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DOE OSTI2021

A bioinspired and chemically defined alternative to dimethyl sulfoxide for the cryopreservation of human hematopoietic stem cells

Abstract The cryopreservation of hematopoietic cells using dimethyl sulfoxide (DMSO) and serum is a common procedure used in transplantation. However, DMSO has clinical and biological side effects due to its toxicity, and serum introduces variation and safety risks. Inspired by natural antifreeze proteins, a novel class of ice-interactive cryoprotectants was developed. The corresponding DMSO-, protein-, and serum-free cryopreservation media candidates were screened through a series of biological assays using human cell lines, peripheral blood cells, and bone marrow cells. XT-Thrive-A and XT-Thrive-B were identified as lead candidates to rival cryopreservation with 10% DMSO in serum based on post-thaw cell survival and short-term proliferation assays. The effectiveness of the novel cryopreservation media in freezing hematopoietic stem cells from human whole bone marrow was assessed by extreme limiting dilution analysis in immunodeficient mice. Stem cell frequencies were measured 12 weeks after transplant based on bone marrow engraftment of erythroid, myeloid, B-lymphoid, and CD34 + progenitors measured by flow cytometry. The recovered numbers of cryopreserved stem cells were similar among XT-Thrive A, XT-Thrive B, and DMSO with serum groups. These findings show that cryoprotectants developed through biomimicry of natural antifreeze proteins offers a substitute for DMSO-based media for the cryopreservation of hematopoietic stem cells.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2023

Energy transport analysis of NSTX plasmas with the TGLF turbulent and NEO neoclassical transport models

This work presents a study of plasma transport at low aspect ratio on the National Spherical Torus Experiment tokamak, where the turbulent and neoclassical energy fluxes calculated by the quasilinear Trapped Gyro Landau Fluid (TGLF) model and the multi species drift-kinetic Neoclassical solver (NEO) are validated against experimental data. The turbulent energy transport of two plasma discharges, one in the L-mode confinement regime and another in the H-mode regime, is dominated by electrostatic drift-wave instabilities, while the ion heat transport has a significant neoclassical contribution. The data analysis workflow is described in detail to understand how the variations of mapping and fitting of experimental data affect the power balance solution and subsequent flux-matching plasma profile predictions with the TGYRO solver. On average, the predicted plasma profiles are consistent with experimental data. However, the solutions are sensitive to various input parameters, including boundary conditions, and the electron-ion coupling. Linear gyrokinetic stability analysis demonstrates close agreement of the real frequencies of unstable modes between TGLF and CGYRO gyrokinetic simulations, but higher growth rates are predicted by TGLF, especially for the H-mode case. Estimates of the low-k modes' contributions to the total flux are consistent with linear stability analysis and the E × B suppression of turbulence in TGLF simulations with the SAT1 saturation model, while the SAT2 saturation model over-predicts the low-k modes' contribution in the H-mode case. Moreover, the results with SAT1 model are consistent with power balance analysis, which indicates only neoclassical ion energy fluxes inside ρ < 0.4 in the L-mode case and $\rho \unicode{x2A7D} 0.7$ in the H-mode case. The presence of multi-scale turbulence and ion-scale driven zonal flow mixing effects are also observed in TGLF scans of the electron turbulent heat flux over a range of temperature gradients and the electron-ion temperature ratio, which could explain the strong model sensitivity to variations of input parameters.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

DOE OSTI2020

Polarity dependent heating at the phase interface in metal-insulator transitions

We show that in a model of a metal photoexcited by a transient pump pulse resonant with a phonon mode, the absolute dc conductivity may become negative, depending on the interplay between the electronic structure, the phonon frequency, and the pump intensity. The analysis includes the effects of inelastic scattering and thermal relaxation. Results for the time evolution of the negative conductivity state are presented; the associated nonequilibrium physics may persist for long times after the pulse. Furthermore, our findings provide a theoretical justification for previously proposed phenomenology and indicate new routes to the generation and exploration of intrinsically nonequilibrium states.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

DOE OSTI2020

Recent Development of Frequency Estimation Methods for Future Smart Grid

The frequency estimated by the Phasor Measurement Unit (PMU) is a critical index of power system status and supports many smart grid applications. The future smart grid features high penetration of renewables and more fast-moving power electronics inverters but raises challenges to the reliable frequency estimation. This article presents three methods to address these challenges. First, an enhanced zero-crossing algorithm was developed to track the fast-changing frequency in system dynamics. Second, we propose a technology that can tolerate the system transient and suppress the outliers. Third, an algorithm was developed to export high time-resolution frequency estimations with minimum computational effort. All of the proposed methods are realized in hardware and compared with classical frequency estimation methods. The testing results indicate that the proposed methods have excellent performance. They can be used in future PMUs and provide reliable and high time resolution data for smart grid applications.

