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NASA NTRS2015

Climate Data Initiative: A Geocuration Effort to Support Climate Resilience

Curation is traditionally defined as the process of collecting and organizing information around a common subject matter or a topic of interest and typically occurs in museums, art galleries, and libraries. The task of organizing data around specific topics or themes is a vibrant and growing effort in the biological sciences but to date this effort has not been actively pursued in the Earth sciences. In this paper, we introduce the concept of geocuration and define it as the act of searching, selecting, and synthesizing Earth science data/metadata and information from across disciplines and repositories into a single, cohesive, and useful compendium We present the Climate Data Initiative (CDI) project as an exemplar example. The CDI project is a systematic effort to manually curate and share openly available climate data from various federal agencies. CDI is a broad multi-agency effort of the U.S. government and seeks to leverage the extensive existing federal climate-relevant data to stimulate innovation and private-sector entrepreneurship to support national climate-change preparedness. We describe the geocuration process used in CDI project, lessons learned, and suggestions to improve similar geocuration efforts in the future.

virtual collections↗

NASA NTRS2016

Making Metadata Better with CMR and MMT

Ensuring complete, consistent and high quality metadata is a challenge for metadata providers and curators. The CMR and MMT systems provide providers and curators options to build in metadata quality from the start and also assess and improve the quality of already existing metadata.

Quality↗

NASA NTRS2016

Extending the Reach of IGSN Beyond Earth: Implementing IGSN Registration to Link Nasa's Apollo Lunar Samples and Their Data

The rock and soil samples returned from the Apollo missions from 1969-72 have supported 46 years of research leading to advances in our understanding of the formation and evolution of the inner Solar System. NASA has been engaged in several initiatives that aim to restore, digitize, and make available to the public existing published and unpublished research data for the Apollo samples. One of these initiatives is a collaboration with IEDA (Interdisciplinary Earth Data Alliance) to develop MoonDB, a lunar geochemical database modeled after PetDB (Petrological Database of the Ocean Floor). In support of this initiative, NASA has adopted the use of IGSN (International Geo Sample Number) to generate persistent, unique identifiers for lunar samples that scientists can use when publishing research data. To facilitate the IGSN registration of the original 2,200 samples and over 120,000 subdivided samples, NASA has developed an application that retrieves sample metadata from the Lunar Curation Database and uses the SESAR API to automate the generation of IGSNs and registration of samples into SESAR (System for Earth Sample Registration). This presentation will describe the work done by NASA to map existing sample metadata to the IGSN metadata and integrate the IGSN registration process into the sample curation workflow, the lessons learned from this effort, and how this work can be extended in the future to help deal with the registration of large numbers of samples.

Todd, Nancy S.↗

NASA NTRS2016

Let Our Powers Combine! Harnessing NASA's Earth Observatory Natural Event Tracker (EONET) in Worldview

Constellations of NASA Earth Observing System (EOS) satellites orbit the earth to collect images and data about the planet in near real-time. Within hours of satellite overpass, you can discover where the latest wildfires, severe storms, volcanic eruptions, and dust and haze events are occurring using NASA's Worldview web application. By harnessing a repository of curated natural event metadata from NASA Earth Observatory's Natural Event Tracker (EONET), Worldview has moved natural event discovery to the forefront and allows users to select events-of-interest from a curated list, zooms to the area, and adds the most relevant imagery layers for that type of natural event. This poster will highlight NASA Worldviews new natural event feed functionality.

earth science↗

NASA NTRS2017

The NASA Ames Life Sciences Data Archive: Biobanking for the Final Frontier

The NASA Ames Institutional Scientific Collection involves the Ames Life Sciences Data Archive (ALSDA) and a biospecimen repository, which are responsible for archiving information and non-human biospecimens collected from spaceflight and matching ground control experiments. The ALSDA also manages a biospecimen sharing program, performs curation and long-term storage operations, and facilitates distribution of biospecimens for research purposes via a public website (https:lsda.jsc.nasa.gov). As part of our best practices, a tissue viability testing plan has been developed for the repository, which will assess the quality of samples subjected to long-term storage. We expect that the test results will confirm usability of the samples, enable broader science community interest, and verify operational efficiency of the archives. This work will also support NASA open science initiatives and guides development of NASA directives and policy for curation of biological collections.

