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NASA NTRS2007

Mesospheric Dynamical Changes Induced by the Solar Proton Events in October-November 2003

The very large solar storms in October-November 2003 caused solar proton events (SPEs) at the Earth that impacted the upper atmospheric polar cap regions. The Thermosphere Ionosphere Mesosphere Electrodynamic General Circulation Model (TIME-GCM) was used to study the atmospheric dynamical influence of the solar protons that occurred in Oct-Nov 2003, the fourth largest period of SPEs measured in the past 40 years. The highly energetic solar protons caused ionization, as well as dissociation processes, and ultimately produced odd hydrogen (HOx) and odd nitrogen (NOy). Significant short-lived ozone decreases (10-70%) followed these enhancements of HOx and NOy and led to a cooling of most of the lower mesosphere. This cooling caused an atmospheric circulation change that led to adiabatic heating of the upper mesosphere. Temperature changes up to plus or minus 2.6 K were computed as well as wind (zonal, meridional, vertical) perturbations up to 20-25% of the background winds as a result of 22 the solar protons. The solar proton-induced mesospheric temperature and wind perturbations diminished over a period of 4-6 weeks after the SPEs. The Joule heating in the mesosphere, induced by the solar protons, was computed to be relatively insignificant for these solar storms with most of the temperature and circulation perturbations caused by ozone depletion in the sunlit hemisphere.

Jackman, Charles H.↗
NASA NTRS2007

Quantum Effects in Cosmochemistry: Complexation Energy and Van Der Waals Radii

The subject of quantum effects in cosmochemistry was recently addressed with the goal of understanding how they contribute to Q-phase noble gas abundances found in meteorites. It was the pursuit of the Q-phase carrier of noble gases and their anomalous abundances that ultimately led to the identification, isolation, and discovery of presolar grains. In spite of its importance, Q-phase investigations have led a number of authors to reach conclusions that do not seem to be supported by quantum chemistry. In view of the subject's fundamental significance, additional study is called for. Two quantum properties of Q-phase candidates known as endohedral carbon-cage clathrates such as fullerenes will be addressed here. These are complexation energy and instability induced by Pauli blocking (exclusion principle).

Mittlefehldt, D. W.↗
NASA NTRS2006

Implications of RHESSI Observations for Solar Flare Models and Energetics

Observations of solar flares in X-rays and gamma-rays provide the most direct information about the hottest plasma and energetic electrons and ions accelerated in flares. The Ramaty High Energy Solar Spectroscopic Imager (RHESSI) has observed over 18000 solar flares in X-rays and gamma-rays since its launch in February of 2002. RHESSI observes the full Sun at photon energies from as low as 3 keV to as high as 17 MeV with a spectral resolution on the order of 1 keV. It also provides images in arbitrary bands within this energy range with spatial resolution as good as 3 seconds of arc. Full images are typically produced every 4 seconds, although higher time resolution is possible. This unprecedented combination of spatial, spectral, and temporal resolution, spectral range and flexibility has led to fundamental advances in our understanding of flares. I will show RHESSI and coordinated observations that confirm coronal magnetic reconnection models for eruptive flares and coronal mass ejections, but also present new puzzles for these models. I will demonstrate how the analysis of RHESSI spectra has led to a better determination of the energy flux and total energy in accelerated electrons, and of the energy in the hot, thermal flare plasma. I will discuss how these energies compare with each other and with the energy contained in other flare-related phenomena such as interplanetary particles and coronal mass ejections.

