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At least 469 records · Page 26

Data and scripts associated with the manuscript evaluating the hydrologic responses of the Pacific Northwest watersheds to wildfires (v2)

This data package is associated with the publication “Evaluating Post-fire Watershed Response to Varying Burn Severity and Precipitation Regimes Using Fully-distributed and Integrated Hydrologic Models” submitted to Journal of Hydrology (Li et al. 2025). In this study, we employed the Advanced Terrestrial Simulator (ATS), an integrated watershed model that couples surface flow, subsurface flow, and canopy biophysical processes, to investigate post-fire hydrologic responses in a few selected watersheds with varying burn severity.The data package contains the required input data (meteorological forcing, Leaf Area Index, wildfire burn severities, etc.) to run the model, configuration files, the Jupyter notebooks in Python to pre-process and post-process data, the figures in the manuscript, and the modeling output files. The variables include watershed-averaged evapotranspiration, watershed-averaged surface/subsurface/canopy water content, and river discharge at watershed outlet.The data package contains a file-level metadata that lists and describes all the files contained in the data package (ATS_flmd.csv), a data dictionary file that defines columns headers across all csv files contained in the data package (ATS_dd.csv), a data package level readme file (the current file), and four zipped folders.The ‘data’ folder provides data needed to run the model in .h5, .i2s, .xyz, .shp, and .exo formats. The sub-folders are for each data types. The ‘model’ folder provides input files (.xml format) and essential model outputs. Each sub-folder provides the files from each simulated watershed. The ‘notebooks’ folder provides the Jupyter notebooks (.ipynb format) for pre- and post- processing model files, and for producing the figures in the manuscript. The ‘figures’ folder provides the figures associated with manuscript in .pdf and .png formats.The ‘model’ folder and the ‘data’ folder have been split into 5GB-large pieces using the Linux command ‘split -b 5120m model.zip model.zip.’ and ‘split -b 5120m data.zip data.zip.’, respectively. They can be merged back using the Linux command ‘cat model.zip.* > model.zip’ and ‘cat data.zip.* > data.zip’, respectively.

54 ENVIRONMENTAL SCIENCES↗

Improving the Estimation of the Atmospheric Water Vapor Pressure Using Interpretable Long Short-Term Memory Networks: Dataset, Python code, and trained models

Atmospheric water vapor pressure is an essential meteorological control on land surface and hydrologic processes. It is not as frequently observed as other meteorologic conditions, but often inferred through the August–Roche–Magnus formula by simply assuming dew point and daily minimum temperatures are equivalent or by empirically correlating the two temperatures using an aridity correction. The performance of both methods varies considerably across different regions and during different time periods; obtaining consistently accurate estimates across space and time remains a great challenge. We applied an interpretable Long Short-Term Memory (iLSTM) network conditioned on static, location specific attributes to estimate daily vapor pressure for 83 FLUXNET sites in the United States and Canada. This data package includes all raw data of the 83 FLUXNET sites, input data for model training/validation/test, trained models and results, and python codes for the manuscript "Improving the Estimation of the Atmospheric Water Vapor Pressure Using an Interpretable Long Short-term Memory Network". Specifically, it consists of five parts. - First, "1_Daymet_data_83sites.zip" includes raw data downloaded from Daymet for the 83 sites used in the paper according to their longitude and latitude, in which vapor pressure is used. It also includes a pre-processed CSV data file combining all data from the 83 sites which is specifically used for the paper. - Second, "2_Fluxnet2015_data_83sites.zip" includes raw half hourly data of the 83 sites downloaded from FLUXNET2015 data portal, pre-processed daily data of the 83 sites, a CSV file including combined pre-processed daily data of the 83 sites, and a CSV file including the information (site ID, site name, latitude, longitude, data available period) of the 83 sites. - Third, "3_MODIS_LAI_data_83sites_raw.zip" includes raw leaf area index (LAI) data downloaded from the AppEEARs data portal. - Fourth, "4_Scripts.zip" includes all scripts related to model training and post-processing of a trained model, and a jupyter notebook showing an example for model post-processing. Two typo errors in files titled "run2get_args.py" and "postprocess.py" were corrected on March 27, 2024 to avoid confusions. - Finally, "Trained_models_and_results.zip" includes three folders and three files with suffix ".npy", and each folder corresponds to one file with suffix ".npy" with the same title. Each of the three folders include all trained models associated with one iLSTM model configuration (35 models for each configuration, details are described in the paper). Each file with suffix ".npy" includes the post-processed results of the corresponding 35 models under one iLSTM model configuration.

