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At least 469 records · Page 26

Positive correlation between wood δ 15 N and stream nitrate concentrations in two temperate deciduous forests

Abstract A limitation to understanding drivers of long-term trends in terrestrial nitrogen (N) availability in forests and its subsequent influence on stream nitrate export is a general lack of integrated analyses using long-term data on terrestrial and aquatic N cycling at comparable spatial scales. Here we analyze relationships between stream nitrate concentrations and wood δ 15 N records (n = 96 trees) across five neighboring headwater catchments in the Blue Ridge physiographic province and within a single catchment in the Appalachian Plateau physiographic province in the eastern United States. Climatic, acidic deposition, and forest disturbance datasets were developed to elucidate the influence of these factors on terrestrial N availability through time. We hypothesized that spatial and temporal variation of terrestrial N availability, for which tree-ring δ 15 N records serve as a proxy, affects the variation of stream nitrate concentration across space and time. Across space at the Blue Ridge study sites, stream nitrate concentration increased linearly with increasing catchment mean wood δ 15 N. Over time, stream nitrate concentrations decreased with decreasing wood δ 15 N in five of the six catchments. Wood δ 15 N showed a significant negative relationship with disturbance and acidic deposition. Disturbance likely exacerbated N limitation by inducing nitrate leaching and ultimately enhancing vegetative uptake. As observed elsewhere, lower rates of acidic deposition and subsequent deacidification of soils may increase terrestrial N availability. Despite the ephemeral modifications of terrestrial N availability by these two drivers and climate, long-term declines in terrestrial N availability were robust and have likely driven much of the declines in stream nitrate concentration throughout the central Appalachians.

54 ENVIRONMENTAL SCIENCES↗

Sea Level-Driven Marsh Migration Results in Rapid Net Loss of Carbon

Sea level rise alters coastal carbon cycling by driving the rapid migration of coastal ecosystems, salinization of freshwater systems, and replacement of terrestrial forests with tidal wetlands. Wetland soils accumulate carbon (C) at faster rates than terrestrial soils, implying that sea level rise may lead to enhanced carbon accumulation. Here, we show that carbon stored in tree biomass greatly exceeds carbon stored in adjacent marsh soils so that marsh migration reduces total carbon stocks by ~50% in less than 100 years. Continued marsh soil carbon accumulation may eventually offset forest carbon loss, but we estimate that the time for replacement is similar to estimates of marsh survival (i.e., centuries), which suggests that forest C may never be replaced. These findings reveal a critical C source not included in coastal C budgets driven by migrating ecosystems and rapidly shifting allocations between carbon stored in soils and biomass.

58 GEOSCIENCES↗

A minor respiratory process with major global implications: is atmospheric methane oxidation in tree stems driven by stem respiration rather than microbial methanotrophy?

Tree stem surfaces are widely recognized as sites of carbon dioxide (CO₂) efflux and oxygen (O₂) influx, reflecting the dynamics of aerobic respiration of photosynthate substrates, such as sugars, delivered via the phloem. Stems are also largely considered passive conduits for methane (CH₄) produced in anoxic soils via microbial methanogenesis, where CH₄ is thought to be transported upward through the transpiration stream and/or diffusion and emitted through stem surfaces and the canopy. However, recent observations from dynamic stem chambers suggest that stems may also act as active sinks for atmospheric CH₄. Despite these findings, the extent and drivers of stem CH₄ consumption remain poorly characterized across biomes, species, and environmental gradients, and its quantitative relationship to stem respiration has not been established. Moreover, previous studies captured only snapshot fluxes, leaving diurnal patterns of CH₄ exchange uncharacterized. Here, we address these limitations by combining real-time measurements of stem CH₄ and O₂ uptake under ambient conditions in a California cherry tree, using a dynamic stem gas exchange system with three chambers receiving a continuous flow of ambient air and automated chamber and reference air sampling every 10 min. Our results confirm that stems of upland trees can actively consume both atmospheric CH₄ and O₂, but with decreasing temperature sensitivity as daily temperatures increase. Early mornings were marked by rapid influxes of both gases, followed by declining uptake as temperatures rose further. Methane uptake was tightly coupled with O₂ influx and represented a minor (0.012% ± 0.002%) fraction of stem respiratory activity, as determined by concurrent O₂ uptake. These findings suggest that while atmospheric CH₄ oxidation is a minor respiratory process in stems, it is strongly linked with stem physiological activity. This challenges the current assumption that terrestrial CH₄ uptake is driven solely by microbial methanotrophy and raises the possibility that living stem tissues may contribute to CH₄ oxidation through an as-yet-unidentified plant-based mechanism.

