Results for “Attachments” — Page 25

NASA NTRS2011

Low Fluid Shear Culture of Staphylococcus Aureus Represses hfq Expression and Induces an Attachment-Independent Biofilm Phenotype

Background: The opportunistic pathogen, Staphylococcus aureus, experiences fluctuations in fluid shear during infection and colonization of a human host. Colonization frequently occurs at mucus membrane sites such as in the gastrointestinal tract where the bacterium may experience low levels of fluid shear. The response of S. aureus to low fluid shear remains unclear. Methods: S. aureus was cultured to stationary phase using Rotating-Wall Vessel (RWV) bioreactors which produce a physiologically relevant low fluid shear environment. The bacterial aggregates that developed in the RWV were evaluated by electron microscopy as well as for antibiotic resistance and other virulence-associated stressors. Genetic expression profiles for the low-shear cultured S. aureus were determined by microarray analysis and quantitative real-time PCR. Results: Planktonic S. aureus cultures in the low-shear environment formed aggregates completely encased in high amounts of extracellular polymeric substances. In addition, these aggregates demonstrated increased antibiotic resistance indicating attachment-independent biofilm formation. Carotenoid production in the low-shear cultured S. aureus was significantly decreased, and these cultures displayed an increased susceptibility to oxidative stress and killing by whole blood. The hfq gene, associated with low-shear growth in Gram negative organisms, was also found to be down-regulated in S. aureus. Conclusions: Collectively, this data suggests that S. aureus decreases virulence characteristics in favor of a biofilm-dwelling colonization phenotype in response to a low fluid shear environment. Furthermore, the identification of an Hfq response to low-shear culture in S. aureus, in addition to the previously reported responses in Gram negative organisms, strongly suggests an evolutionarily conserved response to mechanical stimuli among structurally diverse prokaryotes.

Ott, C. Mark↗

NASA NTRS2005

Magnetically Attached Multifunction Maintenance Rover

A versatile mobile telerobot, denoted the magnetically attached multifunction maintenance rover (MAGMER), has been proposed for use in the inspection and maintenance of the surfaces of ships, tanks containing petrochemicals, and other large ferromagnetic structures. As its name suggests, this robot would utilize magnetic attraction to adhere to a structure. As it moved along the surface of the structure, the MAGMER would perform tasks that could include close-up visual inspection by use of video cameras, various sensors, and/or removal of paint by water-jet blasting, laser heating, or induction heating. The water-jet nozzles would be mounted coaxially within compressed-air-powered venturi nozzles that would collect the paint debris dislodged by the jets. The MAGMER would be deployed, powered, and controlled from a truck, to which it would be connected by hoses for water, compressed air, and collection of debris and by cables for electric power and communication (see Figure 1). The operation of the MAGMER on a typical large structure would necessitate the use of long cables and hoses, which can be heavy. To reduce the load of the hoses and cables on the MAGMER and thereby ensure its ability to adhere to vertical and overhanging surfaces, the hoses and cables would be paid out through telescopic booms that would be parts of a MAGMER support system. The MAGMER would move by use of four motorized, steerable wheels, each of which would be mounted in an assembly that would include permanent magnets and four pole pieces (see Figure 2). The wheels would protrude from between the pole pieces by only about 3 mm, so that the gap between the pole pieces and the ferromagnetic surface would be just large enough to permit motion along the surface but not so large as to reduce the magnetic attraction excessively. In addition to the wheel assemblies, the MAGMER would include magnetic adherence enhancement fixtures, which would comprise arrays of permanent magnets and pole pieces that could be adjusted to maximize or minimize the overall attractive magnetic force. Even with a paint thickness of 2 mm, a preliminary design provides a safety factor of 5 in the magnetic force in the upside- down, water-jets-operating condition, in which the total load (including the weight of the MAGMER and cables and the water-jet force) would be about 260 lb (the weight of 118 kg). Optionally, the MAGMER could carry magnetic shielding and/or could be equipped with a demagnetizing module to remove residual magnetism from the structure. The MAGMER would carry four charge-coupled-device cameras for visual inspection, monitoring of operation, navigation, and avoidance of collisions with obstacles. The control system of the MAGMER would include navigation and collision-avoidance subsystems that would utilize surface features as landmarks, in addition to direct images of obstacles.

