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At least 433 records · Page 24

Bioaerosols as indicators of central Arctic ice nucleating particle sources

The Arctic is warming at a rapid rate, with implications for microbial communities as the ecosystems change. Some microbes and biogenic materials can affect the persistence of long-lived mixed-phase clouds by serving as ice nucleating particles (INPs). The presence of INPs modulates the cloud phase, and long-term measurements are important to elucidate their seasonal sources and to predict future change. The Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC) expedition in 2019–2020 provided the first year-long measurements of bioaerosols and INPs in the central Arctic, with 3 d filters for amplicon sequencing and cumulative INP concentrations from −5 to −30 °C. Here, we investigated the INP seasonal cycle and its relation to the seasonal cycle of bacteria and eukaryotes. INPs were greatly elevated and compositionally similar in summer, aligning with a greater prevalence of local bioaerosol sources, but, despite this, a diverse mixture of sources (marine and terrestrial) was present all times. A common broader Arctic INP population is hypothesized for much of the year by comparable coincident data collected in Svalbard and a sensitivity of both the INPs and bioaerosols to large-scale events.

Barry, Kevin R. [Colorado State Univ., Fort Collin↗

CAZyme domain architectures suggest fine-scale functional differentiation among anaerobic fungi and bacteria during lignocellulose conversion to volatile fatty acids

Anaerobic fermentation with microbial communities (microbiomes) is an emerging platform for conversion of lignocellulosic biomass to biofuels and bioproducts. The process relies on diverse anaerobic microbes that interact to deconstruct and convert lignocellulosic biomass into a range of products, such as volatile fatty acids (VFAs), which can be achieved by arresting methanogenesis during fermentation. However, defining the distinct functional roles played by various fungi and bacteria during anaerobic biodegradation remains poorly understood. Here, we performed parallel enrichment experiments from cow faeces, goat faeces, and anaerobic digester sludge, selecting for fungal or bacterial dominated communities that convert sorghum biomass into VFAs. Subsequently we reconstructed metabolic networks across these enrichments based on recovered bacterial metagenome-assembled genomes (MAGs) and fungal isolate genomes and profiled their metabolic activity using metatranscriptomics to identify potential functional niches. Our findings implicate diverse bacteria affiliated with the Bacteroidales and Lachnospiraceae in the direct conversion of lignocellulosic biomass to propionate and butyrate, respectively, whereas Neocallimastix-dominated fungal enrichments converted lignocellulose to lactate, acetate and formate. Analysis of carbohydrate-active enzymes (CAZymes) revealed fine-scale differences between microbes that expressed unique multi-functional enzymes linking two or more CAZymes together with distinct carbohydrate binding motifs, implicating lignocellulose structure as a key driver of selection and niche differentiation. Most of these multi-functional enzymes localized complementary degradation functions together, likely conferring synergistic degradation effects within and between microbiome members. We anticipate that these findings will help inform efforts to develop synthetic microbiomes with tailored functionality for low-cost conversion of lignocellulosic biomass to fuels and bio-based chemicals.

Lawson, Christopher E [University of Toronto;]↗

UFMP and CAW Bioreactor Metagenome

CSTR anaerobic bioreactors fed UltraFiltered Milk Permeate (UFMP) and Cottage cheese Acid Whey (CAW) were inoculated with acid sludge microbial communities and monitored overtime for lactic acid production. Samples were taken for metagenomics analyses.

bioreactor metagenome↗

LACSD BNR Pilot Plant Metagenomes

In this study, we aimed to evaluate Biological Nutrient Removal (BNR) and investigate microbial community changes when the DO is reduced in the aerated portions of wastewater treatment trains. We present a dataset of metagenomes obtained from activated sludge collected from the Los Angeles Sanitary District BNR pilot plant at the beginning of operation, when the DO was high, and at the end of operation, when the DO was low.

metagenome↗

Literature review of human microbes' interaction with plants

Human carried microorganisms, which cannot practically be excluded from human supporting agricultural systems of extra terrestrial stations, are considered. These microorganisms damage the plants on which the people depend for oxygen and food. The inclusion of carefully screened or constructed, but more or less normal, phylloplane and rhizosphere microbial communities is studied.

Maguire, B., Jr.↗

Planetary biology and microbial ecology. Biochemistry of carbon and early life

Experiments made with cyanobacteria, phototrophic bacteria, and methanogenic bacteria are detailed. Significant carbon isotope fractionation data is included. Taken from well documented extant microbial communities, this data provides a basis of comparison for isotope fractionation values measured in Archean and Proterozoic (preCambrian) rocks. Media, methods, and techniques used to acquire data are also described.