54 ENVIRONMENTAL SCIENCES↗

Evapotranspiration partitioning estimates from 8 methods from 47 NEON sites, 2019-2021

This dataset provides daily estimates of evapotranspiration (ET) and the transpiration-to-evapotranspiration ratio (T/ET) across 47 terrestrial National Ecological Observatory Network (NEON) sites spanning diverse environmental and biome conditions in the United States across three years of data (2019-2021). Daily ET is reported in both energy units (MJ m⁻² day⁻¹) and equivalent water depth (mm day⁻¹), assuming a constant latent heat of vaporization of 2.45 MJ/kg. The primary method uses a hybrid recurrent neural network–Penman–Monteith framework (RNN-PM), which integrates physically based surface energy balance constraints with data-driven learning to partition ET into transpiration and evaporation components. Model inputs include in situ meteorological observations (air temperature, vapor pressure deficit, wind speed, and radiation) combined with satellite-derived land surface temperature, leaf area index, and soil moisture. For benchmarking and uncertainty assessment, T/ET estimates from seven additional models are included: Priestley-Taylor Jet Propulsion Laboratory (PT-JPL), Penman-Monteith (P-M), Two-Source Energy Balance (TSEB), Support Vector Regression (SVR), and Categorical Boosting (CatBoost), among others—spanning empirical, machine-learning, and process-based approaches (see methods section or linked publication for detailed descriptions). Data Package Contents: The dataset a csv files containing daily ET and T/ET estimates for each site and model, along with associated metadata files these variables. Data can be accessed using common spreadsheet software (e.g., Microsoft Excel, LibreOffice) or programming environments such as R or Python. Together, these data support cross-site comparisons of ecosystem water use, evaluation of ET partitioning methods, and development of improved land–atmosphere exchange models.

EARTH SCIENCE > ATMOSPHERE↗

CHESS 2025: Location data for field observations and sampling

This dataset represents geolocation data associated with field observations and sampling from the Colorado Headwaters Ecological Spectroscopy Study (CHESS) during June and July of 2025. Location data were collected using Trimble DA2 Global Navigation Satellite System (GNSS) receivers with Trimble Catalyst 2 centimeter (cm) positioning service and the Environmental Systems Research Institute (Esri) Field Maps mobile app. Files in this data package include meadow site polygons, shrub site polygons, tree site polygons and stem point locations, and Leaf Area Index (LAI) plot polygons (.geojson). The geojson files can be opened with open-source GIS software (e.g, QGIS). A csv file is also provided with point coordinates for all locations. CHESS Project Description: The Colorado Headwaters Ecological Spectroscopy Study (CHESS) comprised a multi-week airborne remote sensing and field observation campaign in the Upper Gunnison Basin, Colorado, conducted in June and July of 2025. Airborne remote sensing was conducted by the National Ecological Observatory Network Airborne Observation Platform (NEON AOP), concurrent with a field campaign run by the Rocky Mountain Biological Laboratory (RMBL), the Lawrence Berkeley National Laboratory (LBNL) and SLAC National Accelerator Laboratory Watershed Function Science Focus Area (SFA), and NASA-JPL (Jet Propulsion Laboratory) Earth Surface Mineral Dust Source Investigation (EMIT) program. Between June 10 and July 18, 2025, the NEON AOP flight team collected high-resolution aerial imaging spectroscopy and Light Detection and Ranging (LiDAR) data over three domains: the Upper East River (CRBU), Almont Triangle (ALMO), and the Upper Taylor Basin (UPTA). In coordination with the flights, a field campaign acquired ground-truth observations, including observations of vegetation composition, foliar traits, forest demography, and subsurface properties in 18 core sampling areas within the domains. Additional surface water observations were taken at over 380 point locations. All CHESS campaign datasets can be found within the CHESS ESS-DIVE data portal: https://data.ess-dive.lbl.gov/portals/chess. Funding Acknowledgment: Field and remote-sensing data acquisition was performed under a grant from the National Aeronautics and Space Administration (80NSSC24K1005). This work was also supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS Campaigns↗

Photosynthetic parameters and nutrient content of trees at the Panama crane sites.