Atmospheric greenhouse gases↗

Long-Term Persistence of Three Microbial Wildfire Biomarkers in Forest Soils

Long-term monitoring of microbial communities in the rhizosphere of post-fire forests is currently one of the key knowledge gaps. Knowing the time scale of the effects is indispensable to aiding post-fire recovery in vulnerable woodlands, including holm oak forests, that are subjected to a Mediterranean climate, as is the case with forests that are found in protected areas such as the Sierra Nevada National and Natural Park in southeastern Spain. We took rhizosphere soil samples from burned and unburned holm oak trees approximately 3, 6, and 9 years after the 2005 fire that devastated almost 3500 ha in southeastern Spain. We observed that the prokaryotic communities are recovering but have not yet reached the conditions observed in the unburned forest. A common denominator between this fire and other fires is the long-term persistence of three ecosystem recovery biomarkers—specifically, higher proportions of the genera Arthrobacter, Blastococcus, and Massilia in soil microbial communities after a forest fire. These pyrophilous microbes possess remarkable resilience against adverse conditions, exhibiting traits such as xerotolerance, nitrogen mineralization, degradation of aromatic compounds, and copiotrophy in favorable conditions. Furthermore, these biomarkers thrive in alkaline environments, which persist over the long term following forest fires. The relative abundance of these biomarkers showed a decreasing trend over time, but they were still far from the values of the control condition. In conclusion, a decade does not seem to be enough for the complete recovery of the prokaryotic communities in this Mediterranean ecosystem.

54 ENVIRONMENTAL SCIENCES↗

Seed classification with random forest models

Premise: To improve forest conservation monitoring, we developed a protocol to automatically count and identify the seeds of plant species with minimal resource requirements, making the process more efficient and less dependent on human operators. Methods and Results: Seeds from six North American conifer tree species were separated from leaf litter and imaged on a flatbed scanner. In the most successful species-classification approach, an ImageJ macro automatically extracted measurements for random forest classification in the software R. The method allows for good classification accuracy, and the same process can be used to train the model on other species. Conclusions: This protocol is an adaptable tool for efficient and consistent identification of seed species or potentially other objects. Automated seed classification is efficient and inexpensive, making it a practical solution that enhances the feasibility of large-scale monitoring projects in conservation biology.

59 BASIC BIOLOGICAL SCIENCES↗

Airborne LiDAR to Improve Canopy Fuels Mapping for Wildfire Modeling

Increasing conflict between wildfire and the built environment has increased the need for more up-to-date and finer resolution canopy fuels data to improve wildfire modeling and associated risk forecasts. The US Forest Service and US Department of the Interior’s LANDFIRE product, which provides 30-m resolution canopy fuels data for the entire US, is one of the most widely used sources of fuels data. However, the last complete mapping effort for LANDFIRE is based on 2016 conditions, and subsequent updates reflect disturbances 1-2 years behind the release year. Airborne systems equipped with Light Detection and Ranging (LiDAR) sensors can be deployed to actively sense canopy structure and estimate canopy fuels data (cover, height, base height, bulk density) at finer resolutions. Canopy base height (CBH) and canopy bulk density (CBD) are difficult to measure both in the field and in LiDAR point clouds. Still, they are important for accurately modeling crown fires, which are often intense and difficult to contain. Additionally, point cloud datasets are large, and calculations require efficient utilization of computational resources. To address these challenges, we are working on an approach that uses openly available National Ecological Observatory Network (NEON) airborne LiDAR data, with calculations processed in the R programming language and parallelized through the lidR package. CBH and CBD are often derived from tree height, diameter at breast height, and species-specific allometries using the Fire and Fuels Extension of the Forest Vegetation Simulator (FFE-FVS). We aim to test if airborne LiDAR can estimate CBH and CBD without the use of empirical equations. Reliable estimates of canopy fuels data directly from airborne LiDAR could streamline quick, fine-resolution updates for use in wildfire behavior models.

