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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 379 records · Page 21
All sourcesNASA NTRSDOE OSTI
NASA NTRS2003

A lignin-specific peroxidase in tobacco whose antisense suppression leads to vascular tissue modification

A tobacco peroxidase isoenzyme (TP60) was down-regulated in tobacco using an antisense strategy, this affording transformants with lignin reductions of up to 40-50% of wild type (control) plants. Significantly, both guaiacyl and syringyl levels decreased in essentially a linear manner with the reductions in lignin amounts, as determined by both thioacidolysis and nitrobenzene oxidative analyses. These data provisionally suggest that a feedback mechanism is operative in lignifying cells, which prevents build-up of monolignols should oxidative capacity for their subsequent metabolism be reduced. Prior to this study, the only known rate-limiting processes in the monolignol/lignin pathways involved that of Phe supply and the relative activities of cinnamate-4-hydroxylase/p-coumarate-3-hydroxylase, respectively. These transformants thus provide an additional experimental means in which to further dissect and delineate the factors involved in monolignol targeting to precise regions in the cell wall, and of subsequent lignin assembly. Interestingly, the lignin down-regulated tobacco phenotypes displayed no readily observable differences in overall growth and development profiles, although the vascular apparatus was modified.

NASA Program Fundamental Space Biology↗
NASA NTRS2002

Transcriptional control of monolignol biosynthesis in Pinus taeda: factors affecting monolignol ratios and carbon allocation in phenylpropanoid metabolism

Transcriptional profiling of the phenylpropanoid pathway in Pinus taeda cell suspension cultures was carried out using quantitative real time PCR analyses of all known genes involved in the biosynthesis of the two monolignols, p-coumaryl and coniferyl alcohols (lignin/lignan precursors). When the cells were transferred to a medium containing 8% sucrose and 20 mm potassium iodide, the monolignol/phenylpropanoid pathway was induced, and transcript levels for phenylalanine ammonia lyase, cinnamate 4-hydroxylase, p-coumarate 3-hydroxylase, 4-coumarate:CoA ligase, caffeoyl-CoA O-methyltransferase, cinnamoyl-CoA reductase, and cinnamyl alcohol dehydrogenase were coordinately up-regulated. Provision of increasing levels of exogenously supplied Phe to saturating levels (40 mm) to the induction medium resulted in further up-regulation of their transcript levels in the P. taeda cell cultures; this in turn was accompanied by considerable increases in both p-coumaryl and coniferyl alcohol formation and excretion. By contrast, transcript levels for both cinnamate 4-hydroxylase and p-coumarate 3-hydroxylase were only slightly up-regulated. These data, when considered together with metabolic profiling results and genetic manipulation of various plant species, reveal that carbon allocation to the pathway and its differential distribution into the two monolignols is controlled by Phe supply and differential modulation of cinnamate 4-hydroxylase and p-coumarate 3-hydroxylase activities, respectively. The coordinated up-regulation of phenylalanine ammonia lyase, 4-coumarate:CoA ligase, caffeoyl-CoA O-methyltransferase, cinnamoyl-CoA reductase and cinnamyl alcohol dehydrogenase in the presence of increasing concentrations of Phe also indicates that these steps are not truly rate-limiting, because they are modulated according to metabolic demand. Finally, the transcript profile of a putative acid/ester O-methyltransferase, proposed as an alternative catalyst for O-methylation leading to coniferyl alcohol, was not up-regulated under any of the conditions employed, suggesting that it is not, in fact, involved in monolignol biosynthesis.

NASA Discipline Plant Biology↗
NASA NTRS2000

Paleoproterozoic snowball earth: extreme climatic and geochemical global change and its biological consequences

Geological, geophysical, and geochemical data support a theory that Earth experienced several intervals of intense, global glaciation ("snowball Earth" conditions) during Precambrian time. This snowball model predicts that postglacial, greenhouse-induced warming would lead to the deposition of banded iron formations and cap carbonates. Although global glaciation would have drastically curtailed biological productivity, melting of the oceanic ice would also have induced a cyanobacterial bloom, leading to an oxygen spike in the euphotic zone and to the oxidative precipitation of iron and manganese. A Paleoproterozoic snowball Earth at 2.4 Giga-annum before present (Ga) immediately precedes the Kalahari Manganese Field in southern Africa, suggesting that this rapid and massive change in global climate was responsible for its deposition. As large quantities of O(2) are needed to precipitate this Mn, photosystem II and oxygen radical protection mechanisms must have evolved before 2.4 Ga. This geochemical event may have triggered a compensatory evolutionary branching in the Fe/Mn superoxide dismutase enzyme, providing a Paleoproterozoic calibration point for studies of molecular evolution.

