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At least 379 records · Page 21

Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells

The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985. c 2001. Elsevier Science Ltd. All rights reserved.

NASA Discipline Cell Biology↗

Structural Concepts Study of Non-circular Fuselage Configurations

A preliminary study of structural concepts for noncircular fuselage configurations is presented. For an unconventional flying-wing type aircraft, in which the fuselage is inside the wing, multiple fuselage bays with non-circular sections need to be considered. In a conventional circular fuselage section, internal pressure is carried efficiently by a thin skin via hoop tension. If the section is non-circular, internal pressure loads also induce large bending stresses. The structure must also withstand additional bending and compression loads from aerodynamic and gravitational forces. Flat and vaulted shell structural configurations for such an unconventional, non-circular pressurized fuselage of a large flying-wing were studied. A deep honeycomb sandwich-shell and a ribbed double-wall shell construction were considered. Combinations of these structural concepts were analyzed using both analytical and simple finite element models of isolated sections for a comparative conceptual study. Weight, stress, and deflection results were compared to identify a suitable configuration for detailed analyses. The flat sandwich-shell concept was found preferable to the vaulted shell concept due to its superior buckling stiffness. Vaulted double-skin ribbed shell configurations were found to be superior due to their weight savings, load diffusion, and fail-safe features. The vaulted double-skin ribbed shell structure concept was also analyzed for an integrated wing-fuselage finite element model. Additional problem areas such as wing-fuselage junction and pressure-bearing spar were identified.

Mukhopadhyay, Vivel↗

MSFC Stream Model Preliminary Results: Modeling the 1998-2002 Leonid Encounters and the 1993,1994, and 2004 Perseid Encounters

The cometary meteoroid ejection models of Jones (1996) and Crifo (1997) were used to simulate ejection from comets 55P/Tempel-Tuttle during the last 12 revolutions, and the 1862, 1737, and 161 0 apparitions of 1 OSP/Swift-Tuttle. Using cometary ephemerides generated by the JPL HORIZONS Solar System Data and Ephemeris Computation Service, ejection was simulated in 1 hour time steps while the comet was within 2.5 AU of the Sun. Also simulated was ejection occurring at the hour of perihelion passage. An RK4 variable step integrator was then used to integrate meteoroid position and velocity forward in time, accounting for the effects of radiation pressure, Poynting-Robertson drag, and the gravitational forces of the planets, which were computed using JPL's DE406 planetary ephemerides. An impact parameter is computed for each particle approaching the Earth, and the results are compared to observations of the 1998-2002 Leonid showers, and the 1993-1 994 Perseids. A prediction for Earth's encounter with the Perseid stream in 2004 is also presented.

Moser, D. E.↗

Prevention of hypercalciuria and stone-forming propensity during prolonged bedrest by alendronate

The bone loss and hypercalciuria induced by immobilization or the decreased gravitational forces of space are well described. Using a model of bedrest immobilization, the ability of a potent aminobisphosphonate, alendronate, to avert hypercalciuria and stone-forming propensity was tested. Sixteen male subjects participated in a randomized, placebo-controlled trial in which they received either 20 mg of alendronate or placebo 2 weeks prior to and during 3 weeks of strict bedrest. Parameters of bone and calcium metabolism and urinary crystallization of stone-forming salts were measured before and at the end of bedrest. In the placebo group, bedrest increased urinary calcium (209 +/- 47 to 267 +/- 60 mg/day, p < 0.01) and the saturation of calcium phosphate. Before bedrest, the alendronate group had a significantly lower serum calcium (8.8 +/- 0.4 vs. 9.6 +/- 0.5 mg/dl, p < 0.01) and higher serum PTH (62.4 +/- 33.1 vs. 23.1 +/- 7.5 pg/ml, p < 0.01) compared with the placebo group. Moreover, the alendronate group had a lower urinary calcium (75 +/- 41 mg/day) and saturation of calcium oxalate and calcium phosphate. These effects of alendronate were sustained during bedrest. Following bedrest in the alendronate group, urinary calcium rose to 121 +/- 50 mg/day, a value less than that in the placebo group before or during bedrest. Similarly, urinary saturation of calcium oxalate and calcium phosphate rose with bedrest in the alendronate-treated patients but remained lower than values obtained in placebo-treated patients before or during bedrest. Alendronate inhibits bone mineral loss and averts the hypercalciuria and increased propensity for the crystallization of stone-forming calcium salts which occurs during 3 weeks of strict bedrest.

