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At least 487 records · Page 27

STS-112 Mission Highlights Resource Tape Part 2 of 3

An overview of the STS-112 Mission is presented. The fourth flight day begins with a view inside of the Destiny Laboratory of the International Space Station where Expedition Five Commander Valery Korzun is shown. The robotics workstation where Mission Specialist Sandra Mangus and Flight Engineer Peggy Whitson operate Canadarm 2 to lift the S(1) Truss segment out of the payload bay of the Space Shuttle Atlantis and maneuver it for installation onto the S(1) Truss is presented. Mission Specialist Piers Sellers is shown preparing for his six and one half hour spacewalk by performing breathing exercises. Animation of the installation of the S(1) Truss, and also the unbirthing of the S(1) truss is also presented. Mission Specialists David Wolf and Piers Sellers are shown getting suited for their spacewalks. During the spacewalk, David Wolf is removing the antenna assembly from its launched position and Piers Sellers is releasing launch restraints from the Radiator Beam Assembly. A beautiful view of the coast of Texas is captured during this spacewalk. Flight day five shows pilot Pam Melroy and Mission Specialist Dave Wolf working inside of the International Space Station's Quest Airlock. Flight day six is shown with Dave Wolf and Piers Sellers exiting their spacesuits inside of the Quest Airlock Module after a successful spacewalk. This presentation ends with views of the International Space Station's installed S(1) Truss, rotation of the radiator assembly and the radiator's coolant tubing.

Source record↗

NBIC Biofeeds: A Digital Tool for Open Source Biosurveillance across Federal Agencies

The National Biosurveillance Integration Center (NBIC) is developing a scalable, flexible open source data collection, analysis, and dissemination tool to support biosurveillance operations by the U.S. Department of Homeland Security (DHS) and its federal interagency partners. The NBIC integrates, analyzes, and distributes key information about health and disease events to help ensure the nation’s responses are well-informed, save lives, and minimize economic impact. NBIC serves as a bridge between Federal, State, Local, Territorial, and Tribal entities to conduct biosurveillance across human, animal, plant, and environmental domains. The integration of information enables early warning and shared situational awareness of biological events to inform critical decisions directing response and recovery efforts. To meet its mission objectives, NBIC utilizes a variety of datasets, including open source information, to provide comprehensive coverage of biological events occurring across the globe. NBIC Biofeeds is a digital tool designed to improve the efficiency of reviewing and analyzing large volumes of open source reporting by biosurveillance analysts on a daily basis; moreover, the system provides a mechanism to disseminate tailored feeds allowing NBIC to better meet the specific information needs of individual, interagency partners. The tool is currently under development by the Department of Energy (DOE), Pacific Northwest National Laboratory (PNNL)and it is in a testing and evaluation phase supported by NBIC biosurveillance subject matter experts. Integration with the Defense Threat Reduction Agency (DTRA), Biosurveillance Ecosystem(BSVE) is also underway. NBIC Biofeeds Version 1 is expected to be fully operational in Fiscal Year 2017. The PNNL is applying agile methodology to streamline the build of NBIC Biofeeds to specifications required for operational use by NBIC and its federal interagency partners. Biosurveillance, analytics, and system engineering subject matter experts provide guidance on the implementation of features in the tool to ensure functionality aligns with operational workflows and production support. PNNL is leveraging software from a previous government effort to repurpose the technology to meet NBIC needs. NBIC Biofeeds incorporates the open source, document-orientated MongoDB database to capture user- and system-generated metadata on hundreds of thousands of records, in part, to establish baselines to aid prospective and retrospective analysis on emerging biological events. NBIC Biofeeds integrates a biosurveillance taxonomy (uniquely developed by NBIC),which includes input from interagency partners to recognize critical characteristics of a biological event. In NBIC Biofeeds Version1, metadata capture of reported events is done manually by NBIC analysts; however, moving forward in Version 2, the tool will be further automated to flag significant reporting on biological events with a human remaining in the loop to confirm the validity of the system-generated tags. To serve as a one-stop tool for open source biosurveillance, NBIC Biofeeds automatically harvests information from thousands of websites, utilizing third party aggregators, paid subscriptions to data feeds, and scraping of high priority sources. Users can develop desired queries for automatic updating, leverage a unique review and curation mechanism, and further analyze data from topical, geographic, and temporal visualization features in the tool. To meet NBIC’s information sharing needs, the tool allows for design of tailored RSS feeds and electronic message-based delivery of analysis on biological events, intended for recipients in the government with unique missions around human, animal, plant, and environmental health. Through current testing and evaluation – underway by biosurveillance subject matter experts – NBIC Biofeeds is demonstrating value in supporting open source biosurveillance by the Center for more rapid recognition and sharing of key event characteristics. Centralizing access and analysis of this data set into a single system is increasing the efficiency of daily, global biosurveillance, while enhancing the value of information identified through use of the querying, curation, and production support features in the tool.

