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NASA NTRSDate not supplied

Neuro-Vestibular Examination During and Following Spaceflight (Vestibular Health)

BACKGROUND Adaptation to microgravity during spaceflight causes neurological disturbances that are either directly or indirectly mediated by the vestibular system. These disturbances could include space motion sickness, spatial disorientation, and cognitive impairment, as well as changes in head-eye coordination, vestibulo-ocular reflexes, and control of posture and locomotion. Otolith-mediated reflex gains appear to adapt rapidly during spaceflight and after landing. However, animal studies have shown that structural modifications of the vestibular sensory apparatus develop during long-duration spaceflight. To date, no studies have characterized the severity of vestibular syndromes experienced by astronauts as a function of the duration of spaceflight or whether the effects are caused by changes at the peripheral end organs, midbrain, cerebellum, or vestibular cortex. OBJECTIVES We will investigate temporal vestibular changes in crewmembers of short, 6-month, and one-year missions to identify trends in adaptation of vestibular health and performance in orbit and after landing. We will also determine whether the vestibular organs and/or the central vestibular system undergo structural changes during long-duration exposure to microgravity, which could cause vestibular disorders when transitioning to a different gravitational environment. METHODS Recordings of eye, head, and body movements, as well as subjective reports of perception of motion, will be used to determine the presence of abnormal eye movements, dysmetria, motion sickness symptoms, and illusions of motion during head or body movements. This includes characterization of temporal trends in central compensation for vestibular (otolith) asymmetry. Pre-flight data will be collected 90 days before the flight. In-flight tests will be performed early in the mission and once every one or two months thereafter. Post-flight examinations will be performed on the following days after return (R) from the mission: R+0, R+4, R+9, and R+30. Ground-based control tests will be performed on healthy volunteers in the laboratory to estimate mean normative responses. CONTROL RESULTS Thirty-two healthy (non-astronaut) control subjects performed the same ground test procedures as planned for crewmembers. In addition to establishing a normative database, these data were used to calculate vestibular asymmetries from perceptual reports during unilateral centrifugation, oculomotor responses during visual-alignment tasks, vestibulo-ocular reflex gain during head-impulse tests, and body rotation during stepping tests. A significant correlation was observed between asymmetries of subjective visual vertical and verbal report during unilateral centrifugation. Another significant correlation was observed between the asymmetries of ocular alignment, vestibulo-ocular reflex gain, and body rotation. These findings in a healthy cohort may help us better understand changes in vestibular asymmetries in crewmembers during and following spaceflight. RELEVANCE If the observed symptoms in crewmembers are more deleterious after the year-long missions than those documented after 6-month missions, then relevant countermeasures will be required to maintain the health and operational performance of astronauts during longer missions. Depending on the etiology of the vestibular syndrome revealed by these tests, countermeasures will be proposed based on vestibular rehabilitation therapies currently used in patients with vestibular disorders, such as habituation, gaze stabilization, and/or balance training exercises. ACKNOWLEDGEMENT This work is supported by the NASA’s Human Research Program Human Health Countermeasures Element.

T R Macaulay↗

NASA NTRSDate not supplied

Neuro-Vestibular Examination During and Following Spaceflight (Vestibular Health)