Holman, Gordon D.↗
NASA NTRS2006

The Development Of Enabling Technologies For Submillimeter-Wave Remote Sensing of Ice Clouds From Space

This paper presents the chronological development of technologies and techniques that have led to a satellite mission concept aimed at quantifying the temporal and spatial distributions of upper tropospheric ice clouds. The Submillimeter-wave and Infrared Ice Cloud Experiment (SIRICE) is an Earth System Science Pathfinder mission concept designed to improve our understanding of the upper tropospheric water cycle and its coupling to the Earth s radiation budget. Ice outflow from convective storm systems is known to play an important role in regional energy budgets; however, ice generation and subsequent precipitation and sublimation are poorly quantified. SIRICE will provide measurements of ice cloud distributions and microphysical properties which are needed for understanding the crucial link between the hydrologic and energy cycles. The SIRICE measurement platform is comprised of two integrated instruments, the Submillimeter/millimeter-wave radiometer (SM4) and the Infrared Cloud Ice Radiometer (IRCIR). The primary instrument is the SM4, a conical scanner that provides a 1600 km swath of the Earth's surface at 53 degree incidence. The SM4 has 6 linearly polarized receivers measuring 12 spectral bands centered at 183 GHz, 325 GHz, 448 GHz, 643 GHz and 874 GHz; two receivers at 643 GHz measure horizontal and vertical polarizations. Submillimeter-wavelengths are well suited to the remote sensing of ice clouds due to the relative size of the wavelengths to particle sizes. Upwelling emission from lower tropospheric water vapor is scattered by the ice clouds thus causing a brightness temperature depression at submillimeter wavelengths. The IRCIR is a push broom imager with approximately 1500 km swath and spectral channels at 11 and 12 micrometers. This combination of coincident infrared and submillimeter-wavelength measurements were chosen because of its ability to provide retrieval of ice water path and median particle size for a wide range of ice clouds from thin cirrus to thick anvil structures. Over the past decade there has been a parallel development of submillimeter-wave technologies, demonstration instruments, and remote sensing techniques that have led to the present SIRICE mission concept. Mapping of these developmental paths reveals the origins, rational and maturity of features of the SIRICE payload such as its channel selection, compact design, and multipoint calibration. This presentation traces the evolution of the SIRICE mission concept from the early 1990's to its present status.

Racette, Paul↗
NASA NTRS2007

The Columbia Accident Investigation and The NASA Glenn Ballistic Impact Laboratory Contributions Supporting NASA's Return to Flight

On February 1, 2003, the Space Shuttle Columbia broke apart during reentry, resulting in loss of the vehicle and its seven crewmembers. For the next several months, an extensive investigation of the accident ensued involving a nationwide team of experts from NASA, industry, and academia, spanning dozens of technical disciplines. The Columbia Accident Investigation Board (CAIB), a group of experts assembled to conduct an investigation independent of NASA, concluded in August, 2003 that the most likely cause of the loss of Columbia and its crew was a breach in the left wing leading edge Reinforced Carbon-Carbon (RCC) thermal protection system initiated by the impact of thermal insulating foam that had separated from the orbiters external fuel tank 81 seconds into the mission's launch. During reentry, this breach allowed superheated air to penetrate behind the leading edge and erode the aluminum structure of left wing, which ultimately led to the breakup of the orbiter. The findings of the CAIB were supported by ballistic impact tests, which simulated the physics of External Tank Foam impact on the RCC wing leading edge material. These tests ranged from fundamental material characterization tests to full-scale Orbiter Wing Leading Edge tests. Following the accident investigation, NASA spent the next 18 months focused on returning the shuttle safely to flight. In order to fully evaluate all potential impact threats from the many debris sources on the Space Shuttle during ascent, NASA instituted a significant impact testing program. The results from these tests led to the validation of high-fidelity computer models, capable of predicting actual or potential Shuttle impact events, were used in the certification of STS-114, NASA s Return to Flight Mission, as safe to fly. This presentation will provide a look into the inner workings of the Space Shuttle and a behind the scenes perspective on the impact analysis and testing done for the Columbia Accident Investigation and NASA's Return to Flight programs. In addition, highlights from recent Shuttle missions are presented.

