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NASA NTRS1983

Classifying Particles By Acoustic Levitation

Separation technique well suited to material processing. Apparatus with rectangular-cross-section chamber used to measure equilibrium positions of low-density spheres in gravitational field. Vertical acoustic forces generated by two opposing compression drivers exciting fundamental plane-wave mode at 1.2 kHz. Additional horizontal drivers centered samples along vertical axis. Applications in fusion-target separation, biological separation, and manufacturing processes in liquid or gas media.

Barmatz, Martin B.↗

NASA NTRS2004

The Living Universe: NASA and the Development of Astrobiology

In the opening weeks of 1998 a news article in the British journal Nature reported that NASA was about to enter biology in a big way. A "virtual" Astrobiology Institute was gearing up for business, and NASA administrator Dan Goldin told his external advisory council that he would like to see spending on the new institute eventually reach $100 million per year. "You just wait for the screaming from the physical scientists (when that happens)," Goldin was quoted as saying. Nevertheless, by the time of the second Astrobiology Science Conference in 2002, attended by seven hundred scientists from many disciplines, NASA spending on astrobiology had reached nearly half that amount and was growing at a steady pace. Under NASA leadership numerous institutions around the world applied the latest scientific techniques in the service of astrobiology's ambitious goal: the study of what NASA's 1996 Strategic Plan termed the "living universe." This goal embraced nothing less than an understanding of the origin, history, and distribution of life in the universe, including Earth. Astrobiology, conceived as a broad interdisciplinary research program, held the prospect of being the science for the twenty-first century which would unlock the secrets to some of the great questions of humanity. It is no surprise that these age-old questions should continue into the twenty-first century. But that the effort should be spearheaded by NASA was not at all obvious to those - inside and outside the agency - who thought NASA's mission was human spaceflight, rather than science, especially biological science. NASA had, in fact, been involved for four decades in "exobiology," a field that embraced many of the same questions but which had stagnated after the 1976 Viking missions to Mars. In this volume we tell the colorful story of the rise of the discipline of exobiology, how and why it morphed into astrobiology at the end of the twentieth century, and why NASA was the engine for both the discipline's founding and for its transformation.

Dick, Steven J.↗

NASA NTRS2004

High Sensitivity, Low Power Nano Sensors and Devices for Chemical Sensing

The chemical sensor market has been projected to grow to better than $40 billion dollars worldwide within the next 10 years. Some of the primary motivations to develop nanostructured chemical sensors are monitoring and control of environmental pollution; improved diagnostics for consumption; improvement in measurement precision and accuracy; and improved detection limits for Homeland security, battlefield environments, and process and quality control of industrial applications. In each of these applications, there is demand for sensitivity, selectivity and stability of environmental and biohazard detection and capture beyond what is currently commercially available. Nanotechnology offers the ability to work at the molecular level, atom by atom, to create large structures with fundamentally new molecular organization. It is essentially concerned with materials, devices, and systems whose structures and components exhibit novel and significantly improved physical, chemical and biological properties, phenomena, and process control due to their nanoscale size. One such nanotechnology-enabled chemical sensor has been developed at NASA Ames leveraging nanostructures, such as single walled carbon nanotubes (SWNTs) and metal oxide nanobelts or nanowires, as a sensing medium bridging a pair of interdigitated electrodes (IDE) realized through a silicon-based microfabrication and micromachining technique. The DE fingers are fabricated on a silicon substrate using standard photolithography and thin film metallization techniques. It is noteworthy that the fabrication techniques employed are not confined to the silicon substrate. Through spin casting and careful substrate selection (i.e. clothing, glass, polymer, etc.), additional degrees of freedom can be exploited to enhance sensitivity or to conform to unique applications. Both in-situ growth of nanostructured materials and casting of nanostructured dispersions were used to produce analogous chemical sensing devices.

