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At least 37 records · Page 2

BOREAS TE-6 Biomass and Foliage Area Data

The BOREAS TE-6 team collected several data sets in support of its efforts to characterize and interpret information on the plant biomass, allometry, biometry, sapwood, leaf area index, net primary production, soil temperature, leaf water potential, soil CO, flux, and multivegetation imagery of boreal vegetation. This data set contains measurements of estimates of the standing biomass and leaf area index for the plant species at the TF, CEV, and AUX sites in the SSA and NSA during the growing seasons of 1994 and 1995. The data are stored in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Activity Archive Center (DAAC).

Hall, Forrest G.↗

Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting

Light-emitting diodes (LEDs) are a potential irradiation source for intensive plant culture systems and photobiological research. They have small size, low mass, a long functional life, and narrow spectral output. In this study, we measured the growth and dry matter partitioning of 'Hungarian Wax' pepper (Capsicum annuum L.) plants grown under red LEDs compared with similar plants grown under red LEDs with supplemental blue or far-red radiation or under broad spectrum metal halide (MH) lamps. Additionally, we describe the thermal and spectral characteristics of these sources. The LEDs used in this study had a narrow bandwidth at half peak height (25 nm) and a focused maximum spectral output at 660 nm for the red and 735 nm for the far-red. Near infrared radiation (800 to 3000 nm) was below detection and thermal infrared radiation (3000 to 50,000 nm) was lower in the LEDs compared to the MH source. Although the red to far-red ratio varied considerably, the calculated phytochrome photostationary state (phi) was only slightly different between the radiation sources. Plant biomass was reduced when peppers were grown under red LEDs in the absence of blue wavelengths compared to plants grown under supplemental blue fluorescent lamps or MH lamps. The addition of far-red radiation resulted in taller plants with greater stem mass than red LEDs alone. There were fewer leaves under red or red plus far-red radiation than with lamps producing blue wavelengths. These results indicate that red LEDs may be suitable, in proper combination with other wavelengths of light, for the culture of plants in tightly controlled environments such as space-based plant culture systems.

NASA Discipline Life Support Systems↗

Excess nutrients in hydroponic solutions alter nutrient content of rice, wheat, and potato

Environment has significant effects on the nutrient content of field-grown crop plants. Little is known, however, about compositional changes caused by controlled environments in which plants receive only artificial radiation and soilless, hydroponic culture. This knowledge is essential for developing a safe, nutritious diet in a Controlled Ecological Life-Support System (CELSS). Three crops that are candidates for inclusion in a CELSS (rice, wheat, and white potato) were grown both in the field and in controlled environments where the hydroponic nutrient solution, photosynthetic photon flux (PPF), and CO2 level were manipulated to achieve rapid growth rates. Plants were harvested at maturity, separated into discrete parts, and dried prior to analysis. Plant materials were analyzed for proximate composition (protein, fat, ash, and carbohydrate), total nitrogen (N), nitrate, minerals, and amino-acid composition. The effect of environment on nutrient content varied by crop and plant part. Total N and nonprotein N (NPN) contents of plant biomass generally increased under controlled-environment conditions compared to field conditions, especially for leafy plant parts and roots. Nitrate levels were increased in hydroponically-grown vegetative tissues, but nitrate was excluded from grains and tubers. Mineral content changes in plant tissue included increased phosphorus and decreased levels of certain micronutrient elements under controlled-environment conditions. These findings suggest that cultivar selection, genetic manipulation, and environmental control could be important to obtain highly nutritious biomass in a CELSS.

NASA Discipline Number 61-20↗

How Does Water Delivery System Design Impact the Microbial Load of Salad Crops?

In a microgravity setting, such as the environment aboard the International Space Station (ISS), an ideal plant water delivery system is one that can grow edible crops with minimal resource consumption and minimal risk to crew members. There are also concerns associated with the ability to control fluid escape and biofilm formation resulting in potential dangers to systems, crops, or crewmembers. To identify an appropriate system, candidate systems were assembled and operated under simulated ISS environmental conditions (T,CO2,and RH) with red romaine lettuce (Lactuca sativa cultivar 'Outredgeous') as a model crop. Fluid reservoirs and randomly selected planting sites were sampled every seven days until maturity at which point edible plant biomass and root samples were also taken. Heterotrophic bacteria and fungi growth patterns throughout each planting cycle were determined by plate counts on appropriate agar media. The candidate systems were compared to a classic hydroponics system as a control and harvested crops were compared to controls as well as Veggie-grown and market produce. Plants harvested from candidate systems yielded lower average heterotrophic bacteria and fungi per gram of plant mass levels when compared to market and Veggie samples as well as those from the control system. Additional studies to evaluate the system sanitation regimen as well as testing additional crops should be considered to aid in the selection of an ideal system.

