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Large Scale Flame Spread Environmental Characterization Testing

Under the Advanced Exploration Systems (AES) Spacecraft Fire Safety Demonstration Project (SFSDP), as a risk mitigation activity in support of the development of a large-scale fire demonstration experiment in microgravity, flame-spread tests were conducted in normal gravity on thin, cellulose-based fuels in a sealed chamber. The primary objective of the tests was to measure pressure rise in a chamber as sample material, burning direction (upward/downward), total heat release, heat release rate, and heat loss mechanisms were varied between tests. A Design of Experiments (DOE) method was imposed to produce an array of tests from a fixed set of constraints and a coupled response model was developed. Supplementary tests were run without experimental design to additionally vary select parameters such as initial chamber pressure. The starting chamber pressure for each test was set below atmospheric to prevent chamber overpressure. Bottom ignition, or upward propagating burns, produced rapid acceleratory turbulent flame spread. Pressure rise in the chamber increases as the amount of fuel burned increases mainly because of the larger amount of heat generation and, to a much smaller extent, due to the increase in gaseous number of moles. Top ignition, or downward propagating burns, produced a steady flame spread with a very small flat flame across the burning edge. Steady-state pressure is achieved during downward flame spread as the pressure rises and plateaus. This indicates that the heat generation by the flame matches the heat loss to surroundings during the longer, slower downward burns. One heat loss mechanism included mounting a heat exchanger directly above the burning sample in the path of the plume to act as a heat sink and more efficiently dissipate the heat due to the combustion event. This proved an effective means for chamber overpressure mitigation for those tests producing the most total heat release and thusly was determined to be a feasible mitigation strategy to incorporate into the microgravity experiment.

Clayman, Lauren K.↗

Medical Lessons Learned from the Exploration Atmospheres Study

Background: The National Aeronautics and Space Administration’s (NASA) Exploration Atmospheres study (EA) was done to evaluate alternative cabin atmospheres for future spacecraft designs and planetary surface exploration of the Moon, Mars and beyond. Deep space exploration involves creating habitats and environments safe for human occupancy and means to explore the outside environment (extravehicular activities, EVA). In order to validate alternative atmospheres and pre-spacewalk procedures, the EA study was conducted to evaluate factors such as hypoxia risks, denitrogenation protocols, food limitations, medications, and the impact of other factors on human performance. Overview: Space travel is constrained by mass, volume, power and the cost of vehicle development, which creates tradeoffs in various capabilities, including breathing gasses. Higher atmospheric pressure in a vehicle means more gas, and a stronger containment vessel/habitat; while lower pressure requires higher oxygen partial pressure, which may increase fire risk. NASA’s EA study evaluated a proposed alternative cabin environment (8.2 psia, 34% Oxygen), for future spacecraft habitat, and planetary EVAs. EA included both a 3-day and a 11-day trial. These trials included a depressurization and saturation to 8.2psi at 34% O2 with additional depresses to 4.3 psia at 85% O2 for simulated EVAs, (1 EVA during the 3-day and 5 during the 11-day trials). Discussion: Planning for and executing the medical monitoring and response plan for a trial of this scope was a huge undertaking with no prior practice to fall back on. Food obstacles, sleeping issues, medications, joint injury, equipment limitations, medical privacy, multiple cases of decompression sickness, and even a COVID outbreak among the support team proved challenging. Conclusion: Testing of this nature is an essential part NASA’s preparation for the upcoming Lunar Artemis missions. As spaceflight transitions beyond low earth orbit, to planetary, even more trials of this nature will be required to learn what are the optimal atmospheric and associated operational constraints to maintain the optimal health of the crew and achieve mission objectives.

