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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 415 records · Page 23
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NASA NTRS2002

Expression of the high capacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants

Modulation of cytosolic calcium levels in both plants and animals is achieved by a system of Ca2+-transport and storage pathways that include Ca2+ buffering proteins in the lumen of intracellular compartments. To date, most research has focused on the role of transporters in regulating cytosolic calcium. We used a reverse genetics approach to modulate calcium stores in the lumen of the endoplasmic reticulum. Our goals were two-fold: to use the low affinity, high capacity Ca2+ binding characteristics of the C-domain of calreticulin to selectively increase Ca2+ storage in the endoplasmic reticulum, and to determine if those alterations affected plant physiological responses to stress. The C-domain of calreticulin is a highly acidic region that binds 20-50 moles of Ca2+ per mole of protein and has been shown to be the major site of Ca2+ storage within the endoplasmic reticulum of plant cells. A 377-bp fragment encoding the C-domain and ER retention signal from the maize calreticulin gene was fused to a gene for the green fluorescent protein and expressed in Arabidopsis under the control of a heat shock promoter. Following induction on normal medium, the C-domain transformants showed delayed loss of chlorophyll after transfer to calcium depleted medium when compared to seedlings transformed with green fluorescent protein alone. Total calcium measurements showed a 9-35% increase for induced C-domain transformants compared to controls. The data suggest that ectopic expression of the calreticulin C-domain increases Ca2+ stores, and that this Ca2+ reserve can be used by the plant in times of stress.

NASA Discipline Plant Biology↗
NASA NTRS2004

The Herbig Ae Star HD 163296 in X-Rays

Chandra X-ray imaging spectroscopy of the nearby Herbig Ae star HD 163296 at 100 AU angular resolution is reported. A point-like, soft (kT approximately 0.5 approximately kev), emission-line source is detected at the location of the star with an X-ray luminosity of 4.0e29 erg/s. In addition, faint emission along the direction of a previously-detected Ly-alpha-emitting jet and Herbig-Haro outflow may be present. The relatively low luminosity, lack of a hard spectral component, and absence of strong X-ray variability in HD 163296 can be explained as originating from optically-thin shock-heated gas accreting onto the stellar surface along magnetic field lines. This would require a (dipole) magnetic field strength at the surface of HD 163296 of at least approximately 100 approximately G and perhaps as high as several kG.

Swartz, Douglas A.↗
NASA NTRS2002

Observations of O (VI) Emission from the Diffuse Interstellar Medium Toward l =113 deg b = 71 deg

Emission from 0 VI (lambda lambda 1032, 1038) in the diffuse interstellar medium was observed in two, long Fur Ultra-violet Spectroscopic Explorer (FUSE) observations at l = approximately equals 1l3 degrees .0 and b approximately equals 70 degrees .7 (near the quasar HS 1307+4617). The observed intensities are 2580 +/- 380 (random) +/- 360 (systematic) and 1100 +/- 160 (random) +/- 160 (systematic) photons cm(exp -2)/s s/r in the 1032 and 1038 A emission lines, respectively. The electron density, thermal pressure, and depth of the emitting gas are calculated from the observed intensities. The velocity of the emitting material is about +10 km/s relative to the local standard of rest and less than + 30 km/s relative to the H I along the line of sight. The similarity suggests that the highly ionized gas is quiescent and not recently shock heated. Emission from the C 11 3 s(exp 2) S(sub 1/2) to 2p(exp 2) P(sub 3/2) transition at 1037 A is also observed, and upper limits are placed on the intensities of ultraviolet line emission from C I, C 111, N I, N 11, Mg 11, Si 11, S 11, S 111. S IV, S VI. Fe 11, and Fe 111.

