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At least 397 records · Page 22

Analysis of xylem formation in pine by cDNA sequencing

Secondary xylem (wood) formation is likely to involve some genes expressed rarely or not at all in herbaceous plants. Moreover, environmental and developmental stimuli influence secondary xylem differentiation, producing morphological and chemical changes in wood. To increase our understanding of xylem formation, and to provide material for comparative analysis of gymnosperm and angiosperm sequences, ESTs were obtained from immature xylem of loblolly pine (Pinus taeda L.). A total of 1,097 single-pass sequences were obtained from 5' ends of cDNAs made from gravistimulated tissue from bent trees. Cluster analysis detected 107 groups of similar sequences, ranging in size from 2 to 20 sequences. A total of 361 sequences fell into these groups, whereas 736 sequences were unique. About 55% of the pine EST sequences show similarity to previously described sequences in public databases. About 10% of the recognized genes encode factors involved in cell wall formation. Sequences similar to cell wall proteins, most known lignin biosynthetic enzymes, and several enzymes of carbohydrate metabolism were found. A number of putative regulatory proteins also are represented. Expression patterns of several of these genes were studied in various tissues and organs of pine. Sequencing novel genes expressed during xylem formation will provide a powerful means of identifying mechanisms controlling this important differentiation pathway.

Non-NASA Center↗

Nuclear size as a cell-kinetic marker for osteoblast differentiation

A nuclear morphometric assay for preosteoblasts is introduced as a cell-kinetic technique, applicable to routine histological preparations of mineralized tissue. Because this method is a morphological marker for osteoblast precursor cell differentiation, it provides a new dimension for determining the mechanism of osteoblast histogenesis. Osteoblast precursors of the periodontal ligament are a mixed population of progenitors, kinetically separable into two distinct groups according to nuclear size. Preosteoblasts, the immediate proliferating precursors of osteoblasts, have large nuclei (greater than 170 micrometers3) and are derived from relatively undifferentiated fibroblastlike cells, which have smaller nuclei (less than 80 micrometers3). Increase in nuclear volume, during G1 phase of the cell cycle, is apparently a morphological manifestation of change in genomic expression. This key event in preosteoblast differentiation is related to mechanical stress/strain and may be an important rate-limiting step in osteoblast histogenesis.

Non-NASA Center↗

Effects of Hydrogen on GRCop-84

This report is a section of the final report on the GRCop-84 task of the Constellation Program and incorporates the results obtained between October 2000 and September 2005 when the program ended. GRCop-84 contains approximately 5.5 wt% Nb. Nb can react with H and embrittle easily. Previous work had indicated the thermodynamic possibility that Cr 2 Nb could react with H and form niobium hydrides in the presence of high pressure H such as seen in the Space Shuttle Main Engine. In this study, samples were charged with H and then tested in both high pressure H and He environments to determine if measurable differences existed which indicate that hydrogen embrittlement occurs in GRCop-84. Tensile, notched tensile, stress rupture and low cycle fatigue properties were surveyed. High pressure H environment stress rupture testing resulted in a lower reduction in area than a high pressure He environment, and the LCF lives at high strain ranges fall below the lower 95 percent confidence interval for the baseline data, but in general no significant differences were noted either between H and He environment tests or between hydrogen charged materials and the baseline, uncharged extruded GRCop-84 data sets. There was also no discernable evidence of the formation of hydrides or changes in fracture morphology indicating hydrogen embrittlement occurred.

Ellis, David L.↗

Comparison of the Atomic Oxygen Erosion Depth and Cone Height of Various Materials at Hyperthermal Energy

Atomic oxygen readily reacts with most spacecraft polymer materials exposed to the low Earth orbital (LEO) environment. If the atomic oxygen arrival comes from a fixed angle of impact, the resulting erosion will foster the development of a change in surface morphology as material thickness decreases. Hydrocarbon and halopolymer materials, as well as graphite, are easily oxidized and textured by directed atomic oxygen in LEO at energies of approx.4.5 eV. What has been curious is that the ratio of cone height to erosion depth is quite different for different materials. The formation of cones under fixed direction atomic oxygen attack may contribute to a reduction in material tensile strength in excess of that which would occur if the cone height to erosion depth ratio was very low because of greater opportunities for crack initiation. In an effort to understand how material composition affects the ratio of cone height to erosion depth, an experimental investigation was conducted on 18 different materials exposed to a hyperthermal energy directed atomic oxygen source (approx.70 eV). The materials were first salt-sprayed to provide microscopic local areas that would be protected from atomic oxygen. This allowed erosion depth measurements to be made by scanning microscopy inspection. The polymers were then exposed to atomic oxygen produced by an end Hall ion source that was operated on pure oxygen. Samples were exposed to an atomic oxygen effective fluence of 1.0x10(exp 20) atoms/sq cm based on Kapton H polyimide erosion. The average erosion depth and average cone height were determined using field emission scanning electron microscopy (FESEM). The experimental ratio of average cone height to erosion depth is compared to polymer composition and other properties.