Li, Jing↗

NASA NTRS2001

Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis

The lack of readily available experimental systems has limited knowledge pertaining to the development of Salmonella-induced gastroenteritis and diarrheal disease in humans. We used a novel low-shear stress cell culture system developed at the National Aeronautics and Space Administration in conjunction with cultivation of three-dimensional (3-D) aggregates of human intestinal tissue to study the infectivity of Salmonella enterica serovar Typhimurium for human intestinal epithelium. Immunohistochemical characterization and microscopic analysis of 3-D aggregates of the human intestinal epithelial cell line Int-407 revealed that the 3-D cells more accurately modeled human in vivo differentiated tissues than did conventional monolayer cultures of the same cells. Results from infectivity studies showed that Salmonella established infection of the 3-D cells in a much different manner than that observed for monolayers. Following the same time course of infection with Salmonella, 3-D Int-407 cells displayed minimal loss of structural integrity compared to that of Int-407 monolayers. Furthermore, Salmonella exhibited significantly lower abilities to adhere to, invade, and induce apoptosis of 3-D Int-407 cells than it did for infected Int-407 monolayers. Analysis of cytokine expression profiles of 3-D Int-407 cells and monolayers following infection with Salmonella revealed significant differences in expression of interleukin 1alpha (IL-1alpha), IL-1beta, IL-6, IL-1Ra, and tumor necrosis factor alpha mRNAs between the two cultures. In addition, uninfected 3-D Int-407 cells constitutively expressed higher levels of transforming growth factor beta1 mRNA and prostaglandin E2 than did uninfected Int-407 monolayers. By more accurately modeling many aspects of human in vivo tissues, the 3-D intestinal cell model generated in this study offers a novel approach for studying microbial infectivity from the perspective of the host-pathogen interaction.

NASA Discipline Environmental Health↗

NASA NTRS1989

Auxin physiology of the tomato mutant diageotropica

The tomato (Lycopersicon esculentum, Mill.) mutant diageotropica (dgt) exhibits biochemical, physiological, and morphological abnormalities that suggest the mutation may have affected a primary site of auxin perception or action. We have compared two aspects of the auxin physiology of dgt and wild-type (VFN8) seedlings: auxin transport and cellular growth parameters. The rates of basipetal indole-3-acetic acid (IAA) polar transport are identical in hypocotyl sections of the two genotypes, but dgt sections have a slightly greater capacity for IAA transport. 2,3,5-Triiodobenzoic acid and ethylene reduce transport in both mutant and wild-type sections. The kinetics of auxin uptake into VFN8 and dgt sections are nearly identical. These results make it unlikely that an altered IAA efflux carrier or IAA uptake symport are responsible for the pleiotropic effects resulting from the dgt mutation. The lack of auxin-induced cell elongation in dgt plants is not due to insufficient turgor, as the osmotic potential of dgt cell sap is less (more negative) than that of VFN8. An auxin-induced increase in wall extensibility, as measured by the Instron technique, only occurs in the VFN8 plants. These data suggest dgt hypocotyls suffer a defect in the sequence of events culminating in auxin-induced cell wall loosening.

NASA Discipline Plant Biology↗

NASA NTRS2005

Cardiovascular tissues contain independent circadian clocks

Acute cardiovascular events exhibit a circadian rhythm in the frequency of occurrence. The mechanisms underlying these phenomena are not yet fully understood, but they may be due to rhythmicity inherent in the cardiovascular system. We have begun to characterize rhythmicity of the clock gene mPer1 in the rat cardiovascular system. Luciferase activity driven by the mPer1 gene promoter is rhythmic in vitro in heart tissue explants and a wide variety of veins and arteries cultured from the transgenic Per1-luc rat. The tissues showed between 3 and 12 circadian cycles of gene expression in vitro before damping. Whereas peak per1-driven bioluminescence consistently occurred during the late night in the heart and all arteries sampled, the phases of the rhythms in veins varied significantly by anatomical location. Varying the time of the culture procedure relative to the donor animal's light:dark cycle revealed that, unlike some other rat tissues such as liver, the phases of in vitro rhythms of arteries, veins, and heart explants were affected by culture time. However, phase relationships among tissues were consistent across culture times; this suggests diversity in circadian regulation among components of the cardiovascular system.

