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NASA NTRS1994

A Macintosh-Based Scientific Images Video Analysis System

A set of experiments was designed at MIT's Man-Vehicle Laboratory in order to evaluate the effects of zero gravity on the human orientation system. During many of these experiments, the movements of the eyes are recorded on high quality video cassettes. The images must be analyzed off-line to calculate the position of the eyes at every moment in time. To this aim, I have implemented a simple inexpensive computerized system which measures the angle of rotation of the eye from digitized video images. The system is implemented on a desktop Macintosh computer, processes one play-back frame per second and exhibits adequate levels of accuracy and precision. The system uses LabVIEW, a digital output board, and a video input board to control a VCR, digitize video images, analyze them, and provide a user friendly interface for the various phases of the process. The system uses the Concept Vi LabVIEW library (Graftek's Image, Meudon la Foret, France) for image grabbing and displaying as well as translation to and from LabVIEW arrays. Graftek's software layer drives an Image Grabber board from Neotech (Eastleigh, United Kingdom). A Colour Adapter box from Neotech provides adequate video signal synchronization. The system also requires a LabVIEW driven digital output board (MacADIOS II from GW Instruments, Cambridge, MA) controlling a slightly modified VCR remote control used mainly to advance the video tape frame by frame.

Groleau, Nicolas↗
NASA NTRS2006

Modification of Eye Movements and Motion Perception during Off-Vertical Axis Rotation

Constant velocity Off-Vertical Axis Rotation (OVAR) imposes a continuously varying orientation of the head and body relative to gravity. The ensuing ocular reflexes include modulation of both torsional and horizontal eye movements as a function of the varying linear acceleration along the lateral plane, and modulation of vertical and vergence eye movements as a function of the varying linear acceleration along the sagittal plane. Previous studies have demonstrated that tilt and translation otolith-ocular responses, as well as motion perception, vary as a function of stimulus frequency during OVAR. The purpose of this study is to examine normative OVAR responses in healthy human subjects, and examine adaptive changes in astronauts following short duration space flight at low (0.125 Hz) and high (0.5 Hz) frequencies. Data was obtained on 24 normative subjects (14 M, 10 F) and 14 (13 M, 1F) astronaut subjects. To date, astronauts have participated in 3 preflight sessions (n=14) and on R+0/1 (n=7), R+2 (n= 13) and R+4 (n= 13) days after landing. Subjects were rotated in darkness about their longitudinal axis 20 deg off-vertical at constant rates of 45 and 180 deg/s, corresponding to 0.125 and 0.5 Hz. Binocular responses were obtained with video-oculography. Perceived motion was evaluated using verbal reports and a two-axis joystick (pitch and roll tilt) mounted on top of a two-axis linear stage (anterior-posterior and medial-lateral translation). Eye responses were obtained in ten of the normative subjects with the head and trunk aligned, and then with the head turned relative to the trunk 40 deg to the right or left of center. Sinusoidal curve fits were used to derive amplitude, phase and bias of the responses over several cycles at each stimulus frequency. Eye responses during 0.125 Hz OVAR were dominated by modulation of torsional and vertical eye position, compensatory for tilt relative to gravity. While there is a bias horizontal slow phase velocity (SPV), the modulation of horizontal and vergence SPV is negligible at this lower stimulus frequency. Eye responses during 0.5 Hz OVAR; however, are characterized by modulation of horizontal and vergence SPV, compensatory for translation in the lateral and sagittal planes, respectively. Neither amplitude nor bias velocities were significantly altered by head-on-trunk position. The phases of the ocular reflexes, on the other hand, shifted towards alignment with the head. During the lower frequency OVAR, subjects reported the perception of progressing along the edge of a cone. During higher frequency OVAR, subjects reported the perception of progressing along the edge of an upright cylinder. In contrast to the eye movements, the phase of both perceived tilt and translation motion is not altered by stimulus frequency. Preliminary results from astronaut data suggest that the ocular responses are not substantially altered by short-duration spaceflight. However, compared to preflight averages, astronauts reported greater amplitude of both perceived tilt and translation at low and high frequency, respectively, during early post-flight testing. We conclude that the neural processing to distinguish tilt and translation linear acceleration stimuli differs between eye movements and motion perception. The results from modifying head-on-trunk position are consistent with the modulation of ocular reflexes during OVAR being primarily mediated by the otoliths in response to the sinusoidally varying linear acceleration along the interaural and naso-occipital head axis. While the tilt and translation ocular reflexes appear to operate in an independent fashion, the timing of perceived tilt and translation influence each other. We conclude that the perceived motion path during linear acceleration in darkness results from a composite representation of tilt and translation inputs from both vestibular and somatosensory systems.