Biobank↗

NASA NTRS2017

The NASA Ames Research Center Institutional Scientific Collection: History, Best Practices and Scientific Opportunities

The NASA Ames Life Sciences Institutional Scientific Collection (ISC), which is composed of the Ames Life Sciences Data Archive (ALSDA) and the Biospecimen Storage Facility (BSF), is managed by the Space Biosciences Division and has been operational since 1993. The ALSDA is responsible for archiving information and animal biospecimens collected from life science spaceflight experiments and matching ground control experiments. Both fixed and frozen spaceflight and ground tissues are stored in the BSF within the ISC. The ALSDA also manages a Biospecimen Sharing Program, performs curation and long-term storage operations, and makes biospecimens available to the scientific community for research purposes via the Life Science Data Archive public website (https:lsda.jsc.nasa.gov). As part of our best practices, a viability testing plan has been developed for the ISC, which will assess the quality of archived samples. We expect that results from the viability testing will catalyze sample use, enable broader science community interest, and improve operational efficiency of the ISC. The current viability test plan focuses on generating disposition recommendations and is based on using ribonucleic acid (RNA) integrity number (RIN) scores as a criteria for measurement of biospecimen viablity for downstream functional analysis. The plan includes (1) sorting and identification of candidate samples, (2) conducting a statiscally-based power analysis to generate representaive cohorts from the population of stored biospecimens, (3) completion of RIN analysis on select samples, and (4) development of disposition recommendations based on the RIN scores. Results of this work will also support NASA open science initiatives and guides development of the NASA Scientific Collections Directive (a policy on best practices for curation of biological collections). Our RIN-based methodology for characterizing the quality of tissues stored in the ISC since the 1980s also creates unique scientific opportunities for temporal assessment across historical missions. Support from the NASA Space Biology Program and the NASA Human Research Program is gratefully acknowledged.

ALSDA↗

NASA NTRS2017

GES DISC Datalist Improves Earth Science Data Discoverability

At American Geophysical Union(AGU) 2016 Fall Meeting, Goddard Earth Sciences Data Information Services Center (GES DISC) unveiled a novel way to access data: Datalist. Currently, datalist is a collection of predefined data variables from one or more archived datasets, curated by our subject matter expert (SME). Our science support team has curated a predefined Hurricane Datalist and received very positive feedback from the user community. Datalist uses the same architecture our new website uses and have the same look and feel as other datasets on our web site. and also provides a one-stop shopping for data, metadata, citation, documentation, visualization and other available services. Since the last AGU Meeting, we have further developed a few new datalists corresponding to the Big Earth Data Initiative (BEDI) Societal Benefit Areas and A-Train data. We now have four datalists: Hurricane, Wind Energy, Greenhouse Gas and A-Train. We have also started working with our User Working Group members to create their favorite datalists and working with other DAAC to explore the possibility to include their products in our datalists that may also lead to a future of potential federated (cross-DAAC) datalists. Since our datalist prototype effort was a success, we are planning to make datalist operational. It's extremely important to have a common metadata model to support datalist, this will also be the foundation of federated datalist. We mapped our datalist metadata model to the unpublished UMM(Universal Metadata Model)-Var (Variable) (June version) and found that the UMM-var together with UMM-C (Collection) and possible UMM-S (Service) will meet our basic requirements. For example: Dataset shortname, and version are already specified in UMM-C, variable name, long name, units, dimensions are all specified in UMM-Var. UMM-Var also facilitates Science Keywords to allow tagging at variable level and Characteristics for optional variable characteristics. Measurements is useful for grouping of the variables and Set is promising to define datalist. And finally, the UMM-Service model to specify the available services for the variable will be very beneficial. In summary, UMM-Var, UMM-C and UMM-S are the basis of federated datalist and the development and deployment of datalist will contribute to the evolution of the UMM.