54 ENVIRONMENTAL SCIENCES↗

Prevalent root-derived phenolics drive shifts in microbial community composition and prime decomposition in forest soil

Phenolic compounds perform various functions in soil ranging from microbial substrate to toxin and form the basis of several plant-mediated processes. The aim of this study was to investigate how phenolics commonly exuded by tree roots influence soil organic matter (SOM) decomposition and interact with other labile forms of carbon (C) abundant in root exudates. Therefore, we performed a 38-day incubation experiment and assessed whether phenolic compounds (benzoic acid, caffeic acid and catechin) facilitated or inhibited SOM decomposition in a glucose-amended forest soil. Changes in decomposition, substrate use, fungal and bacterial community composition, and microbial abundance and activity were measured over time using 13 C-stable-isotope tracing, DNA-based molecular methods and enzyme assays. Our findings showed that phenolics inhibited microbial activity and abundance to varying degrees. Yet, benzoic acid was the only compound producing a substantial priming effect leading to a 21% increase in SOM decomposition, which was amplified in glucose-amended soils. This stimulation in microbial activity was associated with an increase in β-1,4-glucosidase activity and the bacterial genera Paraburkholderia and Caballeronia of the Burkholderiaceae family. Phenolics drove microbial community shifts in glucose-amended soils with negligible interactive effects. In conclusion, phenolic priming of SOM decomposition is associated with microbial community shifts and amplified in the presence of glucose. This evidence emphasizes the need for considering phenolics and interactions among root exudates as priming mechanisms in the rhizosphere and other soil environments where aromatics and phenolic compounds are abundant.

59 BASIC BIOLOGICAL SCIENCES↗

Mycorrhiza better predict soil fungal community composition and function than aboveground traits in temperate forest ecosystems

We sampled soil in the organic and mineral horizons beneath two AM-associated (Fraxinus americana, Thuja occidentalis) and two ECM-associated tree species (Betula alleghaniensis, and Tsuga canadensis), with an evergreen and deciduous species in each mycorrhizal group. To characterize fungal communities and organic matter decomposition beneath each tree species, we sequenced the ITS1 region of fungal DNA and measured the potential activity of carbon and nitrogen-targeting extracellular enzymes. Each tree species harbored distinct fungal communities, supporting the need to consider both mycorrhizal type and leaf habit. However, between tree characteristics, mycorrhizal type better predicted fungal communities. Across fungal guilds, saprotrophic fungi were the most important group in shaping fungal community differences in soils beneath all tree species. The effect of leaf habit on carbon and nitrogen-targeting hydrolytic enzymes depended on tree mycorrhizal association in the organic horizon, while oxidative enzyme activities were higher beneath EcM-associated trees across both soil horizons and leaf habits. These data include extracellular hydrolytic and oxidative enzyme activities, ITS sequencing fungal community data, soil carbon to nitrogen, and soil pH. Site level data include climate (mean annual temperature, precipitation), elevation, and soil series and order information.

54 ENVIRONMENTAL SCIENCES↗

Vehicle Position Detection Based on Machine Learning Algorithms in Dynamic Wireless Charging

Dynamic wireless charging (DWC) has emerged as a viable approach to mitigate range anxiety by ensuring continuous and uninterrupted charging for electric vehicles in motion. DWC systems rely on the length of the transmitter, which can be categorized into long-track transmitters and segmented coil arrays. The segmented coil array, favored for its heightened efficiency and reduced electromagnetic interference, stands out as the preferred option. However, in such DWC systems, the need arises to detect the vehicle’s position, specifically to activate the transmitter coils aligned with the receiver pad and de-energize uncoupled transmitter coils. This paper introduces various machine learning algorithms for precise vehicle position determination, accommodating diverse ground clearances of electric vehicles and various speeds. Through testing eight different machine learning algorithms and comparing the results, the random forest algorithm emerged as superior, displaying the lowest error in predicting the actual position.