Wood, S. J.↗
NASA NTRS2001

Effect of Marangoni Convection Generated by Voids on Segregation During Low-G and 1-G Solidification

Solidification experiments, especially microgravity solidification experiments, are often compromised by the evolution of unwanted voids or bubbles in the melt. Although these voids and/or bubbles are highly undesirable, there is currently no effective means of preventing their formation or of eliminating their adverse effects, particularly during microgravity experiments. Marangoni convection caused by these voids can drastically change the transport processes in the melt. Recent microgravity experiments by Matthiesen (1) Andrews (2) and Fripp (3) are perfect examples of how voids and bubbles can affect the outcome of costly space experiments and significantly increase the level of difficulty in interpreting their results. Formation of bubbles have caused problems in microgravity experiments for a long time. Even in the early Skylab mission an unexpectedly large number of bubbles were detected in the four materials processing experiments reported by Papazian and Wilcox (4). They demonstrated that while during ground-based tests bubbles were seen to detach from the interface easily and float to the top of the melt, in low-gravity tests no detachment from the interface occurred and large voids were grown in the crystal. More recently, the lead-tin-telluride crystal growth experiment of Fripp et al.(3) flown aboard the USMP-3 mission has provided very interesting results. The purpose of the study was to investigate the effect of natural convection on the solidification process by growing the samples at different orientations with respect to the gravitational field. Large pores and voids were found in the three solid crystal samples processed in space. Post-growth characterization of the compositional profiles of the cells indicated considerable levels of mixing even in the sample grown in the hot-on-top stable configuration. The mixing was attributed to thermocapillary convection caused by the voids and bubbles which evolved during growth. Since the thermocapillary convection is orientation-independent, diffusion-controlled growth was not possible in any of the samples, even the top-heated one. These results are consistent with recent studies of thermocapillary convection generated by a bubble on a heated surface undertaken by Kassemi and Rashidnia (5-7) where it is numerically and experimentally shown that the thermocapillary flow generated by a bubble in a model fluid (silicone oil) can drastically modify the temperature field through vigorous mixing of the fluid around it, especially under microgravity conditions.

Kassemi, M.↗
NASA NTRS2002

Investigation of Body Force Effects on Flow Boiling Critical Heat Flux

The bubble coalescence and interfacial instabilities that are important to modeling critical heat flux (CHF) in reduced-gravity systems can be sensitive to even minute body forces. Understanding these complex phenomena is vital to the design and safe implementation of two-phase thermal management loops proposed for space and planetary-based thermal systems. While reduced gravity conditions cannot be accurately simulated in 1g ground-based experiments, such experiments can help isolate the effects of the various forces (body force, surface tension force and inertia) which influence flow boiling CHF. In this project, the effects of the component of body force perpendicular to a heated wall were examined by conducting 1g flow boiling experiments at different orientations. FC-72 liquid was boiled along one wall of a transparent rectangular flow channel that permitted photographic study of the vapor-liquid interface at conditions approaching CHF. High-speed video imaging was employed to capture dominant CHF mechanisms. Six different CHF regimes were identified: Wavy Vapor Layer, Pool Boiling, Stratification, Vapor Counterflow, Vapor Stagnation, and Separated Concurrent Vapor Flow. CHF showed great sensitivity to orientation for flow velocities below 0.2 m/s, where very small CHF values where measured, especially with downflow and downward-facing heated wall orientations. High flow velocities dampened the effects of orientation considerably. Figure I shows representative images for the different CHF regimes. The Wavy Vapor Layer regime was dominant for all high velocities and most orientations, while all other regimes were encountered at low velocities, in the downflow and/or downward-facing heated wall orientations. The Interfacial Lift-off model was modified to predict the effects of orientation on CHF for the dominant Wavy Vapor Layer regime. The photographic study captured a fairly continuous wavy vapor layer travelling along the heated wall while permitting liquid contact only in wetting fronts, located in the troughs of the interfacial waves. CHF commenced when wetting fronts near the outlet were lifted off the wall. The Interfacial Lift-off model is shown to be an effective tool for predicting the effects of body force on CHF at high velocities.

Zhang, Hui↗
NASA NTRS2001

Expression of the Acyl-Coenzyme A: Cholesterol Acyltransferase GFP Fusion Protein in Sf21 Insect Cells

The enzyme acyl-coenzyme A:cholesterol acyltransferase (ACAT) is an important contributor to the pathological expression of plaque leading to artherosclerosis n a major health problem. Adequate knowledge of the structure of this protein will enable pharmaceutical companies to design drugs specific to the enzyme. ACAT is a membrane protein located in the endoplasmic reticulum.t The protein has never been purified to homogeneity.T.Y. Chang's laboratory at Dartmouth College provided a 4-kb cDNA clone (K1) coding for a structural gene of the protein. We have modified the gene sequence and inserted the cDNA into the BioGreen His Baculovirus transfer vector. This was successfully expressed in Sf2l insect cells as a GFP-labeled ACAT protein. The advantage to this ACAT-GFP fusion protein (abbreviated GCAT) is that one can easily monitor its expression as a function of GFP excitation at 395 nm and emission at 509 nm. Moreover, the fusion protein GCAT can be detected on Western blots with the use of commercially available GFP antibodies. Antibodies against ACAT are not readily available. The presence of the 6xHis tag in the transfer vector facilitates purification of the recombinant protein since 6xHis fusion proteins bind with high affinity to Ni-NTA agarose. Obtaining highly pure protein in large quantities is essential for subsequent crystallization. The purified GCAT fusion protein can readily be cleaved into distinct GFP and ACAT proteins in the presence of thrombin. Thrombin digests the 6xHis tag linking the two protein sequences. Preliminary experiments have indicated that both GCAT and ACAT are expressed as functional proteins. The ultimate aim is to obtain large quantities of the ACAT protein in pure and functional form appropriate for protein crystal growth. Determining protein structure is the key to the design and development of effective drugs. X-ray analysis requires large homogeneous crystals that are difficult to obtain in the gravity environment of earth. Protein crystals grown in microgravity are often larger and have fewer defects than those grown on earth. The analysis of higher quality space-grown crystals will assist in structure-based drug design. We have successfully grown GCAT-infected Sf21 cells in both adhesion and suspension cultures. Expression levels of GCAT in cell lines such as Sf9 and High Five appear to be reduced. We intend to replicate GCAT expression in all three cell lines using the NASA rotating wall bioreactor which effectively duplicates a microgravity environment. The bioreactor itself could be launched to study the expression of the GFP and GCAT proteins in the actual microgravity environment achieved in orbit.

