Study of time-lapse data processing for dynamic hydrologic conditions
There are no author-identified significant results in this report.
Serebreny, S. M.↗
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Study of time lapse data processing for dynamic hydrologic conditions
There are no author-identified significant results in this report.
Serebreny, S. M.↗
Study of time lapse processing for dynamic hydrologic conditions
There are no author-identified significant results in this report.
Serebreny, S. M.↗
Study of time lapse data processing for dynamic hydrologic conditions
There are no author-identified significant results in this report.
Serebreny, S. M.↗
Time-lapse analysis of ERTS imagery using special electronic viewing/measuring equipment
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Evans, W. E.↗
Exploiting the temporal coherence of repetitive satellite imagery
Imagery from ERTS or the Synchronous Meteorological Satellites, because of its precisely repetitive nature, is ideally suited both for subjective enhancement by time-lapse display and for objective measurements of changes with time. A hybrid system providing both viewing and image-processing functions is described. A representative application involves the measurement of changes in the aerial extent of snow cover within specified mountain watersheds. Subjective viewing of time-lapse sequences of the snow scenes provides valuable insight into the nature of numerous extraneous signals, permitting the interim implementation of operationally useful snow-measuring systems and hopefully leading toward the design of fully objective classifiers.
Evans, W. E.↗
Effects of Angular Frequency During Clinorotation on Mesenchymal Stem Cell Morphology and Migration
Background/Objectives: Ground-based microgravity simulation can reproduce the apparent effects of weightlessness in spaceflight using clinostats that continuously reorient the gravity vector on a specimen, creating a time-averaged nullification of gravity. In this work, we investigated the effects of clinorotation speed on the morphology, cytoarchitecture, and migration behavior of human mesenchymal stem cells (hMSCs). Methods: We compared cell responses at clinorotation speeds of 0, 30, 60, and 75 rpm over 8 hours in a recently developed lab-on-chip-based clinostat system. Time lapse light microscopy was used to visualize changes in cell morphology during and after cessation of clinorotation. Cytoarchitecture was assessed by actin and vinculin staining, and chemotaxis was examined using time lapse light microscopy of cells in NGF (100 ng/ml) gradients. Results: Among clinorotated groups, cell area distributions indicated a greater inhibition of cell spreading with higher angular frequency (p is less than 0.005), though average cell area at 30 rpm after 8 hours became statistically similar to control (p = 0.794). Cells at 75rpm clinorotation remained viable and were able to re-spread after clinorotation. In chemotaxis chambers clinorotation did not alter migration patterns in elongated cells, but most clinorotated cells exhibited cell retraction, which strongly compromised motility.
microgravity↗
Mount St. Helens dust veil observed at Boulder, Colorado by optical techniques
Following the May 18, 1980, eruption of Mount St. Helens, photometric and photograhic observations were taken at Boulder, Colorado, to record the optical effects of volcanic dust atmospherically transported to this area. The instruments used included a narrow-beamwidth solar photometer which recorded solar irradiance in eight narrow-bandwidth channels in the wavelength range 0.3 to 1.1 microns, a solar aureole photometer, and two time-lapse camera systems. The eight-channel solar photometer data have been analyzed to obtain the wavelength dependence of optical thickness. At the longer wavelengths, on May 20, 1980, the optical thickness was as much as nine times that expected from a 'clean atmosphere' model. During the first several days following the eruption, the dust veil sometimes exhibited sufficient spatial structure that its motion can be seen on the time-lapse films. The results of analysis to date are presented and the plans for additional analysis are outlined.
Lerfald, G.↗
McIDAS III - A modern interactive data access and analysis system
A powerful facility for meteorological analysis called the Man Computer Interactive Data Access System (McIDAS) was designed and implemented in the early 1970's at the Space Science and Engineering Center of the University of Wisconsin-Madison. Hardware and software experience gained via extensive use of that facility and its derivatives have led to a newer implementation of McIDAS on a larger computer with significant enhancements to the supporting McIDAS software. McIDAS allows remote and local access to a wide range of data from satellites and conventional observations, time lapse displays of imagery data, overlaid graphics, and current and past meteorological data. Available software allows one to perform analysis of a wide range of digital images as well as temperature and moisture sounding data obtained from satellites. McIDAS can generate multicolor composites of conventional and satellite weather data, radar and forecast data in a wide variety of two- and three-dimensional displays as well as time lapse movies of these analyses. These and other capabilities are described in this paper.
