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At least 397 records · Page 22

Sequence characterization of 5S ribosomal RNA from eight gram positive procaryotes

Complete nucleotide sequences are presented for 5S rRNA from Bacillus subtilis, B. firmus, B. pasteurii, B. brevis, Lactobacillus brevis, and Streptococcus faecalis, and 5S rRNA oligonucleotide catalogs and partial sequence data are given for B. cereus and Sporosarcina ureae. These data demonstrate a striking consistency of 5S rRNA primary and secondary structure within a given bacterial grouping. An exception is B. brevis, in which the 5S rRNA sequence varies significantly from that of other bacilli in the tuned helix and the procaryotic loop. The localization of these variations suggests that B. brevis occupies an ecological niche that selects such changes. It is noted that this organism produces antibiotics which affect ribosome function.

Woese, C. R.↗

Waves observed upstream of interplanetary shocks

The properties of the waves that are present upstream of interplanetary, collisionless, quasi-parallel shocks are described. Two types of such waves have been detected, a higher frequency whistler mode wave and a lower frequency fast mode MHD wave. Both are typically circular or elliptically polarized right-hand waves which propagate along the ambient magnetic field with a 15 deg angle cone. The high frequency waves have sufficient group velocities to outrun the shock, and may be generated by cyclotron resonance with 100 eV to 1 keV shock electrons. The lower frequency waves must be generated locally by particles upstream of the shock, probably by 1-10 keV ions flowing away from the shock. Distinct changes in the spectra of upstream waves as a function of distance from the shock have been noted.

Tsurutani, B. T.↗

The Gaseous Content of the Universe at Zeta less than 1.6

Together with graduate student Hsiao-Wen Chen, I have measured and analyzed structural and morphological parameters of 38 galaxies in eight fields for which sensitive measurements of corresponding Ly(alpha) absorption toward background QSOs are available. These measurements are based on Wide Field Planetary Camera 2 (WFPC2) observations obtained with the Hubble Space Telescope (HST) and provide a first look at how the incidence and extent of tenuous gas around galaxies depends on galaxy luminosity, size, and morphological type and on geometry of the impact. The primary result of the analysis is that the amount of gas encountered along the line of sight depends on the galaxy impact parameter and B-band luminosity but does not depend strongly on the galaxy average surface brightness, disk-to-bulge ratio, or redshift. This result confirms and improves upon an anti-correlation between Ly(alpha) absorption equivalent width and galaxy impact parameter found previously. More importantly, this result provides the first quantitative means of relating statistics of faint galaxies to statistics of Ly(alpha) absorption systems. which we plan to exploit to constrain the luminosity function of galaxies beyond the realm of current surveys. Results have been submitted for publication and will greatly improve our statistical conclusions . Together with graduate student Noriaki Yahata. I have measured and classified spectral properties of over 1000 faint galaxies and stars obtained in our low-resolution spectroscopic survey. The goal of this project is two-fold: (1) to exhaustively characterize the spectral properties of all faint galaxies that comprise our current survey, and (2) to gain experience with our measurement and classification code. which ultimately will be used on a data base of 20,000 galaxies to be obtained with the Two-Degree Field (2df) spectrograph at the Anglo-Australian Telescope (AAT). The results will ultimately be used for many goals, but so far we have concentrated on using the results to make a binary classification of the galaxies (i.e. early type versus late type) and to then exploit the density-morphology relationship to obtain a crude density indicator. The primary result of the analysis is that the incidence and extent of tenuous gas around galaxies shows no strong preference for local galaxy environment, at least over the range of densities spanned by the current observations. Along similar lines, two instances of Ly(alpha) absorption lines that arise in groups or clusters were examined. Analysis demonstrates that some can produce corresponding absorption lines and that LY(alpha) absorption lines do not avoid a high-density environment. A new measure of the galaxy-absorber cross-correlation function defines the statistical criterion by which galaxies and absorber pairs are to be matched. I have identified a damped Ly(alpha) absorption system at redshift z equals approximately 0.16, the lowest redshift confirmed to date. The most important results of the analysis are learning that the metal abundances of the absorption system are less than 10 percent of the solar metal abundance and that the absorbing gas is not rotating with the galaxy disk.