Bar-Cohen, Yoseph↗

NASA NTRS2003

Multiple-Use Mechanisms for Attachment to Seat Tracks

A Seat Track Attach Mechanism (SAM) is a multiple-use clamping device intended for use in mounting various objects on the standard seat tracks used on the International Space Station (ISS). The basic SAM design could also be adapted to other settings in which seat tracks are available: for example, SAM-like devices could be used as universal aircraft-seat-track mounting clamps. A SAM (see figure) is easily installed by inserting it in a seat track, then actuating a locking lever to clamp the SAM to the track. The SAM includes an over-center locking feature that prevents premature disengagement that could be caused by some inadvertent movements of persons or objects in the vicinity. A SAM can be installed in, or removed from, any position along a seat track, without regard for the locations of the circular access holes. Hence, one or more SAM(s) can be used to mount an object or objects on a track or a pair of tracks in an infinite number of preferred configurations. A SAM can be incorporated into a dual swivel device, so that two of the SAMs can be made to lock onto two side-by-side seat tracks simultaneously, as would be the case in a standard ISS rack bay where two side-by-side racks reside. The main benefit to using two SAMs in a side-by-side arrangement is to provide a coupled load. By picking up load points on two seat tracks, a coupled loading is created, improving the stability and strength since the load is spread to two seat tracks at a short distance.

Fraske, Martin↗

NASA NTRS2014

Rapid Startup and Loading of an Attached Growth, Simultaneous Nitrification/Denitrification Membrane Aerated Bioreactor

The Membrane Aerated Bioreactor (MABR) is an attached‐growth biological system for simultaneous nitrification and denitrification. This design is an innovative approach to common terrestrial wastewater treatments for nitrogen and carbon removal. Implementing a biologically‐based water treatment system for long‐duration human exploration is an attractive, low energy alternative to physiochemical processes. Two obstacles to implementing such a system are (1) the "start‐up" duration from inoculation to steady‐state operations and (2) the amount of surface area needed for the biological activity to occur. The Advanced Water Recovery Systems (AWRS) team at JSC explored these two issues through two tests; a rapid inoculation study and a wastewater loading study. Results from these tests demonstrate that the duration from inoculation to steady state can be reduced to two weeks and that the surface area to volume ratio baseline used in the Alternative Water Processor (AWP) test was higher than what was needed to remove the organic carbon and ammonium from the system.

Meyer, Caitlin↗

NASA NTRS2015

Fast Response, Open-Celled Porous, Shape Memory Effect Actuators with Integrated Attachments

This invention relates to the exploitation of porous foam articles exhibiting the Shape Memory Effect as actuators. Each foam article is composed of a plurality of geometric shapes, such that some geometric shapes can fit snugly into or around rigid mating connectors that attach the Shape Memory foam article intimately into the load path between a static structure and a moveable structure. The foam is open-celled, composed of a plurality of interconnected struts whose mean diameter can vary from approximately 50 to 500 microns. Gases and fluids flowing through the foam transfer heat rapidly with the struts, providing rapid Shape Memory Effect transformations. Embodiments of porous foam articles as torsional actuators and approximately planar structures are disposed. Simple, integral connection systems exploiting the ability to supply large loads to a structure, and that can also supply hot and cold gases and fluids to effect rapid actuation are also disposed.

Jardine, Andrew Peter↗

NASA NTRS2011

Frame Synchronization Without Attached Sync Markers

We describe a method to synchronize codeword frames without making use of attached synchronization markers (ASMs). Instead, the synchronizer identifies the code structure present in the received symbols, by operating the decoder for a handful of iterations at each possible symbol offset and forming an appropriate metric. This method is computationally more complex and doesn't perform as well as frame synchronizers that utilize an ASM; nevertheless, the new synchronizer acquires frame synchronization in about two seconds when using a 600 kbps software decoder, and would take about 15 milliseconds on prototype hardware. It also eliminates the need for the ASMs, which is an attractive feature for short uplink codes whose coding gain would be diminished by the overheard of ASM bits. The lack of ASMs also would simplify clock distribution for the AR4JA low-density parity-check (LDPC) codes and adds a small amount to the coding gain as well (up to 0.2 dB).

