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At least 37 records · Page 2

Simulation Evaluations of an Autonomous Urban Air Mobility Network Management and Separation Service

This paper presents an initial implementation of an autonomous Urban Air Mobility network management and aircraft separation service for urban airspace that does 1) departure and arrival scheduling across the network, 2) continuous trajectory management to ensure safe separation between aircraft, and 3) seamless integration with traditional operations. The highly-autonomous AutoResolver algorithm developed for traditional aviation was extended to provide these capabilities. An evaluation of this initial implementation was conducted in fast-time simulations using a dense, two-hour traffic scenario with Urban Air Mobility aircraft flying between a network of 20 vertiports in the Dallas-Fort Worth metroplex. When the spatial separation was reduced from 0:3nmi to 0:1nmi, the total de- lay decreased by 7:3%; when the temporal separation was reduced from 60s to 45s, the total delay decreased by 28:4%. The total number of conflict resolutions decreased by 26% and 17%, respectively. Furthermore, when a scheduling horizon greater than the duration of UAM flights was used (50min), most conflicts were resolved pre-departure producing ground delay. By comparison, when a shorter scheduling horizon was used (8min), most conflicts were resolved post-departure generating airborne delay. For all scheduling and separation constraints tested, AutoResolver prevented loss of separation from occurring. Urban Air Mobility operations have the ability to revolutionize how people and goods are transported and this paper presents initial research focusing on the high levels of autonomy required for an airspace system capable of scaling to handle significantly higher densities of aircraft.

Bosson, Christabelle S.↗

Fiber optic sensors with internal referencing

The main problem with amplitude modulating type sensors is that any variation in the intensity of the optical signal which occurs throughout the sensing system is interpreted by the photodetector as resulting from the sensor itself and is reflected as an error in the sensed parameter. To account for these errors, a referencing technique with the signal and reference channels separated in the time domain over the same fiber link can be used. Selected sensing and signal processing techniques involving temporally separated signal and referencing channels are described. A transition from the time into the frequency domain is also discussed. Experimental data are presented.

Adamovsky, Grigory↗

Observation guidelines for a Total Ozone Mapping Spectrometer (TOMS) in geosynchronous orbit

The successful utilization of Total Ozone Mapping Spectrometer (TOMS) measurements in low Earth orbit for the analysis of rapidly changing events has led to the consideration of a TOMS in geosynchronous orbit. This orbit should allow for the selection of temporal and spatial resolutions that are specifically designed for these events, plus the flexibility of selecting different sized areas and pointing the sensor to focus on the most interesting events. Separate temporal and spatial resolution guidelines plus recommended areal coverage have been developed for tropical cyclones, jet streams, the interaction between strong convection and the environment, and the surveillance of volcanoes. It is also suggested that the most effective use of TOMS would be simultaneous flights with microwave and high spatial resolution infrared temperature profiles.

Shenk, William E.↗

Svalbard: A Window for Understanding Temporal/Spatial Aspects of Solar Wind Coupling to the Magnetosphere and Ionosphere

The research facilities on the Svalbard archepelego provide a unique opportunity for observing the temporal and spatial characteristics of the interaction of the solar wind with the magnetosphere-ionosphere system. The first sounding rockets from the SvdRak range at Ny-Alesund have opened a new perspective for separating temporal and spatial effects. Finding a correlation between observations from the rockets and the Wind satellite located nearly 200 R(sub E) upstream in the solar wind that had a lag time less than the advection time forced consideration of tilted phase planes of the interp1anetary electric field. From this it was deduced that the interaction process has to involve high latitude merging, the interplanetary magnetic field (IMF) B(sub Y) bifurcates the cusp relative to the high latitude source regions in the Northern and Southern Hemispheres, IMF B(sub X) controls the interaction time in each hemisphere, and the small convection cell is driven by opposite hemisphere merging. Studies with 4 satellites in the solar wind have shown that the tilt of the phase plane varies on a minute-by-minute basis and change significantly on time scales of tens of minutes. Images of the cusp at 557.7 nm provide a nearly instantaneous picture of the temporal and spatial characteristics of merging at the magnetopause and show that the process can occur at multiple locations. The rate and location vary with time.