Non-NASA Center↗

NASA NTRS2001

Microscale Particulate Classifiers (MiPAC) Being Developed

The NASA Glenn Research Center is developing microscale sensors to characterize atmospheric-borne particulates. The devices are fabricated using MEMS (microelectromechanical systems) technologies. These technologies are derived from those originally developed in support of the semiconductor processing industry. The resulting microsensors can characterize a wide range of particles and are, therefore, suitable to a broad range of applications. This project is supported under a collaborative program called the Glennan Microsystems Initiative. The initiative comprises members of NASA Glenn Research Center, various university affiliates from the State of Ohio, and a number of participating industrial partners. Funding is jointly provided by NASA, the State of Ohio, and industrial members. The work described here is a collaborative arrangement between researchers at Glenn, the University of Minnesota, The National Institute of Standards and Technology (NIST), and the Cleveland State University. Actual device fabrication is conducted at Glenn and at the laboratories of Case Western Reserve University. Case Western is also located in Cleveland, Ohio, and is a participating member of the initiative. The principal investigator for this project is Paul S. Greenberg of Glenn. Two basic types of devices are being developed, and target different ranges of particle sizes. The first class of devices, which is used to measure nanoparticles (i.e., particles in the range of 0.002 to 1 mm), is based on the technique of Electrical Mobility Classification. This technique also affords the valuable ability of measuring the electrical charge state of the particles. Such information is important in the understanding of agglomeration mechanisms and is useful in the development of methods for particle repulsion. The second type of device being developed, which utilizes optical scattering, is suitable for particles larger than 1 mm. This technique also provides information on particle shape and composition. Applications for these sensors include fundamental planetary climatology, monitoring and filtration in spacecraft, human habitation modules and related systems, characterization of particulate emissions from propulsion and power systems, and as early warning sensors for both space-based and ter-restrial fire detection. These devices are also suitable for characterizing biological compounds such as allergens, infectious agents, and biotoxic agents.

Greenberg, Paul S.↗

NASA NTRS1998

Biologically active protein fragments containing specific binding regions of serum albumin or related proteins

In accordance with the present invention, biologically active protein fragments can be constructed which contain only those specific portions of the serum albumin family of proteins such as regions known as subdomains IIA and IIIA which are primarily responsible for the binding properties of the serum albumins. The artificial serums that can be prepared from these biologically active protein fragments are advantageous in that they can be produced much more easily than serums containing the whole albumin, yet still retain all or most of the original binding potential of the full albumin proteins. In addition, since the protein fragment serums of the present invention can be made from non-natural sources using conventional recombinant DNA techniques, they are far safer than serums containing natural albumin because they do not carry the potentially harmful viruses and other contaminants that will be found in the natural substances.

Carter, Daniel C.↗

NASA NTRS2000

Selected contribution: a three-dimensional model for assessment of in vitro toxicity in balaena mysticetus renal tissue

This study established two- and three-dimensional renal proximal tubular cell cultures of the endangered species bowhead whale (Balaena mysticetus), developed SV40-transfected cultures, and cloned the 61-amino acid open reading frame for the metallothionein protein, the primary binding site for heavy metal contamination in mammals. Microgravity research, modulations in mechanical culture conditions (modeled microgravity), and shear stress have spawned innovative approaches to understanding the dynamics of cellular interactions, gene expression, and differentiation in several cellular systems. These investigations have led to the creation of ex vivo tissue models capable of serving as physiological research analogs for three-dimensional cellular interactions. These models are enabling studies in immune function, tissue modeling for basic research, and neoplasia. Three-dimensional cellular models emulate aspects of in vivo cellular architecture and physiology and may facilitate environmental toxicological studies aimed at elucidating biological functions and responses at the cellular level. Marine mammals occupy a significant ecological niche (72% of the Earth's surface is water) in terms of the potential for information on bioaccumulation and transport of terrestrial and marine environmental toxins in high-order vertebrates. Few ex vivo models of marine mammal physiology exist in vitro to accomplish the aforementioned studies. Techniques developed in this investigation, based on previous tissue modeling successes, may serve to facilitate similar research in other marine mammals.

