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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 451 records · Page 25

Transposon tagging and the study of root development in Arabidopsis

The maize Ac-Ds transposable element family has been used as the basis of transposon mutagenesis systems that function in a variety of plants, including Arabidopsis. We have developed modified transposons and methods which simplify the detection, cloning and analysis of insertion mutations. We have identified and are analyzing two plant lines in which genes expressed either in the root cap cells or in the quiescent cells, cortex/endodermal initial cells and columella cells of the root cap have been tagged with a transposon carrying a reporter gene. A gene expressed in root cap cells tagged with an enhancer-trap Ds was isolated and its corresponding EST cDNA was identified. Nucleotide and deduced amino acid sequences of the gene show no significant similarity to other genes in the database. Genetic ablation experiments have been done by fusing a root cap-specific promoter to the diphtheria toxin A-chain gene and introducing the fusion construct into Arabidopsis plants. We find that in addition to eliminating gravitropism, root cap ablation inhibits elongation of roots by lowering root meristematic activities.

NASA Discipline Plant Biology↗

Composite x-ray image assembly for large-field digital mammography with one- and two-dimensional positioning of a focal plane array

To demonstrate the feasibility of a novel large-field digital mammography technique, a 1024 x 1024 pixel Loral charge-coupled device (CCD) focal plane array (FPA) was positioned in a mammographic field with one- and two-dimensional scan sequences to obtain 950 x 1800 pixel and 3600 x 3600 pixel composite images, respectively. These experiments verify that precise positioning of FPAs produced seamless composites and that the CCD mosaic concept has potential for high-resolution, large-field imaging. The proposed CCD mosaic concept resembles a checkerboard pattern with spacing left between the CCDs for the driver and readout electronics. To obtain a complete x-ray image, the mosaic must be repositioned four times, with an x-ray exposure at each position. To reduce the patient dose, a lead shield with appropriately patterned holes is placed between the x-ray source and the patient. The high-precision motorized translation stages and the fiber-coupled-scintillating-screen-CCD sensor assembly were placed in the position usually occupied by the film cassette. Because of the high mechanical precision, seamless composites were constructed from the subimages. This paper discusses the positioning, image alignment procedure, and composite image results. The paper only addresses the formation of a seamless composite image from subimages and will not consider the effects of the lead shield, multiple CCDs, or the speed of motion.

Mammography/methods/statistics & numerical data↗

Tow-Steered Panels With Holes Subjected to Compression or Shear Loads

Tailoring composite laminates to vary the fiber orientations within a fiber layer of a laminate to address non-uniform stress states and provide structural advantages such as the alteration of principal load paths has potential application to future low-cost, light-weight structures for commercial transport aircraft. Evaluation of this approach requires the determination of the effectiveness of stiffness tailoring through the use of curvilinear fiber paths in flat panels including the reduction of stress concentrations around the holes and the increase in load carrying capability. Panels were designed through the use of an optimization code using a genetic algorithm and fabricated using a tow-steering approach. Manufacturing limitations, such as the radius of curvature of tows the machine could support, avoidance of wrinkling of fibers and minimization of gaps between fibers were considered in the design process. Variable stiffness tow-steered panels constructed with curvilinear fiber paths were fabricated so that the design methodology could be verified through experimentation. Finite element analysis where each element s stacking sequence was accurately defined is used to verify the behavior predicted based on the design code. Experiments on variable stiffness flat panels with central circular holes were conducted with the panels loaded in axial compression or shear. Tape and tow-steered panels are used to demonstrate the buckling, post-buckling and failure behavior of elastically tailored panels. The experimental results presented establish the buckling performance improvements attainable by elastic tailoring of composite laminates.