Source record↗

X-Ray Emission from the Unidentified Gamma Ray Transient GRO J1838-0415 and X-Ray Localization of Gamma-Ray Transients near the Galactic Plane

The investigation was focused on studying the residual X-ray emission from possible counterparts of enigmatic gamma-ray transients near the Galactic plane. These sources (discovered by our group at Columbia) are highly variable gamma-ray sources with no radio-loud spectrally flat blazar in their approx 1 deg. diameter error box. BSAX Narrow Field Instruments pointed the gamma-ray error box of the prominent non-blazar Energetic Gamma Ray Experiment Telescope (EGRET) source GRO 1838-04 twice on April 17, 1997, and on April 20, 1997. Most of the EGRET error box was imaged and several faint X-ray sources were detected. BSAX Wide Field Camera data were also obtained for a variety of sources near the Galactic plane, including GRO J1838-04.

Tavani, Marco↗

Genomic clocks and evolutionary timescales

For decades, molecular clocks have helped to illuminate the evolutionary timescale of life, but now genomic data pose a challenge for time estimation methods. It is unclear how to integrate data from many genes, each potentially evolving under a different model of substitution and at a different rate. Current methods can be grouped by the way the data are handled (genes considered separately or combined into a 'supergene') and the way gene-specific rate models are applied (global versus local clock). There are advantages and disadvantages to each of these approaches, and the optimal method has not yet emerged. Fortunately, time estimates inferred using many genes or proteins have greater precision and appear to be robust to different approaches.

Evolution, Molecular↗

Overexpression of IGF-I in skeletal muscle of transgenic mice does not prevent unloading-induced atrophy

This study examined the association between local insulin-like growth factor I (IGF-I) overexpression and atrophy in skeletal muscle. We hypothesized that endogenous skeletal muscle IGF-I mRNA expression would decrease with hindlimb unloading (HU) in mice, and that transgenic mice overexpressing human IGF-I (hIGF-I) specifically in skeletal muscle would exhibit less atrophy after HU. Male transgenic mice and nontransgenic mice from the parent strain (FVB) were divided into four groups (n = 10/group): 1) transgenic, weight-bearing (IGF-I/WB); 2) transgenic, hindlimb unloaded (IGF-I/HU); 3) nontransgenic, weight-bearing (FVB/WB); and 4) nontransgenic, hindlimb unloaded (FVB/HU). HU groups were hindlimb unloaded for 14 days. Body mass was reduced (P < 0.05) after HU in both IGF-I (-9%) and FVB mice (-13%). Contrary to our hypothesis, we found that the relative abundance of mRNA for the endogenous rodent IGF-I (rIGF-I) was unaltered by HU in the gastrocnemius (GAST) muscle of wild-type FVB mice. High-level expression of hIGF-I peptide and mRNA was confirmed in the GAST and tibialis anterior (TA) muscles of the transgenic mice. Nevertheless, masses of the GAST and TA muscles were reduced (P < 0.05) in both FVB/HU and IGF-I/HU groups compared with FVB/WB and IGF-I/WB groups, respectively, and the percent atrophy in mass of these muscles did not differ between FVB and IGF-I mice. Therefore, skeletal muscle atrophy may not be associated with a reduction of endogenous rIGF-I mRNA level in 14-day HU mice. We conclude that high local expression of hIGF-I mRNA and peptide in skeletal muscle alone cannot attenuate unloading-induced atrophy of fast-twitch muscle in mice.

NASA Discipline Musculoskeletal↗

Introduction to the analysis of delamination related fracture processes in composites

This research concerns the analysis and prediction of delamination damage that occurs in composite structures on the sublaminate scale - that is, the scale of individual plies or groups of plies. The objective was to develop analytical models for fixed-mode delamination in composites. These include: (1) the influence of residual thermal and moisture strains; (2) local or transverse crack tip delamination originating at the tip of transverse matrix cracks; and (3) delamination in tapered composite under tensile loading. Computer codes based on the analytical models were developed and comparisons of predictions with available experimental and analytical results in the literature were performed.