Mahtani, H. K.↗
NASA NTRS2013

Microgravity Drill and Anchor System

This work is a method to drill into a rock surface regardless of the gravitational field or orientation. The required weight-on-bit (WOB) is supplied by a self-contained anchoring mechanism. The system includes a rotary percussive coring drill, forming a complete sampling instrument usable by robot or human. This method of in situ sample acquisition using micro - spine anchoring technology enables several NASA mission concepts not currently possible with existing technology, including sampling from consolidated rock on asteroids, providing a bolt network for astronauts visiting a near-Earth asteroid, and sampling from the ceilings or vertical walls of lava tubes and cliff faces on Mars. One of the most fundamental parameters of drilling is the WOB; essentially, the load applied to the bit that allows it to cut, creating a reaction force normal to the surface. In every drilling application, there is a minimum WOB that must be maintained for the system to function properly. In microgravity (asteroids and comets), even a small WOB could not be supported conventionally by the weight of the robot or astronaut. An anchoring mechanism would be needed to resist the reactions, or the robot or astronaut would push themselves off the surface and into space. The ability of the system to anchor itself to a surface creates potential applications that reach beyond use in low gravity. The use of these anchoring mechanisms as end effectors on climbing robots has the potential of vastly expanding the scope of what is considered accessible terrain. Further, because the drill is supported by its own anchor rather than by a robotic arm, the workspace is not constrained by the reach of such an arm. Yet, if the drill is on a robotic arm, it has the benefit of not reflecting the forces of drilling back to the arm s joints. Combining the drill with the anchoring feet will create a highly mobile, highly stable, and highly reliable system. The drilling system s anchor uses hundreds of microspine toes that independently find holes and ledges on a rock to create an anchor. Once the system is anchored, a linear translation mechanism moves the drill axially into the surface while maintaining the proper WOB. The linear translation mechanism is composed of a ball screw and stepper motor that can translate a carriage with high precision and applied load. The carriage slides along rails using self-aligning linear bearings that correct any axial misalignment caused by bending and torsion. The carriage then compresses a series of springs that simultaneously transmit the load to the drill along the bit axis and act as a suspension that compensates for the vibration caused by percussive drilling. The drill is a compacted, modified version of an off-the-shelf rotary percussive drill, which uses a custom carbide-tipped coring bit. By using rotary percussive drilling, the drill time is greatly reduced. The percussive action fractures the rock debris, which is removed during rotation. The final result is a 0.75-in. (.1.9- cm) diameter hole and a preserved 0.5- in. (.1.3-cm) diameter rock core. This work extends microspine technology, making it applicable to astronaut missions to asteroids and a host of robotic sampling concepts. At the time of this reporting, it is the first instrument to be demonstrated using microspine anchors, and is the first self-contained drill/anchor system to be demonstrated that is capable of drilling in inverted configurations and would be capable of drilling in microgravity.

Parness, Aaron↗
NASA NTRS2011

Reducing NPR 7120.5D to Practice: Transitioning from Design Reviews to the SIR Hardware Review

The Gravity Recovery And Interior Laboratory (GRAIL) mission was the first Jet Propulsion Laboratory (JPL) project initiated under NASA's revised rules for space flight project management, NPR 7120.5D, "NASA Space Flight Program and Project Management Requirements." NASA selected GRAIL through a competitive Announcement of Opportunity process and funded its Phase B Preliminary Design effort. The team's first major milestone was a JPL institutional milestone, the Project Mission System Review (PMSR), which proved an excellent tune-up for the end-of-Phase-B NASA life-cycle review, the Preliminary Design Review (PDR). Building on JPL experience on the Prometheus and Juno projects, the team successfully organized for and conducted these reviews on an aggressive schedule. For the Project Critical Design Review (CDR), lessons learned from the PDR and updated Standing Review Board (SRB) practices from the Agency were factored into the review preparation effort. Additionally, the review was held at the Principal Investigator's institution, the Massachusetts Institute of Technology, rather than at the project management center (JPL), which necessitated additional cross-country coordination steps. The PMSR, PDR, and CDR were design reviews and largely paper-oriented. For the System Integration Review (SIR), the project needed to transition to a hardware review and deal with paper in a very different manner. While many of the practices employed for the design reviews were modified and retained (e.g., review preparation team, gate products management, pre-reviews, SRB coordination), the review agenda, presentation style, and slide templates were significantly changed. A key success factor concerned the handling of project open paper, which was succinctly and effectively communicated to the SRB in presentations.This paper provides a brief overview of the GRAIL mission and its project management challenges, provides a detailed description of project SIR preparation and execution activities, including positive and negative lessons learned and identifies recommendations for future NASA (and non- NASA) project teams.