datalist↗

NASA NTRS2018

Public Engagement of Earth Science and Art Using Astronaut Photography

For over fifty years of crewed spaceflight missions, NASA astronauts have taken remarkable photographs of the Earth that offer various perspectives for viewing dynamic Earth processes. The International Space Station (ISS) today provides a unique platform to view and capture imagery of Earth at a variety of viewing angles, seasons, and times of day. Astronaut photos comprise a variable true-color (RGB - Red Green Blue) dataset taken with multiple handheld digital (and historically, film) cameras and lens types that differ from nadir-looking satellite-based remote sensing instruments with fixed temporal and spatial resolutions. The Crew Earth Observations (CEO) Facility within the Earth Science and Remote Sensing Unit at NASA’s Johnson Space Center supports the acquisition, analysis, and curation of astronaut photography of Earth’s surface and atmosphere. CEO’s website, the Gateway to Astronaut Photography of Earth (eol.jsc.nasa.gov), provides free public access to view, search, and download over three million images taken by astronauts from Mercury, Gemini, and Apollo missions to current ISS missions. Handheld images taken by astronauts from the ISS are used for scientific research and public engagement in STEM (Science, Technology, Engineering, Mathematics) fields, particularly earth science. While segments of the public are actively interested in STEM fields, there may be groups who are underexposed to and disinterested in earth science or are unaware of astronaut photography from the ISS. A broader public audience can be introduced to earth science, STEM fields, and ISS research through artistic or abstract photos of the Earth. Wide angle pictures taken of Earth have identifiable landforms and bodies of water, however zoomed in, very high resolution photos without obvious geographic reference can attract new viewing audiences on a purely aesthetic basis. The CEO Facility actively curates a collection of exceptional astronaut photos of Earth as art used to reach a wider population through increased digital products and outreach events. This continuously-updated collection feeds development of freely accessible and high-quality downloadable materials including scientific articles, videos, computer wallpapers, and social media content. These materials are presented with science-based information that augments the artistic qualities of the imagery, and facilitate connection between art-engaged or non-STEM audiences to the ISS research platform and the need for future innovative STEM research.

Meado, Andrea L.↗

NASA NTRS2018

GeneLab: Omics Database for Spaceflight Experiments

Motivation - To curate and organize expensive spaceflight experiments conducted aboard space stations and maximize the scientific return of investment, while democratizing access to vast amounts of spaceflight related omics data generated from several model organisms. Results - The GeneLab Data System (GLDS) is an open access database containing fully coordinated and curated "omics" (genomics, transcriptomics, proteomics, metabolomics) data, detailed metadata and radiation dosimetry for a variety of model organisms. GLDS is supported by an integrated data system allowing federated search across several public bioinformatics repositories. Archived datasets can be queried using full-text search (e.g., keywords, Boolean and wildcards) and results can be sorted in multifactorial manner using assistive filters. GLDS also provides a collaborative platform built on GenomeSpace for sharing files and analyses with collaborators. It currently houses 172 datasets and supports standard guidelines for submission of datasets, MIAME (for microarray), ENCODE Consortium Guidelines (for RNA-seq) and MIAPE Guidelines (for proteomics).

omics↗

NASA NTRS2018

Earth Science Engagement Through Art and Astronaut Photography

For over five decades of manned spaceflight missions, NASA astronauts have taken extraordinary photographs of Earth's surface and dynamic processes. Humans on board the International Space Station (ISS) have a unique platform to perform Earth observations at various viewing angles, seasons, and times of day. Astronaut photos taken from the ISS comprise a true‐color (RGB) dataset taken with multiple handheld digital cameras and lens types (prior to 2004, film cameras were in use). Earth observations through astronaut photography are an important and unique remote sensing method when monitoring natural disasters, urban growth, and environmental changes. While astronaut imagery can be used for earth science research, there is also an artistic aspect to the photography that fascinates a wide global population. A broader public audience can be introduced to earth science through high resolution, Earth art photos taken from the perspective of an astronaut. The Crew Earth Observations (CEO) Facility within the Earth Science and Remote Sensing Unit at NASA's Johnson Space Center supports the acquisition, analysis, and curation of astronaut photography of Earth's surface and atmosphere. CEO's website, the Gateway to Astronaut Photography of Earth (eol.jsc.nasa.gov), provides free public access to view, search, and download over three million images taken by astronauts throughout all of NASA's crewed spaceflight history, with an emphasis on current ISS imagery. The CEO Facility actively curates a digital collection of exceptional Earth art astronaut photos used for public engagement. Our new Downloadable Earth Art page focuses on broad earth science topics including: mountains, water, clouds, agriculture, as well as an "abstract" category. This continuously‐updated collection is comprised of freely accessible and high‐quality downloadable materials, such as single‐ and dual‐screen digital wallpapers. All Earth Art materials are presented with science‐based information that complements the artistic qualities of the imagery, and facilitate connections between general audiences and earth science from the International Space Station.

