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At least 433 records · Page 24

Near Earth Asteroid Solar Sail Engineering Development Unit Test Program

The Near Earth Asteroid (NEA) Scout project is a 30x20x10cm (6U) cubesat reconnaissance mission to investigate a near Earth asteroid utilizing an 86m2 solar sail as the primary propulsion system. This will be the largest solar sail NASA will launch to date. NEA Scout is a secondary payload currently manifested on the maiden voyage of the Space Launch System in 2018. In development of the solar sail subsystem, design challenges were identified and investigated for packaging within a 6U form factor and deployment in cis-lunar space. Analysis furthered understanding of thermal, stress, and dynamics of the stowed system and matured an integrated sail membrane model for deployed flight dynamics. This paper will address design, fabrication, and lessons learned from the NEA Scout solar sail subsystem engineering development unit. From optical properties of the sail material to folding and spooling the single 86m2 sail, the team has developed a robust deployment system for the solar sail. This paper will also address expected and received test results from ascent vent, random vibration, and deployment tests.

Lockett, Tiffany Russell↗

CO2 Removal and Atmosphere Revitalization Systems for Next Generation Space Flight

Removal of metabolic CO2 from breathing air is a vital process for life support in all crewed space missions. A CO2 removal processor called the Low Power CO2 Removal (LPCOR) system is being developed in the Bioengineering Branch at NASA Ames Research Center. LPCOR utilizes advanced adsorption and membrane gas separation processes to achieve substantial power and mass reduction when compared to the state-of-the-art carbon dioxide removal assembly (CORA) of the US segment of the International Space Station (ISS). LPCOR is an attractive alternative for use in commercial spacecraft for short-duration missions and can easily be adapted for closed-loop life support applications. NASA envisions a next-generation closed-loop atmosphere revitalization system that integrates advanced CO2 removal, O2 recovery, and trace contaminant control processes to improve overall system efficiency. LPCOR will serve as the front end to such a system. LPCOR is a reliable air revitalization technology that can serve both the near-term and long-term human space flight needs of NASA and its commercial partners.

Luna, Bernadette↗

NASA Tech Briefs, May 2003

Topics covered include: Using Diffusion Bonding in Making Piezoelectric Actuators; Wireless Temperature-Monitoring System; Analog Binaural Circuits for Detecting and Locating Leaks; Mirrors Containing Biomimetic Shape-Control Actuators; Surface-Micromachined Planar Arrays of Thermopiles; Cascade Back-Propagation Learning in Neural Networks; Perovskite Superlattices as Tunable Microwave Devices; Rollable Thin-Shell Nanolaminate Mirrors; Flight Tests of a Ministick Controller in an F/A-18 Airplane; Piezoelectrically Actuated Shutter for High Vacuum; Bio-Inspired Engineering of Exploration Systems; Microscope Cells Containing Multiple Micromachined Wells; Electrophoretic Deposition for Fabricating Microbatteries; Integrated Arrays of Ion-Sensitive Electrodes; Model of Fluidized Bed Containing Reacting Solids and Gases; Membrane Mirrors With Bimorph Shape Actuators; Using Fractional Clock-Period Delays in Telemetry Arraying; Developing Generic Software for Spacecraft Avionics; Numerical Study of Pyrolysis of Biomass in Fluidized Beds; and Assessment of Models of Chemically Reacting Granular Flows.

Source record↗

A Solar Sail Shape Modeling Approach for Attitude Control Design and Analysis

Solar sails operating in the space environment experience deformations in sail shape that result in relatively large disturbance torques which dictate the required performance of the spacecraft attitude control and momentum management systems. These deformations are driven by thermal loads on the booms (due to uneven solar heating), manufacturing and assembly tolerances, and variations in membrane tension. The Solar Cruiser spacecraft utilizes a four-quadrant sail design with four 30-meter length booms and four triangular sail membranes, creating a square sail structure of >1600 m2. Medium-fidelity mesh models were developed based on a characteristic deformed shape. A series of parametric studies were conducted using this shape paradigm to determine worst-case deformed sail shapes which produce bounding disturbance torques. A large database of shapes was produced, and the forces and moments induced by each individual shape were calculated using a Rios-Reyes reduced order generalized sail model. Two were selected as reference worst-case shapes for the Solar Cruiser mission: one which produced the highest pitch/yaw root-sum-squared (RSS) torque, and one which produced the highest roll torque. The results showed that the worst-case shapes at high solar incidence angles induce significantly higher (2-10x) disturbance torques than an ideal, flat-plate sail. Even with considerable safety margins, assuming an ideal sail is unlikely to sufficiently bound the disturbances, which is critical when designing the attitude control system and sizing actuators. Accurate sail shape modeling methodologies should therefore be employed on future solar sail missions.