Armanios, Erian A.↗

Olympus receiver evaluation and phase noise measurements

A set of measurements performed by the Michigan Tech Sensing and Signal Processing Group on the analog receiver built by the Virginia Polytechnic Institute (VPI) and the Jet Propulsion Laboratory (JPL) for propagation measurements using the Olympus Satellite is described. Measurements of local oscillator (LO) phase noise were performed for all of the LOs supplied by JPL. In order to obtain the most useful set of measurements, LO phase noise measurements were made using the complete VPI receiver front end. This set of measurements demonstrates the performance of the receiver from the Radio Frequency (RF) input through the high Intermediate Frequency (IF) output. Three different measurements were made: LO phase noise with DC on the voltage controlled crystal oscillator (VCXO) port; LO phase noise with the 11.381 GHz LO locked to the reference signal generator; and a reference measurement with the JPL LOs out of the system.

Campbell, Richard L.↗

Pyroclastic deposits on the western limb of the moon

A geologic characterization is presented of 17 newly identified localized pyroclastic deposits on the western limb of the moon. The results show that lunar pyroclastic deposits are more pervasive on the western limb of the moon than once thought. The western limb dark-mantle deposits in the proximal zone are probably related to faults and fractures associated with the Cordillera ring of Orientale Basin. All the localized pyroclastic deposits on the western limb for which near-infrared reflectance spectra have been obtained exhibit parameter that allowed them to be assigned to the three spectral groups described elsewhere. Western limb pyroclastic deposits appear to represent various stages in a formational sequence in which an initial explosive phase is followed by varying amounts of mare flooding. In view of their sequential formation, both the pyroclastic and mare materials associated with individual volcanic complexes may be the products of the same parent magmas and have very similar compositions.

Coombs, Cassandra R.↗

Radiative transfer models

The purpose of this work was to assist with the development of analytical techniques for the interpretation of infrared observations. We have done the following: (1) helped to develop models for continuum absorption calculations for water vapor in the far infrared spectral region; (2) worked on models for pressure-induced absorption for O2 and N2 and their comparison with available observations; and (3) developed preliminary studies of non-local thermal equilibrium effects in the upper stratosphere and mesosphere for infrared gases. These new techniques were employed for analysis of balloon-borne far infrared data by a group at the Harvard-Smithsonian Center for Astrophysics. The empirical continuum absorption model for water vapor in the far infrared spectral region and the pressure-induced N2 absorption model were found to give satisfactory results in the retrieval of the mixing ratios of a number of stratospheric trace constituents from balloon-borne far infrared observations.

Horwitz, James L.↗

Transition and Turbulence Modeling for Blunt-Body Wake Flows

Aerobraking has been proposed as an efficient means of decelerating spacecraft for planetary missions. Most current aerobrake designs feature a blunt forebody shielding the payload from the intense heat generated during atmospheric entry. Although this forebody will absorb the largest portion of the heat pulse, accurate prediction of heating in the near wake is of great importance, since large local heating values can occur at points of shear-layer impingement. In order to address the various issues associated with these blunt-body wake flowfields, the Advisory Group for Aerospace Research and Development (AGARD) formed Working Group 18 in 1992. One of the objectives of this activity was to examine real-gas effects in high-speed flow fields around a 70 deg. blunted cone. To date, many researchers have conducted experiments using this geometry in various facilities, such as the Large Energy National Shock (LENS) tunnel at Cubric/Calspan and the HEG shock tunnel at DLR-Goettingen. Several computational studies have also been conducted in concert with these tests. Many of the experimental results have indicated the possible presence of a transitional shear layer through a large increase in heat transfer downstream of the reattachment point. The presence of transition could in fact lead to much higher peak heating than if the separated flow is entirely laminar or turbulent. In the shock-tunnel tests, however, it is difficult to separate such viscous-flow phenomena from real-gas effects. In order to help make this distinction, Horvath et al. recently conducted a set of experiments in the NASA Langley 20-Inch Mach 6 Tunnel, and compared the results to laminar Navier-Stokes calculations. They found heat-transfer distributions similar to those obtained in the high-enthalpy facilities, with the measured peak heating along the sting support markedly greater than that predicted by the laminar computations. These trends point to the need to find transitional and turbulent computational solutions for these flowfields.

