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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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416 records · Page 24

The Nature of Standing Plasma Waves Following Fast Breakdown in Thunderstorms

Narrow bipolar events (NBEs) are produced by large-scale systems of streamer corona discharges that occur inside thunderstorms and radiate intensely at very-high frequencies. Here we examine 14 high-power NBEs recorded in Florida which are followed by a lasting oscillatory signal. We isolate the dominant frequencies of the oscillations using empirical mode decomposition methods, and cross-reference this information with the radiator size inferred from broadband interferometry. We conclude that the oscillations are evidence of a standing electromagnetic wave in the plasma cavity created by the preceding streamer system, that is, the cavity's fundamental mode with wavelength equal to twice the radiator length. Contrasting the dispersive and attenuation properties of these waves across several different events allows us to conclude that the cavity is filled with ions resulting in an average conductivity of 0.01–0.1 mS/m, which are left behind after the streamers decay via electron attachment to oxygen molecules.

58 GEOSCIENCES↗

Comprehensive characterization of N- and O- glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2

The emergence of the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has created the need for development of new therapeutic strategies. Understanding the mode of viral attachment, entry and replication has become a key aspect of such interventions. The coronavirus surface features a trimeric spike (S) protein that is essential for viral attachment, entry and membrane fusion. The S protein of SARS-CoV-2 binds to human angiotensin converting enzyme 2 (hACE2) for entry. Herein, we describe glycomic and glycoproteomic analysis of hACE2 expressed in HEK293 cells. We observed high glycan occupancy (73.2 to 100%) at all seven possible N-glycosylation sites and surprisingly detected one novel O-glycosylation site. To deduce the detailed structure of glycan epitopes on hACE2 that may be involved in viral binding, we have characterized the terminal sialic acid linkages, the presence of bisecting GlcNAc and the pattern of N-glycan fucosylation. We have conducted extensive manual interpretation of each glycopeptide and glycan spectrum, in addition to using bioinformatics tools to validate the hACE2 glycosylation. Our elucidation of the site-specific glycosylation and its terminal orientations on the hACE2 receptor, along with the modeling of hACE2 glycosylation sites can aid in understanding the intriguing virus-receptor interactions and assist in the development of novel therapeutics to prevent viral entry. Here, the relevance of studying the role of ACE2 is further increased due to some recent reports about the varying ACE2 dependent complications with regard to age, sex, race and pre-existing conditions of COVID-19 patients.

59 BASIC BIOLOGICAL SCIENCES↗