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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 415 records · Page 23
All sourcesNASA NTRSDOE OSTI
NASA NTRS2002

Regaining Lost Separation in a Piloted Simulation of Autonomous Aircraft Operations

NASA is currently investigating a new concept of operations for the National Airspace System, designed to improve capacity while maintaining or improving current levels of safety. This concept, known as Distributed Air/Ground Traffic Management (DAG-TM), allows appropriately equipped autonomous aircraft to maneuver freely for flight optimization while resolving conflicts with other traffic and staying out of special use airspace and hazardous weather. While Airborne Separation Assurance System (ASAS) tools would normally allow pilots to resolve conflicts before they become hazardous, evaluation of system performance in sudden, near-term conflicts is needed in order to determine concept feasibility. If an acceptable safety level can be demonstrated in these situations, then operations may be conducted with lower separation minimums. An experiment was conducted in NASA Langley s Air Traffic Operations Lab to address issues associated with resolving near-term conflicts and the potential use of lower separation minimums. Sixteen commercial airline pilots flew a total of 32 traffic scenarios that required them to use prototype ASAS tools to resolve close range pop-up conflicts. Required separation standards were set at either 3 or 5 NM lateral spacing, with 1000 ft vertical separation being used for both cases. Reducing the lateral separation from 5 to 3 NM did not appear to increase operational risk, as indicated by the proximity to the intruder aircraft. Pilots performed better when they followed tactical guidance cues provided by ASAS than when they didn't follow the guidance. As air-air separation concepts are evolved, further studies will consider integration issues between ASAS and existing Airborne Collision Avoidance Systems (ACAS).These types of non-normal events will require the ASAS to provide effective alerts and resolutions prior to the time that an Airborne Collision Avoidance System (ACAS) would give a Resolution Advisory (RA). When an RA is issued, a pilot must take immediate action in order to avoid a potential near miss. The Traffic Alert and Collision Avoidance System (TCAS) II currently functions as an ACAS aboard commercial aircraft. Depending on the own aircraft s altitude, TCAS only issues RA s 15-35 seconds prior to the Closest Point of Approach (CPA). Prior to an RA, DAG-TM pilots operating autonomous aircraft must rely solely on ASAS for resolution guidance. An additional area of DAG-TM concept feasibility relates to a potential reduction in separation standards. Lower separation standards are likely needed in order to improve NAS efficiency and capacity. Current separation minimums are based in large part on the capabilities of older radar systems. Safety assessments are needed to determine the feasibility of reduced separation minimums. They will give strong consideration to surveillance system performance, including accuracy, integrity, and availability. Candidate surveillance systems include Automatic Dependent Surveillance-Broadcast (ADS-B) and multi-lateration systems. Considering studies done for Reduced Vertical Separation Minimums (RVSM) operations, it is likely that flight technical errors will also be considered. In addition to a thorough evaluation of surveillance system performance, a potential decision to lower the separation standards should also take operational considerations into account. An ASAS Safety Assessment study identified improper maneuvering in response to a conflict (due to ambiguous or improper resolution commands or a pilot s failure to comply with the resolution) as a potential safety risk. If near-term conflicts with lower separation minimums were determined to be more challenging for pilots, the severity of these risks could be even greater.