This data package contains leaf chemistry and photosynthetic traits data from trees within the NGEE Tropics Parque Natural Metropolitano (PA-PNM) and Bosque Protector San Lorenzo (PA-SLZ) sites in Panama. Photosynthetic parameters including maximum photosynthesis, Vcmax, Jmax, and TPU, were calculated from A/Ci curves. Foliar nitrogen and phosphorus concentrations and leaf mass per unit leaf area of the leaves also were determined. The plants that were sampled were classified as to species, family, order, clade, and growth form (tree, liana, perennial herb, or other). Wood density of the tree and the source of that data also are included. Included in this data package is a zip file with a single Excel data file and CSV copies.

54 ENVIRONMENTAL SCIENCES↗

Root responses to warming and hurricane disturbances in a wet tropical forest of Puerto Rico: R code and data

The purpose of this data was to generate a scientific article that describes the responses of tropical roots to a warming experiment and to the effect of two consecutive hurricanes in Puerto Rico (Yaffar et al. in review). This data is from 10 months of minirhizotron images taken every 2 weeks at the experimental warming Tropical Responses to Altered Climate Experiment (TRACE) plots in Puerto Rico before and after Hurricanes Irma and Maria. This project has 3 warmed plots (plot 2,4,6) and 3 control plots (plot 1,3,5) with 2 minirhizotron tubes at each plot. As part of the data, there is also root data taken from cores and in-growth cores. Additionally, there is soil nutrient concentration data, soil microclimate, total leaf area, and canopy openness taken by Reed et al. 2020, and the TRACE census. Data files are in CSV format and the R code included in this package can be used with R 3.4.4 (R Core Team and contributors worldwide).

54 ENVIRONMENTAL SCIENCES↗

Leaf spectral reflectance and transmittance, San Lorenzo, Panama, 2020

This data package contains full-range (350 – 2500 nm) leaf reflectance and transmittance spectra of leaves collected at the San Lorenzo forest canopy crane site in Panama (PA-SLZ) from January to March 2020. Around 1000 leaves were measured from 50 species and various leaf phenological stages. Leaves were sampled from both the top of the canopy and multiple heights within the canopy from 10 vertical profiles and for six core species, from the top of the canopy. This data package includes processed leaf spectra (*.csv), and raw data from the spectroradiometer (compressed as .zip). Metadata files include data descriptions (_dd.csv) for tabular data and sample information and a description of the field campaign protocol (PA-SLZ_2020_Protocol.pdf). In addition to the leaf spectral measurements included here, these samples were also used for measurement of leaf gas exchange, carbon and nitrogen content and leaf mass per unit leaf area (LMA) contained in related data packages.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux FLUXNET-1F US-Me2 Metolius mature ponderosa pine

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Me2 Metolius mature ponderosa pine. This is the FLUXNET version of the carbon flux data for the site US-Me2 Metolius mature ponderosa pine produced by applying the standard ONEFlux (1F) software. Site Description - Site Description before Fire (January, 2002 - August, 2020): The mean stand age is 71 years old and the stand age of the oldest 10% of trees is about 108 years old. This site is one of the Metolius core cluster sites with different age and disturbance classes and part of the AmeriFlux network. The overstory is almost exclusively composed of ponderosa pine trees (Pinus ponderosa Doug. Ex P. Laws) with a few scattered incense cedars (Calocedrus decurrens (Torr.) Florin) and has a peak leaf area index (LAI) of 2.1 m2 m-2. Tree height is relatively homogeneous at about 18 m, and the mean tree density is approximately 339 trees ha-1 (Irvine et al., 2008). The understory is sparse with an LAI of 0.2 m2 m-2 and primarily composed of bitterbrush (Purshia tridentata (Push) DC.) and greenleaf manzanita (Arctostaphylos patula Greene). Soils at the site are sandy (69%/24%/7% sand/silt/clay at 0–0.2 m depth and 66%/27%/7% at 0.2–0.5 m depth, and 54%/ 35%/11% at 0.5–1.0 m depth), freely draining with a soil depth of approximately 1.5 m (Irvine et al., 2008; Law et al., 2001b; Schwarz et al., 2004). Green Ridge Fire: On August 20, 2020, the Green Ridge Fire burned through Us-Me2. The fire was ignited by lightning on August 16th, and grew rapidly to the east over the first few days driven by strong, downslope (westerly) afternoon winds. Fire behavior and observed fire effects were highly heterogeneous due to the localized wind pattern carrying the flaming head of the fire forward, and the efforts being made by suppression resources to contain the fire. The site experienced the full range of fire effects, from <1 m high surface fire that charred litter and duff and only consumed shrubs and herbaceous material to full tree (>15 m) crown fire that consumed 100% of needles, small limbs, and surface fuels at high intensity, leaving only ash and bare soil post-fire. Salvage Logging: From late March to late April 2021, salvage logging by the landowner occurred at the site. Almost all trees within the flux footprint were logged except a small area with lower burn severity, where sap flow and automatic soil respiration measurements are continued since the fire. In August 2022 the primary flux system was moved from the top of the damaged tall tower to a nearby shorter tower centering on the salvage/regenerating footprint.