47 OTHER INSTRUMENTATION↗

Hydraulic redistribution supplies a major water subsidy and improves water status of understory species in a longleaf pine ecosystem

Hydraulic redistribution (HR) is a common phenomenon in water-limited ecosystems; however, it remains unclear how the volume of water transported via HR compares to other components of the hydrologic budget and how HR influences water availability for understory plant communities. In this study, we investigate the absolute and relative magnitude of HR on a forest water budget and identify potential impacts of this water subsidy to understory plant communities. We scaled tree-level estimates of transpiration and HR of three common tree species naturally occurring in a longleaf pine woodland with plot-level measurements of basal area to determine their magnitude at the stand scale. We trenched plots containing understory vegetation but devoid of mature trees and their connected roots to exclude HR subsidies to understory plant species. We analysed soil water isotopes and assessed leaf water potential (Ψ L ) in trenched and control plots to determine if HR results in mixing of water among soil strata and improves understory plant moisture status. Water inputs from HR were equivalent to >30% of total rainfall for the site during the observation period and ~40% of total tree water uptake, depending on species. A stable isotope mixing model confirmed that soil water within HR-exposed plots was more similar to groundwater, whereas soil water within trenched plots was more similar to precipitation. Exclusion of HR via trenching decreased soil moisture and pre-dawn Ψ L for all understory species. These three lines of evidence suggest that HR from overstory trees redistributes a sizable portion of water from deeper to shallower soil profiles and that this water subsidy enhances understory plant water status.

54 ENVIRONMENTAL SCIENCES↗

The functional roles of species in metacommunities, as revealed by metanetwork analyses of bird–plant frugivory networks

Abstract Understanding how biodiversity and interaction networks change across environmental gradients is a major challenge in ecology. We integrated metacommunity and metanetwork perspectives to test species’ functional roles in bird–plant frugivory interactions in a fragmented forest landscape in Southwest China, with consequences for seed dispersal. Availability of fruit resources both on and under trees created vertical feeding stratification for frugivorous birds. Bird–plant interactions involving birds feeding only on‐the‐tree or both on and under‐the‐tree (shared) had a higher centrality and contributed more to metanetwork organisation than interactions involving birds feeding only under‐the‐tree. Moreover, bird–plant interactions associated with large‐seeded plants disproportionately contributed to metanetwork organisation and centrality. Consequently, on‐the‐tree and shared birds contributed more to metanetwork organisation whereas under‐the‐tree birds were more involved in local processes. We would expect that species’ roles in the metanetwork will translate into different conservation values for maintaining functioning of seed‐dispersal networks.

Li, Hai‐Dong↗

AmeriFlux FLUXNET-1F US-Me5 Metolius-first young aged pine

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Me5 Metolius-first young aged pine. This is the FLUXNET version of the carbon flux data for the site US-Me5 Metolius-first young aged pine produced by applying the standard ONEFlux (1F) software. Site Description - Previously old-growth ponderosa pine, clearcut in 1978 and allowed to regenerate naturally. Law et al (2001) Global Change Biology 7, 755-777; Law et al (2001) Agricultural and Forest Meteorology 110, 27-43; Anthoni et al (2002) Agricultural and Forest Meteorology 111, 203-222; Irvine & Law (2002) Global Change biology 8,1183-1194, Irivne et al (2004) Tree Physiology 24,753-763.

Law, Bev [Oregon State University]↗

Modeling the Joint Effects of Vegetation Characteristics and Soil Properties on Ecosystem Dynamics in a Panama Tropical Forest

Abstract In tropical forests, both vegetation characteristics and soil properties are important not only for controlling energy, water, and gas exchanges directly but also determining the competition among species, successional dynamics, forest structure and composition. However, the joint effects of the two factors have received limited attention in Earth system model development. Here we use a vegetation demographic model, the Functionally Assembled Terrestrial Ecosystem Simulator (FATES) implemented in the Energy Exascale Earth System Model (E3SM) Land Model (ELM), ELM‐FATES, to explore how plant traits and soil properties affect tropical forest growth and composition concurrently. A large ensemble of simulations with perturbed vegetation and soil hydrological parameters is conducted at the Barro Colorado Island, Panama. The simulations are compared against observed carbon, energy, and water fluxes. We find that soil hydrological parameters, particularly the scaling exponent of the soil retention curve ( B sw ), play crucial roles in controlling forest diversity, with higher B sw values (>7) favoring late successional species in competition, and lower B sw values (1 ∼ 7) promoting the coexistence of early and late successional plants. Considering the additional impact of soil properties resolves a systematic bias of FATES in simulating sensible/latent heat partitioning with repercussion on water budget and plant coexistence. A greater fraction of deeper tree roots can help maintain the dry‐season soil moisture and plant gas exchange. As soil properties are as important as vegetation parameters in predicting tropical forest dynamics, more efforts are needed to improve parameterizations of soil functions and belowground processes and their interactions with aboveground vegetation dynamics.