Whales/genetics/physiology↗

NASA NTRS1994

Paleolimnology of the McMurdo Dry Valleys, Antarctica

The McMurdo Dry Valleys presently contain more than 20 permanent lakes and ponds, which vary markedly in character. All, with the exception of a hypersaline pond, have a perennial ice-cover. The dry valley lakes, and lakes in other ice-free regions of continental Antarctica, are unique on this planet in that they consistently maintain a thick year-round ice cover (2.8-6.0 m) over liquid water. The persistent ice covers minimize wind-generated currents and reduce light penetration, as well as restricting sediment deposition into a lake and the exchange of atmospheric gases between the water column and the atmosphere. From a paleolimnological perspective, the dry valley lakes offer an important record of catchment and environmental changes. These lakes are also modern-day equivalents of periglacial lakes that were common during glacial periods at temperate latitudes. The present lakes are mostly remnants of larger glacial lakes that occupied the valleys in the past, perhaps up to 4.6 Ma ago. Two of the valleys contain evidence of being filled with large glacial lakes within the last 10000 years. Repeated drying and filling events since then have left a characteristic impression on the salt profiles of some lakes creating a unique paleo-indicator within the water column. These events are also marked in the sediments by the concentration and dilution of certain chemical constituents, particularly salts, and are also corroborated by carbonate speciation and oxygen isotope analysis. Stratigraphic analysis of dry valley lake sediments is made difficult by the occurrence of an 'old carbon' reservoir creating spurious radiocarbon dates, and by the high degree of spatial variability in lake sedimentation. From a biological perspective, the lakes are relatively simple, containing various taxa of planktonic and benthic microorganisms, but no higher forms of life, which is an advantage to paleolimnologists because there is no bioturbation in the sediments. Useful biological paleo-indicators found in the sediments include cyanobacterial filament sheaths, diatom frustules and other eukaryotic algal cells, protozoan cysts, photosynthetic pigments, and minerals (e.g. carbonates) associated with microbial activity. Future work will benefit from fully characterizing the connection between the ice covers, environmental conditions, and paleo-indicators, thereby allowing refinement of inferences made concerning the paleoenvironment. New dating techniques need to be tested in this environment to overcome the problems associated with radiocarbon dating. The establishment of a detailed and focused paleolimnological campaign is proposed.

Non-NASA Center↗

NASA NTRS2006

Multisensor Platform Deployment Proposal for International Polar Year (IPY)

High resolution thermal surveys will be acquired to quantify the thermal balance of several active, changing volcanoes which are difficult to monitor for change. Maps of the heat flow on these volcanoes will help in understanding the internal processes changing their surfaces. Correlation of heat flow with volcano deformation and seismicity will help in the understanding of this active region. Remote sensing of composition and biology of warm, bare areas will allow a better understanding of these extreme ecosystems. The thermal survey s and ancillary compositional and biological mapping will be used to focus searches on Mars for similar environments.Mars is a polar desert, much like Antarctica, and one of the few places on Mars where life could exist is in a thermal anomaly like the bare, warm ground areas on the Antarctic volcanoes. We will study the factors making these areas habitable and hone the remote sensing techniques that may be used to detect these features on Mars. Warm areas on Mars (as on the Antarctic volcanoes) are especially hospitable to humans as well, making their discovery on Mars of primary importance for human occupation.