Solar Sail↗

A Solar Sail Shape Modeling Approach for Attitude Control Design and Analysis

Solar sails operating in the space environment experience deformations in sail shape that result in relatively large disturbance torques which dictate the required performance of the spacecraft attitude control and momentum management systems. These deformations are driven by thermal loads on the booms (due to uneven solar heating), manufacturing and assembly tolerances, and variations in membrane tension. The Solar Cruiser spacecraft utilizes a four-quadrant sail design with four 30-meter length booms and four triangular sail membranes, creating a square sail structure of >1600 m2. Medium-fidelity mesh models were developed based on a characteristic deformed shape. A series of parametric studies were conducted using this shape paradigm to determine worst-case deformed sail shapes which produce bounding disturbance torques. A large database of shapes was produced, and the forces and moments induced by each individual shape were calculated using a Rios-Reyes reduced order generalized sail model [1]. Two were selected as reference worst-case shapes for the Solar Cruiser mission: one which produced the highest pitch/yaw root-sum-squared (RSS) torque, and one which produced the highest roll torque. The results showed that the worst-case shapes at high solar incidence angles induce significantly higher (2-10x) disturbance torques than an ideal, flat-plate sail. Even with considerable safety margins, assuming an ideal sail is unlikely to sufficiently bound the disturbances, which is critical when designing the attitude control system and sizing actuators. Accurate sail shape modeling methodologies should therefore be employed on future solar sail missions. References [1] L. Rios-Reyes and D. J. Scheeres, “Generalized Model for Solar Sails,” Journal of Spacecraft and Rockets, Vol. 42, Jan. 2005, pp. 182–185, 10.2514/1.9054.

Solar Sail↗

Blended-Wing-Body (BWB) Fuselage Structural Design for Weight Reduction

Structural analysis and design of efficient pressurized fuselage configurations for the advanced Blended-Wing-Body (BWB) flight vehicle is a challenging problem. Unlike a conventional cylindrical pressurized fuselage, stress level in a box type BWB fuselage is an order of magnitude higher, because internal pressure primarily results in bending stress instead of skin-membrane stress. In addition, resulting deformation of aerodynamic surface could significantly affect performance advantages provided by lifting body. The pressurized composite conformal multi-lobe tanks of X-33 type space vehicle also suffered from similar problem. In the earlier BWB design studies, Vaulted Ribbed Shell (VLRS), Flat Ribbed Shell (FRS); Vaulted shell Honeycomb Core (VLHC) and Flat sandwich shell Honeycomb Core (FLHC) concepts were studied. The flat and vaulted ribbed shell concepts were found most efficient. In a recent study, a set of composite sandwich panel and cross-ribbed panel were analyzed. Optimal values of rib and skin thickness, rib spacing, and panel depth were obtained for minimal weight under stress and buckling constraints. In addition, a set of efficient multi-bubble fuselage (MBF) configuration concept was developed. The special geometric configuration of this concept allows for balancing internal cabin pressure load efficiently, through membrane stress in inner-stiffened shell and inter-cabin walls, while the outer-ribbed shell prevents buckling due to external resultant compressive loads. The initial results from these approximate finite element analyses indicate progressively lower maximum stresses and deflections compared to the earlier study. However, a relative comparison of the FEM weight per unit floor area of the segment unit indicates that the unit weights are still relatively higher that the conventional B777 type cylindrical or A380 type elliptic fuselage design. Due to the manufacturing concern associated with multi-bubble fuselage, a Y braced box-type fuselage alternative with special resin-film injected (RFI) stitched carbon composite with foam-core was designed by Boeing under a NASA research contract for the 480 passenger version. It is shown that this configuration can be improved to a modified multi-bubble fuselage which has better stress distribution, for same material and dimension.