54 ENVIRONMENTAL SCIENCES↗

Random Forest Regressor-Based Approach for Detecting Fault Location and Duration in Power Systems

Power system failures or outages due to short-circuits or “faults” can result in long service interruptions leading to significant socio-economic consequences. It is critical for electrical utilities to quickly ascertain fault characteristics, including location, type, and duration, to reduce the service time of an outage. Existing fault detection mechanisms (relays and digital fault recorders) are slow to communicate the fault characteristics upstream to the substations and control centers for action to be taken quickly. Fortunately, due to availability of high-resolution phasor measurement units (PMUs), more event-driven solutions can be captured in real time. In this paper, we propose a data-driven approach for determining fault characteristics using samples of fault trajectories. A random forest regressor (RFR)-based model is used to detect real-time fault location and its duration simultaneously. This model is based on combining multiple uncorrelated trees with state-of-the-art boosting and aggregating techniques in order to obtain robust generalizations and greater accuracy without overfitting or underfitting. Four cases were studied to evaluate the performance of RFR: 1. Detecting fault location (case 1), 2. Predicting fault duration (case 2), 3. Handling missing data (case 3), and 4. Identifying fault location and length in a real-time streaming environment (case 4). A comparative analysis was conducted between the RFR algorithm and state-of-the-art models, including deep neural network, Hoeffding tree, neural network, support vector machine, decision tree, naive Bayesian, and K-nearest neighborhood. Experiments revealed that RFR consistently outperformed the other models in detection accuracy, prediction error, and processing time.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Benchmarking and parameter sensitivity of physiological and vegetation dynamics using the Functionally Assembled Terrestrial Ecosystem Simulator (FATES) at Barro Colorado Island, Panama

Plant functional traits determine vegetation responses to environmental variation, but variation in trait values is large, even within a single site. Likewise, uncertainty in how these traits map to Earth system feedbacks is large. We use a vegetation demographic model (VDM), the Functionally Assembled Terrestrial Ecosystem Simulator (FATES), to explore parameter sensitivity of model predictions, and comparison to observations, at a tropical forest site: Barro Colorado Island in Panama. We define a single 12-dimensional distribution of plant trait variation, derived primarily from observations in Panama, and define plant functional types (PFTs) as random draws from this distribution. We compare several model ensembles, where individual ensemble members vary only in the plant traits that define PFTs, and separate ensembles differ from each other based on either model structural assumptions or non-trait, ecosystem-level parameters, which include (a) the number of competing PFTs present in any simulation and (b) parameters that govern disturbance and height-based light competition. While single-PFT simulations are roughly consistent with observations of productivity at Barro Colorado Island, increasing the number of competing PFTs strongly shifts model predictions towards higher productivity and biomass forests. Different ecosystem variables show greater sensitivity than others to the number of competing PFTs, with the predictions that are most dominated by large trees, such as biomass, being the most sensitive. Changing disturbance and height-sorting parameters, i.e., the rules of competitive trait filtering, shifts regimes of dominance or coexistence between early- and late-successional PFTs in the model. Increases to the extent or severity of disturbance, or to the degree of determinism in height-based light competition, all act to shift the community towards early-successional PFTs. In turn, these shifts in competitive outcomes alter predictions of ecosystem states and fluxes, with more early-successional-dominated forests having lower biomass. It is thus crucial to differentiate between plant traits, which are under competitive pressure in VDMs, from those model parameters that are not and to better understand the relationships between these two types of model parameters to quantify sources of uncertainty in VDMs.

