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NASA NTRS2004

Two-Photon Fluorescence Microscopy Developed for Microgravity Fluid Physics

Recent research efforts within the Microgravity Fluid Physics Branch of the NASA Glenn Research Center have necessitated the development of a microscope capable of high-resolution, three-dimensional imaging of intracellular structure and tissue morphology. Standard optical microscopy works well for thin samples, but it does not allow the imaging of thick samples because of severe degradation caused by out-of-focus object structure. Confocal microscopy, which is a laser-based scanning microscopy, provides improved three-dimensional imaging and true optical sectioning by excluding the out-of-focus light. However, in confocal microscopy, out-of-focus object structure is still illuminated by the incoming beam, which can lead to substantial photo-bleaching. In addition, confocal microscopy is plagued by limited penetration depth, signal loss due to the presence of a confocal pinhole, and the possibility of live-cell damage. Two-photon microscopy is a novel form of laser-based scanning microscopy that allows three-dimensional imaging without many of the problems inherent in confocal microscopy. Unlike one-photon microscopy, it utilizes the nonlinear absorption of two near-infrared photons. However, the efficiency of two-photon absorption is much lower than that of one-photon absorption because of the nonlinear (i.e., quadratic) electric field dependence, so an ultrafast pulsed laser source must typically be employed. On the other hand, this stringent energy density requirement effectively localizes fluorophore excitation to the focal volume. Consequently, two-photon microscopy provides optical sectioning and confocal performance without the need for a signal-limiting pinhole. In addition, there is a reduction in photo-damage because of the longer excitation wavelength, a reduction in background fluorescence, and a 4 increase in penetration depth over confocal methods because of the reduction in Rayleigh scattering.

Fischer, David G.↗

NASA NTRS2011

Multiple-Event, Single-Photon Counting Imaging Sensor

The single-photon counting imaging sensor is typically an array of silicon Geiger-mode avalanche photodiodes that are monolithically integrated with CMOS (complementary metal oxide semiconductor) readout, signal processing, and addressing circuits located in each pixel and the peripheral area of the chip. The major problem is its single-event method for photon count number registration. A single-event single-photon counting imaging array only allows registration of up to one photon count in each of its pixels during a frame time, i.e., the interval between two successive pixel reset operations. Since the frame time can t be too short, this will lead to very low dynamic range and make the sensor merely useful for very low flux environments. The second problem of the prior technique is a limited fill factor resulting from consumption of chip area by the monolithically integrated CMOS readout in pixels. The resulting low photon collection efficiency will substantially ruin any benefit gained from the very sensitive single-photon counting detection. The single-photon counting imaging sensor developed in this work has a novel multiple-event architecture, which allows each of its pixels to register as more than one million (or more) photon-counting events during a frame time. Because of a consequently boosted dynamic range, the imaging array of the invention is capable of performing single-photon counting under ultra-low light through high-flux environments. On the other hand, since the multiple-event architecture is implemented in a hybrid structure, back-illumination and close-to-unity fill factor can be realized, and maximized quantum efficiency can also be achieved in the detector array.

Zheng, Xinyu↗

NASA NTRS1994

Emission lines from X-ray-heated accretion disks in low-mass X-ray binaries

We investigate the structure of accretion disks illuminated by X-rays from a central compact object in a binary system. X-rays can photoionize the upper atmosphere of the disk and form an accretion disk corona (ADC) where emission lines can form. We construct a model to calculate the vertical structure and the emission spectrum of the ADC with parameters appropriate to low-mass X-ray binaries. These models are made by nonlocal thermodynamic equilibrium calculations of ion and level populations and include a large number of atomic processes for 10 cosmically abundant elements. Transfer of radiation is treated by using the escape probability formalism. The vertical temperature profile of the ADC consists of a Compton-heated region and a mid-T zone where the temperature is approximately 10(exp 6) K. A thermal instability occurs close to the disk photosphere and causes the temperature of the ADC to drop abruptly from 10(exp 6) K to several times 10(exp 4) K. The emission spectrum in the optical, ultraviolet, extreme ultraviolet, and X-ray range is discussed and compared with the observations.

