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The Highest L(sub X)/L(sub opt) Sources in the ROSAT All-Sky Survey

The purpose of our program was to identify new and interesting soft X-ray sources from the Bright Source Catalog of the ROSAT All-Sky Survey. Our intent was to use XMM to observe a sample of BSC objects that had been identified as less than 10% likely to be associated with any object in the USNO-A2.0 catalog (Rutledge et al. 2000). We requested a single 5-ks pointing for each of 32 sources in this category in order to make a systematic examination of the properties of these sources. Ultimately, we hoped to identify new isolated neutron stars from within this population. Our requested observations were scheduled for execution at the end of Cycle 2, for two of our targets only (selected by sky position). A two-target sample does not allow for the statistical investigation that we originally proposed; however, based on the identification of the BSC object in the XMM data, an improved position, and reevaluation of likely off-band counterparts, it does enable a source-by-source evaluation of whether the BSC object is an isolated neutron star (INS).

Kulkarni, Shrinivas R.↗

Osiris-REx Spacecraft Current Status and Forward Plans

The NASA New Frontiers OSIRIS-REx spacecraft executed a flawless launch on September 8, 2016 to begin its 23-month journey to near-Earth asteroid (101955). The primary objective of the OSIRIS-REx mission is to collect and return to Earth a pristine sample of regolith from the asteroid surface. The sampling event will occur after a two-year period of remote sensing that will ensure a high probability of successful sampling of a region on the asteroid surface having high science value and within well-defined geological context. The OSIRIS-REx instrument payload includes three high-resolution cameras (OCAMS), a visible and near-infrared spectrometer (OVIRS), a thermal imaging spectrometer (OTES), an X-ray imaging spectrometer (REXIS), and a laser altimeter (OLA). As the spacecraft follows its nominal outbound-cruise trajectory, the propulsion, power, communications, and science instruments have undergone basic functional tests, with no major issues. Outbound cruise science investigations include a search for Earth Trojan asteroids as the spacecraft approaches the Sun-Earth L4 Lagrangian point in February 2017. Additional instrument checkouts and calibrations will be carried out during the Earth gravity assist maneuver in September 2017. During the Earth-moon flyby, visual and spectral images will be acquired to validate instrument command sequences planned for Bennu remote sensing. The asteroid Bennu remote sensing campaign will yield high resolution maps of the temperature and thermal inertia, distributions of major minerals and concentrations of organic matter across the asteroid surface. A high resolution 3d shape model including local surface slopes and a high-resolution gravity field will also be determined. Together, these data will be used to generate four separate maps that will be used to select the sampling site(s). The Safety map will identify hazardous and safe operational regions on the asteroid surface. The Deliverability map will quantify the accuracy with which the navigation team can deliver the spacecraft to and from specific sites on the asteroid surface. The Sampleability map quantifies the regolith properties, providing an estimation of how much material would be sampled at different points on the surface. The final Science Value map synthesizes the chemical, mineralogical, and geological, observations to identify the areas of the asteroid surface with the highest science value. Here, priority is given to organic, water-rich regions that have been minimally altered by surface processes. Asteroid surface samples will be acquired with a touch-and-go sample acquisition system (TAGSAM) that uses high purity pressurized N2 gas to mobilize regolith into a stainless steel canister. Although the mission requirement is to collect at least 60 g of material, tests of the TAGSAM routinely exceeded 300 g of simulant in micro-gravity tests. After acquiring the sample, the spacecraft will depart Bennu in 2021 to begin its return journey, with the sample return capsule landing at the Utah Test and Training Range on September 23, 2023. The OSIRIS-REx science team will carry out a series of detailed chemical, mineralogical, isotopic, and spectral studies that will be used to determine the origin and history of Bennu and to relate high spatial resolution sample studies to the global geological context from remote sensing. The outline of the sample analysis plan is described in a companion abstract.