Maynard, Nelson C.↗

Development of a Platform for High-Resolution Ion Mobility Separations Coupled with Messenger Tagging Infrared Spectroscopy for High-Precision Structural Characterizations

The ability to uniquely identify a compound requires highly precise and orthogonal measurements. Here we describe a newly developed analytical platform that integrates high resolution ion mobility and cryogenic vibrational ion spectroscopy for high-precision structural characterizations. This platform allows for the temporal separation of isomeric/isobaric ions and provides a highly sensitive description of the ion’s adopted geometry in the gas phase. The combination of these orthogonal structural measurements yields precise descriptors that can be used to resolve between and confidently identify highly similar ions. The unique benefits of our instrument, which integrates a structures for lossless ion manipulations ion mobility (SLIM IM) device with messenger tagging infrared spectroscopy, include the ability to perform high-resolution ion mobility separations and to record the IR spectra of all ions simultaneously. The SLIM IM device, with its 13 m separation path length, allows for multipass experiments to be performed for increased resolution as needed. It is integrated with an Agilent qTOF MS where the collision cell was replaced with a cryogenically held (30 K) TW-SLIM module. The cryo-SLIM is operated in a novel manner that allows ions to be streamed through the device and collisionally cooled to a temperature where they can form noncovalently bound N 2 complexes that are maintained as they exit the device and are detected by the TOF mass analyzer. The instrument can be operated in two modes: IMS+IR where the IR spectra for mobility-selected ions can be recorded and IR-only mode where the IR spectra for all mass-resolved ions can be recorded. In IR-only mode, IR spectra (400 cm –1 spectral range) can be recorded in as short as 2 s for high throughput measurements. Further, this work details the construction of the instrument and modes of operation. It provides initial benchmarking of CCS and IR measurements to demonstrate the utility of this instrument for targeted and untargeted approaches.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Tethered Satellites as Enabling Platforms for an Operational Space Weather Monitoring System

Space weather nowcasting and forecasting models require assimilation of near‐real time (NRT) space environment data to improve the precision and accuracy of operational products. Typically, these models begin with a climatological model to provide "most probable distributions" of environmental parameters as a function of time and space. The process of NRT data assimilation gently pulls the climate model closer toward the observed state (e.g. via Kalman smoothing) for nowcasting, and forecasting is achieved through a set of iterative physics‐based forward‐prediction calculations. The issue of required space weather observatories to meet the spatial and temporal requirements of these models is a complex one, and we do not address that with this poster. Instead, we present some examples of how tethered satellites can be used to address the shortfalls in our ability to measure critical environmental parameters necessary to drive these space weather models. Examples include very long baseline electric field measurements, magnetized ionospheric conductivity measurements, and the ability to separate temporal from spatial irregularities in environmental parameters. Tethered satellite functional requirements will be presented for each space weather parameter considered in this study.

Krause, L. Habash↗

Tethered Satellites as an Enabling Platform for Operational Space Weather Monitoring Systems

Tethered satellites offer the potential to be an important enabling technology to support operational space weather monitoring systems. Space weather "nowcasting" and forecasting models rely on assimilation of near-real-time (NRT) space environment data to provide warnings for storm events and deleterious effects on the global societal infrastructure. Typically, these models are initialized by a climatological model to provide "most probable distributions" of environmental parameters as a function of time and space. The process of NRT data assimilation gently pulls the climate model closer toward the observed state (e.g., via Kalman smoothing) for nowcasting, and forecasting is achieved through a set of iterative semi-empirical physics-based forward-prediction calculations. Many challenges are associated with the development of an operational system, from the top-level architecture (e.g., the required space weather observatories to meet the spatial and temporal requirements of these models) down to the individual instruments capable of making the NRT measurements. This study focuses on the latter challenge: we present some examples of how tethered satellites (from 100s of m to 20 km) are uniquely suited to address certain shortfalls in our ability to measure critical environmental parameters necessary to drive these space weather models. Examples include long baseline electric field measurements, magnetized ionospheric conductivity measurements, and the ability to separate temporal from spatial irregularities in environmental parameters. Tethered satellite functional requirements are presented for two examples of space environment observables.