Waters, Deborah L.↗

The 2006-2007 Active Phase Of Anomalous X-Ray Pulsar 4U 0142+61: Radiative and Timing Changes, Bursts, and Burst Spectral Features

After at least 6 years of quiescence, Anomalous X-ray Pulsar (AXP) 4U 0142+61 entered an active phase in 2006 March that lasted several months and included six X-ray bursts as well as many changes in the persistent X-ray emission. The bursts, the first seen from this AXP in >11 years of Rossi X-ray Timing Explorer monitoring, all occurred in the interval between 2006 April 6 and 2007 February 7. The burst durations ranged from 8-3x10(exp 3)s. The first five burst spectra are well modeled by blackbodies, with temperatures kT approx. 2 - 6 keV. However, the sixth burst had a complicated spectrum that is well characterized by a blackbody plus three emission features whose amplitude varied throughout the burst. The most prominent feature was at 14.0 keV. Upon entry into the active phase the pulsar showed a significant change in pulse morphology and a likely timing glitch. The glitch had a total frequency jump of (1.9+/-0.4)x10(exp -7) Hz, which recovered with a decay time of 17+/-2 days by more than the initial jump, implying a net spin-down of the pulsar. We discuss these events in the context of the magnetar model.

Gavril, Fotis P.↗

Method to Improve Indium Bump Bonding via Indium Oxide Removal Using a Multi-Step Plasma Process

A process for removing indium oxide from indium bumps in a flip-chip structure to reduce contact resistance, by a multi-step plasma treatment. A first plasma treatment of the indium bumps with an argon, methane and hydrogen plasma reduces indium oxide, and a second plasma treatment with an argon and hydrogen plasma removes residual organics. The multi-step plasma process for removing indium oxide from the indium bumps is more effective in reducing the oxide, and yet does not require the use of halogens, does not change the bump morphology, does not attack the bond pad material or under-bump metallization layers, and creates no new mechanisms for open circuits.

Greer, H. Frank↗

Three-Dimensional Distribution of Ejecta in Supernova 1987A at 10,000 Days

Due to its proximity, SN 1987A offers a unique opportunity to directly observe the geometry of a stellar explosion as it unfolds. Here we present spectral and imaging observations of SN 1987A obtained approximately 10,000 days after the explosion with HST/STIS and VLT/SINFONI at optical and near-infrared wavelengths. These observations allow us to produce the most detailed 3D map of H(alpha) to date, the first 3D maps for [Ca II] lambda lambda 7292, 7324 [O I] lambda lambda 6300, 6364, and Mg II lambda lambda 9218, 9244, as well as new maps for [Si I] + [Fe II] 1.644 micrometers and He I 2.058 micrometers. A comparison with previous observations shows that the [Si I] + [Fe II] flux and morphology have not changed significantly during the past ten years, providing evidence that this line is powered by Ti-44. The time evolution of H(alpha) shows that it is predominantly powered by X-rays from the ring, in agreement with previous findings. All lines that have sufficient signal show a similar large-scale 3D structure, with a north-south asymmetry that resembles a broken dipole. This structure correlates with early observations of asymmetries, showing that there is a global asymmetry that extends from the inner core to the outer envelope. On smaller scales, the two brightest lines, Hα and [Si I]+[Fe II] 1.644 micrometers, show substructures at the level of approximately 200-1000 km s(exp -1) and clear differences in their 3D geometries. We discuss these results in the context of explosion models and the properties of dust in the ejecta.