Messenger, Scott↗

Spatial variation of primordial 3-He in crustal fluids along the East-African Rift system (the Ethiopian and the Kenya Rift section)

(3)He/(4)He compositions are presented for groundwater samples from the Ethiopian segment of the East-Afrikan Rift and from its northern extension, the adjacent Afar region (Djibuti). Helium isotope data are compared to those obtained previously from the Gregory Rift, south of Ethiopia. The distribution pattern of mantle-derived volatiles along the entire East-African-Rift (-from south Kenya to Djibuti-) is discussed and their sources are identified. Helium isotope ratios (R) for samples from the Ethiopian part of the Rift range from 6.3 to 16.0 times the atmospheric ratio (Ra=1.4 x 10(exp -6) and thus show together with a MOR component a considerable hotspot helium component. These mantle helium concentrations are comparable to those observed in groundwaters and volcanic rocks from the Afar plume region in Djibuti. Here R/Ra values range from 9 to 13 times the atmospheric composition, with mantle-derived helium concentrations being higher than at spreading ocean ridges. R/Ra values from Ethiopia and Djibuti are entirely different from those observed in groundwaters at the southerly extending Gregory Rift in Kenya, where R/Ra values scatter between 0.5 and 6. At the northernmost part of the Gregory Rift, close to Ethiopia mantle helium contents are slightly higher, with R/Ra-values varying between 6.5 and 8.0.

Griesshaber, E.↗

Identifying Aerosol Type/Mixture from Aerosol Absorption Properties Using AERONET

Aerosols are generated in the atmosphere through anthropogenic and natural mechanisms. These sources have signatures in the aerosol optical and microphysical properties that can be used to identify the aerosol type/mixture. Spectral aerosol absorption information (absorption Angstrom exponent; AAE) used in conjunction with the particle size parameterization (extinction Angstrom exponent; EAE) can only identify the dominant absorbing aerosol type in the sample volume (e.g., black carbon vs. iron oxides in dust). This AAE/EAE relationship can be expanded to also identify non-absorbing aerosol types/mixtures by applying an absorption weighting. This new relationship provides improved aerosol type distinction when the magnitude of absorption is not equal (e.g, black carbon vs. sulfates). The Aerosol Robotic Network (AERONET) data provide spectral aerosol optical depth and single scattering albedo - key parameters used to determine EAE and AAE. The proposed aerosol type/mixture relationship is demonstrated using the long-term data archive acquired at AERONET sites within various source regions. The preliminary analysis has found that dust, sulfate, organic carbon, and black carbon aerosol types/mixtures can be determined from this AAE/EAE relationship when applying the absorption weighting for each available wavelength (Le., 440, 675, 870nm). Large, non-spherical dust particles absorb in the shorter wavelengths and the application of 440nm wavelength absorption weighting produced the best particle type definition. Sulfate particles scatter light efficiently and organic carbon particles are small near the source and aggregate over time to form larger less absorbing particles. Both sulfates and organic carbon showed generally better definition using the 870nm wavelength absorption weighting. Black carbon generation results from varying combustion rates from a number of sources including industrial processes and biomass burning. Cases with primarily black carbon showed improved definition in the 870nm wavelength absorption weighting due to the increased absorption in the near-infrared wavelengths, while the 440nm wavelength provided better definition when black carbon mixed with dust. Utilization of this particle type scheme provides necessary information for remote sensing applications, which needs a priori knowledge of aerosol type to model the retrieved properties especially over semi-bright surfaces. In fact, this analysis reveals that the aerosol types occurred in mixtures with varying magnitudes of absorption and requires the use of more than one assumed aerosol mixture model. Furthermore, this technique will provide the aerosol transport model community a data set for validating aerosol type.

Giles, D. M.↗

Green icebergs formed by freezing of organic-rich seawater to the base of Antarctic ice shelves

Samples of Antarctic seawater, basal ice, and green ice from ice cliffs and green icebergs are analyzed in order to examine green icebergs formed by the freezing of organic-rich seawater to the base of Antarctic ice shelves. Spectral reflectance of a green iceberg measured near 67 deg S, 62 deg E confirms that the color is inherent in the ice, not an artifact of the illumination. A constituent that absorbs blue photons is identified by spectrophotometric analysis of core samples from this iceberg and from the Amery basal ice, and of seawater samples from Prydz Bay off the Amery Ice Shelf. Analysis of the samples by fluorescence spectroscopy indicates that the blue absorption, and hence the inherent green color, is due to the presence of marine-derived organic matter in the green iceberg, basal ice, and seawater. Thick accumulations of green ice, in icebergs, and at the base of ice shelves indicate that high concentrations of organic matter exist in seawater for centuries at the depth of basal freezing.