Non-NASA Center↗

Numerical Simulations of Buoyancy Effects in low Density Gas Jets

This paper deals with the computational analysis of buoyancy effects in the near field of an isothermal helium jet injected into quiescent ambient air environment. The transport equations of helium mass fraction coupled with the conservation equations of mixture mass and momentum were solved using a staggered grid finite volume method. Laminar, axisymmetric, unsteady flow conditions were considered for the analysis. An orthogonal system with non-uniform grids was used to capture the instability phenomena. Computations were performed for Earth gravity and during transition from Earth to different gravitational levels. The flow physics was described by simultaneous visualizations of velocity and concentration fields at Earth and microgravity conditions. Computed results were validated by comparing with experimental data substantiating that buoyancy induced global flow oscillations present in Earth gravity are absent in microgravity. The dependence of oscillation frequency and amplitude on gravitational forcing was presented to further quantify the buoyancy effects.

Satti, R. P.↗

MSFC Stream Model Preliminary Results: Modeling Recent Leonid and Perseid Encounters

The cometary meteoroid ejection model of Jones and Brown (1996b) was used to simulate ejection from comets 55P/Tempel-Tuttle during the last 12 revolutions, and the last 9 apparitions of 109P/Swift-Tuttle. Using cometary ephemerides generated by the Jet Propulsion Laboratory s (JPL) HORIZONS Solar System Data and Ephemeris Computation Service, two independent ejection schemes were simulated. In the first case, ejection was simulated in 1 hour time steps along the comet s orbit while it was within 2.5 AU of the Sun. In the second case, ejection was simulated to occur at the hour the comet reached perihelion. A 4th order variable step-size Runge-Kutta integrator was then used to integrate meteoroid position and velocity forward in time, accounting for the effects of radiation pressure, Poynting-Robertson drag, and the gravitational forces of the planets, which were computed using JPL s DE406 planetary ephemerides. An impact parameter was computed for each particle approaching the Earth to create a flux profile, and the results compared to observations of the 1998 and 1999 Leonid showers, and the 1993 and 2004 Perseids.

Cooke, William J.↗

Control of gravitropic orientation. II. Dual receptor model for gravitropism

Gravitropism of vascular plants has been assumed to require a single gravity receptor mechanism. However, based on the evidence in Part I of this study, we propose that maize roots require two. The first mechanism is without a directional effect and, by itself, cannot give rise to tropism. Its role is quantitative facilitation of the second mechanism, which is directional like the gravitational force itself and provides the impetus for tropic curvature. How closely coupled the two mechanisms may be is, as yet, unclear. The evidence for dual receptors supports a general model for roots. When readiness for gravifacilitation, or gravifacilitation itself, is constitutive, orthogravitropic curvature can go to completion. If not constitutively enabled, gravifacilitation can be weak in the absence of light and water deficit or strong in the presence of light and water deficit. In either case, it can decay and permit roots to assume reproducible non-vertical orientations (plagiogravitropic or plagiotropic orientations) without using non-vertical setpoints. In this way roots are deployed in a large volume of soil. Gravitropic behaviours in shoots are more diverse than in roots, utilising oblique and horizontal as well as vertical setpoints. As a guide to future experiments, we assess how constitutive v. non-constitutive modes of gravifacilitation might contribute to behaviours based on each kind of setpoint.

NASA Discipline Plant Biology↗

Evidence for Liquid Water on Comets

We have reexamined the arguments for the existence of liquid water on comets, and believe that recent cometary flybys along with pre-Giotto data support its presence on short-period comets. Liquid water would affect cometary dynamics, leaving distinct signatures in precession, orbital dynamics, and potential splitting of comets. Liquid water geysers would affect cometary atmosphere, dust evolution, and non-gravitational forces that perturb the orbit. Liquid water would affect the composition of both the interior and exterior of the comet, producing geologic effects consistent with recent flyby photographs. And most importantly, liquid water suppork the growth of lifeforms, which would make a comet a biofriendly incubator for interplanetary transport. The major objection against liquid water is the necessity of a pressure vessel to prevent sublimation into space. We discuss how such a pressure vessel could naturally evolve as a pristine comet makes its first journey inside the orbit of Mars, and suggest that this type of vessel was observed by Giotto, Deep Space I, and Stardust.