Ko, Yuan-Kuen↗

NASA NTRS1992

Simultaneous mapping of the unsteady flow fields by Particle Displacement Velocimetry (PDV)

Current experimental and computational techniques must be improved in order to advance the prediction capability of the longitudinal vortical flows shed by underwater vehicles. The generation, development, and breakdown mechanisms of the shed vortices at high Reynolds numbers are not fully understood. The ability to measure hull separated vortices associated with vehicle maneuvering does not exist at present. The existing point-by-point measurement techniques can only capture approximately the large 'mean' eddies but fail to meet the dynamics of small vortices during the initial stage of generation. A new technique, which offers a previously unavailable capability to measure the unsteady cross-flow distribution in the plane of the laser light sheet, is called Particle Displacement Velocimetry (PDV). PDV consists of illuminating a thin section of the flowfield with a pulsed laser. The water is seeded with microscopic, neutrally buoyant particles containing imbedded fluorescing dye which responds with intense spontaneous fluorescence with the illuminated section. The seeded particles in the vortical flow structure shed by the underwater vehicle are illuminated by the pulse laser and the corresponding particle traces are recorded in a single photographic frame. Two distinct approaches were utilized for determining the velocity distribution from the particle traces. The first method is based on matching the traces of the same particle and measuring the distance between them. The direction of the flow can be identified by keeping one of the pulses longer than the other. The second method is based on selecting a small window within the image and finding the mean shift of all the particles within that region. The computation of the auto-correlation of the intensity distribution within the selected sample window is used to determine the mean displacement of particles. The direction of the flow is identified by varying the intensity of the laser light between pulses. Considerable computational resources are required to compute the auto-correction of the intensity distribution. Parallel processing will be employed to speed up the data reduction. A few examples of measured unsteady vortical flow structures shed by the underwater vehicles will be presented.

Huang, Thomas T.↗

NASA NTRS1980

Scattering defects in silver mirror coatings

Scatter centers common to protected silver have been investigated on a variety of space flight quality mirrors. The defects have been characterized as to number density, size, location within the film layer structure, and dependence on illumination angle. In addition the total integrated scatter values as a function of wavelength, humidity test soak time, and temperature have been determined for some mirrors. These submicrometer sized defects are believed to be the precursors of silver corrosion spots. A mechanism for their generation is proposed, and measures to reduce their occurrence are suggested.

Pellicori, S. F.↗

NASA NTRS1984

Rheoceptive mediators of graviperception in a water flea: Morphological implications of antennal-socket setae in daphnia magna

Aquatic microcrustaceans of the genus Daphnia are known to orient to light during the day. At night, in the absence of visual cues, daphnids were suspected of maintaining equilibrium by monitoring the direction of gravity through their swimming antennae. Recent investigations using simulated, weightlessness conditions coupled with absence of illumination revealed hair like structures or setae on the basal, articulating socket of the antennae that, when surgically removed, resulted in disorientation. Given the simulated weightlessness or neutrally buoyant condition that eliminated sinking of the normally negatively buoyant Daphnia, it was proposed that the antennal socket setae function as rheoceptors stimulated by the upward rush of water currents during gravity induced, sinking phase of daphnid swimming movements. This rheoceptively mediated, gravity perception hypothesis is further supported by morphological investigations. Scanning electron micrographs indicate that antennal socket setae are anatomically similar to proprioceptors used by higher crustaceans to monitor gravitational direction.

Meyers, D. G.↗

NASA NTRS2009

X-ray Reflected Spectra from Accretion Disk Models. I. Constant Density Atmospheres

We present new models for illuminated accretion disks, their structure and reprocessed emission. We consider the effects of incident X-rays on the surface of an accretion disk by solving simultaneously the equations of radiative transfer, energy balance and ionization equilibrium over a large range of column densities. We assume plane-parallel geometry and azimuthal symmetry, such that each calculation corresponds to a ring at a given distance from the central object. Our models include recent and complete atomic data for K-shell of the iron and oxygen isonuclear sequences. We examine the effect on the spectrum of fluorescent Ka line emission and absorption in the emitted spectrum. We also explore the dependence of the spectrum on the strength of the incident X-rays and other input parameters, and discuss the importance of Comptonization on the emitted spectrum.