Curry, Aaron B.↗

Potential Carbon Negative Commercial Aviation through Land Management

Brazilian terra preta soil and char-enhanced soil agricultural systems have demonstrated both enhanced plant biomass and crop yield and functions as a carbon sink. Similar carbon sinking has been demonstrated for both glycophyte and halophyte plants and plant roots. Within the assumption of 3.7 t-C/ha/yr soils and plant root carbon sinking, it is possible to provide carbon neutral U.S. commercial aviation using about 8.5% of U.S. arable lands. The total airline CO2 release would be offset by carbon credits for properly managed soils and plant rooting, becoming carbon neutral for carbon sequestered synjet processing. If these lands were also used to produce biomass fuel crops such as soybeans at an increased yield of 60 bu/acre (225gal/ha), they would provide over 3.15 10(exp 9) gallons biodiesel fuel. If all this fuel were refined into biojet it would provide a 16% biojet-84% synjet blend. This allows the U.S. aviation industry to become carbon negative (carbon negative commercial aviation through carbon credits). Arid land recovery could yield even greater benefits.

Hendricks, Robert C.↗

Plasma Assisted Nutrient Recovery from Inedible Biomass via Ash Leaching

Sustaining a human presence on the Moon, Mars, or deep space will require closing loops on many life support systems. Some form of agriculture will be required because plants produce the vitamins, antioxidants, and essential oils in our diets that degrade over time in stored foods. In addition, they provide dietary fiber, restore air, and purify water. Growing plants will require recycling nutrients trapped in inedible vegetation. Researchers at KSC have investigated the use of a thermal plasma with various carrier gases to thermally degrade inedible plant biomass for nutrient recovery. Previous work demonstrated a thermally degrading environment such as a muffle furnace improved nutrient recovery from inedible biomass prior to an acid leaching process. However, a muffle furnace is an inefficient process. We have explored the use of a small scale, thermal plasma for degradation of pellets to enhance the breakdown of plant stems, leaves, and debris to further close the nutrient loop. Plasma carrier gases such as carbon dioxide (CO2), nitrogen (N2), and air were used to explore variations in recovery and potential chemical by-products. Plasma processed inedible biomass was added to varying concentrations of acid solution for leaching of nutrients (e.g. potassium (K) , magnesium (Mg), calcium (Ca), and phosphorus (P)) for reuse in the crop production cycle. We also examined total N2 recovery. Results are presented showing the impact of plasma processing prior to acid leaching on recovery of plant nutrients.

Kenneth Engeling↗

The influence of grazing on surface climatological variables of tallgrass prairie

Mass and energy exchange between most grassland canopies and the atmosphere are mediated by grazing activities. Ambient temperatures can be increased or decreased by grazers. Data have been assembled from simulated grazing experiments on Konza Prairie Research Natural Area and observations on adjacent pastures grazed by cattle show significant changes in primary production, nutrient content, and bidirectional reflectance characteristics as a function of grazing intensity. The purpose of this research was to provide algorithms that would allow incorporation of grazing effects into models of energy budgets using remote sensing procedures. The approach involved: (1) linking empirical measurements of plant biomass and grazing intensities to remotely sensed canopy reflectance, and (2) using a higher resolution, mechanistic grazing model to derive plant ecophysiological parameters that influence reflectance and other surface climatological variables.

Seastedt, T. R.↗

Effects of CO2 on stomatal conductance: do stomata open at very high CO2 concentrations?

