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Near-Field Scanning Optical Microscopy of Soft, Biological, or Rough Objects in Aqueous Environment: Challenges and some Remedies to Circumvent

Near-field scanning optical microscopy is an established technique for sub-wavelength spatial resolution in imaging, spectroscopy, material science, surface chemistry, polarimetry, etc. A significant amount of confidence has been established for thin hard specimens in air. However when soft, biological, rough, in aqueous environment object, or a combination is involved, the progress has been slow. The tip-sample mechanical interaction, heat effects to sample, drag effects to the probe, difficulty in controlling tip-sample separation in case of rough objects, light scattering from sample thickness, etc. create problems. Although these problems are not even fully understood, there have been attempts to study them with the aim of performing reliable operations. In this review we describe these attempts. Starting with general problems encountered, various effects like polarization, thermal, and media are covered. The roles of independent tip-sample distance control tools in the relevant situations are then described. Finally progress in fluid cell aspect has been summarized.

Vikram, C. S.↗

The Athena Mars Rover Science Payload

The Mars Surveyor missions that will be launched in April of 2001 will include a highly capable rover that is a successor to the Mars Pathfinder mission's Sojourner rover. The design goals for this rover are a total traverse distance of at least 10 km and a total lifetime of at least one Earth year. The rover's job will be to explore a site in Mars' ancient terrain, searching for materials likely to preserve a record of ancient martian water, climate, and possibly biology. The rover will collect rock and soil samples, and will store them for return to Earth by a subsequent Mars Surveyor mission in 2005. The Athena Mars rover science payload is the suite of scientific instruments and sample collection tools that will be used to perform this job. The specific science objectives that NASA has identified for the '01 rover payload are to: (1) Provide color stereo imaging of martian surface environments, and remotely-sensed point discrimination of mineralogical composition. (2) Determine the elemental and mineralogical composition of martian surface materials. (3) Determine the fine-scale textural properties of these materials. (4) Collect and store samples. The Athena payload has been designed to meet these objectives. The focus of the design is on field operations: making sure the rover can locate, characterize, and collect scientifically important samples in a dusty, dirty, real-world environment. The topography, morphology, and mineralogy of the scene around the rover will be revealed by Pancam/Mini-TES, an integrated imager and IR spectrometer. Pancam views the surface around the rover in stereo and color. It uses two high-resolution cameras that are identical in most respects to the rover's navigation cameras. The detectors are low-power, low-mass active pixel sensors with on-chip 12-bit analog-to-digital conversion. Filters provide 8-12 color spectral bandpasses over the spectral region from 0.4 to 1.1 micron Narrow-angle optics provide an angular resolution of 0.28 mrad/pixel, nearly a factor of four higher than that of the Mars Pathfinder and Mars Surveyor '98 cameras. Image compression will be performed using a wavelet compression algorithm. The Mini-Thermal Emission Spectrometer (Mini-TES) is a point spectrometer operating in -the thermal IR. It produces high spectral resolution (5 /cm) image cubes with a wavelength range of 5-40 gm, a nominal signal/noise ratio of 500:1, and a maximum angular resolution of 7 mrad (7 cm at a distance of 10 in). The wavelength region over which it operates samples the diagnostic fundamental absorption features of rockforming minerals, and also provides some capability to see through dust coatings that could tend to obscure spectral features. The mineralogical information that Mini-TES provides will be used to select from a distance the rocks and soils that will be investigated in more detail and ultimately sampled. Mini-TES is derived from the MO/MGS TES instrument, but is significantly smaller and simpler. The instrument uses an 8-cm Cassegrain telescope, a Michelson interferometer, and uncooled pyroelectric detectors. Along with its mineralogical capabilities, Mini-TES can provide information on the thermophysical properties of rocks and soils. Viewing upward, it can also provide temperature profiles through the martian atmospheric boundary layer. Elemental and