Shelton, R. L.↗
NASA NTRS2003

The Spatial Distribution of Fluorescent H2 Emission Near T Tauri

New subarcsecond far-UV observations of T Tau with Hubble Space Telescope STIS show spatially resolved structure in the 2in. x 2in. area around the star. The structures are apparent in multiline emission of fluorescent H2 pumped by Ly(alpha). One emission structure follows the cavity walls observed around T Tau N in scattered light in the optical. A temperature of greater than or = l000 K is required to have a high enough population in the H2 to produce the observed fluorescent lines; in the cool environment of the T Tau system, shock heating is required to achieve this temperature at distances of a few tens of AU. Fluorescent H2 along the cavity wall represent the best evidence to date for the action of low-density, wide opening angle outflows driving cavities into the molecular medium at scales less than or = 100 AU. A southern region of emission consists of two arcs, with shape and orientation similar to the arcs of H2 2.12 microns and forbidden-line emission crossing the outflow associated with the embedded system T Tau S. This region is located near the centroid of forbidden-line emission at the blueshifted lobe of the north-south outflow.

Saucedo, Jose↗
NASA NTRS2004

Discovery of Extremely Embedded X-ray Sources in the R Coronae Australis Star Forming Core

We detected three extremely embedded X-ray sources in the R Corona Australis (R CrA) star forming core, IRS 7 region. Two weak X-ray sources are associated with the VLA centimeter radio sources 10E & W, whereas the third brightest source detected in the two XMM-Newton observations on March 2003 has no counterpart at any wavelengths. The large K-band upper-limit (19.4m) measured with the University of Hawaii 88-inch Telescope and strong absorption derived in X-rays (N(sub H) approx. 2.8 x 10(exp 23)/sq cm equivalent to A(sub v) approx. 180 m) indicate that the source is younger than typical Class I protostars, i.e. a Class 0 protostar or an intermittent phase between Class 0 and Class I protostars. The X-ray luminosity was less than one thirtieth (log L(sub x) less than or approx. equals 29.3 ergs/s) in the former Chandra observation in October 2000, which suggests that the X-ray activity, probably generated by magnetic activity, is triggered by an intermittent mass accretion episode such as FU Ori type outbursts. Because the source was detected at high significance in the XMM-Newton observations (approx. 2,000 cnts), X-ray properties of such young protostars can be well investigated for the first time. The light curves were constant in the 1st observation and increased linearly by a factor of two during 30 ksec in the 2nd observation. Both spectra showed iron K lines originated in hot thin-thermal plasma and fluorescence by cold gas. They can be reproduced by an absorbed thin-thermal plasma model with a Gaussian component at 6.4 keV (kT approx. 3-4 keV, L(sub x) approx. 7-20 x 10(exp 30) ergs/s). The rising timescale of the light curves in the 2nd observation was too slow for magnetically generated X-ray flares, whereas large equivalent width of the fluorescence iron K line in the 1st observation (approx. 810 eV) requires strong partial covering of the X-ray source. These results suggest that a confined hot (perhaps accretion) spot on the protostellar core was behind the star in the 1st observation and just appeared in the 2nd observation due to the core rotation with period of greater than or approx. 22.8 days, which is much slower than the break-up velocity previously assumed for young protostars. This means that the source had quiescent X-ray activity during the observations with an order of magnitude stronger level than Class I (older) protostars. We also consider whether the X-ray source associated with 10E could be shock heated plasma by a collision of a jet emanating from the tentative Class 0 object.

Hamaguchi, Ken-Ji↗
NASA NTRS1995

Activation of the ATP-ubiquitin-proteasome pathway in skeletal muscle of cachectic rats bearing a hepatoma

