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At least 397 records · Page 22

Development of Analysis Methods that Integrate Numeric and Textual Equipment Reliability Data

Within the Light Water Reactor Sustainability (LWRS) program, the Risk-Informed Systems Analysis (RISA) Pathway is performing collaborative research on the development and deployment of technologies designed to assist operating nuclear power plants (NPPs) to reduce operating costs improve plant reliability and availability. One of the RISA research areas is focusing on the development of methods and tools designed to optimize plant operations (e.g., maintenance/replacement schedules, optimal maintenance postures for plant structures, systems, and components [SSCs]) in a manner that is more cost effective than current approaches and makes better use of available SSC health data. The Risk-Informed Asset Management (RIAM) project targets this research area by creating a direct bridge between component equipment reliability (ER) data and system engineer decision making regarding maintenance activity scheduling and component aging management. In this respect, one challenge that NPP system engineers are facing is that the amount of ER data being continuously generated is not only extremely large in size, but it comes in different forms: textual (e.g., condition or maintenance reports) and numeric (e.g., generated by monitoring systems). All these data elements provide them with valuable insights and information regarding: 1) the discovery of anomalous behaviors or degradation trends, 2) the identification of the possible causes behind such behaviors/trends, and 3) the prediction of their direct consequences. However, several challenges have proved to be roadblocks to this process. While some of these challenges are technical in nature (i.e., data are often distributed over several physical servers/databases), others are conceptual in nature: data elements come in different formats (e.g., numeric or textual), and measured values have different scales (e.g., vibration spectra and oil temperature). The activities performed by the RIAM project during FY23 directly tackles the need to simultaneously integrate the analysis of ER data in all its forms, numeric and textual. Note that such task has never been performed before due to the complexity of the systems under consideration but, most importantly, because of the technical challenges behind the harmonization of ER data formats and the lack of adequate computational methods to analyze them. Our approach borrows ideas and concepts from the medical field where integration of several data sources is vital to assist medical practitioners to perform correct diagnosis and indicate optimal treatments. In our view a NPP asset is equivalent to a patient in a medical context. The main difference is the complexity of a human body is a magnitude more complex when compared to typical assets commonly present in NPPs (e.g., centrifugal pumps, or motor operated valves). This simplifies our first requirement when analyzing heterogenous ER data formats: to put data into “context”. Context is here intended as the additional piece of information that is needed by ER data analysis tools to understand what these data elements are referring to, i.e., which king of knowledge they are generating. In our context, this knowledge can be translated into models that capture the form and functional architecture of assets/systems, their dependencies, and how they interact. These models actually emulate the knowledge that that NPP system engineers possess about assets and systems; this is their key of success when analyzing ER data, their challenge is ability to handle large amount of data. Here, we employ model-based system engineering (MBSE) models of systems and assets to represent and capture their architecture and functional, i.e. cause-effect, relations. Then, ER data elements are processed by identifying first of all which elements of the developed MBSE elements they are referring to. For numeric ER data this task is fairly easy since it is possible to precisely pinpoint what MBSE elements the corresponding sensor are observing (e.g., bearing temperature of a centrifugal pump). Task is much harder for textual data since the information contained in issue or maintenance reports needs to “be understood” by a computational tool. Here we called this process as “knowledge extraction”. Once again, we borrow the experience in the medical field where methods to extract knowledge from textual data have been developed in the past decade. The missing element for us is the availability of a complete dictionary of NPP related concepts (in addition to the MBSE models presented earlier) that can put “text into context”. In FY23, such dictionary has been developed along with all the computational elements required for knowledge extraction. Lastly, once numeric and textual ER data elements have been processed and “understood”, then the last step is the discovery of possible cause-effect relations among them. This is performed by observing if a logical connection through the MBSE models exists, and if the

97 MATHEMATICS AND COMPUTING↗

DOE Bioenergy Technologies Office (BETO) 2023 Project Peer Review: WBS 2.4.1.100 Bench Scale Research & Development