Gilchrist, Brian E.↗

Composition of the carbohydrate granules of the cyanobacterium, Cyanothece sp. strain ATCC 51142

Cyanothece sp. strain ATCC 51142 is an aerobic, unicellular, diazotrophic cyanobacterium that temporally separates O2-sensitive N2 fixation from oxygenic photosynthesis. The energy and reducing power needed for N2 fixation appears to be generated by an active respiratory apparatus that utilizes the contents of large interthylakoidal carbohydrate granules. We report here on the carbohydrate and protein composition of the granules of Cyanothece sp. strain ATCC 51142. The carbohydrate component is a glucose homopolymer with branches every nine residues and is chemically identical to glycogen. Granule-associated protein fractions showed temporal changes in the number of proteins and their abundance during the metabolic oscillations observed under diazotrophic conditions. There also were temporal changes in the protein pattern of the granule-depleted supernatant fractions from diazotrophic cultures. None of the granule-associated proteins crossreacted with antisera directed against several glycogen-metabolizing enzymes or nitrogenase, although these proteins were tentatively identified in supernatant fractions. It is suggested that the granule-associated proteins are structural proteins required to maintain a complex granule architecture.

NASA Discipline Cell Biology↗

Linkages between terrestrial ecosystems and the atmosphere

The primary research issue in understanding the role of terrestrial ecosystems in global change is analyzing the coupling between processes with vastly differing rates of change, from photosynthesis to community change. Representing this coupling in models is the central challenge to modeling the terrestrial biosphere as part of the earth system. Terrestrial ecosystems participate in climate and in the biogeochemical cycles on several temporal scales. Some of the carbon fixed by photosynthesis is incorporated into plant tissue and is delayed from returning to the atmosphere until it is oxidized by decomposition or fire. This slower (i.e., days to months) carbon loop through the terrestrial component of the carbon cycle, which is matched by cycles of nutrients required by plants and decomposers, affects the increasing trend in atmospheric CO2 concentration and imposes a seasonal cycle on that trend. Moreover, this cycle includes key controls over biogenic trace gas production. The structure of terrestrial ecosystems, which responds on even longer time scales (annual to century), is the integrated response to the biogeochemical and environmental constraints that develop over the intermediate time scale. The loop is closed back to the climate system since it is the structure of ecosystems, including species composition, that sets the terrestrial boundary condition in the climate system through modification of surface roughness, albedo, and, to a great extent, latent heat exchange. These separate temporal scales contain explicit feedback loops which may modify ecosystem dynamics and linkages between ecosystems and the atmosphere. The long-term change in climate, resulting from increased atmospheric concentrations of greenhouse gases (e.g., CO2, CH4, and nitrous oxide (N2O)) will further modify the global environment and potentially induce further ecosystem change. Modeling these interactions requires coupling successional models to biogeochemical models to physiological models that describe the exchange of water, energy, and biogenic trace gases between the vegetation and the atmosphere at fine time scales. There does not appear to be any obvious way to allow direct reciprocal coupling of atmospheric general circulation models (GCM's), which inherently run with fine time steps, to ecosystem or successional models, which have coarse temporal resolution, without the interposition of physiological canopy models. This is equally true for biogeochemical models of the exchange of carbon dioxide and trace gases. This coupling across time scales is nontrivial and sets the focus for the modeling strategy.

