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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 415 records · Page 23
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

A shift from inorganic to organic nitrogen-dominance shapes soil microbiome composition and co-occurrence networks

Soil microbiomes are characterized by their composition and networks, which are linked to soil nitrogen (N) availability. In nature, inorganic N dominates at one end and organic N dominates at the other end along soil N gradients; however, little is known about how this shift influences soil microbiome composition and co-occurrence networks, as well as their controls. To this end, we conducted an experiment with the host plant Solidago canadensis , which was subject to three N regimes: inorganic N-dominated, co-dominated by inorganic and organic N (CIO), and organic N-dominated. Organic N dominance exhibited stronger effects on the composition and co-occurrence networks of soil microbiomes than inorganic N dominance. The predominant control was plant traits for bacterial and fungal richness, and soil pH for keystone species. Relative to the CIO regime, inorganic N dominance did not affect fungal richness and increased keystone species; organic N dominance decreased fungal richness and keystone species. Pathogenic fungi and arbuscular mycorrhizal fungi were suppressed by organic N dominance but not by inorganic N dominance. These findings suggest that the shift from soil inorganic N-dominance to soil organic N-dominance could strongly shape soil microbiome composition and co-occurrence networks by altering species diversity and topological properties.

Xin, Yue↗
DOE OSTI2024

Severe and mild drought cause distinct phylogenetically linked shifts in the blue grama (Bouteloua gracilis) rhizobiome

Plants rely on a diverse rhizobiome to regulate nutrient acquisition and plant health. With increasing severity and frequency of droughts worldwide due to climate change, untangling the relationships between plants and their rhizobiomes is vital to maintaining agricultural productivity and protecting ecosystem diversity. While some plant physiological responses to drought are generally conserved, patterns of root exudation (release of small metabolites shown to influence microbes) and the consequential effects on the plant rhizobiome can differ widely across plant species under drought. To address this knowledge gap, we conducted a greenhouse study using blue grama ( Bouteloua gracilis ), a drought-tolerant C4 grass native to shortgrass prairie across North American plains, as a model organism to study the effect of increasing drought severity (ambient, mild drought, severe drought) on root exudation and the rhizobiome. Our previous results demonstrated physiological effects of increasing drought severity including an increase in belowground carbon allocation through root exudation and shifts in root exudate composition concurrent with the gradient of drought severity. This work is focused on the rhizobiome community structure using targeted sequencing and found that mild and severe drought resulted in unique shifts in the bacterial + archaeal and fungal communities relative to ambient, non-droughted controls. Specifically, using the change in relative abundance between ambient and drought conditions for each ZOTU as a surrogate for population-scale drought tolerance (e.g., as a response trait), we found that rhizobiome response to drought was non-randomly distributed across the phylogenies of both communities, suggesting that Planctomycetota , Thermoproteota (formerly Thaumarchaeota ), and the Glomeromycota were the primary clades driving these changes. Correlation analyses indicated weak correlations between droughted community composition and a select few root exudate compounds previously implicated in plant drought responses including pyruvic acid, D-glucose, and myoinositol. This study demonstrates the variable impacts of drought severity on the composition of the blue grama rhizobiome and provides a platform for hypothesis generation for targeted functional studies of specific taxa involved in plant-microbe drought responses.

Goemann, Hannah M.↗
DOE OSTI2025

Hepatocellular Metabolic Profile: Understanding Post-Thawing Metabolic Shift in Primary Hepatocytes In Vitro

Primary human hepatocytes (PHHs) are widely used as in vitro models for liver function and drug metabolism studies, yet their metabolic stability post-thawing remains an open question. To better characterize early metabolic changes, we conducted a time-course experiment using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to analyze metabolic shifts in PHHs cultured in suspension. Unexposed and exposed (acetaminophen-treated) samples were evaluated, and TITAN analysis was applied to determine the time point of maximal metabolic change at both individual metabolite and global metabolic profile levels. Our results indicate that the majority of metabolic shifts occur within the first five hours post-thawing. In the early culture time points, substantial metabolic overlap was observed between unexposed and exposed cells, suggesting a conserved biological response likely related to cellular recovery. However, at later time points, metabolite profiles diverged, with acetaminophen treatment-specific metabolic changes emerging, potentially reflecting differences in homeostatic restoration versus hepatotoxic responses. Our study highlights the importance of considering early post-thawing metabolic dynamics in experimental design and offers insights for optimizing hepatocyte culture protocols to better replicate in vivo physiological conditions.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Impact of Shifts in Vegetation Phenology on the Carbon Balance of a Semiarid Sagebrush Ecosystem