Jegley, Dawn C.↗

MSLICE Science Activity Planner for the Mars Science Laboratory Mission

MSLICE (Mars Science Laboratory InterfaCE) is the tool used by scientists and engineers on the Mars Science Laboratory rover mission to visualize the data returned by the rover and collaboratively plan its activities. It enables users to efficiently and effectively search all mission data to find applicable products (e.g., images, targets, activity plans, sequences, etc.), view and plan the traverse of the rover in HiRISE (High Resolution Imaging Science Experiment) images, visualize data acquired by the rover, and develop, model, and validate the activities the rover will perform. MSLICE enables users to securely contribute to the mission s activity planning process from their home institutions using off-the-shelf laptop computers. This software has made use of several plug-ins (software components) developed for previous missions [e.g., Mars Exploration Rover (MER), Phoenix Mars Lander (PHX)] and other technology tasks. It has a simple, intuitive, and powerful search capability. For any given mission, there is a huge amount of data and associated metadata that is generated. To help users sort through this information, MSLICE s search interface is provided in a similar fashion as major Internet search engines. With regard to the HiRISE visualization of the rover s traverse, this view is a map of the mission that allows scientists to easily gauge where the rover has been and where it is likely to go. The map also provides the ability to correct or adjust the known position of the rover through the overlaying of images acquired from the rover on top of the HiRISE image. A user can then correct the rover s position by collocating the visible features in the overlays with the same features in the underlying HiRISE image. MSLICE users can also rapidly search all mission data for images that contain a point specified by the user in another image or panoramic mosaic. MSLICE allows the creation of targets, which provides a way for scientists to collaboratively name features on the surface of Mars. These targets can also be used to convey instrument-pointing information to the activity plan. The software allows users to develop a plan of what they would like the rover to accomplish for a given time period. When developing the plan, the user can input constraints between activities or groups of activities. MSLICE will enforce said constraints and ensure that all mission flight rules are satisfied.

Powell, Mark W.↗

Abiotic Synthesis of Nucleic Acids: Hypochromicity and Future Research

The earliest forms of life would likely have a protocellular form, with a membrane encapsulating some form of linear charged polymer. These polymers could have enzymatic as well as genetic properties. We can simulate plausible prebiotic conditions in the laboratory to test hypotheses related to this concept. In earlier work we have shown that mononucleotides organized within a multilamellar lipid matrix can produce oligomers in the anhydrous phase of dehydration- rehydration cycles (Rajamani, 2008). If mononucleotides are in solution at millimolar concentrations, then oligomers resembling RNA are synthesized and exist in a steady state with their monomers DeGuzman, 2014). We have used conventional and novel techniques to demonstrate that secondary structures stabilized by hydrogen bonds may be present in the condensation products produced in dehydration- rehydration cycles that simulate hydrothermal fields that were present on the early Earth. Gel electrophoresis data corroborates the presence of up to 200-base pair length RNA fragments in products of Hydration-Dehydration experiments. Furthermore, hypochromicity measurements demonstrate a degree of hypochromicity found in single RNA strand of known sequence, as well as results that indicate this is true also for a sample of complementary strands of RNA. Analysis of ionic current signatures of known RNA hairpin molecule as measured using a nanopore detector indicate a significant variability in pattern, different from the signatures produced by DNA hairpin molecules. This informs how we may interpret nanopore data gathered from prebiotic simulations.

Glass, K.↗

NASA Open Science Data Repository: Maximizing Spaceflight Bioscience Data

The next era in human space exploration is rapidly approaching and will require the use of countermeasures to deep space health hazards. The development of countermeasures (or, the re-purposing of existing agents) will be highly dependent on our understanding of basic biological responses to space stressors (e.g. ionizing radiation, altered gravitational fields, altered day-night cycles, confinement, isolation, hostile-closed environments, distance-duration from Earth, exposure to celestial regolith, etc.). The fast-growing array of space biological data, which in the past was simply archived after minimal analysis, holds great potential if it can be reorganized and formatted for data re-analysis and re-use via Open Science. Organizing the data for such analysis is a challenge because of its diverse nature (molecular, cellular, tissue, imaging, whole organism and behavior). To address the challenges posed by gaining new knowledge from a vast and diverse amount of biological, health and environmental data in space, the NASA Open Science Data Repository (OSDR - osdr.nasa.gov/bio) plays a crucial role in curating and openly publishing biological data from space-related experiments. Its design incorporates successes and lessons from NASA GeneLab, encompassing not only high-throughput sequencing data but also physiological, phenotypic, and telemetry data. The OSDR makes space biological data FAIR (findable, accessible, interoperable, reusable), and facilitates effective data ingestion, dissemination, and Open Science collaborations. The OSDR also has the capability to integrate human astronaut data with state-of-the-art security and accessibility procedures. We will discuss here several strategies that NASA’s Biological and Physical Science Division have put in place to maximize the return on investment for spaceflight bioscience data.