Bench Scale Integration develops and optimizes fermentation processes to produce bio-based fuels and chemicals for commercial scale-up. The project uses fermentation science to achieve high titers and production rates by, for example, manipulating how the microorganisms are fed biomass sugars and nutrients, modifying fermentation conditions (pH, temperature, aeration) or developing online control strategies for better fermentation operations and high titer, rates, and yield (TRY). For this period of performance, we continued our development of a commercial-ready 2,3-butanediol (BDO) fermentation from biomass sugars utilizing NREL's proprietary Zymomonas mobilis microorganism. The engineered Z. mobilis can use all the main sugars in corn stover biomass, which are glucose, xylose, and arabinose. BDO is a versatile, low-carbon chemical which can be catalytically upgraded to a variety of hydrocarbon fuels and chemicals. The project had three goals during this review period; evaluate the technical feasibility of using whole slurry pretreated corn stover to achieve the techno-economic analysis (TEA) performance goals, continue optimizing a liquor-based fed-batch fermentation process for high titer, and develop strategies to enable scale-up. After evaluating different iterations of a whole slurry fermentation that did not meet the TEA goals, a Go/No-Go decision was made to pivot to liquor-only with new TEA performance targets, the main one being 140 g/L titer. We successfully met this goal, producing 141 g/L BDO at 1 g/L-hr productivity and 84% process yield. This titer and productivity attracted industrial interest to scale the fermentation resulting in a Technology Commercialization Fund project award in 2022. We used an NREL developed near-infra red (NIR) spectroscopy method for rapid analysis which allowed for changes to aeration levels and sugar feeding during the fermentation to maximize BDO production. The NIR analysis can be done using a hand-held spectrometer, essentially taking the analysis on to the plant floor during scale-up and preliminary work shows the feasibility of using an online probe for continuous monitoring and control. The other scale-up tool is mapping oxygen transfer coefficient (kLa) and oxygen transfer rate (OTR) in various vessels to find conditions that match the optimized 500 mL vessels. Showing a correlation to the mapping work, which is done with water and a dissolved oxygen probe, can reduce the risk of failed fermentations during scale-up. Our end-of-project goal is to meet the design target BDO titer (140-150 g/L) from DMR corn stover liquor at 1000L or larger scale to demonstrate BDO process design case and transfer the technology to industrial fermentation stakeholders for commercialization.

2 3-butanediol↗

Development of the 3 m{sup 3} Low Level Radioactive Waste Container in Taiwan - 20037

Chinshan Nuclear Power Plant stopped its business operation in July, 2019, and Kuosheng Nuclear Power Plant will also be permanently shut down in 2023. Nuclear Reactor Facilities Regulation Act requires a nuclear facility must be dismantled in 25 years after being permanently ceased operation. According to the approved decommissioning plan of Chinshan Nuclear Power Plant and the proposed one of Kuosheng Nuclear Power Plant, the 3 m{sup 3} low level radioactive waste container is planned to store the low level radioactive wastes with higher radiation produced during the decommissioning. However, 3 m{sup 3} low level radioactive waste containers are not used or developed in Taiwan before. To meet such need, Institute of Nuclear Energy Research develops a new type of 3 m{sup 3} low level radioactive waste container and performs the corresponding analyses and tests based on Guidelines of Applying the Usage License of Low Level Radioactive Waste Containers and Regulations for the Safe Transport of Radioactive Material. In this article, it provides the information of the low level radioactive waste containers that have been approved or is under reviewing in Taiwan, and so do the design specifications of the developed 3 m{sup 3} waste container. Besides, the numerical evaluation and test results of the developed 3 m{sup 3} waste container are also included in this article. Based on the evaluation and test results, the developed 3 m{sup 3} waste container satisfies the requirements of the storage container and industrial package type 2. Institute of Nuclear Energy Research will submit the usage license applications to the authority this year, and the usage licenses would be granted in 2021, expectedly. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Issue Resolution During the Development of the Performance Assessment for the Savannah River Site Saltstone Disposal Facility - 20125