24 POWER TRANSMISSION AND DISTRIBUTION↗

DOE OSTI2024

Correlation Between Microscopic Current Fluctuations Observed at Ultra-Microelectrodes and Macroscopic Bulk Electrolysis Performance in Redox-Active Microemulsions

Microemulsions (μEs) have been proposed as redox flow battery (RFB) electrolytes that maximize ionic conductivity and charge capacity by synergizing two immiscible phases. However, charge transfer during electrolysis in μEs is poorly understood. Here, we show that ultramicroelectrode electrolysis of ferrocene-loaded μEs –20%, 60%, and 90% water - reveals stochastic current fluctuations. These are differentiated in the scanning electrochemical microscopy (SECM) geometry, where power spectral density analysis showed distinct changes in the frequency contributions. SECM in the substrate generation-tip collection mode showed that fluctuations arise under mass-transfer control. Significant differences in the diffusion coefficient of ferrocene species were deducted from SECM approach curves, suggesting phase transfer behavior. Using bulk electrolysis, we calculated the charge accessibility and cycling behavior in the μEs. A decrease in the stochastic behavior of the μEs seems to correlate to a higher accessibility and cycling performance, with the 90% water μE displaying the best reversibility and the 60% the lowest. Altogether, these results suggest that Marangoni-type convection driven by concentration gradients and/or μE restructuring during charge transfer play a role in the electrochemical performance of μEs. This presents opportunities for screening and diagnosing the performance of these emerging RFB electrolytes.

25 ENERGY STORAGE↗

DOE OSTI2023

A time-dependent momentum-resolved scattering approach to core-level spectroscopies

While new light sources allow for unprecedented resolution in experiments with X-rays, a theoretical understanding of the scattering cross-section is lacking. In the particular case of strongly correlated electron systems, numerical techniques are quite limited, since conventional approaches rely on calculating a response function (Kramers-Heisenberg formula) that is obtained from a perturbative analysis of scattering processes in the frequency domain. This requires a knowledge of a full set of eigenstates in order to account for all intermediate processes away from equilibrium, limiting the applicability to small tractable systems. In this work, we present an alternative paradigm, recasting the problem in the time domain and explicitly solving the time-dependent Schrödinger equation without the limitations of perturbation theory: a faithful simulation of the scattering processes taking place in actual experiments, including photons and core electrons. We show how this approach can yield the full time and momentum resolved Resonant Inelastic X-Ray Scattering (RIXS) spectrum of strongly interacting many-body systems. We demonstrate the formalism with an application to Mott insulating Hubbard chains using the time-dependent density matrix renormalization group method, which does not require a priory knowledge of the eigenstates and can solve very large systems with dozens of orbitals. This approach can readily be applied to systems out of equilibrium without modification and generalized to other spectroscopies.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

DOE OSTI2021

Graupel and Hail Identification Algorithm for the Dual-frequency Precipitation Radar (DPR) on the GPM Core Satellite

Precipitation consists of many types of hydrometeors, such as raindrops, ice crystals, graupel, and hail. Due to their impacts, graupel and hail (GH) have received particular attention in the literature. Global Precipitation Measurement (GPM) dual-frequency radar (DPR) has proved to be a very reliable system for global precipitation retrievals. This paper aims to develop a GH identification algorithm for GPM DPR. This algorithm is constructed using a precipitation type index (PTI) defined for DPR. The PTI is effective in separating hydrometeor types and is calculated using measurements of reflectivity, dual-frequency ratio, and storm top height data. The output of the algorithm is a Boolean product representing the existence of graupel or hail along with the vertical profile for each Ku- and Ka-band matched footprint. Cross validation is performed with the Weather Service Radar (WSR-88D) network over continental United States as well as during the Remote sensing of Electrification, Lightning, and Mesoscale/Microscale Processes with Adaptive Ground Observations (RELAMPAGO) experiment. Evaluation of the GH identification algorithm is performed on a global basis, which illustrates promising comparisons with the global lightning and hail precipitation maps generated using radar and radiometer.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2022

Cyber-Physical Dynamic System (CPDS) Modeling for Frequency Regulation and AGC Services of Distributed Energy Resources