Hamkins, Jon↗

NASA NTRS2015

Rapid Start-up and Loading of an Attached Growth, Simultaneous Nitrification/Denitrification Membrane Aerated Bioreactor

Membrane aerated bioreactors (MABR) are attached-growth biological systems used for simultaneous nitrification and denitrification to reclaim water from waste. This design is an innovative approach to common terrestrial wastewater treatments for nitrogen and carbon removal and implementing a biologically-based water treatment system for long-duration human exploration is an attractive, low energy alternative to physiochemical processes. Two obstacles to implementing such a system are (1) the "start-up" duration from inoculation to steady-state operations and (2) the amount of surface area needed for the biological activity to occur. The Advanced Water Recovery Systems (AWRS) team at JSC explored these two issues through two tests; a rapid inoculation study and a wastewater loading study. Results from these tests demonstrate that the duration from inoculation to steady state can be reduced to under two weeks, and that despite low ammonium removal rates, the MABRs are oversized.

Meyer, Caitlin E.↗

NASA NTRS2015

Modular Apparatus and Method for Attaching Multiple Devices

A modular apparatus for attaching sensors and electronics is disclosed. The modular apparatus includes a square recess including a plurality of cavities and a reference cavity such that a pressure sensor can be connected to the modular apparatus. The modular apparatus also includes at least one voltage input hole and at least one voltage output hole operably connected to each of the plurality of cavities such that voltage can be applied to the pressure sensor and received from the pressure sensor.

Okojie, Robert S↗

NASA NTRS2018

Modular Apparatus and Method for Attaching Multiple Devices

A modular apparatus for attaching sensors and electronics is disclosed. The modular apparatus includes a square recess including a plurality of cavities and a reference cavity such that a pressure sensor can be connected to the modular apparatus. The modular apparatus also includes at least one voltage input hole and at least one voltage output hole operably connected to each of the plurality of cavities such that voltage can be applied to the pressure sensor and received from the pressure sensor.

Okojie, Robert S.↗

NASA NTRSDate not supplied

Operating and Maintaining the JWST Cleanroom attached to JSC's Chamber A during preparations for the OTIS Cryogenic Thermal Vacuum Test

In order to maintain cleanliness during preparations for JWST’s OTIS (Optical Telescope Element-Integrated Science Instrument Module) Cryogenic Thermal Vacuum Test, a cleanroom was built that attached directly to the 60-year-old Chamber A. The cleanroom and chamber were outfitted with independent environmental control systems each providing ISO Class 71 air cleanliness. To maintain balanced, positive pressure in both the cleanroom and chamber volumes, a special control protocol was developed and successfully implemented. Dual back-up environmental control units (one each for the chamber and cleanroom) were installed just outside the building to provide environmental control redundancy due to a single source chilled water supply and weather threats. In addition, lack of a dedicated cleanroom airlock facilitating clean ingress and egress made it necessary to perform additional cleaning and packaging, as well as augment the uncontrolled truck lock space with small clean tents for pre-cleaning. Special procedures were developed to allow ingress of extra-large support equipment required for load testing of the cleanroom crane, installation of optical equipment in Chamber A and accommodation of the OTIS shipping container. A thorough bake-out and cleaning of Chamber A was also necessary to reduce volatiles from the shroud’s black thermal paint and to reduce particle fallout. Acrylic adhesive fracture discovered during early cryo-testing represented a significant challenge that was successfully mitigated prior to OTIS testing. A dedicated team of Contamination Control (CC) Technicians was specifically trained to clean support equipment and screen materials entering the cleanroom and chamber to ensure cleanliness and vacuum compatibility.

Optics↗

NASA NTRSDate not supplied

Operating and Maintaining the JWST Cleanroom attached to JSC's Chamber A during preparations for the OTIS Cryogenic Thermal Vacuum Test

In order to maintain cleanliness during preparations for JWST’s OTIS (Optical Telescope Element-Integrated Science Instrument Module) Cryogenic Thermal Vacuum Test, a cleanroom was built that attached directly to the 60-year-old Chamber A. The cleanroom and chamber were outfitted with independent environmental control systems each providing ISO Class 71 air cleanliness. To maintain balanced, positive pressure in both the cleanroom and chamber volumes, a special control protocol was developed and successfully implemented. Dual back-up environmental control units (one each for the chamber and cleanroom) were installed just outside the building to provide environmental control redundancy due to a single source chilled water supply and weather threats. In addition, lack of a dedicated cleanroom airlock facilitating clean ingress and egress made it necessary to perform additional cleaning and packaging, as well as augment the uncontrolled truck lock space with small clean tents for pre-cleaning. Special procedures were developed to allow ingress of extra-large support equipment required for load testing of the cleanroom crane, installation of optical equipment in Chamber A and accommodation of the OTIS shipping container. A thorough bake-out and cleaning of Chamber A was also necessary to reduce volatiles from the shroud’s black thermal paint and to reduce particle fallout. Acrylic adhesive fracture discovered during early cryo-testing represented a significant challenge that was successfully mitigated prior to OTIS testing. A dedicated team of Contamination Control (CC) Technicians was specifically trained to clean support equipment and screen materials entering the cleanroom and chamber to ensure cleanliness and vacuum compatibility.