Barhydt, Richard↗
NASA NTRS2008

Using LDPC Code Constraints to Aid Recovery of Symbol Timing

A method of utilizing information available in the constraints imposed by a low-density parity-check (LDPC) code has been proposed as a means of aiding the recovery of symbol timing in the reception of a binary-phase-shift-keying (BPSK) signal representing such a code in the presence of noise, timing error, and/or Doppler shift between the transmitter and the receiver. This method and the receiver architecture in which it would be implemented belong to a class of timing-recovery methods and corresponding receiver architectures characterized as pilotless in that they do not require transmission and reception of pilot signals. Acquisition and tracking of a signal of the type described above have traditionally been performed upstream of, and independently of, decoding and have typically involved utilization of a phase-locked loop (PLL). However, the LDPC decoding process, which is iterative, provides information that can be fed back to the timing-recovery receiver circuits to improve performance significantly over that attainable in the absence of such feedback. Prior methods of coupling LDPC decoding with timing recovery had focused on the use of output code words produced as the iterations progress. In contrast, in the present method, one exploits the information available from the metrics computed for the constraint nodes of an LDPC code during the decoding process. In addition, the method involves the use of a waveform model that captures, better than do the waveform models of the prior methods, distortions introduced by receiver timing errors and transmitter/ receiver motions. An LDPC code is commonly represented by use of a bipartite graph containing two sets of nodes. In the graph corresponding to an (n,k) code, the n variable nodes correspond to the code word symbols and the n-k constraint nodes represent the constraints that the code places on the variable nodes in order for them to form a valid code word. The decoding procedure involves iterative computation of values associated with these nodes. A constraint node represents a parity-check equation using a set of variable nodes as inputs. A valid decoded code word is obtained if all parity-check equations are satisfied. After each iteration, the metrics associated with each constraint node can be evaluated to determine the status of the associated parity check. Heretofore, normally, these metrics would be utilized only within the LDPC decoding process to assess whether or not variable nodes had converged to a codeword. In the present method, it is recognized that these metrics can be used to determine accuracy of the timing estimates used in acquiring the sampled data that constitute the input to the LDPC decoder. In fact, the number of constraints that are satisfied exhibits a peak near the optimal timing estimate. Coarse timing estimation (or first-stage estimation as described below) is found via a parametric search for this peak. The present method calls for a two-stage receiver architecture illustrated in the figure. The first stage would correct large time delays and frequency offsets; the second stage would track random walks and correct residual time and frequency offsets. In the first stage, constraint-node feedback from the LDPC decoder would be employed in a search algorithm in which the searches would be performed in successively narrower windows to find the correct time delay and/or frequency offset. The second stage would include a conventional first-order PLL with a decision-aided timing-error detector that would utilize, as its decision aid, decoded symbols from the LDPC decoder. The method has been tested by means of computational simulations in cases involving various timing and frequency errors. The results of the simulations ined in the ideal case of perfect timing in the receiver.

Jones, Christopher↗
DOE OSTI2011

BLENDING STUDY FOR SRR SALT DISPOSITION INTEGRATION: TANK 50H SCALE-MODELING AND COMPUTER-MODELING FOR BLENDING PUMP DESIGN, PHASE 2

