Security - Regolith Explorer) spacecraft in 2023. In order to maximize the scientific yield from these valuable acquisitions, it will be necessary to develop methods that extend our current microsample handling capabilities. Here we describe recent progress in the development of sample handling techniques that will enhance our microparticle curation capabilities. Further described are: Six-Axis Robot Arms for Particle Manipulation, and Charge-Dissipative Substrates.

Snead, Christopher↗
NASA NTRSDate not supplied

Center for Isotope Cosmochemistry and Geochronology at NASA Johnson Space Center

Ten years in the making the Center for Isotope Cosmochemistry and Geochronology (CICG) at NASA Johnson Space Center is designed to be a cooperative analytical facility where novel techniques are developed and performed. Our team works collaboratively to operate this integrated lab suite comprising sample preparation, metal-free clean chemistry, and mass spectrometers to measure a wide variety of elements and isotopes in astromaterials. Contamination control is enacted throughout by a positive pressure cascade of HEPA filtered air, weekly surface cleaning and particle counting. Astromaterials processing is performed with application-specific mortar and pestles, heavy liquid separation, a Frantz magnetic separator, as well as hand-picking using optical microscopes in laminar flow boxes. Microsampling is also carried out using a New Wave MicroMill. Elements for isotopic analysis are isolated from acid-digested samples using ion-exchange chromatography. Cross-contamination is minimized with dedicated laminar flow boxes and labware for different purification protocols. Instrumentation includes a ThermoFisher Element XR inductively coupled plasma mass spectrometer (ICP-MS) for quantification of elemental concentrations in dissolved sample solutions. For in situ analysis, a Teledyne Photon Machines Analyte Excite+ laser ablation system is coupled to the Element XR. The ThermoFisher Triton thermal ionization mass spectrometer (TIMS) is used to measure the isotopic composition of elements such as CA, K, Cr, Rb, Sr, Sm, Nd at high precision in processed samples. For a more diverse range of isotopic analyses, CICG houses a Nu Sapphire 1700 multi-collector inductively coupled plasma mass spectrometer (MC-ICP-MS), which is a large geometry instrument with a multi-pole collision cell for interference removal. An Applied Spectra Instruments J200 femto-second laser is planned for install this Fall and will be coupled to the Nu Sapphire 1700. NASA’s Internal Scientist Funding Model (ISFM)supports this facility. Advanced analytical geochemistry techniques are applied to targeted astromaterials to address important problems in planetary sciences, with a longer-term goal of preparing for samples collected and returned by space missions. CICG is open to collaborations on projects that utilize the facility’s unique capabilities and are within its research scope. We are particularly eager to support and share expertise with the next generation of planetary scientists.

Justin I Simon↗
DOE OSTI2024

A Methodology for determining optimal milling parameters for low-taper microtensile sample production

Femtosecond lasers are beginning to see an increase in interest for industrial and high throughput microsample milling applications. Despite this, much of the literature regarding removal rate studies for ultrashort pulse laser milling continues to focus on single-pulse or similar experiments that have a very small time on target. The material volume removal rates of femtosecond milling can vary wildly depending on parameters like laser scan speed, hatch spacing, z-axis step size, and pulses on the material. Currently, there is no established methodology for determining the optimal values of these parameters for micromilling applications. This methodology was developed in this study. The methodology consists of a pulse study, a repeat study, and a z-step study that will return the milling parameters that resulted in the largest volume removal rate for the laser used for this research. The methodology was created for the laser system used in this study, and was tested on 316 Stainless Steel, but was developed in such a way that it may be adapted to any material, and with some tweaking of variables might be used in other laser systems. The laser system used in this study utilizes a novel positioning stage that introduces 6 degrees of freedom to the translation of laser samples, being able to tilt and move in all three axes. Using both the developed methodology and the unique capabilities of the laser microtensile samples were produced with taper angles of <1° in 316 Stainless Steel.

Barker, Zachary Wayne↗