Sheldon, Robert↗

A Search for Meteor Shower Signatures in the LDEF IDE Data

For 346 days after the deployment of the LDEF satellite on April 7, 1984, the tape recorder belonging to the Interplanetary Dust Experiment (DE) stored information on over 15,000 impacts made by submicron and larger-size particles on its metal oxide silicon (MOS) detectors. These detectors were mounted on trays facing in six orthogonal directions - LDEF ram and trailing edge, the poles of the LDEF orbit (north and south), and radially inward (towards the Earth) and outward (towards space). The 13.1 second time resolution provided by the IDE electronics, combined with the high sensitivity of the MOS detectors and large collecting area (approximately 1 sq.m) of the experiment, conclusively showed that the small particle environment at the LDEF altitude of 480 km was highly time-variable, with particle fluxes spanning over four orders of magnitude. A large number of the 15,000 impacts recorded by IDE occurred in groups, which were of two types - the spikes, single, isolated events of high intensity and the multiple orbit event sequences (MOES), which were series of events separated in time by integer multiples of the LDEF orbital period. Even though the spikes were generally more intense, the MOES could be quite long-lived, some lasting for many days. A previous paper by Cooke et al. attributed the MOES to impacts by man-made debris particles in orbits intersecting that of LDEF. The 20 day longevity of one of these events - termed the May Swarm - led to the suggestion that the debris particles must be con- stantly replenished by their source, as the orbits of micron sized particles will rapidly decay under the influence of radiation pressure and other non-gravitational forces, entering Earth's atmosphere after only a few revolutions. However, the date of onset of the May Swarm (May 22) and the long duration of this event may indicate a possible correlation with the annual Arietid meteor shower, which peaks around June 8. As this seemed to hold the promise of a less artificial explanation than a satellite or rocket body continuously dribbling debris, it was decided to take a fresh look at parts of the IDE data set in an attempt to detect meteor showers within the impact record.

Cooke, William J.↗

Improving Robotic Operator Performance Using Augmented Reality

The Special Purpose Dexterous Manipulator (SPDM) is a two-armed robot that functions as an extension to the end effector of the Space Station Robotics Manipulator System (SSRMS), currently in use on the International Space Station (ISS). Crew training for the SPDM is accomplished using a robotic hardware simulator, which performs most of SPDM functions under normal static Earth gravitational forces. Both the simulator and SPDM are controlled from a standard robotic workstation using a laptop for the user interface and three monitors for camera views. Most operations anticipated for the SPDM involve the manipulation, insertion, and removal of any of several types of Orbital Replaceable Unit (ORU), modules which control various ISS functions. Alignment tolerances for insertion of the ORU into its receptacle are 0.25 inch and 0.5 degree from nominal values. The pre-insertion alignment task must be performed within these tolerances by using available video camera views of the intrinsic features of the ORU and receptacle, without special registration markings. Since optimum camera views may not be available, and dynamic orbital lighting conditions may limit periods of viewing, a successful ORU insertion operation may require an extended period of time. This study explored the feasibility of using augmented reality (AR) to assist SPDM operations. Geometric graphical symbols were overlaid on one of the workstation monitors to afford cues to assist the operator in attaining adequate pre-insertion ORU alignment. Twelve skilled subjects performed eight ORU insertion tasks using the simulator with and without the AR symbols in a repeated measures experimental design. Results indicated that using the AR symbols reduced pre-insertion alignment error for all subjects and reduced the time to complete pre-insertion alignment for most subjects.