space biology↗

On the angular momentum loss of late-type stars

The observed surface angular velocity of main-sequence stars shows a sharp decrease at about spectral type F6. It is suggested that stars more massive than F6 cannot experience an appreciable angular-momentum loss because their convection zones cannot sustain a magnetic dynamo: without a magnetic field the angular-momentum loss is very small. The influence of rotation on the convective motions is essential for the existence of a solar-type dynamo. Rotation can influence these convective motions only if the typical convective time is larger than the rotation time. For main-sequence stars of different masses and chemical compositions the dimensionless parameter (convective velocity/sum's angular velocity times mixing length in the lower part of the convection zone) is evaluated. It is shown that this parameter increases very sharply for stars whose mass exceeds that defined by the relation log(star mass/solar mass) is of the order of 0.1. Thus even for large angular velocities, magnetic dynamos are not feasible if log(star mass/solar mass) appreciably exceeds 0.1.

Durney, B. R.↗

Cell-selectivity of tryptophan and tyrosine in amphiphilic α-helical antimicrobial peptides against drug-resistant bacteria

Highlights: • Novel peptides showed antibacterial activity in dose- and length-dependent manner. • All peptides showed membranolytic action, although mechanistic details may differ. • Most peptides were non-toxic to mammalian cells. • Presence of Trp and Tyr increased cell-specificity and antibacterial activity. The increasing emergence of drug-resistant bacteria creates a requirement for new antibiotics and various types of antibiotic materials such as proteins, peptides, polymers, and chemical compounds. Among these, antimicrobial peptides (AMPs) are considered to be promising antibiotic candidates for clinical treatments. In this study, we have designed a novel series of peptides with repeated sequences of minimum membrane-active motif, ‘XWZX’ basic sequence (X: lysine or arginine, Z: leucine, tyrosine, valine, or glycine), and an α-helical secondary structure. Some peptides displayed a potent antibacterial activity via membranolytic action and high therapeutic index (toxic dose/minimum inhibitory concentration) in vitro. Furthermore, in vivo experiments using bacterial ear-skin infection models verified that these peptides have the potential to be powerful and safe antibiotics. The present study provides a lead sequence for designing peptide antibiotics against bacterial membranes and information for cell-selectivity of hydrophobic amino acids with aromatic side chains such as Trp and Tyr.

60 APPLIED LIFE SCIENCES↗

Viking X-band telemetry experiment

In order to uncover operational and design problems in the use of X-band by the 1977 Mariner Jupiter-Saturn mission and future spacecraft using the Deep Space Network, the Viking X-band telemetry experiment was conducted. The experiment was conducted during the months of December 1975 and January 1976. During each of the five successful passes, a periodic sequence (in lieu of ranging) was transmitted to the spacecraft and returned by the spacecraft transponder on both S- and X-bands. These telemetry-like signals were received, demodulated, and detected. From a variety of measurements at the station, four independent measurements were made of the received signal-to-noise ratio (SNR). These four SNRs were later compared with each other and the predicted SNR. The principal result of the experiment is that X-band telemetry works as expected. That is, the measured SNRs were consistent relative to each other and to the predicted values within the accuracy of the experiment.

Weber, W. J., III↗

Intensity of sample processing methods impacts wastewater SARS-CoV-2 whole genome amplicon sequencing outcomes