The substantial integration of renewable energy brings significant challenges to balance the system in real time because of the variability and intermittency of renewable power. For the reliable system operation, the frequency regulation service is used to stabilize the system frequency through automatically balancing the system generation and load. On one hand, with the substantially increasing deployment of renewable energy in electricity system, the requirement of frequency regulation (FR) services increases significantly. On the other hand, the current main resource of FR services, the controllable conventional generation, is continuously decreasing in the system generation mix. This means that in the future high renewable penetration power system, additional and alternative reliable FR services providers such as distributed energy storage (DES) resources should be explored. Although the capability of utility-scale energy storage to provide FR services has been demonstrated, the integrated control and dynamic modeling of distributed energy resource (DER) providing frequency regulation grid services has been rarely explored. There are several challenges to adopt DERs to provide reliable grid services as illustrated in FERC 755. First, the distributed installation of DES requires a comprehensive cyber-physical dynamic system (CPDS) modeling to fully consider the impacts of the communication latency variability on its real time FR provision capability. Unlike the conventional generators, there are two-layer communication between DERs and system operators which increase the communication delay. Second, unlike the conventional generators whose dynamic models have been comprehensively studied, the difference among individual DER components' power-dynamic characteristic brings challenges in the accurate dynamic AGC modeling of its power-frequency relationship. Third, the temporal dependent state of charge uncertainty of DERs challenges DERs' power and frequency regulation capacities scheduling in the look-ahead generation scheduling. Therefore, the aggregator should optimize the frequency regulation provision from individual DER in real time. To overcome these challenges, this project proposes a cyber-physical dynamic system (CPDS) model to handle the uncertainty of DERs two-layer communication latency and power dynamics. The variability of DERs' communication delay and dynamic constraints will be comprehensively modeled. The DERs' AGC model with communication delay is designed to validate DESs' frequency regulation services. Like current performance-based frequency regulation evaluation, the delivery of DERs' frequency regulation will be assessed through post-analysis of the actual AGC response with respect to the AGC control signal from system operators. Consequently, the reliability improvement with DERs providing reliable frequency regulation services can be evaluated from a comprehensive perspective considering all the dynamics of communication and power.

24 POWER TRANSMISSION AND DISTRIBUTION↗

DOE OSTI2025

Block Island Noise Modeling Data

Noise propagation near Block Island was simulated to assess environmental impacts of impact pile driving during wind turbine construction. Computational models complement field-recorded acoustic data, providing insights into sound attenuation, spectral variability, and propagation dynamics. The dataset includes: 1. Propagation Models: Simulated underwater sound fields documenting sound pressure and directional variability across frequency bands and distances. 2. Spectral Analysis (LTSA): Long-term averages and processed outputs calculating acoustic intensity over time. 3. Visualization Files: Graphs, 2D/3D simulation results, and reference calculations used in sound modeling.

17 WIND ENERGY↗

DOE OSTI2025

A Measurement of the Largest-scale CMB E -mode Polarization with CLASS

We present measurements of large-scale cosmic microwave background E-mode polarization from the Cosmology Large Angular Scale Surveyor 90 GHz data. Using 115 det-yr of observations collected through 2024 with a variable-delay polarization modulator, we achieved a polarization sensitivity of 82 μK arcimin, comparable to Planck at similar frequencies (100 and 143 GHz ). The analysis demonstrates effective mitigation of systematic errors and addresses challenges to large-angular-scale power recovery posed by time-domain filtering in maximum-likelihood map-making. A novel implementation of the pixel-space transfer matrix is introduced, which enables efficient filtering simulations and bias correction in the power spectrum using the quadratic cross-spectrum estimator. Overall, we achieved an unbiased time-domain filtering correction to recover the largest angular scale polarization, with the only power deficit, arising from map-making nonlinearity, being characterized as <3%. Through cross-correlation with Planck, we detected the cosmic reionization at 99.4% significance and measured the reionization optical depth τ = $0.053^{+0.018}_{-0.019}$, marking the first ground-based attempt at such a measurement. At intermediate angular scales (ℓ > 30), our results, both independently and in cross-correlation with Planck, remain fully consistent with Planck’s measurements.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2024

Droplet collection efficiencies inferred from satellite retrievals constrain effective radiative forcing of aerosol–cloud interactions