Bretherton, Francis↗

Confidence-Based Buffer for Strategic Deconfliction with Probabilistic Operational Intent

This paper presents a methodology to expand the 95% confidence level of the elliptical geometry given by Unmanned Aircraft System (UAS) operators planning to fly Beyond Visual Line of Sight (BVLOS) to any confidence level before being fed to the strategic deconfliction (SD) module, effectively increasing the separation buffer between Operational Intents (OIs). To assess the performance of this approach, it is integrated within an adaptation of the Rolling Horizon with K-Position Search volume-based strategic deconfliction approach, previously developed at NASA Ames, preventing the 4D overlapping of OIs shaped by ellipses instead of traditional blocks. Safety and efficiency metrics are evaluated through the deconfliction of four simulated package delivery route network structures across the San Francisco Metropolitan Area with increasing numbers of crossing waypoints (network complexity). Safety assessment entails the in-house creation of a metric to quantify collision occurrences per flight hour based on the frequency at which the probabilistic operational volume segments are sampled, whereas efficiency is measured using ground delay. Results indicate that the largest buffer growth occurs when increasing the confidence level beyond 99.9% and demonstrate the negative impact of network complexity on both metrics, regardless of the OI geometry. Further, the ellipse-based SD adaptation more accurately estimates temporal separation at crossings, allowing deconflicted vehicles to be closer together. It is concluded that the proposed methodology enables the desired confidence level to serve as an effective controller of buffer size.

strategic deconfliction↗

Confidence-Based Buffer for Strategic Deconfliction with Probabilistic Operational Intent

This paper presents a methodology to expand the 95% confidence level of the elliptical geometry given by Unmanned Aircraft System (UAS) operators planning to fly Beyond Visual Line of Sight (BVLOS) to any confidence level before being fed to the strategic deconfliction (SD) module, effectively increasing the separation buffer between Operational Intents (OIs). To assess the performance of this approach, it is integrated within an adaptation of the Rolling Horizon with K-Position Search volume-based strategic deconfliction approach, previously developed at NASA Ames, preventing the 4D overlapping of OIs shaped by ellipses instead of traditional blocks. Safety and efficiency metrics are evaluated through the deconfliction of four simulated package delivery route network structures across the San Francisco Metropolitan Area with increasing numbers of crossing waypoints (network complexity). Safety assessment entails the in-house creation of a metric to quantify collision occurrences per flight hour based on the frequency at which the probabilistic operational volume segments are sampled, whereas efficiency is measured using ground delay. Results indicate that the largest buffer growth occurs when increasing the confidence level beyond 99.9% and demonstrate the negative impact of network complexity on both metrics, regardless of the OI geometry. Further, the ellipse-based SD adaptation more accurately estimates temporal separation at crossings, allowing deconflicted vehicles to be closer together. It is concluded that the proposed methodology enables the desired confidence level to serve as an effective controller of buffer size.

safety↗

Delayed leaf greening involves a major shift in the expression of cytosolic and mitochondrial ribosomes to plastid ribosomes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)

Abstract Background and aims Hakea prostrata (Proteaceae) is a highly phosphorus-use-efficient plant native to southwest Australia. It maintains a high photosynthetic rate at low leaf phosphorus (P) and exhibits delayed leaf greening, a convergent adaptation that increases nutrient-use efficiency. This study aimed to provide broad physiological and gene expression profiles across leaf development, uncovering pathways leading from young leaves as nutrient sinks to mature leaves as low-nutrient, energy-transducing sources. Methods To explore gene expression underlying delayed greening, we analysed a de novo transcriptome for H. prostrata across five stages of leaf development. Photosynthesis and respiration rates, and foliar pigment, P and nitrogen (N) concentrations were determined, including the division of P into five biochemical fractions. Key results Transcripts encoding functions associated with leaf structure generally decreased in abundance across leaf development, concomitant with decreases in foliar concentrations of 85% for anthocyanins, 90% for P and 70% for N. The expression of genes associated with photosynthetic function increased during or after leaf expansion, in parallel with increases in photosynthetic pigments and activity, much later in leaf development than in species that do not have delayed greening. As leaves developed, transcript abundance for cytosolic and mitochondrial ribosomal proteins generally declined, whilst transcripts for chloroplast ribosomal proteins increased. Conclusions There was a much longer temporal separation of leaf cell growth from chloroplast development in H. prostrata than is found in species that lack delayed greening. Transcriptome-guided analysis of leaf development in H. prostrata provided insight into delayed greening as a nutrient-saving strategy in severely phosphorus-impoverished landscapes.