Meado, Andrea L.↗

NASA NTRS2019

Changing Climate, Changing Data: Exposing Climate Data to New Users Through GeoPlatform.gov’s Resilience Community

Over 700 climate related datasets were curated by subject matter experts into 9 thematic areas as a part of the Climate Data Initiative (CDI). NASA was tasked with maintaining the collection’s data inventory and supporting web pages at data.gov/climate. Today, the Data Curation for Discovery (DCD) team at MSFC continues to support the CDI collection. In order to expose the collection to a new and growing user community, the DCD team has partnered with GeoPlatform.gov to develop the Resilience community. The Resilience community serves as an interactive, topically-focused web portal that further promotes and shares CDI web content, datasets, services, maps, and other tools relevant to global resilience and change. This poster focuses on the team’s efforts to leverage GeoPlatform’s semantic applications to link CDI objects within the platform to improve discoverability. This poster also provides insights as to how this effort may serve as an example for building and expanding future Geoplatform.gov communities..

Sisco, Adam↗

NASA NTRS2019

Lunar Glovebox Balance with Wireless Technology

The most important equipment required for processing lunar samples is a high-quality mass balance for maintaining accurate weight inventory, security, and scientific study. After careful review, a Curation Office memo by Michael Duke in 1978 chose the Mettler PL200 to be used for sample weight measurements inside the gloveboxes (Fig. 3). These commercial off-the-shelf (COTS) balances did not meet the strict accepted material requirements in the Lunar lab. As a result, each balance housing, weighing pan, and wiring was custom retrofitted to meet Lunar Operating Procedure (LOP) 54 requirements [for material construction restrictions]. The original design drawings for the custom housings, readout support stands, and wiring were done by the JSC engineering directorate. The 1977- 1978 schematics, drawings, and files are now housed in the curation Data Center. Per the design specifications, the housing was fabricated from aluminum grade 6061 T6, seamless welds, and anodized per MIL-A-8625 type I, class I. The balance feet were TFE Teflon and any required joints were sealed with Viton A gaskets. The readout display and support stands outside the glovebox were fabricated from 300 series stainless steel with #4 finish and mounted to the glovebox with welded bolts. Wire harnesses that linked the balance with the outside display and power were encapsulated with TFE Teflon and transported through custom Deutsch wire bulk head pass-through systems from inside to outside the glovebox. These Deutsch connectors were custom fabricated with 316L stainless steel bodies, Viton A O-rings, aluminum 6061 with electroless nickel plating, Teflon (replacing the silicone), and gold crimp connectors (no soldering). Many of the Deutsch connectors may have been used in the Apollo program high vacuum complex in building 37 and date to about 1968 to 1970.

Zeigler, Ryan A.↗

NASA NTRS2020

Fungal Exposure to Meteorite Thin Sections: Developing an Experimental to Observe Biogeochemical Changes

The Astromaterials Acquisition & Curation Office maintains collections of meteorite samples collected as part of the Antarctic Search for Meteorites (ANSMET) program. The chief goal of the curation department is to maintain these meteorites in pristine condition. The Astromaterials Research and Exploration Science (ARES) Directorate has implemented a microbial monitoring program for the meteorite collections that has resulted in the isolation of >100 fungal isolates [1], however it is currently unknown if these isolates could present danger to the collections through bioweathering or secretion of organic compounds. We grew a strain of Fusarium oxysporium isolated from nitrogen gas filters feeding the Meteorite Lab nitrogen gas in the presence of a H5 meteorite thin section to determine if this fungus has the capability of altering the mineral structure of this common meteorite. This first trial was to determine and understand the effects of Fusarium oxysporum growth on the iron content within H5 chondrites and evaluate what additional components are needed in the development of future trial runs. This experiment determined new considerations for handling samples, the nature of microscopic scans before and after the incubation period, and the quality of the samples utilized for the experiment. The results of this experiment were promising and warrant further investigation with a more refined process and timeline.

