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AmeriFlux FLUXNET-1F US-Ne3 Mead - rainfed maize-soybean rotation site

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Ne3 Mead - rainfed maize-soybean rotation site. This is the FLUXNET version of the carbon flux data for the site US-Ne3 Mead - rainfed maize-soybean rotation site produced by applying the standard ONEFlux (1F) software. Site Description - The study site is one of three fields (all located within 1.6 km of each other) at the University of Nebraska Agricultural Research and Development Center near Mead, Nebraska. While the other two sites are equipped with irrigation systems, this site relies on rainfall. A tillage operation (disking) was done just prior to the 2001 planting to homogenize the top 0.1 m of soil, incorporate P and K fertilizers, as well as previously accumulated surface residues. Since initiation of the study in 2001, this site has been under a strictly no-till, maize-soybean rotation (maize odd years and soybean even years).

Suyker, Andy [University of Nebraska - Lincoln]

Radar backscatter properties of milo and soybeans

The radar backscatter from fields of milo and soybeans was measured with a ground based radar as a function of frequency (8-18 GHz), polarization (HH and VV) and angle of incidence (0 deg-70 deg) during the summer of 1974. Supporting ground truth was gathered contemporaneously with the backscatter data. At nadir sigma deg of milo correlated highly, r = 0.96, with soil moisture in the milo field at 8.6 GHz but decreased to a value of r = 0.78 at a frequency of 17.0 GHz. Correlation studies of the variations of sigma deg with soil moisture in the soybean fields were not possible due to a lack of a meaningful soil moisture dynamic range. At the larger angles of incidence, however, sigma deg of soybeans did appear to be dependent on precipitation. It is suggested this phenomenon was caused by the rain altering plant geometry. In general sigma deg of both milo and soybeans had a relatively small dynamic range at the higher angles of incidence and showed no significant dependence on the measured crop parameters.

Bush, T. F.

Seasonal soybean crop reflectance

Data are presented from field measurements of 1980 including 5 acquisitions of handheld radiometer reflectance measurements, 7 complete sets of parameters for implementing the Suits mode, and other biophysical parameters to characterize the soybean canopy. LANDSAT calculations on the simulated Brazilian soybean reflectance are included along with data collected during the summer and fall on 1981 on soybean single leaf optical parameters for three irrigation treatments. Tests of the Suits vegetative canopy reflectance model for the full hemisphere of observer directions as well as the nadir direction show moderate agreement for the visible channels of the MSS and poor agreement in the near infrared channel. Temporal changes in the spectral characteristics of the single leaves were seen to occur as a function of maturity which demonstrates that the absorptance of a soybean single leaf is more a function of thetransmittancee characteristics than the seasonally consistent single leaf reflectance.

Lemaster, E. W.

Application of Thematic Mapper data to corn and soybean development stage estimation

A model, utilizing direct relationship between remotely sensed spectral data and the development stage of both corn and soybeans has been proposed and published previously (Badhwar and Henderson, 1981; and Henderson and Badhwar, 1984). This model was developed using data acquired by instruments mounted on trucks over field plots of corn and soybeans as well as satellite data from Landsat. In all cases, the data was analyzed in the spectral bands equivalent to the four bands of Landsat multispectral scanner (MSS). In this study the same model has been applied to corn and soybeans using Landsat-4 Thematic Mapper (TM) data combined with simulated TM data to provide a multitemporal data set in TM band intervals. All data (five total acquisitions) were acquired over a test site in Webster County, Iowa from June to October 1982. The use of TM data for determining development state is as accurate as with Landsat MSS and field plot data in MSS bands. The maximum deviation of 0.6 development stage for corn and 0.8 development stage for soybeans is well within the uncertainty with which a field can be estimated with procedures used by observers on the ground in 1982.

Badhwar, G. D.

Growth in microgravity increases susceptibility of soybean to a fungal pathogen

The influence of microgravity on the susceptibility of soybean roots to Phytophthora sojae was studied during the Space Shuttle Mission STS-87. Seedlings of soybean cultivar Williams 82 grown in spaceflight or at unit gravity were untreated or inoculated with the soybean root rot pathogen P. sojae. At 3, 6 and 7 d after launch while still in microgravity, seedlings were photographed and then fixed for subsequent microscopic analysis. Post-landing analysis of the seedlings revealed that at harvest day 7 the length of untreated roots did not differ between flight and ground samples. However, the flight-grown roots infected with P. sojae showed more disease symptoms (percentage of brown and macerated areas) and the root tissues were more extensively colonized relative to the ground controls exposed to the fungus. Ethylene levels were higher in spaceflight when compared to ground samples. These data suggest that soybean seedlings grown in microgravity are more susceptible to colonization by a fungal pathogen relative to ground controls.

manned

Differences in the response of wheat, soybean and lettuce to reduced blue radiation