The Salt Disposition Integration (SDI) portfolio of projects provides the infrastructure within existing Liquid Waste facilities to support the startup and long term operation of the Salt Waste Processing Facility (SWPF). Within SDI, the Blend and Feed Project will equip existing waste tanks in the Tank Farms to serve as Blend Tanks where 300,000-800,000 gallons of salt solution will be blended in 1.3 million gallon tanks and qualified for use as feedstock for SWPF. Blending requires the miscible salt solutions from potentially multiple source tanks per batch to be well mixed without disturbing settled sludge solids that may be present in a Blend Tank. Disturbing solids may be problematic both from a feed quality perspective as well as from a process safety perspective where hydrogen release from the sludge is a potential flammability concern. To develop the necessary technical basis for the design and operation of blending equipment, Savannah River National Laboratory (SRNL) completed scaled blending and transfer pump tests and computational fluid dynamics (CFD) modeling. A 94 inch diameter pilot-scale blending tank, including tank internals such as the blending pump, transfer pump, removable cooling coils, and center column, were used in this research. The test tank represents a 1/10.85 scaled version of an 85 foot diameter, Type IIIA, nuclear waste tank that may be typical of Blend Tanks used in SDI. Specifically, Tank 50 was selected as the tank to be modeled per the SRR, Project Engineering Manager. SRNL blending tests investigated various fixed position, non-rotating, dual nozzle pump designs, including a blending pump model provided by the blend pump vendor, Curtiss Wright (CW). Primary research goals were to assess blending times and to evaluate incipient sludge disturbance for waste tanks. Incipient sludge disturbance was defined by SRR and SRNL as minor blending of settled sludge from the tank bottom into suspension due to blending pump operation, where the sludge level was shown to remain constant. To experimentally model the sludge layer, a very thin, pourable, sludge simulant was conservatively used for all testing. To experimentally model the liquid, supernate layer above the sludge in waste tanks, two salt solution simulants were used, which provided a bounding range of supernate properties. One solution was water (H{sub 2}O + NaOH), and the other was an inhibited, more viscous salt solution. The research performed and data obtained significantly advances the understanding of fluid mechanics, mixing theory and CFD modeling for nuclear waste tanks by benchmarking CFD results to actual experimental data. This research significantly bridges the gap between previous CFD models and actual field experiences in real waste tanks. A finding of the 2009, DOE, Slurry Retrieval, Pipeline Transport and Plugging, and Mixing Workshop was that CFD models were inadequate to assess blending processes in nuclear waste tanks. One recommendation from that Workshop was that a validation, or bench marking program be performed for CFD modeling versus experiment. This research provided experimental data to validate and correct CFD models as they apply to mixing and blending in nuclear waste tanks. Extensive SDI research was a significant step toward bench marking and applying CFD modeling. This research showed that CFD models not only agreed with experiment, but demonstrated that the large variance in actual experimental data accounts for misunderstood discrepancies between CFD models and experiments. Having documented this finding, SRNL was able to provide correction factors to be used with CFD models to statistically bound full scale CFD results. Through the use of pilot scale tests performed for both types of pumps and available engineering literature, SRNL demonstrated how to effectively apply CFD results to salt batch mixing in full scale waste tanks. In other words, CFD models were in error prior to development of experimental correction factors determined during this research, which provided a technique to use CFD models for salt batch mixing and transfer pump operations. This major scientific advance in mixing technology resulted in multi-million dollar cost savings to SRR. New techniques were developed for both experiment and analysis to complete this research. Supporting this success, research findings are summarized in the Conclusions section of this report, and technical recommendations for design and operation are included in this section of the report.

08 HYDROGEN↗
NASA NTRS1992

Situation assessment in the Paladin tactical decision generation system

Paladin is a real-time tactical decision generator for air combat engagements. Paladin uses specialized knowledge-based systems and other Artificial Intelligence (AI) programming techniques to address the modern air combat environment and agile aircraft in a clear and concise manner. Paladin is designed to provide insight into both the tactical benefits and the costs of enhanced agility. The system was developed using the Lisp programming language on a specialized AI workstation. Paladin utilizes a set of air combat rules, an active throttle controller, and a situation assessment module that have been implemented as a set of highly specialized knowledge-based systems. The situation assessment module was developed to determine the tactical mode of operation (aggressive, defensive, neutral, evasive, or disengagement) used by Paladin at each decision point in the air combat engagement. Paladin uses the situation assessment module; the situationally dependent modes of operation to more accurately represent the complex decision-making process of human pilots. This allows Paladin to adapt its tactics to the current situation and improves system performance. Discussed here are the details of Paladin's situation assessment and modes of operation. The results of simulation testing showing the error introduced into the situation assessment module due to estimation errors in positional and geometric data for the opponent aircraft are presented. Implementation issues for real-time performance are discussed and several solutions are presented, including Paladin's use of an inference engine designed for real-time execution.