Antarctica↗

NASA NTRS2000

Cellular Responses to Mechanical Stress Selected Contribution: A Three-Dimensional Model for Assessment of in Vitro Toxicity in Balaena Mysticetus Renal Tissue

Goodwin, T. J.↗

NASA NTRS2004

Microbial monitoring of spacecraft and associated environments

Rapid microbial monitoring technologies are invaluable in assessing contamination of spacecraft and associated environments. Universal and widespread elements of microbial structure and chemistry are logical targets for assessing microbial burden. Several biomarkers such as ATP, LPS, and DNA (ribosomal or spore-specific), were targeted to quantify either total bioburden or specific types of microbial contamination. The findings of these assays were compared with conventional, culture-dependent methods. This review evaluates the applicability and efficacy of some of these methods in monitoring the microbial burden of spacecraft and associated environments. Samples were collected from the surfaces of spacecraft, from surfaces of assembly facilities, and from drinking water reservoirs aboard the International Space Station (ISS). Culture-dependent techniques found species of Bacillus to be dominant on these surfaces. In contrast, rapid, culture-independent techniques revealed the presence of many Gram-positive and Gram-negative microorganisms, as well as actinomycetes and fungi. These included both cultivable and noncultivable microbes, findings further confirmed by DNA-based microbial detection techniques. Although the ISS drinking water was devoid of cultivable microbes, molecular-based techniques retrieved DNA sequences of numerous opportunistic pathogens. Each of the methods tested in this study has its advantages, and by coupling two or more of these techniques even more reliable information as to microbial burden is rapidly obtained. Copyright 2004 Springer-Verlag.

Environmental Monitoring/methods↗

NASA NTRS1993

Neural network for processing both spatial and temporal data with time based back-propagation

Neural networks are computing systems modeled after the paradigm of the biological brain. For years, researchers using various forms of neural networks have attempted to model the brain's information processing and decision-making capabilities. Neural network algorithms have impressively demonstrated the capability of modeling spatial information. On the other hand, the application of parallel distributed models to the processing of temporal data has been severely restricted. The invention introduces a novel technique which adds the dimension of time to the well known back-propagation neural network algorithm. In the space-time neural network disclosed herein, the synaptic weights between two artificial neurons (processing elements) are replaced with an adaptable-adjustable filter. Instead of a single synaptic weight, the invention provides a plurality of weights representing not only association, but also temporal dependencies. In this case, the synaptic weights are the coefficients to the adaptable digital filters. Novelty is believed to lie in the disclosure of a processing element and a network of the processing elements which are capable of processing temporal as well as spacial data.

Villarreal, James A.↗

NASA NTRS2000

Ceratopteris richardii: a productive model for revealing secrets of signaling and development

Ceratopteris richardii is an aquatic fern grown in tropical and subtropical regions of the world. It is proven to be a productive model system for studies in the genetics, biochemistry, and cell biology of basic biologic processes that occur in early gametophytic development. It provides several advantages to biologists, especially those interested in gravitational biology, polarity development, and in the genetics of sexual development. It is easy to culture, has a relatively short life cycle, and offers an array of attractive features that facilitate genetic studies. The germination and early development of large populations of genetically identical spores are easy to synchronize, and both the direction of polarity development and cell-level gravity responses can be measured and readily manipulated within the first 24 h of spore development. Although there is no reliable transformation system available yet in Ceratopteris, recent studies suggest that the technique of RNA interference can be used to block translation of specific genes in a related fern, Marsilea, and current studies will soon reveal the applicability of this approach, as well as of other transformation approaches, in Ceratopteris. A recently completed expressed sequence tag (EST) sequencing project makes available the partial sequence of more than 2000 cDNAs, representing a significant percentage of the genes being expressed during the first 24 h of spore germination, when many developmentally interesting processes are occurring. A microarray of these ESTs is being constructed, so especially for those scientists interested in basic cellular phenomena that occur early in spore germination, the availability of the ESTs and of the microarray will make Ceratopteris an even more attractive model system.