Dryland ecosystems are critical in regulating the interannual variability of the global terrestrial carbon cycle. The responses of such ecosystems to weather and environmental conditions remain important factors that limit the accurate projections of carbon balance under future climate change. Here, we investigated how shifts in vegetation phenology resulting from changes in weather and environmental conditions influenced ecosystem carbon cycling in one semiarid ecosystem in the Hanford area of central Washington, United States. We examined two years of measurements of the phenology camera, eddy covariance, and soil chamber from an upland semiarid sagebrush ecosystem. Both years had contrasting diel and seasonal patterns of CO 2 fluxes, primarily driven by differences in vegetation phenology. The net ecosystem exchange of CO 2 (NEE) and evapotranspiration (ET) in 2019 were enlarged by shifted vegetation phenology, as a cold and snow-covered winter and warm and dry winter in 2020 resulted in constrained magnitudes of NEE and ET during the summer months. The annual gross primary productivity (GPP) was much higher in 2019 than in 2020 (-211 vs. -112 gC m -2 ), whereas ecosystem respiration was comparable in these two years (164 vs. 144 gC m -2 ). Thus, the annual NEE in 2019 was negative (-47 gC m -2 ) with the sagebrush ecosystem functioning as a carbon sink, while the positive annual NEE in 2020 indicated that the sagebrush ecosystem functioned as a carbon source. Our results demonstrate that winter snowpack can be a critical driver of annual carbon uptake in semiarid sagebrush ecosystems.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Regime shifts in Arctic terrestrial hydrology manifested from impacts of climate warming

Abstract. Anthropogenic warming in the Arctic is causing hydrological cycle intensification and permafrost thaw, with implications for flows of water, carbon, and energy from terrestrial biomes to coastal zones. To better understand the likely impacts of these changes, we used a hydrology model driven by meteorological data from atmospheric reanalysis and two global climate models for the period 1980–2100. The hydrology model accounts for soil freeze–thaw processes and was applied across the pan-Arctic drainage basin. The simulations point to greater changes over northernmost areas of the basin underlain by permafrost and to the western Arctic. An acceleration of simulated river discharge over the recent past is commensurate with trends drawn from observations and reported in other studies. Between early-century (2000–2019) and late-century (2080–2099) periods, the model simulations indicate an increase in annual total runoff of 17 %–25 %, while the proportion of runoff emanating from subsurface pathways is projected to increase by 13 %–30 %, with the largest changes noted in summer and autumn and across areas with permafrost. Most notably, runoff contributions to river discharge shift to northern parts of the Arctic Basin that contain greater amounts of soil carbon. Each season sees an increase in subsurface runoff; spring is the only season where surface runoff dominates the rise in total runoff, and summer experiences a decline in total runoff despite an increase in the subsurface component. The greater changes that are seen in areas where permafrost exists support the notion that increased soil thaw is shifting hydrological contributions to more subsurface flow. The manifestations of warming, hydrological cycle intensification, and permafrost thaw will impact Arctic terrestrial and coastal environments through altered river flows and the materials they transport.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1972

A simplified, compact static shift register

Shift register was developed which uses only one D type flip-flop and improves packaging density by approximately 25% over the usual arrangement. Circuit is compromise between full master-slave arrangement and dynamic shift register, with limitation only of length of time that clock can be held high during new data entry.

Pryor, R. L.↗
NASA NTRS1972

A method of eliminating hydrogen maser wall shift

Maser output frequency shift was prevented by storage bulb kept at temperature at which wall shift is zero and effects of bulb size, shape, and surface texture are eliminated. Servo system is shown, along with bidirectional counter.

Levine, M. W.↗
NASA NTRS1971

Differential phase shift of partially reflected radio waves.

The addition of phase difference measurements to differential absorption experiments is shown to be both feasible and desirable. The phase information can provide a more sensitive measurement of electron density above about 75 km. The differential phase shift is only weakly dependent on collision frequency in this range, and so an accurate collision frequency profile is not a prerequisite. The differential phase shift and differential absorption measurements taken together can provide both electron density and collision frequency data from about 70 to 90 km.

