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At least 505 records · Page 28

SARS-CoV-2 raw wastewater surveillance from student residences on an urban university campus

The COVID-19 pandemic brought about an urgent need to monitor the community prevalence of infection and detect the presence of SARS-CoV-2. Testing individual people is the most reliable method to measure the spread of the virus in any given community, but it is also the most expensive and time-consuming. Wastewater-based epidemiology (WBE) has been used since the 1960s when scientists implemented monitoring to measure the effectiveness of the Polio vaccine. Since then, WBE has been used to monitor populations for various pathogens, drugs, and pollutants. In August 2020, the University of Tennessee-Knoxville implemented a SARS-CoV-2 surveillance program that began with raw wastewater surveillance of the student residence buildings on campus, the results of which were shared with another lab group on campus that oversaw the pooled saliva testing of students. Sample collection began at 8 am, and the final RT-qPCR results were obtained by midnight. The previous day’s results were presented to the campus administrators and the Student Health Center at 8 am the following morning. The buildings surveyed included all campus dormitories, fraternities, and sororities, 46 buildings in all representing an on-campus community of over 8,000 students. The WBE surveillance relied upon early morning “grab” samples and 24-h composite sampling. Because we only had three Hach AS950 Portable Peristaltic Sampler units, we reserved 24-h composite sampling for the dormitories with the highest population of students. Samples were pasteurized, and heavy sediment was centrifuged and filtered out, followed by a virus concentration step before RNA extraction. Each sample was tested by RT-qPCR for the presence of SARS-CoV-2, using the CDC primers for N Capsid targets N1 and N3. The subsequent pooled saliva tests from sections of each building allowed lower costs and minimized the total number of individual verification tests that needed to be analyzed by the Student Health Center. Our WBE results matched the trend of the on-campus cases reported by the student health center. The highest concentration of genomic copies detected in one sample was 5.06 × 10 7 copies/L. Raw wastewater-based epidemiology is an efficient, economical, fast, and non-invasive method to monitor a large community for a single pathogen or multiple pathogen targets.

60 APPLIED LIFE SCIENCES↗

Air, surface, and wastewater surveillance of SARS-CoV-2; a multimodal evaluation of COVID-19 detection in a built environment

Environmental surveillance of infectious organisms holds tremendous promise to reduce human-to-human transmission in indoor spaces through early detection. In this study we determined the applicability and limitations of wastewater, indoor high-touch surfaces, in-room air, and rooftop exhaust air sampling methods for detecting SARS-CoV-2 in a real world building occupied by residents recently diagnosed with COVID-19. We concurrently examined the results of three 24-hour environmental surveillance techniques, indoor surface sampling, exhaust air sampling and wastewater surveillance, to the known daily census fluctuations in a COVID-19 isolation dormitory. Additionally, we assessed the ability of aerosol samplers placed in the large volume lobby to detect SARS-CoV-2 multiple times per day. Our research reveals an increase in the number of individuals confirmed positive with COVID-19 as well as their estimated human viral load to be associated with statistically significant increases in viral loads detected in rooftop exhaust aerosol samples (p = 0.0413), wastewater samples (p = 0.0323,), and indoor high-touch surfaces (p < 0.001)). We also report that the viral load detected in lobby aerosol samples was statistically higher in samples collected during presence of occupants whose COVID-19 diagnostic tests were confirmed positive via qPCR compared to periods when the lobby was occupied by either contact-traced (suspected positive) individuals or during unoccupied periods (p = 0.0314 and <2e–16). We conclude that each daily (24h) surveillance method, rooftop exhaust air, indoor high-touch surfaces, and wastewater, provide useful detection signals for building owner/operator(s). Furthermore, we demonstrate that exhaust air sampling can provide spatially resolved signals based upon ventilation exhaust zones. Additionally, we find that indoor lobby air sampling can provide temporally resolved signals useful during short duration sampling periods (e.g., 2-4 hours) even with intermittent occupancy by occupants diagnosed with COVID-19.