Margulis, L.↗

Protocyanobacteria: Oxygenic and Anoxygenic photosynthesis in mat-forming bacteria

The oldest record of life is preserved in prePhanerozoic stromatolites dated 3500 million years old and is most likely of filamentous mat-forming cyanobacteria. The sedimentary records of cyanobacterial mats in stromatolites are the most abundant record of life throughout the prePhanerozoic. Stromatolites persisted into the Phanerozoic Eon, yet they become much less pronounced relative to earlier ones. The abundance and persistence of cyanobacterial mats throughout most of geological time point to the evolutionary success of these kinds of microbial communities and their possible role in the evolution of the earth and atmosphere.

Cohen, Y.↗

Glutathione in cyanobacteria

The effects of light and O2 on glutathione production were determined. Results of light and dark studies under normal and reduced oxygen tensions were compared to determine the effect of reduction in oxygen tension on glutathione levels. The growth rate of Anacystis nidulans and concurrent production of glutathione is presented. The generation of time of Anacystis nidulans was approximately 12 hours. Results of light and dark incubation of Aphanothece halophytica dominated planktonic microbial community from Pond 4 and Anacystis nidulans under high and low oxygen tension is also presented. It appears that light grown Anacystis nidulans cells have equal amounts of glutathione while dark grown cells produce more glutathione in the presence of increased O2.

Bermudes, D.↗

Submarine hydrothermal environments as sites for the origin and evolution of life

That life formed and evolved in hydrothermal environments is proposed. This hypothesis is plausible in terms of the tectonic, paleontological, and degassing history of the Earth. Submarine hydrothermal vents are the only contemporary geological environment which may truly be called primeval and which today continue to be a major source of gases and dissolved elements to the ocean. The microbial assemblages in present day hydrothermal systems therefore could be living analogues of the earliest microbial communities to develop on Earth. The evidence for the hypothesis is reviewed.

Hoffman, S.↗

Recent progress in Precambrian paleobiology

Ongoing studies at UCLA include the following: (1) investigations in Archean and Proterozoic sequences of various locations; (2) laboratory and field studies of modern microbial biocoenoses (analogues of Precambrian microbial communities) especially those at Laguna Mormona, Baja California, Mexico; (3) development of new laboratory techniques for the separation and concentration of minute cellularly preserved fossils for isotopic and organic geochemical analyses; and (4) assembly of a computerized database for assessment of the timing and nature of major events occurring during Precambrian biotic evolution, and of the potential applicability of ancient microbiotas to problems of global biostratigraphy and biogeography.

Schopf, J. W.↗

Microfossils in the Antarctic cold desert: Possible implications for Mars

In the Ross Desert of Antarctica, the principal life form is the cryptoendolithic microbial community in the near-surface layers of porous sandstone rocks. Biological, geological, and climatic factors interact in a complex and precarious balance, making life possible in an otherwise hostile environment. Once this balance is tipped, fossilization sets in. In the reverse case, new colonization of the rock surface may be initiated. As a result, fossilization is contemporary with modern life and both may be simultaneously present in a mosaic pattern. Also, different stages of fossilization are present. The process of fossilization takes place in a nonaquatic environment. If primitive life ever appeared on Mars, it is possible that with increasing aridity, life withdrew into an endolithic niche similar to that in the Antarctic desert. Fossilization in a nonaquatic environment may have set in with the result that traces of past life could be preserved. If such was the case, the study of the fossilization process in Antarctica may hold useful information for the analysis of Martian samples for microfossils.

Friedmann, E. I.↗

Cryoconite holes on glaciers

Cryoconite holes are water-filled depressions on the surface of glaciers. They contain microbial communities and may contribute to glacial wastage and biological colonization of ice-free areas.

Wharton, R. A., Jr.↗

Seasonal cycling of sulfur and iron in porewaters of a Delaware salt marsh

An extensive pore water data set has been gathered in the Great Marsh, Delaware over various seasons, salinities, and tides. The data all point to a complimentary redox cycle for sulfur and iron which operates seasonally and tidally. Surface oxidizing conditions prevail in summer, with more reducing conditions at depth during the winter. During the spring tides which flood the marsh, pyrite oxidation occurs releasing excess dissolved iron (II) and sulfate to the porewaters, and precipitating authigenic solid iron phases. The redox conditions in the porewaters of the upper zone during the summer is poised between mildly oxidizing and mildly reducing conditions as shown by pE calculations. This redox environment and intermediate iron-sulfur redox species may be important for the stimulation of plant growth (photosynthesis) and sustenance of a viable microbial community (heterotrophy and chemoautropy).

