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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 397 records · Page 22
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Is the Kok effect a respiratory phenomenon? Metabolic insight using 13 C labeling in Helianthus annuus leaves

The Kok effect is a well-known phenomenon in which the quantum yield of photosynthesis changes abruptly at low light. This effect has often been interpreted as a shift in leaf respiratory metabolism and thus used widely to measure day respiration. However, there is still no formal evidence that the Kok effect has a respiratory origin. Here, both gas exchange and isotopic labeling were carried out on sunflower leaves, using glucose that was 13 C-enriched at specific C-atom positions. Position-specific decarboxylation measurements and NMR analysis of metabolites were used to trace the fate of C-atoms in metabolism. Decarboxylation rates were significant at low light (including above the Kok break point) and increased with decreasing irradiance below 100 µmol photons m –2 s –1 . The variation in several metabolite pools such as malate, fumarate or citrate, and flux calculations suggest the involvement of several decarboxylating pathways in the Kok effect, including the malic enzyme. Our results show that day respiratory CO 2 evolution plays an important role in the Kok effect. Furthermore, the increase in the apparent quantum yield of photosynthesis below the Kok break point is also probably related to malate metabolism, which participates in maintaining photosynthetic linear electron flow.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2016

Multi-level regulation of the chloroplast ATP synthase: the chloroplast NADPH thioredoxin reductase C (NTRC) is required for redox modulation specifically under low irradiance

The chloroplast ATP synthase is known to be regulated by redox modulation of a disulfide bridge on thec-subunit through the ferredoxin–thioredoxin regulatory system. We show that a second enzyme, therecently identified chloroplast NADPH thioredoxin reductase C (NTRC), plays a role specifically at low irradi-ance. Arabidopsis mutants lacking NTRC (ntrc) displayed a striking photosynthetic phenotype in which feed-back regulation of the light reactions was strongly activated at low light, but returned to wild-type levels asirradiance was increased. This effect was caused by an altered redox state of the c-subunit under low, butnot high, light. The low light-specific decrease in ATP synthase activity in ntrc resulted in a buildup of thethylakoid proton motive force with subsequent activation of non-photochemical quenching and downregu-lation of linear electron flow. We conclude that NTRC provides redox modulation at low light using the rela-tively oxidizing substrate NADPH, whereas the canonical ferredoxin–thioredoxin system can take over athigher light, when reduced ferredoxin can accumulate. Based on these results, we reassess previous modelsfor ATP synthase regulation and propose that NTRC is most likely regulated by light. We also find that ntrcis highly sensitive to rapidly changing light intensities that probably do not involve the chloroplast ATP syn-thase, implicating this system in multiple photosynthetic processes, particularly under fluctuating environ-mental conditions.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Linking the Dynamic Response of the Carbon Dioxide-Concentrating Mechanism to Carbon Assimilation Behavior in Fremyella diplosiphon

