Remediation of Irradiated Graphite Using Microorganisms Isolated from Oil Contaminated Soil - 19643
The deployment of nuclear energy globally is hindered by the fear cleared by nuclear reactor disasters such as the Chernobyl and Fukushima reactor meltdowns. Additionally, accumulation of radioactive waste globally will, in the near future, present a significant threat to humans and the environment. Notably, the majority of nuclear waste from the energy generation industry is generated as Low Level Waste (LLW) in which the small radioactive component renders the bulk of the waste unsuitable for conventional treatment and disposal. In the case of radioactive graphite, the small radioactive component comprising radiocarbon-14 (C-14) and metallic pollutants can be removed to allow safe disposal or reuse as of the graphite. In the current study, mixed-cultures of organisms isolated from oil contaminated sites showed potential of selective bioaccumulation of C-14 from a powdered C-12/C-14 graphite matrix. The study was conducted in a continuously mixed reactor (growth vessel) constructed from Pyrex panels (30 cm x 20 cm x 15 cm) with finely ground graphite as the principal carbon source (1500-2000 mg.L{sup -1}) and spikes of glucose (500 mg.L{sup -1} every 48 hours). The growth vessel was connected to a biofilter unit by submersible pump where particulate matter was isolated for further analysis. Results showed that approximately 68% more C-14 was released from ignited dry cells than from respiration product. C-14 accumulated in cells corrected using a trap filter system, with a marked decrease of C-14 in carbon dioxide than vegetative suspended cells in the main tank. The only mechanism that could account for the differential C-14 distribution between the liquid medium and cells was possible fractionation during uptake of carbon compounds. The discriminating factor suggested in this study is the kinetic isotope effect (KIE) which allows heavier atoms to have different affinities for reaction sites on catalysis and biocatalysis. (authors)