Rats implanted with Yoshida ascites hepatoma (YAH) show a rapid and selective loss of muscle protein due mainly to a marked increase (63-95%) in the rate of protein degradation (compared with rates in muscles of pair-fed controls). To define which proteolytic pathways contribute to this increase, epitrochlearis muscles from YAH-bearing and control rats were incubated under conditions that modify different proteolytic systems. Overall proteolysis in either group of rats was not affected by removal of Ca2+ or by blocking the Ca(2+)-dependent proteolytic system. Inhibition of lysosomal function with methylamine reduced proteolysis (-12%) in muscles from YAH-bearing rats, but not in muscles of pair-fed rats. When ATP production was also inhibited, the remaining accelerated proteolysis in muscles of tumor-bearing rats fell to control levels. Muscles of YAH-bearing rats showed increased levels of ubiquitin-conjugated proteins and a 27-kDa proteasome subunit in Western blot analysis. Levels of mRNA encoding components of proteolytic systems were quantitated using Northern hybridization analysis. Although their total RNA content decreased 20-38%, pale muscles of YAH-bearing rats showed increased levels of ubiquitin mRNA (590-880%) and mRNA for multiple subunits of the proteasome (100-215%). Liver, kidney, heart, and brain showed no weight loss and no change in these mRNA species. Muscles of YAH-bearing rats also showed small increases (30-40%) in mRNA for cathepsins B and D, but not for calpain I or heat shock protein 70. Our findings suggest that accelerated muscle proteolysis and muscle wasting in tumor-bearing rats result primarily from activation of the ATP-dependent pathway involving ubiquitin and the proteasome.

NASA Discipline Musculoskeletal↗
NASA NTRS2005

Sulfur Oxidation and Contrail Precursor Chemistry

Sulfuric acid (H2SO4), formed in commercial aircraft operations via Fuel-S (right arrow) SO2 + SO3 (right arrow) H2SO4, plays an important role in affecting the global climate change through atmospheric chemical reactions and radiative forcing. Measurement of the sulfur oxidation rates is critical to the understanding of the contrail formation. The principle reaction pathway is SO2 + O + M (right arrow) SO3 + M. Although there are many measurements for the rates of this reaction, it has never been measured in the temperature and pressure regime available to aircraft operation. In this investigation, a series of experiments were performed behind the reflected shock waves in a shock tube. OH radicals were produced in lean, shock heated SO2/H2/O2/Ar mixtures. The reaction progress was followed using OH absorption spectroscopy at 310 nm. The data were analyzed with the aid of computer modeling/simulation. The mean value of the rate coefficients of R21 determined is k(sub 21,0)/[M]= 3.9 x 10(exp 15) cm(sup 6) per square mole per second at T = 960 - 1150 K and rho = 16-30 micromole per cubic centimeter with uncertainty limits of plus or minus 30%. A non-Arrhenius fit to our data together with all existing data gives k(sub 21,0)/[M] = 1.3 x 10(exp 24) T (exp -2.5) exp(-2350 K/T) cm(sup 6) per square mole per second at T = 300 - 2500 K with the same uncertainty limits given above. The calculated conversion of S(IV)(SO2) to S(VI) (SO3 + H2SO4) was about 2% in our experimental conditions.

DeWitt, Kenneth↗
NASA NTRS2003

Bio-Organic Nanotechnology: Using Proteins and Synthetic Polymers for Nanoscale Devices

While the ability of proteins to self-assemble makes them powerful tools in nanotechnology, in biological systems protein-based structures ultimately depend on the context in which they form. We combine the self-assembling properties of synthetic diblock copolymers and proteins to construct intricately ordered, three-dimensional polymer protein structures with the ultimate goal of forming nano-scale devices. This hybrid approach takes advantage of the capabilities of organic polymer chemistry to build ordered structures and the capabilities of genetic engineering to create proteins that are selective for inorganic or organic substrates. Here, microphase-separated block copolymers coupled with genetically engineered heat shock proteins are used to produce nano-scale patterning that maximizes the potential for both increased structural complexity and integrity.