James Webb Space Telescope↗

DOE OSTI2021

Methods for attaching transmitters to animals

Methods for attaching a radio frequency (RF) transmitter to an animal are provided. The methods can include providing an RF transmitter and providing an injection device having a needle of gauge of 9 or smaller; providing the RF transmitter into the injection device; and providing the RF transmitter through the 9 gauge or smaller needle and into the animal.

Deng, Z. Daniel↗

DOE OSTI2022

Nevada National Security Site Environmental Report 2021 with 2021 Environmental Report Attachment A: Site Description

This Nevada National Security Site Environmental Report (NNSSER) summarizes actions taken in 2021 to protect the environment and the public while achieving the NNSA/NFO mission goals. It is prepared for the public and our stakeholders in hopes that it is readily understandable and usable. It is a key component in our efforts to keep the public informed of environmental conditions at the NNSS and its support facilities in Las Vegas, Nevada. The accompanying Attachment A expands on the general description of the Nevada National Security Site (NNSS) presented in the Introduction to the Nevada National Security Site Environmental Report 2021. Included are subsections that summarize the site’s geological, hydrological, climatological, and ecological settings and the cultural resources of the NNSS.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2022

Methods for attaching transmitters to animals

Methods for attaching a radio frequency (RF) transmitter to an animal are provided. The methods can include providing an RF transmitter and providing an injection device having a needle of gauge of 9 or smaller; providing the RF transmitter into the injection device; and providing the RF transmitter through the 9 gauge or smaller needle and into the animal.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Torsionally flexible attachment system and method

A flexible attachment assembly is described having a bellows capable of absorbing torsion having a first end; a second end opposite to the first end; and a plurality of tubes, each of which is hollow and wound around a longitudinal axis while extending from the first end to the second end, where the plurality of tubes are communicated with each other all along the longitudinal length of the bellows so as to form a bore between the first end and the second end. Also provided is a bellows assembly having at least two sections, each section having a plurality of tubes, each of which is hollow and wound around a longitudinal axis and thus form a bore, where the plurality of sections are connected with each other so that their bores are communicated.

Schmidt, Oliver A.↗

DOE OSTI2024

Nevada National Security Site Environmental Report 2023, with Attachment A Site Description and Summary Report

This Nevada National Security Site Environmental Report (NNSSER) summarizes actions taken in 2023 to protect the environment and the public while achieving the NNSA/NFO mission goals. It is prepared for the public and our stakeholders in hopes that it is readily understandable and usable. It is a key component in our efforts to keep the public informed of environmental conditions at the NNSS and its support facilities in Las Vegas, Nevada. The accompanying Attachment A expands on the general description of the Nevada National Security Site (NNSS) presented in the Introduction to the Nevada National Security Site Environmental Report 2023. Included are subsections that summarize the site’s geological, hydrological, climatological, and ecological settings and the cultural resources of the NNSS. The supplemental Summary report provides an abbreviated version of the full report.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2024

Nevada National Security Site Environmental Report 2023; Attachment A Site Description

This Nevada National Security Site Environmental Report (NNSSER) summarizes actions taken in 2023 to protect the environment and the public while achieving the NNSA/NFO mission goals. It is prepared for the public and our stakeholders in hopes that it is readily understandable and usable. It is a key component in our efforts to keep the public informed of environmental conditions at the NNSS and its support facilities in Las Vegas, Nevada. The accompanying Attachment A expands on the general description of the Nevada National Security Site (NNSS) presented in the Introduction to the Nevada National Security Site Environmental Report 2023. Included are subsections that summarize the site’s geological, hydrological, climatological, and ecological settings and the cultural resources of the NNSS. The supplemental Summary report provides an abbreviated version of the full report.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2025

Nevada National Security Site Environmental Report 2024 with Attachment A Site Description

The Nevada National Security Site Environmental Report 2024 Attachment A report expands on the general description of the Nevada National Security Site (NNSS) presented in the Introduction to the Nevada National Security Site Environmental Report 2024. Included are subsections that summarize the site’s geological, hydrological, climatological, and ecological settings and the cultural resources of the NNSS.

54 ENVIRONMENTAL SCIENCES↗