Melis, Matthew E.↗
NASA NTRS2006

EOS MLS Science Data Processing System: A Description of Architecture and Capabilities

This paper describes the architecture and capabilities of the Science Data Processing System (SDPS) for the EOS MLS. The SDPS consists of two major components--the Science Computing Facility and the Science Investigator-led Processing System. The Science Computing Facility provides the facilities for the EOS MLS Science Team to perform the functions of scientific algorithm development, processing software development, quality control of data products, and scientific analyses. The Science Investigator-led Processing System processes and reprocesses the science data for the entire mission and delivers the data products to the Science Computing Facility and to the Goddard Space Flight Center Earth Science Distributed Active Archive Center, which archives and distributes the standard science products.

Microwave Limb Sounder (MLS)↗
NASA NTRS2006

A Numerical Study of Hurricane Erin (2001): Shear and the Organization of Eyewall Vertical Motion - Part II

A high-resolution numerical simulation of Hurricane Erin (2001) is used to examine the organization of vertical motion in the eyewall and how that organization responds to a large and rapid increase in the environmental vertical wind shear and subsequent decrease in shear. During the early intensification period, prior to the onset of significant shear, the upward motion in the eyewall was concentrated in small-scale convective updrafts that formed in association with regions of concentrated vorticity (herein termed mesovortices) with no preferred formation region in the eyewall. Asymmetric flow within the eye was weak. As the shear increased, an azimuthal wavenumber 1 asymmetry in storm structure developed with updrafts tending to form on the downshear to downshear-left side of the eyewall. Continued intensification of the shear led to increasing wavenumber 1 asymmetry, large vortex tilt, and a change in eyewall structure and vertical motion organization. During this time, the eyewall structure was dominated by a vortex couplet with a cyclonic (anticyclonic) vortex on the downtilt-left (downtilt-right) side of the eyewall and strong asymmetric flow across the eye that led to strong mixing of eyewall vorticity into the eye. Upward motion was concentrated over an azimuthally broader region on the downtilt side of the eyewall, upstream of the cyclonic vortex, where low-level environmental inflow converged with the asymmetric outflow from the eye. As the shear diminished, the vortex tilt and wavenumber 1 asymmetry decreased, while the organization of updrafts trended back toward that seen during the weak shear period.

Braun, Scott A.↗
NASA NTRS2007

Overview of NASA Langley's Piezoelectric Ceramic Packaging Technology and Applications

Over the past decade, NASA Langley Research Center (LaRC) has developed several actuator packaging concepts designed to enhance the performance of commercial electroactive ceramics. NASA LaRC focused on properly designed actuator and sensor packaging for the following reasons, increased durability, protect the working material from the environment, allow for proper mechanical and electrical contact, afford "ready to use" mechanisms that are scalable, and develop fabrication methodology applicable to any active material of the same physical class. It is more cost effective to enhance or tailor the performance of existing systems, through innovative packaging, than to develop, test and manufacture new materials. This approach led to the development of several solid state actuators that include THUNDER, the Macrofiber Composite or (MFC) and the Radial Field Diaphragm or (RFD). All these actuators are fabricated using standard materials and processes derived from earlier concepts. NASA s fabrication and packaging technology as yielded, piezoelectric actuators and sensors that are easy to implement, reliable, consistent in properties, and of lower cost to manufacture in quantity, than their predecessors (as evidenced by their continued commercial availability.) These piezoelectric actuators have helped foster new research and development in areas involving computational modeling, actuator specific refinements, and engineering system redesign which led to new applications for piezo-based devices that replace traditional systems currently in use.