Earth (Planet)↗
DOE OSTI2025

Meld: A project for exploring how to meet DUNE's framework needs

Existing data-processing frameworks for HEP experiments are largely based on collider-physics concepts, which may be based on rigid, event-based data hierarchies. These data organizations are not always helpful for neutrino experiments, which must sometimes work around such restrictions by manually splitting apart events into constructs that are better suited for neutrino physics. The purpose of Meld is to explore more flexible data organizations by treating a frameworks job as: (1) A graph of data-product sequences connected by (2) User-defined functions that serve as operations to (3) Framework-provided higher-order functions.

Knoepfel, KyleJ. [Fermi National Accelerator Labor↗
DOE OSTI2024

NovaDemux v39.07

This program is a sequence demultiplexer intended primarily for, but not limited to, Illumina sequencing machines. Typically, multiple experiments ("libraries") are pooled together and sequenced at once, with genetic molecules of these libraries tagged with a synthetic DNA "barcode". After sequencing, the data is demultiplexed into one file per library based on the barcode. However, errors in barcode reading cause misassignment and decrease yield. NovaDemux uses advanced statistical methods to maximize yield while minimizing misassignment compared to existing software.

Bushnell, Brian [Lawrence Berkeley National Labora↗
DOE OSTI2024

Protein N -Glycans in Healthy and Sclerotic Glomeruli in Diabetic Kidney Disease

Diabetes is expected to directly affect renal glycosylation; yet to date, there has not been a comprehensive evaluation of alterations in N-glycan composition in the glomeruli of patients with diabetic kidney disease (DKD). Here, we used untargeted mass spectrometry imaging to identify N-glycan structures in healthy and sclerotic glomeruli in formalin-fixed paraffin-embedded sections from needle biopsies of five patients with DKD and three healthy kidney samples. Regional proteomics was performed on glomeruli from additional biopsies from the same patients to compare the abundances of enzymes involved in glycosylation. Secondary analysis of single-nucleus RNA sequencing (snRNAseq) data were used to inform on transcript levels of glycosylation machinery in different cell types and states. We detected 120 N-glycans, and among them, we identified 12 of these protein post-translated modifications that were significantly increased in glomeruli. All glomeruli-specific N-glycans contained an N-acetyllactosamine epitope. Five N-glycan structures were highly discriminant between sclerotic and healthy glomeruli. Sclerotic glomeruli had an additional set of glycans lacking fucose linked to their core, and they did not show tetra-antennary structures that were common in healthy glomeruli. Orthogonal omics analyses revealed lower protein abundance and lower gene expression involved in synthesizing fucosylated and branched N-glycans in sclerotic podocytes. In snRNAseq and regional proteomics analyses, we observed that genes and/or proteins involved in sialylation and N-acetyllactosamine synthesis were also downregulated in DKD glomeruli, but this alteration remained undetectable by our spatial N-glycomics assay. Integrative spatial glycomics, proteomics, and transcriptomics revealed protein N-glycosylation characteristic of sclerotic glomeruli in DKD.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2025

Phylodynamics of SARS-CoV-2 Lineages B.1.1.7, B.1.1.529 and B.1.617.2 in Nigeria Suggests Divergent Evolutionary Trajectories