In 2019, Savannah River Remediation developed a revision to the performance assessment (PA) on behalf of the U.S. Department of Energy (DOE) Savannah River Operations Office (SR) for the near-surface disposal of low-level waste at the Savannah River Site (SRS) Saltstone Disposal Facility (SDF). Soluble waste from SRS Tank Farms undergoes salt processing to remove cesium and other high-activity constituents. The low-activity decontaminated salt solution (DSS) is then immobilized by mixing it into a cementitious waste form known as saltstone. After mixing, the saltstone is poured into leak-tight concrete vaults, known as saltstone disposal units (SDUs), where the waste form cures. By the time of facility closure, the SDF is expected to consist of 15 SDUs with a combined capacity of 1.06 E+09 L (280 Mgal) of cured saltstone. The facility operates under a Disposal Authorization Statement from DOE and a permit from the South Carolina Department of Health and Environmental Control (SCDHEC). Since the start of operations in 1990, the SDF has received almost 6.7 E+07 L (18 Mgal) of DSS, resulting in the safe disposal of 2.7 E+16 Bq (7.3 E+05 Ci) of activity. Due to the radioactive decay of short-lived contaminants, the total remaining activity in the disposed waste is estimated to be approximately 1.4 E+16 Bq (3.9 E+05 Ci), as of September 2018. The Disposal Authorization Statement requires a demonstration that the system of engineered and natural features of the disposal facility will limit releases from the facility and be protective of human health and the environment for at least the next 1,000 years. The long-term performance of the facility was evaluated under the requirements of the DoE's Radioactive Waste Management Manual (US DOE Manual 435.1-1). Simulations were performed to demonstrate that the disposal facility would meet performance objectives specified in the manual. The evaluation was based on numerical models that simulate the releases of contaminants from the saltstone waste form. Contaminants were transported through groundwater and air pathways to points of assessment to evaluate compliance (i.e., 100 m from the SDUs). In addition, the potential consequences of an inadvertent human intrusion (IHI) were also evaluated. A number of issues were overcome during the development of these simulations. These issues were identified as part of internal technical reviews. Simulations are developed by people and people make mistakes, so the internal technical review process is a vital step in PA development. Specific examples of resolved issues include a unit-conversion error, an inappropriate definition for a model boundary condition, a model time-stepping issue, and an error in the calculation for the buildup of contaminants in soil. Actions taken to address these issues resulted in an improved product with a better supported technical basis and more defensible results. The identification and correction of these issues are discussed. By understanding these issues, model developers and technical reviewers working on PAs in the future may avoid repeating these types of mistakes. Transparency with respect to these mistakes builds trust between waste management sites, regulators, and stakeholders. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Technical Evaluation of the Department of Energy Research and Development Activities in Underground Research Laboratories - 20287