Potato and wheat plants were grown for 50 d at 400, 1000 and 10000 micromoles mol-1 carbon dioxide (CO2). and sweetpotato and soybean were grown at 1000 micromoles mol-1 CO2 in controlled environment chambers to study stomatal conductance and plant water use. Lighting was provided with fluorescent lamps as a 12 h photoperiod with 300 micromoles m-2 s-1 PAR. Mid-day stomatal conductances for potato were greatest at 400 and 10000 micromoles mol-1 and least at 1000 micromoles mol-1 CO2. Mid-day conductances for wheat were greatest at 400 micromoles mol-1 and least at 1000 and 10000 micromoles mol-1 CO2. Mid-dark period conductances for potato were significantly greater at 10000 micromoles mol-1 than at 400 or 1000 micromoles mol-1, whereas dark conductance for wheat was similar in all CO2 treatments. Temporarily changing the CO2 concentration from the native 1000 micromoles mol-1 to 400 micromoles mol-1 increased mid-day conductance for all species, while temporarily changing from 1000 to 10000 micromoles mol-1 also increased conductance for potato and sweetpotato. Temporarily changing the dark period CO2 from 1000 to 10000 micromoles mol-1 increased conductance for potato, soybean and sweetpotato. In all cases, the stomatal responses were reversible, i.e. conductances returned to original rates following temporary changes in CO2 concentration. Canopy water use for potato was greatest at 10000, intermediate at 400, and least at 1000 micromoles mol-1 CO2, whereas canopy water use for wheat was greatest at 400 and similar at 1000 and 10000 micromoles mol-1 CO2. Elevated CO2 treatments (i.e. 1000 and 10000 micromoles mol-1) resulted in increased plant biomass for both wheat and potato relative to 400 micromoles mol-1, and no injurious effects were apparent from the 10000 micromoles mol-1 treatment. Results indicate that super-elevated CO2 (i.e. 10000 micromoles mol-1) can increase stomatal conductance in some species, particularly during the dark period, resulting in increased water use and decreased water use efficiency.

NASA Discipline Life Support Systems↗

Control of water and nutrients using a porous tube - A method for growing plants in space

A plant nutrient delivery system that uses a microporous, hydrophilic tube was developed with potential application for crop production in the microgravity of space. The tube contains a nutrient solution and delivers it to the roots. Pumps attached to the tubing create a very small suction that holds the solution within the tube. This system was used to grow wheat for 107 d in a controlled environment at suctions of 0.40, 1.48, or 2.58 kPa. The water absorbed through the pores of the tube by baby diaper sections decreased as suction increased. Correspondingly, final plant biomass, seed number, and spikelet number also tended to decrease as suction increased. The reduced yield at higher suction suggests that the plants experienced water stress, although all suctions were below those typical of soils at field capacity.

Dreschel, Thomas W.↗

Simple radiative transfer model for relationships between canopy biomass and reflectance

A modified Kubelka-Munk model has been utilized to derive useful equations for the analysis of apparent canopy reflectance. Based on the solution to the model simple working equations were formulated by employing reflectance characteristic parameters. The relationships derived show the asymptotic nature of reflectance data that is typically observed in remote sensing studies of plant biomass. They also establish the range of expected apparent canopy reflectance values for specific plant canopy types. The usefulness of the simplified equations was demonstrated by the exceptionally close fit of the theoretical curves to two separately acquired data sets for alfalfa and shortgrass prairie canopies.

Park, J. K.↗

Interaction and Synergism of Microbial Fuel Cell Bacteria within Methanogenesis

Biological hydrogen production from waste biomass has both terrestrial and Martian advanced life support applications. On earth, biological hydrogen production is being explored as a greenhouse neutral form of clean and efficient energy. In a permanently enclosed space habitat, carbon loop closure is required to reduce mission costs. Plants are grown to revitalize oxygen supply and are consumed by habitat inhabitants. Unharvested portions must then be recycled for reuse in the habitat. Several biological degradation techniques exist, but one process, biophotolysis, can be used to produce hydrogen from inedible plant biomass. This process is two-stage, with one stage using dark fermentation to convert plant wastes into organic acids. The second stage, photofermentation, uses photoheterotrophic purple non-sulfur bacteria with the addition of light to turn the organic acids into hydrogen and carbon dioxide. Such a system can prove useful as a co-generation scheme, providing some of the energy needed to power a larger primary carbon recovery system, such as composting. Since butyrate is expected as one of the major inputs into photofermentation, a characterization study was conducted with the bacterium Rhodobacter sphaeroides SCJ, a novel photoheterotrophic non-sulfur purple bacteria, to examine hydrogen production performance at 10 mM-100 mM butyrate concentrations. As butyrate levels increased, hydrogen production increased up to 25 mM, and then decreased and ceased by 100 mM. Additionally, lag phase increased with butyrate concentration, possibly indicating some product inhibition. Maximal substrate conversion efficiency was 8.0%; maximal light efficiency was 0.89%; and maximal hydrogen production rate was 7.7 Umol/mg/cdw/hr (173 ul/mg cdw/hr). These values were either consistent or lower than expected from literature.