Garcia, Javier↗

NASA NTRS2004

Feature-Identification and Data-Compression Software

A report discusses the continuing development of Windows Interface for Nominal Displacement Selection (WINDS), a computer program for automated analysis of images of the Sun and planets acquired by scientific instruments aboard spacecraft. WINDS is intended to afford capabilities for identification of features, measurement of displacements and velocities, analysis of terrain and of atmospheres, and synthesis of animation sequences of images of terrains and atmospheres from small sets of samples by use of velocity-based interpolation. A major element of WINDS will be a nonlinear correlator capable of tracking small features in complex image sequences. For dynamic image sequences, the correlator will enable compression of data by factors >100. In processing image data, WINDS will take account of such factors as texture in image data, rotation of features during measurement intervals, effects of viewing and solar illumination angles, and vertical structures of atmospheres. WINDS will also take account of positions, aiming directions, and fields of view of cameras to determine three-dimensional feature structures by use of triangulation and stereoscopic analysis techniques.

Jong, Eric De↗

NASA NTRS2023

Discovery of X-Ray Polarization Angle Rotation in the Jet From Blazar Mrk 421

The magnetic-field conditions in astrophysical relativistic jets can be probed by multiwavelength polarimetry, which has been recently extended to X-rays. For example, one can track how the magnetic field changes in the flow of the radiating particles by observing rotations of the electric vector position angle Ψ. Here we report the discovery of a ΨX rotation in the X-ray band in the blazar Markarian 421 at an average flux state. Across the 5 days of Imaging X-ray Polarimetry Explorer observations on 4–6 and 7–9 June 2022, ΨX rotated in total by ≥360°. Over the two respective date ranges, we find constant, within uncertainties, rotation rates (80 ± 9° per day and 91 ± 8° per day) and polarization degrees (ΠX = 10% ± 1%). Simulations of a random walk of the polarization vector indicate that it is unlikely that such rotation(s) are produced by a stochastic process. The X-ray-emitting site does not completely overlap the radio, infrared and optical emission sites, as no similar rotation of Ψ was observed in quasi-simultaneous data at longer wavelengths. We propose that the observed rotation was caused by a helical magnetic structure in the jet, illuminated in the X-rays by a localized shock propagating along this helix. The optically emitting region probably lies in a sheath surrounding an inner spine where the X-ray radiation is released.

Makarian 421↗

NASA NTRS2018

System and Method for Active Multispectral Imaging and Optical Communications

Provided is a system and method for active multispectral imaging having a transmitter that uses narrowband optical radiation to dynamically illuminate an object with modulated structured light in multiple spectral bands, and a receiver that includes an independent panchromatic imager. The transmitter and receiver can be operated in a bistatic decoupled configuration to enable passive multispectral synthesis, illumination-invariant sensing, optical communications, and the ability for the transmitter to emit a sequence of spectral bands in an order that is unknown to the receiver, and the receiver is able to passively decode the spectral identity from a band identifier embedded in the modulated structured light. The receiver passively decodes embedded high-bandwidth simplex communications while reconstructing calibrated multispectral images at video frame rates.

Chirayath, Ved↗

NASA NTRS2019

Next-Generation Optical Sensing Technologies for Exploring Ocean Worlds - NASA FluidCam, MiDAR, and NeMO-Net