Molnar, Linda K.↗
NASA NTRS2004

Space Station Biological Research Project (SSBRP) Cell Culture Unit (CCU) and incubator for International Space Station (ISS) cell culture experiments

The CCU and Incubator are habitats under development by SSBRP for gravitational biology research on ISS. They will accommodate multiple specimen types and reside in either Habitat Holding Racks, or the Centrifuge Rotor, which provides selectable gravity levels of up to 2 g. The CCU can support multiple Cell Specimen Chambers, CSCs (18, 9 or 6 CSCs; 3, 10 or 30 mL in volume, respectively). CSCs are temperature controlled from 4-39 degrees C, with heat shock to 45 degrees C. CCU provides automated nutrient supply, magnetic stirring, pH/O2 monitoring, gas supply, specimen lighting, and video microscopy. Sixty sample containers holding up to 2 mL each, stored at 4-39 degrees C, are available for automated cell sampling, subculture, and injection of additives and fixatives. CSCs, sample containers, and fresh/spent media bags are crew-replaceable for long-term experiments. The Incubator provides a 4-45 degrees C controlled environment for life science experiments or storage of experimental reagents. Specimen containers and experiment unique equipment are experimenter-provided. The Specimen Chamber exchanges air with ISS cabin and has 18.8 liters of usable volume that can accommodate six trays and the following instrumentation: five relocatable thermometers, two 60 W power outlets, four analog ports, and one each relative humidity sensor, video port, ethernet port and digital input/output port.

NASA Center ARC↗
NASA NTRS2006

Bombardment History of the Moon: What We Think We Know and What We Don't Know

The absolute impace history of the moon and inner solar system can in principle be derived from the statistics of radiometric ages of shock-heated planetary samples (lunar or meteoritic), from the formation ages of specific impact craters on the moon or Earth; and from agedating samples representing geologic surface units on the moon (or Mars) for which crater densities have been determined. This impact history, however, is still poorly defined. The heavily cratered surface of the moon is a testimony to the importance of impact events in the evolution of terrestrial planets and satellites. Lunar impacts range in scale from an early intense flux of large objects that defined the surface geology of the moon, down to recent, smaller impacts that continually generate and rework the lunar regolith. Densities of larger craters on lunar surface units of dated age define a projectile flux over time that serves as the basis for estimating surface ages on other solid bodies, particularly Mars. The lunar cratering history may address aspects of Earth s evolution, such as the possible role of early intense impacts on the atmosphere and early life and possible periodicity in large impact events in the more recent past. But, much about the lunar impact history remains unknown.. On Earth approximately 172 impact craters up to ~300 km in diameter and up to ~2 Gyr in age are recognized. Although these data suggest greater relative numbers of younger craters, possibly suggesting a recent increase in projectile flux, both the diameters and especially the ages of most terrestrial crates are so poorly known that the differential terrestrial impact flux over time is uncertain. For the moon, densities of craters on some mare surfaces and crater ejecta deposits, for which we have measured or estimated formation ages, suggest an approximately constant lunar impact rate of larger projectiles over the past ~3.5 Gyr. However, the data are cumulative in nature and limited. Questions exist as to how accurately dated samples correlate with surfaces having measured crater densities. Studies of ages of many tiny impact-melt beads from Apollos 12 and 14 soils show a decrease in the number of beads with age from ~4 Gyr ago to ~0.4 Gyr ago, followed by a significant increase in beads with age <0.4 Gyr (2). These authors concluded that the projectile flux had decreased over time, followed by a significant flux increase more recently.

Bogard, Donald↗
NASA NTRS2006

Measurements of Polyatomic Molecule Formation on an Icy Grain Analog Using Fast Atoms

Carbon dioxide has been produced from the impact of a monoenergetic O(P-3) beam upon a surface cooled to 4.8 K and covered with a CO ice. Using temperature-programmed desorption and mass spectrometer detection, we have detected increasing amounts of CO2 formation with O(P-3) energies of 2, 5, 10, and 14 eV. This is the first measurement of polyatomic molecule formation on a surface with superthermal atoms. The goal of this work is to detect other polyatomic species, such as CH3OH, which can be formed under conditions that simulate the grain temperature, surface coverage, and superthermal atoms present in shock-heated circumstellar and interstellar regions.