R Sanders↗

Observing System Simulations for the AOS Mission

The Earth System Observatory (ESO) is NASA’s response to the recommendations of the 2017 Earth Sciences Decadal Survey conducted by the US National Academy of Sciences, Engineering and Medicine. The ESO is being conceived as a set of fully integrated missions addressing 4 main Earth science focus areas including aerosols, clouds, convection and precipitation (jointly re-ferred to as AOS, the Atmosphere Observing System). ESO ground breaking observations will provide critical measurements to address societally relevant problems in climate change, natural hazard mitiga-tion, fighting forest fires, and improving real-time agricultural processes. A critical element of the AOS observing strategy is to make extensive use of new passive and active sen-sors as well as of the so-called Program-of-Record (PoR), complemented by a fully integrated sub-orbital component. In order to achieve maximum benefit, all these observations need to be integrated into comprehensive observing and modeling/data assimilation systems. Such an approach requires compre-hensive model-data synthesis capabilities that needs to be conceived in conjunction with the space-based and suborbital components of AOS. In this presentation we will summarize the major science goals of AOS including cloud feedbacks, at-mospheric convection, emphasizing aerosol processes and aerosol radiative effects, and the synergistic aspects of clouds-precipitation-aerosol interactions. We will describe examples of the observing system simulation capabilities being developed for AOS, including global storm resolving nature runs, detailed instrument and retrieval simulators, as well as fast retrieval emulators for instrumenting climate models. This simulation environment, being developed under NASA’s open-source science initiative, will permit us to explore how AOS data will be used across space and time to better initialize forecasts and train modeling systems, and to infuse models and data assimilation systems with AOS data, well before launch.

Arlindo da Silva↗

Systems integration for the Kennedy Space Center (KSC) Robotics Applications Development Laboratory (RADL)

A laboratory for developing robotics technology for hazardous and repetitive Shuttle and payload processing activities is discussed. An overview of the computer hardware and software responsible for integrating the laboratory systems is given. The center's anthropomorphic robot is placed on a track allowing it to be moved to different stations. Various aspects of the laboratory equipment are described, including industrial robot arm control, smart systems integration, the supervisory computer, programmable process controller, real-time tracking controller, image processing hardware, and control display graphics. Topics of research include: automated loading and unloading of hypergolics for space vehicles and payloads; the use of mobile robotics for security, fire fighting, and hazardous spill operations; nondestructive testing for SRB joint and seal verification; Shuttle Orbiter radiator damage inspection; and Orbiter contour measurements. The possibility of expanding the laboratory in the future is examined.

Davis, V. Leon↗

4.5-kW Hall Effect Thruster Evaluated

As part of an Interagency Agreement with the Air Force Research Lab (AFRL), a space simulation test of a Russian SPT 140 Hall Effect Thruster was completed in September 1999 at Vacuum Facility 6 at the NASA Glenn Research Center at Lewis Field. The thruster was subjected to a three-part test sequence that included thrust and performance characterization, electromagnetic interference, and plume contamination. SPT 140 is a 4.5-kW thruster developed under a joint agreement between AFRL, Atlantic Research Corp, and Space Systems/Loral, and was manufactured by the Fakal Experimental Design Bureau of Russia. All objectives were satisfied, and the thruster performed exceptionally well during the 120-hr test program, which comprised 33 engine firings. The Glenn testing provided a critical contribution to the thruster development effort, and the large volume and high pumping speed of this vacuum facility was key to the test s success. The low background pressure (1 10 6 torr) provided a more accurate representation of space vacuum than is possible in most vacuum chambers. The facility had been upgraded recently with new cryogenic pumps and sputter shielding to support the active electric propulsion program at Glenn. The Glenn test team was responsible for all test support equipment, including the thrust stand, power supplies, data acquisition, electromagnetic interference measurement equipment, and the contamination measurement system.