60 APPLIED LIFE SCIENCES↗

Summary Report for the Analysis of the Sludge Batch 7b (Macrobatch 9) DWPF Pour Stream Glass Sample for Canister S04023

In order to comply with the Defense Waste Processing Facility (DWPF) Waste Form Compliance Plan for Sludge Batch 7b, Savannah River National Laboratory (SRNL) personnel characterized the Defense Waste Processing Facility (DWPF) pour stream (PS) glass sample collected while filling canister S04023. This report summarizes the results of the compositional analysis for reportable oxides and radionuclides and the normalized Product Consistency Test (PCT) results. The PCT responses indicate that the DWPF produced glass that is significantly more durable than the Environmental Assessment glass. Results and further details are documented in SRNL-STI-2013-00462, Revision 1 “Analysis of the DWPF Sludge Batch 7b (Macrobatch 9) Pour Stream Glass Sample.”

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Robot for Investigations and Assessments of Nuclear Areas

RIANA is a remote controlled Robot dedicated for Investigations and Assessments of Nuclear Areas. The development of RIANA is motivated by the need to have at disposal a proven robot, tested in hot cells; a robot capable of remotely investigate and characterise the inside of nuclear facilities in order to collect efficiently all the required data in the shortest possible time. It is based on a wireless medium sized remote carrier that may carry a wide variety of interchangeable modules, sensors and tools. It is easily customised to match specific requirements and quickly configured depending on the mission and the operator's preferences. RIANA integrates localisation and navigation systems. The robot will be able to generate / update a 2D map of its surrounding and exploring areas. The position of the robot is given accurately on the map. Furthermore, the robot will be able to autonomously calculate, define and follow a trajectory between 2 points taking into account its environment and obstacles. The robot is configurable to manage obstacles and restrict access to forbidden areas. RIANA allows an advanced control of modules, sensors and tools; all collected data (radiological and measured data) are displayed in real time in different format (chart, on the generated map...) and stored in a single place so that may be exported in a convenient format for data processing. This modular design gives RIANA the flexibility to perform multiple investigation missions where humans cannot work such as: visual inspections, dynamic localization and 2D mapping, characterizations and nuclear measurements of floor and walls, non destructive testing, samples collection: solid and liquid. The benefits of using RIANA are: - reducing the personnel exposures by limiting the manual intervention time, - minimizing the time and reducing the cost of investigation operations, - providing critical inputs to set up and optimize cleanup and dismantling operations. (authors)

42 ENGINEERING↗

Iodine Air Sampling: Installation and Preliminary Results

The F-Area wetlands are a known sink for radioiodine generated at the Savannah River Site (SRS). Wastewater containing iodine-129 ( 129 I) was discharged into the F-Area Seepage Basins for disposal, but the acidity of the wastewater mobilized some of the contaminants from the basin soil through the vadose zone and into the groundwater; ultimately creating a groundwater plume that extends towards Fourmile Branch and its associated wetland areas. Dissolved radioiodine specifically has migrated to the nearby F-Area wetlands, where it persists as a contaminant of concern. Accumulation and resurfacing of 129 I in the wetlands and nearby areas creates a potential secondary source zone where changes in biogeochemical conditions could result in the release of this sequestered 129 I to the air. Monitoring data has indicated that 129 I has a seasonal behavior at several surface water stations, with high concentrations during summertime and low concentrations during wintertime. Still, it is not known which factors control the seasonal behavior of 129 I. Further, it has been reported that 129 I contributed approximately 23% to the 2019 SRS offsite air pathway dose to the representative person for that year (SRNS, 2020), but air measurements of 129 I have never been taken in the F-Area wetlands. Because air emissions of 129 I from the wetlands have never been investigated, it is not known if this could be a potential release pathway that needs to be included in the site conceptual model. To address these unknowns, the Savannah River National Laboratory (SRNL) has an applied research task to investigate whether 129 I in the wetlands is emitted to the air and, if so, whether air concentrations exhibit seasonal variation. This report presents information about the system recently installed in the F-Area wetlands for measuring 129 I, as well as analysis results for samples collected June through August 2023. Quantification of this constituent will provide a better understanding of the contribution of airborne 129 I from the F-Area wetlands to the offsite dose and better inform ongoing and future remedial actions.