Mukhopadhyay, V.↗

Life without water

Anhydrobiosis, or life without water is commonly demonstrated by a number of plants and animals. These organisms have the capacity to loose all body water, remain dry for various periods, and then be revived by rehydration. While in the anhydrobiotic state, these organisms become highly resistant to several environmental stresses such as extremely low temperatures, elevated temperatures, ionizing radiation, and high vacuum. Since water is commonly thought to be essential for life, survival of anhydrobiotic organisms with an almost total loss of water is examined. A search of literature reveal that many anhydrobiotic organisms make large quantities of trehalose or other carbohydrates. Laboratory experiments have shown that trehalose is able to stabilize and preserve microsomes of sarcoplasmic reticulum and artificial liposomes. It was demonstrated that trehalose and other disaccharides can interact directly with phosopipid headgroups and maintain membranes in their native configuration by replacing water in the headgroup region. Recent studies show that trehalose is an effective stabilizer of proteins during drying and that it does so by direct interaction with groups on the protein. If life that is able to withstand environmental extremes has ever developed on Mars, it is expected that such life would have developed some protective compounds which can stabilize macromolecular structure in the absence of water and at cold temperatures. On Earth, that role appears to be filled by carbohydrates that can stabilize both membrane and protein stuctures during freezing and drying. By analog with terrestrial systems, such life forms might develop resistance either during some reproductive stage or at any time during adult existence. If the resistant form is a developmental stage, the life cycle of the organism must be completed with a reasonable time period relative to time when environmental conditions are favorable. This would suggest that simple organisms with a short life cycle might be most sucessful.

Crowe, Lois M.↗

Development of Modal Test Techniques for Validation of a Solar Sail Design

This paper focuses on the development of modal test techniques for validation of a solar sail gossamer space structure design. The major focus is on validating and comparing the capabilities of various excitation techniques for modal testing solar sail components. One triangular shaped quadrant of a solar sail membrane was tested in a 1 Torr vacuum environment using various excitation techniques including, magnetic excitation, and surface-bonded piezoelectric patch actuators. Results from modal tests performed on the sail using piezoelectric patches at different positions are discussed. The excitation methods were evaluated for their applicability to in-vacuum ground testing and to the development of on orbit flight test techniques. The solar sail membrane was tested in the horizontal configuration at various tension levels to assess the variation in frequency with tension in a vacuum environment. A segment of a solar sail mast prototype was also tested in ambient atmospheric conditions using various excitation techniques, and these methods are also assessed for their ground test capabilities and on-orbit flight testing.

Gaspar, James L.↗

Emerging Fuel Cell Technology Being Developed: Offers Many Benefits to Air Vehicles

Fuel cells, which have recently received considerable attention for terrestrial applications ranging from automobiles to stationary power generation, may enable new aerospace missions as well as offer fuel savings, quiet operations, and reduced emissions for current and future aircraft. NASA has extensive experience with fuel cells, having used them on manned space flight systems over four decades. Consequently, the NASA Glenn Research Center has initiated an effort to investigate and develop fuel cell technologies for multiple aerospace applications. Two promising fuel cell types are the proton exchange membrane (PEM) and solid oxide fuel cell (SOFC). PEM technology, first used on the Gemini spacecraft in the sixties, remained unutilized thereafter until the automotive industry recently recognized the potential. PEM fuel cells are low-temperature devices offering quick startup time but requiring relatively pure hydrogen fuel. In contrast, SOFCs operate at high temperatures and tolerate higher levels of impurities. This flexibility allows SOFCs to use hydrocarbon fuels, which is an important factor considering our current liquid petroleum infrastructure. However, depending on the specific application, either PEM or SOFC can be attractive. As only NASA can, the Agency is pursuing fuel cell technology for civil uninhabited aerial vehicles (UAVs) because it offers enhanced scientific capabilities, including enabling highaltitude, long-endurance missions. The NASA Helios aircraft demonstrated altitudes approaching 100,000 ft using solar power in 2001, and future plans include the development of a regenerative PEM fuel cell to provide nighttime power. Unique to NASA's mission, the high-altitude aircraft application requires the PEM fuel cell to operate on pure oxygen, instead of the air typical of terrestrial applications.