Bryant, Robert G.↗
NASA NTRS2007

Semianalytical Propagation of Satellite Orbits about an Arbitrary Central Body

Precision mean element (PME) satellite theories play a key role in orbit dynamics analyses. These theories employ: nonsingular orbital elements comprehensive force models Generalized Method of Averaging Numerical interpolation concepts The Draper Semianalytical Satellite Theory (DSST) (Refs. 1 - 6), whose development was led by the author, and the independently-developed Universal Semianalytical Method (USM) (Ref. 7) are examples of such theories. These theories provide the capability to tailor the force modeling to meet the desired computational speed vs. accuracy trade-off. The flexibility of such theories is demonstrated by their ability to include complicated atmosphere density models and spacecraft models in the perturbation theory context. The value of high speed satellite theories, in this era of computational plenty, is that they allow new ways of looking at astrodynamical problems such as orbit design (Refs. 8, 9) and atmosphere density updating (Refs. 10, 11). In the mid to late-1980 s, the geodynamics community led the development of very precise geopotential models such as GEM T2 and GEM T3 (Ref. 12), and with the subsequent analysis of the TOPEX flight data, JGM-2 and JGM-3 (Ref. 13). These were high degree and order geopotentials, at least 50 x 50. In 1993, the DSST implementation in the GTDS program was extended to include the 50 x 50 geopotential models (Ref. 14). The 50 x 50 geopotential, J2000 integration coordinate system, and solid Earth tide capabilities were integrated in GTDS by Scott Carter (Ref. 15). This capability demonstrated 1 m accuracy versus the TOPEX Precise Orbit Ephemerides. Subsequently the DSST Standalone program was also extended to include high degree and order geopotential models (Ref. 5). More recently GTDS has been hosted in the Linux PC environment. However, all of these efforts have been limited to modeling the motion of an artificial Earth satellite. They did not consider the additional complexities associated with lunar, planetary, or other natural satellite orbiters. Such complexities include: additional coordinate systems (associated with the direction of the north pole of rotation and the prime meridian of the new central bodies) (Ref. 16) normalized gravity model coefficients (desirable for high degree and order fields) (Ref. 17) indirect oblateness

Cefola, Paul J.↗
NASA NTRS2006

Multispectral Microimager for Astrobiology

A primary goal of the astrobiology program is the search for fossil records. The astrobiology exploration strategy calls for the location and return of samples indicative of environments conducive to life, and that best capture and preserve biomarkers. Successfully returning samples from environments conducive to life requires two primary capabilities: (1) in situ mapping of the mineralogy in order to determine whether the desired minerals are present; and (2) nondestructive screening of samples for additional in-situ testing and/or selection for return to laboratories for more in-depth examination. Two of the most powerful identification techniques are micro-imaging and visible/infrared spectroscopy. The design and test results are presented from a compact rugged instrument that combines micro-imaging and spectroscopic capability to provide in-situ analysis, mapping, and sample screening capabilities. Accurate reflectance spectra should be a measure of reflectance as a function of wavelength only. Other compact multispectral microimagers use separate LEDs (light-emitting diodes) for each wavelength and therefore vary the angles of illumination when changing wavelengths. When observing a specularly-reflecting sample, this produces grossly inaccurate spectra due to the variation in the angle of illumination. An advanced design and test results are presented for a multispectral microimager which demonstrates two key advances relative to previous LED-based microimagers: (i) acquisition of actual reflectance spectra in which the flux is a function of wavelength only, rather than a function of both wavelength and illumination geometry; and (ii) increase in the number of spectral bands to eight bands covering a spectral range of 468 to 975 nm.