NPR 7120.5D↗
NASA NTRS2018

Rapid Model Import Tool (RMIT)

Our project is about developing a tool to implement conversion of 3D Computer Aided Design (CAD) models produced with software such as Delmia, 3DS Max, or Maya, into a size and format compatible with the Unity 3D environment. RMIT will be used to aid KSC engineering personnel in the design, development, testing, operations, and training on spacecraft, launch vehicles, facilities, and ground support equipment. For our project, we are using Blender, a free/open-source 3D graphics software, with the goal of developing, testing, and deploying a 3D CAD model converter tool. I worked on using Blender to import 3D CAD models exported from CATIA software into Collada file format. The Collada file format has file extension DAE. Importing the Collada DAE file as is into Blender, generates dots and dashes. In 3DS Max, there is an existing OpenCollada plugin and with that plugin, 3DS Max can import the DAE file successfully. But, Blender does not seem to have an OpenCollada plugin, so I worked on writing a new OpenCollada plugin for Blender. Since 3DS Max was able to display the image, I looked into comparing differences between the original DAE file and the DAE file exported from 3DS Max using the OpenCollada plugin. As Collada documents describing digital assets are XML files with file extension DAE, Collada files contains XML tags, making them easily modifiable. After some research, it appears that Blender does not like primitive 2D tags like tristrips and trifans. Changing those tags to polygons slightly improved the image, but the pieces were exploded. I found after further research comparing differences between the original file and the file exported from 3DS Max that the values inside the translate tags in the original file are scaled down by a factor of 25.4 in the exported file from 3DS Max, representing the millimeters to inches conversion (1 inch = 25.4 millimeters). After scaling down values inside all of the translate tags by 25.4, the exploded pieces stuck back in, but the image needed further improvement. I have been able to create a new plugin in Blender that takes the original DAE file, replaces the primitive 2D tags tristrips and trifans with polygons, scales down the values inside the translate tags by a factor of 25.4, and saves the changes into a temporary DAE file. After the temporary DAE file is imported into Blender, the temp file is then deleted, keeping the original DAE file intact. Starting with a DAE file that is exported using the NASA Enterprise Visualization Application (NEVA), a Collada exporter, from CATIA gives better results. NEVA is a Design Visualization product that is used for exporting 3D models from CATIA. With NEVA, the up axis is defined in the top-level node if navigation gravity is enabled. With that file, just replacing the primitive tags tristrips and trifans with polygons in yields a much improved image in Blender. As we identify more differences between the original DAE file and the DAE file exported from 3DS Max, this plugin can be improved further. Our goal is to have a model that is formatted and sized for import into Unity, and we are trying out different 3D programs to see which will work best.

Ayyangar, Arjun↗
NASA NTRSDate not supplied

A Heritage BioSensor for Lunar Biology Experiments

Introduction: Automated biological experiments on small spacecraft missions have gained prominence over the past decade due to their simplicity, accessibility, and small mass, volume, and power needs. Most recently, the BioSensor microfluidic CubeSat payload aboard BioSentinel used an automated microfluidic cell culture system to study the effects of environmental stressors like deep space radiation and microgravity on yeast growth and metabolism. BioSentinel’s successor, the Lunar Explorer Instrument for space biology Applications (LEIA), will study the effects of lunar gravity and radiation using an improved version of the BioSensor microfluidic platform. The BioSensor payload has great adaptability to host a diverse range of biological experiments with single- and multi-celled organisms in both crewed and uncrewed missions, making it a compelling candidate for future space biology studies in a lunar surface environment. BioSensor Instrumentation on BioSentinel: The first spaceflight mission with the BioSensor, BioSentinel’s biology experiments occurred at three locations -- deep space, ISS and ground. The payload contained 18 microfluidic cards, each featuring 16 growth wells (a total of 288 growth wells). Each well was loaded before launch with desiccated yeast. In space, liquid culture medium (nutrients) was automatically introduced to batches of wells at a time to initiate a series of biology experiments. Temperature was maintained by thin film heaters on both sides of each card. Each well was equipped with three LEDs emitting at 570 nm, 630 nm, and 850 nm, paired with photodetectors to measure cell concentration and the alamarBlue (metabolic indicator dye) color transition from blue to pink. Phenotypic parameters like cell viability, metabolic rate, and generation time can be derived from these measurements. The sequence and timing of fluid fills, optical measurements, and thermal control were stored onboard, but could be updated asynchronously via ground communication. LEIA: LEIA is slated for launch no earlier than 2026 on a CLPS lander. BioSentinel’s BioSensor has been modified for use in LEIA. These improvements include: (a) storage for multiple culture medium types, (b) additional LED color (465 nm) for a new biological assay for antioxidant (carotenoid) production, (c) housing modifications for later biology load before launch, (d) improved isolation between electronic and fluidic components, and (e) improved humidity control for prolonged organism viability in case of post-load launch delay. Future Prospects: The consistent and successful demonstration of complex fluidics platforms alongside reliable instrument operations in a space environment is poised to create strong momentum for BioSensor-based biological experiment payloads. Planned future developments with the BioSensor include extending compatibility to a broader range of organisms and assays. Preliminary work has already demonstrated successful growth of Arabidopsis seedlings in fluidic cards. With a few modifications to the optical assembly, the setup could easily measure photosynthetic traits in plants and cyanobacteria. The addition of fluorescence measurements and generation of novel luminescent assays will elevate BioSensor’s functionality further. Beyond the BioSensor’s potential uses on free-flyer missions, ISS and Gateway, and CLPS landers, deploying the BioSensor to the lunar surface or in an artificial habitat on crewed missions could enable pioneering research on both how life responds to lunar conditions and future bioproduction capabilities making the BioSensor an indispensable tool for future space biology research.