54 ENVIRONMENTAL SCIENCES↗

Sun Grant/DOE Regional Feedstock Partnership: Final Technical Report

The U.S. Department of Energy (DOE) and the Sun Grant Initiative initiated the Regional Feedstock Partnership (referred to as the Partnership) in 2007 primarily to address information gaps associated with the likelihood for realization of the sustainable and reliable production of a billion-tons of biomass annually to support the U.S. bioenergy industry by the year 2030. Publication of The Technical Feasibility of a Billion-Ton Annual Supply (Perlack et al., 2005) in 2005, with its associated yield assumptions, led to the realization that the yield goals and assumptions utilized in that analysis required careful validation. To achieve the overall goal of validating key yield assumptions, the following objectives were developed: 1. Establishment of replicated field trials across regions to determine the impact of crop residue removal (primarily corn and small grains) on future grain yields and soil health. 2. Establishment of replicated field trials of some of the most promising dedicated energy crops (herbaceous annuals and perennials, as well as woody perennials) to demonstrate the potential performance of these feedstocks across the U.S. 3. Assessment of feedstock resources to be used to estimate a sustainable national supply potential. Teams composed of representatives from Land Grant Universities, USDA ARS, DOE National Laboratories, industry, and other federal agencies were identified and assembled to address each of these objectives. For Objective 1, members of the USDA ARS’ Resilient Economic Agricultural Practices (REAP) team combined with university personnel to leverage on-going work and established new trials to determine sustainable corn residue removal rates. In addition, universities across the continental U.S. conducted surveys of published past research to define parameters for the use of cereal grain residue (primarily wheat) as a biomass feedstock. For Objective 2, and based on input from DOE, USDA, Land Grant university scientists, and others, several herbaceous and woody energy crop species were selected for new or further evaluation across wide geographic areas as cellulosic feedstock sources. Species teams were organized to assess the yield potential of candidate herbaceous feedstocks including sorghum, energycane, Miscanthus x giganteus (hereafter referred to as miscanthus), switchgrass, and mixed perennial species (primarily grasses and some legumes) on Conservation Reserve Program (CRP) land. Woody species will be critical components of the bioenergy sector; therefore, species teams were also organized for shrub willow and hybrid poplar (Populus spp.). Field trials of each species were utilized to determine yield potential across the U.S. For Objective 3, a GIS resource team was organized with representatives from each of the five Sun Grant regions. While some regional assessments were made, the multi-institutional team decided that the group from Oregon State University would utilize their PRISM ELM model to estimate national yield potential of the various species evaluated. The following are key outcomes and impacts • The Partnership demonstrated the production potential of diverse herbaceous and woody feedstocks across much of the U.S. for 5-7 consecutive growing seasons. Long-term studies such as this are not commonly possible due to limitations in funding cycles. • Long-term field trials were valuable in demonstrating the importance of agronomic management practices and genetic resources to yield of sorghum, switchgrass, miscanthus, energycane, CRP mixed perennial grasses, poplar, and willow. • National yield potential maps were developed for all species evaluated in the Partnership. • Sustainable corn stover removal rates were quantified for use by producers, harvesters, and commercial lignocellulosic biorefineries. • Data from the Partnership field trials not only validated initial Billion Ton estimates, but were instrumental in developing both the Billion Ton Update in 2011, and especially the 2016 Billion Ton Report. • The Partnership provided the opportunity to assemble and conserve a poplar germplasm collection that contains more than 20,000 clones for use in breeding programs. New clones resulting from crosses made using this germplasm collection have resulted in significantly improved cultivars that could be scaled up and deployed. • Further validation of improved yields of new varieties/cultivars of biomass sorghum, energycane, hybrid poplars, and shrub willows was produced by the Partnership. • The Partnership demonstrated the potential of energycane in northern parts of the southeastern U.S., particularly in relation to sugarcane varieties. • For certain feedstocks (e.g., CRP mixed grasses, switchgrass, willow, and poplar), production economics were ascertained through Partnership field trials. • Biomass samples collected as part of the Partnership are also being used to populate Idaho National Laboratory’s Bioenergy Feedstock Library. • Because of the size and scope of the Partnership, workforce development was a key component and outcome. Numerous undergraduate students (25), graduate students (15 MS and 8 PhD), and postdoctoral researchers (7) were trained by Partnership participants. This will help to prepare individuals for careers in the emerging fields of plant breeding, biomass production, feedstock supply logistics, and biomass conversion processes. • More than 130 scientific publications have been authored by Partnership team members. Numerous other presentations, outreach publications, book chapters, and websites have also been developed to further the continued development of the bioeconomy.