microimager↗
NASA NTRS2008

Material Density Distribution of Small Debris in Earth Orbit

Over 200 spacecraft and rocket body breakups in Earth orbit have populated that regime with debris fragments in the sub-micron through meter size range. Though the largest debris fragments can cause significant collisional damage to active (operational) spacecraft, these are few and trackable by radar. Fragments on the order of a millimeter to a centimeter in size are as yet untrackable. But this smaller debris can result in damage to critical spacecraft systems and, under the worst conditions, fragmenting collision events. Ongoing research at the NASA Orbital Debris Program Office on the sources of these small fragments has focused on the material components of spacecraft and rocket bodies and on breakup event morphology. This has led to fragment material density estimates, and also the beginnings of shape categorizations. To date the NASA Standard Breakup Model has not considered specific material density distinctions of small debris. The basis of small debris in that model is the fourth hypervelocity impact event of the Satellite Orbital Debris Characterization Impact Test (SOCIT) series. This test targeted a flight-ready, U.S. Transit navigation satellite with a solid aluminum sphere impactor. Results in this event yield characteristic length (size) and area-to-mass distributions of fragments smaller than 10 cm in the NASA model. Recent re-analysis of the SOCIT4 small fragment dataset highlighted the material-specific characteristics of metals and non-metals. Concurrent analysis of Space Shuttle in-situ impact data showed a high percentage of aluminum debris in shuttle orbit regions. Both analyses led to the definition of three main on-orbit debris material density categories -low density (< 2 g/cc), medium density (2 to 6 g/cc), and high density (> 6 g/cc). This report considers the above studies in an explicit extension of the NASA Standard Breakup Model where separate material densities for debris are generated and these debris fragments are propagated in Earth orbit. The near Earth environment is thus parameterized by debris density percentages within subsections of that environment. This model version is used in the upgraded NASA Orbital Debris Engineering Model (ORDEM).

Krisko, P. H.↗
NASA NTRS2006

Flutter of High-Speed Civil Transport Flexible Semispan Model: Time-Frequency Analysis

Time/frequency analysis of fluctuations measured by pressure taps and strain gauges in the experimental studies of the flexible semispan model of a high-speed civil transport wing configuration is performed. The interest is in determining the coupling between the aerodynamic loads and structural motions that led to the hard flutter conditions and loss of the model. The results show that, away from the hard flutter point, the aerodynamic loads at all pressure taps near the wing tip and the structural motions contained the same frequency components. On the other hand, in the flow conditions leading to the hard flutter, the frequency content of the pressure fluctuations near the leading and trailing edges varied significantly. This led to contribution to the structural motions over two frequency ranges. The ratio of these ranges was near 2:1, which suggests the possibility of nonlinear structural coupling.

Chabalko, Christopher C.↗
NASA NTRS2008

Neutron Stars and Black Holes Seen with the Rossi X-Ray Timing Explorer (RXTE)

Astrophysical X-rays bring information about location, energy, time, and polarization. X-rays from compact objects were seen in the first explorations to vary in time. Eclipses and pulsations have simple explanations that identified the importance of X-ray binaries and magnetic neutron stars in the first decade of X-ray astronomy. The dynamics of accretion onto stellar and supermassive black holes and onto neutron stars with relatively low magnetic fields shows up as more complex variations, quasi-periodic oscillations, noise with characteristic frequency spectra, broad-band changes in the energy spectra. To study these variations, RXTE instruments needed to have large area and operational flexibility to find transient activity and observe when it was present. Proportional counters and Phoswich scintillators provided it in a modest mission that has made textbook level contributions to understanding of compact objects. The first seen, and the brightest known, X-ray binary, Sco X-1 is one of a class of neutron stars with low mass companions. Before RXTE, none of these had been seen to show pulsations, though they were hypothesized to be the precursors of radio pulsars with millisecond periods and low magnetic fields. RXTE's large area led to identifying coherent millisecond pulsars in a subset which are relatively faint transients. It also led to identifying short episodes of pulsation during thermonuclear bursts, in sources where a steady signal is not seen. The X-ray stage verifies the evolution that produces millisecond radio pulsars.Masses and radii of neutron stars are being determined by various techniques, constraining the equation of state of matter at nuclear densities. Accretion should lead to a range of neutron star masses. An early stage of superstrong magnetic field neutron stars is now known to produce X-ray and gamma-ray bursts in crust quakes and magnetic field reconnection releases of energy. Soft Gamma Repeaters, Anomolous X-ray Pulsars, and high magnetic field rotation-powered pulsars are all now called magnetars, because they have pulse periods indicating they are slowing down as they would with magnetic dipole radiation for a surface field above 5x1013 gauss. The accretion disk has been connected to the launching of radio jets from black holes, and even from neutron stars. Estimates of the angular momenta of black holes are being made from different approaches, modelling a high frequency oscillation that may be related to how close the inner part of the accretion disk is to the black hole, modelling the continua spectra of the X-ray emission, and modeling the emission of red-shifted iron that may be emitted from the accretion disk. These investigations require early discovery of the black hole transient with the All Sky Monitor on RXTE or other monitoring information, frequent extended observations, and coordinated observations with missions that give higher energy resolution, or radio and infrared information.