Maida, James C.↗

Enhanced Lighting Techniques and Augmented Reality to Improve Human Task Performance

One of the most versatile tools designed for use on the International Space Station (ISS) is the Special Purpose Dexterous Manipulator (SPDM) robot. Operators for this system are trained at NASA Johnson Space Center (JSC) using a robotic simulator, the Dexterous Manipulator Trainer (DMT), which performs most SPDM functions under normal static Earth gravitational forces. The SPDM is controlled from a standard Robotic Workstation. A key feature of the SPDM and DMT is the Force/Moment Accommodation (FMA) system, which limits the contact forces and moments acting on the robot components, on its payload, an Orbital Replaceable Unit (ORU), and on the receptacle for the ORU. The FMA system helps to automatically alleviate any binding of the ORU as it is inserted or withdrawn from a receptacle, but it is limited in its correction capability. A successful ORU insertion generally requires that the reference axes of the ORU and receptacle be aligned to within approximately 0.25 inch and 0.5 degree of nominal values. The only guides available for the operator to achieve these alignment tolerances are views from any available video cameras. No special registration markings are provided on the ORU or receptacle, so the operator must use their intrinsic features in the video display to perform the pre-insertion alignment task. Since optimum camera views may not be available, and dynamic orbital lighting conditions may limit viewing periods, long times are anticipated for performing some ORU insertion or extraction operations. This study explored the feasibility of using augmented reality (AR) to assist with SPDM operations. Geometric graphical symbols were overlaid on the end effector (EE) camera view to afford cues to assist the operator in attaining adequate pre-insertion ORU alignment.

Maida, James C.↗

Navigation of the Deep Space 1 spacecraft at Borrelly

The navigation challenges posed by Deep Space 1's flyby of comet Borrelly were considerable due to the uncertainty in the knowledge of the comet's ephemeris, as well as difficulty in determining the spacecraft's ephemeris caused by relatively large non-gravitational forces acting on the comet. The challenges were met by using a combination of radio, optical, and interferometric data types to obtain a final fly by accuracy of less than 10 km.

comet encounter navigation autonomous navigation n↗

Rosetta Radio Science Investigations

The Rosetta Radio Science Investigations (RSI) experiment was selected by the European Space Agency to be included in the International Rosetta Mission to comet P/Wirtanen (launch in 2003, arrival and operational phase at the comet 2011-2013). The RSI science objectives address fundamental aspects of cometary physics such as the mass and bulk density of the nucleus, the gravity field, non-gravitational forces, the size and shape, the internal structure, the composition and roughness of the nucleus surface, the abundance of large dust grains and the plasma content in the coma and the combined dust and gas mass flux on the orbiter. RSI will make use of the radio system of the Rosetta spacecraft.

P/Wirtanen radio science cometary physics cometary↗

Constellations of elliptical inclined lunar orbits providing polar and global coverage

Prior results have developed a methodology for selecting a long-lived constellation of 3 satellites that provide persistent, stable coverage to either the North or South Pole with no requirement for stationkeeping under the influence of only gravitational perturbations. In the present study, the sensitivity of this coverage in the presence of non-gravitational forces is determined, and a design strategy is formulated that minimizes any potential sensitivity to these accelerations.

lunar constellation design↗

Electron Beam Freeform Fabrication in the Space Environment

The influence of reduced gravitational forces (in space and on the lunar or Martian surfaces) on manufacturing processes must be understood for effective fabrication and repair of structures and replacement parts during long duration space missions. The electron beam freeform fabrication (EBF3) process uses an electron beam and wire to fabricate metallic structures. The process efficiencies of the electron beam and the solid wire feedstock make the EBF3 process attractive for use in-space. This paper will describe the suitability of the EBF3 process in the space environment and will highlight preliminary testing of the EBF3 process in a zero-gravity environment.