Larsson, J.↗

E-Beam Generated Plasma Etching for Developing High-Reflectance Mirrors for Far-Ultraviolet Astronomical Instrument Applications

Astronomical space telescopes to study astrophysical phenomena from the far-ultraviolet (FUV) to the near infrared (NIR) will require mirror coatings with high reflectance over this entire spectral region. While coatings for the optical and NIR part of the spectrum are fairly well developed with proven performance, the FUV presents significant challenges. The U.S. Naval Research Laboratory (NRL) has developed a processing system based on an electron beam-generated plasma that provides for controlled fluorination and/or etching of surfaces with near monolayer precision and minimal changes to surface morphology. In this paper, we report recent results of samples treated in the NRL Large Area Plasma Processing System (LAPPS) where restoration of the high intrinsic reflectance in the FUV spectral range have been observed of aluminum (Al) mirrors protected with a magnesium di-fluoride (MgF2) overcoat. This paper will also extend these studies to other Al mirrors protected with aluminum tri-fluoride (AlF3) in order to realize the high intrinsic reflectance Al down to FUV wavelengths (100−200 nm), while still maintaining the high reflectance in the optical and NIR spectral regions. Laboratory test data and optical diagnostic techniques used to verify surface scattering and durability of selected coatings will be presented. Finally, we will discuss the scalability of the LAPPS etching process in order to realize these high-reflectivity coatings on mirror segments as large as those proposed for the Large Ultraviolet, Optical, and Infrared (LUVOIR) astronomical telescope system (1+meter class).

LUVOIR↗

The Effects of CDKN1a/p21 on Oxidative Stress and Mitochondrial Function During Long Duration Spaceflight

Spaceflight factors, such as microgravity and space radiation, are known to put astronauts at risk for negative effects to their tissue regeneration and degenerative conditions. On our previous spaceflight research, we found that long duration spaceflight produced increased oxidative stress and activation of the inflammatory response as a major contributor to physiological changes, including alterations to the cardiovascular and musculoskeletal systems, metabolism, and the liver. Proliferation, apoptosis, and ion-channel remodeling of vascular smooth muscle cells, as well as endothelial inflammation, and nitric oxide synthase-nitric oxide system regulation have been shown by spaceflight and simulated spaceflight projects. CDKN1ap21 is a regulatory gene for tissue regeneration and also plays an important role in upregulating oxidative stress caused by radiation. In further research, we have also determined CDKN1ap21 to be overexpressed during spaceflight. When knocked out in mice, however, it protects against unloading-induced changes to the morphology and physiology of carotid arteries. In this proposal, we seek to test a potential dietary countermeasure to down-regulate CDKN1ap21 in the mice and protect against oxidative stress in the rodents. Specifically, we will expose both Wildtype and CDKN1ap21-null mice to hydrogen peroxide to induce oxidative stress and observe the molecular mechanisms in which a dietary supplement protects vascular smooth muscle cells against the consequences of the apoptotic environment within Wildtype mice versus the, hypothetically, sound physiology of CDKN1ap21-null mice. The successful completion of this experiment will conclusively determine accuracy of a dietary supplement as a CDKN1ap21 suppressor. This will provide a new perspective on the problem of increased oxidative stress developing into cardiovascular disease as a result of long-term space flight. This research will address the Human Research Plan Roadmaps Risk of Cardiac Rhythm Problems including CV-8, the Risk of Cardiovascular Disease and Other Degenerative Tissue Effects from Radiation Exposure including Degen-6 and Degen-7, and the Risk of Adverse Health Event from Radiation Exposure including IM-8. Additionally, this research will address Space Biology Research Plans questions SBP CMB-1, SBP CMB-3, and SB AN-2.

Oxidative Stress↗

Decadal Changes in Barrier Island Facies near Mansfield Pass on Padre Island, Texas from 1950-Present

Barrier islands serve an important role in shielding coastal areas from storms and wave erosion. Monitoring changes in barrier island systems helps understand the effect of storms and sea level rise on the stability of the island. The study area is at Padre Island, near Mansfield Pass, has seen wide-spread changes in surface morphology underpinned by significant growth of vegetation and microbial mats.

Kenton R. Fisher↗

Radiative properties of advanced spacecraft heat shield materials

Experimental results are presented to show the effects of simulated reentry exposure by convective heating and by radiant heating on spectral and total emittance of statically oxidized Inconel 617 and Haynes HS188 superalloys to 1260 K and a silicide coatea (R512E) columbium 752 alloy to 1590 K. Convective heating exposures were conducted in a supersonic arc plasma wind tunnel using a wedge-shaped specimen configuration. Radiant tests were conducted at a pressure of .003 atmospheres of dry air at a flow velocity of several meters per second. Convective heating specimens were subjected to 8, 20, and 38 15-min heating cycles, and radiant heating specimens were tested for 10, 20, 50, and 100 30-min heating cycles. Changes in radiative properties are explained in terms of changes in composition resulting from simulated reentry tests. The methods used to evaluate morphological, compositional and crystallographic changes include: Auger electron spectroscopy; scanning electron microscopy; X-ray diffraction analysis; and electron microprobe analysis.