We highlight three emerging NASA optical technologies that enhance our ability to remotely sense, analyze, and explore ocean worlds–FluidCam and fluid lensing, MiDAR, and NeMO-Net. Fluid lensing is the first remote sensing technology capable of imaging through ocean waves without distortions in 3D at sub-cm resolutions. Fluid lensing and the purpose-built FluidCam CubeSat instruments have been used to provide refraction-corrected 3D multispectral imagery of shallow marine systems from unmanned aerial vehicles (UAVs). Results from repeat 2013 and 2016 airborne fluid lensing campaigns over coral reefs in American Samoa present a promising new tool for monitoring fine-scale ecological dynamics in shallow aquatic systems tens of square kilometers in area. MiDAR is a recently-patented active multispectral remote sensing and optical communications instrument which evolved from FluidCam. MiDAR is being tested on UAVs and autonomous underwater vehicles (AUVs) to remotely sense living and non-living structures in light-limited and analog planetary science environments. MiDAR illuminates targets with high-intensity narrowband structured optical radiation to measure an object’s spectral reflectance while simultaneously transmitting data. MiDAR is capable of remotely sensing reflectance at fine spatial and temporal scales, with a signal-to-noise ratio 10-10(exp 3) times higher than passive airborne and spaceborne remote sensing systems, enabling high-framerate multispectral sensing across the ultraviolet, visible, and near-infrared spectrum. Preliminary results from a 2018 mission to Guam show encouraging applications of MiDAR to imaging coral from airborne and underwater platforms whilst transmitting data across the air-water interface. Finally, we share NeMO-Net, the Neural Multi-Modal Observation & Training Network for Global Coral Reef Assessment. NeMO-Net is a machine learning technology under development that exploits high-resolution data from FluidCam and MiDAR for augmentation of low-resolution airborne and satellite remote sensing. NeMO-Net is intended to harmonize the growing diversity of 2D and 3D remote sensing with in situ data into a single open-source platform for assessing shallow marine ecosystems globally using active learning for citizen-science based training. Preliminary results from four-class Q17 coral classification have an accuracy of 94.4%. Together, these maturing technologies present promising scalable, practical, and cost-efficient innovations that address current observational and technological challenges in optical sensing of marine systems.

Ved Chirayath↗

NASA NTRS2010

Photogrammetric system and method used in the characterization of a structure

A photogrammetric system uses an array of spaced-apart targets coupled to a structure. Each target exhibits fluorescence when exposed to a broad beam of illumination. A photogrammetric imaging system located remotely with respect to the structure detects and processes the fluorescence (but not the illumination wavelength) to measure the shape of a structure.

Dorrington, Adrian A.↗

NASA NTRS1977

MIS diode structure in As/+/ implanted CdS

Structure made by As implantation of carefully prepared high-conductivity CdS surfaces followed by Pt deposition and 450 C anneal display rectifying, although substantially different, I-V characteristics in the dark and during illumination with subband-gap light. Structures prepared in the same way on an unimplanted portion of the substrate have similar I-V characteristics, except that the forward turnover voltage for an illuminated unimplanted diode is much smaller than that for an implanted diode. It is suggested that the charge conduction in both structures is dominated by hole and/or electron tunneling through a metal-semiconductor potential barrier. The tunneling processes appear to be quite sensitive to subband-gap illumination, which causes the dramatic decreases of turnover voltages and apparent series resistances. The difference in turnover voltage appears to be caused by interface states between the Pt electrode and the implanted layer, which suggests a MIS model.

Hutchby, J. A.↗

NASA NTRS1978

Determination of the structure and heating mechanisms of coronal loops from soft X-ray observations with the solar probe

High resolution soft X-ray imaging from the solar probe is justified in terms of the expected scientific returns which include the determination of the temperature and density structure of a coronal loop. The advantages of the grazing incidence telescope over the multiple pinhole camera are discussed. An instrument package is described which includes a grazing incidence mirror, a thermal prefilter, a three position filter wheel and a focal plane detector baselined as an 800 by 800 back-illuminated charge coupled device. The structural assembly together with the data processing equipment would draw heavily on the designs being developed for the Solar Polar Mission.