Chinmayee Govinda Raj↗
NASA NTRSDate not supplied

Options for Offloading a 90-Ton Common Habitat from its Lander on the Surface of Mars

The Common Habitat is a large, long-duration habitat being explored as part of a conceptual study (not an active NASA program) that uses an SLS core stage liquid oxygen (LOX) tank as its primary structure. Measuring 8.4 meters in diameter and 15.6 meters in length, it is manufactured as a habitat and launched as such into space. It is intended for use on the Moon as part of a permanently occupied outpost, on Mars as part of an outpost that will be occupied for hundreds of days at a time, and in deep space as part of the Deep Space Exploration Vehicle where it will support crewed missions up to 1200 days in duration. A study of internal orientation and crew size resulted in a Common Habitat configuration sized for a crew of eight with a three-deck horizontal orientation. There are obvious challenges associated with the delivery of such a large habitat, which may mass as much as 90-tons when initially deployed. The Mars destination in particular imposes extreme challenges due to Martian gravity. This paper identifies initial options for the offloading of a 90-ton Common Habitat from a lander spacecraft on the surface of Mars. On Mars, the Common Habitat is part of a surface outpost where a Habitation Zone includes the Common Habitat docked to a two-chamber airlock node, up to two logistics modules, and up to two pressurized rovers. It is connected by underground conduit to a radiator farm and communications tower assembly. These elements and other surface infrastructure, including robotic systems for surface preparation, are landed prior to the Common Habitat. In the baseline Common Habitat Architecture, the Common Habitat is delivered on the third heavy cargo flight. The Habitation Zone configuration dictates that the Common Habitat needs to be offloaded from the lander. All of the docked elements require direct access to the surface and the Common Habitat must actually be placed in a trench to lower its docking ports to be level with those of the mated elements. Additionally, the habitat must be emplaced in a horizontal configuration, while for any conceivable Earth launch system it must be launched in a vertical configuration. It is true that the Common Habitat must be offloaded from its lander on both the Moon and Mars and a common offloading system must therefore work in both destinations. Mars, however, is considered the driving case for offloading in most, but not all, aspects. A four-day internal study in 2021 recommended that a modified Starship be used to land the Common Habitat on Mars and considered multiple approaches to offload the Common Habitat from the payload section and lower it to the surface. The topic was presented at a public hackathon organized by the Johnson Space Center’s Emerge Employee Resource Group. One team took on the challenge and proposed a concept in some ways similar to the previously considered jib crane. Despite the excellent innovation in the team’s work, a number of study refinements are necessary to truly establish feasibility. These and other future work needed to mature the concept are discussed in this work.

Lander Offloading↗
NASA NTRSDate not supplied

Next Generation Exercise Device (NGED): Advancing Exercise Capabilities for Future Space Missions Through Biomechanical Modeling