Abstract. Process-oriented observational constraints for the anthropogenic effective radiative forcing due to aerosol–cloud interactions (ERFaci) are highly desirable because the uncertainty associated with ERFaci poses a significant challenge to climate prediction. The contoured frequency by optical depth diagram (CFODD) analysis supports the evaluation of model representation of cloud liquid-to-rain conversion processes because the slope of a CFODD, generated from joint MODerate Resolution Imaging Spectroradiometer (MODIS)-CloudSat cloud retrievals, provides an estimate of cloud droplet collection efficiency in single-layer warm liquid clouds. Here, we present an updated CFODD analysis as an observational constraint on the ERFaci due to warm rain processes and apply it to the U.S. Department of Energy's Energy Exascale Earth System Model version 2 (E3SMv2). A series of sensitivity experiments shows that E3SMv2 droplet collection efficiencies and ERFaci are highly sensitive to autoconversion, i.e., the rate of mass transfer from cloud liquid to rain, yielding a strong correlation between the CFODD slope and the shortwave component of ERFaci (ERFaciSW; Pearson's R=-0.91). E3SMv2's CFODD slope (0.20 ± 0.04) is in agreement with observations (0.20 ± 0.03). The strong sensitivity of ERFaciSW to the CFODD slope provides a useful constraint on highly uncertain warm rain processes, whereby ERFaciSW, constrained by MODIS-CloudSat, is estimated by calculating the intercept of the linear association between the ERFaciSW and the CFODD slopes, using the MODIS-CloudSat CFODD slope as a reference.

Beall, Charlotte M. (ORCID:0000000311370835)↗

DOE OSTI2022

pyvisco [SWR-22-30]

pyvisco is a Python library that supports the identification of Prony series parameters for linear viscoelastic materials described by a Generalized Maxwell model. The necessary material model parameters are identified by fitting a Prony series to the experimental measurement data. pyvisco allows for the identification of Prony series parameters from experimental data measured in either the frequency-domain (via Dynamic Mechanical Thermal Analysis) or time-domain (via relaxation measurements). The experimental data can be provided as raw measurement sets at different temperatures or as pre-processed master curves. An optional minimization routine is included to reduce the number of Prony elements. This routine is helpful in Finite Element simulations where reducing the computational complexity of the linear viscoelastic material models can shorten the simulation time. See also, https://pypi.org/project/pyvisco/

Springer, Martin↗

DOE OSTI2020

Geothermal Sector Cybersecurity Vulnerability Assessment

A review of geothermal sector-specific cybersecurity vulnerabilities and risks (consequences) was conducted at the request of the Geothermal Technologies Office (GTO). The vulnerabilities and risks reviewed in this study have relevance to achieving the 2019 GeoVision Report (DOE GTO 2019) technological advancements and expected sector growth. The study offers areas for consideration but does not quantify the likelihood (frequency) of the consequences. This cybersecurity analysis project represents a proactive effort to identify areas to enhance cybersecurity in geothermal development and operations. It was not initiated to address any immediate threat or specific known risk. Of the eight identified vulnerabilities analyzed, the review identified reservoir data system monitoring as one that is unique to geothermal systems and may warrant further investigation to better understand risk and mitigation. A detailed analysis of the other vulnerabilities may highlight additional uniqueness relative to other industries. Further research actions are recommended to better quantify risk and enhance cybersecurity preparedness of the sector. As the geothermal industry grows, the cybersecurity strategies to be deployed will be of increasing importance to ensure resilient, reliable, and secure clean energy for years to come.

cyber-physical security↗

DOE OSTI2022

Method and apparatus for measuring optical pulses

A pulse analysis system or method includes a frequency filter that receives an ultrafast pulse under test and disperses the pulse under test over a frequency range. The frequency filter separates the pulse under test into component frequency slices and provides the frequency slices to a detector coupled to a digitizer, which processes the digitized signal and collects a sonogram characteristic of the pulse under test. The frequency slices are arranged to overlap. Ptychography is performed on the sonogram to obtain characteristics of the pulse under test.

Kane, Daniel J.↗

DOE OSTI2023

Method and apparatus for measuring optical pulses

Kane, Daniel J.↗

DOE OSTI2022

Transport and losses of fusion-born alpha particles in the presence of tearing modes using the new Toroidal Accelerated Particle Simulator (TAPaS)