Davis, J. M.↗

NASA NTRS2012

Computational Ghost Imaging for Remote Sensing

This work relates to the generic problem of remote active imaging; that is, a source illuminates a target of interest and a receiver collects the scattered light off the target to obtain an image. Conventional imaging systems consist of an imaging lens and a high-resolution detector array [e.g., a CCD (charge coupled device) array] to register the image. However, conventional imaging systems for remote sensing require high-quality optics and need to support large detector arrays and associated electronics. This results in suboptimal size, weight, and power consumption. Computational ghost imaging (CGI) is a computational alternative to this traditional imaging concept that has a very simple receiver structure. In CGI, the transmitter illuminates the target with a modulated light source. A single-pixel (bucket) detector collects the scattered light. Then, via computation (i.e., postprocessing), the receiver can reconstruct the image using the knowledge of the modulation that was projected onto the target by the transmitter. This way, one can construct a very simple receiver that, in principle, requires no lens to image a target. Ghost imaging is a transverse imaging modality that has been receiving much attention owing to a rich interconnection of novel physical characteristics and novel signal processing algorithms suitable for active computational imaging. The original ghost imaging experiments consisted of two correlated optical beams traversing distinct paths and impinging on two spatially-separated photodetectors: one beam interacts with the target and then illuminates on a single-pixel (bucket) detector that provides no spatial resolution, whereas the other beam traverses an independent path and impinges on a high-resolution camera without any interaction with the target. The term ghost imaging was coined soon after the initial experiments were reported, to emphasize the fact that by cross-correlating two photocurrents, one generates an image of the target. In CGI, the measurement obtained from the reference arm (with the high-resolution detector) is replaced by a computational derivation of the measurement-plane intensity profile of the reference-arm beam. The algorithms applied to computational ghost imaging have diversified beyond simple correlation measurements, and now include modern reconstruction algorithms based on compressive sensing.

Erkmen, Baris I.↗

NASA NTRS1989

Illumination requirements for orbital SAR - Experience from SIR-B Canadian Shield experiments

Synthetic aperture radar illumination requirements for accurate rendition of geological structure in low-relief regions of uniform radar roughness are considered, along with the effect of illumination azimuth biasing. Results of two Shuttle imaging radar Canadian Shield experiments are discussed with emphasis on look azimuth, look direction, and look angle. It is found out that at least two look azimuths, preferably orthogonal, are essential for adequate rendition of regional structure of the area, while four nearly-orthogonal looks are necessary to compensate for slope orientation. This can be accomplished by orbital design with crossing ground tracks or utilizing a squint mode. Attention is given to the acquisition of coverage from look directions 180 deg apart and to multiple look angles desirable for geological applications.

Lowman, P. D.↗

NASA NTRS1979

Rough surface wavelength measurement through self mixing of Doppler microwave backscatter

A microwave backscatter technique is presented that has the ability to sense the dominant surface wavelength of a random rough surface. The purpose of this technique is to perform this measurement from an aircraft or spacecraft, wherein the horizontal velocity of the radar is an important parameter of the measurement system. Attention will be directed at water surface conditions for which a dominant wavelength can be defined, then the spatial variations of reflectivity will have a two dimensional spectrum that is sufficiently close to that of waves to be useful. The measurement concept is based on the relative motion between the water waves and a nadir looking radar, and the fact that while the instantaneous Doppler frequency at the receiver returned by any elementary group of scatterers on a water wave is monotonically changing, the difference in the Doppler frequency between any two scattering 'patches' stays approximately constant as these waves travel parallel to the major axis of an elliptical antenna footprint. The results of a theoretical analysis and a laboratory experiment with a continuous wave (CW) radar that encompasses several of the largest waves in the illuminated area show how the structure in the Doppler spectrum of the backscattered signal is related to the surface spectrum and its parameters in an especially direct and simple way when an incoherent envelope detector is the receiver.

Weissman, D. E.↗

NASA NTRS1981

Stratospheric ozone response to a solar proton event - Hemispheric asymmetries

Large-scale stratospheric ozone reduction due to precipitating energetic solar protons is examined, based on the backscattered ultraviolet experiment on the Nimbus 4 satellite during the 1972 solar flare. Some chemical equations for the processes are outlined. Distinct asymmetries in the columnar ozone content, the amount of ozone depression, and their temporal variation above 4 mb level (about 38 km) were observed between the two hemispheres. These asymmetries are ascribed to the differences mainly in dynamics and partly in the solar illumination and the vertical temperature structure between the summer and the winter polar atmospheres.

Maeda, K.↗