Walker, James F.↗

Curved-Focal-Plane Arrays Using Deformed-Membrane Photodetectors

A versatile and simple approach to the design and fabrication of curved-focal-plane arrays of silicon-based photodetectors is being developed. This approach is an alternative to the one described in "Curved Focal-Plane Arrays Using Back- Illuminated High-Purity Photodetectors" (NPO-30566), NASA Tech Briefs, Vol. 27, No. 10 (October 2003), page 10a. As in the cited prior article, the basic idea is to improve the performance of an imaging instrument and simplify the optics needed to obtain a given level of performance by making an image sensor (in this case, an array of photodetectors) conform to a curved focal surface, instead of designing the optics to project an image onto a flat focal surface. There is biological precedent for curved-focal-surface designs: retinas - the image sensors in eyes - conform to the naturally curved focal surfaces of eye lenses. The present approach is applicable to both front-side- and back-side-illuminated, membrane photodetector arrays and is being demonstrated on charge-coupled devices (CCDs). The very-large scale integrated (VLSI) circuitry of such a CCD or other array is fabricated on the front side of a silicon substrate, then the CCD substrate is attached temporarily to a second substrate for mechanical support, then material is removed from the back to obtain the CCD membrane, which typically has a thickness between 10 and 20 m. In the case of a CCD designed to operate in back-surface illumination, delta doping can be performed after thinning to enhance the sensitivity. This approach is independent of the design and method of fabrication of the front-side VLSI circuitry and does not involve any processing of a curved silicon substrate. In this approach, a third substrate would be prepared by polishing one of its surfaces to a required focal-surface curvature. A CCD membrane fabricated as described above would be pressed against, deformed into conformity with, and bonded to, the curved surface. The technique used to press and bond the CCD membrane would depend on the nature of the supporting material (see figure). For example, if the third substrate were made of quartz frit, the substrate would be prepared by suffusing it with epoxy. Then one would take advantage of the porosity of the frit by applying a partial vacuum to the opposite surface of the frit, causing atmospheric pressure to push the CCD membrane against the curved surface. The curing of the epoxy would bond the CCD membrane to the curved surface. Alternatively, if the third substrate were made of a nonporous material, the curved substrate surface would be prepared by coating it with a wax or an uncured epoxy. The CCD membrane would be pressed against the coated, curved surface by use of a suitably pressurized balloon. The CCD membrane would then become bonded to the curved surface by curing of the epoxy or freezing of the wax.

Nikzad, Shouleh↗

Findings from the Supersonic Qualification Program of the Mars Science Laboratory Parachute System

In 2012, the Mars Science Laboratory Mission (MSL) will deploy NASA's largest extra-terrestrial parachute, a technology integral to the safe landing of its advanced robotic explorer on the surface. The supersonic parachute system is a mortar deployed 21.5 m disk-gap-band (DGB) parachute, identical in geometric scaling to the Viking era DGB parachutes of the 1970's. The MSL parachute deployment conditions are Mach 2.3 at a dynamic pressure of 750 Pa. The Viking Balloon Launched Decelerator Test (BLDT) successfully demonstrated a maximum of 700 Pa at Mach 2.2 for a 16.1 m DGB parachute in its AV4 flight. All previous Mars deployments have derived their supersonic qualification from the Viking BLDT test series, preventing the need for full scale high altitude supersonic testing. The qualification programs for Mars Pathfinder, Mars Exploration Rover, and Phoenix Scout Missions were all limited to subsonic structural qualification, with supersonic performance and survivability bounded by the BLDT qualification. The MSL parachute, at the edge of the supersonic heritage deployment space and 33% larger than the Viking parachute, accepts a certain degree of risk without addressing the supersonic environment in which it will deploy. In addition, MSL will spend up to 10 seconds above Mach 1.5, an aerodynamic regime that is associated with a known parachute instability characterized by significant canopy projected area fluctuation and dynamic drag variation. This aerodynamic instability, referred to as "area oscillations" by the parachute community has drag performance, inflation stability, and structural implications, introducing risk to mission success if not quantified for the MSL parachute system. To minimize this risk and as an alternative to a prohibitively expensive high altitude test program, a multi-phase qualification program using computation simulation validated by subscale test was developed and implemented for MSL. The first phase consisted of 2% of fullscale supersonic wind tunnel testing of a rigid DGB parachute with entry-vehicle to validate two high fidelity computational fluid dynamics (CFD) tools. The computer codes utilized Large Eddy Simulation and Detached Eddy Simulation numerical approaches to accurately capture the turbulent wake of the entry vehicle and its coupling to the parachute bow-shock. The second phase was the development of fluid structure interaction (FSI) computational tools to predict parachute response to the supersonic flow field. The FSI development included the integration of the CFD from the first phase with a finite element structural model of the parachute membrane and cable elements. In this phase, a 4% of full-scale supersonic flexible parachute test program was conducted to provide validation data to the FSI code and an empirical dataset of the MSL parachute in a flight-like environment. The final phase is FSI simulations of the full-scale MSL parachute in a Mars type deployment. Findings from this program will be presented in terms of code development and validation, empirical findings from the supersonic testing, and drag performance during supersonic operation.