Mineralogical Composition: Once promising samples have been identified from a distance using Pancam/Mini-TES, they will be studied in detail using up to three compositional sensors that can be placed directly against them by an Instrument Arm. The two compositional sensors, presently on the payload are an Alpha-Proton-X-Ray Spectrometer (APXS), and a Mossbauer Spectrometer. The APXS is derived closely from the instrument that flew on Mars Pathfinder. Radioactive alpha sources and three detection modes (alpha, proton, and x-ray) provide elemental abundances of rocks and soils to complement and constrain mineralogical data. The Athena APXS will have a revised mechanical design that will cut down significantly on backscattering of alpha particles from martian atmospheric carbon. It will also include a target of known elemental composition that will be used for calibration purposes. The Athena Mossbauer Spectrometer is a diagnostic instrument for the mineralogy and oxidation state of Fe-bearing phases, which are particularly important on Mars. The instrument measures the resonant absorption of gamma rays produced by a Co-57 source to determine splitting of nuclear energy levels in Fe atoms that is related to the electronic environment surrounding them. It has been under development for space flight for many years at the Technical University of Darmstadt. The Mossbauer Spectrometer (and the other arm instruments) will be able to view a small permanent magnet array that will attract magnetic particles in the martian soil. The payload may also include a Raman Spectrometer. If included, the Raman Spectrometer will provide precise identification of major and minor mineral phases. It requires no sample preparation, and is also sensitive to organics. Fine-Scale Texture: The Instrument Arm a also carries a Microscopic Imager that will obtain high-resolution monochromatic images of the same materials for which compositional data will be obtained. Its spatial resolution is 20 micron/pixel over a 1 cm depth of field, and 40 micron/pixel over a 1-cm depth of field. Like Pancam, it uses the same active pixel sensor detectors and electronics as the rover's navigation cameras. The Instrument Arm is a three degree-of-freedom arm that uses designs and components from the Mars Pathfinder and Mars Surveyor '98 projects. Its primary function is instrument positioning. Along with the instruments noted above, it also carries a brush that can be used to remove dust and other loose coatings from rocks. Sample Collection and Storage: Martian rock and soil samples will be collected using a low-power rotary coring drill called the Mini-Corer. An important characteristic of this device is that it can obtain intact samples of rock from up to 5 cm within strong boulders and bedrock, Nominal core dimensions are 8xl7 mm. The Mini-Corer drills a core to the commanded depth in a rock, shears it off, retains it, and extracts it. It can also acquire samples of loose soil, using soil sample cups that are pressed downward into loose material. The Mini-Corer can drill at angles from vertical to 45' off vertical. It has six interchangeable bits for long life. Mechanical damage to the sample during drilling is minimal, and heating is negligible. After acquisition, the sample may be viewed by the arm instruments, and/or placed in one of 104 compartments in the Sample Container. A subset of the acquired samples may be replaced with other samples obtained later if desired. The Sample Container has no moving parts, and is mounted external to the rover for easy removal by the Mars Surveyor 2005 flight system. Operation of the rover will make extensive use of automated onboard navigation and hazard avoidance capabilities. Otherwise, use of onboard autonomy is minimal. Data downlink capability is about 40 Mbit/sol, and the use of the Mars Surveyor '01 orbiter for data relay imposes a limit of at most two command cycles per sol. Because of the significant amount of time available between command cycles, all payload elements will be operated sequentially, rather than in parallel.; this approach also significantly simplifies operations and minimizes peak power usage. The landing site for the '01 rover has not been selected yet. Site selection will make as full use as possible of Mars Global Surveyor data, and will involve substantial input from the broad Mars science community. Summary: The following table describes the mass, power, providers, and key scientific objectives of all the major elements of the Athena payload. Additional Athena payload information may be found at: http://astrosun.tn.cornell.edu/athena/index.html. Additional information contained in the original.