BACKGROUND Adaptation to microgravity during spaceflight causes neurological disturbances that are either directly or indirectly mediated by the vestibular system. These disturbances can include space motion sickness, spatial disorientation, and cognitive impairment, as well as changes in head-eye coordination, vestibulo-ocular reflexes, and control of posture and locomotion. Otolith-mediated reflex gains appear to adapt rapidly during spaceflight and after landing. However, animal studies have shown that structural modifications of the vestibular sensory apparatus develop during long-duration spaceflight. To date, no studies have characterized the severity of vestibular syndromes experienced by astronauts as a function of the duration of spaceflight or whether the effects are caused by changes at the peripheral end organs, midbrain, cerebellum, or vestibular cortex. OBJECTIVES We will investigate temporal vestibular changes in crewmembers of short, 6-month, and one-year missions to identify trends in adaptation of vestibular health and performance in orbit and after landing. We will also determine whether the vestibular organs and/or the central vestibular system undergo structural changes during long-duration exposure to microgravity, which could cause vestibular disorders when transitioning to a different gravitational environment. METHODS Recordings of eye, head, and body movements, as well as subjective reports of perception of motion, will be used to determine the presence of abnormal eye movements, dysmetria, motion sickness symptoms, and illusions of motion during head or body movements. This includes characterization of temporal trends in central compensation for vestibular (otolith) asymmetry. Pre-flight data will be collected 90 days before launch. In-flight examinations will be performed early in the mission (Flight Days 1 and 30) and once every two or three months thereafter. Post-flight examinations will be performed on the following days after return (R) from the mission: R+0, R+4, R+9, and R+30. Ground-based control tests have been performed on healthy volunteers in the laboratory to estimate mean normative responses. RESULTS Data collection for this study is ongoing. Data processing techniques are being refined. Our eye tracking method for measuring three-dimensional eye movement takes advantage of modern computer vision software (OpenCV) and advances in the field of iris recognition to improve measurement of ocular-counterolling. RELEVANCE If the observed symptoms in crewmembers are more deleterious after the year-long missions than those documented after 6-month missions, then relevant countermeasures will be required to maintain the health and operational performance of astronauts during longer missions. Depending on the etiology of the vestibular syndrome revealed by these tests, countermeasures will be proposed based on vestibular rehabilitation therapies currently used in patients with vestibular disorders, such as habituation, gaze stabilization, and/or balance training exercises.

T R Macaulay↗

NASA NTRS1987

How cereal grass shoots perceive and respond to gravity

The leaf-sheath pulvinus of grasses presents a unique system for studying gravitropism, primarily because of its differences from other organs. The mature pulvinus is a discrete organ specialized for gravitropism: it is nongrowing in the absence of gravistimulation and capable of displaying a graviresponse independent of the rest of the plant. In this paper we present a model for gravitropism in pulvini based on recent findings from studies on the mechanisms of graviperception and graviresponse. According to this model, amyloplasts play an essential role in perceiving a change in the orientation of the pulvinus. The perception of this reorientation leads to the enhanced synthesis and release from conjugate of the auxin IAA, and the increased conjugation of gibberellin, on a localized basis. Because there is a graded growth promotion across the gravistimulated pulvinus, it is suggested that the observed hormonal asymmetry is actually an indication of a linear gradient of hormone concentration, as well as hormone response, across the pulvinus. It is further suggested that the linear gradient of hormone concentration may be predominantly the result of local changes in hormone level, rather than a product of hormonal movement into or across the pulvinus.

NASA Discipline Number 40-99↗

NASA NTRSDate not supplied

4pPA1 - Turbulence Effects on Shaped Booms: Central Composite Design of Modeled Atmospheric Turbulence Parameters for Sonic Boom Propagation

Propagation of sonic booms through turbulence reduces mean sonic boom perception metric levels and also causes considerable variability. NASA’s PCBoom suite of sonic boom acoustic propagation modules includes an approximate method for accounting for the effects of turbulence on traditional N-wave sonic booms. The current implementation is ineffective for shaped sonic booms or low-booms, and it also has limited values for turbulence and ambient input parameters. NASA’s future X-59 low-boom community noise surveys require an accurate estimate of the effects of turbulence in regions across the USA, so the module must be improved. This work presents the methods of selecting which ambient and turbulence parameters should be included in an improved PCBoom turbulence module. Turbulence and ambient data were collected from two atmospheric model databases, the Climate Forecast System Version 2 and European Centre for Medium-Range Weather Forecast Reanalysis Version 5 (ERA5), hourly from 7 AM to 7 PM local time for 10 years at 19 locations across the USA. A fully-factorial propagation analysis using these parameters would be exceedingly computationally expensive. Instead, a central composite design was chosen resulting in 45 combinations of ambient and turbulence parameters. These 45 cases effectively sample the space balancing computational burden.