EDL↗

Systems Analysis Developed for All-Electric Aircraft Propulsion

There is a growing interest in the use of fuel cells as a power source for all-electric aircraft propulsion as a means to substantially reduce or eliminate environmentally harmful emissions. Among the technologies under consideration for these concepts are advanced proton exchange membrane (PEM) and solid oxide fuel cells (SOFCs), alternative fuels and fuel processing, and fuel storage. A multidisciplinary effort is underway at the NASA Glenn Research Center to develop and evaluate concepts for revolutionary, nontraditional fuel cell power and propulsion systems for aircraft applications. As part of this effort, system studies are being conducted to identify concepts with high payoff potential and associated technology areas for further development. To support this effort, a suite of component models was developed to estimate the mass, volume, and performance for a given system architecture. These models include a hydrogen-air PEM fuel cell; an SOFC; balance-of-plant components (compressor, humidifier, separator, and heat exchangers); compressed gas, cryogenic, and liquid fuel storage tanks; and gas turbine/generator models for hybrid system applications. First-order feasibility studies were completed for an all-electric personal air vehicle utilizing a fuel-cell-powered propulsion system. A representative aircraft with an internal combustion engine was chosen as a baseline to provide key parameters to the study, including engine power and subsystem mass, fuel storage volume and mass, and aircraft range. The engine, fuel tank, and associated ancillaries were then replaced with a fuel cell subsystem. Various configurations were considered including a PEM fuel cell with liquid hydrogen storage, a direct methanol PEM fuel cell, and a direct internal reforming SOFC/turbine hybrid system using liquid methane fuel. Each configuration was compared with the baseline case on a mass and range basis.