Squyes, S. W.↗

Materials for Shielding Astronauts from the Hazards of Space Radiations

One major obstacle to human space exploration is the possible limitations imposed by the adverse effects of long-term exposure to the space environment. Even before human spaceflight began, the potentially brief exposure of astronauts to the very intense random solar energetic particle (SEP) events was of great concern. A new challenge appears in deep space exploration from exposure to the low-intensity heavy-ion flux of the galactic cosmic rays (GCR) since the missions are of long duration and the accumulated exposures can be high. Because cancer induction rates increase behind low to rather large thickness of aluminum shielding according to available biological data on mammalian exposures to GCR like ions, the shield requirements for a Mars mission are prohibitively expensive in terms of mission launch costs. Preliminary studies indicate that materials with high hydrogen content and low atomic number constituents are most efficient in protecting the astronauts. This occurs for two reasons: the hydrogen is efficient in breaking up the heavy GCR ions into smaller less damaging fragments and the light constituents produce few secondary radiations (especially few biologically damaging neutrons). An overview of the materials related issues and their impact on human space exploration will be given.

Wilson, J. W.↗

Materials of the final reports on the joint Soviet-American experiment on the Kosmos-936 biosatellite

Biological experiments onboard the Kosmos-936 investigated the effect of weightlessness on the basic components of cells, the genetic structure and energy apparatus. Genetic studies were made on the Drosophila melanogaster. Experiments were made on higher vegetation and fungi as well. The results indicate that weightlessness cannot be the principal barrier for normal development. An experiment with ectopic osteogenesis in weightlessness was carried out. Measurements were made of cosmic radiation inside and outside the biosatellite.

Timofeyev-Resolskiy, N. V.↗

Effect of Radiation on Biologically Active Glasses

Multifunctional hydroxyapatites single crystals have been studied for their applications as the laser host material since past several decades. It is only recently their potential has been evaluated for bioactive materials. In the past researches, Czochralski and flux growth methods have been utilized to achieve single crystals. We have used low temperature processing techniques for synthesis. Organic melt was used to achieve oriented fibers by the directional solidification method. This organic treated material has different characteristics than coarsened oxide materials. Our approach involved low temperature processing using nano engineered powders of the material system Na2O-K2O-CaO- MgO-SrO-SiO2. Also, borates were processed by sintering and grain growth. Effect of -ray was studied by measuring the electrical characteristics of radiated samples. Our experiments to further improve mechanical characteristics indicate that substitution of calcium with some other elements such as gallium have great potential to improve the radiation hardening and mechanical properties of bones.

Tufail, Areeba↗

USSR Space Life Sciences Digest, issue 9

This is the ninth issue of NASA's USSR Space Lifes Sciences Digest. It contains abstracts of 46 papers recently published in Russian language periodicals and bound collections and of a new Soviet monograph. Selected abstracts are illustrated with figures and tables from the original. Additional features include reviews of a Russian book on biological rhythms and a description of the papers presented at a conference on space biology and medicine. A special feature describes two paradigms frequently cited in Soviet space life sciences literature. Information about English translations of Soviet materials available to readers is provided. The abstracts included in this issue have been identified as relevant to 28 areas of aerospace medicine and space biology. These areas are: adaptation, biological rhythms, body fluids, botany, cardiovascular and respiratory systems, developmental biology, endocrinology, enzymology, equipment and instrumentation, gastrointestinal system, genetics, habitability and environment effects, hematology, human performance, immunology, life support systems, mathematical modeling, metabolism, microbiology, morphology and cytology, musculoskeletal system, nutrition, neurophysiology, operational medicine, perception, personnel selection, psychology, radiobiology, and space biology and medicine.

Hooke, Lydia Razran↗

Study on the biological effect of cosmic radiation and the development of radiation protection technology (L-11)

NASDA is now participating in a series of flight experiments on Spacelab missions. The first experiment was carried out on the first International Microgravity Laboratory Mission (IML-1) January 1992, and the second experiment will be conducted on the Spacelab-J Mission, First Materials Processing Test (FMPT). The equipment or Radiation Monitoring Container Devices (RMCD) includes passive dosimeter systems and biological specimens. The experiments using this hardware are designed by NASDA to measure and investigate the radiation levels inside spacecraft like space shuttle and to look at the basic effects of the space environment from the aspect of radiation biology. The data gathered will be analyzed to understand the details of biological effects as well as the physical nature of space radiation registered in the sensitive Solid-State Track Detectors (SSTD).