59 BASIC BIOLOGICAL SCIENCES↗

Stable Tetrasubstituted Quinone Redox Reservoir for Enhancing Decoupled Hydrogen and Oxygen Evolution

In this report, we discuss Redox reservoirs (RRs) that may be used to decouple the two half-reactions of water electrolysis, enabling the spatial and temporal separation of hydrogen and oxygen evolution. Organic RRs are appealing candidates for this application; however, their instability limits their utility. Here we show that a tetrathioether-substituted quinone, tetramercaptopropanesulfonate quinone (TMQ), exhibits significantly enhanced stability relative to anthraquinone-2,7-disulfonate, the most effective organic RR reported previously. The enhanced stability, confirmed by symmetric flow-battery experiments under relevant conditions, enables the stable electrochemical production of H 2 and O 2 in a continuous-flow electrolysis cell. The reduced RR, tetramercaptopropanesulfonate hydroquinone, is not susceptible to decomposition, whereas the oxidized state, TMQ, undergoes slow decomposition, evident only after sustained operation (>60 h). The analysis of the byproducts provides a basis for a decomposition mechanism, establishing a foundation for the design of new organic RRs with even better performance.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Root and Microbial Soil CO 2 and CH 4 Fluxes Respond Differently to Seasonal and Episodic Environmental Changes in a Temperate Forest

Upland forest soils are typically major atmospheric carbon dioxide (CO 2 ) sources and methane (CH 4 ) sinks, but the contributions of root and microbial processes, as well as their separate temporal responses to environmental change, remain poorly understood. This 2-year study was conducted in a temperate, deciduous forest located on the Chesapeake Bay in Maryland, USA. We used temporal CO 2 and CH 4 flux measurements, exclusion-source partitioning, and an ecosystem-scale flooding experiment to understand how carbon (C) fluxes, and their root and microbial sources, respond to seasonal and episodic environmental change. Here, we show that the root-and-rhizosphere component of soil CO 2 and CH 4 flux is significant and that its dependence on soil temperature and volumetric water content (VWC) influences soil C dynamics at seasonal timescales. Experimental flooding shows that CO 2 and CH 4 flux responses to episodic moisture change were driven by suppression of soil heterotrophs, while root respiration did not respond to transient hydrologic disturbance. Methane uptake responded strongly to episodic inundation, reinforcing the important role of soil moisture in the short-term control of the forest soil CH 4 sink. However, despite the clear seasonality of CH 4 uptake, as well as its strong response to short-term experimental inundation, temperature and VWC were weak predictors of CH 4 uptake at a seasonal timescale. We suggest that CH 4 consumption in the long-term may be determined by vegetation, nutrients, microbial communities, or other factors correlated with seasonal changes. Our results indicate that root and microbial sources of both CO 2 and CH 4 flux respond differently in timing and magnitude to seasonal and episodic environmental change.