Congress created the U.S. Nuclear Waste Technical Review Board (NWTRB) in the 1987 Nuclear Waste Policy Amendments Act (Public Law 100-203) to evaluate the technical and scientific validity of activities undertaken by the Secretary of Energy to implement the Nuclear Waste Policy Act. Since 2012, DOE has collaborated in research conducted in several underground research laboratories (URLs) located in Europe and Asia. According to DOE, these international collaborations have been beneficial to its spent nuclear fuel (SNF) and high-level waste radioactive (HLW) disposal research program, particularly after the termination of the Yucca Mountain repository program when DOE began generic research on alternative host rocks (crystalline, clay, and salt) and repository environments very different from those at Yucca Mountain. In accordance with its mandate, the NWTRB is reviewing the DOE research and development (R and D) activities related to URLs. The NWTRB's review is documented in a report to Congress and the Secretary of Energy that will be released in January 2020, and this paper summarizes the NWTRB review and findings. The NWTRB held a fact-finding meeting with DOE and subsequently held a workshop on international URL collaborations in April of 2019. Based on the presentations and discussions at the workshop and at the fact-finding meeting, as well as information from reports published by DOE and others, there are four principal findings related to DoE's URL-related R and D activities. First, DOE participation in URL-related international research greatly benefits the U.S. geologic disposal R and D program by furthering its understanding of generic and site-specific disposal issues relevant to alternative repository host rocks and environments. DOE-funded R and D activities also are benefiting the URL-related research of other countries, especially in the area of complex analytical and numerical model/software development. Second, the more developed repository programs in other countries have focused on creating and strengthening their safety cases and making them transparent to the public. Repository programs in other countries use URLs to explain the technical bases underlying their safety cases, periodically reassess knowledge gaps and define new activities to strengthen the technical bases, and demonstrate the technology that will allow implementation of the proposed safety concept. Third, countries with more developed geologic disposal programs have found domestic URLs essential to their repository programs. DOE needs domestic URLs to advance geologic disposal efforts over the next decades and further its ability to train the next generation of scientists, engineers, and skilled technical workers. Fourth, DoE's international URL collaborations have advanced its generic disposal R and D program, including development of modeling capabilities recognized internationally as state-of-the-art, but further work on its coupled thermal-hydrological-mechanical-chemical models and URL- and laboratory-based research can strengthen its program. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Overview of Salt Repository Research and Development for Spent Nuclear Fuel and High-level Nuclear Waste in the United States - 20307

This paper summarizes the current US Department of Energy Office of Nuclear Energy's (DOE-NE) work towards developing and executing a research and development program that addresses both scientific and technical issues related to long-term disposal of spent nuclear fuel (SNF) and high-level waste (HLW) in a hypothetical bedded salt based geological repository. A primary goal of the program is to create a generic Geologic Disposal Safety Assessment (GDSA) Framework that can be used to help guide decisions on siting a possible future bedded salt repository. The generic GDSA work includes analysis of the impacts of heat generation caused by decay of short-lived radionuclides. We report progress in four primary areas. First, we discuss the development and recent modifications of a research and development road-map. Second, we briefly describe an experimental approach to better understand thermal processes in salt. Third, we highlight collaborations with the international research community that leverage salt-based repository science around the world. Finally, we discuss how our findings are being used to aid in the development of a generic safety assessment for a bedded salt repository containing SNF and HLW. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Development of a second COMB dipole magnet with STAR wires

Rare-Earth Barium Copper Oxide (REBCO) coated conductors are an attractive option for application in high-field accelerator magnets due to their high critical field and the convenience of fabrication without heat treatment compared to some other superconductors. Fermilab plays a vital role in superconducting accelerator magnet R&D under the framework of the U.S. Magnet Development Program. An integral part of that program is the accelerator magnet development based on REBCO conductors to demonstrate self-fields of 5 T or greater compatible with operation in hybrid configurations to generate fields beyond 16 T for future High Energy Physics (HEP) applications. A small, two-layer REBCO dipole magnet based on the Conductor on Molded Barrel (COMB) magnet technology, developed at Fermilab with Symmetric Tape Round (STAR®) wires from AMPeers LLC was recently fabricated and tested in liquid helium, demonstrating the design feasibility. A second dipole magnet based on the same technology is currently under development at Fermilab. The coil has a 60-mm clear bore and a 120-mm OD and consists of six layers of STAR® conductor, which allows to significantly increase the magnetic field compared to the previous design, and potentially probe the 5 T self-field target. This presentation reports the progress in the magnet development.