Hanson, Chad↗

AmeriFlux FLUXNET-1F US-Bar Bartlett Experimental Forest

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Bar Bartlett Experimental Forest. This is the FLUXNET version of the carbon flux data for the site US-Bar Bartlett Experimental Forest produced by applying the standard ONEFlux (1F) software. Site Description - The Bartlett Experimental Forest (448170 N, 71830 W) is located within the White Mountains National Forest in north-central New Hampshire, USA. The 1050 ha forest extends across an elevational range from 200 to 900 m a.s.l. It was established in 1931 and is managed by the USDA Forest Service Northeastern Research Station in Durham, NH. The climate is humid continental with short, cool summers (mean July temperature, 19.8C) and long, cold winters (mean January temperature, 9.8C). Annual precipitation averages 130 cm and is distributed evenly throughout the year. Soils are developed from glacial till and are predominantly shallow, well-drained spodosols. At lowto mid-elevation, vegetation is dominated by northern hardwoods (American beech, Fagus grandifolia; sugar maple, Acer saccharum; yellow birch, Betula alleghaniensis; with some red maple, Acer rubrum and paper birch, Betula papyrifera). Conifers (eastern hemlock, Tsuga canadensis; eastern white pine, Pinus strobus; red spruce, Picea rubens) are occasionally found intermixed with the more abundant deciduous species but are generally confined to the highest (red spruce) and lowest (hemlock and pine) elevations. In 2003, the site was adopted as a NASA North American Carbon Program (NACP) Tier-2 field research and validation site. A 26.5 m high tower was installed in a low-elevation northern hardwood stand in November, 2003, for the purpose of making eddy covariance measurements of the forest–atmosphere exchange of CO2, H2O and radiant energy. Continuous flux and meteorological measurements began in January, 2004, and are ongoing. Average canopy height in the vicinity of the tower is approximately 20–22 m. In the tower footprint, the forest is predominantly classified into red maple, sugar maple, and American beech forest types. Leaf area index in the vicinity of the tower is 3.6 as measured by seasonal litterfall collection, and 4.5 as measured by the optically based Li-Cor LAI-2000 instrument. Further site information: http://www.fs.fed.us/ne/durham/4155/bartlett.htm

Richardson, Andrew↗

AmeriFlux FLUXNET-1F US-Dix Fort Dix

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Dix Fort Dix. This is the FLUXNET version of the carbon flux data for the site US-Dix Fort Dix produced by applying the standard ONEFlux (1F) software. Site Description - The Fort Dix site is located in the upland forests of the New Jersey Pine Barrens, the largest continuous forested landscape on the Northeastern coastal plain. Upland forests occupy 62% of the 1.1 million acre Pine Barrens and can be divided into three dominant stand types, Oak/Pine (19.1%), Pine/Oak (13.1%), and Pitch Pine/Scrub oak (14.3%). The majority of mature upland forests are the product of regeneration following late 19th century logging and charcoaling activities. Gypsy moths first appeared in the Pine Barrens of New Jersey in 1966. Since the time of arrival, the upland forest stands have undergone several episodes of defoliation, the most significant occurred in 1972, 1981, and 1990. In recent years, the overstory oaks and understory oaks and shrubs of the Fort Dix stand, underwent two periods of defoliation by Gypsy moth, in 2006 and 2007. During these two years, maximum leaf area reached only 70% of the 2005 summer maximum.