59 BASIC BIOLOGICAL SCIENCES↗

In planta single-molecule imaging and holographic force spectroscopy to study real-time, multimodal turnover dynamics of polysaccharides and associated carbohydrate metabolites

Cellular production of polysaccharides from simple sugar molecules serves critical roles in a variety of metabolic processes essential to the survival of every living organism. In the context of photosynthetically fixed carbon, cell wall polysaccharides synthesis is particularly of interest for increased biomass accumulation for human food, animal feed, and bioenergy related applications. Despite a long history of research in plant cell biology, our understanding of in planta cell wall biosynthesis and its regulation is far from complete due to lack of a comprehensive microscopy toolkit that encompass the multiple spatial and temporal scales in which cell wall polysaccharides fibrils are synthesized and assembled into intricate cell wall networks. In this project, a multidisciplinary team of scientists from Rutgers University, Vanderbilt University, and Oak Ridge National Laboratory carried out innovative multimodal, multiscale microscopy studies of cell wall synthesis using single-molecule force-spectroscopy, super-resolution fluorescence microscopy, and in vivo live cell imaging. Our novel multimodal and holistic imaging approach revealed plant cell wall polysaccharides synthesis processes in unprecedented detail across multiple spatiotemporal scales, from a single-molecule to a single-cell. Besides, the team has made technical and scientific innovations across multiple research fields—microscopy, bioengineering, single-molecule biophysics, and plant biology, etc.—to accomplish the goals. The results from this project will greatly advance the mechanistic and holistic understanding of in planta cell wall synthesis, which will accelerate the development of better transgenic crops for bioenergy applications. Moreover, the new toolbox, combining powerful advanced microscopy assays with cell/protein engineering, will have broader impacts on molecular and cellular biology fields by paving the way for studying cellular processes occurring across multiple physical scales with multimodal microscopy methodologies.

59 BASIC BIOLOGICAL SCIENCES↗

Voyager Outreach Compilation

This NASA JPL (Jet Propulsion Laboratory) video presents a collection of the best videos that have been published of the Voyager mission. Computer animation/simulations comprise the largest portion of the video and include outer planetary magnetic fields, outer planetary lunar surfaces, and the Voyager spacecraft trajectory. Voyager visited the four outer planets: Jupiter, Saturn, Uranus, and Neptune. The video contains some live shots of Jupiter (actual), the Earth's moon (from orbit), Saturn (actual), Neptune (actual) and Uranus (actual), but is mainly comprised of computer animations of these planets and their moons. Some of the individual short videos that are compiled are entitled: The Solar System; Voyage to the Outer Planets; A Tour of the Solar System; and the Neptune Encounter. Computerized simulations of Viewing Neptune from Triton, Diving over Neptune to Meet Triton, and Catching Triton in its Retrograde Orbit are included. Several animations of Neptune's atmosphere, rotation and weather features as well as significant discussion of the planet's natural satellites are also presented.

Source record↗

AniMACCS

AniMACCS is an animation software used in conjunction with MELCOR Accident Consequence Code System (MACCS), which is responsible for estimating the offsite consequences of potential severe accidents at nuclear power plants (NPPs) and of atmospheric releases of radioactive aerosols and gases from other nuclear facilities. MACCS is used to perform probabilistic health and economic consequence assessment of hypothetical releases of radioactive material. AniMACCS utilizes the MACCS output files to generate visual and geographical depictions of the accident consequences. SAND2020-12229 M Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc., for the U.S. Department of Energy’s National Nuclear Security Administration under contract DE-NA0003525.