54 ENVIRONMENTAL SCIENCES↗

Decoding the chemical language of Suillus fungi: genome mining and untargeted metabolomics uncover terpene chemical diversity

ABSTRACT Ectomycorrhizal fungi establish mutually beneficial relationships with trees, trading nutrients for carbon. Suillus are ectomycorrhizal fungi that are critical to the health of boreal and temperate forest ecosystems. Comparative genomics has identified a high number of non-ribosomal peptide synthetase and terpene biosynthetic gene clusters (BGC) potentially involved in fungal competition and communication. However, the functionality of these BGCs is not known. This study employed co-culture techniques to activate BGC expression and then used metabolomics to investigate the diversity of metabolic products produced by three Suillus species ( Suillus hirtellus EM16, Suillus decipiens EM49, and Suillus cothurnatus VC1858), core members of the pine microbiome. After 28 days of growth on solid media, liquid chromatography–tandem mass spectrometry identified a diverse range of extracellular metabolites (exometabolites) along the interaction zone between Suillus co-cultures. Prenol lipids were among the most abundant chemical classes. Out of the 62 unique terpene BGCs predicted by genome mining, 41 putative prenol lipids (includes 37 putative terpenes) were identified across the three Suillus species using metabolomics. Notably, some terpenes were significantly more abundant in co-culture conditions. For example, we identified a metabolite matching to isomers isopimaric acid, sandaracopimaric acid, and abietic acid, which can be found in pine resin and play important roles in host defense mechanisms and Suillus spore germination. This research highlights the importance of combining genomics and metabolomics to advance our understanding of the chemical diversity underpinning fungal signaling and communication. IMPORTANCE Using a combination of genomics and metabolomics, this study’s findings offer new insights into the chemical diversity of Suillus fungi, which serve a critical role in forest ecosystems.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Growing Insulation in Alaska

Buildings produce 40% of all carbon emissions in the U.S. This includes the energy needed to heat, cool, and power the nation's buildings and the energy used to manufacture, ship, and assemble the construction materials. In extreme climates and remote regions, buildings use even more energy, as building materials must be produced and shipped farther to the site, increasing the greenhouse gas emissions embodied in the buildings. The U.S. National Renewable Energy Laboratory (NREL) is developing technologies that reduce how much energy our buildings use and how much carbon goes into them in the first place. NREL researchers teamed up with the Biomaterials Laboratory at the University of Alaska, U.S. Forest Service Forest Products Lab, and the VTT Centre of Finland on a three-year project to "grow" insulation using trees in Alaska - improving the efficiency of buildings by providing envelope retrofit options using local resources.

carbon negative building↗

Collaborative Research On Ecophysiological Controls on Amazonian Precipitation Seasonality And Variability

We developed and deployed a robust and accurate instrumentation package for measurement of solar induced chlorophyll fluorescence (SIF) from above a plant canopy. The sensor collects light from identical up and down facing hemispheric collectors. A fiber optic transmits the collected light (selected by a rotatable prism) to spectrometers located at the base of the tower. Spectra of the down welling and upwelling light were collected alternatively and stored for later analysis. SIF was retrieved by a spectral unmixing approach using high resolution spectra in the 730-760 nm band. Most other ground-based sensors base their analysis on O 2 -alpha band (760-780 nm) for SIF retrieval. We found that the former gave identical results when the atmosphere was clear, but the former was more stable in the cloudy conditions that often prevailed in the Amazon. The mechanical design and software developed for the system are available on request. The sensor was mounted near the top of an 80 m tower at K34 near Manaus in the Brazilian Amazon Forest. The sensor system proved weather-tight and functioned flawlessly over the time of deployment. Our experiment was cut short at 6 months by failure of air conditioning infrastructure at the tower. We found that SIF emitted by canopy was remarkably stable when scaled by the downwelling photosynthetically active radiation or the upwelling near infrared light. We detected a 3.4% increase in SIF over the course of the dry season. This finding is consistent with the observation that several trees in the field of view flushed new leaves in the dry season. We saw no evidence of drought stress or photo damage of the forest canopy during our study.

54 ENVIRONMENTAL SCIENCES↗