09 BIOMASS FUELS↗

Toward using collective x-ray Thomson scattering to study C–H demixing and hydrogen metallization in warm dense matter conditions

The insulator–metal transition in liquid hydrogen is an important phenomenon to understand the interiors of gas giants, such as Jupiter and Saturn, as well as the physical and chemical behavior of materials at high pressures and temperatures. Here, the path toward an experimental approach is detailed based on spectrally resolved x-ray scattering, tailored to observe and characterize hydrogen metallization in dynamically compressed hydrocarbons in the regime of carbon–hydrogen phase separation. With the help of time-dependent density functional theory calculations and scattering spectra from undriven carbon samples collected at the European x-ray Free-Electron Laser Facility (EuXFEL), we demonstrate sufficient data quality for observing C–H demixing and investigating the presence of liquid metallic hydrogen in future experiments using the reprated drive laser systems at EuXFEL.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Inclusive and differential cross-sections for dilepton $t\overline{t}$ production measured in $\sqrt{s}$ = 13 TeV pp collisions with the ATLAS detector

Differential and double-differential distributions of kinematic variables of leptons from decays of top-quark pairs ($t\bar{t}$) are measured using the full LHC Run 2 data sample collected with the ATLAS detector. The data were collected at a pp collision energy of $\sqrt{s}$ = 13 TeV and correspond to an integrated luminosity of 140 fb –1 . The measurements use events containing an oppositely charged e μ pair and b-tagged jets. The results are compared with predictions from several Monte Carlo generators. While no prediction is found to be consistent with all distributions, a better agreement with measurements of the lepton p T distributions is obtained by reweighting the $t\bar{t}$ sample so as to reproduce the top-quark p T distribution from an NNLO calculation. The inclusive top-quark pair production cross-section is measured as well, both in a fiducial region and in the full phase-space. The total inclusive cross-section is found to be $σ_{t\bar{t}}=$ 829 ± 1 (stat) ± 13 (syst) ± 8 (lumi) ± 2 (beam) pb, where the uncertainties are due to statistics, systematic effects, the integrated luminosity and the beam energy. This is in excellent agreement with the theoretical expectation.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Distribution of natural radionuclides in soils and beach sands of Abana-Çatalzeytin (Kastamonu)

A gamma spectrometric study of distribution of natural radionuclides in soil and beach sand samples collected from the terrestrial and coastal environment of Abana and Çatalzeytin counties of Kastamonu Province in Turkey was performed with the aim of estimating the radiation hazard of the tourist area and the concentrations of {sup 238}U, {sup 232}Th and {sup 40}K were determined. The activity concentrations of {sup 238}U, {sup 232}Th and {sup 40}K were determined in the ranges 14.95–56.0, 46.5–99.4 and 357.5–871.3 Bqkg{sup −1} for soil samples and the mean concentrations were ascertained as 42.34, 71.24 and 624.18 Bqkg{sup −1}, respectively. In sand samples, {sup 238}U, {sup 232}Th and {sup 40}K contents were varied in the ranges of 13.35-41.6, 30.9-53.4 and 275.5-601.3 Bqkg{sup −1} and the mean concentrations were ascertained as 20.57, 45.05 and 411.71 Bqkg{sup −1}, respectively. The mean annual effective doses were calculated as 113.08 and 69.16 µSvy{sup −1} for the soil and sand samples, respectively.