Mcmanus, John W.↗
NASA NTRS2013

Single Pilot Workload Management During Cruise in Entry Level Jets

Advanced technologies and automation are important facilitators of single pilot operations, but they also contribute to the workload management challenges faced by the pilot. We examined task completion, workload management, and automation use in an entry level jet (ELJ) flown by single pilots. Thirteen certificated Cessna Citation Mustang (C510-S) pilots flew an instrument flight rules (IFR) experimental flight in a Cessna Citation Mustang simulator. At one point participants had to descend to meet a crossing restriction prior to a waypoint and prepare for an instrument approach into an un-towered field while facilitating communication from a lost pilot who was flying too low for ATC to hear. Four participants experienced some sort of difficulty with regard to meeting the crossing restriction and almost half (n=6) had problems associated with the instrument approach. Additional errors were also observed including eight participants landing at the airport with an incorrect altimeter setting.

Burian, Barbara K.↗
NASA NTRS2019

Shape Sensing for Wings with Spars and Ribs Using Simulated Strain

Active trim shape control can be used to minimize error between target and actual aircraft trim shape during flight. Trim shape sensing for aircraft during flight is not only important for highly flexible aircraft, such as the National Aeronautics and Space Administration (NASA) Helios Prototype remotely piloted flying wing aircraft, but also for a delta-wing type aircraft, such as a supersonic commercial transport aircraft. A two-step theory utilizing distributed strain for a real-time shape sensing of a full three-dimensional structure has been introduced previously. This study focuses on the application of the two-step theory to finite element models of a wing with spars and ribs such as the X-59 QueSST aircraft (Lockheed Martin Corporation, Bethesda, Maryland), a tapered wing, a dihedral/anhedral wing, and a stiffened dihedral/anhedral wing. A finely meshed finite element structural model is desired to capture accurate curvature distributions along the neutral axes of the wing cross sections during pre-test analysis for shape sensing of a wing with ribs and spars. The two-step theory used in this study gives excellent deformation correlation with the MSC/NASTRAN (MSC Software, Newport Beach, California) results along the neutral axis for all test cases used in this study except the X-59 QueSST aircraft.

strain↗
NASA NTRS2014

Trajectory Prediction Accuracy and Error Sources for Regional Jet Descents: Results of a 2010 Flight Trial at Denver International Airport using SkyWest Revenue Flights - Part II

The Efficient Descent Advisor (EDA) controller automation tool generates trajectory-based speed, path, and altitude-profile advisories to facilitate efficient, continuous descents into congested terminal airspace. While prior field trials have assessed the trajectory prediction accuracy for large jet (i.e., Boeing and Airbus) types, smaller (i.e., regional and business) jet types present unique challenges involving different descent procedures and Flight Management System (FMS) capabilities. This paper quantifies the trajectory prediction accuracy for small jet revenue flight descents based on SkyWest Canadair Regional Jet 200, 700, and 900 aircraft arrivals to Denver in the fall of 2010. Post flight test data analysis and SkyWest pilot interviews uncovered unexpected variation between flight crews due to different interpretations of (1) which fixed flight path angle (FPA) to fly based on the flight trial procedure and (2) how to fly the descent to achieve the target FPA. Pilot reports were used to select a subset of flights where pilots indicated an FPA according to the flight trial procedure to remove the unexpected variation due to (1) to focus on (2). Results for the subset for en-route descents, from prior to top of descent to the meter fix 30 to 130 nmi downstream, indicate that aircraft arrived to the meter fix six seconds early with about a twelve second standard deviation. Large FPA errors up to one degree relative to the EDA flight trial procedure were detected after the flight trial as a characteristic of the unexpected variation. It is recommended that quantitative validation be performed during future flight trials so that experimental procedures can be adjusted if unexpected results are detected.