Mason, Lee S.↗

Communications System Concept of Operations (ConOp) for Supporting Second Shift (SS) Operations

Wildland fires take place most often in remote areas without access to communications infrastructure. Communication during wildfire operations is crucial for safe and effective command and control of air assets, ground-based firefighters, and fire management. Without connectivity, Incident Command (IC) cannot exchange information, receive alerts or work with all parties involved in the wildfire suppression operation. Current policy directs IC to use voice radio communications, which require no prior infrastructure, to provide information needed for the command, control and safety of personnel and resources. Cellular communications are used only for logistical purposes unless no other method is available. To address some of the shortcomings of the current state of communications, the Advanced Capabilities for Emergency Response Operations (ACERO) Second Shift (SS) technical team is developing characteristics of an air-to-ground Mesh Radio System (MRS) and preparing for a future demonstration. The MRS will allow air and ground assets to automatically join a radio network without relying on existing infrastructure and exchange information critical to maintaining situation awareness and air traffic management by the onsite IC. The MRS will also give users more options for communications in addition to voice communications.

David Fuller↗

Status of the US Space Station ECLSS and internal TCS

The U.S. Space Station includes many elements and systems. Responsibility for these have been delegated to four NASA centers via a work packaging concept. Marshall Space Flight Center (MSFC) is responsible for work package one (WPOI) which includes the habitation, laboratory modules, the logistic carrier elements, as well as the environmental control and life support system (ECLSS), the internal thermal control system (ITCS), and the internal audio and video systems. Current status of the ECLSS and internal thermal are the subjects of this paper. The ECLSS is composed of six subsystem groups: the temperature humidity control, the atmosphere control and supply, the air revitalization, water reclamation and management, fecal waste management, and the fire detection and suppression subsystems. The internal TCS consists of all WP01 elements of passive and active thermal measures. The ECLSS and ITCS provide services for the permanently orbiting pressurized modules as well as the pressurized and unpressurized logistics carriers. The logistics carriers are used as ground-to-station ferries for both resupply and initial delivery of selected new equipment and later replacement items. Equipment, which cannot be subjected to the rigors of the space environment, will be carried in the pressurized logistic carrier; while an unpressurized carrier is used to resupply other commodities, such as external fluid consumables. The laboratory module houses the equipment for both payloads and subsystem maintenance and repair. The habitation module is dedicated to providing living and sleeping quarters including a galley, sleeping provisions, hygiene facilities, medical services as well as services for exercise and relaxation. The four nodes that join the two modules at their ends also house electronics as well as work stations and outside viewing cupolas. The airlocks, one providing an emergency hyperbaric chamber capability, are also attached. This assembly constitutes the U.S. provided portion of the initial station. Additional international modules and docking provisions for future attached pressurized payload as well as dual logistic module and growth capabilities are also provided. The primary heat rejection mechanism is achieved via a central set of radiators provided by another work package. The interface with the central TCS at the module heat exchangers defines the active control interface boundary of the ITCS with the central TCS. All internal cooling of the permanent orbital elements are provided via internal active fluid loops within the elements utilizing water as the transport media. Cabin and equipment cooling are provided by forced air flow and cold plate heat sinks. A special active cooling loop is provided in the laboratory module to accommodate experiments and other payloads. Coatings and insulation are used in conjunction with the active components to passively limit heat losses/gains. The most challenging ECLSS new technology issues relate to oxygen and water recovery. Closure of the oxygen loop requires the use of new regenerative CO 2 removal and concentration techniques, CO 2 reduction to convert the CO 2 to water with a waste by-product, and the electrolysis of water to produce breathing oxygen for water recovery. For water recovery three separate loops are currently planned. One loop provides potable water by utilizing a combination of recovered condensate and CO 2 reduction water. A second loop purifies hygiene water from waste from the showers, clothes washer, hygiene/hand wash stations and a dishwasher. The third loop provides hygiene water by the reclamation and purification of urine water. This paper outlines the basic services provided by the ECLSS and ITCS and describes the ECLSS functional distribution throughout the pressurized assembly.