Barbre, K. S. R.↗

NASA NTRSDate not supplied

Making Thin Sections from National Treasures: A Little Moon Rock Goes a Long Way

NASA’s Johnson Space Center curates the Apollo sample collection through their facilities in Houston, TX. It is a dual-purpose facility, intended both to store and preserve the precious samples, as well as to make them available for ongoing scientific study and public examination. The facilities consist of multiple vaults and lab spaces, including the Apollo Thin Section Lab. Although it is a part of the Curation facilities, this lab is intended to process specific lunar rock and dust samples into what are called “thin sections”. A thin section is a microscope slide with a very thin, highly polished slice of rock material mounted on it. These are used not only for microscope viewing, but also for a range of other sophisticated scientific instruments to map and measure fine details of the rock’s physical and chemical composition and structure. The process of making a thin section requires great care and patience and is an art in itself; each one is unique, and each sample can behave very differently while going through the same basic procedures. In general, it begins by taking a small chip of rock from a much larger sample. The rock fragment is placed within a small mold and liquid epoxy is poured over it and allowed to harden, producing what is called a “potted butt” (Figure 1). This is to stabilize the rock so that it won’t fragment or crumble during polishing, and to fill any cracks or voids within the rock. Once hardened, the epoxy on the bottom is carefully ground away to expose the rock surface within, which is then polished to a 1-micron finish. A thin new layer of fresh epoxy is then applied to the polished surface and used to mount it to a silica slide. Once the mounting epoxy has hardened, the potted butt is cut off less than one millimeter above the slide using a low-speed circular saw. This thin layer of sample material attached to the slide undergoes further grinding and polishing, typically bringing the sample thickness down to about 35 microns – roughly one third of the thickness of a human hair. The remaining potted butt is saved for future scientific investigations and can be re-polished and used again until all the rock material within it is used up. A single thin section can be reused countless times by numerous different researchers. In addition, thin sections are often exquisitely beautiful (Figure 2) and in some cases, lunar thin sections are used as public display samples, such as at the Smithsonian Air and Space Museum in Washington, DC. The production of thin sections allows an immense variety of research to be conducted on a tiny amount of rock or mineral material, allowing the bulk of the Apollo collection to stay pristine and unaltered, and thus, remain available for next generation of lunar scientists to further our insight into the Moon’s geological diversity, and to bring valuable new insights to our understanding of the origin of the Earth-Moon system.

J J Kent↗

NASA NTRSDate not supplied

ES2Vec: Earth Science Metadata Suggestions and Analogical Reasoning

As the volume of text-based Earth science research grows, it is increasingly possible to discover latent relationships in the literature. However, traditional methodologies are restricted by limited computational capabilities and intractable problem spaces. Advancements in natural language processing (NLP) have allowed us to use an extensive Earth science corpus to create a domain-specific word vector model, Es2Vec, which we have used to surface latent relationships between Earth science concepts and generate improved keyword tags. Earth science metadata keyword assignment is a challenging problem. Dataset curators select appropriate keywords from the Global Change Master Directory (GCMD) set of keywords. The keywords an are integral part of the search and discovery of these datasets. Hence, the selection of keywords is crucial to increasing the discoverability of datasets. Utilizing machine learning techniques, we provide users with automated keyword suggestions to complement manual selection. We trained a machine learning model that leverages the semantic embedding ability of Word2Vec models to process abstracts and suggest relevant keywords. A user interface tool we built to assist data curators in the assignment of such keywords is also described.

word vectors↗

NASA NTRSDate not supplied

Small Particle Glovebox Experiments - Preliminary Results

A substantial portion of the asteroid samples returned by the HAYABUSA2 and OSIRIS-REx missions will consist of small (submm) components (due to the collection of those particles on the asteroid surface and due to the fragmenting of larger, friable material post-collection). In order to minimize the alteration/oxidation of asteroid regolith, the bulk collections will be stored and curated in nitrogen purged gloveboxes. The processing of small particles in an N2 glovebox will present challenges that are different from those experienced during lunar and meteorite sample processing. Particles in this size range are susceptible to unpredictable electrostatic charging that can result in sample loss during processing operations. Methods for the handling of sub-mm particles have been well developed for environments with ambient atmospheric conditions and relative humidity (RH) ranges between 40-70%. In such conditions, a number of factors can be successfully employed to minimize the effects of triboelectric charging, including the use of Po-210 sources that neutralize excess charge and the utilization of conductive manipulation tools and sample substrates. However, relative humidity levels above 40% also contribute significantly to dissipation of triboelectric effects. We had not previously investigated our charge mitigation methods in a completely dry, nitrogen-purged environment, or whether they would be sufficient in enabling the successful processing of sub-mm samples. Current glovebox configurations in use in our lunar and meteorite curation laboratories are optimized for the processing of macroscopic samples and tools; these glovebox designs are likely unsuitable for the processing of collections for which the bulk collection is comprised of sub-mm components. Small particle sample preparation requires the use of an optical magnification instrument – typically a stereo binocular microscope with at least 20x magnification. Current glovebox designs are not optimized for the utilization or integration of stereo microscopes; while many current cabinets include microscope viewports that enable the use of small, externally mounted stereo microscopes, the focusing methods (usually involving the use of a lab jack) lack the fidelity and precision required for small particle manipulation and imaging. Working distances of higher (>50x magnification) objective lenses may preclude the external use of a stereo microscope through a viewport; in order to successfully manipulate and image very small (< 20m) particles, stereo and digital microscope systems that are integrated within the glovebox should be investigated. Finally, ergonomic considerations for small particle work within a glovebox must be considered to minimize risk of injury to sample processors. In order to investigate some of the unknown parameters relating to small particle processing within an N2 glovebox, we conducted preliminary, qualitative experiments utilizing a small lunar cabinet that was originally used for film development.