Field Test Results↗
NASA NTRS1979

Capturing and tracking performance of the horizontal guidance and control systems of the terminal configured vehicle

A twin-jet commercial transport equipped with digital navigation, guidance, and control systems and advanced electronic display system was used for airborne operational research. The results of flight tests which evaluated a second-order horizontal-path guidance control law and the autopilot and airplane response are presented. This evaluation was accomplished through analysis of recorded flight data and through pilot opinion of the airplane maneuvers during automatic path-capture scenarios and path tracking. Four different path captures were flown at a ground speed of approximately 160 knots and repeated at approximately 300 knots. Path tracking tracking error was measured in terms of cross track error along two paths: one pat at cruise speeds and one at airport-terminal-area speeds. The path tracking accuracy and the smoothness of the airplane maneuvers were judged satisfactory for high speeds; however, at lower speeds the control law design should be improved so that tracking will be more accurate for operations in the airport terminal area.

Knox, C. E.↗
NASA NTRS1980

Active retrodirective arrays for SPS beam pointing

The basic requirement of the SPS beam pointing system is that it deliver a certain amount of S-band (lambda = 12.5 cm) power to a 9.6 km diameter receiving rectenna on the ground. The power is transmitted from a 1.0 km diameter antenna array on the SPS, which is, for a rectenna at about plus or minus 40 deg. latitude, some 37.5x10 to the 6th power km distant. At the present time ARA's appear to be the best bet to realize this very stringent beam pointing requirement. An active retrodirective array (ARA) transmits a beam towards the apparent source of an illuminating signal called the pilot. The array produces, not merely reflects, RF power. Retrodirectivity is achieved by retransmitting from each element of the array a signal whose phase is the "conjugate" of that received by the element. Phase conjugate circuits and pointing errors in ARA's are described. Results obtained using a 2-element X-band ARA and an 8-element S-band ARA are included.

Chernoff, R.↗
NASA NTRS2013

Understanding Crew Decision-Making in the Presence of Complexity: A Flight Simulation Experiment

Crew decision making and response have long been leading causal and contributing factors associated with aircraft accidents. Further, it is anticipated that future aircraft and operational environments will increase exposure to risks related to these factors if proactive steps are not taken to account for ever-increasing complexity. A flight simulation study was designed to collect data to help in understanding how complexity can, or may, be manifest. More specifically, an experimental apparatus was constructed that allowed for manipulation of information complexity and uncertainty, while also manipulating operational complexity and uncertainty. Through these manipulations, and the aid of experienced airline pilots, several issues have been discovered, related most prominently to the influence of information content, quality, and management. Flight crews were immersed in an environment that included new operational complexities suggested for the future air transportation system as well as new technological complexities (e.g. electronic flight bags, expanded data link services, synthetic and enhanced vision systems, and interval management automation). In addition, a set of off-nominal situations were emulated. These included, for example, adverse weather conditions, traffic deviations, equipment failures, poor data quality, communication errors, and unexpected clearances, or changes to flight plans. Each situation was based on one or more reference events from past accidents or incidents, or on a similar case that had been used in previous developmental tests or studies. Over the course of the study, 10 twopilot airline crews participated, completing over 230 flights. Each flight consisted of an approach beginning at 10,000 ft. Based on the recorded data and pilot and research observations, preliminary results are presented regarding decision-making issues in the presence of the operational and technological complexities encountered during the flights.

Young, Steven D.↗
NASA NTRS1994

Certification for civil flight decks and the human-computer interface

This paper will address the issue of human factor aspects of civil flight deck certification, with emphasis on the pilot's interface with automation. In particular, three questions will be asked that relate to this certification process: (1) are the methods, data, and guidelines available from human factors to adequately address the problems of certifying as safe and error tolerant the complex automated systems of modern civil transport aircraft; (2) do aircraft manufacturers effectively apply human factors information during the aircraft flight deck design process; and (3) do regulatory authorities effectively apply human factors information during the aircraft certification process?

Mcclumpha, Andrew J.↗
NASA NTRS1993

Distributed problem solving by pilots and dispatchers

The study addressed the following question: Are flight planning problems solved differently by PILOTS and DISPATCHERS when they work alone versus when they work together? Aspect of their performance that were of interest include the following: Problem perception and definition; Problem solving strategies and information use; Options considered; Solution and rational; and errors.