NASA Discipline Plant Biology↗

NASA NTRS1977

SPAR project: Counter current distribution of biologicals

Preliminary ground based experiments strongly support the validity of a proposed space experiment in the separation of biological cells by counter current distribution (CCD). It was shown that a low level applied selective field can accelerate the separation of the phases by a very large factor. An electric field therefore should be very effective in space in lieu of gravity for the phase separation step. This appears to solve a central problem in implementing CCD in space and opens the technique to many types of large cell mixtures for which it has not been applicable before due to rapid settling effects. It was determined that electrophoretic migration of the cells, as a possibly disturbing factor, will be of negligible effect. Attenuation of a light beam by scattering generated a signal indicating the course of the phase separation.

Brooks, D. E.↗

NASA NTRS2004

Macromolecular Crystallization in Microgravity

The key concepts that attracted crystal growers, macromolecular or solid state, to microgravity research is that density difference fluid flows and sedimentation of the growing crystals are greatly reduced. Thus, defects and flaws in the crystals can be reduced, even eliminated, and crystal volume can be increased. Macromolecular crystallography differs from the field of crystalline semiconductors. For the latter, crystals are harnessed for their electrical behaviors. A crystal of a biological macromolecule is used instead for diffraction experiments (X-ray or neutron) to determine the three-dimensional structure of the macromolecule. The better the internal order of the crystal of a biological macromolecule then the more molecular structure detail that can be extracted. This structural information that enables an understanding of how the molecule functions. This knowledge is changing the biological and chemical sciences with major potential in understanding disease pathologies. Macromolecular structural crystallography in general is a remarkable field where physics, biology, chemistry, and mathematics meet to enable insight to the basic fundamentals of life. In this review, we examine the use of microgravity as an environment to grow macromolecular crystals. We describe the crystallization procedures used on the ground, how the resulting crystals are studied and the knowledge obtained from those crystals. We address the features desired in an ordered crystal and the techniques used to evaluate those features in detail. We then introduce the microgravity environment, the techniques to access that environment, and the theory and evidence behind the use of microgravity for crystallization experiments. We describe how ground-based laboratory techniques have been adapted to microgravity flights and look at some of the methods used to analyze the resulting data. Several case studies illustrate the physical crystal quality improvements and the macromolecular structural advances. Finally, limitations and alternatives to microgravity and future directions for this research are covered.

Snell, Edward H.↗

NASA NTRSDate not supplied

KSC to EDEN ISS: Outredgeous Lettuce Growth on the Passive Porous Tube Nutrient Delivery System (PPTNDS)

EDEN ISS is a controlled environment greenhouse established near the Neumayer Station III in Antarcticawith the mission to enable fresh crop production for the crew who are working in this remote location. EDEN provides a space to conduct plant biology and related research in a unique and isolated controlled environment (CE), while providing a platform to test developing methods relevant to long duration manned space missions. In 2021, Kennedy Space Center (KSC) scientist Jess Bunchek fulfilled a one-year mission at EDEN. During that time a variety of fresh crops were grown to record horticultural and microbiological data as well as provide the crew with fresh food to eat. One method for crop growth included an experimental irrigation system called the Passive Porous Tube Nutrient Delivery System (PPTNDS) which was developed at KSC as a microgravity-capable plant irrigation system designed to support Space Plant Biology investigations and/or crop growth during space missions. The PPTNDS was employed to grow Outredgeous Lettuce (OL) during two growouts at EDEN, (harvesting at Day After Planting (DAP) 61 and 63) and two growouts at KSC where plant growth and micro sample data were captured. This paper presents the data collected from the PPTNDS and compares it to growth on a Nutrient Film Technique (NFT) hydroponic system operated in the same environment. The result shows a system that grows OL at a reduced rate compared to the NFT system but uses less water and crew interaction to operate.

Jacob Torres↗