Wastewater SARS-CoV-2 surveillance has been deployed since the beginning of the COVID-19 pandemic to monitor the dynamics in virus burden in local communities. Genomic surveillance of SARS-CoV-2 in wastewater, particularly efforts aimed at whole genome sequencing for variant tracking and identification, are still challenging due to low target concentration, complex microbial and chemical background, and lack of robust nucleic acid recovery experimental procedures. The intrinsic sample limitations are inherent to wastewater and are thus unavoidable. Here, we use a statistical approach that couples correlation analyses to a random forest-based machine learning algorithm to evaluate potentially important factors associated with wastewater SARS-CoV-2 whole genome amplicon sequencing outcomes, with a specific focus on the breadth of genome coverage. We collected 182 composite and grab wastewater samples from the Chicago area between November 2020 to October 2021. Samples were processed using a mixture of processing methods reflecting different homogenization intensities (HA + Zymo beads, HA + glass beads, and Nanotrap), and were sequenced using one of the two library preparation kits (the Illumina COVIDseq kit and the QIAseq DIRECT kit). Technical factors evaluated using statistical and machine learning approaches include sample types, certain sample intrinsic features, and processing and sequencing methods. The results suggested that sample processing methods could be a predominant factor affecting sequencing outcomes, and library preparation kits was considered a minor factor. Finally, a synthetic SARS-CoV-2 RNA spike-in experiment was performed to validate the impact from processing methods and suggested that the intensity of the processing methods could lead to different RNA fragmentation

60 APPLIED LIFE SCIENCES↗

Assessment of Critical Experiment Benchmark Applicability to a Large-Capacity HALEU Transportation Package Concept

This work presents an assessment of the applicability of existing benchmark critical experiments to the criticality safety code validation for a large-capacity high-assay low-enriched uranium (HALEU) transportation package concept. Numerous next-generation nuclear reactor designs require HALEU fuel, which is characterized by an enrichment between 5 and 20 wt% 235 U. The U.S. Department of Energy (DOE) has proposed to recover and downblend highly enriched uranium from DOE-owned used nuclear fuel to accelerate the demonstration of commercially viable microreactor technologies. One element of the infrastructure needed to demonstrate HALEU-fueled reactors is the ability to safely transport enriched product to be used for fuel fabrication. There is uncertainty as to whether existing critical benchmark experiment data are sufficient to support criticality safety code validation for HALEU transportation applications. The anticipated chemical form of the HALEU in the proposed transportation concept is UO 2 with 20 wt% 235 U/U. The concept uses a combination of an existing transportation packaging design and a novel basket design, including borated aluminum flux traps. The basket provides space for 18 reusable, stainless steel canisters that contain the HALEU. In 10 CFR 71, normal conditions of transport (NCTs) and hypothetical accident conditions (HACs) are defined for fissile material transportation packages. NCT and HAC KENO-VI models of the transportation package were developed using the Standardized Computer Analyses for Licensing Evaluation (SCALE) 6.2.3 computer code package, and optimum moderation conditions were determined using the SCALE SAMPLER sequence. The SCALE Tools for Sensitivity and Uncertainty Analysis Methodology Implementation (TSUNAMI) sequences were then used to compare the neutronic characteristics of 1584 International Criticality Safety Benchmark Evaluation Project benchmark critical experiments with the NCT and HAC HALEU transportation models. The TSUNAMI integral correlation coefficient c k was the criterion used to rank neutronic similarity. Thirty-four experiments were identified as similar (c k ≥ 0.9) to the NCT model, and 55 experiments were identified as similar to the HAC model. Hundreds of experiments were also identified as at least marginally similar (c k ≥ 0.8) to both models. The results indicate that additional critical experiments are unlikely to be needed to support HALEU transportation criticality safety analyses for package concepts similar to the concept package analyzed.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Characterizing Microfluidic Operations Underlying an Electrowetting Heat Pipe on the International Space Station

Electrowetting heat pipes (EHPs) are a newly conceptualized class of heat pipes, wherein the adiabatic wick section is replaced by electrowetting-based pumping of the condensate (as droplets) to the evaporator. Specific advantages include the ability to transport high heat loads over long distances, low thermal resistance and power consumption, and the absence of moving mechanical parts. In this work, we describe characterization of key microfluidic operations (droplet motion and splitting) underlying the EHP on the International Space Station (ISS). The testing was performed under the Advanced Passive Thermal eXperiment (APTx) project, a project to test a suite of passive thermal control devices funded by the ISS Technology Demonstration Office at NASA JSC (Johnson Space Center). A rapid manufacturing method was used to fabricate the electrowetting device on a printed circuit board. Key device-related considerations were to ensure reliability and package the experimental hardware within a confined space. Onboard the ISS, experiments were conducted to study electrowetting-based droplet motion and droplet splitting, by imaging droplet manipulation operations via pre-programmed electrical actuation sequences. An applied electric field of 36 Volts per micron resulted in droplet speeds approaching 10 millimeters per second. Droplet splitting dynamics were observed and the time required to split droplets was quantified. Droplet motion data was analyzed to estimate the contact line friction coefficient. Overall, this demonstration is the first-ever electrowetting experiment in space. The obtained results are useful for future design of the EHP and other electrowetting-based systems for microgravity applications.