BACKGROUND As space exploration extends to long-duration missions on the Moon and Mars, maintaining astronaut health and fitness becomes increasingly critical. The Next Generation Exercise Device (NGED), developed and tested by the HumanWorks Lab in NASA Johnson Space Center's (JSC) Software, Robotics, and Simulation Division, aims to address this challenge through innovative approaches. This study presents the development and evaluation of an NGED system, focusing on its adaptability to various mission scenarios, including prospective use in a Lunar Pressurized Rover (LPR). Central to this project is the application of biomechanical modeling to optimize exercise efficacy and safety in microgravity and partial gravity environments. The project is a collaborative effort with the Human Health and Performance group at Johnson Space Center, ensuring a comprehensive approach to astronaut well-being that integrates biomechanical principles with practical exercise solutions. The NGED represents the next generation of exercise capabilities for missions in space, on the Moon and Mars, with a specific focus on applications such as the LPR. METHODS AND RESULTS Data collection for NGED development was conducted with two motor-driven Beyond Power Voltra I [1] systems and a custom test structure to allow placement of the cable-based devices on the ground, at shoulder height, and overhead. The collection was performed in JSC’s Prototype Immersive Technology (PIT) Lab, utilizing an OptiTrack motion capture system and AMTI force platform, to enable detailed biomechanical analysis via OpenSim [2,3]. Motion capture data were collected for three subjects representing different body types and statures. The marker set used was an enhanced version of the full-body Plug-in Gait marker set [4], with additional markers strategically placed for the primary objective of informing exercise volume requirements. Subjects performed a series of 17 exercises, carefully selected to engage various muscle groups, including novel spaceflight exercises such as skiing (ergometer style), lateral pulldowns, wood chops, triceps extensions, and flies, with load variations ranging from 10 to 90 pounds to maintain kinematic form. This comprehensive approach allowed for a thorough evaluation of the NGED's performance across a wide range of motions and loads. The biomechanical modeling and analysis were conducted using a modified OpenSim Full Body Rajagopal Model [4,5] and also scaled to the maximum and minimum anthropometry provided in NASA-STD-3001 [6]. Volumetric convex hulls were generated based on model marker trajectories and aggregated into geometric assemblies. These can be placed in models of vehicle designs to assess fit to protect for exercise as well as to adapt NGED exercise to fit available space. Preliminary findings from the collection indicate that the NGED prototype demonstrates significant adaptability across varying user anthropometrics and exercise types. The device showed consistent performance in load-bearing exercises, with subjects able to perform exercises effectively while maintaining proper biomechanical form. CONCLUSION NGED represents a forward-looking advancement in exercise capabilities for future space missions. In the future, this system can be used to capture valuable metrics (e.g., isometric mid-thigh pull for force output measurements, assessments of postural muscle strength, overall isometric strength). Its versatility in accommodating various exercises and user physiques, coupled with the ability to provide targeted biomechanical loading, makes it a promising approach for maintaining astronaut health during long-duration missions to the Moon and Mars. Future work will focus on refining the NGED based on initial biomechanical findings, leveraging the detailed insights provided by motion capture and analysis techniques. Particular emphasis will be placed on optimizing its use within the confined spaces of a LPR and other space habitats. This work contributes significantly to NASA's goals of supporting human health and performance in deep space exploration, paving the way for sustainable long-term presence beyond Low Earth Orbit through advanced, biomechanically-informed exercise solutions.

C Wang↗
NASA NTRSDate not supplied

Recent Improvements and Verification of A Full Body Model in Opensim

BACKGROUND: The dynamic feasibility [1,2] criterion, that the subject’s Center of Pressure (COP) be located within the Base of Support (BOS) which outlines the feet, has aided in assessing the stability of human motion recorded on earth while performing the recorded tasks in lunar gravity or as countermeasures exercises on a vibration isolation and stabilization system in microgravity. The convex hull of the BOS on the platform under the subject’s feet was estimated using virtual markers on the feet of the scaled subject model. The COP was calculated using the ground reaction forces and moments determined from motion capture data with biomechanical modeling tools [3]. Occasionally, large-amplitude oscillatory spikes or “artifacts” were observed in the subject’s linear and angular momentum derivatives, affecting some COP data derived from motion capture. The purpose of this investigation was to assess and improve the accuracy of model scaling and BOS estimation as well as to determine the efficacy of model adjustments in mitigating artifacts influencing motion capture-derived ground reaction force and COP results. METHODS: To aid evaluation of proposed process and model updates, motion capture data were collected for two subjects during unit test and range of motion trials, lunar tasks, and countermeasures exercise motions. Markers were added to the full body Plug-in Gait marker set [4] during data collection. New markers were placed on the front, back, sides, and top of the head to improve scaling using distances between marker pairs. Medial elbow markers were added to stabilize the upper arm during OpenSim Inverse Kinematics (IK) [5]. Finally, markers were added on the outer edge of the heels and on the outside edges of the first and last toes on each foot. These additional foot markers were made available to test new automated foot scaling techniques and to calculate the error between the subject’s estimated and recorded BOS. The modified unscaled OpenSim Full Body Rajagopal Model [6,7] was adjusted using some previously investigated techniques [8] to mitigate rapid shifts in joint angles occurring during IK, as these were found to cause the spike artifacts observed in subsequent stages of analysis. Since OpenSim models use Euler angles and rotation sequences, the arm axes of rotation were adjusted, and the pelvis order of rotation was changed to minimize the likelihood of encountering “gimbal lock” during common human motion. The model clavicle, arm, elbow, wrist, pelvis, and ankle angle limits were adjusted to better accommodate the full human range of motion seen in exercise and lunar data. The shoulder joint center was calculated using a “pivoting” algorithm [9], and both shoulder joint center and upper arm markers were included during IK to provide additional shoulder stability on a case-by-case basis. The quality of IK results was assessed by three criteria: minimizing error between recorded motion capture markers and model markers, checking for reasonable rates of change in joint angles between fames (i.e., no IK artifacts), and ensuring the absence of spikes in the inertial forces and angular momentum derivatives calculated using a custom OpenSim plugin [10]. RESULTS: The additional markers placed on the subject during data collection allowed the head and feet to be scaled more accurately using distances between new marker pairs. Scaling with BOS markers placed on the subject and removing the limit on subtalar angle resulted in more accurate BOS determination. Unrealistically large changes in joint angles between frames could be reduced by including clavicle, sternum, and medial elbow markers during IK. In cases with large arm ranges of motion, results could be further improved by running IK using medial elbow and virtual shoulder joint center markers. Model adjustments significantly improved the IK results affecting COP calculation and increased the accuracy of BOS estimation.