Nagaoka, Shunji↗

Characterization of secondary ignition sources in unattended compartments and full-scale baseline test

The characteristics of five fuel loads burned within a metal lavatory were identified. In 15 of the tests the lavatory door remained closed for the 30-minute test period while in 15 additional tests the door was opened after the fire had developed. Upon completion of these tests the most severe source was selected for use in the baseline test. In the baseline test, the lavatory and adjacent panels, all of which were constructed of contemporary materials, were tested for a period of 1 hour. Thermal, environmental, and biological data were obtained for all fuel loads, door conditions, and the baseline test. All tests were conducted in a cabin fire simulator with separate ventilation of the cabin and lavatory representative of an inflight condition. The baseline test established that by using the most severe fuel source: (1) the exposed animal subject survived without complications; (2) no toxic levels of gas within the cabin were detected; (3) a propagating fire did not develop in adjacent structures; (4) the lavatory containing the fire remained structurally intact; (5) decomposition of portions of the lavatory did occur; and (6) cabin visibility would have presented a problem after 5 minutes.

Klink, D. M.↗

Compact X-Ray And Extreme-Ultraviolet Monochromator

Monochromator for x-ray and extreme-ultraviolet radiation provides higher spectral resolution than achieved with thin-metal-foil broad-band-pass filters. Uses Bragg reflection to band-pass-filter radiation from high-intensity, broad-spectrum source. Operates over much broader x-ray and extreme-ultraviolet spectrum than devices based upon natural crystals. Intended to filter continuum radiation from sources like synchrotrons, laser plasma sources, free-electron lasers, and wigglers to produce monochromatic beams for testing and analysis of x-ray and extreme-ultraviolet telescopes and microscopes, for calibration of photographic films and detectors, for biological and biomedical research, for x-ray lithography, for processing of materials, and for research in properties of materials.

Hoover, Richard B.↗

Ultra-High-Spectral-Resolution X-Ray/EUV Monochromator

Ultra-high-spectral-resolution X-Ray/EUV monochromator depends on Bragg reflection from multilayer coats and diffraction by mutlilayer-coated grating to select narrow wavelength band from input beam. Its monochromatic output beam of accurately known wavelength very useful in testing and calibration of x-ray telescopes, x-ray microscopes, photographic films, and photodetectors; in research in biological and biomedical disciplines, x-ray crystallography, properties and processing of materials, and x-ray lasers; and in x-ray lithography.

Hoover, Richard B.↗

Deep Space Test Bed for Radiation Studies

The Deep Space Test-Bed (DSTB) Facility is designed to investigate the effects of galactic cosmic rays on crews and systems during missions to the Moon or Mars. To gain access to the interplanetary ionizing radiation environment the DSTB uses high-altitude polar balloon flights. The DSTB provides a platform for measurements to validate the radiation transport codes that are used by NASA to calculate the radiation environment within crewed space systems. It is also designed to support other Exploration related investigations such as measuring the shielding effectiveness of candidate spacecraft and habitat materials, testing new radiation monitoring instrumentation and flight avionics and investigating the biological effects of deep space radiation. We describe the work completed thus far in the development of the DSTB and its current status.