Kashikhin, Vadim [Fermilab]↗

Hanford Tank Waste Technology Development Integration and Innovation Synergy

The Chief Technology Office (CTO) within Hanford Tank Waste Operations & Closure (H2C) identifies technology development needs and develops solutions for tank waste treatment. One key element of the technology development (TD) program is the annual Technology Development Integrated Coordination (TDIC) meeting series. The main goal of these meetings is to inform the National Laboratories and other entities of the needs identified by the end users of the technology. In turn, the meetings provide an opportunity for National Laboratories and others to provide insight into potential solutions. This dynamic facilitates dialogues that frequently identify not only new, innovative approaches, but also enhancements to ongoing technology development projects.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DEVELOPMENT AND DEMONSTRATION TESTBED FOR THE REMOTE OPERATIONS AND MONITORING OF MICROREACTORS

The nuclear industry is rapidly developing many advanced-reactor concepts for near-term deployment in both traditional and non-traditional nuclear-powered applications. One such category of advanced reactor is the microreactor, a class of reactor with less than 20MWth power output, intended for applications where the economics or logistics of traditional power sources are difficult. This includes applications such as remote communities, mining sites, defense installations, or humanitarian and disaster-relief missions. One key enabling feature for the successful deployment of microreactors is a remote operations capability. Remote operations provide monitoring and control capabilities which can significantly reduce staffing costs by eliminating the need for licensed operators at each reactor facility and improve the economic viability for microreactor deployment. A remote concept of operations is not currently an established capability in the nuclear industry. In addition, no demonstration microreactor is expected to complete construction or go critical until at least 2026. This leaves two major capability gaps: the successful demonstration of a remote concept of operations for microreactors and a test bed suitable for said demonstration. Both gaps must be addressed in order to advance the remote concepts of nuclear operation and, more broadly, microreactors themselves from paper to reality. This paper aims to fill these gaps and describes a test bed that would support development and deployment of a remote concept of nuclear operations, initial experimental results from that test bed, and the application of the test bed and experimental results for a digital-twin-based remote concept of operations underdevelopment at Idaho National Laboratory (INL). The platform chosen as a remote concept of nuclear operations test bed is the Single Primary Heat Extraction and Removal Emulator, known as SPHERE, located at INL. SPHERE is a small-scale non-nuclear test bed that emulates thermal behavior of a microreactor. The small-scale and non-nuclear nature of SHPERE limit safety concerns associated with remote operations while still providing the physical response representative of a microreactor. A network connection was added to SPHERE that enables remote-monitoring capability. This allows for real-time data streaming to networked workstations, data historians, and human-machine interfaces (HMIs). These are all critical components in a remote concept of operations, thus providing a robust development and demonstration platform. An initial experiment was performed using the SPHERE remote operations testbed. This included running a comprehensively instrumented SPHERE through a series of steady-state and transient operating scenarios in both normal and abnormal operating conditions, all while streaming live test data to a remote HMI and data warehouse. This initial experiment served three purposes: (1) characterizing the response of SPHERE, (2) demonstrating the remote connection to SPHERE, and (3) providing a baseline data set for development of a digital-twin-based remote concept of operations that is under development at INL.

22 - GENERAL STUDIES OF NUCLEAR REACTORS↗

DEVELOPMENT OF AN INTEGRATED SECURITY AND SAFETY MONITORING SYSTEM FOR SPENT NUCLEAR FUEL AND HIGH-LEVEL WASTE TRANSPORTATION

This paper provides an overview of the progress to date, and discussion of the path forward, related to designing, fabricating, and testing an integrated security and safety monitoring system (ISSMS) for railcars used to transport spent nuclear fuel (SNF) and high-level radioactive waste (HLW) in the United States. The system will comply with the US Department of Energy’s (DOE) Order 460.2B “Departmental Materials Transportation Management” and the Association of American Railroads’ (AAR) standard S-2043 “Performance Specification for Trains Used to Carry High-level Radioactive Material” [1] developed specifically for railcars used to transport high-level radioactive material (HLRM). DOE is in the process of developing and testing an ISSMS that will satisfy both DOE requirements and AAR standards. DOE has already developed railcar designs for transportation of HLRM. In 2024, DOE’s Atlas railcar project completed the design, fabrication and testing of three railcar types: transportation cask-carrying, buffer, and security escort, resulting in AAR conditional approval to operate on freight rail networks in North America. DOE decided to combine the required security and safety systems into one system, and this combined system is the subject of the current effort. DOE is developing and proposes to implement the ISSMS for these railcars as part of the build out of the railcar fleet. DOE began planning for development of the ISSMS in February 2020. The project is divided into seven phases starting with conceptual design and continuing through production design, as shown in Figure 1. An earlier version of the system was tested as part of the Atlas railcar consist demonstration test run in 2023. This paper describes the design features and system testing using the Atlas project railcars, and laboratory testing completed to date. The paper will also describe the activities planned to support the DOE project to ship the High Burn-Up Research Cask (HBRC) in 2027.