turbulence↗

NASA NTRS2000

Augmentation of blood circulation to the fingers by warming distant body areas

Future activities in space will require greater periods of time in extreme environments in which the body periphery will be vulnerable to chilling. Maintaining the hands and fingers in comfortable conditions enhances finger flexibility and dexterity, and thus effects better work performance. We have evaluated the efficacy of promoting heat transfer and release by the extremities by increasing the blood flow to the periphery from more distant parts of the body. The experimental garment paradigm developed by the investigators was used to manipulate the temperature of different body areas. Six subjects, two females and four males, were evaluated in a stage-1 baseline condition, with the inlet temperature of the circulating water in the liquid cooling/warming garment (LCWG) at 33 degrees C. At stage 2 the total LCWG water inlet temperature was cooled to 8 degrees C, and at stage 3 the inlet water temperature in specific segments of the LCWG was warmed (according to protocol) to 45 degrees C, while the inlet temperature in the rest of the LCWG was maintained at 8 degrees C. The following four body-area-warming conditions were studied in separate sessions: (1) head, (2) upper torso/arm, (3) upper torso/arm/head, and (4) legs/feet. Skin temperature, heat flux and blood perfusion of the fingers, and subjective perception of thermal sensations and overall physical comfort were assessed. Finger temperature (T(fing)) analyses showed a statistically significant condition x stage interaction. Post-hoc comparisons (T(fing)) indicated that at stage 3, the upper torso/arm/head warming condition was significantly different from the head, upper torso/arm and legs/feet conditions, showing an increase in T(fing). There was a significant increase in blood perfusion in the fingers at stage 3 in all conditions. Subjective perception of hand warmth, and overall physical comfort level significantly increased in the stage 3 upper torso/arm/head condition. The findings indicate that physiological methods to enhance heat transfer by the blood to the periphery within protective clothing provide an additional tool for increasing total and local human comfort in extreme environments.

NASA Discipline Life Sciences Technologies↗

NASA NTRS2016

Space Suit Portable Life Support System (PLSS) 2.0 Human-in-the-Loop (HITL) Testing

The space suit Portable Life Support System (PLSS) 2.0 represents the second integrated prototype developed and tested to mature a design that uses advanced technologies to reduce consumables, improve robustness, and provide additional capabilities over the current state of the art. PLSS 2.0 was developed in 2012, with extensive functional evaluations and system performance testing through mid-2014. In late 2014, PLSS 2.0 was integrated with the Mark III space suit in an ambient laboratory environment to facilitate manned testing, designated PLSS 2.0 Human-in-the-Loop (HITL) testing, in which the PLSS prototype performed the primary life support functions, including suit pressure regulation, ventilation, carbon dioxide control, and cooling of the test subject and PLSS avionics. The intent of this testing was to obtain subjective test subject feedback regarding qualitative aspects of PLSS 2.0 performance such as thermal comfort, sounds, smells, and suit pressure fluctuations due to the cycling carbon dioxide removal system, as well as to collect PLSS performance data over a range of human metabolic rates from 500-3000 Btu/hr. Between October 27 and December 18, 2014, nineteen two-hour simulated EVA test points were conducted in which suited test subjects walked on a treadmill to achieve a target metabolic rate. Six test subjects simulated nominal and emergency EVA conditions with varied test parameters including metabolic rate profile, carbon dioxide removal control mode, cooling water temperature, and Liquid Cooling and Ventilation Garment (state of the art or prototype). The nineteen test points achieved more than 60 hours of test time, with 36 hours accounting for simulated EVA time. The PLSS 2.0 test article performed nominally throughout the test series, confirming design intentions for the advanced PLSS. Test subjects' subjective feedback provided valuable insight into thermal comfort and perceptions of suit pressure fluctuations that will influence future advanced PLSS design and testing strategies.