Cunnington, G. R.↗

Viscoelastic characterization of thin-film polymers exposed to low Earth orbit

The materials made available through the Long Duration Exposure Facility (LDEF) satellite provide a set of specimens that can be well characterized and have a known exposure history with reference to atomic oxygen and ultraviolet radiation exposure. Mechanical characteristics measured from control samples and exposed samples provide a data base for predicting the behavior of polymers in low earth orbit. Samples of 1.0 mil thick low density polyethylene were exposed to the low earth orbit environment for a period of six years. These materials were not directly exposed to ram atomic oxygen and offer a unique opportunity for measuring the effect of atomic oxygen and UV radiation on mechanical properties with little concern to the effect of erosion. The viscoelastic characteristics of these materials were measured and compared to the viscoelastic characteristics of control samples. To aid in differentiating the effects of changes in crystallinity resulting from thermal cycling, from the effects of changes in chemical structure resulting from atomic oxygen/UV attack to the polymer, a second set of control specimens, annealed to increase crystallinity, were measured as well. The resulting characterization of these materials will offer insight into the impact of atomic oxygen/UV on the mechanical properties of polymeric materials. The viscoelastic properties measured for the control, annealed, and exposed specimens were the storage and loss modulus as a function of frequency and temperature. From these datum is calculated the viscoelastic master curve derived using the principle of time/temperature superposition. Using the master curve, the relaxation modulus is calculated using the method of Ninomiya and Ferry. The viscoelastic master curve and the stress relaxation modulus provide a direct measure of the changes in the chemical or morphological structure. In addition, the effect of these changes on long-term and short-term mechanical properties is known directly. It should be noted that the dependence on directionality for the polymer films was considered since these films were manufactured by a blown-film process.

Letton, Alan↗

VISUALIZING INTERANNUAL TO DECADAL COASTAL VARIABILITY NEAR VULNERABLE NASA INFRASTRUCTURE UTILIZING VIDEOS PRODUCED FROM REMOTELY SENSED DATA AT CAPE CANAVERAL, FL AND WALLOPS ISLAND, VA

Coastlines represent an interesting convolution of intense anthropogenic development superimposed onto morphologies that are among the most dynamic on the planet. Two of NASA’s critical facilities are particularly vulnerable to coastal change, Kennedy Space Center, Cape Canaveral, FL and Wallops Flight Facility, Wallops Island, VA. With continuing global sea level rise and increasing storminess coastal vulnerabilities at these facilities will only be exacerbated. These launch centers a subject to multiple coastal hazards including, flooding, episodic coastal erosion, saltwater intrusion, and coastal morphological changes. Although rapid change is readily observed during large storms or hurricanes, long term annual to decadal coastal change is much harder to visualize and conceptualize. The authors use videos developed from remotely sensed data to enhance conceptualization of coastal processes for planning and management stakeholders at these NASA facilities. Near or better than decadal coastal imagery has been available through high altitude photogrammetry efforts since the 1930’s. A significant amount of additional imagery was acquired through other federal, state, and local government agencies such as the USGS, NOAA, State DOT, and local Assessors Offices providing annual and inter annual images at spatial resolutions of 10cm – 1m. With the advent of GIS systems these images could be rectified and then layered to better understand change through time using a multitude of visual base shoreline proxies. With the increased awareness and use of video editing software the rectified images can be used to create videos. These videos can then be used to conceptualize a multitude of coastal processes including: performance of coastal restoration efforts, rapid change in sedimentation rates due to storms or other geomorphic processes, loss of protective barrier islands as sea level rises and lower sedimentation rates occur. The videos are also useful as a tool for verification and comparison to coastal models and provide an easy to understand educational resource for stakeholder engagement at these NASA centers, and other vulnerable coastal populations.

Richard A. MacKenzie III↗

Modeling Radiation-Induced Epithelial Cell Injury in Murine Three-Dimensional Esophageal Organoids