Nance, Robert P.↗

Gibberellin (GA3) enhances cell wall invertase activity and mRNA levels in elongating dwarf pea (Pisum sativum) shoots

The invertase (EC 3.2.1.26) purified from cell walls of dwarf pea stems to homogeneity has a molecular mass of 64 kilodaltons (kD). Poly(A)+RNA was isolated from shoots of dwarf pea plants, and a cDNA library was constructed using lambda gt11 as an expression vector. The expression cDNA library was screened with polyclonal antibodies against pea cell wall invertase. One invertase cDNA clone was characterized as a full-length cDNA with 1,863 base pairs. Compared with other known invertases, one homologous region in the amino acid sequence was found. The conserved motif, Asn-Asp-Pro-Asn-Gly, is located near the N-terminal end of invertase. Northern blot analysis showed that the amount of invertase mRNA (1.86 kb) was rapidly induced to a maximal level 4 h after GA3 treatment, then gradually decreased to the control level. The mRNA level at 4 h in GA3-treated peas was fivefold higher than that of the control group. The maximal increase in activity of pea cell wall invertase elicited by GA3 occcured at 8 h after GA3 treatment. This invertase isoform was shown immunocytochemically to be localized in the cell walls, where a 10-fold higher accumulation occurred in GA3-treated tissue compared with control tissue. This study indicates that the expression of the pea shoot cell-wall invertase gene could be regulated by GA3 at transcriptional and/or translational levels.

NASA Discipline Number 40-50↗

The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy

Insulin-like growth factor-1 (IGF-1) is known to have anabolic effects on skeletal muscle cells. This study examined the time course of muscle hypertrophy and associated IGF-1 peptide and mRNA expression. Data were collected at 3, 7, 14, and 28 days after surgical removal of synergistic muscles of both normal and hypophysectomized (HX) animals. Overloading increased the plantaris (Plant) mass, myofiber size, and protein-to-body weight ratio in both groups (normal and HX; P < 0.05). Muscle IGF-1 peptide levels peaked at 3 (normal) and 7 (HX) days of overloading with maximum 4.1-fold (normal) and 6.2-fold (HX) increases. Increases in muscle IGF-1 preceded the hypertrophic response. Total DNA content of the overloaded Plant increased in both groups. There was a strong positive relationship between IGF-1 peptide and DNA content in the overloaded Plant from both groups. These results indicate that 1) the muscles from rats with both normal and severely depressed systemic levels of IGF-1 respond to functional overload with an increase in local IGF-1 expression and 2) this elevated IGF-1 may be contributing to the hypertrophy response, possibly via the mobilization of satellite cells to provide increases in muscle DNA.

NASA Discipline Musculoskeletal↗

Bringing Together Users and Developers of Forest Biomass Maps

Forests store carbon and thus represent important sinks for atmospheric carbon dioxide. Reducing uncertainty in current estimates of the amount of carbon in standing forests will improve precision of estimates of anthropogenic contributions to carbon dioxide in the atmosphere due to deforestation. Although satellite remote sensing has long been an important tool for mapping land cover, until recently aboveground forest biomass estimates have relied mostly on systematic ground sampling of forests. In alignment with fiscal year 2010 congressional direction, NASA has initiated work toward a carbon monitoring system (CMS) that includes both maps of forest biomass and total carbon flux estimates. A goal of the project is to ensure that the products are useful to a wide community of scientists, managers, and policy makers, as well as to carbon cycle scientists. Understanding the needs and requirements of these data users is helpful not just to the NASA CMS program but also to the entire community working on carbon-related activities. To that end, this meeting brought together a small group of natural resource managers and policy makers who use information on forests in their work with NASA scientists who are working to create aboveground forest biomass maps. These maps, derived from combining remote sensing and ground plots, aim to be more accurate than current inventory approaches when applied at local and regional scales. Meeting participants agreed that users of biomass information will look to the CMS effort not only to provide basic data for carbon or biomass measurements but also to provide data to help serve a broad range of goals, such as forest watershed management for water quality, habitat management for biodiversity and ecosystem services, and potential use for developing payments for ecosystem service projects. Participants also reminded the CMS group that potential users include not only public sector agencies and nongovernmental organizations but also the private sector because much forest acreage in the United States is privately held and needs data for forest management. Additional key outcomes identified by meeting participants include the following: (1) Priority should be given to building into the biomass product ease of use and low costs (including costs of hardware, software, and analysis requirements), (2) CMS products should also be relevant to other biomass measures for forest watershed management, habitat protection for biodiversity, and assessment of markets for ecosystem services, (3) CMS leadership should engage with the Subsidiary Body for Scientific and Technological Advice of the United Nations Framework Convention on Climate Change as they establish measuring, reporting, and verification standards, and (4) CMS leadership should continue to keep sister agencies and other organizations informed as CMS develops, particularly via the agencies active in the U.S. Global Change Research Program Carbon Cycle Interagency Working Group (U.S. Geological Survey, U.S. Department of Agriculture, and National Oceanic and Atmospheric Administration) and nongovernmental organizations.