C A Bell↗
DOE OSTI2026

Rapidity-dependent spin decomposition of the nucleon

We revisit the two-dimensional Fourier transform of generalized parton distributions (GPDs) at nonzero skewness. At 𝜂 = 0 it reduces to the standard impact-parameter density, while at 𝜂 ≠ 0 it is an off-forward amplitude that we interpret as a genuine parton–nucleon correlation. Its overall strength (the transverse-plane integral of the density) is fixed by the GPD at the kinematic point 𝑡 =−𝑐 𝜂 =−4⁢𝜂 2 ⁢𝑚$^{2}_{𝑁}$/(1 − 𝜂 2 ) and decreases monotonically with the rapidity gap Δ⁢𝑦 = ln⁡[(1+𝜂)/(1−𝜂)] = 2 artanh⁡(𝜂). This rapidity dependence implies rapidity-modified Ji identities that connect helicity, orbital, and total angular momenta of the correlation in closed form. To quantify these effects, we construct leading-twist quark and gluon GPDs in a string-based conformal framework: conformal moments are parametrized by linear open- and closed-string Regge trajectories with slopes constrained by parton distribution functions (PDFs), hadron/glueball spectroscopy, and form-factor data, and GPDs are reconstructed over the full (𝑥,𝜂,𝑡) domain by Mellin-Barnes inversion with next-to-leading order evolution. We find qualitative agreement (and fair quantitative agreement within quoted uncertainties) for several moments and selected nonsinglet 𝑥-space channels at 𝜇 = 2 GeV when compared with lattice QCD, while we also identify channels with visible tension and discuss likely sources (PDF priors and 𝑡-slope systematics).

Gauge-gravity dualities↗
DOE OSTI2024

A modified cosmic brane proposal for holographic Renyi entropy

We propose a new formula for computing holographic Renyi entropies in the presence of multiple extremal surfaces. Our proposal is based on computing the wave function in the basis of fixed-area states and assuming a diagonal approximation for the Renyi entropy. For Renyi index n ≥ 1, our proposal agrees with the existing cosmic brane proposal for holographic Renyi entropy. For n < 1, however, our proposal predicts a new phase with leading order (in Newton’s constant G) corrections to the cosmic brane proposal, even far from entanglement phase transitions and when bulk quantum corrections are unimportant. Recast in terms of optimization over fixed-area states, the difference between the two proposals can be understood to come from the order of optimization: for n < 1, the cosmic brane proposal is a minimax prescription whereas our proposal is a maximin prescription. We demonstrate the presence of such leading order corrections using illustrative examples. In particular, our proposal reproduces existing results in the literature for the PSSY model and high-energy eigenstates, providing a universal explanation for previously found leading order corrections to the n < 1 Renyi entropies.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
NASA NTRS2010

Validation of On-Orbit Methodology for the Assessment of Cardiac Function and Changes in the Circulating Volume Using Ultrasound and Braslet-M Occlusion Cuffs

The objective of this joint U.S. - Russian project was the development and validation of an in-flight methodology to assess a number of cardiac and vascular parameters associated with circulating volume and its manipulation in long-duration space flight. Responses to modified Valsalva and Mueller maneuvers were measured by cardiac and vascular ultrasound (US) before, during, and after temporary volume reduction by means of Braslet-M thigh occlusion cuffs (Russia). Materials and Methods: The study protocol was conducted in 14 sessions on 9 ISS crewmembers, with an average exposure to microgravity of 122 days. Baseline cardiovascular measurements were taken by echocardiography in multiple modes (including tissue Doppler of both ventricles) and femoral and jugular vein imaging on the International Space Station (ISS). The Braslet devices were then applied and measurements were repeated after >10 minutes. The cuffs were then released and the hemodynamic recovery process was monitored. Modified Valsalva and Mueller maneuvers were used throughout the protocol. All US data were acquired by the HDI-5000 ultrasound system aboard the ISS (ATL/Philips, USA) during remotely guided sessions. The study protocol, including the use of Braslet-M for this purpose, was approved by the ISS Human Research Multilateral Review Board (HRMRB). Results: The effects of fluid sequestration on a number of echocardiographic and vascular parameters were readily detectable by in-flight US, as were responses to respiratory maneuvers. The overall volume status assessment methodology appears to be valid and practical, with a decrease in left heart lateral E (tissue Doppler) as one of the most reliable measures. Increase in the femoral vein cross-sectional areas was consistently observed with Braslet application. Other significant differences and trends within the extensive cardiovascular data were also observed. (Decreased - RV and LV preload indices, Cardiac Output, LV E all maneuvers, LV Stroke Volume). Conclusions: This Study: 1) Addressed specific aspects of operational space medicine and space physiology, including assessment of circulating volume disturbances 2) Expanded the applications of diagnostic ultrasound imaging and Doppler techniques in microgravity. 3) Used respiratory maneuvers against the background of acute circulating volume manipulations which appear to enhance our ability to noninvasively detect volume-dependency in a number of cardiac and vascular parameters. 4) Determined that Tei index is not clinically changed therefore contractility not altered in the face of reduced preload. 5) Determined that increased Femoral Vein Area indicating blood being sequestered in lower extremities correlates with reduced preload and cardiac output. 6) That Braslet may be the only feasible means of acutely treating high pressure pulmonary edema in reduced gravity environments.