07 ISOTOPE AND RADIATION SOURCES↗

Nuclear thermal source transfer unit, post-blast soil sample drying system

Los Alamos National Laboratory states that its mission is “To solve national security challenges through scientific excellence.” The Science Undergraduate Laboratory Internship (SULI) programs exists to engage undergraduate students in STEM work by providing opportunity to work at DOE facilities. As an undergraduate mechanical engineering intern under the SULI program at Los Alamos during the fall semester of 2016, I had the opportunity to contribute to the mission of the Laboratory while developing skills in a STEM discipline. I worked with Technology Applications, an engineering group that supports non-proliferation, counter terrorism, and emergency response missions. This group specializes in tool design, weapons engineering, rapid prototyping, and mission training. I assisted with two major projects during my appointment Los Alamos. The first was a thermal source transportation unit, intended to safely contain a nuclear thermal source during transit. The second was a soil drying unit for use in nuclear postblast field sample collection. These projects have given me invaluable experience working alongside a team of professional engineers. Skills developed include modeling, simulation, group design, product and system design, and product testing.

42 ENGINEERING↗

Practical Considerations of Moisture in Baled Biomass Feedstocks

Agricultural residues make up a large portion of the immediately available biomass feedstock for renewable energy markets. Current collection and storage methods rely on existing feed and forage practices designed to preserve nutrients and properties of digestibility. Low-cost collection and storage practices that preserve carbohydrates across a range of inbound moisture contents are needed to assure the economic and technical success of the emerging biomass industry. This study examines the movement of moisture in storage and identifies patterns of migration resulting from several on-farm storage systems and their impacts on moisture measurement and dry matter recovery. Baled corn stover and energy sorghum were stored outdoors in uncovered, tarp-covered, or wrapped stacks and sampled periodically to measure moisture and dry matter losses. Interpolation between discrete sampling locations in the stack improved bulk moisture content estimates and showed clear patterns of accumulation and re-deposition. Atmospheric exposure, orientation, and contact with barriers (i.e., soil, tarp, and wrap surfaces) were found to cause the greatest amount of moisture heterogeneity within stacks. Although the bulk moisture content of many stacks remained in the range suitable for aerobic stability, regions of high moisture were sufficient to support microbial activity, thus support dry matter loss. Stack configuration, orientation, and coverage methods are discussed relative to impact on moisture management and dry matter preservation. Additionally, sample collection and data analysis are discussed relative to assessment at the biorefinery as it pertains to stability in storage, queuing, and moisture carried into processing.

09 BIOMASS FUELS↗

Imprint of Anthropogenic Sources and Soil Removal on the Surface Concentration of H2 in the Contiguous US

Hydrogen (H2) is experiencing renewed interest throughout the world as a low carbon fuel alternative or complement to fossil fuels. Significant uncertainties remain regarding the environmental impact of increasing H2 usage, in part due to gaps in our understanding of the H2 atmospheric budget, including the H2 release from industrial activities and the H2 soil removal, the most important sink of H2. This study focuses on H2 dry air mole fractions measured by the NOAA Global Monitoring Laboratory in discrete ambient air samples collected every few days at sites located in the contiguous United States between 2010 and 2022. We take advantage of the long-term observations from this network to study the regional distribution of H2 sources using the potential source contribution function (PSCF). We find that H2 PSCF is consistent with a large anthropogenic source of atmospheric H2 from the incomplete combustion of fossil fuel. However, episodic air masses with high H2 dry air mole fractions (>700 ppb) recorded at some sampling locations in the Western and Southeastern US are not associated with elevated CO, suggesting significant noncombustion anthropogenic or geological sources of H2. Air masses depleted in H2 are recorded in rural or remote continental sites. Our analysis of observations from a tall tower in NE Colorado suggests that the degree of H2 depletion not only reflects differences in soil exposure but also the regional inhibition of the soil sink under arid conditions.

Paulot, Fabien↗

Utah FORGE: Well 16B(78)-32 Core Photographs

This dataset includes images of core samples collected from Utah FORGE well 16B(78)-32. The images are stitched photographs showing the circumferential view of the cores exterior in two dimensions and the individual photos of the core taken at multiple angles used to construct the stitched photos.