Gallagher, LInda↗

Future human bone research in space

Skylab crewmembers demonstrated negative calcium (Ca) balance reaching about -300 mg/day by flight day 84. Limited bone density (BMD) measurements documented that bone was not lost equally from all parts of the skeleton. Subsequent BMD studies during long duration Russian flights documented the regional extent of bone loss. These studies demonstrated mean losses in the spine, femur neck, trochanter, and pelvis of about 1%-1.6% with large differences between individuals as well as between bone sites in a given individual. Limited available data indicate postflight bone recovery occurred in some individuals, but may require several years for complete restoration. Long duration bedrest studies showed a similar pattern of bone loss and calcium balance (-180 mg/day) as spaceflight. During long duration bedrest, resorption markers were elevated, formation markers were unchanged, 1,25 vitamin D (VitD) and calcium absorption were decreased, and serum ionized Ca was increased. Although this information is a good beginning, additional spaceflight research is needed to assess architectural and subregional bone changes, elucidate mechanisms, and develop efficient as well as effective countermeasures. Space research poses a number of unique problems not encountered in ground-based laboratory research. Therefore, researchers contemplating human spaceflight research need to consider a number of unique problems related to spaceflight in their experimental design.

NASA Center JSC↗

Development of a Metabolic Cage for Simulation of Weightlessness in a Laboratory Environment

Astronauts experience many physiological changes during spaceflight and exposure to microgravity. There is a headwardshift of fluids, muscles atrophy, and changes occur in the body's bone structure and hormone levels. This paper describes a unique metabolic cage that was developed to evaluate ground simulation of these effects. Hindlimb or tail suspension in rats has been studied as a model to create the effects of microgravity in order to study how to counteract them. This suspension model, developed by Holton, accurately simulates the effects of weightlessness during spaceflight by unloading the hindlimbs and producing a head-ward shift of fluids. However, obtaining quantitative data on the nutritional state, the gastrointestinal and renal function of these animals has not been possible, until now. Using Holton's tail suspension model, the new metabolic suspension cage effectively separates urine and feces samples for analysis allowing investigators to examine the effects of microgravity in many complex body systems. A description of the cage system, its design, and results of its use is provided along with pictures and details for replication of the cages.

Mulenburg, Gerald M.↗

Synchrotron-based X-ray fluorescence microscopy mapping the ionome of a toxic freshwater cyanobacterium

Harmful algal blooms (HABs) pose a major environmental concern across the globe. In abundance, cyanobacteria, or so-called green-blue algae can produce extremely dangerous cyanotoxins that harm humans and animals. This study focused on the mapping and distribution of intracellular macro-and micronutrients of the widespread freshwater cyanobacteria Microcystis aeruginosa (M. aeruginosa). Towards a better understanding of trace metal uptake and homeostasis throughout the cell cycle, we quantitatively mapped the spatial distribution of the elements P, K, Fe, Ca, Zn, Mn, and Cu across the ultrastructure of frozen-hydrated single cells using state-of-theart X-ray nanofluorescence imaging at the Advanced Photon Source (APS) at Argonne National Laboratory. Further, bulk cellular nutrient and trace metal content correlated well with the total intracellular elemental content in individual cells obtained by quantitative synchrotron X-ray fluorescence measurements. Multi-dimensional mappings showed P and K atoms colocalized as discrete semicircular hotspots that were analyzed with respect to their stoichiometry. Elevated Cu and Ca concentrations were detected along division plane of cells. P and K were found to have similar spatial elemental distribution with about 65% and 69% of the total cellular P and K, respectively, located at the hotspots. The P and K colocalization were refined further using nanotomography, showing a K envelope surrounding the P core. Inorganic P and organic P compounds were specified using solution-state 31 P nuclear magnetic resonance (NMR) spectroscopy from M. aeruginosa. Of the total extracted P determined by 31 P NMR spectroscopy, 47% were found to be nucleotides while only 11% were polyphosphates. Multimodal X-ray imaging provides a better understanding of intracellular biochemical processes in cyanobacteria, helping us monitor and combat an emerging environmental threat.