Warren, Stephen G.↗

Radio properties of extragalactic IRAS sources

The present study identifies extragalactic sources from the IRAS Faint Source Catalog by position coincidence with radio sources stronger than 25 mJy and lying north of +5 deg on the Green Bank 4.85 GHz sky maps. Published VLA maps, new 4.86 GHz VLA maps made with 15-arcsec resolution, and accurate optical positions are used to confirm 122 of these candidate identifications. Normal and starburst spiral galaxies were found to comprise about 97 percent of the FIR flux-limited sample. Radio-loud 'monsters' with q less than 2.25 dominate the radio emission from about 2 percent of the FIR source sample, and radio-quiet monsters are responsible for the FIR emission from less than about 1 percent of the FIR sample. All of the radio-identified sources are optically identified, mostly with relatively bright nearby galaxies. No evidence was found for any new populations of high-redshift FIR sources, nonthermal sources with steep FIR/optical spectra, or dust-shrouded sources visible only at FIR and radio wavelengths.

Condon, J. J.↗

Eyjafjallajokull Volcano Plume Particle-Type Characterization from Space-Based Multi-angle Imaging

The Multi-angle Imaging SpectroRadiometer (MISR) Research Aerosol algorithm makes it possible to study individual aerosol plumes in considerable detail. From the MISR data for two optically thick, near-source plumes from the spring 2010 eruption of the Eyjafjallaj kull volcano, we map aerosol optical depth (AOD) gradients and changing aerosol particle types with this algorithm; several days downwind, we identify the occurrence of volcanic ash particles and retrieve AOD, demonstrating the extent and the limits of ash detection and mapping capability with the multi-angle, multi-spectral imaging data. Retrieved volcanic plume AOD and particle microphysical properties are distinct from background values near-source, as well as for overwater cases several days downwind. The results also provide some indication that as they evolve, plume particles brighten, and average particle size decreases. Such detailed mapping offers context for suborbital plume observations having much more limited sampling. The MISR Standard aerosol product identified similar trends in plume properties as the Research algorithm, though with much smaller differences compared to background, and it does not resolve plume structure. Better optical analogs of non-spherical volcanic ash, and coincident suborbital data to validate the satellite retrieval results, are the factors most important for further advancing the remote sensing of volcanic ash plumes from space.

Kahn, Ralph A.↗

Searching for Crisium Basin ejecta - Chemistry and ages of Luna 20 impact melts

Chemical (INAA) and chronological (Ar-40 - Ar-39) analyses of six Luna 20 impact melts are performed, and these are compared to the results of Podosek et al. (1973), commonly taken to be representative of the Crisium Basin impact. At least two chemical groups of impact melts are identified. One is interpreted as melts derived from the local crust by craters over the last 3.9 Ga. Another group, which includes one of the samples dated by Podosek et al. (1973), is chemically and chronologically (3.85 + or - 0.02 Ga) indistinguishable from Apollo 17 samples interpreted as Serenitatis impact melts, and hence could be melt formed by that event and deposited at the Luna 20 site. One sample (22023,3,F) has a well-defined age of 3.895 + or - 0.017 Ga, and is chemically similar to, but distinct from, impact melts from other basins. This sample, and its chemistry and age are tentatively identified with the Crisium Basin impact. Whether the age of the Crisium impact is given by 22023,3,F, the samples analyzed by Podosek et al. (1973), or neither, it is clear that Crisium impact melt is not abundant in the Luna 20 collection.