Watts, Carly↗

NASA NTRS2016

Space Suit Portable Life Support System (PLSS) 2.0 Human-in-the-Loop (HITL) Testing

Watts, Carly↗

DOE OSTI2025

New Mobility Technology Assessment - Benefits, Risks, and Safety

The age of vehicle automation is upon us. The fundamental perception and control technology is maturing as we speak (and write) with robo-taxis proliferating in select US cities. The news cycle is no longer filled with technological skepticism, but rather societal commentary on our collective response and use of the technology. This maturing of the technology is also accompanied by a renaissance in new types of urban movement technology to more effectively link people with goods, services, and employment, particularly in dense development areas where vehicle congestion (even automated vehicles) prevents high quality mobility. The National Renewable Energy Laboratory, through the DOE Clean Energy to Communities (C2C) program has worked with two jurisdictions to assess the benefits, risks, and safety concerns for a new product offering based on suspended cable technology which is fully automated and proposes an aerial network of self-propelled gondolas. The recognized need, independent of the technology, is to fairly assess the benefits of the proposed transportation system, assess its technical maturity, as well as risks and safety concerns. Using this new technology as an example, a technology represented by Swyft Cities, NREL developed a succession of mini-studies that characterized the technical maturity level, assessed the proposed benefits for Greenville, SC, examined the risks involved in moving forward with an emerging mobility technology, and determined the governing safety protocols and standards that are applicable to the space. These mini-case studies, though centered on Swyft Cities aerial automated gondola technology, are highly transferable to any type of new mobility technology and lays out a repeatable process for assessing new technology within an city or community context.

33 ADVANCED PROPULSION SYSTEMS↗

NASA NTRS2001

A dissociation between attention and selection

It is widely assumed that the allocatian of spatial attention results in the "selection" of attended objects or regions of space. That is, once a stimulus is attended, all its feature dimensions are processed irrespective of their relevance to behavioral goals. This assumption is based in part on experiments showing significant interference for attended stimuli when the response to an irrelevant dimension conflicts with the response to the relevant dimension (e.g., the Stroop effect). Here we show that such interference is not due to attending per se. In two spatial cuing experiments, we found that it was possible to restrict processing of attended stimuli to task-relevant dimensions. This new evidence supports two novel conclusions: (a) Selection involves more than the focusing of attention per se: and (b) task expectations play a key role in detertnining the depth of processing of the elementary feature dimensions of attended stimuli.

Attention↗

NASA NTRS1990

Perception of linear acceleration in weightlessness

Tests of the perception and use of linear acceleration sensory information were performed on the science crews of the Spacelab 1 (SL-1) and D-1 missions using linear "sleds" in-flight (D-1) and pre-post flight. The time delay between the acceleration step stimulus and the subjective response was consistently reduced during weightlessness, but was neither statistically significant nor of functional importance. Increased variability of responses when going from one environment to the other was apparent from measurements on the first day of the mission and in the first days post-flight. Subjective reports of perceived motion during sinusoidal oscillation in weightlessness were qualitatively similar to reports on earth. In a closed-loop motion nulling task, enhanced performance was observed post-flight in all crewmembers tested in the Y or Z axes.

NASA Discipline Number 16-10↗

NASA NTRS1997

Mechanical signals in plant development: a new method for single cell studies

Cell division, which is critical to plant development and morphology, requires the orchestration of hundreds of intracellular processes. In the end, however, cells must make critical decisions, based on a discrete set of mechanical signals such as stress, strain, and shear, to divide in such a way that they will survive the mechanical loads generated by turgor pressure and cell enlargement within the growing tissues. Here we report on a method whereby tobacco protoplasts swirled into a 1.5% agarose entrapment medium will survive and divide. The application of a controlled mechanical load to agarose blocks containing protoplasts orients the primary division plane of the embedded cells. Photoelastic analysis of the agarose entrapment medium can identify the lines of principal stress within the agarose, confirming the hypothesis that cells divide either parallel or perpendicular to the principal stress tensors. The coincidence between the orientation of the new division wall and the orientation of the principal stress tensors suggests that the perception of mechanical stress is a characteristic of individual plant cells. The ability of a cell to determine a shear-free orientation for a new partition wall may be related to the applied load through the deformation of the matrix material. In an isotropic matrix a uniaxial load will produce a rotationally symmetric strain field, which will define a shear-free plane. Where high stress intensities combine with the loading geometry to produce multiaxial loads there will be no axis of rotational symmetry and hence no shear free plane. This suggests that two mechanisms may be orienting the division plane, one a mechanism that works in rotationally symmetrical fields, yielding divisions perpendicular to the compressive tensor, parallel to the long axis of the cell, and one in asymmetric fields, yielding divisions parallel to the short axis of the cell and the compressive tensor.