31P NMR spectroscopy↗

Polyamines as Possible Modulators of Gravity-induced Calcium Transport in Plants

Data from various laboratories indicate a probable relationship between calcium movement and some aspects of graviperception and tropistic bending responses. The movement of calcium in response to gravistimulation appears to be rapid, polar and opposite in direction to polar auxin transport. What might be the cause of such rapid Ca(2+) movement? Data from studies on polyamine (PA) metabolism may furnish a clue. A transient increase in the activity of ornithine decarboxylase (ODC) and titers of various PAs occurs within 60 seconds after hormonal stimulation of animal cells, followed by Ca(2+) transport out of the cells. Through the use of specific inhibitors, it was shown that the enhanced PA synthesis from ODC was essential not only for Ca(2+) transport, but also for Ca(2+) transport-dependent endocytosis and the movement of hexoses and amino acids across the plasmalemma. In plants, rapid changes in arginine decarboxylase (ADC) activity occur in response to various plant stresses. Physical stresses associated with gravisensor displacement and reorientation of a plant in the gravitational field could similarly activate ADC and that resultant increases in PA levels might initiate transient perturbations in Ca(2+) homeostasis.

Galston, A. W.↗

Effects of Gravity on Insect Circadian Rhythmicity

Circadian rhythms - endogenous daily rhythmic fluctuations in virtually all characteristics of life - are generated and coordinated by the circadian timing system (CTS). The CTS is synchronized to the external 24-hour day by time cues such as the light/dark cycle. In an environment without time cues, the length of an animal's day is determined by the period of its internal pacemaker (tau) and the animal is said to be free-running. All life on earth evolved under the solar day; the CTS exists as an adaptation that allows organisms to anticipate and to prepare for rhythmic environmental fluctuations. All life on earth also evolved under the force of earth's gravitational environment. While it is therefore not surprising that changes in the lighting environment affect the CTS, it is surprising that changes in the gravitational environment would do so. However, recent data from one of our laboratories using the brn-3.1 knockout mouse revealed that this model, which lacks the sensory receptor hair cells within the neurovestibular system, does not respond to exposure to a hyperdynamic environment in the same fashion as normal mice. The brn-3.1 mice did not show the expected suppression of circadian rhythmicity shown by control mice exposed to 2G. Exposure to altered ambient force environments affects the amplitude, mean and timing of circadian rhythms in species from unicellular organisms to man. In addition, there is a circadian influence on the homeostatic response to acute 2G acceleration and pulses of 2G can act as a time cue, synchronizing the CTS. This is of significance because maintenance of internal and external temporal coordination is critical for normal physiological and psychological function. Typically, during adaptation to an increased gravitational environment (+G), an initial acute reaction is followed by adaptation and, eventually, a new steady state (14-16), which can take weeks to months to establish. Until the development of space stations, exposure to microgravity was, of necessity, relatively short in duration. In early spaceflight experiments an organism's internal rhythms often expressed periods that were different from each other, even in the presence of a 24.0 hour light-dark cycle, suggesting that the organism was experiencing internal desynchronization (17, 18). In (micro)G, the body temperature rhythm was delayed with respect to other body rhythms and to the light-dark cycle in rhesus macaques (19) and man (20, 21). In the absence of a light-dark cycle, the circadian rhythm of spore formation persisted in Neurospora crassa, however, both the variability and average period of the rhythm increased (22). The beetle Trigonoscelis gigas, exhibited changes in period during and following 11-13 days in (micro)G (23, 24). Resynchronization of the urinary calcium rhythm following a 1800 phase shift of the LID cycle was retarded in rats exposed to (micro)G compared to 1G controls (25). With the development of the Russian Mir Space Station, long-term controlled microgravity exposure became possible. We recorded activity rhythms from black-bodied Tenebrionid beetles, Trigonoscelis gigas, in (micro)G (spaceflight). Each insect was housed individually within an activity monitor (26) and data (activity counts) were collected and stored in five-minute bins. Thirty-two individual activity monitors were housed within each of 2 experimental kits. The beetles within each kit were divided into two groups and the lighting was controlled separately for each group.