Swank, Jean↗
NASA NTRS2006

The Hyper-X Antenna Radiation Pattern Tests and Radio Frequency System Redesign

Testing was performed to determine the antenna radiation pattern measurements of the Hyper-X, X-43 ship 2 aircraft. One test was conducted at the Air Force Flight Test Center (AFFTC) Benefield Anechoic Facility (BAF) on January 24-January 27, 2000. A second test was done at MicroCraft in Tullahoma Tennessee by the Naval Air Weapons Center (NAWC) China Lake, California on September 8, 2001. The overall test objective was to gather qualitative antenna radiation pattern data from installed antennas on the HXRV. This objective was accomplished by collecting antenna radiation patterns at selected elevations of the HXRV. The test data was used to identify areas of RF coverage and assist in positioning the P-3 aircraft for best RF reception. The antenna pattern data presented nulls and possible low RF reception areas around the aircraft. This led to a redesign of the RF system. The redesigned system provided redundancy in case a telemetry transmitter should fail. The redesign provided more complete RF coverage within the antenna's capabilities. A second look at the flight path and necessary data collection led to a change in the beacon transponder system.

Hodge, Mark W.↗
NASA NTRS2009

Megafans as Hydrous Environments

The mesoscale sedimentary environment known as the megafan, is a low-angle, partial cone of fluvial sediment generated where a river enters an unconfined basin where it begins the process of avulsing over wide areas. In shifting to different positions, the river lays down a partial cone of sediment and establishes a characteristic radial pattern of paleo courses. The apparent paucity of sedimentary bodies obviously tied to martian outflow channels may also relate to the difficulty of recognition due to their sheer size and featurelessness. However, the existence of megafans on Mars is being examined now that their ubiquity and characteristics on Earth are better understood. Accordingly we suggest two likely candidates on Mars: Maja Valles fluvial cone and Amazonis Planitia fluvial sedimentary bodies. Two cryptic examples from Amazonis Planitia may be important for understanding subsurface hydrous accumulation. For at least some of its history, discharges from Mangala Valles likely resulted in megafans. Distances from the end of Mangala Valles to the northern (low) margin of the planitia are very large, a fact that has suggested that fluvial emplacement was unlikely. However, the megafan model shows that long megafan radii are indeed feasible. It has been suggested further that discharge from Labou Vallis (8.5S 154.5W) must have led to fluvial sedimentation in the planitia. We suggest that during locally non-lacustrine/ocean phases, this sedimentation would have occurred in the form of megafans. However, the megafan model shows that long megafan radii are indeed feasible. It has been suggested further that discharge from Labou Vallis (8.5S 154.5W) must have led to fluvial sedimentation in the planitia. We suggest that during locally non-lacustrine/ocean phases, this sedimentation would have occurred in the form of megafans. Megafans emanating from Marte, Mangala and Labou valles have probably contributed to hydrous near-subsurface environments--in their distal reaches, i.e. along the northern, eastern and southeastern margins of Amazonis Planitia at various times. Following a new terrestrial analog, we conclude groundwater has at times accumulated preferentially beneath distal slopes of the Maja Valles feature, and along the northern, eastern and southeastern margins of Amazonis Planitia.