Schultze, Michael [ORNL] (ORCID:0000000283205671)↗

Autonomous experimentation systems for materials development: A community perspective

Solutions to many of the world's problems depend upon materials research and development. However, advanced materials can take decades to discover and decades more to fully deploy. Humans and robots have begun to partner to advance science and technology orders of magnitude faster than humans do today through the development and exploitation of closed-loop, autonomous experimentation systems. This review discusses the specific challenges and opportunities related to materials discovery and development that will emerge from this new paradigm. Our perspective incorporates input from stakeholders in academia, industry, government laboratories, and funding agencies. We outline the current status, barriers, and needed investments, culminating with a vision for the path forward. We intend the article to spark interest in this emerging research area and to motivate potential practitioners by illustrating early successes. We also aspire to encourage a creative reimagining of the next generation of materials science infrastructure. To this end, we frame future investments in materials science and technology, hardware and software infrastructure, artificial intelligence and autonomy methods, and critical workforce development for autonomous research.

36 MATERIALS SCIENCE↗

Small Signaling Peptides in Sorghum bicolor : Integrating Phylogeny and Gene Expression to Characterize Roles in Stem Development

Small signaling peptides (SSPs) are important regulators of plant growth, development, and responses to biotic and abiotic stress, yet their role in the C4 grass Sorghum bicolor is largely uncharacterized. To help fill this knowledge gap, 219 sorghum genes that encode SSPs were identified based on SSP sequences previously identified in Arabidopsis thaliana, Zea mays, Oryza sativa, Triticum aestivum , and Brachypodium distachyon . The 219 sorghum SSP-encoding genes were assigned to 19 gene families, analyzed for the presence of motifs, and aligned with genes that encode SSPs in other plants using phylogenetic analysis. Sorghum genes in 12 of the 19 SSP gene families had not been previously characterized. Expression of the 219 SSP-encoding genes in sorghum organs, during stem development, and in stem tissues and cell types revealed distinct spatial, temporal, and developmental patterns of expression. Genes associated with the SbCEP and SbRGF families were preferentially expressed in roots, whereas SbEPF genes were expressed in stem epidermal and pith parenchyma cells and panicles. The expression of genes during bioenergy sorghum stem growth and development was investigated because stems account for ~80% of harvested biomass and serve as conduits for water and nutrient transport between leaves and roots. During stem development, 28 SSP genes in several families ( CLE, EPF, CEP, GASS, PSY, ES, PSK, CAPE, POE ) were expressed at higher levels in zones of cell proliferation. For example, the TDIF homologs SbCLE41 and SbCLE42 were expressed at high levels in nascent stem nodes where they may regulate vascular bundle cambial activity and cell differentiation. A different set of 15 genes in the CIF, POE, CAPE, PSY, CEP, RALF , and CLE families were expressed at higher levels in zones of stem tissue differentiation highlighted by elevated expression of five SbRALFR s in the stem nodal plexus. Cell type–specific expression of many sorghum genes that encode SSPs was observed in fully elongated internodes indicating gene expression is regulated with high spatial resolution. Overall, the results provide a foundation of information for analysis of SSP function in sorghum that can be integrated with knowledge of sorghum gene regulatory networks to modulate traits important for production of sorghum crops.

bioenergy sorghum↗

Air quality impacts from the development of unconventional oil and gas well pads: Air toxics and other volatile organic compounds