Hoban-Higgins, Tana M.↗

Magnetic microparticle-based multimer detection system for the detection of prion oligomers in sheep

Transmissible spongiform encephalopathies (TSEs) are zoonotic fatal neurodegenerative diseases in animals and humans. TSEs are commonly known as bovine spongiform encephalopathy in cattle, scrapie in sheep and goats, chronic wasting disease in cervids, and Creutzfeldt–Jakob disease in humans. The putative transmissible agents are infectious prion proteins (PrPSc), which are formed by the conversion of the normal prion protein on the glycoprotein cell surface in the presence of other PrPSc. Reports of the transmission of TSEs through blood raised considerable concern about the safety of blood and blood products. To address this issue, many laboratories attempted to develop a sensitive and accurate blood diagnostic test to detect PrPSc. Previously, we reported that, compared to normal controls, the multimer detection system (MDS) was more efficient in detecting PrPSc in infected hamster brain homogenate, mouse plasma spiked with purified PrPSc from scrapie mouse brain, and scrapie-infected hamster plasmas. MDS differentiates prion multimers from the cellular monomer through the multimeric expression of epitopes on prion multimers, in contrast to the monomeric form. In this study, MDS detected PrPSc in plasma samples from scrapie-infected sheep expressing clinical symptoms, demonstrating 100% sensitivity and specificity in these samples. Plasma samples from asymptomatic lambs at the preclinical stage (8-month-old naturally infected offspring of scrapie-infected parents expressing a highly susceptible genotype) tested positive with 50% sensitivity and 100% specificity. In the first of two coded analyses using clinical scrapie-infected sheep and normal healthy samples, MDS successfully identified all but one of the clinical samples with 92% sensitivity and 100% specificity. Similar results were obtained in the second coded analysis using preclinical samples. MDS again successfully identified all but one of the samples with 87% sensitivity and 100% specificity. The false-negative sample was subjected to a protease pretreatment. In conclusion, MDS could accurately detect scrapie in plasma samples at both preclinical and clinical stages. From these studies, we conclude that MDS could be a promising tool for the early diagnosis of TSEs from blood samples.

59 BASIC BIOLOGICAL SCIENCES↗

Radiation-induced instability and its relation to radiation carcinogenesis

PURPOSE: A model that identifies radiation-induced genetic instability as the earliest cellular event in the multi-step sequence leading to radiation-induced cancer was previously proposed. In this paper ongoing experiments are discussed which are designed to test this model and its predictions in mouse mammary epithelial cells. RESULTS: Several lines of evidence are presented that appear to support this model: first, the development of delayed mutations in p53 following irradiation in altered growth variants; secondly, the high frequencies for the induction of both instability and transformation following irradiation in mammary epithelial cells; and finally, the demonstration that susceptibility to the induction of cytogenetic instability is a heritable trait that correlates with susceptibility to transformation and radiation-induced mammary cancer. Mice resistant to transformation and mammary cancer development are also resistant to the development of instability after irradiation. In contrast, mice sensitive to transformation and cancer are also sensitive to the development of cytogenetic instability. CONCLUSIONS: Data from this laboratory and from the studies cited above suggest a specific, and perhaps unique, role for radiation-induced instability as a critical early event associated with initiation of the carcinogenic process.

Review↗

Minnowbrook V: 2006 Workshop on Unsteady Flows in Turbomachinery

This volume contains materials presented at the Minnowbrook V 2006 Workshop on Unsteady Flows in Turbomachinery, held at the Syracuse University Minnowbrook Conference Center, New York, on August 20-23, 2006. The workshop organizers were John E. LaGraff (Syracuse University), Martin L.G. Oldfield (Oxford University), and J. Paul Gostelow (University of Leicester). The workshop followed the theme, venue, and informal format of four earlier workshops: Minnowbrook I (1993), Minnowbrook II (1997), Minnowbrook III (2000), and Minnowbrook IV (2003). The workshop was focused on physical understanding of unsteady flows in turbomachinery, with the specific goal of contributing to engineering application of improving design codes for turbomachinery. The workshop participants included academic researchers from the United States and abroad and representatives from the gas-turbine industry and U.S. Government laboratories. The physical mechanisms discussed were related to unsteady wakes, active flow control, turbulence, bypass and natural transition, separation bubbles and turbulent spots, modeling of turbulence and transition, heat transfer and cooling, surface roughness, unsteady CFD, and DNS. The workshop summary and the plenary discussion transcripts clearly highlight the need for continued vigorous research in the technologically important area of unsteady flows in turbomachines. This volume contains abstracts and copies of select viewgraphs organized according to the workshop sessions. Full-color viewgraphs and animations are included in the CD-ROM version only (Doc.ID 20070024781).