Klaus, David↗

BOREAS TF-11 Biomass Data over the SSA-Fen

The BOREAS TF-11 team collected several data sets in its efforts to fully describe the flux and site characteristics at the SSA-Fen site. This data set contains plant cover, standing crop of plant biomass, and estimated net primary productivity at each chamber site at the end of the 1994 field season. The measurements were conducted as part of a 2 x 2 factorial experiment in which we added carbon (300 g/sq m as wheat straw) and nitrogen (6 g/sq m as urea) to four replicate locations in the vicinity of the TF-11 tower. The data are stored in tabular ASCII files.

Valentine, David W.↗

Culturability as an indicator of succession in microbial communities

Successional theory predicts that opportunistic species with high investment of energy in reproduction and wide niche width will be replaced by equilibrium species with relatively higher investment of energy in maintenance and narrower niche width as communities develop. Since the ability to rapidly grow into a detectable colony on nonselective agar medium could be considered as characteristic of opportunistic types of bacteria, the percentage of culturable cells may be an indicator of successional state in microbial communities. The ratios of culturable cells (colony forming units on R2A agar) to total cells (acridine orange direct microscopic counts) and culturable cells to active cells (reduction of 5-cyano-2,3-ditolyl tetrazolium chloride) were measured over time in two types of laboratory microcosms (the rhizosphere of hydroponically grown wheat and aerobic, continuously stirred tank reactors containing plant biomass) to determine the effectiveness of culturabilty as an index of successional state. The culturable cell:total cell ratio in the rhizosphere decreased from approximately 0.25 to less than 0.05 during the first 30-50 days of plant growth, and from 0.65 to 0.14 during the first 7 days of operation of the bioreactor. The culturable cell:active cell ratio followed similar trends, but the values were consistently greater than the culturable cell:total cell ratio, and even exceeded I in early samples. Follow-up studies used a cultivation-independent method, terminal restriction fragment length polymorphisms (TRFLP) from whole community DNA, to assess community structure. The number of TRFLP peaks increased with time, while the number of culturable types did not, indicating that the general decrease in culturability is associated with a shift in community structure. The ratio of respired to assimilated C-14-labeled amino acids increased with the age of rhizosphere communities, supporting the hypothesis that a shift in resource allocation from growth to maintenance occurs with time. Results from this work indicate that the percentage of culturable cells may be a useful method for assessing the successional state of microbial communities.

NASA Center KSC↗

Effects of management practices on reflectance of spring wheat canopies

The effects of available soil moisture, planting date, nitrogen fertilization, and cultivar on reflectance of spring wheat (Triticum aestivum L.) canopies were investigated. Spectral measurements were acquired on eight dates throughout the growing season, along with measurements of crop maturity stage, leaf area index, biomass, plant height, percent soil cover, and soil moisture. Planting date and available soil moisture were the primary agronomic factors which affected reflectance of spring wheat canopies from tillering to maturity. Comparisons of treatments indicated that during the seedling and tillering stages planting date was associated with 36 percent and 85 percent of variation in red and near infrared reflectances, respectively. As the wheat headed and matured, less of the variation in reflectance was associated with planting date and more with available soil moisture. By mid July, soil moisture accounted for 73 percent and 69 percent of the variation in reflectance in red and near infrared bands, respectively. Differences in spectral reflectance among treatments were attributed to changes in leaf area index, biomass, and percent soil cover. Cultivar and N fertilization rate were associated with very little of the variation in the reflectance of these canopies.