R. Humphries↗

Transient Three-Dimensional Startup Side Load Analysis of a Regeneratively Cooled Nozzle

The objective of this effort is to develop a computational methodology to capture the startup side load physics and to anchor the computed aerodynamic side loads with the available data from a regeneratively cooled, high-aspect-ratio nozzle, hot-fired at sea level. The computational methodology is based on an unstructured-grid, pressure-based, reacting flow computational fluid dynamics and heat transfer formulation, a transient 5 s inlet history based on an engine system simulation, and a wall temperature distribution to reflect the effect of regenerative cooling. To understand the effect of regenerative wall cooling, two transient computations were performed using the boundary conditions of adiabatic and cooled walls, respectively. The results show that three types of shock evolution are responsible for side loads: generation of combustion wave; transitions among free-shock separation, restricted-shock separation, and simultaneous free-shock and restricted shock separations; along with the pulsation of shocks across the lip, although the combustion wave is commonly eliminated with the sparklers during actual test. The test measured two side load events: a secondary and lower side load, followed by a primary and peak side load. Results from both wall boundary conditions captured the free-shock separation to restricted-shock separation transition with computed side loads matching the measured secondary side load. For the primary side load, the cooled wall transient produced restricted-shock pulsation across the nozzle lip with peak side load matching that of the test, while the adiabatic wall transient captured shock transitions and free-shock pulsation across the lip with computed peak side load 50% lower than that of the measurement. The computed dominant pulsation frequency of the cooled wall nozzle agrees with that of a separate test, while that of the adiabatic wall nozzle is more than 50% lower than that of the measurement. The computed teepee-like formation and the tangential motion of the shocks during lip pulsation also qualitatively agree with those of test observations. Moreover, a third transient computation was performed with a proportionately shortened 1 s sequence, and lower side loads were obtained with the higher ramp rate.

Wang, Ten-See↗

Arizona Water Resources: Utilizing Aerial Imagery and NASA Earth Observations to Assess Pinyon-Juniper Tree Mortality in Flagstaff, AZ

Pinyon-juniper woodlands (PJW) are a vital habitat and food source for several wildlife species and a source of both utility and cultural importance for Indigenous groups. In 2021, amidst a decades-long drought, an extensive juniper mortality event occurred at Wupatki National Monument (WNM) in Arizona. In response, the National Park Service (NPS) is evaluating which land management practices will be beneficial. In partnership with the NPS, the NASA DEVELOP team used remote sensing data to map PJW mortality and analyze the relation of tree mortality to stand density, climate, and topography in north-central Arizona from 2015 to 2021. To identify the extent of PJW, the team performed an unsupervised classification using National Agricultural Imagery Program (NAIP) data with validation sources including NPS-created land cover maps, Landscape Fire and Resource Management Planning Tools (LANDFIRE), NPS and United States Forest Service (USFS) vegetation maps, and Landsat 8 Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS) data. Terra Moderate Resolution Imaging Spectroradiometer (MODIS), Global Precipitation Measurement (GPM) Integrated Multi-satellite Retrievals for GPM (IMERG), Soil Moisture Active Passive (SMAP), Shuttle Radar Topography Mission (SRTM), and Landsat 8-derived Normalized Difference Vegetation Index (NDVI) and Normalized Difference Moisture Index (NDMI) were used to analyze factors contributing to pinyon-juniper mortality. Although no relationships were found in the broader study region, PJW mortality was weakly correlated to elevation, soil moisture, and land surface temperature within WNM. Results from this study can inform NPS vegetation management that best protects natural and cultural resources.

Margaret Jaenicke↗

Shock response spectra variational analysis for pyrotechnic qualification testing of flight hardware

Shock response spectra data from flight certification tests were analyzed to determine envelope variation with respect to mean values in each axis. An overall variation of + or - 8.61 dB or 169 percent exists for the data. This large variation may be attributed to one or more of the following: (1) Instrumentation problems may exist. (2) Variations in the source charge (blasting caps) such as shape or explosive load may exist. (3) Two blasting caps were used to excite the pyrotechnic plate tester. Delay time between charge firings may have varied. The cause or causes of the variations need to be identified and researched to prevent future pyroshock problems.