Swindle, T. D.↗

Laboratory Studies of Fischer-Tropsch-Type Reactions and Their Implications for Organics in Asteroids and Comets

We have been studying Fischer-Tropsch type (FTT) reactions as a source for organic materials both in the gas phase of the solar nebula and incorporated into primitive comets and asteroids for almost 10 years, and over this time our concept has evolved greatly from the standard "catalytic" model to a much more robust chemical scenario. Our simulations have been conducted at temperatures that are much higher than we like, primarily for practical reasons such as the timescale of individual reactions, and we are just starting a series of measurements to allow us to measure reaction rates at temperatures from 873K down to as low as 373K. We have preliminary data on the carbon (d13C = -50) & nitrogen (d15N = +9.5) isotopic fractionation at 873K, but not on materials produced at lower temperature. Isotope values are on the VPDB scale for carbon and vs. Air for nitrogen. We have also investigated the noble gas trapping efficiency of the FTT process by adding a small amount of a noble gas mix to our standard synthesis mix. The noble gas ratio is 49:49:1:1::Ne:Ar:Kr:Xe. Xe and Kr are trapped at 873K and are more efficiently trapped at 673K with no isotopic fractionation at either temperature. Ar trapping is detected at 673K, but not at 873K. Ne has not yet been observed in our samples. The solar nebula was an extremely complex system, mixing materials from the innermost regions out to well into the zones where comets formed and thus mixing highly processed nebular materials with grains and coatings formed before the nebula began to collapse. Laboratory studies may provide the means to separate such diverse components based on carbon or nitrogen isotopic fractionation or the quantities of noble gases trapped in grain coatings and their thermal release patterns, among other observables. The ultimate goal of laboratory synthesis of nebular analogs is to provide the means to identifY the conditions under which natural samples were formed and the signatures of subsequent metamorphic events.

Nuth, Joseph↗

[EC5-Space Suit Assembly Team- Internship]

There were three main projects in this internship. The first pertained to the Bearing Dust Cycle Test, in particular automating the test to allow for easier administration. The second concerned modifying the communication system setup in the Z2 suit, where speakers and mics were adjusted to allow for more space in the helmet. And finally, the last project concerned the tensile strength testing of fabrics deemed as candidates for space suit materials and desired to be sent off for radiation testing. The major duties here are split up between the major projects detailed above. For the Bearing Dust Cycle Test, the first objective was to find a way to automate administration of the test, as the previous version was long and tedious to perform. In order to do this, it was necessary to introduce additional electronics and perform programming to control the automation. Once this was done, it would be necessary to update documents concerning the test setup, procedure, and potential hazards. Finally, I was tasked with running tests using the new system to confirm system performance. For the Z2 communication system modifications, it was necessary to investigate alternative speakers and microphones which may have better performance than those currently used in the suit. Further, new speaker and microphone positions needed to be identified to keep them out of the way of the suit user. Once this was done, appropriate hardware (such as speaker or microphone cases and holders) could be prototyped and fabricated. For the suit material strength testing, the first task was to gather and document various test fabrics to identify the best suit material candidates. Then, it was needed to prepare samples for testing to establish baseline measurements and specify a testing procedure. Once the data was fully collected, additional test samples would be prepared and sent off-site to undergo irradiation before being tested again to observe changes in strength performance. For the Bearing Dust Cycle Test, automation was achieved through use of a servo motor and code written in LabVIEW. With this a small electrical servo controller was constructed and added to the system. For the Z2 communication modifications speaker cases were developed and printed, and new speakers and mics were selected. This allowed us to move the speakers and mics to locations to remain out of the suit users way. For the suit material strength testing, five material candidates were identified and test samples were created. These samples underwent testing, and baseline test results were gathered, though these results are currently being investigated for accuracy. The main process efficiency developed during the course of this internship comes from automation of the Bearing Dust Cycle Test. In particular, many hours of human involvement and precise operation are replaced with a simple motor setup. Thus it is no longer required to man the test, saving valuable employee time. This internship has confirmed a few things for me, namely that I both want to work as an engineer for an aerospace firm and that in particular I want to work for the Johnson Space Center. I am also confirmed in my desire to work with electronics, though I was surprised to enjoy prototyping 3D CAD design as much as I did. Therefore, I will make an effort to build my skills in this area so that I can continue to design mechanical models. In fact, I found the process of hands-on prototyping to be perhaps the most fun aspect of my time working here. This internship has also furthered my excitement for continual education, and I will hopefully be pursuing a masters in my field in the near future.

