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At least 37 records · Page 2

Cryo-ET of Toxoplasma parasites gives subnanometer insight into tubulin-based structures

Tubulin is a conserved protein that polymerizes into different forms of filamentous structures in Toxoplasma gondii, an obligate intracellular parasite in the phylum Apicomplexa. Two key tubulin-containing cytoskeletal components are subpellicular microtubules (SPMTs) and conoid fibrils (CFs). The SPMTs help maintain shape and gliding motility, while the CFs are implicated in invasion. Here, we use cryogenic electron tomography to determine the molecular structures of the SPMTs and CFs in vitrified intact and detergent-extracted parasites. Subvolume densities from detergent-extracted parasites yielded averaged density maps at subnanometer resolutions, and these were related back to their architecture in situ. An intralumenal spiral lines the interior of the 13-protofilament SPMTs, revealing a preferred orientation of these microtubules relative to the parasite’s long axis. Each CF is composed of nine tubulin protofilaments that display a comma-shaped cross-section, plus additional associated components. Conoid protrusion, a crucial step in invasion, is associated with an altered pitch of each CF. The use of basic building blocks of protofilaments and different accessory proteins in one organism illustrates the versatility of tubulin to form two distinct types of assemblies, SPMTs and CFs.

59 BASIC BIOLOGICAL SCIENCES↗

Analysis and mitigation of parasitic resistance effects for analog in-memory neural network acceleration

To support the increasing demands for efficient deep neural network processing, accelerators based on analog in-memory computation of matrix multiplication have recently gained significant attention for reducing the energy of neural network inference. However, analog processing within memory arrays must contend with the issue of parasitic voltage drops across the metal interconnects, which distort the results of the computation and limit the array size. This work analyzes how parasitic resistance affects the end-to-end inference accuracy of state-of-the-art convolutional neural networks, and comprehensively studies how various design decisions at the device, circuit, architecture, and algorithm levels affect the system's sensitivity to parasitic resistance effects. Here, a set of guidelines are provided for how to design analog accelerator hardware that is intrinsically robust to parasitic resistance, without any explicit compensation or re-training of the network parameters.

97 MATHEMATICS AND COMPUTING↗

A PWM Strategy for Cascaded H-bridges to Reduce the Loss Caused by Parasitic Capacitances of Medium Voltage Dual Active Bridge Transformers

In this paper, to reduce the power loss caused by the parasitic capacitances of medium voltage medium frequency transformers in a two-stage dc/ac converter, including a dual active bridge (DAB)-based dc/dc stage and a cascaded H-bridge (CHB)-based dc/ac stage, a PWM strategy is proposed for the dc/ac stage. For each H-bridge, by shifting the high switching frequency to one half-bridge and having the other half-bridge switching at the fundamental frequency of the ac voltage, equivalent frequencies of transformer terminal-to-ground voltages are reduced. As a result, the frequencies of charging and discharging current to the parasitic capacitance are reduced, and therefore the loss caused by the parasitic capacitances is reduced. Compared to the conventional phase-shift PWM, the proposed PWM strategy can reduce the parasitic capacitance loss by about 75%. Experimental test results of one phase in the 13.8 kV/ 100 kW converter prototype are provided to validate the approach.

Li, Haiguo↗

Effect of parasitic plasma currents on solar-array power output

Solar-array voltage-current curves are calculated by assuming the existence of parasitic loads that consist of local currents of charged particles collected by the array. Three cases of interest are calculated to demonstrate how the distribution and magnitude of parasitic currents affect output. Solar array performance degradation became significant when the total parasitic current plus the load current exceeded the short-circuit current. Approximate graphical methods were useful for many applications. Power loss, which was calculated by summing the product of parasitic current and the local potential, underestimated the loss in maximum power.

Domitz, S.↗

Constant current loop impedance measuring system that is immune to the effects of parasitic impedances

A constant current loop measuring system is provided for measuring a characteristic of an environment. The system comprises a first impedance positionable in the environment, a second impedance coupled in series with said first impedance and a parasitic impedance electrically coupled to the first and second impedances. A current generating device, electrically coupled in series with the first and second impedances, provides a constant current through the first and second impedances to produce first and second voltages across the first and second impedances, respectively, and a parasitic voltage across the parasitic impedance. A high impedance voltage measuring device measures a voltage difference between the first and second voltages independent of the parasitic voltage to produce a characteristic voltage representative of the characteristic of the environment.