Smith, J. L.↗

Flood Impacts on Net Ecosystem Exchange in the Midwestern and Southern United States in 2019

Climate extremes such as droughts, floods, heatwaves, frosts, and windstorms add considerable variability to the global year-to-year increase in atmospheric CO(2) through their influence on terrestrial ecosystems. While the impact of droughts on terrestrial ecosystems has received considerable attention, the response to flooding is not well understood. To improve upon this knowledge, the impact of the 2019 anomalously wet conditions over the Midwest and Southern US on CO(2) vegetation fluxes is examined in the context of 2017–2018 when such precipitation anomalies were not observed. CO(2) is simulated with NASA's Global Earth Observing System (GEOS) combined with the Low-order Flux Inversion, where fluxes of CO(2) are estimated using a suite of remote sensing measurements including greenness, night lights, and fire radiative power as well as with a bias correction based on insitu observations. Net ecosystem exchange CO(2) tracers are separated into the three regions covering the Midwest, South, and Eastern Texas and adjusted to match CO(2) observations from towers located in Iowa, Mississippi, and Texas. Results indicate that for the Midwestern region consisting primarily of corn and soybeans crops, flooding contributes to a 15%–25% reduction of annual net carbon uptake in 2019 in comparison to 2017 and 2018. These results are supported by independent reports of changes in agricultural activity. For the Southern region, comprised mainly of non-crop vegetation, annual net carbon uptake is enhanced in 2019 by about 10%–20% in comparison to 2017 and 2018. These outcomes show the heterogeneity in effects that excess wetness can bring to diverse ecosystems.

Nikolay Balashov↗

Development of the European Service Module Propulsion Subsystem for the Multi-Purpose Crew Vehicle

2013, NASA and the European Space Agency (ESA) entered into an international partnership to develop the European Service Module (ESM) for use on NASA's Multi-Purpose Crew Vehicle (MPCV), also known as Orion. The MPCV will be used as the principal spacecraft for future human space exploration missions beyond low earth orbit. The ESM Propulsion Subsystem (PSS) is a pressure-fed, bi-propellant propulsion system, being developed by Airbus Defense and Space under contract to ESA. For this effort, NASA is responsible for the traditional role of insight/oversight to ensure that the PSS delivered by Airbus meets all MPCV Program requirements. In addition, the NASA Propulsion team also has some unique responsibilities that are a result of the Implementing Agreement (IA) between NASA and ESA for development of the ESM. These responsibilities include: (1) providing the main engine and Thrust Vector Control (TVC) assembly for the PSS. This is being accomplished through the delta qualification and re-use the Space Shuttle Orbital Maneuvering System (OMS) engine and TVC assembly; (2) procurement and delivery of the Auxiliary engines (R-4Ds) for the PSS. These engines are being procured by NASA from Aerojet-Rocketdyne via Lockheed Martin, the prime contractor for the MPCV, per an Airbus-provided specification; and (3) conducting the integrated systems hot-fire test which will qualify the end-to-end PSS for flight on MPCV. This test is being conducted at the NASA White Sands Test Facility (WSTF) using an Airbus-provided test article known as the Propulsion Qualification Model (PQM).

Multi-Purpose Crew Vehicle↗

Novel Carbon Dioxide Microsensor Based on Tin Oxide Nanomaterial Doped With Copper Oxide

Carbon dioxide (CO2) is one of the major indicators of fire and therefore its measurement is very important for low-false-alarm fire detection and emissions monitoring. However, only a limited number of CO2 sensing materials exist due to the high chemical stability of CO2. In this work, a novel CO2 microsensor based on nanocrystalline tin oxide (SnO2) doped with copper oxide (CuO) has been successfully demonstrated. The CuO-SnO2 based CO2 microsensors are fabricated by means of microelectromechanical systems (MEMS) technology and sol-gel nanomaterial-synthesis processes. At a doping level of CuO: SnO2 = 1:8 (molar ratio), the resistance of the sensor has a linear response to CO2 concentrations for the range of 1 to 4 percent CO2 in air at 450 C. This approach has demonstrated the use of SnO2, typically used for the detection of reducing gases, in the detection of an oxidizing gas.