ABSTRACT Cyanobacteria use a carbon dioxide (CO 2 )-concentrating mechanism (CCM) that enhances their carbon fixation efficiency and is regulated by many environmental factors that impact photosynthesis, including carbon availability, light levels, and nutrient access. Efforts to connect the regulation of the CCM by these factors to functional effects on carbon assimilation rates have been complicated by the aqueous nature of cyanobacteria. Here, we describe the use of cyanobacteria in a semiwet state on glass fiber filtration discs—cyanobacterial discs—to establish dynamic carbon assimilation behavior using gas exchange analysis. In combination with quantitative PCR (qPCR) and transmission electron microscopy (TEM) analyses, we linked the regulation of CCM components to corresponding carbon assimilation behavior in the freshwater, filamentous cyanobacterium Fremyella diplosiphon . Inorganic carbon (C i ) levels, light quantity, and light quality have all been shown to influence carbon assimilation behavior in F. diplosiphon . Our results suggest a biphasic model of cyanobacterial carbon fixation. While behavior at low levels of CO 2 is driven mainly by the C i uptake ability of the cyanobacterium, at higher CO 2 levels, carbon assimilation behavior is multifaceted and depends on C i availability, carboxysome morphology, linear electron flow, and cell shape. Carbon response curves (CRCs) generated via gas exchange analysis enable rapid examination of CO 2 assimilation behavior in cyanobacteria and can be used for cells grown under distinct conditions to provide insight into how CO 2 assimilation correlates with the regulation of critical cellular functions, such as the environmental control of the CCM and downstream photosynthetic capacity. IMPORTANCE Environmental regulation of photosynthesis in cyanobacteria enhances organismal fitness, light capture, and associated carbon fixation under dynamic conditions. Concentration of carbon dioxide (CO 2 ) near the carbon-fixing enzyme RubisCO occurs via the CO 2 -concentrating mechanism (CCM). The CCM is also tuned in response to carbon availability, light quality or levels, or nutrient access—cues that also impact photosynthesis. We adapted dynamic gas exchange methods generally used with plants to investigate environmental regulation of the CCM and carbon fixation capacity using glass fiber-filtered cells of the cyanobacterium Fremyella diplosiphon . We describe a breakthrough in measuring real-time carbon uptake and associated assimilation capacity for cells grown in distinct conditions (i.e., light quality, light quantity, or carbon status). These measurements demonstrate that the CCM modulates carbon uptake and assimilation under low-C i conditions and that light-dependent regulation of pigmentation, cell shape, and downstream stages of carbon fixation are critical for tuning carbon uptake and assimilation.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2019

Ocean Energy Systems Wave Energy Modelling Task: Modelling, Verification and Validation of Wave Energy Converters

The International Energy Agency Technology Collaboration Programme for Ocean Energy Systems (OES) initiated the OES Wave Energy Conversion Modelling Task, which focused on the verification and validation of numerical models for simulating wave energy converters (WECs). The long-term goal is to assess the accuracy of and establish confidence in the use of numerical models used in design as well as power performance assessment of WECs. To establish this confidence, the authors used different existing computational modelling tools to simulate given tasks to identify uncertainties related to simulation methodologies: (i) linear potential flow methods; (ii) weakly nonlinear Froude–Krylov methods; and (iii) fully nonlinear methods (fully nonlinear potential flow and Navier–Stokes models). This article summarizes the code-to-code task and code-to-experiment task that have been performed so far in this project, with a focus on investigating the impact of different levels of nonlinearities in the numerical models. Two different WECs were studied and simulated. The first was a heaving semi-submerged sphere, where free-decay tests and both regular and irregular wave cases were investigated in a code-to-code comparison. The second case was a heaving float corresponding to a physical model tested in a wave tank. We considered radiation, diffraction, and regular wave cases and compared quantities, such as the WEC motion, power output and hydrodynamic loading.

16 TIDAL AND WAVE POWER↗
NASA NTRS1973

Large amplitude flutter of a low aspect ratio panel at low supersonic speeds comparison of theory and experiment

Flutter boundaries, as well as flutter limit cycle amplitudes, frequencies and stresses were computed for a panel of length-width ratio 4.48 exposed to applied in-plane and transverse loads. The Mach number range was 1.1 to 1.4. The method used involved direct numerical integration of modal equations of motion derived from the nonlinear plate equations of von Karman, coupled with linearized potential flow aerodynamic theory. The flutter boundaries agreed reasonably well with experiment, except when the in-plane loading approached the buckling load. Structural damping had to be introduced, to produce frequencies comparable to the experimental values. Attempts to compute panel deflections or stress at a given point met with limited success. There is some evidence, however, that deflection and stress maxima can be estimated with somewhat greater accuracy.