Maicke, Andrew↗

Petrographic and petrological studies of lunar rocks

Clasts, rind glass, matrix glass, and matrix minerals from five Apollo 15 glass-coated breccias (15255, 15286, 15465, 15466, and 15505) were studied optically and with the SEM/microprobe. Rind glass compositions differ from sample to sample, but are identical, or nearly so, to the local soil, suggesting their origin by fusion of that soil. Most breccia samples contain green or colorless glass spheres identical to the Apollo 15 green glasses. These glasses, along with other glass shards and fragments, indicate a large soil component is present in the breccias. Clast populations include basalts and gabbros containing phases highly enriched in iron, indicative of extreme differentiation or fractional crystallization. Impact melts, anorthosites, and minor amounts of ANT suite material are also present among the clasts. Tektite glasses, impact melts, and breccias from the Zhamanshin structure, USSR, were also studied. Basic tektite glasses were found to be identical in composition to impact melts from the structure, but no satisfactory parent material has been identified in the limited suite of samples available.

Winzer, S. R.↗

Cognitive Performance in ISS Astronauts on 6-Month Low Earth Orbit Missions

Introduction: Current and future astronauts will endure prolonged exposure to spaceflight hazards and environmental stressors that could compromise cognitive functioning, yet cognitive performance in current missions to the International Space Station remains critically under-characterized. We systematically assessed cognitive performance across 10 cognitive domains in astronauts on 6-month missions to the ISS. Methods: Twenty-five professional astronauts were administered the Cognition Battery as part of National Aeronautics and Space Administration (NASA) Human Research Program Standard Measures Cross-Cutting Project. Cognitive performance data were collected at five mission phases: pre-flight, early flight, late flight, early post-flight, and late post-flight. We calculated speed and accuracy scores, corrected for practice effects, and derived z-scores to represent deviations in cognitive performance across mission phases from the sample’s mean baseline (i.e., pre-flight) performance. Linear mixed models with random subject intercepts and pairwise comparisons examined the relationships between mission phase and cognitive performance. Results: Cognitive performance was generally stable over time with some differences observed across mission phases for specific subtests. There was slowed performance observed in early flight on tasks of processing speed, visual working memory, and sustained attention. We observed a decrease in risk-taking propensity during late flight and post-flight mission phases. Beyond examining group differences, we inspected scores that represented a significant shift from the sample’s mean baseline score, revealing that 11.8% of all flight and post-flight scores were at or below 1.5 standard deviations below the sample’s baseline mean. Finally, exploratory analyses yielded no clear pattern of associations between cognitive performance and either sleep or ratings of alertness. Conclusions: There was no evidence for a systematic decline in cognitive performance for astronauts on a 6-month missions to the ISS. Some differences were observed for specific subtests at specific mission phases, suggesting that processing speed, visual working memory, sustained attention, and risk-taking propensity may be the cognitive domains most susceptible to change in Low Earth Orbit for high performing, professional astronauts. We provide descriptive statistics of pre-flight cognitive performance from 25 astronauts, the largest published preliminary normative database of its kind to date, to help identify significant performance decrements in future samples.

Data curation↗

Microbial Air and Surface Monitoring Results from International Space Station Samples

Over the course of long-duration spaceflight, spacecraft develop a microbial ecology that directly interacts with the crew of the vehicle. While most microorganisms are harmless or beneficial to the inhabitants of the vehicle, the presence of medically significant organisms appearing in this semi-closed environment could adversely affect crew health and performance. The risk of exposure of the crew to medically significant organisms during a mission is estimated using information gathered during nominal and contingency environmental monitoring. Analysis of the air and surface microbiota in the habitable compartments of the International Space Station (ISS) over the last four years indicate a high presence of Staphylococcus species reflecting the human inhabitants of the vehicle. Generally, air and surface microbial concentrations are below system design specifications, suggesting a lower risk of contact infection or biodegradation. An evaluation of sample frequency indicates a decrease in the identification of new species, suggesting a lower potential for unknown microorganisms to be identified. However, the opportunistic pathogen, Staphylococcus aureus, has been identified in 3 of the last 5 air samples and 5 of the last 9 surface samples. In addition, 47% of the coagulase negative Staphylococcus species that were isolated from the crew, ISS, and its hardware were found to be methicillin resistance. In combination, these observations suggest the potential of methicillin resistant infectious agents over time.