Unconventional oil and natural gas development (UOGD) has expanded rapidly across the United States in recent decades and raised concerns about associated air quality impacts. While significant effort has been made to quantify methane emissions, relatively few observations have been made of Volatile Organic Compounds (VOCs), especially during drilling and completion of new wells. Extensive air monitoring during development of several large, multi-well pads in Broomfield, Colorado, in the Denver-Julesburg Basin, provides a novel opportunity to examine changes in local air toxics and other VOC concentrations during well drilling and completions and production. These operations offer an especially useful case to study as several management practices were implemented to reduce emissions (e.g., electrified, grid-powered drill rigs and closed loop fluid handling systems to reduce truck traffic and limit fluid handling on the pad). With simultaneous measurements of methane and 50 VOCs from October 2018 to December 2022 at as many as 19 sites near well pads, in adjacent neighborhoods, and at a more distant reference location, we identify impacts from each phase of well development and production. Use of weekly, time-integrated canisters, a Proton Transfer Reaction Mass Spectrometer (PTR-MS), continuous photoionization detectors (PID) to trigger canister collection upon detection of VOC-rich plumes, and an instrumented vehicle, provided a powerful suite of measurements to characterize both transient plumes and longer-term changes in air quality. Prior to the start of well development, VOC gradients were small across Broomfield. Once drilling commenced, concentrations of oil and gas (O&G) related VOCs, including alkanes and aromatics, increased around active well pads. Concentration increases were clearly apparent during certain operations, including drilling, coil tubing/millout operations, and production tubing installation. Emissions of C 8 –C 10 n-alkanes during drilling operations highlighted the importance of VOC emissions from synthetic drilling mud chosen to reduce odor impacts. More than 90 samples were collected of transient plumes. Using composition measurements, meteorological data, and information about well pad activities, these plumes were connected with specific UOGD operations including drilling, flowback, and production equipment maintenance. The chemical signatures of these plumes differed by operation type (e.g., C 8 –C 10 n-alkanes constituted a larger fraction of measured VOCs in drilling-related plumes). Concentrations of individual, oil and gas-related VOCs in these plumes were often several orders of magnitude higher than in background air, with maximum ethane and benzene concentrations of 79,600 and 819 ppbv, respectively. Because these plumes typically impact a monitoring site for just several minutes, they are easily missed by slower-responding instruments. Study measurements highlight future emission mitigation opportunities during UOGD operations, including better control of emissions from shakers that separate drill cuttings from drilling mud, production separator maintenance operations, and periodic emptying of sand cans during flowback operations.

54 ENVIRONMENTAL SCIENCES↗

Differential Etv2 threshold requirement for endothelial and erythropoietic development

Endothelial and erythropoietic lineages arise from a common developmental progenitor. Etv2 is a master transcriptional regulator required for the development of both lineages. However, the mechanisms through which Etv2 initiates the gene-regulatory networks (GRNs) for endothelial and erythropoietic specification and how the two GRNs diverge downstream of Etv2 remain incompletely understood. Here, by analyzing a hypomorphic Etv2 mutant, we demonstrate different threshold requirements for initiation of the downstream GRNs for endothelial and erythropoietic development. We show that Etv2 functions directly in a coherent feedforward transcriptional network for vascular endothelial development, and a low level of Etv2 expression is sufficient to induce and sustain the endothelial GRN. In contrast, Etv2 induces the erythropoietic GRN indirectly via activation of Tal1, which requires a significantly higher threshold of Etv2 to initiate and sustain erythropoietic development. These results provide important mechanistic insight into the divergence of the endothelial and erythropoietic lineages.