LaGraff, John E.↗

Planetary Protection Bioburden Analysis Program

This program is a Microsoft Access program that performed statistical analysis of the colony counts from assays performed on the Mars Science Laboratory (MSL) spacecraft to determine the bioburden density, 3-sigma biodensity, and the total bioburdens required for the MSL prelaunch reports. It also contains numerous tools that report the data in various ways to simplify the reports required. The program performs all the calculations directly in the MS Access program. Prior to this development, the data was exported to large Excel files that had to be cut and pasted to provide the desired results. The program contains a main menu and a number of submenus. Analyses can be performed by using either all the assays, or only the accountable assays that will be used in the final analysis. There are three options on the first menu: either calculate using (1) the old MER (Mars Exploration Rover) statistics, (2) the MSL statistics for all the assays, or This software implements penetration limit equations for common micrometeoroid and orbital debris (MMOD) shield configurations, windows, and thermal protection systems. Allowable MMOD risk is formulated in terms of the probability of penetration (PNP) of the spacecraft pressure hull. For calculating the risk, spacecraft geometry models, mission profiles, debris environment models, and penetration limit equations for installed shielding configurations are required. Risk assessment software such as NASA's BUMPERII is used to calculate mission PNP; however, they are unsuitable for use in shield design and preliminary analysis studies. The software defines a single equation for the design and performance evaluation of common MMOD shielding configurations, windows, and thermal protection systems, along with a description of their validity range and guidelines for their application. Recommendations are based on preliminary reviews of fundamental assumptions, and accuracy in predicting experimental impact test results. The software is programmed in Visual Basic for Applications for installation as a simple add-in for Microsoft Excel. The user is directed to a graphical user interface (GUI) that requires user inputs and provides solutions directly in Microsoft Excel workbooks. This work was done by Shannon Ryan of the USRA Lunar and Planetary Institute for Johnson Space Center. Further information is contained in a TSP (see page 1). MSC- 24582-1 Micrometeoroid and Orbital Debris (MMOD) Shield Ballistic Limit Analysis Program Lyndon B. Johnson Space Center, Houston, Texas Commercially, because it is so generic, Enigma can be used for almost any project that requires engineering visualization, model building, or animation. Models in Enigma can be exported to many other formats for use in other applications as well. Educationally, Enigma is being used to allow university students to visualize robotic algorithms in a simulation mode before using them with actual hardware. This work was done by David Shores and Sharon P. Goza of Johnson Space Center; Cheyenne McKeegan, Rick Easley, Janet Way, and Shonn Everett of MEI Technologies; Mark Manning of PTI; and Mark Guerra, Ray Kraesig, and William Leu of Tietronix Software, Inc. For further information, contact the JSC Innovation Partnerships Office at (281) 483-3809. MSC-24211-1 Spitzer Telemetry Processing System NASA's Jet Propulsion Laboratory, Pasadena, California The Spitzer Telemetry Processing System (SirtfTlmProc) was designed to address objectives of JPL's Multi-mission Image Processing Lab (MIPL) in processing spacecraft telemetry and distributing the resulting data to the science community. To minimize costs and maximize operability, the software design focused on automated error recovery, performance, and information management. The system processes telemetry from the Spitzer spacecraft and delivers Level 0 products to the Spitzer Science Center. SirtfTlmProc is a unique system with automated error notification and recovery, with a real-time continuous service that can go quiescent after periods of inactivity. The software can process 2 GB of telemetry and deliver Level 0 science products to the end user in four hours. It provides analysis tools so the operator can manage the system and troubleshoot problems. It automates telemetry processing in order to reduce staffing costs. This work was done by Alice Stanboli, Elmain M. Martinez, and James M. McAuley of Caltech for NASA's Jet Propulsion Laboratory. For more information, contact iaoffice @jpl.nasa.gov. This software is available for commercial licensing. Please contact Dan Broderick at Daniel.F. Broderick@jpl.nasa.gov. Refer to NPO-47803. NASA Tech Briefs, September 2013 29 This rapid response computer program predicts Orbiter Wing Leading Edge (WLE) damage caused by ice or foam impact during a Space Shuttle launch (Program "IMPACT2"). The program was developed after the Columbia accident in order to assess quickly WLE damage due to ice, foam, or metal impact (if any) during a Shuttle launch. IMPACT2 simulates an impact event in a few minutes for foam impactors, and in seconds for ice and metal impactors. The damage criterion is derived from results obtained from one sophisticated commercial program, which requires hours to carry out simulations of the same impact events. The program was designed to run much faster than the commercial program with prediction of projectile threshold velocities within 10 to 15% of commercial-program values. The mathematical model involves coupling of Orbiter wing normal modes of vibration to nonlinear or linear springmass models. IMPACT2 solves nonlinear or linear impact problems using classical normal modes of vibration of a target, and nonlinear/ linear time-domain equations for the projectile. Impact loads and stresses developed in the target are computed as functions of time. This model is novel because of its speed of execution. A typical model of foam, or other projectile characterized by material nonlinearities, impacting an RCC panel is executed in minutes instead of hours needed by the commercial programs. Target damage due to impact can be assessed quickly, provided that target vibration modes and allowable stress are known. This work was done by Robert Clark, Jr., Paul Cotter, and Constantine Michalopoulos of The Boeing Company for Johnson Space Center. For further information, contact the JSC Innovation Partnerships Office at (281) 483-3809. MSC-24988-1 Wing Leading Edge RCC Rapid Response Damage Prediction Tool (IMPACT2) Lyndon B. Johnson Space Center, Houston, Texas (3) the MSL statistics for only the accountable assays. Other options on the main menu include a data editing form and utility programs that produce various reports requested by the microbiologists and the project, and tools to generate the groupings for the final analyses. The analyses can be carried out in three ways: Each assay can be treated separately, the assays can be collectively treated for the whole zone as a group, or the assays can be collected in groups designated by the JPL Planetary Protection Manager. The latter approach was used to generate the final report because assays on the same equipment or similar equipment can be assumed to have been exposed to the same environment and cleaning. Thus, the statistics are improved by having a larger population, thereby reducing the standard deviation by the square root of N. For each method mentioned above, three reports are available. The first is a detailed report including all the data. This version was very useful in verifying the calculations. The second is a brief report that is similar to the full detailed report, but does not print out the data. The third is a grand total and summary report in which each assay requires only one line. For the first and second reports, most of the calculations are performed in the report section itself. For the third, all the calculations are performed directly in the query bound to the report. All the numerical results were verified by comparing them with Excel templates, then exporting the data from the Planetary Protection Analysis program to Excel.