Daughtry, C. S. T.↗

Protocol Development for the NASA-JSC Lunar-Mars Life Support Test Project (LMLSTP) Phase 3 Project: A Report on Baseline Studies at KSC for Continuous Salad Production

The Phase 3 Lunar-Mars Life Support Test Project (LMLSTP) was conducted in a 20-foot chamber at Johnson Space Center. The overall objective of the Phase 3 project was to conduct a 90-day regenerative life support system test involving 4 human subjects to demonstrate an integrated biological and physicochemical life support system. A secondary objective of the Phase 3 LMLSTP was to demonstrate the ability to produce salad-type vegetable by integration of a small benchtop growth chamber located within the crew habitat area. This small chamber, commercially manufactured as the Controlled Environment Research Ecosystem (CERES 2010(TM)), functioned as a means to continuously provide fresh lettuce crops for crew members. The CERES 2010(TM) growth chamber utilized hardware components developed for effective plant biomass production in spaceflight applications. These components included: (1) LED lighting; (2) Astroculture(TM) Root Trays; and (3) Zeoponic media. In planning for the LMLSTP Phase 3, a request was put forward for KSC scientists to generate a protocol for successful continuous planting, culturing, and harvesting of the salad-crop, lettuce. By conducting baseline tests with components of the CERES 2010(TM), a protocol was developed.

Goins, G. D.↗

Processing of nutritious, safe and acceptable foods from CELSS candidate crops

A controlled ecological life-support system (CELSS) is required to sustain life for long-duration space missions. The challenge is preparing a wide variety of tasty, familiar, and nutritious foods from CELSS candidate crops under space environmental conditions. Conventional food processing technologies will have to be modified to adapt to the space environment. Extrusion is one of the processes being examined as a means of converting raw plant biomass into familiar foods. A nutrition-improved pasta has been developed using cowpea as a replacement for a portion of the durum semolina. A freeze-drying system that simulates the space conditions has also been developed. Other technologies that would fulfill the requirements of a CELSS will also be addressed.

NASA Discipline Number 93-10↗

NASA's Biomass Production Chamber: a testbed for bioregenerative life support studies

The Biomass Production Chamber (BPC) located at Kennedy Space Center, FL, USA provides a large (20 m2 area, 113 m3 vol.), closed environment for crop growth tests for NASA's Controlled Ecological Life Support System (CELSS) program. Since the summer of 1988, the chamber has operated on a near-continuous basis (over 1200 days) without any major failures (excluding temporary power losses). During this time, five crops of wheat (64-86 days each), three crops of soybean (90 to 97 days), five crops of lettuce (28-30 days), and four crops of potato (90 to 105 days were grown, producing 481 kg of dry plant biomass, 196 kg edible biomass, 540 kg of oxygen, 94,700 kg of condensed water, and fixing 739 kg of carbon dioxide. Results indicate that total biomass yields were close to expected values for the given light input, but edible biomass yields and harvest indices were slightly lower than expected. Stand photosynthesis, respiration, transpiration, and nutrient uptake rates were monitored throughout growth and development of the different crops, along with the build-up of ethylene and other volatile organic compounds in the atmosphere. Data were also gathered on system hardware maintenance and repair, as well as person-hours required for chamber operation. Future tests will include long-term crop production studies, tests in which nutrients from waste treatment systems will be used to grow new crops, and multi-species tests.

NASA Center KSC↗

The Effect of Growth Environment and Salinity on Lipid Production and Composition of Salicornia virginica

Finding a viable and sustainable source of renewable energy is a global task. Biofuels as a renewable energy source can potentially be a viable option for sustaining long-term energy needs. Biodiesel from halophytes shows great promise due to their ability to serve not only as a fuel source, but a food source as well. Halophytes are one of the few biomass plant species that can tolerate a wide range of saline conditions. We investigate the feasibility of using the halophyte, Salicornia virginica as a biofuel source by conducting a series of experiments utilizing various growth and salinity conditions. The goal is to determine if the saline content of Salicornia virginica in our indoor growth vs outdoor growth conditions has an influence on lipid recovery and total biomass composition. We focused on using standard lipid extraction protocols and characterization methods to evaluate twelve Salicornia virginica samples under six saline values ranging from freshwater to seawater and two growth conditions. The overall goal is to develop an optimal lipid extraction protocol for Salicornia virginica and potentially apply this protocol to halophytes in general.

Bomani, Bilal Mark McDowell↗