Although many fundamental blue light responses have been identified, blue light dose-response curves are not well characterized. We studied the growth and development of soybean, wheat and lettuce plants under high-pressure sodium (HPS) and metal halide (MH) lamps with yellow filters creating five fractions of blue light. The blue light fractions obtained were < 0.1, 2 and 6% under HPS lamps, and 6, 12 and 26% under MH lamps. Studies utilizing both lamp types were done at two photosynthetic photon flux levels, 200 and 500 mumol m-2 s-1 under a 16 h photoperiod. Phytochrome photoequilibria was nearly identical among treatments. The blue light effect on dry mass, stem length, leaf area, specific leaf area and tillering/branching was species dependent. For these parameters, wheat did not respond to blue light, but lettuce was highly sensitive to blue light fraction between 0 and 6% blue. Soybean stem length decreased and leaf area increased up to 6% blue, but total dry mass was unchanged. The blue light fraction determined the stem elongation response in soybean, whereas the absolute amount of blue light determined the stem elongation response in lettuce. The data indicate that lettuce growth and development requires blue light, but soybean and wheat may not.

NASA Discipline Life Support Systems

Long-term Blue Light Effects on the Histology of Lettuce and Soybean Leaves and Stems

Blue light (320 to 496 nm) alters hypocotyl and stem elongation and leaf expansion in short-term, cell-level experiments, but histological effects of blue light in long-term studies of whole plants have not been described. We measured cell size and number in stems of soybean (Glycine max L.) and leaves of soybean and lettuce (Lactuca sativa L.), at two blue light fractions. Short-term studies have shown that cell expansion in stems is rapidly inhibited when etiolated tissue is exposed to blue light. However, under long-term light exposure, an increase in the blue light fraction from less than 0.1% to 26% decreased internode length, specifically by inhibiting soybean cell division in stems. In contrast, an increase in blue light fraction from 6% to 26% reduced soybean leaf area by decreasing cell expansion. Surprisingly, lettuce leaf area increased with increasing blue light fraction (0% to 6%), which was attributed to a 3.1-fold increase in cell expansion and a 1.6-fold increase in cell division.

Dougher, Tracy A. O.

Assembly, comparative analysis, and utilization of a single haplotype reference genome for soybean

Cultivar Williams 82 has served as the reference genome for the soybean research community since 2008, but is known to have areas of genomic heterogeneity among different sub-lines. This work provides an updated assembly (version Wm82.a6) derived from a specific sub-line known as Wm82-ISU-01 (seeds available under USDA accession PI 704477). The genome was assembled using Pacific BioSciences HiFi reads and integrated into chromosomes using HiC. The 20 soybean chromosomes assembled into a genome of 1.01Gb, consisting of 36 contigs. The genome annotation identified 48 387 gene models, named in accordance with previous assembly versions Wm82.a2 and Wm82.a4. Comparisons of Wm82.a6 with other near-gapless assemblies of Williams 82 reveal large regions of genomic heterogeneity, including regions of differential introgression from the cultivar Kingwa within approximately 30 Mb and 25 Mb segments on chromosomes 03 and 07, respectively. Additionally, our analysis revealed a previously unknown large (> 20 Mb) heterogeneous region in the pericentromeric region of chromosome 12, where Wm82.a6 matches the ‘Williams’ haplotype while the other two near-gapless assemblies do not match the haplotype of either parent of Williams 82. In addition to the Wm82.a6 assembly, we also assembled the genome of ‘Fiskeby III,’ a rich resource for abiotic stress resistance genes. A genome comparison of Wm82.a6 with Fiskeby III revealed the nucleotide and structural polymorphisms between the two genomes within a QTL region for iron deficiency chlorosis resistance. The Wm82.a6 and Fiskeby III genomes described here will enhance comparative and functional genomics capacities and applications in the soybean community.

59 BASIC BIOLOGICAL SCIENCES

AmeriFlux FLUXNET-1F US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage. This is the FLUXNET version of the carbon flux data for the site US-MN1 Morris: Corn-Soybean with Cover Crops, Strip Tillage produced by applying the standard ONEFlux (1F) software. Site Description - Cropland, 2-year rotation (corn-soybean), strip tillage with winter cover crops (annual rye), nutrient management by soil tests, uniform pest management, tile drainage

Helseth, Christina [USDA-ARS LTAR]

AmeriFlux FLUXNET-1F US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage. This is the FLUXNET version of the carbon flux data for the site US-MN2 Morris: Corn-Soybean-Wheat with Cover Crops, Minimal Tillage produced by applying the standard ONEFlux (1F) software. Site Description - Cropland, 3-year rotation (corn-soybean-wheat), cover crops (winter camelina and pennycress), minimum tillage, nutrient management by soil tests, uniform pest management, tile drainage