59 BASIC BIOLOGICAL SCIENCES↗

AI-based language models powering drug discovery and development

The discovery and development of new medicines is expensive, time-consuming, and often inefficient, with many failures along the way. Powered by artificial intelligence (AI), language models (LMs) have changed the landscape of natural language processing (NLP), offering possibilities to transform treatment development more effectively. Here, we summarize advances in AI-powered LMs and their potential to aid drug discovery and development. We highlight opportunities for AI-powered LMs in target identification, clinical design, regulatory decision-making, and pharmacovigilance. We specifically emphasize the potential role of AI-powered LMs for developing new treatments for Coronavirus 2019 (COVID-19) strategies, including drug repurposing, which can be extrapolated to other infectious diseases that have the potential to cause pandemics. Finally, we set out the remaining challenges and propose possible solutions for improvement.

60 APPLIED LIFE SCIENCES↗

Cooperation or Rivalry? Impact of alternative development pathways on India's long-term electricity generation and associated water demands

The world has witnessed a significant shift in the last decade in the way countries interact with each other. A cooperation based international paradigm is being challenged by countries engaging in rivalry with each other in the name of their national interests. What developmental pathway the world charts in the future is going to be path defining for global energy and emissions use. India, expected to play a critical role in the emissions mitigation debate, would contribute to and would be impacted by these pathways. In India specific literature, however, little attention has been paid to development pathways related uncertainties and their impact on energy futures. We seek to address this gap by modelling India's electricity futures and associated water demands under different development pathways. Our analysis shows that alternative development pathways would have significantly different implications for the energy access, electricity generation mix, associated water withdrawals, and carbon dioxide emissions in India. We conclude by arguing that energy, emissions and water policies should be conceived with an explicit understanding of uncertainties related to potential development pathways that the world and India can chart.

India, Electricity-Water Nexus, Development Pathwa↗

Go slow to go fast? A review of the impacts of permitting on large-scale solar project development

State and local permitting challenges could impede the ability of large-scale solar (LSS) to meet growing electricity demand in the United States. Here, we review research that explores LSS permitting and its impacts on the pace and scale of LSS project development. Research on LSS permitting is relatively scarce, such that we support our review with research in the context of wind permitting, where appropriate. Further, few studies attempt to rigorously quantify the effects of permitting on the pace and scale of LSS project development. The available evidence allows us to identify various hypotheses and identify gaps for further research. Our review suggests that differences in permitting policies, regulations, and ordinances explain relatively little variation in LSS permitting and development outcomes across jurisdictions, except where jurisdictions implement rules designed to impede LSS. The evidence suggests LSS permitting challenges largely accrue during the implementation of permitting processes. Recent research suggests that community opposition to project development is a key driver of LSS permitting challenges, given that project opponents often use permitting processes to translate opposition into legal action. We call on future researchers to more concretely describe the LSS permitting challenge and to identify the specific actors responsible for implementing solutions.

Community opposition↗

The synergy between stakeholders for cellulosic biofuel development: Perspectives, opportunities, and barriers

While understanding individual stakeholders' perspectives on the adoption and conversion to a biofuel-based landscape has been a subject of many previous studies on biofuels, there has been relatively little attention given to understanding how the interaction between multiple stakeholders involved in biofuel development could influence the widespread adoption of biofuel production. Here, this paper analyzes the key stakeholder interactions utilizing various data sources including survey results, social media posts, and empirical and theoretical analyses. An intensive review is conducted for a number of surveys and research papers on different aspects of biofuel development such as land use choices, biorefinery and transportation, infrastructure development, consumer priorities, environmental impacts, etc. Following that, a stakeholder synergy approach is applied to synthesizing typical responses of stakeholders, such as producers, consumers, biorefineries, rural communities, and the government, and discussing how their responses influence each other's decisions and the overall system performance. Based on the findings of inadequate stakeholder synergy, it is recommended that new surveys and further research should be conducted to understand why synergy between stakeholders in biofuel development is absent. Additionally, this paper provides research perspectives, including (1) applying cutting-edge text-mining techniques to conduct sentiment analysis, and research and public attention analysis; (2) using an agent-based model to simulate stakeholder interactions and understand the factors that influence stakeholder synergy and the emergence of a bioeconomy.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