Xu, Jennifer C.↗

NASA's Land, Atmosphere Near Real-Time Capability for EOS (LANCE): Delivering Data and Imagery to Meet the Needs of Near Real-Time Applications

NASA's Land, Atmosphere Near real-time Capability for EOS (LANCE) is a virtual system that provides near real-time EOS data and imagery from the AIRS, AMSR2, LIS (ISS), MISR, MLS, MODIS, MOPITT, OMI, OMPS, and VIIRS instruments, to meet the needs of scientists and application users interested in monitoring a wide variety of natural and man-made phenomena. NRT imagery from LANCE are available through NASA's Global Imagery Browse Services (GIBS), Worldview, FIRMS and most recently through Worldview Snapshots – a low band width application that has replaced the Rapid Response Subsets. Over the past year: data and imagery from the Lightning Imaging Sensor (LIS) on board the International Space Station (ISS), OMPS and VIIRS-Land have been added to LANCE. In the coming year LANCE will integrate the MODIS NRT Global Flood product, VIIRS Black Marble nighttime lights and Cloud Mask and Aerosol Dark Target from VIIRS Atmosphere. Here we provide a brief overview of LANCE, focusing on what's new and describing how these new data sets have been used to monitor lightning flashes, hurricanes and fires. For more information on LANCE visit: https://earthdata.nasa.gov/lance.

Davies, Diane↗

A Role of the Reaction Kernel in Propagation and Stabilization of Edge Diffusion Flames of C1-C3 Hydrocarbons

Diffusion flame stabilization is of essential importance in both Earth-bound combustion systems and spacecraft fire safety. Local extinction, re-ignition, and propagation processes may occur as a result of interactions between the flame zone and vortices or fire-extinguishing agents. By using a computational fluid dynamics code with a detailed chemistry model for methane combustion, the authors have revealed the chemical kinetic structure of the stabilizing region of both jet and flat-plate diffusion flames, predicted the flame stability limit, and proposed diffusion flame attachment and detachment mechanisms in normal and microgravity. Because of the unique geometry of the edge of diffusion flames, radical back-diffusion against the oxygen-rich entrainment dramatically enhanced chain reactions, thus forming a peak reactivity spot, i.e., reaction kernel, responsible for flame holding. The new results have been obtained for the edge diffusion flame propagation and attached flame structure using various C1-C3 hydrocarbons.

Takahashi, Fumiaki↗

Fiber-Optic Determination of N2, O2, and Fuel Vapor in the Ullage of Liquid-Fuel Tanks