Kohout, Lisa L.↗

Power Systems Evaluated for Solar Electric Propulsion Vehicles

Solar electric propulsion (SEP) mission architectures are applicable to a wide range NASA missions including the robotic exploration of the outer planets in the next decade and the human exploration of Mars within the next 2 decades. SEP enables architectures that are very mass efficient with reasonable power levels (1-MW class) aerobrake and cryogenic upper-stage transportation technologies are utilized. In this architecture, the efficient SEP stage transfers the payload from low Earth orbit (LEO) High Energy Elliptical Parking Orbit (HEEPO) within a period of 6 to 12 months. highthrust, cryogenic upper stage and payload then separate from the SEP vehicle for injection to the planetary target, allowing for fast heliocentric trip times. This mission architecture offers a potential reduction in mass to LEO in comparison to alternative all-chemical nuclear propulsion schemes. Mass reductions may allow launch vehicle downsizing enable missions that would have been grounded because of cost constraints. The preceding figure illustrates a conceptual SEP stage design for a human Mars mission. Researchers at the NASA Glenn Research Center at Lewis Field designed conceptual SEP vehicle, conceived the mission architecture to use this vehicle, and analyzed the vehicle s performance. This SEP stage has a dry mass of 35 metric tons (MT), 40 MT of xenon propellant, and a photovoltaic array that spans 110 m, providing power to a cluster of eight 100-kW Hall thrusters. The stage can transfer an 80-MT payload and upper stage to the desired HEEPO. Preliminary packaging studies show this space-station-class SEP vehicle meets the proposed "Magnum" launch vehicle and volume requirements with considerable margin. An SEP vehicle for outer planetary missions, such as the Europa Mapper Mission, would be dramatically smaller than human Mars mission SEP stage. In this mission architecture, the SEP power system with the payload to provide spacecraft power throughout the mission. Several photovoltaic array design concepts were considered for the SEP vehicle power system for the human mission to Mars. These include a space station derivative, a SCARLET (Solar Concentrator Arrays with Refractive Linear Element Technology) derivative, and a hybrid inflatable-deployable thin polymer membrane array with thin-film solar cells (as shown in the concept illustration). This concept is based on a design developed for the Next Generation Space Telescope Sun shield. The array is divided into 16 independent electrical sections with 500-V, negative-grounded solar cell strings. The power system employs a channelized, 500-Vdc power management and distribution (PMAD) architecture with lithium ion batteries for energy storage for vehicle and payload secondary loads (the high-power Hall thrusters do not operate in eclipse periods). The 500-V PMAD voltage permits "direct-drive" thruster operation, greatly reducing the power processing unit size, complexity, and power loss. Similar power system architecture, designs, and technology are assumed for the Europa Mapper Mission SEP vehicle. The primary exceptions are that the photovoltaic array is assumed to consist of two rectangular wings and that the power system rating is 15 kW in Earth orbit and 200 W at Europa. To size the SEP vehicle power system, a dedicated Fortran code was developed to predict detailed power system performance, mass, and thermal control requirements. This code also modeled all the relevant Earth orbit environments; that is, the particulate radiation, plasma, meteoroids and debris, ultraviolet radiation, contamination, and thermal conditions. Analysis results for the Human Mars Mission SEP vehicle show a power system mass of 9-MT and photovoltaic array area of 5800-square meters for the thin-membrane design concept with CuInS2 thin-film cells. Power processing unit input power for a thin-membrane array design with three-junction, amorphous SiGe solar cells is shown in the graph. Power falls off rapidly inhe first weeks of the mission because of light-induced (Staebler-Wronksi) solar cell losses. During the next 200 days, power decreases steadily as the SEP stage spirals through the proton belts and sustains the bulk of the mission radiation damage. Once the vehicle apogee is above approximately four Earth radii, little additional degradation is incurred. From 400 to 800 days, a 1100-km "parking" orbit is maintained to await the next payload transfer opportunity. This orbit is below the main proton belt, and thus, little radiation dose is accumulated during this time period. During the second LEO-to-HEEPO transfer, power degrades somewhat further, but power requirements are still met. In comparison, the Europa Mapper SEP vehicle power system had a mass of 150 kg and a thin membrane array area of 100 square meters.

Kerslake, Thomas W.↗

A high-temperature wideband pressure transducer

A condenser microphone AM carrier system, which has been developed to measure pressure fluctuations at elevated temperatures, consists of the following components: a condenser microphone designed for operation at elevated temperatures; existing carrier electronics developed under two previous research grants but adapted to meet present requirements; a 6 m cable operating as a half-wavelength transmission line between the microphone and carrier electronics; and a voltage-controlled oscillator used in a feedback loop for automatic tuning control. Both theoretical and practical aspects of the development program are considered. The three predominant effects of temperature changes are changes in the membrane-backplate gap, membrane tension, and air viscosity. The microphone is designed so that changes in gap and membrane tension tend to have compensating effects upon the microphone sensitivity.

Zuckerwar, A. J.↗

On the suppression of zero energy deformation modes

Based on the Hellinger-Reissner principle and the deformation energy due to assumed stresses and displacements, the problem of the kinematic deformation modes in assumed stress hybrid/mixed finite elements has been examined. Basic schemes are developed for the choice of assumed stress terms that will suppress all kinematic deformation modes. Quadrilateral membrane and axisymmetric elements, and three-dimensional hexahedral elements, are used to illustrate the suggested procedure.