Esophageal squamous cell carcinoma (ESCC) is a deadly consequence of radiation exposure to the esophagus. ESCC arises from esophageal epithelial cells that undergo malignant transformation and features a perturbed squamous cell differentiation program. Understanding the dose- and radiation quality-dependence of the esophageal epithelium response to radiation may provide insights into the ability of radiation to promote ESCC. We have explored factors that may play a role in esophageal epithelial radiosensitivity and their potential relationship to ESCC risk. We have utilized a murine three-dimensional (3D) organoid model that recapitulates the morphology and functions of the stratified squamous epithelium of the esophagus to study persistent dose- and radiation quality-dependent changes. Interestingly, although high-linear energy transfer (LET) Fe ion exposure induced a more intense and persistent alteration of squamous differentiation and 53BP1 DNA damage foci levels as compared to Cs, the MAPK/SAPK stress pathway signaling showed similar altered levels for most phospho-proteins with both radiation qualities. In addition, the lower dose of high-LET exposure also revealed nearly the same degree of morphological changes, even though only ~36% of the cells were predicted to be hit at the lower 0.1 Gy dose, suggesting that a bystander effect may be induced. Although p38 and ERK/MAPK revealed the highest levels following high-LET exposure, the findings reveal that even a low dose (0.1 Gy) of both radiation qualities can elicit a persistent stress signaling response that may critically impact the differentiation gradient of the esophageal epithelium, providing novel insights into the pathogenesis of radiation-induced esophageal injury and early stage esophageal carcinogenesis.

3D organoid culture↗

In situ Gas Cell TEM Investigation of Nanoporous and Zeolite-based Nanocatalysts

A crucial application of in situ transmission electron microscopy is the understanding of nanocatalysts and the effect of the environment on their structure. Pre-treatments, such as calcination or annealing, can dramatically impact the catalytic properties by modifying the morphology and composition of nanostructures. Zeolites and nanoporous structures are particularly sensitive to reactive environments as the porosity or the chemical state can substantially change during the interaction with gases at elevated temperatures.[1] For instance, it has been shown that heating in air causes redispersion of sintered Cu clusters on zeolite, improving the catalytic properties.[2] These observations are possible though innovative in situ TEM gas holders, where the samples are enclosed into a small cell with SiNx windows, isolating the reactive environment from the rest of the column. [3] Here, we present in situ gas cell experiments of sensitive nanoporous structure and zeolite-based nanocatalysts. Scanning transmission electron microscopy (STEM) and electron energy-loss spectroscopy were used to understand migration of Al during calcination. In situ diagnostics also help distinguish Al as Bronsted sites, extra-framework Lewis sites, or bulk alumina. More broadly, in situ gas-heating TEM experiments are useful to determine chemical changes and modification of morphology of sensitive nanocatalysts upon pre-treatment (Figure 1).[4] Detailed in situ STEM and energy-dispersive X-ray spectroscopy (EDS) demonstrated compositional changes in nanoporous CuAlTi structures for hydrogen-deuterium exchange (H2-D2) reaction. Coarsening due to annealing at high temperature, a necessary steps for the preparation of catalysts, can be reversed by applying a redox cycle. Using the wide range of gases and temperature, the diagnostics are helpful to derive fundamental understanding of these catalysts at the atomic scale and also provide general guidelines to improve their design.

Foucher, Alexandre [ORNL] (ORCID:0000000150424002)↗

Adherent Al2O3 scales produced on undoped NiCrAl alloys

Repeated oxidation and polishing of high purity Ni-15Cr-13Al has dramatically changed its cyclic oxidation behavior from nonadherent to adherent. No apparent change in scale phase, morphology or interface structure occurred during this transition, dismissing any mechanism based on pegging, vacancy sink, or growth stress. The principle change that did occur was a reduction in the sulfur content from 10 ppmw to 3 ppmw after 25 cycles at 1120 C. These observations are used to support the model of Al2O3 scale adherence put forth by Smeggil et al. which claims that Al2O3 scale spallation occurs due to sulfur segregation and bond deterioration at the oxide-metal interface.

Smialek, James L.↗

High-resolution optical imaging of the 'Frosty Leo' nebula

High-resolution optical images have been obtained of IRAS 09371 + 1212 (the 'Frosty Leo' nebula). The images were taken with the Dominion Astrophysical Observatory/Canada-France-Hawaii Telescope (DAO/CFHT) High-Resolution Camera using broadband V and I filters and with an intermediate-band H-alpha filter. The red filters, particularly H-alpha show that the bipolar nebula is surrounded by a nearly spherical envelope of material approximately 30 sec in diameter. The high-resolution images allow a detailed study of changes in the nebular morphology with wavelength. The ansae remain stationary with wavelength, whereas the positions of the lobes and the position angle of the disk change with wavelength from the optical to the near infrared. These results suggest that the ejection process is colliminated by the disk, which has been processing with time. The possibility that the Frosty Leo nebula is not a post-asymptotic giant branch (AGB) star but a pre-main-sequence object formed in isolation from interstellar clouds is discussed.

Langill, Philip P.↗