Connolly, D. J.↗
NASA NTRS1971

Shift and broadening of resonance in turbulent plasmas.

The resonant wave-particle interaction is described, taking into account the effect of the turbulent field on the particle motion in lowest order. It is shown that an electrostatic turbulence produces a shift and a broadening of the Landau resonance. It is found that the resonance shift depends on the mean square spread in wave numbers of the turbulent spectrum, while the resonance width is proportional to the root square of the wave amplitude.

Birmingham, T.↗
NASA NTRS1972

Light and buffer-gas frequency shifts in the Rb-85 maser frequency standard.

The effect of buffer gas and pumping light on the output frequency of the Rb-85 maser is discussed. In contrast to a primary standard, these frequency shifts can be used to advantage when it is necessary to operate the maser at a frequency which differs from the ground-state hyperfine frequency by a few kilohertz. Using appropriate mixtures of buffer gas and carefully shaped lamp spectral profiles, it is also possible to operate the maser exactly at the ground-state hyperfine frequency. It is pointed out that the short-term phase stability of the maser is not impaired by these shifts.

Stern, W. A.↗
NASA NTRS1972

Frequency shifts of ionospheric nfH resonances.

New observational data are analyzed to interpret mechanisms responsible for large positive frequency shifts of Alouette II plasma resonances (corresponding to the first and second electron cyclotron harmonics) relative to frequency values expected from model magnetic field calculations. It is shown that the frequency shifts can be consistently explained by plasma wave dispersion effects combined with sounder transmitter frequency deviation (positive offset of several kilohertz) and a negative offset (several tens of gammas) in the geomagnetic field relative to the model field. Plasma wave dispersion effects are observed on the electron cyclotron second harmonic resonance when it is in the vicinity of the resonance observed near the upper hybrid frequency. The observations suggest that an oblique echo model may be required for interpretation of the electron cyclotron second harmonic resonance.

Benson, R. F.↗
NASA NTRS1972

Speed-dependent collisional width and shift parameters in spectral profiles.

Derivation of an expression for the spectral profile, termed a speed-dependent Voigt profile (SDVP), taking into account speed-dependent shift and width parameters which are calculated on the basis of an active atom-perturber interaction. It is shown that an analysis of line shapes in terms of simple Voigt profiles (rather than the SDVP) may lead to considerable errors in the determination of characteristic atomic parameters, especially in systems where the perturber to active atom mass ratio is large. It is also noted that the speed-dependent shift leads to an asymmetrical profile, and such a profile is displayed.

Berman, P. R.↗
NASA NTRS1973

Four-phase differential phase shift resolver

Two systems have been developed to resolve phase uncertainty without transmitting reference signals. In both methods signal is impressed on carrier as differential, rather than absolute, phase shift. At the receiver four-phase demodulation and logic process unambiguously resolves differential phase shift of input carrier.

Hopkins, P. M.↗
NASA NTRS1974

Doppler shift system

A system is described for measuring velocities of radiating particles based on Doppler shift. Light from the particles is directed through a narrow band optical filter to a Fabry-Perot interferometer initially tuned to a selected center line corresponding to zero particle Doppler shift. The movable mirror of the interferometer is made to sweep about the center line by the output of a modulation oscillator. The fringe pattern output is imaged onto a pin hole through which light is directed to a photomultiplier. The output of the photomultiplier is supplied to a phase sensitive detector with the oscillator output as a reference signal and which operates in the quadrature mode. The detector's output is gain controlled and is combined with the oscillator's output to adjust the interferometer's movable mirror to acquire the line center.

Aisenberg, S.↗
NASA NTRS1974

Differential phase shift keyed signal resolver

A differential phase shift keyed signal resolver resolves the differential phase shift in the incoming signal to determine the data content thereof overcoming phase uncertainty without requiring a transmitted reference signal.

Hopkins, P. M.↗
NASA NTRS1968

Shift register generators and applications to coding

The most important properties of shift register generated sequences are exposed. The application of shift registers as multiplication and division circuits leads to the generation of some error correcting and detecting codes.

Morakis, J. C.↗