54 ENVIRONMENTAL SCIENCES↗

Cave Thiovulum ( Candidatus Thiovulum stygium) differs metabolically and genomically from marine species

Thiovulum spp. (Campylobacterota) are large sulfur bacteria that form veil-like structures in aquatic environments. The sulfidic Movile Cave (Romania), sealed from the atmosphere for ~5 million years, has several aqueous chambers, some with low atmospheric O 2 (~7%). The cave’s surface-water microbial community is dominated by bacteria we identified as Thiovulum. We show that this strain, and others from subsurface environments, are phylogenetically distinct from marine Thiovulum. We assembled a closed genome of the Movile strain and confirmed its metabolism using RNAseq. We compared the genome of this strain and one we assembled from public data from the sulfidic Frasassi caves to four marine genomes, including Candidatus Thiovulum karukerense and Ca. T. imperiosus, whose genomes we sequenced. Despite great spatial and temporal separation, the genomes of the Movile and Frasassi Thiovulum were highly similar, differing greatly from the very diverse marine strains. We concluded that cave Thiovulum represent a new species, named here Candidatus Thiovulum stygium. Based on their genomes, cave Thiovulum can switch between aerobic and anaerobic sulfide oxidation using O 2 and NO 3 - as electron acceptors, the latter likely via dissimilatory nitrate reduction to ammonia. Thus, Thiovulum is likely important to both S and N cycles in sulfidic caves. Electron microscopy analysis suggests that at least some of the short peritrichous structures typical of Thiovulum are type IV pili, for which genes were found in all strains. These pili may play a role in veil formation, by connecting adjacent cells, and in the motility of these exceptionally fast swimmers.

59 BASIC BIOLOGICAL SCIENCES↗

Interframe-tunable ultrafast differential-displacement holography

Here we describe the details of a digital holographic microscopy diagnostic capable of quantifying both the topography and velocity of a km/s object with adjustable temporal sensitivity. This technique involves spatially multiplexing a double pulse reflected from a target with reference beams of precisely known temporal separation.

47 OTHER INSTRUMENTATION↗

Integrative analysis of CAM photosynthesis reveals its impact on primary metabolism in Yucca

Crassulacean Acid Metabolism (CAM) is an adaptation that temporally separates carbon uptake at night from photosynthesis during the day. CAM has evolved repeatedly across vascular plants, as its emergence may depend on simple regulatory changes to deeply conserved metabolic pathways. Modern CAM research relies heavily on interpretation of transcriptomic data, though regulation occurs at multiple levels following transcription. Additionally, while most research to date has focused on a handful of genes and metabolites in the core CAM pathway, the co-option of conserved regulatory and functional genes is bound to have wide-ranging effects on other aspects of primary metabolism. In this study, we integrate transcriptomic, proteomic, and metabolomic data to compare primary metabolism between the CAM species Yucca aloifolia and closely related C 3 species, Y. filamentosa. We observe minimal correlation between protein abundance and mRNA expression, suggesting significant post-transcriptional regulation in CAM species. We also find evidence of shifts in gene expression and metabolite accumulation outside of the central CAM pathway, suggesting that the shift to CAM has cascading effects across primary metabolism, especially nitrogen metabolism. Our findings provide insights into the metabolic shifts associated with CAM evolution, and highlight the complexity of its regulation at multiple biological levels.

59 BASIC BIOLOGICAL SCIENCES↗

High-speed fiber-based spectrometer for plasma Thomson scattering

Here we present a novel concept for a Thomson scattering diagnostic, based on a high-speed fiber optic spectrometer. The high-speed fiber optic spectrometer presented here translates a spectral measurement from the frequency domain into the time domain, thus requiring the use of only a single photodetector for spectral acquisition. The high temporal precision offered by the instrument gives rise to a number of advantages over traditional spectrometers, such as nearly background-free measurements and multiple uses of the same injected beam. Multiple uses of the same beam would enable greatly increased measurement rates, in the range of 10–100 MHz. The spectral range and resolution of the fiber spectrometer can be easily tailored to be optimized for the light source and experimental conditions by selecting different lengths of fiber, thus allowing for the proposed technique to exhibit high dynamic range when measuring many points simultaneously. Finally, due to the temporal separation of the background from the signal, these improvements are possible without the need for increased average input laser power.

47 OTHER INSTRUMENTATION↗