Clark, Kenneth↗

Evaluation of the Large-Scale and Regional Climatic Response Across North Africa to Natural Variability in Oceanic Modes and Terrestrial Vegetation Among the CMIP5 Models

Hydrologic variability is a serious threat to the poverty-stricken regions of North Africa. Meanwhile, the scientific community struggles to attribute these extreme climatic episodes to specific oceanic and land drivers. Prior modeling studies have not assessed simulated feedbacks over North Africa against an established observational benchmark, so their results are considered as untested, model-specific findings. The Coupled Model Intercomparison Project Phase Five (CMIP5) archive represents the state-of-the-art in climate projections, with most CMIP5 models now containing interactive vegetation phenology; however, there have been no studies to date of their simulated vegetation feedbacks. The capability of CMIP5 models at accurately simulating the forcing of both sea-surface temperature (SST) and regional leaf area index (LAI) anomalies on North African climate needs to be a key consideration in assessing the models’ overall credibility and determining appropriate model weighting for developing climate projections. The Generalized Equilibrium Feedback Assessment (GEFA) is a promising statistical method, which can be applied to either model output or observations, for isolating the local and remote impacts of individual oceanic or terrestrial forcings on regional climate. We performed a combined observational and modeling assessment of land-ocean-atmosphere interactions across the distinct ecological and moisture gradients of North Africa. We evaluated and demonstrated the reliability of the GEFA statistical method over North Africa using the Community Earth System Model (CESM), applied GEFA to observational data to quantify the observed forcing of ocean basin SST anomalies and regional LAI anomalies on North African climate, evaluated the CMIP5 models’ performance in terms of representing these key observed feedbacks, and formulated CMIP5 feedback performance metrics for weighting North African climate projections. Our study represented the first attempt to separate the observed roles of oceanic and vegetation feedbacks across North Africa, the first systematic assessment and intercomparison of land-ocean-atmosphere feedbacks in CMIP5, and the first exploration of vegetation feedbacks among CMIP5 models. The following work was accomplished. (1) The team generated 14 scientific publications and 30 presentations based on the DOE-funded research. (2) A stepwise version of the GEFA statistical method was developed in which unimportant forcings were dropped to increase the reliability of the results. (3) SGEFA was successfully validated through experiments with CESM in terms of its ability to isolate the atmospheric responses to individual oceanic or land forcings. (4) Observational evidence was revealed for the Sahel’s positive vegetation-rainfall feedback on the seasonal to interannual time scale, and it was attributed to a moisture recycling mechanism rather than an albedo mechanism. (5) An approach with SGEFA was developed in which the individual contributions of soil moisture versus vegetation forcings could be separated, revealing that the former forcing outweighed the latter for sub-Saharan Africa. (6) Tropical ocean temperatures were found to be key regulators of pan-tropical vegetation variability, especially for arid and semi-arid regions, including sub-Saharan Africa. (7) The CMIP5 models largely underestimated the importance of land feedbacks across the Sahel. (8) The general consensus among CMIP5 models indicates, for the late 21st century, a diminished seasonal predictability of sub-Saharan African regional climate and an elevated role of the land surface compared to oceanic drivers in regulating regional climate variability. (9) Land-ocean-atmosphere interactions were demonstrated to be key contributors to the seasonal predictability of African wildfire activity. (10) The El Djouf was determined to be of greater importance than the Bodélé depression in terms of providing trans-Atlantic dust transport to the Americas.

54 ENVIRONMENTAL SCIENCES↗

The Consortium for Advanced Sorghum Phenomics (CASP). Final report

The goal of CASP was to accelerate breeding of biomass sorghum [Sorghum bicolor (L.) Moench] by identifying genotypes exhibiting high yield under well-watered, pre- or post-drought and/or salinity-stress conditions. We did this by combining high-throughput, non-invasive drone phenotyping with genomics and molecular profiling. Field-based phenotyping utilized a multi-modal sensor suite of LiDAR, multispectral cameras, and thermal cameras mounted on a commercial drone to detect traits required for yield prediction and selection of drought and saline tolerant lines of sorghum. Traits of interest included plant height (PH), leaf area index (LAI), wet biomass (BMW), and biomass at 65% moisture (BM65) and were measured from emergence to harvest on a weekly basis over three growing seasons. The final output were measurements of traits on a plot-by-plot basis, identified by the plot ID used by the Proprietary data processing software enabled raw field data to be turned into plant traits and delivered to the PNNL and JGI within the same workday.