Anderson, Karl F.↗

Distinct evolutionary lineages of Schistocephalus parasites infecting co-occurring sculpin and stickleback fishes in Alaska

Sculpins (coastrange and slimy) and sticklebacks (ninespine and threespine) are widely distributed fishes cohabiting 2 south-central Alaskan lakes (Aleknagik and Iliamna), and all these species are parasitized by cryptic diphyllobothriidean cestodes in the genus Schistocephalus. The goal of this investigation was to test for host-specific parasitic relationships between sculpins and sticklebacks based upon morphological traits (segment counts) and sequence variation across the NADH1 gene. A total of 446 plerocercoids was examined. Large, significant differences in mean segment counts were found between cestodes in sculpin (mean = 112; standard deviation [S.D.] = 15) and stickleback (mean = 86; S.D. = 9) hosts within and between lakes. Nucleotide sequence divergence between parasites from sculpin and stickleback hosts was 20.5%, and Bayesian phylogenetic analysis recovered 2 well-supported clades of cestodes reflecting intermediate host family (i.e. sculpin, Cottidae vs stickleback, Gasterosteidae). Our findings point to the presence of a distinct lineage of cryptic Schistocephalus in sculpins from Aleknagik and Iliamna lakes that warrants further investigation to determine appropriate evolutionary and taxonomic recognition.

59 BASIC BIOLOGICAL SCIENCES↗

Directed energy system technology for the control of soilborne fungal pathogens and plant‐parasitic nematodes

Abstract BACKGROUND It is challenging to manage soilborne pathogens and plant‐parasitic nematodes using sustainable practices. Here, we evaluated a novel energy application system, Directed Energy System (DES). This system generates pulses of energy capable of impacting selected biological organisms. The oomycete Phytophthora cinnamomi , the fungus Verticillium dahliae , and the plant‐parasitic nematodes Meloidogyne hapla and Globodera ellingtonae were added to soil. Then DES‐generated energy was applied to soil and impacts on target organisms were determined. RESULTS DES applied at 20, 40 and 70 J cm −3 to P. cinnamomi and V. dahliae resulted in ≥50% and 92% reductions (respectively) of propagules per gram of soil in comparison to the untreated control. There was a significant reduction of M. hapla eggs per gram of host tomato root between the untreated control, and 2.2, 13 and 25 J cm −3 DES dosages applied pre‐ or post‐planting. Additionally, an 84% reduction in hatch from G. ellingtonae encysted eggs after treatment with 70 J cm −3 DES was observed. The dosages ranged from 40 or 80V mm −1 for nematodes to 200 V mm −1 for fungi. CONCLUSION DES‐generated energy reduced survival of the soilborne pathogens P. cinnamomi and V. dahlia, and the plant‐parasitic nematodes M. hapla and G. ellingtonae . The application of this technology to a field setting remains to be considered. Published 2020. This article is a U.S. Government work and is in the public domain in the USA. Pest Management Science published by Wiley Periodicals, Inc. on behalf of © 2020 Society of Chemical Industry.

Riga, Ekaterini↗

Parasitic conduction loss of lithium niobate on insulator platform

Thin-film lithium niobate on insulator (LNOI) has recently emerged as an exciting platform for nonlinear integrated photonic devices, drawing parallels with the impact of silicon on insulator (SOI) in the electronic device realm. While the influence of interfacial states of SOI on electronic device performance, particularly their contribution to parasitic radio frequency (RF) losses, is well-documented, this aspect remains relatively unexplored in LNOI, despite their shared silicon/oxide interface structure. Here, we investigate the RF loss arising from mobile charges at the silicon/oxide interface of the LNOI platform. We show that this parasitic conduction can be exacerbated, especially during plasma etching, and leads to substantial RF losses. Through strategic incorporation of a trap-rich poly-silicon layer at this interface, we effectively eliminate this parasitic conduction, resulting in the suppression of RF losses. This work establishes crucial guidelines for harnessing LNOI in applications such as high-frequency electro-mechanical devices, high-speed electro-optic modulators, and high-efficiency microwave-to-optics converters.