15 GEOTHERMAL ENERGY↗

Chelation Modeling of a Plutonium-238 Inhalation Incident Treated with Delayed DTPA

This work describes an analysis, using a previously established chelation model, of the bioassay data collected from a worker who received delayed chelation therapy following a plutonium-238 inhalation. The details of the case have already been described in two publications. The individual was treated with Ca-DTPA via multiple intravenous injections and then nebulizations beginning several months after the intake and continuing for four years. The exact date and circumstances of the intake are unknown. However, interviews with the worker suggested that the intake occurred via inhalation of a soluble plutonium compound. The worker provided daily urine and fecal bioassay samples throughout the chelation treatment protocol, including samples collected before, during, and after the administration of Ca-DTPA. Unlike the previous two publications presenting this case, the current analysis explicitly models the combined biokinetics of the plutonium-DTPA chelate. Further, using the previously established chelation model, it was possible to fit the data through optimizing only the intake (day and magnitude), solubility, and absorbed fraction of nebulized Ca-DTPA. This work supports the hypothesis that the efficacy of the delayed chelation treatment observed in this case results mainly from chelation of cell-internalized plutonium by Ca-DTPA (intracellular chelation). It also demonstrates the validity of the previously established chelation model. As the bioassay data were modified to ensure data anonymization, the calculation of the “true” committed effective dose was not possible. However, the treatment-induced dose inhibition (in percentage) was calculated.

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTAN↗

Review of Time-Series Data for the Agra Interim Measure, Fall 2013

The Commodity Credit Corporation of the U.S. Department of Agriculture (CCC/USDA) operated a grain storage facility at Agra, Kansas, from the 1950s to the early 1970s. No structures remain on the property, and the land is used for agricultural purposes, specifically wheat production. The property is currently owned by the Kyle Railroad Co. and is leased to Mr. Herb VanEaton. The Pro-Ag Marketing grain storage facility is directly south of the former CCC/USDA facility. In 1985, carbon tetrachloride was detected in groundwater samples collected from Agra public water supply wells PWS-3 and PWS-4, located approximately 2,000 ft south of the former CCC/USDA facility and approximately 1,800 ft south of the Pro-Ag Marketing Facility, at levels below the maximum contaminant level of 5.0 μg/L. In 1986, in response to an increase in carbon tetrachloride to 12 μg/L in well PWS-3, the city was required to remove both PWS-3 and PWS-4 from service. The Agra public water supply system is served by three other wells (PWS-1, PWS-2, and PWS-5) that are outside the area of contamination. In 2005, the Environmental Science Division of Argonne National Laboratory conducted a comprehensive investigation of potential contaminant source areas at Agra, on behalf of the CCC/USDA. Data collected during the 2005 investigation identified three primary source areas for the carbon tetrachloride contamination (Argonne 2006). One source is located on the former CCC/USDA property, and the other two sources are on property owned by Pro-Ag Marketing. In 2008-2009, to address the carbon tetrachloride contamination detected on its former property, the CCC/USDA initiated source area cleanup as an interim measure (IM) in accord with the document Interim Measure Work Plan/Design for Agra, Kansas (IMWP/D; Argonne 2008). The cleanup employs five large-diameter boreholes (LDBs) that were excavated by auger drilling. The purposes of these borings were to (1) physically remove soils impacted by carbon tetrachloride from the most contaminated area for treatment by onsite land farming and (2) provide locations for the installation of coupled soil vapor extraction (SVE) and air sparging (AS) wells to facilitate in situ treatment of the remaining carbon tetrachloride in the soils and groundwater. Construction data for the SVE wells, the AS wells, and groundwater and soil gas observation points installed to monitor the progress of the cleanup (as reported on the Kansas Review of Time-Series Data for the Agra Interim Measure 1-2 Version 01, 10/24/13 WWC-5 forms for these installations) are summarized in Table 1.1. The locations of the wells are shown in Figure 1.1. The IMWP/D was approved by the Kansas Department of Health and Environment in November 2008 (KDHE 2008), and operation of the SVE/AS systems began in May 2009. With the approval of the KDHE (2009), landfarming operations to treat the contaminated soils excavated from the LDBs were completed on October 12, 2010. Since start-up, the IM activities have been documented periodically (Argonne 2009a,b,c; Argonne 2010a,b,c,d, 2011a,b,c, 2012, 2013a,b), in accord with the requirements of the KDHEapproved IMWP/D (Argonne 2008). Each of the reports primarily summarizes the operations and maintenance of the SVE and AS equipment and the results of sampling of the groundwater, soil gas, and system effluents for analyses for volatile organic compounds (VOCs) over a specific 3- to 6-month period. Hence, the focus is the incremental performance of the IM. To complement these documents, this report provides a broader perspective by examining the collective data acquired since start-up on May 29, 2009, and through June 2013. The objectives of this approach are to evaluate possible longer-term trends in (1) the operational characteristics of the remedial systems and (2) the corresponding responses in the subsurface environment and their potential relationships to the progress of the cleanup. To facilitate this investigation, time-series diagrams have been developed to illustrate key parameter data for the SVE and AS systems, as well as sampling and additional supporting data obtained from the monitoring program. An overview of the information compiled is in Section 2. In Section 3, observations drawn from these data are discussed. The principal findings of the evaluation and recommendations for consideration are summarized in Sections 4 and 5, respectively.