Suomi, V. E.↗
Physiological Maturation of Regenerating Hair Cells
The bullfrog saccule, a sensor of gravity and substrate-borne vibration, is a model system for hair cell transduction. Saccular hair cells also increase in number throughout adult life and rapidly recover after hair cell damage, making this organ an ideal system for studying hair cell development, repair, and regeneration. We have used of hair cell and supporting cell immunocytochemical markers to identify damaged hair cells and hair cell precursors in organotypic cultures of the bullfrog saccule. We then used an innovative combination of confocal, electron, and time-lapse microscopy to study the fate of damaged hair cells and the origin of new hair cells after gentamicin ototoxicity in normal and mitotically blocked saccular cultures. These studies have shown that gentamicin ototoxicity produces both lethal and sublethal hair cell damage. They have also shown that hair cell recovery in this organ takes place by both the repair of sublethally damaged hair cells and by the replacement of lost hair cells by mitotic regeneration. In parallel studies, we have used biophysical and molecular biological techniques to study the differentiation and innervation of developing, repairing, and regenerating hair cells. More specifically, we have used RT-PCR to obtain the bullfrog homologues of L-type voltage- gated calcium (L-VGCC) and large-conductance Ca(2+)-activated potassium (BK) channel genes. We have then obtained probes for these genes and, using in situ hybridization, begun to examine their expression in the bullfrog saccule and amphibian papilla. We have also used fluorescent-labeled channel toxins and channel toxin derivatives to determine the time of appearance of L-type voltage-gated calcium (L-VGCC) and Ca(2+)-activated potassium (BK) channels and to study dynamic changes in the number, distribution, and co-localization of these proteins in developing, repairing, and regenerating hair cells. Using time-lapse microscopy, we are also studying the dynamic relationship between ion channel clustering and synaptic formation in hair cells and afferent neurons. In future studies, we will determine when hair cell precursors acquire electrical tuning, and, using whole-cell patch-clamp techniques, identify and characterize their L-VGCC and BK currents. We will also use biophysical techniques to determine the number of L-VGCC and BK channels and the size and gating kinetics of their underlying L-VGCC and BK conductances, correlating these variables with the amplitude and frequency of membrane oscillations produced by intracellular current steps. We expect these studies to determine how hair cells regulate ion channel expression to achieve specific physiological responses.
Baird, Richard A.↗
Linear response theory for annealing of radiation damage in semiconductor devices
A theoretical study of the radiation/annealing response of MOS ICs is described. Although many experiments have been performed in this field, no comprehensive theory dealing with radiation/annealing response has been proposed. Many attempts have been made to apply linear response theory, but no theoretical foundation has been presented. The linear response theory outlined here is capable of describing a broad area of radiation/annealing response phenomena in MOS ICs, in particular, both simultaneous irradiation and annealing, as well as short- and long-term annealing, including the case when annealing is nearing completion. For the first time, a simple procedure is devised to determine the response function from experimental radiation/annealing data. In addition, this procedure enables us to study the effect of variable temperature and dose rate, effects which are of interest in spaceflight. In the past, the shift in threshold potential due to radiation/annealing has usually been assumed to depend on one variable: the time lapse between an impulse dose and the time of observation. While such a suggestion of uniformity in time is certainly true for a broad range of radiation annealing phenomena, it may not hold for some ranges of the variables of interest (temperature, dose rate, etc.). A response function is projected which is dependent on two variables: the time of observation and the time of the impulse dose. This dependence on two variables allows us to extend the theory to the treatment of a variable dose rate. Finally, the linear theory is generalized to the case in which the response is nonlinear with impulse dose, but is proportional to some impulse function of dose. A method to determine both the impulse and response functions is presented.
Litovchenko, Vitaly↗
Bacteria Counter
Science Applications, Inc.'s ATP Photometer makes a rapid and accurate count of the bacteria in a body fluid sample. Instrument provides information on the presence and quantity of bacteria by measuring the amount of light emitted by the reaction between two substances. Substances are ATP adenosine triphosphate and luciferase. The reactants are applied to a human body sample and the ATP Photometer observes the intensity of the light emitted displaying its findings in a numerical output. Total time lapse is usually less than 10 minutes, which represents a significant time savings in comparison of other techniques. Other applications are measuring organisms in fresh and ocean waters, determining bacterial contamination of foodstuffs, biological process control in the beverage industry, and in assay of activated sewage sludge.
Source record↗
Building a Bilingual Google Earth Engine Dashboard to Increase Accessibility to Long-term Time Series Remote Sensing Data for Monitoring Saline System Changes in Chile’s Atacama Desert
Saline systems, consisting of salt flats, ponds, and marshes, provide vital water resources to wildlife and communities in northern Chile’s Atacama Desert, one of the driest regions in the world. Mining is extensive in the Atacama, which contains 30% of the world’s lithium reserves and is abundant in potassium and boron. The groundwater that feeds into salt marshes and ponds is extracted in large volumes for mining operations, limiting the availability of water for ecosystems. However, identifying long-term and large-scale environmental impacts from local lithium mining on the saline systems is limited by region inaccessibility and terrain variability. Open access satellite imagery and cloud computing technology has made studying Atacama saline systems feasible and allowed for collaboration across different agencies and countries. The NASA DEVELOP Program partnered with Chile’s la Universidad de La Serena and Servicio Nacional de Geología y Minería (SERNAGEOMIN) to create the Saline Analysis Tool (SalT) in Google Earth Engine (GEE). SalT is used to analyze the extent and distribution of remote saline systems in the Atacama from 1986 to the present day. The tool filters Landsat 5 Thematic Mapper (TM) and Landsat 8 Operational Land Imager (OLI) data from GEE’s data catalog and creates a single composite image per year for analysis. Additional output analyses include land cover classification, Normalized Difference Vegetation Index (NDVI) and Normalized Difference Water Index (NDWI) raster images that can be displayed on the map interface or exported. The tool can also generate time-lapse videos and charts displaying NDVI, NDWI, and land cover over time. A key feature of the tool is the use of a bilingual graphical user interface to make analysis accessible and customizable to different users’ needs—SalT provides options to select an analysis area, analysis time period, and outputs to display or export. The tool also incorporates new Earth observations as they are added to GEE’s catalog. The ability to easily visualize and analyze long-term remote sensing imagery will enable SERNAGEOMIN and la Universidad de la Serena to continually monitor changes in these saline systems and inform future land management policy.