Ventres, C. S.↗
NASA NTRS1974

Aerodynamic influence coefficient method using singularity splines

A numerical lifting surface formulation, including computed results for planar wing cases is presented. This formulation, referred to as the vortex spline scheme, combines the adaptability to complex shapes offered by paneling schemes with the smoothness and accuracy of loading function methods. The formulation employes a continuous distribution of singularity strength over a set of panels on a paneled wing. The basic distributions are independent, and each satisfied all the continuity conditions required of the final solution. These distributions are overlapped both spanwise and chordwise. Boundary conditions are satisfied in a least square error sense over the surface using a finite summing technique to approximate the integral. The current formulation uses the elementary horseshoe vortex as the basic singularity and is therefore restricted to linearized potential flow. As part of the study, a non planar development was considered, but the numerical evaluation of the lifting surface concept was restricted to planar configurations. Also, a second order sideslip analysis based on an asymptotic expansion was investigated using the singularity spline formulation.

Mercer, J. E.↗
NASA NTRS1975

Cold-air experimental investigation of a turbine with blade trailing edge coolant ejection. 1: Single-stage turbine

Tests were made on a 0.762-meter-tip-diameter research turbine to determine the effect of blade coolant flow on its aerodynamic performance. Both stator and rotor blades had trailing-edge slots for coolant ejection. The turbine was tested over a range of speed and pressure ratio. High primary efficiencies, calculated on the basis of primary air only, were obtained. The efficiency attained was identical to that reported for the turbine from a previous investigation were only slotted stator blades where incorporated in the turbine and tested. And it also compares with results for the turbine with solid blading. Independently varying the rotor coolant flow showed that rotor cooling imposed a severe penalty on turbine efficiency. The thermodynamic efficiency, which accounts for the ideal energies of both blade coolant flows, decreased linearly with rotor coolant at a rate of about 0.7 percent per percent rotor coolant fraction.

Schum, H. J.↗
NASA NTRS1976

Some current research in unsteady aerodynamics: A report from the Fluid Dynamics Panel

The highlights of a recent discussion by representatives of the fluid dynamics and structures and materials panels are reported with emphasis on the fundamental aspects of unsteady fluid mechanics. Topics include linearized potential flow theory, transonic flow calculations, unsteady boundary layers, dynamic stall, transonic buffet, and techniques for measuring unsteady pressures.

Mccroskey, W. J.↗
NASA NTRS1976

Impact ejecta on the moon

The partitioning of energy and the distribution of the resultant ejecta on the moon is numerically modeled using a Eulerian finite difference grid. The impact of an iron meteoroid at 15 km/sec on a gabbroic anorthosite lunar crust is examined. The high speed impact induced flow is described over the entire hydrodynamic regime from a time where the peak pressures are 6 Mbar until the stresses everywhere in the flow are linearly elastic, and less than 5 kbar. Shock-induced polymorphic phase changes, (plagioclase and pyroxene to hollandite and perovskite), and the subsequent reversion to low pressure phases are demonstrated to enhance shock wave attenuation. A rate-dependent equation of state is used for describing the hysteretic effect of the phase change. Ballistic equations for a spherical planet are then applied to material with net velocity away from the moon.

Okeefe, J. D.↗
NASA NTRS1980

Free-stream disturbance, continuous Eigenfunctions, boundary-layer instability and transition

A rational foundation is presented for the application of the linear shear flows to transition prediction, and an explicit method is given for carrying out the necessary calculations. The expansions used are shown to be complete. Sample calculations show that a typical boundary layer is very sensitive to vorticity disturbances in the inner boundary layer, near the critical layer. Vorticity disturbances three or four boundary layer thicknesses above the boundary are nearly uncoupled from the boundary layer in that the amplitudes of the discrete Tollmien-Schlicting waves are an extremely small fraction of the amplitude of the disturbance.

Grosch, C. E.↗
NASA NTRS1981

Non-linear propagation in near sonic flows

A nonlinear analysis is developed for sound propagation in a variable-area duct in which the mean flow approaches choking conditions. A quasi-one-dimensional model is used and the nonlinear analysis represents the acoustic disturbance as a sum of interacting harmonics. The numerical procedure is stable for cases of strong interaction and is able to integrate through the throat region without any numerical instability.