54 ENVIRONMENTAL SCIENCES↗

Variation in microbial community structure correlates with heavy-metal contamination in soils decades after mining ceased

Microorganisms play vital roles in Earth's biogeochemical cycles. Identifying disturbances in microbial communities due to anthropogenic contamination can provide insights into the health of ecosystems. Picher, Oklahoma, was the site of large-scale mining operations for Pb, Zn, and other heavy metals until the mid-1950s, operating within the Tri-State Mining District (TSMD) of Missouri, Kansas and Oklahoma. Although mining ceased decades ago, high concentrations of heavy metals (>1000 ppm) remain in area soil and water systems. Previously, we mapped metal concentrations on samples collected from mine tailings in Picher and along cardinal-direction transects within an 8.05-km radius of the town. To elucidate changes in microbial community structure due to regional metal contamination, 16S rRNA gene sequences and qPCR calculations of total Bacteria and Archaea were analyzed against these metal concentrations. Bacteria were negatively and significantly correlated with Pb, Cd, Zn, and Mg; however, Archaea was only significantly and positively correlated with pH. Illumina sequencing of 16S rRNA genes showed significant differences in microbial communities of chat and west transect samples. Comparison of soil chemistry with community structure indicated that Al, Pb, Cd, and Zn significantly impacted community composition and distribution of individual OTUs. Mapping the distribution of heavy-metal contamination and microbial communities in these soils represents the first step in understanding effects of long-term, heavy-metal contamination at a basic trophic level.

59 BASIC BIOLOGICAL SCIENCES↗

Evaluation of Near Field Atmospheric Dispersion Around Nuclear Facilities Using a Lorentzian Distribution Methodology

Atmospheric dispersion modeling within the near field of a nuclear facility typically applies a building wake correction to the Gaussian plume model, whereby a point source is modeled as a plane source. The plane source results in greater near field dilution and reduces the far field effluent concentration. However, the correction does not account for the concentration profile within the near field. Receptors of interest, such as the maximally exposed individual, may exist within the near field and thus the realm of building wake effects. Furthermore, release parameters and displacement characteristics may be unknown, particularly during upset conditions. Therefore, emphasis is placed upon the need to analyze and estimate an enveloping concentration profile within the near field of a release. This investigation included the analysis of 64 air samples collected over 128 wk. Variables of importance were then derived from the measurement data, and a methodology was developed that allowed for the estimation of Lorentzian-based dispersion coefficients along the lateral axis of the near field recirculation cavity; the development of recirculation cavity boundaries; and conservative evaluation of the associated concentration profile. The results evaluated the effectiveness of the Lorentzian distribution methodology for estimating near field releases and emphasized the need to place air-monitoring stations appropriately for complete concentration characterization. Additionally, the importance of the sampling period and operational conditions were discussed to balance operational feedback and the reporting of public dose.

54 ENVIRONMENTAL SCIENCES↗