Helseth, Christina [USDA-ARS LTAR]

AmeriFlux FLUXNET-1F US-MN3 Morris: Corn-Soybean, Conventional Tillage

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-MN3 Morris: Corn-Soybean, Conventional Tillage. This is the FLUXNET version of the carbon flux data for the site US-MN3 Morris: Corn-Soybean, Conventional Tillage produced by applying the standard ONEFlux (1F) software. Site Description - Cropland, 2-year rotation (corn-soybean), conventional (deep rip) tillage, uniform nutrient management, uniform pest management, tile drainage

Helseth, Christina [USDA-ARS LTAR]

Crop identification studies using Landsat data Separation of barley from other spring small grains and corn and soybean decision logic

Two labeling procedures were developed which identify various agricultural crops through the use of Landsat data. One procedure separates barley from other spring small grains, and the other identifies corn and soybeans. For both procedures, a minimum data set (critical acquisition time) has been designated. Landsat data in both image format and various graphic displays were used along with ancillary data to obtain information which aided in labeling the spectral signatures. The corn and soybean procedure also employed a structured decision logic. Test results for the barley separation procedure emphasized the importance of obtaining a critical acquisition and showed some success especially in areas where spring crops followed the expected growth patterns. Two tests of the corn and soybean procedure produced good labeling accuracies. Problems with the procedure were easy to identify, and some solutions were implemented for the second test. Automation of various parts of the procedure and extension to other crops and regions were recommended.

Dailey, C. L.

AgRISTARS: Foreign commodity production forecasting. The 1980 US corn and soybeans exploratory experiment

The U.S. corn and soybeans exploratory experiment is described which consisted of evaluations of two technology components of a production forecasting system: classification procedures (crop labeling and proportion estimation at the level of a sampling unit) and sampling and aggregation procedures. The results from the labeling evaluations indicate that the corn and soybeans labeling procedure works very well in the U.S. corn belt with full season (after tasseling) LANDSAT data. The procedure should be readily adaptable to corn and soybeans labeling required for subsequent exploratory experiments or pilot tests. The machine classification procedures evaluated in this experiment were not effective in improving the proportion estimates. The corn proportions produced by the machine procedures had a large bias when the bias correction was not performed. This bias was caused by the manner in which the machine procedures handled spectrally impure pixels. The simulation test indicated that the weighted aggregation procedure performed quite well. Although further work can be done to improve both the simulation tests and the aggregation procedure, the results of this test show that the procedure should serve as a useful baseline procedure in future exploratory experiments and pilot tests.

Malin, J. T.

Fiscal year 1981 US corn and soybeans pilot preliminary experiment plan, phase 1

A draft of the preliminary experiment plan for the foreign commodity production forecasting project fiscal year 1981 is presented. This draft plan includes: definition of the phase 1 and 2 U.S. pilot objectives; the proposed experiment design to evaluate crop calendar, area estimation, and area aggregation components for corn and soybean technologies using 1978/1979 crop-year data; a description of individual sensitivity evaluations of the baseline corn and soybean segment classification procedure; and technology and data assessment in support of the corn and soybean estimation technology for use in the U.S. central corn belt.

Livingston, G. P.

Soybean canopy reflectance as a function of view and illumination geometry

The results of an experiment designed to characterize a soybean field by its reflectance at various view and illumination angles and by its physical and agronomic attributes are presented. Reflectances were calculated from measurements at four wavelength bands through eight view azimuth and seven view zenith directions for various solar zenith and azimuth angles during portions of three days. An ancillary data set consisting of the agronomic and physical characteristics of the soybean field is described. The results indicate that the distribution of reflectance from a soybean field is a function of the solar illumination and viewing geometry, wavelength and row direction, as well as the state of development of the canopy. Shadows between rows greatly affected the reflectance in the visible wavelength bands and to a lesser extent in the near infrared wavelengths. A model is proposed that describes the reflectance variation as a function of projected solar and projected viewing angles. The model appears to approximate the reflectance variations in the visible wavelength bands from a canopy with well defined row structure.

Bauer, M. E.

Analysis of the profile characteristics of corn and soybeans using field reflectance data

The typical patterns of spectral development (profiles) for corn and soybeans are presented, based on field-collected reflectance data transformed to correspond to LANDSAT-MSS Tasseled Cap coordinates. Reasonable variations in field conditions and cultural practices are shown to significantly influence profile features. The separability of the two crops is determined to be primarily related to the maximum value of the reflectance equivalent of Greenness, and to the plateau effect seen in corn Greenness profiles. The impact of changes in conditions on separability is described. In addition, association is made between profile features and stages of development for corn and soybeans. Corn is shown to peak at a stage well before tasseling or maximum LAI, while the characteristics of the soybean profile are shown to be unrelated to any particular stage of development.

Crist, E. P.