Orasanu, Judith↗
NASA NTRS1993

Automation design and crew coordination

Advances in technology have greatly impacted the appearance of the modern aircraft cockpit. Where once one would see rows upon rows. The introduction of automation has greatly altered the demands on the pilots and the dynamics of aircrew task performance. While engineers and designers continue to implement the latest technological innovations in the cockpit - claiming higher reliability and decreased workload - a large percentage of aircraft accidents are still attributed to human error. Rather than being the main instigators of accidents, operators tend to be the inheritors of system defects created by poor design, incorrect installation, faulty maintenance and bad management decisions. This paper looks at some of the variables that need to be considered if we are to eliminate at least one of these inheritances - poor design. Specifically, this paper describes the first part of a comprehensive study aimed at identifying the effects of automation on crew coordination.

Segal, Leon D.↗
NASA NTRS2019

Usability Evaluation of Indicators of Energy-Related Problems in Commercial Airline Flight Decks

A series of pilot-in-the-loop flight simulation studies were conducted at NASA Langley Research Center to evaluate indicators aimed at supporting the flight crew’s awareness of problems related to energy states. Indicators were evaluated utilizing state-of-the-art flight deck systems such as on commercial air transport aircraft. This paper presents results for four technologies: (1) conventional primary flight display speed cues, (2) an enhanced airspeed control indicator, (3) a synthetic vision baseline that provides a flight path vector, speed error, and an acceleration cue, and (4) an aural airspeed alert that triggers when current airspeed deviates beyond a specified threshold from the selected airspeed. Full-mission high-fidelity flight simulation studies were conducted using commercial airline crews. Crews were paired by airline for common crew resource management procedures and protocols. Scenarios spanned a range of complex conditions while emulating several causal factors reported in recent accidents involving loss of energy state awareness by pilots. Data collection included questionnaires administered at the completion of flight scenarios, aircraft state data, audio/video recordings of flight crew, eye tracking, pilot control inputs, and researcher observations. Questionnaire response data included subjective measures of workload, situation awareness, complexity, usability, and acceptability. This paper reports relevant findings derived from subjective measures as well as quantitative measures.

Evans, Emory T.↗
NASA NTRS2010

A GPS-Based Pitot-Static Calibration Method Using Global Output-Error Optimization

Pressure-based airspeed and altitude measurements for aircraft typically require calibration of the installed system to account for pressure sensing errors such as those due to local flow field effects. In some cases, calibration is used to meet requirements such as those specified in Federal Aviation Regulation Part 25. Several methods are used for in-flight pitot-static calibration including tower fly-by, pacer aircraft, and trailing cone methods. In the 1990 s, the introduction of satellite-based positioning systems to the civilian market enabled new inflight calibration methods based on accurate ground speed measurements provided by Global Positioning Systems (GPS). Use of GPS for airspeed calibration has many advantages such as accuracy, ease of portability (e.g. hand-held) and the flexibility of operating in airspace without the limitations of test range boundaries or ground telemetry support. The current research was motivated by the need for a rapid and statistically accurate method for in-flight calibration of pitot-static systems for remotely piloted, dynamically-scaled research aircraft. Current calibration methods were deemed not practical for this application because of confined test range size and limited flight time available for each sortie. A method was developed that uses high data rate measurements of static and total pressure, and GPSbased ground speed measurements to compute the pressure errors over a range of airspeed. The novel application of this approach is the use of system identification methods that rapidly compute optimal pressure error models with defined confidence intervals in nearreal time. This method has been demonstrated in flight tests and has shown 2- bounds of approximately 0.2 kts with an order of magnitude reduction in test time over other methods. As part of this experiment, a unique database of wind measurements was acquired concurrently with the flight experiments, for the purpose of experimental validation of the optimization method. This paper describes the GPS-based pitot-static calibration method developed for the AirSTAR research test-bed operated as part of the Integrated Resilient Aircraft Controls (IRAC) project in the NASA Aviation Safety Program (AvSP). A description of the method will be provided and results from recent flight tests will be shown to illustrate the performance and advantages of this approach. Discussion of maneuver requirements and data reduction will be included as well as potential applications.