Physics↗

Correcting impedance measurements for background parasitics to characterize circuit components in cryogenic environments

Predictable circuit response is a critical prerequisite for accurate electronic measurements. Here, we describe a powerful yet straightforward experimental method and analysis model that utilizes an affordable LCR meter in conjunction with an in situ parasitic-impedance background-correction procedure to measure the temperature-dependent impedance (magnitude and phase) of up to ten individual passive circuit elements in a single cryostat run. We show how the model unambiguously identified a ∼20× drop in capacitance for 22 μF 5XR multilayer ceramic capacitors cooled from 300 K to 360 mK in an environment with a parasitic capacitance of ∼300 pF. The same experimental procedure, based on a simple two-wire measurement, was also used to measure 10 and 22 pF thin-film capacitors and 100 MΩ thick-film resistors. The results showed that the resistor values increased by up to an order of magnitude when the devices were cooled from 300 K to 360 mK. Most importantly, we showed that the simple data-acquisition method, coupled with our analysis model, enabled the measurement of component parasitics, and effectively extended the accuracy of a commercial LCR meter beyond its manufacturer-guaranteed values for a wide range of measurement frequencies. We also showed that, in the current configuration, our simple approach is limited to a capacitance accuracy of ∼10 pF.

Carpenter, R. J. [Santa Clara Univ., Santa Clara, ↗

Boosting the performance of Brillouin amplification at sub-quarter-critical densities via reduction of parasitic Raman scattering

Brillouin amplification of laser pulses in plasma has been shown to be a promising approach to produce picosecond pulses of petawatt power. A key challenge is preservation of the quality of the amplified pulse, which requires control of parasitic instabilities that accompany the amplification process. At high plasma densities (> cr /4), ponderomotive filamentation has been identified as the biggest threat to the integrity of the amplifying pulse. It has therefore been proposed to perform Brillouin scattering at densities below n cr /4 to reduce the influence of filamentation. However, parasitic Raman scattering can become a problem at such densities, contrary to densities above n cr /4 where it is forbidden. In this paper, we investigate the influence of parasitic Raman scattering on Brillouin amplification at densities below n cr /4. We expose the specific problems posed by both Raman backward and forward scattering, and how both types of scattering can be mitigated, leading to an increased performance of the Brillouin amplification process.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Suppression of Parasitic Voltage Oscillation in Power Modules by Turn-Off Current Zero Placement

This paper presents a frequency-domain analysis of the turn-off equivalent switching network of a hard-switched semiconductor switching cell. This paper also proposes a novel method to suppress parasitic voltage ringing by placing the turn-off current zero at the parasitic resonant frequency. The turn-off current was decomposed into ramp functions and a constant-valued function to facilitate obtaining the frequency components of the turn-off current using the Laplace transform of standard signals. The proposed frequency-domain modeling of the parasitic voltage ringing and the suppression were validated by detailed circuit simulation using parameters corresponding to prior literature with experimental validation.

Galigekere, Veda Prakash↗

Array trade-off study using multilayer parasitic subarrays

The use of multilayer parasitic patch arrays in a microstrip phased array offers many potential advantages. An analytical study of microstrip arrays with high gain multilayer parasitic patch subarrays and conventional patch antennas is presented. It is indicated that a thinned array of half as many multilayer parasitic patch subarrays (per row and column) at twice the spacing will perform as well as the full array of ordinary patch antennas. The criterion for comparison was array gain, 3 dB beamwidth and sidelobe level. The attendant reduction in the required number of patch antennas and consequently, MMIC phase shifters is very significant in terms of array complexity, cost and power loss.

Zaman, A.↗

Scan-independent slot arrays with parasitic wire arrays in stratified medium

The effect of a parasitic wire array on the scan admittance of a slot array has been investigated. Structures considered can consist of an infinite slot array and an arbitrary number of parasitic infinte arrays of piecewise linear wires, all arrays being embedded in a stratified medium. These include, as particular cases, phased arrays of Clavin elements. Expressing the fields from the arrays as plane waves, a procedure similar to the periodic moment method for infinite periodic structures is set up to obtain the the scan admittance of the slot array. Scan admittances are presented for a slot array with monopole arrays in free space, and a slot array with a tilted dipole array in a stratified medium. Blind spots at which the incident energy is mainly reflected rather than transmitted were found. Results obtained indicate the possibility of using parasitic wire arrays for scan compensation of active slot arrays.