Electrowetting↗

Paragenetic controls on CO 2 -fluid-rock interaction and weakening in a macroporous-dominated sandstone

The injection and storage of anthropogenic CO 2 in the subsurface is being deployed as a climate change mitigation tool; however, diagenetic-paragenetic heterogeneity in sandstone reservoirs often contributes to interval specific chemomechanical changes that affect injection and can increase leakage risk.In this paper we address reservoir heterogeneities’ impact on chemomechanical changes in a macroporous-dominated lithofacies of Morrow B sandstone, a formation containing several diagenetically-distinct hydraulic facies while undergoing enhanced oil recovery (EOR) and carbon dioxide (CO 2 ) sequestration. We performed three flow-through experiments using a CO 2 -charged or uncharged formation water combined with four indirect tensile strength tests per post-test sample. We then used the microstructure and paragenetic sequence to understand chemomechanical weakening with key observations as follows: dissolution of carbonates and feldspars changed porosity; increased permeability led to reclassifying each sample in a different hydraulic flow unit; decreased ultrasonic velocity; and did not lead to a loss of tensile strength. Tensile strength maintenance occurred due to the low abundance and minor dissolution of siderite, the stability of quartz, and the relative position of diagenetic ankerite within feldspar. This macroporous-dominated lithofacies is the primary reservoir for the Morrow B Sandstone, and is analogous to other porous sandstone reservoirs. It represents an end-member of a chemomechanically low-risk siliceous CO 2 sequestration and CO 2 -EOR reservoir.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Intensive agricultural management‐induced subsurface accumulation of labile phosphorus in Midwestern agricultural soils dominated by tile lines

Abstract In addition to surface runoff, subsurface P loss through tile lines in agricultural lands has received attention over the last decade. It is not clearly understood how this strong ligand, phosphate, is distributed in subsoils (≤180 cm), contributing to subsurface P loss. We hypothesized that subsurface accumulation of labile P as a result of long‐term intensive agricultural management contributes to subsurface P loss. The depth sequence distribution of P and its speciation and reactivity were investigated in tile‐line‐dominated Midwestern agricultural fields via chemical extraction and digestion, batch desorption experiments, and X‐ray absorption near‐edge structure spectroscopy. The results suggest that labile P in subsoils exceeds the eutrophication‐inducing level (>0.01 mg L –1 ). Because of intensive agricultural management, total P was ∼400 mg kg –1 in surface soils and ∼200‐300 mg kg –1 in subsoils. The depth sequence distribution of P indicated the translocation of P to subsoils. Although phosphate and phytic acid adsorbed by Al or Fe (oxyhydro)oxides and calcite were found in surface soils, phosphate adsorbed to calcite, hydroxyapatite, or both were the dominant P species in subsoils. Phosphate was highest in surface soils and decreased sharply with increasing depth. Slightly alkaline soil in subsoils suppressed the release of P but the process was continuous after 30 d. Subsurface P loss was influenced by the dissolution and desorption process of inorganic P phases and preferentially transported P from surface soils. Subsurface P loss should be considered in strategies to reduce agricultural P loss in the Gulf of Mexico.

Xu, Suwei↗

A Novel TDC Scheme: Combinatorial Gray Code Oscillator Based TDC for Low Power and Low Resource Usage Applications