Forest↗

Manicouagan impact melt, Quebec. I - Stratigraphy, petrology, and chemistry

A sheet of clast-laden impact melt 230 m thick and 55 km in diameter forms an annular plateau surrounding an uplift of shocked anorthosite within the moderately eroded Manicouagan structure. Three gradational units of the melt sheet are characterized with respect to grain size, inclusions, texture, and mineralogy. The melt rocks as a group are chemically homogeneous with a bulk composition similar to that of latite and with no statistically significant regional chemical variations. The melt is not completely chemically homogeneous as a local mafic variant represented by two samples with poikilitic texture was found. These poikilitic rocks texturally resemble some Apollo 17 impact melt rocks and are inferred to have had a similar origin and thermal history.

Floran, R. J.↗

Some properties of transient phenomena in the extra-tropical region and their importance in the seasonal mean flow

When studying data for one season, it is convenient to divide transient eddies into those of the synoptic time scale and those of lower frequency. Where the dividing line is placed is not crucial, but a 10-day period is used in a study of the 1979-1980 Northern Hemisphere winter. The synoptic time scale eddies exhibit maximum kinetic energy in stormtracks slightly downstream from and poleward of the Atlantic and Pacific jet maximum. An alternative approach is via the anisotropic part of the horizontal velocity correlation tensor, the axis of which gives the local orientation of the eddies and the magnitude a measure of the extension of the eddies. When the man velocity field is almost that of the plane waves, the horizontal group velocity relative to the mean field is also related to this tensor. The synoptic time scale eddies force the mean flow in the sense of reducing the baroclinic component in the upstream of the region of the stormtrack. The lower frequency eddies have a very different signature.

Hoskins, B. J.↗

Methods for roof-top mini-arrays

To test the idea of the Linsley effect mini array for the study of giant air showers, it is desirable to have a trigger that exploits the effect itself. In addition to the trigger, it is necessary to have a method for measuring the relative arrival times of the particle swarm selected by the trigger. Since the idea of mini arrays is likely to appeal to small research groups, it is desirable to try to design relatively simple and inexpensive methods, and methods that utilize existing detectors. Clusters of small detectors have been designed for operation in the local particle density realm where the probability of or = 2 particles per detector is small. Consequently, this method can discriminate pulses from each detector and thenceforth deal mainly with logic pulses.

Hazen, W. E.↗

Automated Wildfire Detection Through Artificial Neural Networks

We have tested and deployed Artificial Neural Network (ANN) data mining techniques to analyze remotely sensed multi-channel imaging data from MODIS, GOES, and AVHRR. The goal is to train the ANN to learn the signatures of wildfires in remotely sensed data in order to automate the detection process. We train the ANN using the set of human-detected wildfires in the U.S., which are provided by the Hazard Mapping System (HMS) wildfire detection group at NOAA/NESDIS. The ANN is trained to mimic the behavior of fire detection algorithms and the subjective decision- making by N O M HMS Fire Analysts. We use a local extremum search in order to isolate fire pixels, and then we extract a 7x7 pixel array around that location in 3 spectral channels. The corresponding 147 pixel values are used to populate a 147-dimensional input vector that is fed into the ANN. The ANN accuracy is tested and overfitting is avoided by using a subset of the training data that is set aside as a test data set. We have achieved an automated fire detection accuracy of 80-92%, depending on a variety of ANN parameters and for different instrument channels among the 3 satellites. We believe that this system can be deployed worldwide or for any region to detect wildfires automatically in satellite imagery of those regions. These detections can ultimately be used to provide thermal inputs to climate models.

Miller, Jerry↗