Ott, C. Mark↗

Identification of Primitive Unequilibrated Ordinary Chondrites from Four Antarctic Dense Collections Areas (EET, GRO, LEW, and MET)

Unequilibrated ordinary chondrites (UOC) typically contain metal [1], primitive chondrules [2], pre-solar grains [3], organics [4], nebular materials in matrix [5], and isotopic records of early solar system processes [6], and are thus scientifically valuable samples that provide important information about the early solar system. UOCs from dense collection areas (DCA) like in the Transantarctic Mtns. pose several specific challenges. First, they are commonly part of large pairing groups for which preliminary studies are followed up by detailed studies that reveal differences among the pairs (e.g., [7,8]). Second, subsequent visits to the DCA identify and recover new members of the pairing group. Thus over time, the pairing groups must be re-assessed as new information becomes available for existing and new members of the larger pairing groups. Given the suggestion that many samples in these groups could be more primitive than L3.4 (e.g., [7]), we have undertaken a survey of 103 chondrites. Here we report olivine analyses from Type II (FeO-rich) chondrules with a goal of identifying primitive (<L3.20) UOCs using the Cr in olivine approach [9]. In the process of the detailed work, we identified 22 samples of petrologic grade 3.2 or lower that were previously unrecognized. Pairing relations of L3.x chondrites in these 3 areas are accordingly re-assessed.

chondrite↗

The Scientific Value of Collecting Samples From the Jezero Crater Rim

The Mars 2020 mission has been conducting ground-based investigation of the geology, habitability, and biosignature preservation potential and collecting samples for return to Earth in Jezero crater, Mars for nearly 3 years. Analysis of these samples will address outstanding questions in Mars science including potential habitability and how and why the climate the interior of the planet evolved through time. As of December 2023, samples of 4 igneous rocks of the Jezero crater floor and 9 sedimentary rocks of the Jezero fan and inner margin, remain on the rover. 15 tubes remain to be filled to enhance the diversity of the cache and broaden the scope of the science questions that can be addressed with returned sample studies. The next step in the mission is to explore the Jezero crater rim. It will be imperative to investigate and sample the diversity of crater rim rocks because they represent materials from Mars’ most ancient crust older than those sampled in Jezero crater, a diversity of geologic processes, and potential ancient habitable environments that have not yet been investigated or sampled. Ongoing mapping efforts are using orbiter data and long-distance images from Perseverance to identify and interpret the geologic context of the crater rim. Building on this effort and the broader geologic context for the crater rim put forward by previous studies, we identify diverse targets for in situ investigation and potential sampling by Mars 2020.

Mars sample return↗

The ultraviolet absorption spectrum of the quasar PKS 0405-12 and the local density of Lyman-alpha absorption systems

A sample of 32 absorption lines has been identified in the ultraviolet spectrum of the z = 0.57 quasar PKS 0405-12. Data cover the wavelength range 1190-3260 A. There are 10 extragalactic Ly-alpha absorption lines in the complete sample, all with observed equivalent widths greater than or equal to 0.40 A; three of the Ly-alpha lines have Ly-beta counterparts. The number of Ly-alpha lines observed in the spectrum of PKS 0405-12 is within 1 sigma of the number predicted on the basis of previous HST observations of 3C 273 and of H1821 + 643. Combining the HST observations of 3C 273, H1821 + 643, and PKS 0405-12, we estimate the local number density of Ly-alpha systems with rest equivalent widths larger than 0.32 A to be about 15 +/- 4 Ly-alpha lines per unit redshift. Ground-based images reveal a rich field of galaxies in the direction of PKS 0405-12, including many galaxies with the brightnesses and sizes expected if they belong to a cluster associated with the quasar. The quasar spectrum does not show any evidence for absorption at the redshift of the emission lines, indicating a covering factor of less than unity for the halos of galaxies in the cluster around PKS 0405 - 12.