Adams, James H.↗

Overview of Mars Science Laboratory (MSL) Environmental Program

This viewgraph presentation is an overview of the Mars Science Laboratory (MSL) program. The engineering objectives of the program are to create a Mobile Science Laboratory capable of one Mars Year surface operational lifetime (670 Martian sols = 687 Earth days). It will be able to land and operation over wide range of latitudes, altitudes and seasons It must have controlled propulsive landing and demonstrate improved landing precision via guided entry The general science objectives are to perform science that will focus on Mars habitability, perform next generation analytical laboratory science investigations, perform remote sensing/contact investigations and carry a suite of environmental monitoring instruments. Specific scientific objectives of the MSL are: (1) Characterization of geological features, contributing to deciphering geological history and the processes that have modified rocks and regolith, including the role of water. (2) Determination of the mineralogy and chemical composition (including an inventory of elements such as C, H, N, O, P, S, etc. known to be building blocks for life) of surface and near-surface materials. (3) Determination of energy sources that could be used to sustain biological processes. (4) Characterization of organic compounds and potential biomarkers in representative regolith, rocks, and ices. (5) Determination the stable isotopic and noble gas composition of the present-day bulk atmosphere. (6) Identification potential bio-signatures (chemical, textural, isotopic) in rocks and regolith. (7) Characterization of the broad spectrum of surface radiation, including galactic cosmic radiation, solar proton events, and secondary neutrons. (8) Characterization of the local environment, including basic meteorology, the state and cycling of water and C02, and the near-surface distribution of hydrogen. Several views of the planned MSL and the rover are shown. The MSL environmental program is to: (1) Ensure the flight hardware design is capable of surviving all the environments throughout its mission life time, including ground, transportation, launch, cruise, entry decent and landing (EDL) and surface operation environments. (2) Verify environmental testing and analysis have adequately validated the flight hardware's ability to withstand all natural, self-induced, and mission-activity-induced environments. The planned tests to ascertain the capability of the MSL to perform as desired are reviewed.

spacecraft↗

Biomimetics as a Model for Inspiring Human Innovation

Electroactive polymers (EAP) are human made actuators that are the closest to mimic biological muscles. Technology was advanced to the level that biologically inspired robots are taking increasing roles in the world around us and making science fiction ideas a closer engineering reality. Artificial technologies (AI, AM, and others) are increasingly becoming practical tools for making biologically inspired devices and instruments with enormous potential for space applications. Polymer materials are used to produce figures that resemble human and animals. These materials are widely employed by the movie industry for making acting figures and by the orthopedic industry to construct cyborg components. There are still many challenges ahead that are critical to making such possibilities practical. The annual armwrestling contest is providing an exciting measure of how well advances in EAP are implemented to address the field challenges. There is a need to document natures inventions in an engineering form to possibly inspire new capabilities.

Electroactive Polymers (EAP)↗

Evaluating the feasibility of biological waste processing for long term space missions

Recycling waste products during orbital (e.g., International Space Station) and planetary missions (e.g., lunar base, Mars transit mission, Martian base) will reduce storage and resupply costs. Wastes streams on the space station will include human hygiene water, urine, faeces, and trash. Longer term missions will contain human waste and inedible plant material from plant growth systems used for atmospheric regeneration, food production, and water recycling. The feasibility of biological and physical-chemical waste recycling is being investigated as part of National Aeronautics and Space Administration's (NASA) Advanced Life Support (ALS) Program. In-vessel composting has lower manpower requirements, lower water and volume requirements, and greater potential for sanitization of human waste compared to alternative bioreactor designs such as continuously stirred tank reactors (CSTR). Residual solids from the process (i.e. compost) could be used a biological air filter, a plant nutrient source, and a carbon sink. Potential in-vessel composting designs for both near- and long-term space missions are presented and discussed with respect to the unique aspects of space-based systems.