C. J. Snead↗

NASA NTRSDate not supplied

Expanding Biological Repository Data Available for Sharing and Knowledge Discovery

Biology has developed next-generation data science and alternative analytical approaches with methodologies which require principal investigator (PI) experimental assay data be re-used. This new approach involves mining multiple datasets at once from various hierarchical organizations of biological complexity, while concurrently evaluating how experimental factors affect endpoints of standard assays. The purpose of the NASA Ames Life Sciences Data Archive (ALSDA) is to collect, curate, and make findable, accessible, interoperable, and reusable (FAIR) all non-human space-relevant biological data. These data include mission metadata, subject metadata, assay metadata (parameters), raw and processed assay data, assay imagery, and subject-experienced telemetry (radiation, temperature, humidity, acoustics, vibrations). ALSDA has transformed to bring current biological repository data and all future collected data into this new scientific data mining reality. It has integrated into the ‘NASA Open Science’ group of projects to facilitate a suite of new tools and workflows to improve data accessibility and reusability by implementing data management plans, automating data submission agreements, and adopting the single-point-of-entry data submission portal, originally developed by NASA GeneLab. These systems required ALSDA to develop science assay configurations for the submission portal, capturing essential assay parameters according to established norms in each sub-field within biology. The submission portal expedites data collection by enhancing ease of PI data submission, providing a user interface and specificity for which data is to be submitted. ALSDA datasets are curated to maintain rich metadata, accuracy of datasets, data transparency, provenance, and additionally ensure data are machine-readable (e.g., R and Python languages). ALSDA integration with GeneLab and its analysis portals enable higher-order physiological-level datasets be mined in conjunction with -omics datasets. As ALSDA physiological-level datasets are published (micro-computed tomography, histology, intraocular pressure, hormonal assays, immunostaining, ultrasonography), the merging of hierarchical organizations of biological complexity from spaceflight will enable new knowledge discovery approaches.

Ryan T Scott↗

NASA NTRSDate not supplied

Hydrogen Peroxide as a Method for Bioburden Reduction in Facilities with Strict Materials Requirements.

The cleanrooms used to curate NASA’s Astromaterials samples are carefully monitored for particulate and inorganic contamination. The clean labs also have a very limited set of acceptable materials and cleaning agents to further minimize the potential for contamination. Labs are cleaned primarily with isopropyl alcohol. Astromaterials samples are handled with tools made of stainless steel (304 or 316), Teflon, or aluminum alloy (6061). Although our current collections are not particularly susceptible to biological alteration or organic contamination, this will not be the case for new collections from the OSIRIS-REx mission, Hayabusa2, and from Mars Sample Return. Therefore, it is necessary to develop and test methods to reduce the bioburden in astromaterials cleanrooms without introducing unwanted contaminants. We will report on the results of three case studies where 7.5 wt% hydrogen peroxide was prepared from a stock solution of ultrapure 30 wt% hydrogen peroxide (JT Baker) using curation-grade ultrapure water. We followed CDC (Center for Disease Control) guidelines for using hydrogen peroxide as a high level disinfectant. This solution was used to clean a glovebox prior to processing Apollo samples, as well as surfaces in the Antarctic meteorite processing lab and Stardust lab after facilities monitoring indicated an unwanted increase in bioburden. In all three instances, the culturable bioburden was significantly reduced after a 30 min. exposure to the 7.5% hydrogen peroxide solution without a corresponding increase in inorganic or organic contamination. We observed 77 to 100% reductions in the bioburden recovery rate. In one case study, we also performed amplicon DNA sequencing on samples collected from the surfaces before and after cleaning. We observed a significant change in microbial community composition after peroxide cleaning. These results suggest that routine cleaning with hydrogen peroxide could be an effective way to control bioburden in astromaterials cleanrooms and other facilities with strict contamination control requirements.

A B Regberg↗