Foster, John V.↗
NASA NTRS1989

Piloted simulation of a ground-based time-control concept for air traffic control

A joint simulation was carried out using a piloted simulator and an advanced-concept air traffic control simulation to study the acceptability and accuracy of the ground-based four-dimensional descent advisor (DA), an automation aid based on accurate models of aircraft performance and weather conditions. In the piloted simulation, airline crews executed controller-issued descent advisories along standard curved-path arrival routes and were able to achieve an arrival-time precision of plus or minus 20 s at the metering fix. An analysis of errors generated in turns resulted in a further enhancement of the DA algorithm.

Davis, Thomas J.↗
NASA NTRSDate not supplied

The Contribution of Pilots to Resilience in Normal Operations. Part II: A closer look at briefings: Anticipation and Monitoring Also Known as Planning and Coordination

Much of our knowledge about human performance in flight safety has come from the analysis of undesired events, whether accidents, incidents, or crew behaviors identified via flight exceedance monitoring or observational techniques. In recent years, there has been an acknowledgement that operational personnel are not merely sources of “human error”, but also make a unique human contribution to safe outcomes. In a few celebrated cases, this takes the form of “heroic saves”, but on many more occasions, operational personnel contribute to safety through everyday, often-unnoticed actions that turn potentially hazardous situations into non-events. An emerging approach to safety, frequently referred to as “Safety II,” proposes that the positive human contribution is an important and largely untapped source of safety information. Some airlines have successfully trained observers to identify and record the positive behaviors exhibited by the crew over the course of a flight. In other cases, flight crew are interviewed about good practices or positive behaviors. However, each of these methods are relatively limited in scale and resource intensive. A survey could provide a relatively low-cost approach to systematically gather this information on a larger scale. The primary purpose of the research was to develop and assess a surveys methodology for assessing crews' activities in normal flights and the operational perturbations encountered during normal operations. We hope that such a survey could be both a research tool as well as a safety management aid for the aviation industry. We collected responses concerning revenue flights from two groups of airline pilots (N = 25 & N= 65). The results indicated that relatively few flights proceeded exactly as in the original flight plan. Pilots routinely anticipated and adapted to changing circumstances. We will review the challenges encountered in developing the survey and summarize preliminary findings from two administrations of the survey to airline pilots.

human contribution safety↗
DOE OSTI2020

Potential for Applying Continuous Directed Evolution to Plant Enzymes: An Exploratory Study

Plant evolution has produced enzymes that may not be optimal for maximizing yield and quality in today’s agricultural environments and plant biotechnology applications. By improving enzyme performance, it should be possible to alleviate constraints on yield and quality currently imposed by kinetic properties or enzyme instability. Enzymes can be optimized more quickly than naturally possible by applying directed evolution, which entails mutating a target gene in vitro and screening or selecting the mutated gene products for the desired characteristics. Continuous directed evolution is a more efficient and scalable version that accomplishes the mutagenesis and selection steps simultaneously in vivo via error-prone replication of the target gene and coupling of the host cell’s growth rate to the target gene’s function. However, published continuous systems require custom plasmid assembly, and convenient multipurpose platforms are not available. We discuss two systems suitable for continuous directed evolution of enzymes, OrthoRep in Saccharomyces cerevisiae and EvolvR in Escherichia coli, and our pilot efforts to adapt each system for high-throughput plant enzyme engineering. To test our modified systems, we used the thiamin synthesis enzyme THI4, previously identified as a prime candidate for improvement. Our adapted OrthoRep system shows promise for efficient plant enzyme engineering.

59 BASIC BIOLOGICAL SCIENCES↗