NASA Discipline Life Support Systems↗

Prebiotic Chemistry of Pluto

We present the case for the presence of complex organic molecules, such as amino acids and nucleobases, formed by abiotic processes on the surface and in near-subsurface regions of Pluto. Pluto's surface is tinted with a range of non-ice substances with colors ranging from light yellow to red to dark brown; the colors match those of laboratory organic residues called tholins. Tholins are broadly characterized as complex, macromolecular organic solids consisting of a network of aromatic structures connected by aliphatic bridging units (e.g., Imanaka et al.,2004; Materese et al.,2014, 2015). The synthesis of tholins in planetary atmospheres and in surface ices has been explored in numerous laboratory experiments, and both gas- and solid-phase varieties are found on Pluto. A third variety of tholins, exposed at a site of tectonic surface fracturing called Virgil Fossae, appears to have come from a reservoir in the subsurface. Eruptions of tholin-laden liquid H2O from a subsurface aqueous repository appear to have covered portions of Virgil Fossae and its surroundings with a uniquely colored deposit (D.P. Cruikshank, personal communication) that is geographically correlated with an exposure of H2O ice that includes spectroscopically detected NH3 (C.M. Dalle Ore, personal communication). The subsurface organic material could have been derived from presolar or solar nebula processes, or might have formed in situ. Photolysis and radiolysis of a mixture of ices relevant to Pluto's surface composition (N2, CH4, CO) have produced strongly colored, complex organics with a significant aromatic content having a high degree of nitrogen substitution similar to the aromatic heterocycles pyrimidine and purine (Materese et al.,2014, 2015; Cruikshank et al.,2016). Experiments with pyrimidines and purines frozen in H2O-NH3 ice resulted in the formation of numerous nucleobases, including the biologically relevant guanine, cytosine, adenine, uracil, and thymine (Materese et al.,2017). The red material associated with the H2O ice may contain nucleobases resulting from energetic processing on Pluto's surface or in the interior. Some other Kuiper Belt objects also exhibit red colors similar to those found on Pluto and may therefore carry similar inventories of complex organic materials. The widespread and ubiquitous nature of similarly complex organic materials observed in a variety of astronomical settings drives the need for additional laboratory and modeling efforts to explain the origin and evolution of organic molecules. Pluto observations reveal complex organics on a small body that remains close to its place of origin in the outermost regions of the Solar System.

Cruikshank, D. P.↗

Low Gravity Materials Science Research for Space Exploration

On January 14, 2004, the President of the United States announced a new vision for the United States civil space program. The Administrator of the National Aeronautics and Space Administration (NASA) has the responsibility to implement this new vision. The President also created a Presidential Commission 'to obtain recommendations concerning implementation of the new vision for space exploration.' The President's Commission recognized that achieving the exploration objectives would require significant technical innovation, research, and development in focal areas defined as 'enabling technologies.' Among the 17 enabling technologies identified for initial focus were advanced structures; advanced power and propulsion; closed-loop life support and habitability; extravehicular activity system; autonomous systems and robotics; scientific data collection and analysis; biomedical risk mitigation; and planetary in situ resource utilization. The Commission also recommended realignment of NASA Headquarters organizations to support the vision for space exploration. NASA has aggressively responded in its planning to support the vision for space exploration and with the current considerations of the findings and recommendations from the Presidential Commission. This presentation will examine the transformation and realignment activities to support the vision for space exploration that are underway in the microgravity materials science program. The heritage of the microgravity materials science program, in the context of residence within the organizational structure of the Office of Biological and Physical Research, and thematic and sub-discipline based research content areas, will be briefly examined as the starting point for the ongoing transformation. Overviews of future research directions will be presented and the status of organizational restructuring at NASA Headquarters, with respect to influences on the microgravity materials science program, will be discussed. Additional information is included in the original extended abstract.

Clinton, R. G., Jr.↗

Collecting Ground Samples for Balloon-Borne Instruments

A proposed system in a gondola containing scientific instruments suspended by a balloon over the surface of the Saturn moon Titan would quickly acquire samples of rock or ice from the ground below. Prototypes of a sample-collecting device that would be a major part of the system have been tested under cryogenic and non-cryogenic conditions on Earth. Systems like this one could also be used in non-cryogenic environments on Earth to collect samples of rock, soil, ice, mud, or other ground material from such inaccessible or hazardous locations as sites of suspected chemical spills or biological contamination. The sample-collecting device would be a harpoonlike device that would be connected to the balloon-borne gondola by a tether long enough to reach the ground. The device would be dropped from the gondola to acquire a sample, then would be reeled back up to the gondola, where the sample would be analyzed by the onboard instruments. Each prototype of the sample-collecting device has a sharp front (lower) end, a hollow core for retaining a sample, a spring for holding the sample in the hollow core, and a rear (upper) annular cavity for retaining liquid sample material. Aerodynamic fins at the rear help to keep the front end pointed downward. In tests, these prototype devices were dropped from various heights and used to gather samples of dry sand, moist sand, cryogenic water ice, and warmer water ice.

Jones, Jack↗