09 BIOMASS FUELS↗

Semi-automated Design of Artificial Intelligence Earth Systems Models

Prediction and observation of water cycles at various scales involve not only patterns isolated in space and time, but also modeling of complex spatio-temporal relationships across multiple domains. For instance, evapotranspiration (ET) and leaf area indexes (LAI) are two parameters that are needed to accurately model and understand land-atmosphere processes. Accurate assessments of ET and LAI are critical for understanding hydrological processes, deforestation, crop yield, and irrigation impacts. However, ET estimates for global simulations are available at very coarse spatial resolution. They are usually derived from satellite data based on broad plant functional types (PFTs), which fail to capture fine-scale variations because of changes in vegetation type across the globe. Similarly LAI estimates have typically been derived from vegetation indices at global scales or estimated locally using physical models, both of which suffer from a range of uncertainties that impact model sensitivity. The new era of AI model development for Earth systems (ES) calls for data-driven methods that provide domain scientists with uncertainty-aware estimations of biophysical parameters such as ET and LAI in a generalizable, interpretable, and discoverable manner.

54 ENVIRONMENTAL SCIENCES↗

Multispectral and Thermal Imager Onboard Aerial Platforms Instrument Handbook

The MicaSense Altum imager is an off-the-shelf, synchronized multispectral and thermal camera, combined with a Global Positioning System (GPS) unit and downwelling light sensor (DLS). The Altum takes images of the land surface within its field of view (FOV) across five visible bands and one longwave infrared thermal band. The sensor records the radiance and converts it to digital numbers. Imagery is radiometrically corrected, taking into account the sensor calibration, lens distortions, vignette effects, sun angle, and atmospheric effects (scattering and absorption). The photogrammetry software Agisoft PhotoScan v 1.4 is used to align and stitch the images into a larger composite image using the technique of structure from motion image capture to construct a dense cloud and 3D model of the surface, which is used to produce a digital elevation model of the terrain surveyed and orthomosaic imagery. Land surface leaf area index, albedo, and surface skin temperature are provided for the user. The user can perform raster calculations on the imagery to produce various other vegetative indices, which are used to indicate plant health and land surface characteristics, including Normalized Difference Vegetation Index (NDVI), Green Normalized Difference Vegetation Index (GNDVI), Normalized Difference Water Index (NDWI), surface temperature, and Enhanced_vegetation_index (EVI). This code is provided with the readme file for each data set. The images have a resolution of 20-60 cm/pixel, which are subsampled to 100 cm/pixel.

47 OTHER INSTRUMENTATION↗

Using satellite data to assess Hurricane Helene’s impact on vegetation by land use in the CSRA

Many regions within Georgia, South Carolina, and North Carolina experienced record-breaking rainfall and catastrophic winds due to Hurricane Helene. Helene made landfall on Florida’s big bend on September 26 th , 2024, as a category 4 hurricane, and tracked northward through Georgia and the southern Appalachian Mountains before dissipating on September 29 th , 2024. One significant impact of the hurricane was severe damage to tree canopies across the southeastern United States. This study utilizes satellite-based remotely sensed data provided by the National Aeronautical and Space Administration (NASA) to examine the resilience of these tree canopies following the hurricane. Specifically, the Normalized Difference Vegetation Index (NDVI), Leaf Area Index (LAI), Land Cover Type, Soil Moisture Active Passive (SMAP), and the Global Precipitation Measurement (GPM) mission datasets were employed to study the tree canopy’s response to such events in the Central Savannah River Area (CSRA). A significant increase was observed in the LAI in November of 2024. This increase in LAI was likely influenced by warmer-than-average temperatures throughout October of 2024, along with a second record-breaking rainfall event in the CSRA on November 6th, 2024. The LAI increase was then broken down into land cover type to understand which areas contributed most. The complex nature and resilience of trees became evident, offering opportunities for further exploration and application in urban planning, emergency response, and environmental management.