NASA Discipline Number 40-50↗

NASA NTRS2005

Middleware Evaluation and Benchmarking for Use in Mission Operations Centers

Middleware technologies have been promoted as timesaving, cost-cutting alternatives to the point-to-point communication used in traditional mission operations systems. However, missions have been slow to adopt the new technology. The lack of existing middleware-based missions has given rise to uncertainty about middleware's ability to perform in an operational setting. Most mission architects are also unfamiliar with the technology and do not know the benefits and detriments to architectural choices - or even what choices are available. We will present the findings of a study that evaluated several middleware options specifically for use in a mission operations system. We will address some common misconceptions regarding the applicability of middleware-based architectures, and we will identify the design decisions and tradeoffs that must be made when choosing a middleware solution. The Middleware Comparison and Benchmark Study was conducted at NASA Goddard Space Flight Center to comprehensively evaluate candidate middleware products, compare and contrast the performance of middleware solutions with the traditional point- to-point socket approach, and assess data delivery and reliability strategies. The study focused on requirements of the Global Precipitation Measurement (GPM) mission, validating the potential use of middleware in the GPM mission ground system. The study was jointly funded by GPM and the Goddard Mission Services Evolution Center (GMSEC), a virtual organization for providing mission enabling solutions and promoting the use of appropriate new technologies for mission support. The study was broken into two phases. To perform the generic middleware benchmarking and performance analysis, a network was created with data producers and consumers passing data between themselves. The benchmark monitored the delay, throughput, and reliability of the data as the characteristics were changed. Measurements were taken under a variety of topologies, data demands, and data characteristics, using several middleware products. All results were compared to systems using traditional point-to-point socket communication. By comparing performance results under Merent settings, inferences could be made about each middleware's ability to meet certain requirements of the GPM mission. The second phase simulated a middleware-based mission operations center. Actual mission support tools were either used or simulated to create real world demands on the middleware. Network and computer demands were watched closely to verify that no specific idiosyncrasies of mission operations activities would prove unsupportable by the middleware. In our presentation, we will address some commonly accepted misconceptions concerning middleware in mission support architectures. Specifically, we will focus on the perception that middleware solutions are too slow or impose too much overhead for real-time mission operations, and that middleware solutions are too expensive for small

Antonucci, Rob↗

NASA NTRS2017

Color Algebras

A color algebra refers to a system for computing sums and products of colors, analogous to additive and subtractive color mixtures. We would like it to match the well-defined algebra of spectral functions describing lights and surface reflectances, but an exact correspondence is impossible after the spectra have been projected to a three-dimensional color space, because of metamerism physically different spectra can produce the same color sensation. Metameric spectra are interchangeable for the purposes of addition, but not multiplication, so any color algebra is necessarily an approximation to physical reality. Nevertheless, because the majority of naturally-occurring spectra are well-behaved (e.g., continuous and slowly-varying), color algebras can be formulated that are largely accurate and agree well with human intuition. Here we explore the family of algebras that result from associating each color with a member of a three-dimensional manifold of spectra. This association can be used to construct a color product, defined as the color of the spectrum of the wavelength-wise product of the spectra associated with the two input colors. The choice of the spectral manifold determines the behavior of the resulting system, and certain special subspaces allow computational efficiencies. The resulting systems can be used to improve computer graphic rendering techniques, and to model various perceptual phenomena such as color constancy.