Beaudet, Robert A.↗

2010 Ecological Survey of the Pacific Northwest National Laboratory Site

The U.S. Department of Energy (DOE) Pacific Northwest Site Office (PNSO) oversees and manages the DOE contract for the Pacific Northwest National Laboratory (PNNL), a DOE Office of Science multi-program laboratory located in Richland, Washington. PNSO is responsible for ensuring that all activities conducted on the PNNL Site comply with applicable laws, policies, and DOE orders. The DOE Pacific Northwest Site Office Cultural and Biological Resources Management Plan (DOE/PNSO 2008) addresses the requirement for annual surveys and monitoring for species of concern and to identify and map invasive species. In addition to the requirement for an annual survey, proposed project activities must be reviewed to assess any potential environmental consequences of conducting the project. The assessment process requires a thorough understanding of the resources present, the potential impacts of a proposed action to those resources, and the ultimate consequences of those actions. The PNNL Site is situated on the southeastern corner of the DOE Hanford Site, located at the north end of the city of Richland in south-central Washington. The site is bordered on the east by the Columbia River, on the west by Stevens Drive, and on the north by the Hanford Site 300 Area (Figure 1). The environmental setting of the PNNL Site is described in Larson and Downs (2009). There are currently two facilities on the PNNL Site: the William R. Wiley Environmental Molecular Sciences Laboratory (EMSL), and the recently completed Physical Sciences Facility (PSF). This report describes the results of the annual survey of the biological resources found on the undeveloped portions of the PNNL Site in 2010. A brief description of the methods PNNL ecologists used to conduct the surveys and the results of the surveys are presented. Actions taken to fully delineate noxious weed populations discovered in 2009 and efforts in 2010 to control those weeds also are described. Appendix A provides a list of plant and animal species identified on the PNNL Site.