Hamilton, Douglas↗
NASA NTRS1986

Statolith formation in Cnidaria: effects of cadmium on Aurelia statoliths

Statolith formation in Cnidaria was reviewed with an emphasis on Aurelia statoliths. The review provides information on the chemical composition, mechanisms of initiation of mineralization, and effects of environmental factors on Cnidarian statolith formation. Environmental factors discussed included modified sea water ingredients, X-irradiation, clinostat rotation, and petroleum oil ingredients. A detailed account of the effects of cadmium on mineralization and demineralization of Aurelia statoliths is given. Cadmium at dosages of 2 to 4 micromoles significantly reduces statolith numbers in developing ephyrae. At a dosage of 3 micromoles, cadmium accelerates statolith loss in unfed ephyrae studied at 4 and 8 days following ephyrae release from strobilae. Cadmium, therefore, is shown to reduce statolith numbers in developing ephyrae and to cause greater reduction of statolith numbers in unfed ephyrae after 4 and 8 days than occurred in controls. Supplementation of Cd(2+)-containing artificial sea water (ASW) with calcium (3X and 5X ASW calcium content) results in higher numbers of statoliths at day 4 as compared with cadmium-treated ephyrae. At 8 days only the 5X calcium supplemented ASW is effective in enhancing statolith numbers in Cd(2+)-treated ephyrae. These results suggest that cadmium competes in some manner with calcium at the mineralizing sites of Aurelia.

NASA Discipline Number 40-10↗
NASA NTRS2003

Extracellular ATP inhibits root gravitropism at concentrations that inhibit polar auxin transport

Raising the level of extracellular ATP to mM concentrations similar to those found inside cells can block gravitropism of Arabidopsis roots. When plants are grown in Murashige and Skoog medium supplied with 1 mM ATP, their roots grow horizontally instead of growing straight down. Medium with 2 mM ATP induces root curling, and 3 mM ATP stimulates lateral root growth. When plants are transferred to medium containing exogenous ATP, the gravity response is reduced or in some cases completely blocked by ATP. Equivalent concentrations of ADP or inorganic phosphate have slight but usually statistically insignificant effects, suggesting the specificity of ATP in these responses. The ATP effects may be attributable to the disturbance of auxin distribution in roots by exogenously applied ATP, because extracellular ATP can alter the pattern of auxin-induced gene expression in DR5-beta-glucuronidase transgenic plants and increase the response sensitivity of plant roots to exogenously added auxin. The presence of extracellular ATP also decreases basipetal auxin transport in a dose-dependent fashion in both maize (Zea mays) and Arabidopsis roots and increases the retention of [(3)H]indole-3-acetic acid in root tips of maize. Taken together, these results suggest that the inhibitory effects of extracellular ATP on auxin distribution may happen at the level of auxin export. The potential role of the trans-plasma membrane ATP gradient in auxin export and plant root gravitropism is discussed.

Non-NASA Center↗
NASA NTRS2014

Controllable Multi-Functional Heat Exchanger for Space Applications

Team Spacering will design SpaceRing, a cooling and waste heat recycling system that addresses NASA’s interest for a cooling and power-generating unit in its prototype deep space habitat[1]. The merits of this system are as follows: - Controllable cooling is critical in maintaining a habitable environment and in the functionality of life support systems, powering electronics and other devices in a spacecraft. - Power is expensive to produce in a space environment. Converting wasted thermal energy into electricity will save energy and money for the space station. Space habitation is a highly complex endeavor. Consequently, the SpaceRing must adhere to additional constraints, which include: - Lack of natural fluid or gas convection due to micro-gravity environment - Limited range for heat source and cold sink temperature - Subject to high fluctuations of incoming thermal energy - Minimal weight due to the high cost of transporting items into space After consulting with NASA and experts in thermal studies, several design objectives were identified and are listed below in order of importance: - High cooling capacity - Reliable safety control and alert system - Maximization of energy generation from wasted heat - Optimization of cooling capability and power generation - Long operational life and minimization of maintenance In space, conventional cooling systems utilize passive heat pipe systems for small-scale heat rejection and pumped pipes with heat exchangers for large-scale heat rejection. Thermal power generation in space is mainly achieved by using thermoelectric material that produces electric energy from temperature differentials. We propose a design that combines cooling and thermoelectric power generation. Using a hot pipe connected to a heat source, a cold pipe connected to a radiator, a heat exchanger in contact with both pipes, and a pump in each pipe to move the fluid, we make an active cooling system. The thermoelectric material is inserted in the heat exchanger between the hot and cold pipes to extract energy from the maximum temperature difference coming from the two pipes. It also provides controllable cooling capacity through the use of the thermoelectric material as cooling modules. We have tested our system under target heat source and sink conditions and measured the temperatures and flow rates. Our preliminary results are promising, demonstrating the output of 25 Watts of power on average. We are in the process of running control tests to compare the heat transfer performance without the thermoelectric generators, which is vital in determining whether or not the generators introduce an unacceptable amount of thermal resistance. While some control tests have been run already, the results have been noisy. We have modified our testing environment and data acquisition methods to allow for simple comparison between the control test results and thermoelectric performance results. Lastly, we have modified the heat exchanger design slightly to include acrylic baffles. This will allow the heat to flow through the heat exchanger more efficiently. We have presented our preliminary results to NASA and will deliver the final product by the end of May 2014.

Lee Xiong↗