color constancy↗

NASA NTRS2023

Designing Learning Experiences With A Low-Cost Robotic Arm

Robots have gained immense popularity in Hollywood and growth globally in both industrial manufacturing and non-manufacturing environments and applications such as healthcare, service sector, and space exploration. To date, there are several examples of simple and low-cost educational robotic platforms and commercially available platforms (e.g., Lego Mindstorms, VEX Robotics) for incorporation into existing curriculum. However, most low-cost examples require access to rapid-prototyping tools, such as 3D printers to manufacture the structure of the robot, while commercially available platforms are relatively expensive (> $1,000). Although the low-cost, open-source examples provide increased access, these examples require design and manufacturing tasks that would be considered outside the learning objectives of an upper-level robotics course and are better suited for other introductory courses. Thus, we asked, how can a robotic platform be incorporated into existing robotics curriculum to enhance students' learning experiences? To explore this research question, we introduce a low-cost (<$200), untethered, and transportable robotic platform that is easy to assemble using off-the-shelf components. This kit can be powered from a laptop computer and does not rely on access to rapid-prototyping tools such as 3D printers or laser cutters, making this a more accessible option in undergraduate engineering courses. Specifically, we aimed to investigate the design of experiential learning experiences for the mathematical modeling of the forward and inverse kinematics of a serial robotic arm that complements existing robotics curriculum. The experiential learning experience focuses on traditional written answer, simulation in MATLAB, and finally implementation on a robotic platform. Few studies examined this accessible option when evaluating experiential learning experiences that complement existing robotics curricula. To assess the impact of this robotic arm kit in an undergraduate course, we implemented an educational intervention that allowed us insight into student perceptions, takeaways on the course and activities involving the robotic arm, and the impact of the course on their career outlook when comparing activities that involved use of the robotic arm and those that did not. Details on the design, development and implementation of the learning activities is provided. Both quantitative and qualitative data were collected and analyzed. The results presented in this paper discuss students finding the learning activities on the robotic arms more helpful than those without, and that students found high value in the hands-on experiences and real-world scenarios offered by the activities using the robotic arm. Challenges to implementation of the robotic arms are discussed, including students’ prior knowledge of using robotic arms.

Eric J. Markvicka↗

NASA NTRS1986

M.I.T./Canadian vestibular experiments on the Spacelab-1 mission: 6. Vestibular reactions to lateral acceleration following ten days of weightlessness

Tests of otolith function were performed pre-flight and post-flight on the science crew of the first Spacelab Mission with a rail-mounted linear acceleration sled. Four tests were performed using horizontal lateral (y-axis) acceleration: perception of linear motion, a closed loop nulling task, dynamic ocular torsion, and lateral eye deviations. The motion perception test measured the time to detect the onset and direction of near threshold accelerations. Post-flight measures of threshold and velocity constant obtained during the days immediately following the mission showed no consistent pattern of change among the four crewmen compared to their pre-flight baseline other than an increased variability of response. In the closed loop nulling task, crewmen controlled the motion of the sled and attempted to null a computer-generated random disturbance motion. When performed in the light, no difference in ability was noted between pre-flight and post-flight. In the dark, however, two of the four crewmen exhibited somewhat enhanced performance post-flight. Dynamic ocular torsion was measured in response to sinusoidal lateral acceleration which produces a gravitionertial stimulus equivalent to lateral head tilt without rotational movement of the head. Results available for two crewmen suggest a decreased amplitude of sinusoidal ocular torsion when measured on the day of landing (R+0) and an increasing amplitude when measured during the week following the mission.

short duration↗

NASA NTRS1994

Physiological and genetic characterization of plant growth and gravitropism in LED light sources