Bahcall, John N.↗

Coordinated Mineralogical and Isotopic Analysis of a Cosmic Symplectite Identified in a Stardust Terminal Particle

Comet Wild-2 samples returned by the Stardust spacecraft contain a chemically diverse mixture of material, underscoring the complex nature of comets. Studies of entire Stardust aerogel tracks afford the opportunity to examine the fine-grained particle fragments distributed along the length of the track as well as the terminal particles. Previous TEM characterization of a terminal particle (TP) in track #147 revealed a symplectically intergrown iron sulfide and oxide assemblage. Mineralogically similar assemblages, known as cosmic symplectites (COS, formerly termed "new-PCP"), have only been identified in the primitive carbonaceous chondrite Acfer 094. Meteoritic COS have isotopically heavy O compositions (delta (sup 17), O-18 = 180per mille) that point to interactions with early solar system primordial water. In this study we report mineralogical and O isotopic measurements of the Wild-2 COS assemblage. Experimental: Track #147 is a "bulbous"-type track (4600 microns long) containing 7 terminal particles. The TPs were removed from the track, embedded in epoxy, and ultramicrotomed. A JEOL 2500SE 200 keV field-emission scanning-transmission electron microscope was used to obtain quantitative elemental maps and detailed mineralogical characterization. Following TEM analysis, two thin sections of TP4 (12 microns) were analyzed for O isotopes by raster ion imaging with the JSC NanoSIMS 50L. All three O isotopes were measured simultaneously using electron multipliers. San Carlos olivine grains were used as isotopic standards. Results and Discussion: The COS in the Wild-2 track #147 TP4 sample consists of symplectically intergrown pentlandite and nanocrystalline maghemite which coexists with high-Ca pyroxene with Na and Cr (kosmochlor component). This kosmochlor component could have a nebular origin and be precursors to type II chondrules in ordinary chondrites. Yet pentlandite is not a stable phase in the nebula. The COS in Acfer 094 also consists of pentlandite, but contains magnetite [4] rather than the more oxidized maghemite observed in the Wild-2 COS. The Acfer 094 COS display heavy O isotopic compositions that are the result of sulfidization and oxidation of Fe, Ni-metal grains and sulfides by O-17- and O-18-rich water in the solar nebula or possibly on the parent body. The O isotopic composition of the Wild-2 COS, however, is indistinguishable from terrestrial, indicating it was not altered by the same primordial aqueous reservoir as the Acfer 094 COS. The alteration could have occurred on the parent body by isotopically equilibrated ice. The mineralogy and petrography of Wild-2 samples suggests an incomplete or nascent hydration process. In future work we will analyze S isotopes in the track #147 TP4 COS and search for additional COS in Stardust samples.

Nguyen, A. N.↗

Inflight Microbial Monitoring- An Alternative Method to Culture Based Detection Currently Used on the International Space Station

Previous research has shown that potentially destructive microorganisms and human pathogens have been detected on the International Space Station (ISS). The likelihood of introducing new microorganisms occurs with every exchange of crew or addition of equipment or supplies. Microorganisms introduced to the ISS are readily transferred between crew and subsystems (i.e. ECLSS, environmental control and life support systems). Current microbial characterization methods require enrichment of microorganisms and at least a 48-hour incubation time. This increases the microbial load while detecting only a limited number of the total microorganisms. The culture based method detects approximately 1-10% of the total organisms present and provides no identification. To identify and enumerate ISS microbes requires that samples be returned to Earth for complete analysis. Therefore, a more expedient, low-cost, in-flight method of microbial detection, identification, and enumeration is warranted. The RAZOR EX, a ruggedized, commercial off the shelf, real-time PCR field instrument was tested for its ability to detect microorganisms at low concentrations within one hour. Escherichia coli, Salmonella enterica Typhimurium, and Pseudomonas aeruginosa were detected at low levels using real-time DNA amplification. Total heterotrophic counts could also be detected using a 16S gene marker that can identify up to 98% of all bacteria. To reflect viable cells found in the samples, RNA was also detectable using a modified, single-step reverse transcription reaction.

Inflight Microbial↗