A fiber-optic sensor system has been developed that can remotely measure the concentration of molecular oxygen (O2), nitrogen (N2), hydrocarbon vapor, and other gases (CO2, CO, H2O, chlorofluorocarbons, etc.) in the ullage of a liquid-fuel tank. The system provides an accurate and quantitative identification of the above gases with an accuracy of better than 1 percent by volume (for O2 or N2) in real-time (5 seconds). In an effort to prevent aircraft fuel tank fires or explosions similar to the tragic TWA Flight 800 explosion in 1996, OBIGGS are currently being developed for large commercial aircraft to prevent dangerous conditions from forming inside fuel tanks by providing an inerting gas blanket that is low in oxygen, thus preventing the ignition of the fuel/air mixture in the ullage. OBIGGS have been used in military aircraft for many years and are now standard equipment on some newer large commercial aircraft (such as the Boeing 787). Currently, OBIGGS are being developed for retrofitting to existing commercial aircraft fleets in response to pending mandates from the FAA. Most OBIGGS use an air separation module (ASM) that separates O2 from N2 to make nitrogen-enriched air from compressed air flow diverted from the engine (bleed air). Current OBIGGS systems do not have a closed-loop feedback control, in part, due to the lack of suitable process sensors that can reliably measure N2 or O2 and at the same time, do not constitute an inherent source of ignition. Thus, current OBIGGS operate with a high factor-of-safety dictated by process protocol to ensure adequate fuel-tank inerting. This approach is inherently inefficient as it consumes more engine bleed air than is necessary compared to a closed-loop controlled approach. The reduction of bleed air usage is important as it reduces fuel consumption, which translates to both increased flight range and lower operational costs. Numerous approaches to developing OBIGGS feedback-control sensors have been under development by many research groups and companies. However, the direct measurement of nitrogen (N2) is a challenge to most OBIGGS ullage sensors (such as tunable diode laser absorption) as they cannot measure N2 directly but depend on the measurement of oxygen (O2). The problem with a singular measure of O2, is that as the concentration (number density) of O2 decreases due to the inerting process or due to lower pressures from high altitudes, the precision and accuracy of the O2 measurement decreases. However, measuring O2 density in combination with N2 density (which is more abundant in air and in a N2-inerted fuel tank) can provide a much more accurate and reliable determination of the OBIGGS efficacy.

Nguyen, Quang-Viet↗

Structural dynamics research in a full-scale transport aircraft crash test

A remotely piloted air-to-ground crash test of a full-scale transport aircraft was conducted for the first time for two purposes: (1) to demonstrate performance of an antimisting fuel additive in suppressing fire in a crash environment, and (2) to obtain structural dynamics data under crash conditions for comparison with analytical predictions. The test, called the Controlled Impact Demonstration (CID), was sponsored by FAA and NASA with cooperation of industry, the Department of Defense, and the British and French governments. The test aircraft was a Boeing 720 jet transport. The aircraft impacted a dry lakebed at Edwards Air Force Base, CA. The purpose of this paper is to discuss the structural aspects of the CID. The fuselage section tests and the CID itself are described. Structural response data from these tests are presented and discussed. Nonlinear analytical modeling efforts are described, and comparisons between analytical results and experimental results are presented.

Mccomb, H. G., Jr.↗

Effects of litter addition on ectomycorrhizal associates of a lodgepole pine (Pinus contorta) stand in Yellowstone National Park

Increasing soil nutrients through litter manipulation, pollution, or fertilization can adversely affect ectomycorrhizal (EM) communities by inhibiting fungal growth. In this study, we used molecular genetic methods to determine the effects of litter addition on the EM community of a Pinus contorta stand in Yellowstone National Park that regenerated after a stand-replacing fire. Two controls were used; in unmodified control plots nothing was added to the soil, and in perlite plots perlite, a chemically neutral substance, was added to maintain soil moisture and temperature at levels similar to those under litter. We found that (i) species richness did not change significantly following perlite addition (2.6 +/- 0.3 species/core in control plots, compared with 2.3 +/- 0.3 species/core in perlite plots) but decreased significantly (P < 0.05) following litter addition (1.8 +/- 0.3 species/core); (ii) EM infection was not affected by the addition of perlite but increased significantly (P < 0.001) in response to litter addition, and the increase occurred only in the upper soil layer, directly adjacent to the added litter; and (iii) Suillus granulatus, Wilcoxina mikolae, and agaricoid DD were the dominant organisms in controls, but the levels of W. mikolae and agaricoid DD decreased significantly in response to both perlite and litter addition. The relative levels of S. granulatus and a fourth fungus, Cortinariaceae species 2, increased significantly (P < 0.01 and P < 0.05, respectively) following litter addition. Thus, litter addition resulted in some negative effects that may be attributable to moisture-temperature relationships rather than to the increased nutrients associated with litter. Some species respond positively to litter addition, indicating that there are differences in their physiologies. Hence, changes in the EM community induced by litter accumulation also may affect ecosystem function.

Fungi/classification/growth & development↗