Nayfeh, A. H.↗
NASA NTRS1981

Theoretical and experimental supersonic lateral-directional stability characteristics

A program has been initiated at NASA Langley Research Center to assess several methods for estimation of lateral-directional stability. As a basis for comparison, experimental data are presented for a simple wing-body vertical tail configuration. The methods for estimating the characteristics include a second-order shock expansion and panel method (MISLIFT), a slender body and 'first-order' panel method (APAS), and a 'higher-order' panel method for linearized supersonic flow (PAN AIR). The results show that PAN AIR provides accurate estimates of these characteristics at moderate angles of attack for complete configurations with either single or twin vertical tails. APAS provides estimates for complete configurations at zero angle of attack. However, MISLIFT only provides estimates for the simplest body-vertical tail configurations at zero angle of attack.

Lamb, M.↗
NASA NTRS1983

Aeroelastic loads prediction for an arrow wing. Task 2: Evaluation of semi-empirical methods

The development and evaluation of a semi empirical method to predict pressure distributions on a deformed wing by using an experimental data base in addition to a linear potential flow solution is described. The experimental data accounts for the effects of aeroelasticity by relating the pressures to a parameter which is influenced by the deflected shape. Several parameters were examined before the net leading edge suction coefficient was selected as the best.

Wery, A. C.↗
NASA NTRS1983

Soil temperature investigations using satellite acquired thermal-infrared data in semi-arid regions

Thermal-infrared data from the Heat Capacity Mapping Mission satellite were used to map the spatial distribution of diurnal surface temperatures and to estimate mean annual soil temperatures (MAST) and annual surface temperature amplitudes (AMP) in semi-arid east central Utah. Diurnal data with minimal snow and cloud cover were selected for five dates throughout a yearly period and geometrically co-registered. Rubber-sheet stretching was aided by the WARP program which allowed preview of image transformations. Daytime maximum and nighttime minimum temperatures were averaged to generation average daily temperature (ADT) data set for each of the five dates. Five ADT values for each pixel were used to fit a sine curve describing the theoretical annual surface temperature response as defined by a solution of a one-dimensinal heat flow equation. Linearization of the equation produced estimates of MAST and AMP plus associated confidence statistics. MAST values were grouped into classes and displayed on a color video screen. Diurnal surface temperatures and MAST were primarily correlated with elevation.

Day, R. L.↗
NASA NTRS1983

Micromechanically Based Constitutive Relations for Polycrystalline Solids

A basic method to estimate the overall mechanical response of solids which contain periodically distributed defects is presented. The method estimates the shape and growth pattern of voids periodically distributed over the grain boundaries in a viscous matrix. The relaxed moduli are obtained for a polycrytalline solid that undergoes relaxation by grain boundary sliding which accounts for the interaction effects. The overall inelastic nonlinear response at elevated temperatures in terms of a model which considers nonlinear power law creep within the grains, and linear viscous flow in the grain boundaries is discussed.

Nemat-Nasser, S.↗
NASA NTRS1983

The impact of CFD on development test facilities - A National Research Council projection

The results of a National Research Council study on the effect that advances in computational fluid dynamics (CFD) will have on conventional aeronautical ground testing are reported. Current CFD capabilities include the depiction of linearized inviscid flows and a boundary layer, initial use of Euler coordinates using supercomputers to automatically generate a grid, research and development on Reynolds-averaged Navier-Stokes (N-S) equations, and preliminary research on solutions to the full N-S equations. Improvements in the range of CFD usage is dependent on the development of more powerful supercomputers, exceeding even the projected abilities of the NASA Numerical Aerodynamic Simulator (1 BFLOP/sec). Full representation of the Re-averaged N-S equations will require over one million grid points, a computing level predicted to be available in 15 yr. Present capabilities allow identification of data anomalies, confirmation of data accuracy, and adequateness of model design in wind tunnel trials. Account can be taken of the wall effects and the Re in any flight regime during simulation. CFD can actually be more accurate than instrumented tests, since all points in a flow can be modeled with CFD, while they cannot all be monitored with instrumentation in a wind tunnel.

Korkegi, R. H.↗