Hafley, Robert A.↗

Journal of Gravitational Physiology, Volume 13, No. 1

At the outset, the Journal published one issue in 1994. The first number comprised the Proceedings of the 15th Annual International Gravitational Physiology Meeting, held in Barcelona, Spain in October 1993. The Proceedings of the previous 14 Annual Meetings appeared as supplements to The Physiologist from 1979 to 1993. Each year, one issue of the Journal is devoted to the Annual Meeting Proceedings, and up to four more issues are comprised of full-length research papers. Additionally, Supplement Issues are considered by the Editorial Board as they are submitted. The Journal is published for the International Society for Gravitational Physiology by the Galileo Foundation, a 501(c)(3) nonprofit public benefit corporation. This issue, the first number of 2006, comprises the Proceedings of the joint meeting of the International Society for Gravitational Physiology s 27th Annual International Gravitational Physiology Meeting, held in Osaka, Japan 23- 28 April, 2006. The Journal of Gravitational Physiology invites the submission of original experimental or observational papers on subjects in the field of gravitational physiology. Review articles, theoretical papers and historical or biographical articles will also be solicited by the Editor for publication. The wide scientific span of the Journal rests on physiology as its keystone. Gravitational physiology is considered to include the effects of changes in the magnitude and directions of the gravitational force environment on cells and physiological systems and behavior of humans, animals and plants. The effects of weightlessness during space flight, high sustained G forces and chronic acceleration, vibration, impact and the various forms of simulated weightlessness are also included, as well as is consideration of the evolutionary consequences of gravity and the role of gravity in the manifestation of scale effects in animals and plants.

Fuller, Charles A.↗

GRMHD and GRPIC Simulations

We have developed a new three-dimensional general relativistic magnetohydrodynamic (GRMHD) code by using a conservative, high-resolution shock-capturing scheme. The numerical fluxes are calculated using the HLL approximate Riemann solver scheme. The flux-interpolated constrained transport scheme is used to maintain a divergence-free magnetic field. We have performed various 1-dimensional test problems in both special and general relativity by using several reconstruction methods and found that the new 3D GRMHD code shows substantial improvements over our previous code. The simulation results show the jet formations from a geometrically thin accretion disk near a nonrotating and a rotating black hole. We will discuss the jet properties depended on the rotation of a black hole and the magnetic field configuration including issues for future research. A General Relativistic Particle-in-Cell Code (GRPIC) has been developed using the Kerr-Schild metric. The code includes kinetic effects, and is in accordance with GRMHD code. Since the gravitational force acting on particles is extreme near black holes, there are some difficulties in numerically describing these processes. The preliminary code consists of an accretion disk and free-falling corona. Results indicate that particles are ejected from the black hole. These results are consistent with other GRMHD simulations. The GRPIC simulation results will be presented, along with some remarks and future improvements. The emission is calculated from relativistic flows in black hole systems using a fully general relativistic radiative transfer formulation, with flow structures obtained by GRMHD simulations considering thermal free-free emission and thermal synchrotron emission. Bright filament-like features protrude (visually) from the accretion disk surface, which are enhancements of synchrotron emission where the magnetic field roughly aligns with the line-of-sight in the co-moving frame. The features move back and forth as the accretion flow evolves, but their visibility and morphology are robust. We would like to extend this research using GRPIC simulations and examine a possible new mechanism for certain X-ray quasi-periodic oscillations (QPOs) observed in blackhole X-ray binaries.

Nishikawa, K.-I.↗

Gravitational Effects upon Locomotion Posture

Researchers use actual microgravity (AM) during parabolic flight and simulated microgravity (SM) obtained with horizontal suspension analogs to better understand the effect of gravity upon gait. In both environments, the gravitational force is replaced by an external load (EL) that returns the subject to the treadmill. However, when compared to normal gravity (N), researchers consistently find reduced ground reaction forces (GRF) and subtle kinematic differences (Schaffner et al., 2005). On the International Space Station, the EL is applied by elastic bungees attached to a waist and shoulder harness. While bungees can provide EL approaching body weight (BW), their force-length characteristics coupled with vertical oscillations of the body during gait result in a variable load. However, during locomotion in N, the EL is consistently equal to 100% body weight. Comparisons between AM and N have shown that during running, GRF are decreased in AM (Schaffner et al, 2005). Kinematic evaluations in the past have focussed on joint range of motion rather than joint posture at specific instances of the gait cycle. The reduced GRF in microgravity may be a result of differing hip, knee, and ankle positions during contact. The purpose of this investigation was to compare joint angles of the lower extremities during walking and running in AM, SM, and N. We hypothesized that in AM and SM, joints would be more flexed at heel strike (HS), mid-stance (MS) and toe-off (TO) than in N.

DeWitt, John K.↗