Ng, Kwong T.↗

Controlled retroreflection - A technique for understanding and eliminating parasitic lasing

Parasitic lasing is examined with an emphasis on understanding and quantifying its effect on high-gain laser systems. Parasitic lasing is unwanted stimulated emission commonly found in high-gain optical systems. A general technique was developed that carefully retroreflects light back into the optical system, thereby creating an externally induced oscillator. Discrepancies between experimental data and threshold calculations for the externally induced oscillator are direct evidence of optical misalignment or of component performance problems. Any changes in the optical system can be directly measured as a change in threshold for the externally induced oscillator. This technique also enables one to align the system for maximum parasitic suppression with the system fully operational. Experimental data illustrating the utility of this technique are presented.

Storm, Mark E.↗

Parasitic instabilities in magnetized, differentially rotating disks

Velikhov, Chandrasekhar, Balbus & Hawley have discovered a MHD instability of differentially rotating fluids that may explain the effective viscosity of accretion disks. If the unperturbed magnetic energy density is much less than the gas pressure, this `magnetorotational' instability (MRI) arises on small scales and is approximately incompressible. If the unperturbed field is suffiently, strong, the MRI is suppressed. We therefore ask whether the MRI mechanism can amplify even very weak initial fields until they reach equipartition with the gas pressure. We show that in the total incompressible limit, the MRI modes are exact solutions of the nonlinear fluid equations, even if the perturbed magnetic field is much larger than the unperturbed field. Also, we present a new exact solution in the opposite limit that the magnetic pressure is much larger than that of the gas. On the other hand, we show that the incompressible MRI modes are themselves subject to parasitic instabilities with instantaneous growth rates proportional to the MRI amplitude. Some of the parasitic instabilities are of the Kelvin-Helmholtz type, while others are less familiar. The eigenfunctions of the latter group suggest that they may promote rapid reconnection of the MRI field. Thus, parasitic modes may stop MRI growth at subequipartition amplitudes if it developes from a sufficiently weak initital field.

Goodman, Jeremy↗

The Effects of Ground Plane and Parasitic Layer on Linearly Tapered Slot Antenna

The effects of a large ground plane and an upper parasitic layer on a linearly tapered slot antenna has been experimentally investigated. Results indicate that the presence of a large ground plane causes the beam to steer by as much as 50 deg from the endfire direction in the H-plane. With the addition of a parasitic layer above the fed antenna, further beam scanning can be achieved when the spacing between the fed and parasitic layers is properly chosen.

Lee, Richard Q.↗

Does Magnetic Field Affect Malaria Parasite Replication in Human Red Blood Cells?

Digestion of red blood cell (RBC) hemoglobin by the malaria parasite results in the formation of paramagnetic hemazoin crystals inside the parasite body. A number of reports suggest that magnetic field interaction with hamazoin crystals significantly reduces the number of infected cells in culture, and thus magnetic field can be used to combat malaria. We studies the effects of magnetic filed on the Plasmodium falciparum asexual life cycle inside RBCs under various experimental conditions. No effect was found during prolonged exposure of infected RBCs to constant magnetic fields up to 6000 Gauss. Infected RBCs were also exposed, under temperature-controlled conditions, to oscillating magnetic fields with frequencies in the range of 500-20000 kHz, and field strength 30-600 Gauss. This exposure often changed the proportion of different parasite stages in treated culture compared to controls. However, no significant effect on parasitemia was observed in treated cultures. This result indicates that the magnetic field effect on Plasmodium falciparum is negligible, or that hypothetical negative and positive effects on different stages within one 48-hour compensate each other.

Chanturiya, Alexandr N.↗

Effect of Parasitic Dielectric Resonators on CPW/Aperture-Coupled Dielectric Resonator Antennas

The effects of parasitic dielectric resonators on the HE (sub 11 sigma) and HE (High Efficiency) (sub 13 sigma) modes of a cylindrical dielectric resonator antenna (DRA) have been studied. The DRA was excited electromagnetically with a grounded coplanar waveguide through an aperture in the common ground plane. Strong couplings were observed for the HE (sub 11 sigma) mode with the parasitic element superimposed on the driven DRA, and for the HE (sub 13 sigma) mode with parasitic elements placed on both sides of the driven DRA. Results indicate significant enhancement in bandwidth for both modes, and good radiation patterns for the HE (sub 11 sigma) mode.

Simons, R. N.↗