Background: The early months of the COVID-19 pandemic were characterized by high transmission rates and mortality, compounded by the emergence of multiple SARS-CoV-2 lineages, including Variants of Concern (VOCs). This study investigates the phylodynamic and spatio-temporal trends of VOCs during the peak of the pandemic in Nigeria. Methods: Whole-genome sequencing (WGS) data from three major VOCs circulating in Nigeria, B.1.1.7 (Alpha), B.1.617.2 (Delta), and B.1.1.529 (Omicron), were analyzed using tools such as Nextclade, R Studio v 4.2.3, and BEAST X v 10.5.0. The spatial distribution, evolutionary history, viral ancestral introductions, and geographic dispersal patterns were characterized. Results: Three major lineages following WHO nomenclature were identified: Alpha, Delta, and Omicron. The Delta variant exhibited the widest geographic spread, detected in 14 states, while the Alpha variant was the least distributed, identified in only eight states but present across most epidemiological weeks studied. Evolutionary rates varied slightly, with Alpha exhibiting the slowest rate (2.66 × 10 −4 substitutions/site/year). Viral population analyses showed distinct patterns: Omicron sustained elevated population growth over time, while Delta declined after initial expansion. The earliest Times to Most Recent Common Ancestor (TMRCA) were consistent with the earliest outbreaks of SARS-CoV-2 globally. Geographic transmission analysis indicated a predominant coastal-to-inland spread for all variants, with Omicron showing the most diffuse dispersal, highlighting commercial routes as significant drivers of viral diffusion. Conclusion: The SARS-CoV-2 epidemic in Nigeria was characterized by multiple variant introductions and a dominant coastal-to-inland spread, emphasizing that despite lockdown measures, commercial trade routes played a critical role in viral dissemination. These findings provide insights into pandemic control strategies and future outbreak preparedness.

Nigeria↗
NASA NTRS1975

The detection of tightly closed flaws by nondestructive testing (NDT) methods

Liquid penetrant, ultrasonic, eddy current and X-radiographic techniques were optimized and applied to the evaluation of 2219-T87 aluminum alloy test specimens in integrally stiffened panel, and weld panel configurations. Fatigue cracks in integrally stiffened panels, lack-of-fusion in weld panels, and fatigue cracks in weld panels were the flaw types used for evaluation. A 2319 aluminum alloy weld filler rod was used for all welding to produce the test specimens. Forty seven integrally stiffened panels containing a total of 146 fatigue cracks, ninety three lack-of-penetration (LOP) specimens containing a total of 239 LOP flaws, and one-hundred seventeen welded specimens containing a total of 293 fatigue cracks were evaluated. Nondestructive test detection reliability enhancement was evaluated during separate inspection sequences in the specimens in the 'as-machined or as-welded', post etched and post proof loaded conditions. Results of the nondestructive test evaluations were compared to the actual flaw size obtained by measurement of the fracture specimens after completing all inspection sequences. Inspection data were then analyzed to provide a statistical basis for determining the flaw detection reliability.

Rummel, W. D.↗
NASA NTRS1976

Surface-pressure fluctuations associated with aerodynamic noise on the space shuttle launch configuration at transonic and supersonic speeds

A 0.035-scale space shuttle model was recently tested at Ames Research Center to define the fluctuating surface pressures during the launch phase of the full-scale vehicle. A 120-channel data acquisition and reduction system was employed to obtain fluctuating pressure measurements from 240 pressure transducers in two separate data passes. Time histories from all transducers were recorded on four 32-track FM tape recorders. A minicomputer was used to compute and record on-line rms values, the fluctuating pressure coefficient, and full-scale dB levels. The minicomputer also partially controlled the test conditions and fully controlled all data acquisition sequences, thus greatly improving the efficiency of test operations. This paper presents details of the tests and illustrates the fluctuating pressures that will occur on the space shuttle and launch vehicle.

Hanly, R. D.↗
NASA NTRS1977

ALTKAL: An optimum linear filter for GEOS-3 altimeter data

ALTKAL is a computer program designed to smooth sea surface height data obtained from the GEOS 3 altimeter, and to produce minimum variance estimates of sea surface height and sea surface slopes, along with their standard derivations. The program operates by processing the data through a Kalman filter in both the forward and backward directions, and optimally combining the results. The sea surface height signal is considered to have a geoid signal, modeled by a third order Gauss-Markov process, corrupted by additive white noise. The governing parameters for the signal and noise processes are the signal correlation length and the signal-to-noise ratio. Mathematical derivations of the filtering and smoothing algorithms are presented. The smoother characteristics are illustrated by giving the frequency response, the data weighting sequence and the transfer function of a realistic steady-state smoother example. Based on nominal estimates for geoidal undulation amplitude and correlation length, standard deviations for the estimated sea surface height and slope are 12 cm and 3 arc seconds, respectively.