Pian, T. H. H.↗

Thin-film power-density meter for millimeter wavelengths

A quasi-optical power density meter for millimeter and submillimeter wavelengths has been developed. The device is a 2-cm2 thin-film bismuth bolometer deposited on a mylar membrane. The resistance responsivity is 150 Ohms/W, and the time constant is 1 min. The meter is calibrated at DC. The bolometer is much thinner than a wavelength, and can thus be modeled as a lumped resistance in a transmission-line equivalent circuit. The absorption coefficient is 0.5 for 189-Ohms/square film. The power-density meter has been used to measure absolute power densities for millimeter-wave antenna efficiency measurements. Absolute power densities of 0.5 mW/sq cm have been measured to an estimated accuracy of 5 percent.

Lee, Karen A.↗

Changes in Arabidopsis leaf ultrastructure, chlorophyll and carbohydrate content during spaceflight depend on ventilation

Leaf structure and function under spaceflight conditions have received little study despite their important implications for biological life support systems using plants. Previous reports described disruption of the membrane apparatus for photosynthesis and a general decrease in carbohydrate content in foliage. During a series of three short-duration experiments (Chromex-03, -04, -05) on the US space shuttle (STS-54, STS-51, STS-68), we examined Arabidopsis thaliana leaves. The plants were at the rosette stage at the time of loading onto the space shuttle, and received the same light, temperature, carbon dioxide and humidity regimes in the orbiter as in ground controls. The experiments differed according to the regime provided in the headspace around the plants: this was either sealed (on mission STS-54); sealed with high levels of carbon dioxide (on mission STS-51) or vented to the cabin air through a filtration system (on mission STS-68). Immediately post-flight, leaf materials were fixed for microscopy or frozen in liquid nitrogen for subsequent analyses of chlorophyll and foliar carbohydrates. At the ultrastructural level, no aberrations in membrane structure were observed in any of the experiments. When air-flow was provided, plastids developed large starch grains in both spaceflight and ground controls. In the experiments with sealed chambers, spaceflight plants differed from ground controls with regard to measured concentrations of carbohydrate and chlorophyll, but the addition of airflow eliminated these differences. The results point to the crucial importance of consideration of the foliage microenvironment when spaceflight effects on leaf structure and metabolism are studied.

STS-51 Shuttle Project↗

Liquid Metering Centrifuge Sticks (LMCS): A Centrifugal Approach to Metering Known Sample Volumes for Colorimetric Solid Phase Extraction (C-SPE)

Phase separation is one of the most significant obstacles encountered during the development of analytical methods for water quality monitoring in spacecraft environments. Removing air bubbles from water samples prior to analysis is a routine task on earth; however, in the absence of gravity, this routine task becomes extremely difficult. This paper details the development and initial ground testing of liquid metering centrifuge sticks (LMCS), devices designed to collect and meter a known volume of bubble-free water in microgravity. The LMCS uses centrifugal force to eliminate entrapped air and reproducibly meter liquid sample volumes for analysis with Colorimetric Solid Phase Extraction (C-SPE). C-SPE is a sorption-spectrophotometric platform that is being developed as a potential spacecraft water quality monitoring system. C-SPE utilizes solid phase extraction membranes impregnated with analyte-specific colorimetric reagents to concentrate and complex target analytes in spacecraft water samples. The mass of analyte extracted from the water sample is determined using diffuse reflectance (DR) data collected from the membrane surface and an analyte-specific calibration curve. The analyte concentration can then be calculated from the mass of extracted analyte and the volume of the sample analyzed. Previous flight experiments conducted in microgravity conditions aboard the NASA KC-135 aircraft demonstrated that the inability to collect and meter a known volume of water using a syringe was a limiting factor in the accuracy of C-SPE measurements. Herein, results obtained from ground based C-SPE experiments using ionic silver as a test analyte and either the LMCS or syringes for sample metering are compared to evaluate the performance of the LMCS. These results indicate very good agreement between the two sample metering methods and clearly illustrate the potential of utilizing centrifugal forces to achieve phase separation and metering of water samples in microgravity.

Gazda, Daniel B.↗