54 ENVIRONMENTAL SCIENCES↗

Validation of the Community Land Model Version 5 over the Contiguous United States (CONUS) using in-situ and remote sensing datasets

The Community Land Model (CLM) is an effective tool to simulate the biophysical and biogeochemical processes and their interactions with the atmosphere. Although CLM Version 5 (CLM5) constitutes various updates in these processes, its performance in simulating energy, water and carbon cycles over the Contiguous United States (CONUS) at scales which land surface changes and hydrometeorological and hydroclimatological applications are more locally relevant is yet to be assessed. In this study, we conducted three simulations at 0.125? during 1979-2018 over the CONUS using different configurations of CLM, namely CLM5-biogeochemistry (CLM5BGC), CLM4.5BGC, and CLM5-satellite phenology (CLM5SP). We validated and compared their simulations against multiple remote-sensed and in-situ datasets. Overall, the parametric and structural updates (e.g., carbon cost for nitrogen uptake, variable soil thickness, dry surface layer) in CLM5 improve its ability in capturing terrestrial biogeochemical dynamics. The low evapotranspiration in CLM5BGC is associated with biases in simulating vegetation phenological characteristics rather than soil water limitations. The mismatch between CLM5BGC-simulated peak leaf area index and reference data can be attributed to CLM5BGC's inability in simulating phenology of trees and grasses. The differences between CLM-simulated irrigation and reference estimates can be attributed to differences between processes represented in models and in reality, and uncertainties in input and validation datasets. Evaluation against observations at small catchments suggest that hydrologic parameters needed to be calibrated to improve simulations of runoff, especially subsurface runoff. Additional efforts are needed to incorporate spatially-distributed plant phenology and physiology parameters and regional-specific agricultural management practices (e.g., planting, harvest).

54 ENVIRONMENTAL SCIENCES↗

Chlorophyll fluorescence is a potential indicator to measure photochemical efficiency in early to late soybean maturity groups under changing day lengths and temperatures

In this study, we employed chlorophyll a fluorescence technique, to indicate plant health and status in response to changing day lengths (photoperiods) and temperatures in soybean early and late maturity groups. Chlorophyll a fluorescence study indicates changes in light reactions in photosystem II. Experiments were performed for 3-day lengths (12.5, 13.5, and 14.5 h) and five temperatures (22/14°C, 26/18°C, 30/22°C, 34/26°C, and 40/32°C), respectively. The I-P phase declined for changing day lengths. Active reaction centers decreased at long day length for maturity group III. We observed that low temperatures impacted the acceptor side of photosystem II and partially impacted electron transport toward the photosystem I end electron acceptor. Results emphasized that higher temperatures (40/32°C) triggered damage at the oxygen-evolving complex and decreased electron transport and photosynthesis. We studied specific leaf areas and aboveground mass. Aboveground parameters were consistent with the fluorescence study. Chlorophyll a fluorescence can be used as a potential technique for high-throughput phenotyping methods. The traits selected in the study proved to be possible indicators to provide information on the health status of various maturity groups under changing temperatures and day lengths. These traits can also be deciding criteria for breeding programs to develop inbreed soybean lines for stress tolerance and sensitivity based on latitudinal variations.

59 BASIC BIOLOGICAL SCIENCES↗

Impact of ultraviolet-B radiation on early-season morpho-physiological traits of indica and japonica rice genotypes

Ultraviolet (UV)-B radiation is considered one of the major detrimental rays coming from the Sun. UV-B radiation has a harmful impact on plant growth and development. The effect of UV-B radiation was studied on 64 rice (Oryza sativa L.) genotypes during the vegetative season. An equal number of genotypes from the japonica (50%) and indica (50%) subspecies were phenotyped using the Soil-Plant-Atmosphere-Research (SPAR) units. The 10 kJ UV-B was imposed 12 days after planting (DAP) and continued for three weeks (21 d). Based on the combined ultraviolet-B radiation response index (CUVBRI) for each genotype, the 64 rice genotypes were classified into sensitive, moderately sensitive, moderately tolerant, and tolerant. Various shoot traits, such as plant height, tiller, and leaf numbers, were measured. We also studied critical root phenological traits like root volume, diameter, tips, and forks. Out of all the studied shoot traits, leaf area showed maximum reduction for both indica (54%) and japonica (48%). Among the root traits, root length decreased by negligible (1%) for indica as compared to japonica (5%), while root crossing and forks showed a maximum decline for japonica (37 and 42%), respectively. This study is timely, meaningful, and required because it will help breeders select a tolerant or sensitive rice line for better yield and production under abiotic stresses.

59 BASIC BIOLOGICAL SCIENCES↗