Wilkinson, M. Justin↗
NASA NTRS2008

Automated Planning and Scheduling for Orbital Express (151)

The challenging timeline for DARPA's Orbital Express mission demanded a flexible, responsive, and (above all) safe approach to mission planning. Because the mission was a technology demonstration, pertinent planning information was learned during actual mission execution. This information led to amendments to procedures, which led to changes in the mission plan. In general, we used the ASPEN planner scheduler to generate and validate the mission plans. We enhanced ASPEN to enable it to reason about uncertainty. We also developed a model generator that would read the text of a procedure and translate it into an ASPEN model. These technologies had a significant impact on the success of the Orbital Express mission.

autonomy↗
NASA NTRS2009

Psychrotolerant Anaerobes from Lake Podprudnoe, Antarctica and Penguin Spheniscus demersus Colony, South Africa

The study of a sample collected from a wind-made ice sculpture near Lake Podprudnoe, Antarctica led to the isolation of the psychrotolerant strain ISLP-3. Cells of the new isolate are vibrio-shaped that measure 0.5 x 1.0-3.0 micron in size. Growth occurs within the temperature range 5-35 C with the optimum at 22 C. Salinity range for growth is 0-2 % NaCl with the optimum at 0.25 %. The new isolate grows within a pH range from 6.0 to 9.5 with the optimum at 7.5. Strain ISLP-3 is saccharolytic, growing on the following substrates: D-glucose, D-ribose, D-fructose, D-arabinose, maltose, sucrose, D-trehalose, D-mannose, D-cellobiose, lactose, starch, chitin, triethylamine, N-acetylglucosamine, and urea. The best growth occurred on D-cellobiose. An environmental sample of pond water near a colony of the endemic species of African penguins, Spheniscus demersus, was collected in February 2008 and delivered directly to the Astrobiology laboratory at NSSTC. The microbiological study of this sample led to the isolation of two psychrotolerant strains ARHSd-7G and ARHSd-9G. Both strains are strictly anaerobic bacteria and are able to grow at high pH and low temperatures. The cells of strain ARHSd-7G are motile, vibrio-shaped, spore-forming cells. Optimal growth of this strain occurs at 30 C, 3 % NaCl, and pH 8.9. The isolate ARHSd-7G combines sugarlytic and proteolytic metabolisms, growing on some proteolysis products including peptone and yeast extract and a number of sugars. The second isolate, ARHSd-9G, exhibits thin, elongated rods that measure 0.4 x 3-5 micron. The cells are motile and spore-forming. Optimal growth of strain ARHSd-9G occurs at 30 C, 1.75 % NaCl, and pH 8.5. The strain ARHSd-9G is sugarlytic, growing well on substrates such as D-glucose, sucrose, D-cellobiose, maltose, fructose, D-mannose, and trehalose (the only exception is positive growth on yeast extract). In this report, the physiological and morphological characteristics of the novel psychrotolerant, alkaliphilic, and neutrophilic isolates from the Antarctica 2008 expedition will be discussed.

Guisler, Melissa↗
NASA NTRS2009

Optoelectronic pH Meter: Further Details

A collection of documents provides further detailed information about an optoelectronic instrument that measures the pH of an aqueous cell-culture medium to within 0.1 unit in the range from 6.5 to 7.5. The instrument at an earlier stage of development was reported in Optoelectronic Instrument Monitors pH in a Culture Medium (MSC-23107), NASA Tech Briefs, Vol. 28, No. 9 (September 2004), page 4a. To recapitulate: The instrument includes a quartz cuvette through which the medium flows as it is circulated through a bioreactor. The medium contains some phenol red, which is an organic pH-indicator dye. The cuvette sits between a light source and a photodetector. [The light source in the earlier version comprised red (625 nm) and green (558 nm) light-emitting diodes (LEDs); the light source in the present version comprises a single green- (560 nm)-or-red (623 nm) LED.] The red and green are repeatedly flashed in alternation. The responses of the photodiode to the green and red are processed electronically to obtain the ratio between the amounts of green and red light transmitted through the medium. The optical absorbance of the phenol red in the green light varies as a known function of pH. Hence, the pH of the medium can be calculated from the aforesaid ratio.

Jeevarajan, Antony S.↗