Chutjian, A.↗
NASA NTRS2006

Ar-Ar Age of NWA-1460 and Evidence For Young Formation Ages of the Shergottites

Agreement of Ar-Ar, Sm-Nd, and Rb-Sr ages for NWA1460, and the inconsistency between a low shock-heating temperature for Zagami and the proposition that a 4.0 Gyr-old Zagami lost most of its Ar-40 are inconsistent with ancient formation ages for these shergottites, but are consistent with relatively young igneous formation ages.

Bogard, Donald D.↗
NASA NTRS2007

The Use of the BAT Instrument on SWIFT for the Detection of Prompt Gamma-Ray Emission from Novae

Gamma-rays are expected to be emitted during and immediately following a nova explosion due to the annihilation of positrons emitted by freshly produced short-lived radioactive isotopes. The expected gammaray emission is relatively short-lived and as nova explosions are unpredictable, the best chance of detecting the gamma-rays is with n wide field instrument. At the time when the flux is expected to rcach its peak, most of the gamma-ray production is at depths such that the photons suffer several Compton scatterings before escaping, degrading their energy down to the hard X-ray band (10s of keV). SWIFT/BAT is a very wide field coded mask instrument working in the energy band 14-190 keV and so is very well suited to the search for such gamma-rays. A retrospective search is being made in the BAT data for evidence for gamma-ray emission from the direction of novae at around the time of their explosion. So far the only positive detection is of RS Ophiuchi and in this case the emission is probably due to shock heating.

Skinner, Gerry↗
NASA NTRS2009

Adenosine Monophosphate-Based Detection of Bacterial Spores

A method of rapid detection of bacterial spores is based on the discovery that a heat shock consisting of exposure to a temperature of 100 C for 10 minutes causes the complete release of adenosine monophosphate (AMP) from the spores. This method could be an alternative to the method described in the immediately preceding article. Unlike that method and related prior methods, the present method does not involve germination and cultivation; this feature is an important advantage because in cases in which the spores are those of pathogens, delays involved in germination and cultivation could increase risks of infection. Also, in comparison with other prior methods that do not involve germination, the present method affords greater sensitivity. At present, the method is embodied in a laboratory procedure, though it would be desirable to implement the method by means of a miniaturized apparatus in order to make it convenient and economical enough to encourage widespread use.

Kern, Roger G.↗
NASA NTRS2009

Extraterrestrial Amino Acids in the Almahata Sitta Meteorite

Amino acid analysis of a meteorite fragment of asteroid 2008 TC(sub 3) called Almahata Sitta was carried out using reverse-phase high-perfo rmance liquid chromatography coupled with UV fluorescence detection a nd time-of-flight mass spectrometry (HPLC-FD/ToF-MS) as part of a sam ple analysis consortium. HPLC analyses of hot-water extracts from the meteorite revealed a complex distribution of two- to six-carbon aliph atic amino acids and one- to three carbon amines with abundances rang ing from 0.5 to 149 parts-per-billion (ppb). The enantiomeric ratios of the amino acids alanine, Beta-amino-n-butyric acid (Beta-ABA), 2-amino-2- methylbutanoic acid (isovaline), and 2-aminopentanoic acid (no rvaline) in the meteorite were racemic (D/L approximately 1), indicat ing that these amino acids are indigenous to the meteorite and not te rrestrial contaminants. Several other non-protein amino acids were also identified in the meteorite above background levels including alpha -aminoisobutyric acid (alpha-AIB), 4-amino-2- methybutanoic acid, 4-a mino-3-methylbutanoic acid, and 3-, 4-, and 5-aminopentanoic acid. Th e total abundances of isovaline and AlB in Almahata Sitta are approximately 1000 times lower than the abundances of these amino acids found in the CM carbonaceous meteorite Murchison. The extremely love abund ances and unusual distribution of five carbon amino acids in Almahata Sitta compared to Cl, CM, and CR carbonaceous meteorites and may be due to extensive thermal alteration of amino acids on the parent aster oid by partial melting during formation or impact shock heating.