NASA DEVELOP↗
Lab-On-Chip Clinorotation System for Live-Cell Microscopy Under Simulated Microgravity
Cells in microgravity are subject to mechanical unloading and changes to the surrounding chemical environment. How these factors jointly influence cellular function is not well understood. We can investigate their role using ground-based analogues to spaceflight, where mechanical unloading is simulated through the time-averaged nullification of gravity. The prevailing method for cellular microgravity simulation is to use fluid-filled containers called clinostats. However, conventional clinostats are not designed for temporally tracking cell response, nor are they able to establish dynamic fluid environments. To address these needs, we developed a Clinorotation Time-lapse Microscopy (CTM) system that accommodates lab-on- chip cell culture devices for visualizing time-dependent alterations to cellular behavior. For the purpose of demonstrating CTM, we present preliminary results showing time-dependent differences in cell area between human mesenchymal stem cells (hMSCs) under modeled microgravity and normal gravity.
Yew, Alvin G.↗
Lab-On-Chip Clinorotation System for Live-Cell Microscopy Under Simulated Microgravity
Cells in microgravity are subject to mechanical unloading and changes to the surrounding chemical environment. How these factors jointly influence cellular function is not well understood. We can investigate their role using ground-based analogues to spaceflight, where mechanical unloading is simulated through the time-averaged nullification of gravity. The prevailing method for cellular microgravity simulation is to use fluid-filled containers called clinostats. However, conventional clinostats are not designed for temporally tracking cell response, nor are they able to establish dynamic fluid environments. To address these needs, we developed a Clinorotation Time-lapse Microscopy (CTM) system that accommodates lab-on- chip cell culture devices for visualizing time-dependent alterations to cellular behavior. For the purpose of demonstrating CTM, we present preliminary results showing time-dependent differences in cell area between human mesenchymal stem cells (hMSCs) under modeled microgravity and normal gravity.
Yew, Alvin G.↗
Studies of Model Immiscible Systems
The objectives are to use model transparent monotectics to obtain fundamental information applicable to two-phase systems in general, to apply this understanding to materials of interest in the Microgravity Science and Applications program, and to interpret results of flight experimental involving monotectic alloys. A number of model immiscible systems are in use to study various aspects of two-phase behavior within the miscibility gap and during solidification. Particle growth, coalescence and particle motions are under investigation using a holographic microscopy system. The system is capable of working with particle densities up to 10 to the 7th power particles/cubic centimeters through a 100 micron depth and can resolve particles of the order of 2 to 3 micron in diameter throughout the entire cell volume. Particle size, distribution changes with respect to time and temperature are observable from sequential holograms. Initial experiments using diethylene glycol/ethyl salicylate (DEG/ES) have demonstrated the usefulness of the technique. The thermal system controls temperature to at least plus or minus 0.001 K over the course of an experiment. A time-lapse film, made from holograms, of a succinonitrile/water solution shows particle size and number distribution changes with time under isothermal conditions. The observations are consistent with Ostwald ripening theory.
Frazier, D. O.↗
Plant Growth Biophysics: the Basis for Growth Asymmetry Induced by Gravity
The identification and quantification of the physical properties altered by gravity when plant stems grow upward was studied. Growth of the stem in vertical and horizontal positions was recorded by time lapse photography. A computer program that uses a cubic spline fitting algorithm was used to calculate the growth rate and curvature of the stem as a function of time. Plant stems were tested to ascertain whether cell osmotic pressure was altered by gravity. A technique for measuring the yielding properties of the cell wall was developed.
Cosgrove, D.↗
The pointing errors of geosynchronous satellites
A study of the correlation between cloud motion and wind field was initiated. Cloud heights and displacements were being obtained from a ceilometer and movie pictures, while winds were measured from pilot balloon observations on a near-simultaneous basis. Cloud motion vectors were obtained from time-lapse cloud pictures, using the WINDCO program, for 27, 28 July, 1969, in the Atlantic. The relationship between observed features of cloud clusters and the ambient wind field derived from cloud trajectories on a wide range of space and time scales is discussed.
Sikdar, D. N.↗