Fang, B. T.↗
NASA NTRS1977

Evolution of helium stars

The evolution of helium stars in the mass range from 4 to 15 solar masses has been followed from the initial helium main sequence to the end of carbon burning in the core, with the use of Carson's (1976) radiative opacities. As compared with earlier work based on smaller opacities, the main-sequence band in the H-R diagram is now both wider and cooler than before. If neutrino losses are neglected in the stellar models, the phase of carbon burning in the core occurs in the red-supergiant region; otherwise, it occurs, as it does in the earlier models with or without neutrino emission, close to the helium main sequence. Observational data for Wolf-Rayet stars and R Coronae Borealis variables are found to lend some support to the present models.

Stothers, R.↗
NASA NTRS1978

Algorithms for isolating worst case systematic data errors

Two separate algorithms are derived for testing filter sensitivity to systematic data errors. One algorithm provides the absolute minimum Euclidean norm data error for a given estimate component error. The second algorithm can be used to find the minimum norm data error which can be generated by restricted degree Legendre polynomials. A specific very long baseline interferometry (VLBI) baseline estimation is analyzed with the algorithm. It is found that the local vertical is the most sensitive component to error in the data space. The efficiency of a data error sequence linear in elevation angle is within 7% that of the absolute worst case sequence. Elevation angle dependent errors are explored and the special case of a mismodeled troposphere is treated.

Curkendall, D. W.↗
NASA NTRS1978

Transverse flat plate heat pipe experiment

This paper describes a Shuttle-launched flight experiment to evaluate the performance of a transverse flat plate heat pipe that serves as an integral temperature control/mounting panel for electronic equipment. A transverse heat pipe is a gas-controlled variable conductance heat pipe that can handle relatively large thermal loads. An experiment designed to flight test the concept over a 6-9 month period is self-sufficient with respect to electrical power, timing sequences, and data storage.

Edelstein, F.↗
NASA NTRS1978

Chemical condensation sequences in supernova ejecta

Equilibrium condensation is investigated for composition and pressures representative of supernova (SN) ejecta. The results are compared with those obtained for the solar nebula, and the distribution of elements among the various gaseous species and solid phases is computed for solar and SN-shell condensation sequences. The data are applied to problems concerning the observed mass fractionations of Si, Mg, and O in Allende, as well as to the strong depletion of heavy-element abundances in the interstellar medium. The results indicate that dust grains from a SN could have introduced isotopic anomalies into the solar nebula, that many minerals produced in SN ejecta are the same as those predicted to be stable in the solar nebula, and that the observed depletion of heavy elements in the interstellar gas could be due to dust formation in SN ejecta.

Lattimer, J. M.↗
NASA NTRS1978

The Space Shuttle Ground Terminal Delta Modulation System

Shuttle voice processing uses a Variable Slope Delta Modulation scheme which is a variant of the ABATE algorithm. This paper summarizes the ground terminal subsystem which processes the multiplexed voice and telemetry data digital sequence in the downlink processing and digitizes the voice signal in the uplink processing. The Delta Modulation System (DMS) performance characteristics are given in terms of an intelligibility test score and in terms of time and frequency responses measured on a production system developed for NASA Goddard Space Flight Center. Implementation details of the encoder and decoder algorithms are given in terms of the circuit logic used to implement them.

Auger, R. L.↗
NASA NTRS1979

Formation, history and energetics of cores in the terrestrial planets

The size, evolution and energetics of the earth's core and the probable central, metalic cores of Mercury, Venus, the moon and Mars are discussed. The cores of Mercury, Mars and the earth are considered likely to decrease in relative mass and volume with distance from the sun; the moon does not fit this sequence and data from Venus are insufficient. Core formation is concluded to have occurred early (prior to four billion years ago) on the earth and Mercury, while that on the moon would have occurred over a longer interval and the core of Mars would have formed much later. Of the possible energy sources in planetary cores able to maintain a molten state and drive magnetic dynamos, the energy of core formation was probably spent too early in planetary history to provide much present power, and the energy gained from freezing an inner core in the earth and Mercury are considered at best marginally able to match conductive heat loss. Future measurements proposed to improve the quantitative understanding of core properties include a better documentation of the magnetic fields of Venus and Mars and the seismometry of Mars.

Solomon, S. C.↗