Glavin, Daniel P.↗
NASA NTRS2010

Origin of the Central Constant Emission Component of Eta Carinae

The X-ray campaign observation of the wind-wind colliding (WWC) binary system, Eta Carinae, targeted at its periastron passage in 2003, presented a detailed view of the flux and spectral variations of the X-ray minimum phase. One of the discoveries in this campaign was a central constant emission (CCE) component very near the central WWC source (Hamaguchi et al. 2007, ApJ, 663, 522). The CCE component was noticed between 1-3 keY during the X-ray minima and showed no variation on either short timescales within any observation or long timescales of up to ~ 10 years. Hamaguchi et al. (2007) discussed possible origins as collisionally heated shocks from the fast polar winds from Eta Car or the fast moving outflow from the WWC with the ambient gas, or shocked gas that is intrinsic to the wind of Eta Car. During the 2009 periastron passage, we launched another focussed observing campaign of Eta Carinae with the Chandra, XMM-Newton and Suzaku observatories, concentrating on the X-ray faintest phase named the deep X-ray minimum. Thanks to multiple observations during the deep X-ray minimum, we found that the CCE spectrum extended up to 10 keV, indicating presence of hot plasma of kT approx.4-6 keV. This result excludes two possible origins that assume relatively slow winds (v approx. 1000 km/s) and only leaves the possibility that the CCE plasma is wind blown bubble at the WWC downstream. The CCE spectrum in 2009 showed a factor of 2 higher soft band flux as the CCE spectrum in 2003, while the hard band flux was almost unchanged. This variation suggests decrease in absorption column along the line of sight. We compare this result with recent increase in V-band magnitude of Eta Carinae and discuss location of the CCE plasma.

Hamaguchi, Kenji↗
NASA NTRS2010

Modeling Broadband X-Ray Absorption of Massive Star Winds

We present a method for computing the net transition of X-rays emitted by shock-heated plasma distributed throughout a partially optically thick stellar wind from a massive star. We find the transmission by an exact integration of the formal solution, assuming the emitting plasma and absorbing plasma are mixed at a constant mass ratio above some minimum radius, below which there is assumed to be no emission. This model is more realistic than either the slab absorption associated with a corona at the base of the wind or the exospheric approximation that assumes all observed X-rays are emitted without attenuation from above the radius of optical depth unity. Our model is implemented in XSPEC as a pre-calculated table that can be coupled to a user-defined table of the wavelength dependent wind opacity. We provide a default wind opacity model that is more representative of real wind opacities than the commonly used neutral ISM tabulation. Preliminary modeling of Chandra grating data indicates that the X-ray hardness trend of OB stars with spectral subtype cars largely be understood as a wind absorption effect.

Leutenegger, Maurice A.↗
NASA NTRS2010

Implications of a High Angular Resolution Image of the Sunyaev-Zel'Dovich Effect in RXJ1347-1145

The most X-ray luminous cluster known, RXJ1347-1145 (z = 0.45), has been the object of extensive study across the electromagnetic spectrum. We have imaged the Sunyaev-Zel'dovich effect (SZE) at 90 GHz (lambda = 33 mm) in RXJ1347-1145 at 10" resolution with the 64 pixel MUSTANG bolometer array on the Green Bank Telescope, confirming a previously reported strong, localized enhancement of the SZE 20" to the southeast of the center of X-ray emission. This enhancement of the SZE has been interpreted as shock-heated (>20keV) gas caused by an ongoing major (low mass ratio) merger event. Our data support this interpretation. We also detect a pronounced asymmetry in the projected cluster pressure profile, with the pressure just east of the cluster core approx. 1.6x higher than just to the west. This is the highest resolution image of the SZE made to date.

Mason, B. S.↗