Luther, George W., III↗

Life on Mars - How it disappeared (if it was ever there)

Information available on Mars chemistry suggest that conditions on early Mars may have been suitable for life. This paper examines the possible events that led to the disappearance of life, assuming it existed, from the surface of Mars. The sequence of events leading to life extinction on early Mars assumes the following steps: (1) a decrease of temperature and humidity levels, leading to a selection of microorganisms for tolerance of low temperatures and arid conditions; (2) further deterioration of environment leading to withdrawal of cold-adapted organisms to protected niches under the surface; (3) further cooling producing heavy stresses in these organisms; and (4) further deterioration of the environment resulting in extinction. This sequence of events is considered parallel events documented for the microbial community in the Ross Desert of Antarctica, where TEM examinations of the material detected progressive stages of cell damage and death.

Friedmann, E. Imre↗

The implantation of life on Mars - Feasibility and motivation

Scientific concepts are reviewed regarding the potential formation and development of a life-bearing environment on Mars, and a potential ecopoiesis scenario is given. The development of the earth's biosphere is defined, and the major assumptions related to the formation of Martian life are listed. Three basic phases are described for the life-implantation concept which include determining whether sufficient quantities of volatiles are available, engineering the warming of the planet, and implanting microbial communities if necessary. Warming the planet theoretically releases liquid H2O and produces a thick CO2 atmosphere, and the implantation of biological communities is only necessary if no indigenous microbes emerge. It is concluded that a feasibility study is required to assess the possibilities of implanting life on Mars more concretely.

Haynes, Robert H.↗

On the possibility of chemosynthetic ecosystems in subsurface habitats on Mars

Recent discoveries of nonphotosynthetic microbial ecosystems on earth have prompted the present reexamination of the prospects for microbial life on Mars, where well-protected subsurface niches associated with hydrothermal activity could have furnished a refuge after surface conditions became inhospitable. It is noted that extensive geological features attest to widespread ground ice-volcanism interactions. Attention is given to the possibility of anaerobic systems employing CO2 as the primary source of carbon, and liquid water furnished by melted subsurface permafrost. Gases from deep volcanic activity could effect reduction, thereby establishing a chemolithoautotrophic basis for a methanogenic or acetogenic and sulfur-based ecology microbial community.

Boston, Penelope J.↗

Temperature Affects Fatty Acids In Methylococcus Capsulatus

According to report, temperature of growth of thermotolerant, methane-oxidizing bacterium Methylococcus capsulatus (Bath) affects both proportion of monounsaturated fatty acids and cis/trans ratio of these acids in cell membrane. Because suboptimum growth temperature is potential stress factor, it may be possible to use such cis/trans ratios as indices of stresses upon methane-oxidizing microbial communities. Research in microbiology of methanotrophs increasing because of possible commercial exploitation of these organisms as biocatalysts or as sources of useful polymers; knowledge of effect of temperature on ability of methanotrophs to utilize methane useful in optimization of conditions of growth.

Jahnke, Linda L.↗

A 'crytic' microbial mat: A new model ecosystem for extant life on Mars

If life were present on Mars today, it would face potentially lethal environmental conditions such as a lack of water, frigid temperatures, ultraviolet radiation, and soil oxidants. In addition, the Viking missions did not detect near-surface organic carbon available for assimilation. Autotrophic organisms that lived under a protective layer of sand or gravel would be able to circumvent the ultraviolet radiation and lack of fixed carbon. Two terrestrial photosynthetic near-surface microbial communities have been identified, one in the inter- and supertidal of Laguna Ojo de Liebere (Baja California Sur, Mexico) and one in the acidic gravel near several small geysers in Yellowstone National Park (Wyoming, U.S.A.). Both communities have been studied with respect to their ability to fix carbon under different conditions, including elevated levels of inorganic carbon. Although these sand communities have not been exposed to the entire suite of Martian environmental conditions simultaneously, such communities can provide a useful model ecosystem for a potential extant Martian biota.

Rothschild, L. J.↗