Among the many problems of growing plants in completely controlled environments, such as those anticipated for the space station and the CELSS program, is the need to provide light that is both adequate for photosynthesis and of proper quality for normal growth and development. NASA scientists and engineers have recently become interested in the possibility of utilizing densely packed, solid state, light emitting diodes (LED's) as a source for this light. Unlike more conventional incandescent or electrical discharge lamps, these sources are highly monochromatic and lack energy in spectral regions thought to be important for normal plant development. In addition, a recent observation by NASA scientist has suggested that infra-red LED's, that are routinely used as photographic safelights for plants grown in darkness, may interact with the ability of plants to detect gravity. In order to establish how plants respond to light from these LED light sources we carried out a series of experiments with known pigment mutants of the model mustard plant, Arabidopsis thaliana, growing in either a gravity field or on a clinostat to simulate a micro-gravity environment. Results indicate that only red light from the 665 nm LED's disrupts the ability of normal wildtype seedlings to detect a gravity stimulus. There was no consistent effect found for the far-red (735 nm) LED's or either of the infrared (880 nm or 935 nm) LED sources but both showed some effect in one or more of the genotypes tested. Of these five members of the phytochrome multigene family in Arabidopsis, only the phytochrome B pigment mutant (hy3) lacked the ability to detect gravity under all conditions. There was no effect of either micro-gravity (clinostat) or the infra-red LED's on the light induced inhibition of hypocotyl elongation. Measurements of the pigment phytochrome in oats also showed no photoconversion by 15 min irradiations with the infra-red LED's. We conclude that phytochrome B is required for the perception of gravity and that only red light is able to disrupt this perception. The infra-red LED's also do not appear to interact with gravity perception in Arabidopsis, but caution should be exercised if infra-red LED's are to be used as photographic safelights for these types of experiments.

Deitzer, Gerald F.↗

NASA NTRS1989

Auxin physiology of the tomato mutant diageotropica

The tomato (Lycopersicon esculentum, Mill.) mutant diageotropica (dgt) exhibits biochemical, physiological, and morphological abnormalities that suggest the mutation may have affected a primary site of auxin perception or action. We have compared two aspects of the auxin physiology of dgt and wild-type (VFN8) seedlings: auxin transport and cellular growth parameters. The rates of basipetal indole-3-acetic acid (IAA) polar transport are identical in hypocotyl sections of the two genotypes, but dgt sections have a slightly greater capacity for IAA transport. 2,3,5-Triiodobenzoic acid and ethylene reduce transport in both mutant and wild-type sections. The kinetics of auxin uptake into VFN8 and dgt sections are nearly identical. These results make it unlikely that an altered IAA efflux carrier or IAA uptake symport are responsible for the pleiotropic effects resulting from the dgt mutation. The lack of auxin-induced cell elongation in dgt plants is not due to insufficient turgor, as the osmotic potential of dgt cell sap is less (more negative) than that of VFN8. An auxin-induced increase in wall extensibility, as measured by the Instron technique, only occurs in the VFN8 plants. These data suggest dgt hypocotyls suffer a defect in the sequence of events culminating in auxin-induced cell wall loosening.

NASA Discipline Plant Biology↗

NASA NTRS1991

Regulation of phytochrome message abundance in root caps of maize

In many cultivars of maize (Zea mays L.) red light affects root development via the photomorphogenetic pigment phytochrome. The site of perception for the light is the root cap. In the maize cultivar Merit, we investigated phytochrome-mediated events in the cap. We established that the message encoded by the phyA1 gene was most abundant in dark-grown tissue and was asymmetrically distributed in the root cap, with greatest expression in the cells which make up the central columella core of the cap. Phytochrome message was negatively autoregulated in a specific region within the root cap. This autoregulation was sensitive to very-low-fluence red light, and thus was characterized as a phytochrome-mediated, very-low-fluence event. The kinetics of message reaccumulation in the dark were also examined and compared to the kinetics of the light requirement for root gravitropism in this cultivar. Similarly, the degree of autoregulation present in two other maize cultivars with different light requirements for gravitropic sensitivity was investigated. It appears that the Merit cultivar expresses a condition of hypersensitivity to phytochrome-mediated light regulation in root tissues. We conclude that phytochrome regulates many activities within the cap, but the degree to which these activities share common phytochrome-mediated steps is not known.

NASA Discipline Plant Biology↗