In common digital electronics design practice, combinatorial loop is less preferable, especially in the multi-bit cases. An exception of multi-bit feedback system that is allowed to be implemented in combinatorial loop is the Gray code sequence generator. The Gray code sequence generator runs by itself without being driven by an external clock. It steps through Gray code sequence significantly faster than the Gray code counter under the same silicon technology. In high energy physics experiments, it is necessary to find a low resource usage and low power TDC (Time-to-Digital-Converter) scheme for high channel count detectors. A Gray code oscillator based TDC is a good candidate for such a scheme. In this paper, we describe our work of design and implementation of a TDC based on combinatorial Gray code oscillator in a Xilinx Kintex-7 FPGA (Field-Programmable Gate Array). The implementation and test results are presented. The test result shows that the TDC using only 8 logic elements is able to reach a RMS precision of 160 ps for time difference measurements of a single pulse. The single pulse measurement precision can be further improved to 51 ps with the weighted average scheme using data from the same TDC channels captured by 4 consecutive system clock edges.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Distributed Intrusion Detection System using Semantic-based Rules for SCADA in Smart Grid

Cyber-physical system (CPS) security for the smart grid enables secure communication for the SCADA and wide-area measurement system data. Power utilities world-wide use various SCADA protocols, namely DNP3, Modbus, and IEC 61850, for the data exchanges across substation field devices, remote terminal units (RTUs), and control center applications. Adversaries may exploit compromised SCADA protocols for the reconnaissance, data exfiltration, vulnerability assessment, and injection of stealthy cyberattacks to affect power system operation. In this paper, we propose an efficient algorithm to generate robust rule sets. We integrate the rule sets into an intrusion detection system (IDS), which continuously monitors the DNP3 data traffic at a substation network and detects intrusions and anomalies in real-time. To enable CPS-aware wide-area situational awareness, we integrated the methodology into an open-source distributed-IDS (D-IDS) framework. The D-IDS facilitates central monitoring of the detected anomalies from the geographically distributed substations and to the control center. The proposed algorithm provides an optimal solution to detect network intrusions and abnormal behavior. Different types of IDS rules based on packet payload, packet flow, and time threshold are generated. Further, IDS testing and evaluation is performed with a set of rules in different sequences. The detection time is measured for different IDS rules, and the results are plotted. All the experiments are conducted at Power Cyber Lab, Iowa State University, for multiple power grid models. After successful testing and evaluation, knowledge and implementation are transferred to field deployment.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Phylogenetic conservatism of mycoparasitism and its contribution to pathogen antagonism

Abstract Closely related species are expected to have similar functional traits due to shared ancestry and phylogenetic inertia. However, few tests of this hypothesis are available for plant‐associated fungal symbionts. Fungal leaf endophytes occur in all land plants and can protect their host plant from disease by a variety of mechanisms, including by parasitizing pathogens (e.g., mycoparasitism). Here, we tested whether phylogenetic relatedness among species of Cladosporium , a widespread genus that includes mycoparasitic species, predicts the effect of this endophyte on the severity of leaf rust disease. First, we used congruence among different marker sequences (i.e., genealogical concordance phylogenetic species recognition criterion) to delimit species of Cladosporium . Next, in a controlled experiment, we quantified both mycoparasitism and disease modification for the selected Cladosporium species. We identified 17 species of Cladosporium ; all the species reduced rust disease severity in our experiment. Cladosporium phylogeny was a significant predictor of mycoparasitism. However, we did not observe a phylogenetic effect on disease severity overall, indicating that other mechanism/s operating independently of shared ancestry also contributed to endophyte effects on disease severity. Indeed, a second experiment showed that Cladosporium endophyte exudates (no live organism) from divergent species groups equally reduced disease severity. Our results reveal that multiple mechanisms contribute to the protective effects of an endophyte against a plant pathogen, but not all traits underlying these mechanisms are phylogenetically conserved.

Barge, Edward G.↗

Aerial Crosspolarized NQR-NMR: Buried Explosive Detection From a Safe Distance

Nuclear quadrupole resonance is a non-destructive detection and inspection technique with potential as a non-destructive test (NDT) tool. Establishment of the capability opens the door to its use in furthering the mission of the labs. There are many possible uses of the capability: explosive detection and stress/strain detection in epoxies are two of the more obvious and are the main results of this work. Enhancement of the signal-to-noise ratio (SNR) and improvements in the acquisition time of the experiment were key focuses of this work. These were achieved by combing special spin-lock pulse sequences with cross-polarization (CP) schemes to improve the signals with shorter acquisition times. A novel rotating magnetic field device was created to facilitate CP in the field. Implementation of these schemes provided a significant reduction in SNR/time.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