Here, the transport and losses of fusion-born alpha particles are studied in the presence of a single-helicity tearing mode, characterized by (m = 2, n = 1). The analysis is performed by means of the recently developed Toroidal Accelerated Particle Simulator (TAPaS). Although such modes have usually been believed to result only in a local flattening of the radial profiles, it is shown that the density profile can exhibit a global modification leading to significant losses of alpha particles. This is due to the fact that, although the magnetic field does not exhibit any chaotic behaviour, the trajectories of alpha particles do, as revealed by their Poincaré maps. Such results are in qualitative agreement with past observations and simulations of energetic particles generated by neutral beam injection in TFTR, DIII-D and AUG tokamaks. In-depth analysis is carried out to characterize the impact of the tearing mode on the transport and losses of fusion-born alpha-particles with a realistic density profile. The impact of the amplitude is evidenced. Moreover, the effect of the island rotation frequency is assessed based on a detailed analysis of the linear resonances in phase-space, in agreement with the simulation results. Finally, the probability density function of the exit time has been computed and the transport of alpha particles has been found to be anomalous.

anomalous transport↗

DOE OSTI2021

Analysis of Loss-of-Offsite-Power Events: Update

Loss-of-offsite power (LOOP) can have a negative impact on a nuclear power plant’s ability to achieve and maintain safe shutdown conditions. LOOP event frequencies and times required for subsequent restoration of offsite power are important inputs to plant probabilistic risk assessments. This report presents a statistical and engineering analysis of LOOP frequencies and durations at U.S. commercial nuclear power plants. The data used in this study were based on the operating experience during calendar years 1987–2020, while the most recent 15-year data (i.e., from 2006–2020) were used for most analyses in this report. LOOP events during critical operation that did not result in a reactor trip are not included. Frequencies and durations were determined for four LOOP event categories: plant-centered, switchyard-centered, grid-related, and weather related. Highly significant decreasing trends in the LOOP occurrence rates were identified for All-LOOPs during critical operation (p-value = 0.001) and switchyard-centered LOOPs during critical operation (p-value = 0.002) for the most recent 10-year period (2011–2020). Adverse trends in LOOP durations continue for switchyard-centered LOOPs (p-value = 0.005), All-LOOPs (p-value = 0.019), as well as All-LOOPs during shutdown operation (p-value = 0.003). Statistical tests show the LOOP counts are not uniformly distributed across the 12 months, and variation among the months exists for plant-centered LOOPs (p-value = 0.028), grid-related LOOPs (p-value = 0.021), All-LOOPs (p-value = 0.003), and All-LOOPs during critical operation (p-value = 0.008). The engineering analysis of LOOP data showed for the period of 2006–2020, the equipment failure events were dominated by failures of circuits and relay; human errors have been less frequent and occurred primarily in maintenance; and weather events were dominated by tornadoes and lightning.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

DOE OSTI2022

Analysis of Loss-of-Offsite-Power Events: 2021 Update

Loss-of-offsite power (LOOP) can have a negative impact on a nuclear power plant’s ability to achieve and maintain safe shutdown conditions. LOOP event frequencies and times required for subsequent restoration of offsite power are important inputs to plant probabilistic risk assessments. This report presents a statistical and engineering analysis of LOOP frequencies and durations at U.S. commercial nuclear power plants. The data used in this study were based on the operating experience during calendar years 1987–2021, while the most recent 15-year data (i.e., from 2007–2021) were used for most analyses in this report. LOOP events during critical operation that did not result in a reactor trip are not included. Frequencies and durations were determined for four LOOP event categories: plant-centered, switchyard-centered, grid-related, and weather-related. These categories (and the All-LOOPs group which contains all LOOPs without regarding of the four categories) could be further grouped by whether a LOOP event occurred during critical operation, during shutdown operation, or during all operations. The following decreasing trends in the LOOP occurrence rates were identified for the most recent 10-year period (2012–2021): All-LOOPs during critical operation, switchyard-centered LOOPs during critical operation, and grid-related LOOPs during critical operation. Adverse trends in LOOP durations continue for switchyard-centered LOOPs during all operations, All-LOOPs during all operations, and All-LOOPs during shutdown operation for the 1997–2021 period. Statistical tests show the LOOP counts for the period of 2007–2021 are not uniformly distributed across the 12 months, and variation among the months exists for grid-related LOOPs during all operations, All-LOOPs during all operations, and All-LOOPs during critical operation. The engineering analysis of LOOP data showed for the period of 2007–2021, the equipment failure events were dominated by failures of relay and other; human errors have been less frequent and occurred primarily in maintenance and switching; and weather were dominated by tornadoes then by lightning and hurricane. Weather was the cause for 45% of LOOPs for the last fifteen years (2007– 2021) but only for 20% of LOOPs for the previous 20 years (1987–2006) .

22 GENERAL STUDIES OF NUCLEAR REACTORS↗