54 ENVIRONMENTAL SCIENCES↗

Cutaneous and local radiation injuries

The threat of a large-scale radiological or nuclear (R/N) incident looms in the present-day climate, as noted most recently in an editorial in Scientific American (March 2021). These large-scale incidents are infrequent but affect large numbers of people. Smaller-scale R/N incidents occur more often, affecting smaller numbers of people. There is more awareness of acute radiation syndrome (ARS) in the medical community; however, ionising radiation-induced injuries to the skin are much less understood. Here, this article will provide an overview of radiation-induced injuries to the skin, deeper tissues, and organs. The history and nomenclature; types and causes of injuries; pathophysiology; evaluation and diagnosis; current medical management; and current research of the evaluation and management are presented. Cutaneous radiation injuries (CRI) or local radiation injuries (LRI) may lead to cutaneous radiation syndrome, a sub-syndrome of ARS. These injuries may occur from exposure to radioactive particles suspended in the environment (air, soil, water) after a nuclear detonation or an improvised nuclear detonation (IND), a nuclear power plant incident, or an encounter with a radioactive dispersal or exposure device. These incidents may also result in a radiation-combined injury; a chemical, thermal, or traumatic injury, with radiation exposure. Skin injuries from medical diagnostic and therapeutic imaging, medical misadministration of nuclear medicine or radiotherapy, occupational exposures (including research) to radioactive sources are more common but are not the focus of this manuscript. Diagnosis and evaluation of injuries are based on the scenario, clinical picture, and dosimetry, and may be assisted through advanced imaging techniques. Research-based multidisciplinary therapies, both in the laboratory and clinical trial environments, hold promise for future medical management. Great progress is being made in recognising the extent of injuries, understanding their pathophysiology, as well as diagnosis and management; however, research gaps still exist.

61 RADIATION PROTECTION AND DOSIMETRY↗

Comparative aspects of hematological responses in animal and human models in simulations of weightlessness and space flight

This paper reviews some human and animal responses to space flight as well as in control models in simulations of weightlessness. Astronauts after space flight have been found to have a decreased red blood cell mass and plasma volume. The reason for these changes is unknown but appears to be caused primarily by a decrease in the need of red blood cells in the weightless condition. Similar though more moderate changes have been found in human subjects subjected to prolonged bed rest or water immersion. What happens to the red cell mass of laboratory rats flown in microgravity is not known but rats have shown an increase in the rate of random red cell loss in flight suggesting a probable decrease. Rat models subjected to either head-down suspension or restraint alone have shown a decrease in red blood cell masses and a decrease in their plasma volume.

Lange, R. D.↗

STS-47 Post Flight Press Conference

The flight crew of the STS-47 Space Shuttle Orbiter Endeavour Cmdr. Robert L. Gibson, Pilot Curtis L. Brown, Payload Cmdr. Mark C. Lee, Mission Specialists, N. Jan Davis, Jay Apt, Mae C. Jemison, and Payload Specialist, Mamoru Mohri, present an overview of their mission. This the 50th Shuttle flight marks the first NASA mission devoted primarily to Japan. Endeavour carries into Earth orbit Spacelab-J (SL-J), a 23-foot long pressurized laboratory built by the European Space Agency specifically for conducting experiments in a shirt-sleeve environment. SL-J contains 43 experiments, 34 provided by Japan, 7 from the United States and 2 joint experiments. Jemison becomes the first African American woman to fly in space and Mohri first Japanese to fly in space. Video footage includes the following: prelaunch and launch activities; various experiments including protein crystal growth, electronic materials, fluids, glasses and ceramics, metals and alloys, and the effects of microgravity on plants and animals; earth views of Japan, Tokyo Harbor, and Hurricane Bonnie; and the re-entry and landing of the orbiter.

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